The impact of ZnO and TiO2 on the stability of clotrimazole under UVA irradiation: Identification of photocatalytic degradation products and in vitro cytotoxicity assessment

Bioactive compounds from natural sources have been extensively studied as potential therapeutic agents, due to their chemical, structural, and biological properties. Among them, hydroxycinnamic acids emerge with a very interesting pharmacological potential to prevent and treat several diseases, including cancer. However, while some studies support their anticancer activity, other reports indicate otherwise. Since the impact of these compounds on renal cancer is not well established, further studies are needed to better understand their biological effects and their potential therapeutic uses in this cancer. In this work, the cytotoxicity of three hydroxycinnamic acids: ferulic, caffeic and p-coumaric acids, was evaluated on 786-O human renal cancer cells by MTT and CV assays. At concentrations up to 250 µM, none of the studied natural compounds showed cytotoxic effects to 786-O cell line. At higher concentrations (500 and 1000 µM), while ferulic acid also did not significantly influence the cell viability of 786-O cells, p-coumaric and caffeic acids decreased the renal cancer cells viability. Nevertheless, further studies on the biological activity of these compounds, using other methodologies, as well as using other cancer cell models, should be performed.

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A study of photocatalytic degradation of methomyl and its commercial product Lannate-90

Chemical Industry and Chemical Engineering Quarterly ◽

10.2298/ciceq190424002t ◽

2020 ◽

Vol 26 (3) ◽

pp. 237-247

Author(s):

Andjelka Tomasevic ◽

Aleksandar Marinkovic ◽

Dusan Mijin ◽

Marina Radisic ◽

Slavica Porobic ◽

...

Keyword(s):

Photocatalytic Degradation ◽

High Performance ◽

Diffuse Reflectance Spectroscopy ◽

Degradation Products ◽

Aqueous Suspension ◽

Commercial Product ◽

Photochemical Degradation ◽

Liquid Chromatography Tandem Mass ◽

The Stability ◽

The Impact

Photocatalytic degradation of the carbamate insecticide methomyl and its commercial product Lannate-90 in aqueous suspension of TiO2 and ZnO using an Osram ultra-vitalux' lamp (315-400 nm) was investigated. The objective of the investigation was to study and compare the rates of photochemical degradation of the pure active compound and its formulated product in order to find out the impact of inert ingredients on degradation processes. The photochemical degradation of pure methomyl was found to be slower than the degradation of its commercial product Lannate-90. Photodegradation was investigated by UV-Vis spectrometry and HPLC, while mineralization was monitored using IC and TOC analyses. Light absorption capabilities of catalysts were characterized by ultraviolet-visible diffuse reflectance spectroscopy method, while the stability of catalysts was studied by infrared analyses. Degradation products of methomyl and its formulated product were identified by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) results and tentative photodegradation pathways were proposed.

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Cellular dosimetry of [177Lu]Lu-DOTA-[Tyr3]octreotate radionuclide therapy: the impact of modeling assumptions on the correlation with in vitro cytotoxicity

EJNMMI Physics ◽

10.1186/s40658-020-0276-5 ◽

2020 ◽

Vol 7 (1) ◽

Cited By ~ 1

Author(s):

Giulia Tamborino ◽

Marijke De Saint-Hubert ◽

Lara Struelens ◽

Dayana C. Seoane ◽

Eline A. M. Ruigrok ◽

...

Keyword(s):

Radionuclide Therapy ◽

In Vitro Cytotoxicity ◽

Cellular Dosimetry ◽

The Impact

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Phenolic Composition, Antioxidant Capacity and in vitro Cytotoxicity Assessment of Fruit Wines

Food Technology and Biotechnology ◽

10.17113/ftb.54.02.16.4208 ◽

2016 ◽

Vol 54 (2) ◽

Cited By ~ 19

Author(s):

Ana Ljevar ◽

◽

Natka Ćurko ◽

Marina Tomašević ◽

Kristina Radošević ◽

...

Keyword(s):

Antioxidant Capacity ◽

In Vitro Cytotoxicity ◽

Phenolic Composition ◽

Cytotoxicity Assessment

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In vitro cytotoxicity assessment of carbon fabric coated with calcium phosphate

Carbon ◽

10.1016/j.carbon.2008.06.009 ◽

2008 ◽

Vol 46 (11) ◽

pp. 2-3

Author(s):

Hong-mei Han ◽

Gary J. Phillips ◽

Sergey V. Mikhalovsky ◽

Andrew W. Lloyd

Keyword(s):

Calcium Phosphate ◽

In Vitro Cytotoxicity ◽

Carbon Fabric ◽

Cytotoxicity Assessment

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The cytotoxic activity of pine needles ethanolic extract of Pinus merkusii on HeLa cell lines

BIO Web of Conferences ◽

10.1051/bioconf/20213303001 ◽

2021 ◽

Vol 33 ◽

pp. 03001

Author(s):

Annise Proboningrat ◽

Amaq Fadholly ◽

Sri Agus Sudjarwo ◽

Fedik Abdul Rantam ◽

Agung Budianto Achmad

Keyword(s):

