scholarly journals Seroprevalence of IgG against conformational and linear capsid antigens of parvovirus B19 in Italian blood donors

2004 ◽  
Vol 132 (5) ◽  
pp. 857-862 ◽  
Author(s):  
E. MANARESI ◽  
G. GALLINELLA ◽  
A. M. MORSELLI LABATE ◽  
P. ZUCCHELLI ◽  
D. ZACCARELLI ◽  
...  

Serum samples from 446 Italian blood donors between 18 and 65 years of age were analysed for the presence of IgG against parvovirus B19 capsid proteins VP1 and VP2 including conformational and linear epitopes. The overall prevalence of IgG against parvovirus B19 capsid proteins VP1 and VP2 against at least one antigen type was 79·1%. No significant difference was found between men and women. In the 18–27 years age group, 77·0% of the population had experienced infection with the virus, reaching 88·5% in the 48–57 years age group. The overall prevalence of IgG was 78·0% against conformational VP1+VP2 antigens, 74·9% against conformational VP2, 70·9% against linear VP1 and 23·3% against linear VP2 in the analysis of the IgG response against different conformational and linear epitopes of VP1 and VP2. Although IgG against conformational VP1+VP2, conformational VP2 and linear VP1 was present in more than 60% of subjects in all age groups, IgG against VP2 linear antigens was present in only 32% of subjects in the 18–27 years age group and then decreased to 20·5% in the 28–37 years age group. A different trend was noted when IgG positivity against linear and conformational epitopes was analysed separately in men and women. A significant increase was found in seroprevalence of IgG against VP2 conformational antigens with increasing age in males and a significant decrease in seroprevalence of IgG against VP2 linear antigens in women with increasing age.

2001 ◽  
Vol 8 (1) ◽  
pp. 161-165 ◽  
Author(s):  
Mitsutaka Kuzuya ◽  
Ritsushi Fujii ◽  
Masako Hamano ◽  
Ritsuko Ohata ◽  
Hajime Ogura ◽  
...  

ABSTRACT A novel blocking enzyme-linked immunosorbent assay (BL-ELISA) was developed for detection of antibodies to human group C rotavirus (CHRV). The specificity of the BL-ELISA was confirmed by using animal sera hyperimmunized to group A and group C rotaviruses and paired sera from five patients with acute CHRV gastroenteritis. Furthermore, there was concordance between the BL-ELISA and a neutralization assay for CHRV in 226 (95%) of 238 samples. By using the BL-ELISA, we determined the seroprevalence of CHRV in 704 serum samples obtained from nine different age groups of inhabitants of Okayama Prefecture, Japan, in 1992, 1994, and 1996. As a result, 211 sera (30%) were found to be positive for CHRV antibodies. The seroprevalence gradually increased with age and reached 52.7% in the oldest individuals. A further analysis of the youngest age group suggested that CHRVs predominantly prevail in persons older than 3 years of age in Japan. When comparing the three sampling years, a larger percentage of antibody-positive sera was detected in 1994 than in either 1992 or 1996 in individuals between 6 and 15 years of age, reflecting the occurrence of a CHRV outbreak among children during the winter of 1992 to 1993 that was previously documented. These results indicate that CHRV infections may occur more frequently in spite of the relatively low detection rate of the virus.


2017 ◽  
Vol 20 (5) ◽  
pp. 329-334 ◽  
Author(s):  
Svetlana V. Mustafina ◽  
Oksana D. Rymar ◽  
Sofiya K. Malyutina ◽  
Diana V. Denisova ◽  
Liliya V. Shcherbakova ◽  
...  

Aims. To estimate the prevalence of type 2 diabetes mellitus (T2DM) in different age groups of the adult population of Novosibirsk, according to epidemiological studies in 20032005 and 20132016. Methods. We examined a representative population sample (assessed in 20032005) of men and women aged 4569 years in two administrative districts of Novosibirsk, as a part of the international HAPIEE project. According to the tables of random numbers, representative samples of men and women aged 4569 years were formed, to which letters were sent, inviting them to pass for examination. During 20132016, a second population survey was conducted on a random representative sample of a population of 2544-year-olds of both sexes. Participants were residents of one of the districts of Novosibirsk. T2DM and impaired fasting glucose (IFG) were diagnosed using fasting plasma glucose (FPG) levels (diabetes: FPG 7.0 mmol/l; IFG: FPG 6.16.9 mmol/l). Results. The prevalence of T2DM among residents aged 4569 years was 11.3%, and overall, no significant difference in prevalence was found between females and males (11.3% vs. 11.0%). However, the overall prevalence of T2DM among residents aged 2544 years was 2.2%, and prevalence was higher in men (3.5%) than in women (1.1%), p 0.05. High prevalence of IFG was found in the 4569 age group (18.2%, in 20032005), and in the younger age group of 2544 years (21.6%, in 20132016). The high rate among young individuals is particularly alarming. Less than one half (4.8 out of 11.3%) of participants aged 4569 tested positive for T2DM, and only one in the age group 2544 years knew he/she had diabetes. This indicates a lack of knowledge among Siberians about their problem with diabetes. Conclusion. Approximately one in five adults had IFG. Among the adult population aged 4569 (in 20032005), 18.2% had IFG and 11.3% had T2DM. In individuals aged 2544 years in 20132016, IFG was observed in 21.6%, and 2.2% had T2DM (p0.05).


