The nutrition of the early-weaned calf IV. Ruminal ammonia formation from soluble and insoluble protein sources

1963 ◽  
Vol 5 (2) ◽  
pp. 147-156 ◽  
Author(s):  
T. R. Preston ◽  
F. G. Whitelaw ◽  
N. A. MacLeod

SUMMARY1. Rumen-ammonia concentration, pH of rumen liquor and blood-urea concentration have been measured at intervals after feeding in six calves given diets containing ‘soluble’ groundnut meal, ‘soluble’ herring meal or ‘insoluble’ herring meal as the major protein source.2. There were no significant differences between diets in any of the measurements examined. A difference approaching significance was, however, observed between ‘soluble’ herring meal and the other diets in the direction and extent of the change in rumen-ammonia concentration during the first hour after feeding.3. On all diets, pH of rumen liquor fell to a value in the region of 5·5 immediately after feeding and increased gradually to around 6·5 during the following 8 hr. Rumen-ammonia concentrations were at all times very low.4. There was no significant residual correlation between changes in blood-urea concentration and the corresponding changes in rumen-ammonia concentration.5. Rumen-ammonia concentration increased markedly on the ‘soluble’ herring meal diet when the nitrogen intake of the animals was increased by 50%; under similar conditions, only slight increases in this measurement were observed on the other diets.6. Thesefindingsare discussed in relation to the efficiency of utilisation of dietary nitrogen.

2007 ◽  
Vol 74 (4) ◽  
pp. 412-416 ◽  
Author(s):  
Richard A Laven ◽  
D Claire Wathes ◽  
Kevin E Lawrence ◽  
Rex J Scaramuzzi

Measurement of plasma urea concentration is often used to identify a risk of dietary nitrogen-associated infertility. However, the use of plasma urea concentration in this way relies on it being an effective predictor for other potential toxic products associated with nitrogen metabolism (such as plasma or uterine ammonia). Recent research has shown that dietary nitrogen-associated infertility can be produced by diets which elevate plasma ammonia concentration without markedly increasing plasma urea concentration. Thus for cattle on different diets plasma urea concentration cannot be used to predict plasma ammonia concentration. This study evaluated whether plasma urea concentration could be used to predict plasma ammonia concentration in cattle kept on consistent diets. Data were analysed from a study where 42 cattle had been fed a control diet or the control diet plus 250 g urea per cow per day and had had weekly measurements of post-prandial plasma urea and ammonia concentrations. This analysis found that over a 100-d period, plasma urea concentration was relatively constant and unaffected by time while plasma ammonia concentration was significantly more variable, being affected by time since the study started, and whether cows began the study in the first or second group. Correlation between plasma ammonia and urea was limited; plasma urea concentration explained only 3·8% of the variation in plasma ammonia concentration. These data suggest that, even in cows on consistent diets, plasma urea concentration is not a good predictor of plasma ammonia, and that a simple urea threshold may not accurately identify the risk of dietary nitrogen-associated infertility.


1996 ◽  
Vol 34 (7-8) ◽  
pp. 79-85 ◽  
Author(s):  
Rengao Song ◽  
Roger Minear ◽  
Paul Westerhoff ◽  
Gary Amy

Empirical bromate formation models were developed from batch ozonation data to simulate the effects of important water quality characteristics and treatment processes on bromate formation. Bromate formation was favored at high pH, bromide concentration, alkalinity, and ozone dose. On the other hand, increasing DOC and ammonia concentration decreased bromate formation. Validation of the bromate models demonstrated that the models accurately simulated bromate formation. Risk analysis of bromate formation was performed on 5 utilities in which ozone was used, and it was concluded that under typical ozonation conditions, the associated risk related to bromate formation could be as high as 10−3.


2014 ◽  
Vol 881-883 ◽  
pp. 757-760
Author(s):  
Xiao Qing Ren ◽  
Li Zhen Ma ◽  
Xin Yi He

The objective of this study was to examine the effect of different levels of catfish bone paste to flour on the physicochemical, textural and crumb structure properties of steamed bread. Six different levels (0, 1, 3, 5, 7,10 %) of catfish bone paste to flour were used in the formulation of the steamed bread. The results showed that the weight loss and TTA of steamed bread decreased with an increase in the levels of the catfish bone paste. On the other hand, the pH increased with an increase in the levels of the catfish bone paste. The specific volume, hardness, chewiness and gas cell structure in the crumb of steamed bread with catfish bone paste at 5% supplementation level were better. Thus, a value of 5% catfish bone paste was considered a better level for incorporation into the steamed bread.


