Molecular diagnosis of Salmonella typhi and its virulence in suspected typhoid blood samples through nested multiplex PCR

2017 ◽  
Vol 139 ◽  
pp. 150-154 ◽  
Author(s):  
Solai Ramatchandirane Prabagaran ◽  
Vellingiri Kalaiselvi ◽  
Naganathan Chandramouleeswaran ◽  
Krishnan Nair Geetha Deepthi ◽  
Kootallur Narayanan Brahmadathan ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Molecular Diagnosis ◽  
Salmonella Typhi ◽  
Blood Samples

scholarly journals Detection of Salmonella typhi Using Multiplex and Monoplex PCR in Tifoid Fever Patients

2019 ◽  
Author(s):  
Andika Aliviameita ◽  
Meryn Linanda Agustin ◽  
Anggie Wedha Puspita ◽  
Miftahul Mushlih ◽  
Puspitasari Puspitasari ◽  
...  
Keyword(s):  
Typhoid Fever ◽  
Multiplex Pcr ◽  
Human Blood ◽  
Rapid Detection ◽  
Detection Method ◽  
Salmonella Typhi ◽  
Molecular Methods ◽  
Blood Samples ◽  
Global Problem ◽  
Human Blood Samples

Typhoid fever is a disease caused by Salmonella typhi infection. This disease is still a global problem. The purpose of this study was to develop a rapid detection method for Salmonella using molecular methods utilizing Inv A and Fim-C Genes. Two methods compared, which are Multiplex and monoplex PCR. The sample is in the form of the patient's blood, which is stored at 3 ml EDTA vacutainer tube consisting of 10 positive samples and five negative samples. The criteria for samples used previously have tested by widal/ immunoserology (titer more than 1/160). The results showed that multiplex PCR for detection of Salmonella using the Inv A gene and fim c gene not recommended because too many bands produced. The application of monoplex in the Inv A gene gives better results than the Fim C gene. In conclusion, the monoplex application on Inv A gene recommended than Fim C gene used to detect S. typhi in human blood samples.

scholarly journals Multiplex PCR for the concurrent detection and differentiation of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium

2011 ◽  
Vol 39 (1) ◽  
pp. 9-15 ◽  
Author(s):  
Chai Fung Pui ◽  
Woan Chwen Wong ◽  
Lay Ching Chai ◽  
Hai Yen Lee ◽  
Ahmad Noorlis ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Multiplex Pcr ◽  
Salmonella Typhi ◽  
Salmonella Spp

scholarly journals Bacterial aerobic Bacteraemia at AL- Kindi Teaching Hospital 2007-2009: Etiology diversity, Clinical features, and Outcome.

2012 ◽  
Vol 6 (1) ◽  
pp. 3-9
Author(s):  
Suzan I. Majeed ◽  
Khawlah J. Khalaf ◽  
Mazin K. Ameen
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Clinical Features ◽  
Teaching Hospital ◽  
Blood Stream ◽  
Salmonella Typhi ◽  
Blood Stream Infections ◽  
Blood Samples

Data regarding the incidence, etiology and outcome of Bacteraemia taken from AL-Kindi hospital were collected, and compared the situation between the three years: 2007, 2008, 2009. A total number of blood samples was (505), from this number only (70) (13.8%) is positive. The rate of positivity was significant greater in 2009 (17.7%) than 2008 (11.8%) and 2007 (9.1%). The calculated incidence of significant episodes of bacteraemia was 12.6% .The five most commonly isolated microorganisms were: Salmonella typhi30(46.8%), Staphylococcus aureus 12 (18.7%), Escherichia coli 10 (15.6%),Klebsiella 4(6.2%) and Enterobacter sp.3(4.6%) varied throughout this period, significant increase was noted in 2009. The acquisition of blood stream infections was nosocomial in 8.5% of cases.60% of Salmonella typhi were Amoxicillin resistant. Also Staph.aureus isolates, 58%were resistant to Amoxicillin and Erythromycin. Cephalexin-resistant E.coli in70% of the isolates and Gentamycin-resistant Klebsiella occurred in 75% of the isolates. The incidence of BSI did not differ significantly from other reported studies.

scholarly journals 1439. High Rates of Drug Resistance in Escherichia coli from a Pilot Antimicrobial Resistance Surveillance System in Cambodia

2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S723-S724
Author(s):  
Vandarith Nov ◽  
Darapheak Chau ◽  
Chhorvann Chhea
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Blood Culture ◽  
Klebsiella Pneumoniae ◽  
Surveillance System ◽  
Methicillin Resistance ◽  
Salmonella Typhi ◽  
Blood Samples

