Antigenotoxic effects of ferulic acid on sepsis-induced DNA damage in the liver and kidney of Wistar albino rats

Reactive oxygen species are believed to be involved in the development of sepsis. Plant-derived phenolic compounds are thought to be possible therapeutic agents against sepsis because of their antioxidant properties. Rosmarinic acid (RA) is a phenolic compound commonly found in various plants, which has many biological activities including antioxidant activity. The aim of this study was to investigate the effects of RA on sepsis-induced DNA damage in the lymphocytes and liver and kidney cells of Wistar albino rats by alkaline comet assay with and without formamidopyrimidine DNA glycosylase protein. The oxidative stress parameters such as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities and total glutathione (GSH) and malondialdehyde (MDA) levels in the liver and kidney tissues and an inflammatory cytokine, tumor necrosis factor α (TNF-α) level in plasma were also evaluated. It is found that DNA damage in the lymphocytes, livers, and kidneys of the RA-treated rats was significantly lower than that in the sepsis-induced rats. RA treatment also decreased the MDA levels and increased the GSH levels and SOD and GSH-Px activities in the livers and kidneys of the sepsis-induced rats. Plasma TNF-α level was found to be decreased in the RA-treated rats. It seems that RA might have a role in the attenuation of sepsis-induced oxidative damage not only by decreasing the DNA damage but also by increasing the antioxidant status and DNA repair capacity of the animals.

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The protective role of ferulic acid on sepsis-induced oxidative damage in Wistar albino rats

Environmental Toxicology and Pharmacology ◽

10.1016/j.etap.2014.08.018 ◽

2014 ◽

Vol 38 (3) ◽

pp. 774-782 ◽

Cited By ~ 26

Author(s):

Merve Bacanlı ◽

Sevtap Aydın ◽

Gökçe Taner ◽

Hatice Gül Göktaş ◽

Tolga Şahin ◽

...

Keyword(s):

Oxidative Damage ◽

Ferulic Acid ◽

Protective Role ◽

Albino Rats ◽

Wistar Albino Rats

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Protective effects of rosmarinic acid on sepsis-induced DNA damage in the liver of Wistar albino rats

Frontiers in Genetics ◽

10.3389/conf.fgene.2015.01.00020 ◽

2015 ◽

Vol 6 ◽

Author(s):

Goktas Hatice ◽

Bacanli Merve ◽

Aydin Sevtap ◽

Taner Gokce ◽

Sahin Tolga ◽

...

Keyword(s):

Dna Damage ◽

Rosmarinic Acid ◽

Albino Rats ◽

Protective Effects ◽

Wistar Albino Rats

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Antihyperlipidemic and Biochemical Activities of Mcy Protein in Streptozotocin Induced Diabetic Rats

Cellular Physiology and Biochemistry ◽

10.1159/000373954 ◽

2015 ◽

Vol 35 (4) ◽

pp. 1326-1334 ◽

Cited By ~ 7

Author(s):

Saritha Marella ◽

Dilip Rajasekhar Maddirela ◽

Kameswara Rao Badri ◽

Malaka Venkateshwarulu Jyothi Kumar ◽

Apparao Chippada

Keyword(s):

Blood Glucose ◽

Fasting Blood Glucose ◽

Diabetic Rats ◽

Albino Rats ◽

Protective Effects ◽

Tissue Lipid ◽

Lipid Levels ◽

Liver And Kidney ◽

Streptozotocin Induced Diabetes ◽

Wistar Albino Rats

Background: This study was aimed to evaluate the protective effects of a novel anti-hyperglycemic “Mcy protein” isolated from the fruits of Momordica cymbalaria in streptozotocin induced- diabetes rat model. Materials and Methods: Wild type and Streptozotocin induced diabetic male wistar albino rats were either treated with single intraperitoneal injection of 2.5 mg Mcy protein/kg body weight or acetate buffer daily for 30 days. Fasting blood glucose and, serum and tissue lipid levels were measured along with biochemical analysis for hepatic and renal function tests. Results: Mcy protein significantly reduced the fasting blood glucose and, serum as well as tissue lipid levels (p<0.05), besides normalizing the levels of liver and kidney function markers in the treated diabetic rats when compared to the diabetic controls. Our studies also showed the pancreatic islet regeneration in Mcy treated rats. Conclusion: Mcy protein can alleviate hyperlipidemia and help manage diabetes by stimulating insulin secretion without evident toxic effects on liver and kidney.

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Ethanolic Extract of Syzygium cumini Causes Toxic Effects on Ethanol-induced Liver and Kidney Damage in Albino Wistar Rats: A Biochemical and Histological Study

Iranian Jornal of Toxicology ◽

10.32598/ijt.16.1.879.1 ◽

2022 ◽

Vol 16 (1) ◽

pp. 63-72

Author(s):

Abba Aji Manu ◽

◽

Bello Muhammad Musa ◽

Martha Orendu Oche Attah ◽

Helga Ishaya Bedan ◽

...