Cytotoxic Activity ◽

Cell Lines ◽

Anticancer Agents ◽

Anticancer Agent ◽

Ethanolic Extract ◽

In Vitro Cytotoxicity ◽

Hela Cell Lines ◽

Cytotoxicity Assessment ◽

Pinus Merkusii

Several efforts have been made to discover new anticancer agents based on natural ingredients. Meanwhile, previous studies have shown that different Pine genus species exhibit cytotoxic activity against various types of cancer cells. This plant is rich in phenolic compounds, especially procyanidins, flavonoids, and phenolic acids. Therefore, this study aims to investigate the in vitro cytotoxicity of Pinus merkusii needles extract on HeLa cancer cell lines. The cytotoxicity assessment was measured using MTT assay and expressed as IC50 value. The results showed that the ethanolic extract poses a dose and time-dependent cytotoxic activity with an IC50 value of 542.5 µg/ml at 48 hours of incubation. Based on this result, Pinus merkusii needles’ ethanolic extract has the potential of a novel candidate for an anticancer agent.

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ANTIOXIDANT, CYTOTOXICITY, AND STABILITY EVALUATION OF GINKGO BILOBA EXTRACT-BASED MICROEMULSIONS FOR ENHANCED THERAPEUTIC ACTIVITY

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i8.19537 ◽

2017 ◽

Vol 10 (8) ◽

pp. 335 ◽

Cited By ~ 1

Author(s):

Manisha Singh ◽

Surya Pratap Singh ◽

Rachana R

Keyword(s):

Ginkgo Biloba ◽

Therapeutic Potential ◽

Ginkgo Biloba Extract ◽

In Vitro Cytotoxicity ◽

Scavenging Activity ◽

Therapeutic Implications ◽

Chromatography Analysis ◽

Ic50 Value ◽

Cytotoxicity Assessment

Objective: This study is aimed to evaluate the antioxidant (AO) potential, cytotoxicity, and stability of preformulated Ginkgo biloba standard extractmicroemulsion (GBME), to investigate if, it retains the therapeutic potential of EGB761 and remains safe and stable for a longer period.Method and Results: GBME has shown enhanced AO (85.2±0.78%, IC50=31.3±0.45 μg/ml) in comparison to EGB761 (74.1±0.51%,IC50=49.4±0.05 μg/ml) using 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assay. Similarly, 2,2-diphenyl-1-picryl-hydrazyl-hydrate(DPPH) assay has also shown that AO for GBME (94.6±0.04%, IC50=11.4±1.03 μg/ml) was higher than EGB761 (78.6±1.20%, IC50=34.6±0.81 μg/ml).Further, IC50 value of antiradical unit of GBME was much lesser (ABTS=14.3±1.05 μg/ml and DPPH=17.03±1.8 μg/ml) in comparison to EGB761(ABTS=34.1±1.62 μg/ml and DPPH=37.5±0.08 μg/ml). Equivalently, both, hydrogen peroxide scavenging activity, and nitric oxide activity wereappreciably higher for GBME than the pure extract. The in vitro cytotoxicity assessment showed that GBME is quite safe (98.68±0.76% cell viability) incomparison to EGB761 (83.29±1.02%). Thereafter, these samples were tested for stability by evaluating their AO activity along with high-performanceliquid chromatography analysis, for the major phytocompounds, after 1 year, and results suggested that AO of GBME remained stable while comparingwith the freshly prepared GBME, whereas AO of EGB761 reduced significantly as compared to freshly taken EGB761 extract implying the degradationof phytocompounds supporting decrease in AO activity.Conclusion: Therefore, the observed results suggest that GBME maintained AO and scavenging activity along with enhanced shelf life with no observedtoxicity, which can be explored further for its potential therapeutic implications in various oxidative stress-induced central nervous system disorders.

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Glucose Degradation Products and Peritoneal Membrane Function

Peritoneal Dialysis International ◽

10.1177/089686080102100218 ◽

2001 ◽

Vol 21 (2) ◽

pp. 201-207 ◽

Cited By ~ 15

Author(s):

Janusz Witowski ◽

Thorsten O. Bender ◽

Gerhard M. Gahl ◽

Ulrich Frei ◽

Achim Jörres

Keyword(s):