Abstract Background Antimicrobial resistance (AMR) is a major and growing global public health problem. The Cambodia Ministry of Health established a pilot laboratory-based AMR surveillance system for blood specimens in 2017. The objective of this study is to characterize AMR among pathogenic isolates from blood samples. Methods A retrospective analysis was performed using one year of data from a pilot AMR Surveillance system in Cambodia. Four blood culture isolate pathogens were included: Escherichia coli, Klebsiella pneumoniae, Salmonella Typhi /Salmonella Paratyphi A and Staphylococcus aureus. Blood culture isolates that were referred from eleven sentinel sites were analyzed at the National Public Health Laboratory for identification. Antibiotic susceptibility testing (AST) was done using disk diffusion, minimum inhibitory concentration method following Clinical Laboratory Standard Institute (CLSI) guidelines. Results Among 214 pathogenic isolates from blood samples, E.coli was the most common (56.1%), followed by Salmonella Typhi/Salmonella Paratyphi A (18.7%), Staphylococcus aureus (13.5%), and Klebsiella pneumoniae (11.7%). Methicillin Resistance Staphylococcus aureus (MRSA) was detected in half of the isolates. E.coli was resistant to ampicillin (94.4%), trimethoprim-sulfamethoxazole (84.5%), and ceftriaxone (79.2%). Salmonella Typhi was resistant to ampicillin (73.3%) and trimethoprim-sulfamethoxazole (60.0%) and Salmonella Paratyphi A were resistant to fluoroquinolones (91.7%). For last resort antibiotics, E.coli was resistant to carbapenem groups (3.2% for imipenem, 4.9% for meropenem, and 5.0% for ertapenem). Klebsiella pneumoniae was not resistant to any groups. Conclusion E.coli was found at high rates in blood samples and was resistant to common antibiotics used in Cambodia. These pilot data show the importance of establishing a national AMR surveillance system in Cambodia to monitor AMR trends following GLASS guidelines. Disclosures All Authors: No reported disclosures

scholarly journals Molecular identification by multiplex polymerase chain reaction of Pasteurella multocida in cattle and buffaloes in Baghdad

2014 ◽  
Vol 38 (1) ◽  
pp. 99-106
Author(s):  
Ihab G. M. AL-Shemmari
Keyword(s):  
Polymerase Chain Reaction ◽  
Multiplex Pcr ◽  
Multiplex Polymerase Chain Reaction ◽  
Pasteurella Multocida ◽  
Type B ◽  
Chain Reaction ◽  
Blood Samples ◽  
Nasal Swabs ◽  
Polymerase Chain ◽  
B 31

The aim of this study was to identify pasteurella multocida and their types by PCR in cattle’s and buffaloesi bagdad from March to August 2012 on 204 animals , including 102 cattle and 102 buffaloes at slaughter houses from Baghdad .Blood samples and nasal swaps were collected , before slaughtering and lung tissues of slaughtered animal , and from 54 clinically suspected cases of pasteurellosis , including 27 bovines ,and 27 buffaloes the samples taken included blood and nasal swabs . Pasteurellamultocida were isolated from 94 animals include 49 cattle 45 buffaloes. The typing of the isolates by multiplex PCR for genotyping Pasteuerllamultocida revealed 93 isolates of type B , 31 from cattle and 62 from buffaloes ,and 81 isolates of type A , 55 from cattle and 26 from buffaloes .

scholarly journals Laboratory Based Diagnosis of Bacteraemia among Inpatients and Outpatients with Acute Febrile Illness at Khwaja Yunus Ali Medical College and Hospital in Bangladesh

2020 ◽  
pp. 46-51 ◽  
Keyword(s):  
Antibiotic Susceptibility ◽  
Tertiary Care ◽  
Salmonella Typhi ◽  
Susceptibility Test ◽  
Gram Positive ◽  
Blood Samples ◽  
E Coli ◽  
Antibiotic Susceptibility Test ◽  
Acinetobacter Spp ◽  
Gram Positive Cocci

Bacteraemia is one of the important causes of mortality and morbidity worldwide. The emergence of multidrug-resistant bacterial strains is a major problem in its management. To encourage the prudent use of appropriate antibiotics in a tertiary care study was undertaken. A total of 282 patients with suspected bacteraemia from indoor and outdoor patients were included in this study. Blood samples were processed in the microbiology laboratory and bacteria were recognized by standard laboratory methods and then antibiotic susceptibility test was performed following CLSI guidelines. Out of a total of 282 blood samples, 55(19.50%) were culture positive. Among 55 positive cultures, Gram-positive Cocci and Gram-negative Bacilli were 48(87.3%) and 7(12.7%) respectively. The most predominant organisms were Staphylococcus aureus comprising 42(76.36%), followed by Salmonella typhi 4(7.30%), E. coli 2(3.63%) and Acinetobacter spp 1(1.81%). Among the isolated Staphylococcus spp, only 6(10.9%) isolates were Coagulase-Negative (CoNS). In antibiotic susceptibility test, all of the isolated bacterial pathogens were found susceptible to imipenem and meropenem (100%). For Salmonella typhi, all 4 isolated strains were found resistant to amoxicillin, amoxicillin+clavulanic acid (amoxyclave) and cephradine and 3 out of 4(75%) were susceptible to ciprofloxacin and levofloxacin. Most of the Gram-positive Cocci (GPC) were susceptible to levofloxacin (90%), ciprofloxacin (85%), and amikacin (83%). Both of E. coli and Acinetobacter spp were sensitive to amikacin (100%). This study stresses the need for continuous screening and surveillance for antibiotic resistance that would influence appropriate empiric treatment and infection control strategies for bacteremic cases.

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