Keyword(s):

Histological Study ◽

Kidney Tissue ◽

Ethanolic Extract ◽

Albino Rats ◽

Syzygium Cumini ◽

Tissue Samples ◽

Inflammatory Conditions ◽

Therapeutic Value ◽

Liver And Kidney ◽

Wistar Albino Rats

Background: The therapeutic value of Syzygium cumini (S. cumini) has been documented in traditional medicine for the treatment of many diseases and ailments. Various preparations of this plant have been made and used especially for liver inflammatory conditions in livestock. Further, many liver diseases in humans are inflammatory conditions, which are caused by alcohol intake. This study sought to examine the effect of S. cumini on ethanol-induced hepatotoxicity in Wistar albino rats. Methods: Twenty-five rats were divided into five groups of five rats each. The first group was control and the other four were administered ethanol at varying doses to induce liver and kidney damages. Two doses of the S. cumini extract were administered at a concentration of 200 mg/kg or 400 mg/kg. Silymarin was administered to the last group at 10 mg/kg. The liver and kidney tissue samples were collected and preserved for histological analyses and the rat sera were analyzed for the associated biochemical biomarkers. Results: Histopathological analyses revealed pyknotic nuclei and distortion in the arrangement of the hepatocytes in extract-treated groups. The kidney tissue samples showed signs of interstitial bleeding and aggregation of lymphocytes in the peri-glomerular areas. The analyses of the biochemical parameters revealed that there were significant increases in the aspartate aminotransferase (AST), alanine transaminase (ALT), Urea and creatinine in the sera of the groups treated with the extract compared to those of the controls (P<0.05). Conclusion: The S. cumini extract caused elevation of serum hepatic and renal biomarkers at 400 mg/kg and did not have a hepatoprotective effect.

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Protective Effects of Pinus halepensis L. Essential Oil on Aspirin-induced Acute Liver and Kidney Damage in Female Wistar Albino Rats

Journal of Oleo Science ◽

10.5650/jos.ess15287 ◽

2016 ◽

Vol 65 (8) ◽

pp. 701-712 ◽

Cited By ~ 6

Author(s):

Hafsia Bouzenna ◽

Noura Samout ◽

Etaya Amani ◽

Sakhria Mbarki ◽

Zied Tlili ◽

...

Keyword(s):

Essential Oil ◽

Pinus Halepensis ◽

Kidney Damage ◽

Albino Rats ◽

Protective Effects ◽

Liver And Kidney ◽

Wistar Albino Rats ◽

Liver And Kidney Damage

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Effects of ferulic acid on oxidative stress parameters in livers and kidneys of Wistar albino rats

Toxicology Letters ◽

10.1016/j.toxlet.2014.06.812 ◽

2014 ◽

Vol 229 ◽

pp. S243-S244

Author(s):

Merve Bacanli ◽

Sevtap Aydin ◽

Gökce Taner ◽

Hatice Gül Göktas ◽

Tolga Sahin ◽

...

Keyword(s):

Oxidative Stress ◽

Ferulic Acid ◽

Albino Rats ◽

Oxidative Stress Parameters ◽

Wistar Albino Rats ◽

Stress Parameters

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Effect of combinations of four trace elements on cadmium bioaccumulation in a few tissues of male albino rats

Journal of Applied and Natural Science ◽

10.31018/jans.v2i1.97 ◽

2010 ◽

Vol 2 (1) ◽

pp. 66-69

Author(s):

A. Usha Rani ◽

D. Kumar Babu ◽

J. Obaiah

Keyword(s):

Trace Elements ◽

Body Weight ◽

Rat Kidney ◽

Albino Rats ◽

Time Intervals ◽

Cd Accumulation ◽

Maximum Decrease ◽

Liver And Kidney ◽

Cadmium Bioaccumulation ◽

Wistar Albino Rats

The present study is designed to investigate the accumulation of cadmium (Cd) in selected tissues such as liver, kidney, testis and small intestine of male albino rats treated for Cd and after combined supplementation with Zinc (Zn) + Iron (Fe) and Selenium (Se) + Copper (Cu). The wistar albino rats were treated with cadmium chloride(CdCl2)at a dose of 1/10th LD50 i.e. 22.5 mg / kg body weight for 7, 15 and 30 days(d) time intervals. After 15 d to Cd treatment, the rats were then supplemented with the above combination of two trace elements and thenobserved for accumulation of Cd at specific time intervals. These trace elements at a dosage of 1 mg/ kg body weight of Se, 16mg/kg body weight of Cu, 12 mg / kg body weight of Zn and 40 mg / kg body weight of Fe were given as supplements. There was significant Cd accumulation in liver and kidney among the selected tissues before to supplementation and there was significant decrease in the Cd accumulation levels in all the tissues after trace element supplementation. Moreover the 30d Zn + Fe supplemented rat kidney showed maximum decrease in Cd accumulation (8.327μg/g wet wt. of the tissue).