2014 ◽  
Vol 881-883 ◽  
pp. 766-775 ◽  
Author(s):  
Dan Wu ◽  
Wei Hong Min ◽  
Jing Sheng Liu ◽  
Li Fang ◽  
Hong Mei Li ◽  
...  

The functional properties of protein isolate and major protein fractions prepared from Changbai Mountain pine nuts were investigated. Albumin, globulin, glutelin, and protein isolates were obtained after the Osborne method and alkaline dissolution and acid precipitation, and protein contents of the fractions are 48.02%, 81.93%, 83.02%, and 89.69%, respectively. For the sulfhydryl contents, albumin is the highest, and glutelin is the lowest. In a disulphide bond, the protein isolate content is the highest with a value of 28.74 μmol/g, and the glutelin content is the lowest with the value of 13.46 μmol/g. For the four kinds of proteins, the essential amino acids in percentage of total amino acids are 31.13%, 34.22%, 30.30%, and 34.54%, respectively. The pH dependent protein solubility profile reveals that the minimum solubility is at pH 5.0, which corresponds to the isoelectric point. Protein isolate has the minimum water absorption capacity with a value of 0.59 ml/g. On the other hand, albumin has the minimum oil absorption capacity with a value of 2.11 ml/g. The emulsifying activity and stability and the foaming activity and stability increased with increasing concentration of four kinds of proteins. SDS-PAGE results showed that these four kinds of proteins have different molecules.


1970 ◽  
Vol 74 (2) ◽  
pp. 259-271 ◽  
Author(s):  
J. G. Morris ◽  
E. Payne

SUMMARYThe intravenous (i.v.) infusion of solutions of ammonium salts into sheep produced a toxic condition in which the clinical signs, pathological findings and concentrations of ammonia in the venous blood were comparable with those found in urea poisoning, provided that the urea and ammonia toxicoses were induced over similar time intervals. Our results indicate that urea toxicosis in ruminants is due to the toxic effects of ammonia. Although the clinical signs resulting from the i.v. infusion of ammonium chloride, acetate and hydroxide showed some relationship to the basicity of the compounds, alkalosis did not appear to be a necessary prerequisite for ammonia toxicosis.The tolerances of sheep to orally administered urea and i.v. infused ammonium salt solutionswere shown to be positively related to dietary nitrogen intake. These results and the observations reported by Payne & Morris (1969) that the concentrations of urea-cycle enzymes per unit of liver tissue were markedly affected by dietary nitrogen intake suggest that supplementation of ruminants grazing low-protein pastures with urea, occurs at a time when their tolerances to an over-dose of urea are minimal.The i.v. administration of arginine and of y-amino butyric acid plus glucose did not appear to be of practical value in preventing urea poisoning.


1954 ◽  
Vol 44 (3) ◽  
pp. 270-273 ◽  
Author(s):  
E. F. Annison ◽  
Margaret I. Chalmers ◽  
S. B. M. Marshall ◽  
R. L. M. Synge

1. Ruminal ammonia concentrations were measured in sheep receiving basal diets of hay and meals with various supplementary sources of protein. Ammonia production from groundnut meal was great and from maize products slight. Herring meal behaved in an intermediate way.2. The extent of ammonia production from a given amount of protein-rich material was decreased when the amount of starch or cereal meal fed at the same time was increased.


2006 ◽  
Vol 27 (2) ◽  
pp. 777-787 ◽  
Author(s):  
Julie A. Law ◽  
Sean O'Hearn ◽  
Barbara Sollner-Webb

ABSTRACT In trypanosome RNA editing, uridylate (U) residues are inserted and deleted at numerous sites within mitochondrial pre-mRNAs by an ∼20S protein complex that catalyzes cycles of cleavage, U addition/U removal, and ligation. We used RNA interference to deplete TbMP18 (band VII), the last unexamined major protein of our purified editing complex, showing it is essential. TbMP18 is critical for the U-deletional and U-insertional cleavages and for integrity of the ∼20S editing complex, whose other major components, TbMP99, TbMP81, TbMP63, TbMP52, TbMP48, TbMP42 (bands I through VI), and TbMP57, instead sediment as ∼10S associations. Additionally, TbMP18 augments editing substrate recognition by the TbMP57 terminal U transferase, possibly aiding the recognition component, TbMP81. The other editing activities and their coordination in precleaved editing remain active in the absence of TbMP18. These data are reminiscent of the data on editing subcomplexes reported by A. Schnaufer et al. (Mol. Cell 12:307-319, 2003) and suggest that these subcomplexes are held together in the ∼20S complex by TbMP18, as was proposed previously. Our data additionally imply that the proteins are less long-lived in these subcomplexes than they are when held in the complete editing complex. The editing endonucleolytic cleavages being lost when the editing complex becomes fragmented, as upon TbMP18 depletion, should be advantageous to the trypanosome, minimizing broken mRNAs.