Peritoneal Dialysis ◽

Cell Viability ◽

Degradation Products ◽

Membrane Function ◽

Peritoneal Mesothelial Cells ◽

Glucose Degradation Products ◽

And Function ◽

The Impact ◽

Dialysis Fluids

Background The bioincompatibility of peritoneal dialysis fluids (PDF) in current use has been partially attributed to the presence of glucose degradation products (GDPs), which are generated during heat sterilization of PDF. Several of the GDPs have been identified and we have recently demonstrated that these GDPs per se may impair the viability and function of human peritoneal mesothelial cells (HPMC) in vitro. It is also possible that GDP-related toxicity is further exacerbated by the milieu of PDF. We review the current literature on GDP and present the results of experiments comparing the impact of heat- and filter-sterilized PDF on the viability and function of HPMC. Methods Peritoneal dialysis fluids with low (1.5%) and high (4.25%) glucose concentrations were laboratory prepared according to the standard formula and sterilized either by heat (H-PDF; 121°C, 0.2 MPa, 20 minutes) or filtration (F-PDF; 0.2 μ). The buildup of GDP was confirmed by UV absorbance at 284 nm. Confluent HPMC monolayers were exposed to these solutions mixed 1:1 with standard M199 culture medium. After 24 hours, cell viability was assessed with the MTT assay, and interleukin-1β–stimulated monocyte chemotactic protein-1 (MCP-1) release with specific immunoassay. Results Exposure of HPMC to H-PDF resulted in a significant decrease in cell viability, with solutions containing 4.25% glucose being more toxic than 1.5% glucose-based PDF (27.4% ± 3.4% and 53.4% ± 11.0% of control values, respectively). In contrast, viability of HPMC exposed to F-PDF was not different from that of control cells. Moreover, treatment with H-PDF impaired the release of MCP-1 from HPMC to a significantly greater degree compared to F-PDF (17.4% and 24.9% difference for low and high glucose PDF, respectively). Conclusions Exposure of HPMC to H-PDF significantly impairs cell viability and the capacity for generating MCP-1 compared to F-PDF. This effect is likely to be mediated by GDPs present in H-PDF but not in F-PDF.

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The Synthesis, Characterization, Cytotoxic Activity Assessment and Structure–Activity Relationship of 4-Aryl-6-(2,5-dichlorothiophen-3-yl)-2-methoxypyridine-3-carbonitriles

Molecules ◽

10.3390/molecules24224072 ◽

2019 ◽

Vol 24 (22) ◽

pp. 4072

Author(s):

Al-Refai ◽

Ibrahim ◽

Azmi ◽

Osman ◽

Bakar ◽

...

Keyword(s):

Cell Lines ◽

Cancer Cell ◽

Cancer Cell Lines ◽

In Vitro Cytotoxicity ◽

Basic Medium ◽

Condensation Process ◽

Cytotoxicity Activities ◽

Cytotoxicity Assessment ◽

Relationship Of

A series of 2-methoxypyridine-3-carbonitrile (5a–i)-bearing aryl substituents were successfully synthesized in good yields by the condensation of chalcones (4a–i) with malononitrile in basic medium. The condensation process, in most cases, offers a route to a variety of methoxypyridine derivatives (6a–g) as side products in poor yields. All new compounds were fully characterized using different spectroscopic methods. Mass ESI-HMRS measurements were also performed. Furthermore, these compounds were screened for their in vitro cytotoxicity activities against three cancer cell lines; namely, those of the liver (line HepG2), prostate (line DU145) and breast (line MBA-MB-231). The cytotoxicity assessment revealed that compounds 5d, 5g, 5h and 5i exhibit promising antiproliferative effects (IC50 1–5 µM) against those three cancer cell lines.

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Targeting neuropilin-1 suppresses the stability of CD4+CD25+ regulatory T cells via the NF-κB signaling pathway in sepsis

Infection and Immunity ◽

10.1128/iai.00399-20 ◽

2020 ◽

Author(s):

Yu-lei Gao ◽

Chun-xue Wang ◽

Zi-yi Wang ◽

Wen-jie Li ◽

Yan-cun Liu ◽

...

Keyword(s):

T Cells ◽

Regulatory T Cells ◽

Signaling Pathway ◽

Binding Activity ◽

Dna Binding Activity ◽

Sepsis Model ◽

The Stability ◽

The Impact ◽

Tgf Β1

Neuropilin (Nrp)-1 contributes to maintain the stability of CD4+CD25+ regulatory T cells (Tregs). We investigated the impact of Nrp-1 on the stability of CD4+CD25+ Tregs, and the underlying signaling pathways, in a sepsis model. Splenic CD4+CD25+ Tregs were treated with anti-Nrp-1, or transfected to silence Nrp-1 and ikkβ, or administered with PDTC, followed by rSema3A in sepsis simulation. After creation of a sepsis model in mice, anti-Nrp-1 was administered. Expression of foxp3- TSDR, apoptosis rate, Foxp-3/CTLA-4/TGF-β1, IL-10 and TGF-β1, and NF-κB signaling activity of CD4+CD25+ Tregs were determined. Sepsis simulation with or without rSema3A increased the stability of CD4+CD25+ Tregs, including an increase in the expression of Foxp-3/CTLA-4/TGF-β1, decrease in apoptosis and methylation of foxp3- TSDR, increase in the secretion of TGF-β1 and IL-10, and increase in the immunosuppressive effect on CD4+T lymphocytes. silencing of Nrp-1 or anti-Nrp-1 treatment interdicted LPS stimulation with or without a rSema3A-mediated effect. Sepsis simulation increased the DNA-binding activity of NF-κB, as well as the p-ikkβ/ikkβ and p-P65/P65 ratios in vitro and vivo. Silencing of ikkβ expression or PDTC treatment suppressed the stability of CD4+CD25+ Tregs in LPS-induced sepsis. Weakening Nrp-1 reduced the stability of CD4+CD25+ Tregs by regulating the NF-κB signaling pathway, and could be a new target for immunoregulation in sepsis.

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