Effectiveness of Honey on S.aureus and P.aeruginosa Biofilms

2008 ◽  
Vol 139 (2_suppl) ◽  
pp. P107-P107 ◽  
Author(s):  
Talal Alandejani ◽  
Joseph G Marsan ◽  
Wendy Ferris ◽  
Slinger Robert ◽  
Frank Chan
Keyword(s):  
Bacterial Resistance ◽  
Bacterial Biofilms ◽  
Bacterial Isolates ◽  
Manuka Honey ◽  
Antibiotic Resistant ◽  
New Approach ◽  
Biofilm Model ◽  
Planktonic Form ◽  
And Staphylococcus Aureus ◽  
Topical Antimicrobial

Problem Biofilms formed by Pseudomonas aeruginosa (PA) and Staphylococcus aureus (SA) have been shown to be an important factor in the pathophysiology of chronic rhinosinusitis (CRS). Growth in biofilms increases bacterial resistance to antibiotics, which may explain why CRS responds poorly to antibiotic therapy. Honey has been used as an effective topical antimicrobial agent in the treatment of antibiotic-resistant bacterial wound infections. To our knowledge, no research has studied the effect of honey on biofilms. Methods We used a previously established biofilm model to assess antibacterial activity of Manuka honey from New Zealand and Sidr honey from Yemen. These were tested at a 1 in 2 dilution against 11 isolates of methicillin-susceptible SA (MSSA), 11 methicillin-resistant SA (MRSA), and 11 PA isolates. Honeys were tested against both planktonic (broth) and biofilm-grown bacteria. Results Both honeys were effective in killing 100% of the isolates in the planktonic form. Biofilm-grown bacteria were less susceptible to the honeys, but honey still had significant bactericidal activity. The bactericidal rate for the Sidr and Manuka honeys against MSSA MRSA and PA were 63%, 73%, 91% and 82%, 63% and 91%, respectively. These rates were significantly higher (p<0.001) than those seen with single antibiotics commonly used against MSSA and MRSA (e.g. cloxacillin and vancomycin) in a previous study done on the same bacterial isolates. Conclusion Honey, which is a natural, non-toxic and inexpensive product, is effective in killing SA and PA bacterial biofilms. Significance This intriguing observation may have important clinical implications and could lead to a new approach for treating refractory CRS.

scholarly journals Chlorine disinfection against Legionella pneumophila biofilms

2020 ◽  
Vol 10 (4) ◽  
pp. 885-893
Author(s):  
Abdelwahid Assaidi ◽  
Mostafa Ellouali ◽  
Hassan Latrache ◽  
Hafida Zahir ◽  
Abdelaziz Karoumi ◽  
...  
Keyword(s):  
Legionella Pneumophila ◽  
Bacterial Resistance ◽  
Galvanized Steel ◽  
Opportunistic Pathogens ◽  
New Approach ◽  
Force Microscopy ◽  
Chlorine Disinfection ◽  
Atomic Force ◽  
Premise Plumbing ◽  
Planktonic Form

Abstract Premise plumbing promotes the regrowth and survival of opportunistic pathogens, such as Legionella pneumophila (L. pneumophila), especially within biofilms. The purpose of this study was to investigate the effectiveness of chlorine disinfection against L. pneumophila serogroup 1 and serogroup 2–15 planktonic form and biofilms. Planktonic cells were able to survive during the study period in the presence of chlorine at recommended free chlorine levels (0.2–0.5 mg/L). Biofilms were developed on galvanized steel and polyvinyl chloride (PVC) for 18 and 30 days and exposed to 50, 100, 150, and 200 mg/L for 2 hours. No colony appeared immediately after chlorination; however, persistent cells were able to tolerate treatment and continue to grow on subsequent days. The biofilm formation was evaluated by atomic force microscopy. This study demonstrates that the biofilm formed on the surfaces of plumbing materials increases bacterial resistance against high levels of chlorination. A new approach towards monitoring and eradicating L. pneumophila from water systems is required.

scholarly journals Surface-bound reactive oxygen species generating nanozymes for selective antibacterial action