Author(s):  
Dhruv Desai ◽  
Pushpa Makwana ◽  
Jayesh Solanki ◽  
Irshadullakhan Kalyani ◽  
Dharmesh Patel ◽  
...  

Aims: Leptospirosis is endemically prevalent in coastal area of south Gujarat. Generally, pets are vaccinated against Leptospira interrogans but whosoever left unvaccinated, are prone to infection. The present study was conducted to detect canine leptospirosis and its prevalence from south Gujarat. Place and Duration of Study: A total of 46 serum samples and 33 urine samples were collected from 56 dogs suspected of having leptospira infection. The study was done from September 2019 to May 2020. Methodology: Serum samples were diagnosed by IgG based ImmunoComb solid phase dot ELISA test for antibody against genus specific Leptospira interrogans. Urine samples were visualized under dark field microscopy (DFM) following laboratory manual on leptospirosis of WHO. Sex and age of dogs were noted to find out the percent positivity and prevalence. Chi square test was applied to find out any significant difference among the age groups. Results: Out of 46 serum samples, 17 (36.95%) found positive by dot ELISA and out of 33 urine sample, 13 (39.39%) found positive by DFM. Sex wise prevalence found 42.10 percent (16/38) in Male while 55.55 percent (10/18) in female dogs. Age group wise prevalence was recorded 63.63 percent (07/11) in 1- ≤ 3 years group. 27.77 per cent (05/18) in > 3- ≤ 6 years group whereas, 51.85 percent (14/27) in > 6- ≤ 12 years group. Conclusion: Detection and prevalence of canine leptospirosis were studied first time from Gujarat. Overall 46.42 percent (26/56) dogs were diagnosed positive for canine leptospirosis. Dogs having age group of 1- ≤ 3 years found more susceptible. Prevalence was found more in female than male dogs. 


2020 ◽  
Vol 30 (4) ◽  
pp. 453-462
Author(s):  
O. O. Salagay ◽  
G. M. Sakharova ◽  
N. S. Antonov

In the Russian Federation, after the adoption of Federal Law “On the Protection of Citizens' Health from the Effects of Tobacco Smoke and the Consequences of Tobacco Use”, the prevalence of smoking tobacco has significantly decreased. Reducing the consumption of smoking tobacco products led to the emergence of a new aggressive policy of tobacco companies, which resulted in the emergence of new “innovative” products and the concepts of “less harmful tobacco product” or “less harmful nicotine-containing product”. The undoubtedly aggressive advertising campaigns of tobacco companies and manufacturers of electronic cigarettes have affected the smoking behavior of tobacco consumers, which has begun to change in recent years.The purpose of this study was to study new trends in the structure of consumption of tobacco and nicotine-containing products in various age groups of the Russian Federation, as well as among men and women.Methods. Analysis of the structure of consumption of tobacco and nicotine-containing products was carried out as part of an online public opinion poll to study the level of awareness of measures to reduce the prevalence of tobacco products in the Russian Federation, initiated in 2019 by the Ministry of Health of the Russian Federation. A total of 1 282 respondents from 73 regions of the Russian Federation took part in the survey. Among the respondents there were 447 (34.9%) men and 835 women (65.1%) aged 18 years and younger to 70 years. To conduct a comparative analysis of the structure of use of tobacco and nicotine-containing products, the frequencies of their use in different age groups, among men and women, were calculated. A statistically significant difference between the groups was confirmed by analyzing the dependence in the contingency table using the χ2 criterion.Results. A total of 432 respondents reported the use of tobacco and nicotine-containing products, which amounted to 33.7% of all respondents. The most used products were smoking tobacco (57.64%), electronic cigarettes (34.03%) and waterpipe (33.80%). However, in different age groups, the structure of their use was significantly different (p < 0.05). The highest level of electronic cigarette use, including vapors containing nicotine and electronic nicotine delivery product, were found in the age group of 18 years and younger (58.82%). In the older age groups, a significant decrease in the level of consumption was observed. Chewing tobacco and snuff were consumed by consumers significantly less than other tobacco products. Smokeless tobacco was mainly consumed by consumers aged 18 years and younger, its level was 20.58%. The highest level of waterpipe consumption was found among consumers in the age group of 19 – 29 years (53.85%), it was slightly less in the group of 18 years and younger (44.11%). The higher level of waterpipe consumption was detected (40.35%) among women than it was among men (26.47%). Many consumers indicated that they consumed several types of tobacco and nicotine-containing products. The most popular combination of products was found among consumers under the age of 30, and 20.59% of consumers aged 18 and younger consumed at the same time almost all the types of tobacco and nicotine-containing products that were presented on the market.Conclusion. The study revealed new trends in preferences for consumption of tobacco and nicotine-containing products. The new trends have significantly changed the structure of the consumption of these products among the population of Russian Federation.