1968 ◽  
Vol 25 (2) ◽  
pp. 393-407 ◽  
Author(s):  
John R. Hunter

Schools of six jack mackerel each were photographed with infrared film at eight levels of luminance and also in darkness. Three indices were used to measure the behavior of the school from motion pictures. Two of the indices, mean distance to nearest neighbor and mean separation distance, were measures of the distances between individuals in a school; the other, mean angular deviation, was a measure of differences in orientation between individuals. A value for each index was calculated for each motion picture frame.From 12.1 to 6 × 10−6 ft-L no differences existed in the angular deviation of the school or in the distances between fish. At 6 × 10−7 ft-L the intervals between fish were much larger than at higher levels of brightness and groups showed little uniformity in their orientation. Below 6 × 10−7 ft-L (darkness) schools were dispersed and the distributions of values of angular deviation were random.The ability of jack mackerel to feed on live adult Artemia was also tested at eight levels of luminance and in darkness. The number of Artemia eaten at 6 × 10−5 ft-L was about half of that eaten at the normal daytime level of 12.1 ft-L. Few Artemia were eaten at 6 × 10−7 ft-L and none in darkness.Comparison of these data with measurements of light in the sea indicated that jack mackerel probably would be able to maintain schools near the surface on a moonless starlit night and that they probably could feed effectively near the surface on a full moonlight night.


2015 ◽  
Vol 50 (2) ◽  
pp. 153-162 ◽  
Author(s):  
EI Adeyeye ◽  
AJ Adesina

The levels of fatty acids, phospholipids and sterols were determined in the brains of she-goat and castrated goat found in Ekiti State, Nigeria by gas chromatography. Results showed that the crude fat levels were 9.98 and 10.2 % in the brains of she-goat and castrated goat respectively. The fatty acid composition of she-goat and castrated goat brain shows that the SFA was 40.6 and 42.7 %, MUFA was 37.1 and 38.7 % and PUFA was 20.9 and 22.3% respectively. The other parameters of she-goat and castrated goat brain were found: PUFA/SFA, 0.490 and 0.548; MUFA/SFA, 0.869 and 0.953; n-6/n-3, 0.775 and 11.7; LA/ALA, 0.876 and 28.0; AA/DGLA, 6.05 and 17.4; EPA/DHA, 1.00 and 5.89 and EPSI (PUFA/MUFA), 0.564 and 0.575 respectively. Phospholipids were present in she-goat and castrated goat with a value range of 2365 and 3047 mg/100g respectively. Among the sterols, only cholesterol was of any significant level with values of 1353 mg/100g (she-goat brain) and 1355 mg/100g (castrated goat brain). Linear correlation at ? = 0.05, df: n-1 showed that no significant difference exists between the crude fats, phospholipids and sterols except in the fatty acids parameters.Bangladesh J. Sci. Ind. Res. 50(2), 153-162, 2015


Author(s):  
Adil Bakoğlu ◽  
Hüseyin Baykal ◽  
Muhammed İkbal Çatal

In this study, which was carried out in 2018, some vegetation characteristics of Handüzü plateau which was connected to Güneysu district of Rize province was determined by using Lup method. Canopy cover and botanical composition ratios and pasture status class were investigated in the study. As a result of the study, 4 grasses, 4 legume and 33 other family plants were identified. The rate canopy covering of the research area was 82.40%, the rate of grasses in the botanical composition was 33.37%, the rate of the legumes was 5.75% and the ratio of the other families was 60.88%. The first three species, the most common found in pasture, respectively; Nardus stricta L. (27.00%), Carex atrata L. subsp. aterrima (Hoppe) Hartm. (11.10%) and Polygala alpestris Rchb. (8.50%). Pasture status was determined as weak with a value of 2.456% degree of pasture quality. It was concluded that especially controlled grazing and top-seeding, were applied together other methods of breeding.


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