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Feng Gao ◽  
Tianyi Shao ◽  
Yunpeng Yu ◽  
Yujie Xiong ◽  
Lihua Yang
Keyword(s):  
Reactive Oxygen Species ◽  
Mammalian Cells ◽  
Bacterial Resistance ◽  
Reactive Oxygen ◽  
Resistant Bacteria ◽  
Oxygen Species ◽  
Antibiotic Resistant ◽  
Generate Surface ◽  
Inert Substrate ◽  
Silver Palladium

AbstractActing by producing reactive oxygen species (ROS) in situ, nanozymes are promising as antimicrobials. ROS’ intrinsic inability to distinguish bacteria from mammalian cells, however, deprives nanozymes of the selectivity necessary for an ideal antimicrobial. Here we report that nanozymes that generate surface-bound ROS selectively kill bacteria over mammalian cells. This result is robust across three distinct nanozymes that universally generate surface-bound ROS, with an oxidase-like silver-palladium bimetallic alloy nanocage, AgPd0.38, being the lead model. The selectivity is attributable to both the surface-bound nature of ROS these nanozymes generate and an unexpected antidote role of endocytosis. Though surface-bound, the ROS on AgPd0.38 efficiently eliminated antibiotic-resistant bacteria and effectively delayed the onset of bacterial resistance emergence. When used as coating additives, AgPd0.38 enabled an inert substrate to inhibit biofilm formation and suppress infection-related immune responses in mouse models. This work opens an avenue toward biocompatible nanozymes and may have implication in our fight against antimicrobial resistance.

ISOLASI DAN SKRINING AKTIVITAS ANTIBAKTERI DARI BAKTERI SIMBION NUDIBRANCHIA Phyllidiella pustulosa dan Thuridilla lineolata

2020 ◽  
Vol 8 (2) ◽  
pp. 40
Author(s):  
Ade Kurniawan Arie ◽  
Rosita A J Lintang ◽  
Remy E P Mangindaan ◽  
Agung B Windarto ◽  
Fitje Losung ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Symbiotic Bacteria ◽  
Bacterial Isolates ◽  
Defense Systems ◽  
Self Defense ◽  
Solid Media ◽  
Antibacterial Assay ◽  
And Staphylococcus Aureus

Nudibranchs are marine invetebrates belongs to the class of Gastropoda that are able to camouflage and develop a self-defense systems. Marine bacteria contain chemicals compunds that have potentials on marine drugs discovery through the secondary metabolism. The purpose of this study was to isolate the symbiotic bacteria from nudibranches Phylidiella pustulosa and Thuridilla lineolate and to screen the antibacterial activity of these bacterial isolates against Escherichia coli and Staphylococcus aureus. Isolation and culture of the symbiotic bacteria was made on B1 (solid) media. The results of the antibacterial assay showed that the symbiotic bacterial isolates from Phylidiella pustulosa and Thuridilla lineolate were able to inhibit the growth of the Staphylococcus aureus and Escherichia coli test. Keywords: nudibranch, Phyllidiella pustulosa, Thuridilla lineolate, bacteria, antibacterialAbstrakNudibranch adalah avetebrata laut dalam kelas Gastropoda yang mampu melakukan kamuflase dan mengembangkan sistem pertahanan diri. Bakteri laut mengandung senyawa kimia yang berpotensi sebagai bahan obat-obatan yang dihasilkan melalui metabolit sekunder. Tujuan dari penelitian ini yaitu untuk mengisolasi bakteri yang bersimbion dengan nudibranch Phylidiella pustulosa dan Thuridilla lineolate, kemudian mengamati aktivitas antibakteri dari isolat bakteri tersebut terhadap Escherichia coli danStaphylococcus aureus. Isolasi dan kultur bakteri yang bersimbion dibuat pada media B1 (padat). Skrining aktivitas antibakteri menunjukkan isolat bakteri yang bersimbion dengan nudibranch Phylidiella pustulosa dan Thuridilla lineolate mampu menghambat pertumbuhan organisme uji Staphylococcus aureus dan Escherichia coli. Kata kunci: nudibranch, Phyllidiella pustulosa, Thuridilla lineolate, bakteri, antibakteri

A Microbiologic Approach to the Investigation of Bacterial Nosocomial Infection Outbreaks