2008 ◽  
Vol 2 ◽  
pp. 24-27
Author(s):  
Md Shibbir Ahmed ◽  
Mohsin Khalil ◽  
Md Babibur Rahman ◽  
Sabina Mannan ◽  
Seheli Zannat Sultana ◽  
...  

The morphological study was done to see length, breadth and thickness of the ovary in different age group of Bangladeshi people to increase the knowledge regarding variational anatomy in our population. Sixty two postmortem tissue block containing ovary and fallopian tube along with surrounding structures were collected from 62 female cadavers of different age groups and fixed in 10% formol saline solution. Gross & fine dissections were carried out to study these morphological parameters of ovary in different age group. In the present study, findings were compared with the finding of other researchers.In this study the mean length of ovary was maximum in found in group C (46-80 years) 4.32cm and minimum was found in group A (2-13 years) 2.81cm of both side. The mean breadth of ovary was maximum in group C 2.01cm and minimum was in group A 1.38cm. The mean thickness of ovary maximum was in group C 0.971cm and minimum was in group A 0.682cm of both sides. In statistical analyses significant difference between two groups was calculated by using students ‘t' test. A difference between two groups was considered to be significant when p


Author(s):  
Jerold C. Alcantara ◽  
Farhan Khalaf M. Alenezi ◽  
Omar Hafiz Haj Ali

Background: Evaluating the trends and rates of transfusion-transmitted infections (TTIs) among blood donors ensure that supplies of blood are safe and a proficient donor screening is in place. Hence, the study assessed the prevalence and trends of TTI markers among blood donors in Hail, Saudi Arabia.Methods: A retrospective review was done using donor records in a 3-year period from January 2013 to December 2015. All samples underwent to mandatory serological screening. Descriptive and inferential statistics were used appropriately. Distribution tables were developed to discern the trends that exist and compare the prevalence rates among different age groups. Significant level was set at 5% (p<0.05).Results: Of the 11,162 blood donors evaluated, 1.5% was found positive for TTI markers. The overall prevalence of HBV, HCV, HTLV I/II, syphilis and malaria were 1.2, 0.04, 0.07, 0.2 and 0.02%, respectively. No blood donor showed positive for HIV markers. Majority of the infections were evident among replacement donors (57.3%). Greater number was observed from the age group of 30-39 years. Statistically, there was no significant difference among the various age groups.Conclusions: The prevalence rate of TTIs in this study is comparably lower than in other regions of Saudi Arabia and some countries, with no significant variation among the different age groups. HBV continues to be the highest rate of infections inflicting blood donors. Methods to improve donor retention, encouragement and recruitment of new donors have to be identified. 


2021 ◽  
pp. 112067212110233
Author(s):  
Marcelina Sobczak ◽  
Magdalena Asejczyk ◽  
Malwina Geniusz