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Comparative assessment on the probable mechanisms underlying the hepatorenal toxicity of commercial imidacloprid and hexaflumuron formulations in rats

Environmental Science and Pollution Research ◽

10.1007/s11356-021-18486-z ◽

2022 ◽

Author(s):

Eman I. Hassanen ◽

Ahmed M. Hussien ◽

Sally Mehanna ◽

Marwa A. Ibrahim ◽

Neven H. Hassan

Keyword(s):

Kidney Tissue ◽

Serum Levels ◽

Saline Group ◽

Control Group ◽

Albino Rats ◽

Apoptosis Pathway ◽

Liver And Kidney ◽

Group 2 ◽

Wistar Albino Rats ◽

Tissue Specimens

Abstract Pesticides are viewed as a major wellspring of ecological contamination and causing serious risky consequences for people and animals. Imidacloprid (IM) and hexaflumuron (HFM) are extensively utilized insect poisons for crop assurance on the planet. A few investigations examined IM harmfulness in rodents, but its exact mechanism hasn’t been mentioned previously as well as the toxicity of HFM doesn’t elucidate yet. For this reason, the present study was designed to explore the mechanism of each IM and HFM–evoked rat liver and kidney toxicity and to understand its molecular mechanism. 21 male Wistar albino rats were divided into 3 groups, as follows: group (1), normal saline; group (2), IM; and group (3), HFM. Both insecticides were orally administered every day for 28 days at a dose equal to 1/10 LD50 from the active ingredient. After 28 days postdosing, rats were anesthetized to collect blood samples then euthanized to collect liver and kidney tissue specimens. The results showed marked changes in walking, body tension, alertness, and head movement with a significant reduction in rats’ body weight in both IM and HFM receiving groups. Significant increases in MDA levels and decrease of GHS levels were recorded in liver and kidney homogenates of either IM or HFM groups. Liver and kidney tissues obtained from both pesticide receiving groups showed extensive histopathological alterations with a significant increase in the serum levels of ALT, AST, urea, and creatinine and a decrease in total proteins, albumin, and globulin levels. In addition, there was upregulation of the transcript levels of casp-3, JNK, and HO-1 genes with strong immunopositivity of casp-3, TNF-ὰ, and NF-KB protein expressions in the liver and kidneys of rats receiving either IM or HFM compared with the control group. In all studied parameters, HFM caused hepatorenal toxicity more than those induced by IM. We can conclude that each IM and HFM provoked liver and kidneys damage through overproduction of ROS, activation of NF-KB signaling pathways and mitochondrial/JNK-dependent apoptosis pathway.

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Effects of Four Local Species of Beans Consumed in South-eastern Nigeria on Selected Biochemical Indices of Wistar Albino Rats

10.54117/iijbs.v1i1.1 ◽

2021 ◽

Vol 1 (1) ◽

pp. 1-7

Author(s):

Jonathan C. Ifemeje ◽

◽

Chukwuebuka Egbuna ◽

Chukwuemelie Zedech Uche ◽

Michael Chinedu Olisah ◽

...

Keyword(s):

Phaseolus Vulgaris ◽

Albino Rats ◽

Composition Analysis ◽

Nutritional Factors ◽

Significant Difference ◽

Liver And Kidney ◽

Local Species ◽

Normal Rat ◽

Wistar Albino Rats ◽

Biochemical Indices

The chemical composition and the effects of four local species of beans on the liver and kidney function of Wistar albino rats were examined. The four beans species are Cajanus Cajan, Vigna unguiculata subsp. sesquipedalis, Phaseolus vulgaris L ‘Red kidney’, and Phaseolus vulgaris 'Black turtle’. The results obtained for the proximate composition analysis revealed that the protein content of the bean species was higher in P. vulgaris L ‘Red kidney’ (18.54±0.01a %), and P. vulgaris 'Black turtle’ (18.36±0.01a %) with no significant difference at P< 0.05. The phytochemical composition analysis revealed that the beans contain various phytochemicals including some anti-nutritional factors. The mineral composition analysis shows that V. u. subsp. Sesquipedalis had a higher level of minerals (4.80±4.43a mg/100g) followed by C. cajan (3.24±2.64b mg/100g). The experimental design comprises of 30 male Wistar albino rats distributed into 5 groups of 6 rats each. Each group except the control received 50g of normal rat feed + 100g of beans body weight. The effects of the beans species on the biochemical parameters suggest no adverse effects when compared to the control. Despite that, these beans contain high nutritional components that are of nutritional interest as as well as some anti-nutritional factors.

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