1980 ◽  
Vol 1 (06) ◽  
pp. 391-400 ◽  
Author(s):  
Donald A. Goldmann ◽  
Ann B. Macone
Keyword(s):  
Nosocomial Infection ◽  
Susceptibility Testing ◽  
Resistant Bacteria ◽  
Antibiotic Susceptibility Testing ◽  
Bacterial Isolates ◽  
Infection Control Team ◽  
Antibiotic Resistant ◽  
Plasmid Analysis ◽  
Identification Of Bacteria ◽  
Bacteriocin Typing

This article details the appropriate microbiologic support that is critical to the successful investigation of nosocomial infection problems. The infection control team must have ready access to microbiologic data, and the laboratory should retain epidemiologically relevant bacterial isolates. Investigation of epidemics is facilitated by precise identification of bacteria and careful antibiotic susceptibility testing. In some situations, biotyping, serotyping, phage typing, bacteriocin typing, and other specialized techniques may be required. Plasmid analysis may be useful in the investigation of nosocomial infection problems caused by antibiotic-resistant bacteria.

scholarly journals Absence of bacterial resistance to medical-grade manuka honey

2010 ◽  
Vol 29 (10) ◽  
pp. 1237-1241 ◽  
Author(s):  
R. A. Cooper ◽  
L. Jenkins ◽  
A. F. M. Henriques ◽  
R. S. Duggan ◽  
N. F. Burton
Keyword(s):  
Bacterial Resistance ◽  
Manuka Honey ◽  
Medical Grade

scholarly journals Detection of pathogenic bacteria associated with earphones used by students of Stamford University Bangladesh

2020 ◽  
Vol 10 (1) ◽  
pp. 1-4
Author(s):  
Omor Ahmed Chowdhury ◽  
Md Raihan Ahmed ◽  
Md Raihan Dipu ◽  
Md Aftab Uddin
Keyword(s):  
Pathogenic Bacteria ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
Disk Diffusion Method ◽  
Antibiotic Resistant ◽  
Potential Source ◽  
Different Types ◽  
Staphylococcus Spp

The use of earphones has increased in recent times throughout the world especially among the different level of students such as school, college or university who have a higher tendency of sharing these among them. Unlike airline headsets, headphones and stethoscope ear-pieces, ear phones are often shared by multiple users and can be a potential medium for transmission of pathogens, which can give rise to various ear related infections. The objective of this study was to detect the pathogenic bacteria from the ear-phones used by the students of Stamford University Bangladesh. A total of 16 ear-phone swabs were collected by sterile cotton swabs. The swabs were inoculated onto blood agar and incubated aerobically overnight at 37oC. Microscopic observation and standard biochemical tests were performed to confirm the identification of all the bacterial isolates. Six presumptively identified Staphylococcus spp. (38%) were tested against six different types of antibiotics following Kirby-Bauer disk diffusion method. Isolates were found to be 84% resistant against Cotrimoxazole and demonstrated 100% sensitivity to Vancomycin and Ciprorofloxacin. The findings of this study suggest the users to disinfect their respective ear phones and not to exchange them as they may act as a potential source to transfer pathogenic and antibiotic resistant bacteria among the ear phone users. Stamford Journal of Microbiology, Vol.10 (1) 2020: 1-4

scholarly journals Paradigm Shift in Antimicrobial Resistance Pattern of Bacterial Isolates during the COVID-19 Pandemic

Antibiotics ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 954
Author(s):  
Vikas Saini ◽  
Charu Jain ◽  
Narendra Pal Singh ◽  
Ahmad Alsulimani ◽  
Chhavi Gupta ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Tertiary Care ◽  
Public Health Problem ◽  
Resistance Pattern ◽  
Coagulase Negative Staphylococcus ◽  
Care Hospital ◽  
Bacterial Isolates ◽  
Hospital Infection Control ◽  
And Staphylococcus Aureus

Antimicrobial resistance (AMR) is an emerging public health problem in modern times and the current COVID-19 pandemic has further exaggerated this problem. Due to bacterial co-infection in COVID-19 cases, an irrational consumption of antibiotics has occurred during the pandemic. This study aimed to observe the COVID-19 patients hospitalized from 1 March 2019 to 31 December 2020 and to evaluate the AMR pattern of bacterial agents isolated. This was a single-center study comprising 494 bacterial isolates (blood and urine) that were obtained from patients with SARS-CoV-2 admitted to the ICU and investigated in the Department of Microbiology of a tertiary care hospital in Delhi, India. Out of the total bacterial isolates, 55.46% were gram negative and 44.53% were gram positive pathogens. Of the blood samples processed, the most common isolates were CoNS (Coagulase Negative Staphylococcus) and Staphylococcus aureus. Amongst the urinary isolates, most common pathogens were Escherichia coli and Staphylococcus aureus. A total of 60% MRSA was observed in urine and blood isolates. Up to 40% increase in AMR was observed amongst these isolates obtained during COVID-19 period compared to pre-COVID-19 times. The overuse of antibiotics gave abundant opportunity for the bacterial pathogens to gradually develop mechanisms and to acquire resistance. Since the dynamics of SARS-COV-2 are unpredictable, a compromise on hospital antibiotic policy may ultimately escalate the burden of drug resistant pathogens in hospitals. A shortage of trained staff during COVID-19 pandemic renders it impossible to maintain these records in places where the entire hospital staff is struggling to save lives. This study highlights the extensive rise in the use of antibiotics for respiratory illness due to COVID-19 compared to antibiotic use prior to COVID-19 in ICUs. The regular prescription audit followed by a constant surveillance of hospital infection control practices by the dedicated teams and training of clinicians can improve the quality of medications in the long run and help to fight the menace of AMR.

scholarly journals ISOLASI BAKTERI YANG BERSIMBION DENGAN ASCIDIAN Herdmania momus YANG MEMILIKI AKTIVITAS ANTIBAKTERI

2020 ◽  
Vol 8 (2) ◽  
pp. 20
Author(s):  
Silvia E Koyongian ◽  
Deiske A Sumilat ◽  
Rosita A J Lintang ◽  
Stenly Wullur ◽  
Sandra O Tilaar ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Marine Invertebrates ◽  
Nutrient Agar ◽  
Symbiotic Bacteria ◽  
Bacterial Isolates ◽  
Marine Broth ◽  
Antibacterial Screening ◽  
Coral Reef Ecosystems ◽  
And Staphylococcus Aureus

 Ascidian is marine invertebrates in coral reef ecosystems that produce many bioactive compounds for pharmacology. The presence of symbiotic bacteria with marine organisms is protected the host biota by producing secondary metabolites. The purpose of this study is to obtain symbiotic bacterial isolates with Herdmania momus ascidian, then to observe the antibacterial activity of these bacterial isolates against Escherichia coli, and Staphylococcus aureus. Isolation and culture of the symbiotic bacteria were made on Nutrient Agar and Zobell Marine Broth media. The antibacterial screening showed that the Herdmania momus symbiotic bacteria were able to inhibit the growth of Staphylococcus aureus and Escherichia coli.Keywords: ascidians, Herdmania momus, bacteria, isolation, antibacterialAbstak          Ascidian adalah avetebrata laut di ekosistem terumbu karang yang banyak menghasilkan senyawa bioaktif untuk bidang farmakologi. Keberadaan bakteri yang bersimbion dengan organisme laut pada umumnya untuk melindungi biota yang ditumpanginya dan dirinya dengan cara menghasilkan senyawa metabolit sekunder. Tujuan dari penelitian ini yaitu untuk mendapatkan isolat bakteri yang bersimbion dengan ascidian Herdmania momus, kemudian mengamati aktivitas antibakteri dari isolat bakteri tersebut terhadap Escherichia coli, dan Staphylococcus aureus. Isolasi dan kultur bakteri yang bersimbion dengan ascidian dibuat pada media Nutrient Agar dan Zobell Marine Broth. Skrining aktivitas antibakteri menunjukkan isolat bakteri yang bersimbion dengan ascidian Herdmania momus mampu menghambat pertumbuhan organisme uji Staphylococcus aureus dan Escherichia coli.Kata kunci: ascidian, Herdmania momus, bakteri, isolasi, antibakteri

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