Objectives: The main goal of this research was to determine the differences between the values of intraocular pressure (IOP) in the supine and sitting positions, and to assess the effect of age and cardiovascular parameters. Methods: Seventy-two healthy adults were enrolled and classified into age groups: 20–30 years (group A), 31–40 years (group B), and 41–71 years (group C). Corneal biometry and cardiovascular parameters, such as heart rate (HR), were measured. IOP measurements were taken in the sitting position (IOPS) and in the supine position (IOPL) using the iCare® Pro tonometer. Results: A significant difference between the IOPS and IOPL in the entire cohort was found ( p < 0.001). Regarding the age subgroups, a significant difference ( p < 0.001) between the IOPS and IOPL was obtained in group A (2.6 ± 1.6 mmHg) and group C (1.5 ± 1.3 mmHg). There were no significant differences in the IOPS between groups. The highest IOP values were obtained for group A. The correlations between HR and IOPS are statistically significant for group A and group B, and for HR and IOPL-S for group B only. Multivariate analysis showed that HR has a significant influence on the difference in IOP in the two body positions. Conclusion: A statistically significant difference between the effect of age and the values of IOPS and IOPL was shown. Cardiovascular parameters showed some relevant statistical dependencies, but with a rather marginal significance in young people. The influence of body position for the measurement of IOP for healthy subjects does not seem to matter, despite the fact that there are some dependencies that are statistically significant.


2011 ◽  
Vol 26 (S2) ◽  
pp. 1887-1887
Author(s):  
T. Butkova ◽  
N. Kibrik

ObjectiveFamily-sexual disharmonies - are the frequent reasons of suicide behaviour. Design: Complex inspection of 62 patients, in the age from 18 years has been performed. In the anamnesis patients had suicide behaviour owing to family-sexual disharmonies.Results1.Age when suicide attempts have been accomplished. Middle age has made 31 ± 8,8 years; 20–29 years - 43% (27 person); 30-39 years - 39% (24 person). The other 11 person-were included into the age groups of 40–49, 50–59 years.2.The reasons of autoaggressive behaviour: Suicide attempts owing to divorce, ideas of matrimonial incorrectness - 52% (32 person).Owing to the unfair attitude from the nearest environment or a dissatisfaction with behaviour and personal qualities of significant another - 48% (30 person). Age features: For young age - unavailability of young spouses to home life, absence of tolerance and respect to each other. Average age group - a dissatisfaction with behaviour of the partner. The senior age group - somatic diseases, loneliness, loss of relatives 3. In 66% of cases (41 person) suicide had no in the anamnesis of mental diseases at relatives.ConclusionsThe primary goal at rendering the psychotherapeutic help - revealing and correction of non adaptive installations of the person. Formation of confidential and empathic attitudes in family. It has been confirmed correlation analysis between partners′ satisfaction attitudes, personal characteristics suicident. It is necessary to raise availability of the information on various aspects of sexual health, psychohygiene of intimate relations.


2011 ◽  
Vol 35 (1) ◽  
pp. 16-24
Author(s):  
Lazem H. Al-Taie

Back ground: Toxoplasmosis is an important zoonosis that causes economic losses in animal herds due to abortion and stillbirth as well as changes in the reproductive and neural system of susceptible animals . Objective: The aims of the present study is to determination the prevalence of T. gondii in farm animals ( sheep& goat)of both genders and different ages in Sulaimani province by using two serological tests (ELISA and LAT). Methods: Blood samples were collected from farm animals ,142 sheep and 46 goats , of different sexes and ages. Tow different serological tests ,ELISA and LAT for qualitative determination of T. gondii antibody titer in sheep and goats serum samples. Results: The prevalence rate in sheep was 73 (51.7 %) and 82 (57 %) , and 21 (54.6 %) and 25 (54.35 %) in goats ,by ELISA and LAT respectively. The prevalence of toxoplasmosis was highest in age group 7-9 (66.6%) in sheep in compares’ with other age groups. There was no significant differences between both spp.and tow test. Conclusion: Statistical results show no significant differences between both tests (ELISA &LAT) at (P ≥ 0.05).The prevalence of toxoplasmosis was increased proportionally with the age of animals, while gender has no effect on the prevalent rate .


2020 ◽  
Vol 6 (1) ◽  
pp. 6-10
Author(s):  
Chhabi Lal Adhikari ◽  
Guru Prasad Dhakal ◽  
Nongluck Suwisith ◽  
Sonam Dargay ◽  
Krishna P Sharma

Introduction: Helicobacter pylori (H. pylori) is a bacterium causing chronic gastric infection and may cause gastric cancer. It was necessary to see the trend of infection, especially in symptomatic patients. This retrospective descriptive study was aimed to describe the characteristics of H. pylori infection in Bhutanese patients referred for an endoscopy to the National Referral Hospital, Thimphu. Methods: The sample of the study was randomized 380 medical records of the patients who underwent upper gastrointestinal endoscopy and Rapid Urea Test for symptomatic dyspepsia and peptic ulcer. Data was collected using a survey form designed by the researchers. Data analysis was done using descriptive statistics and either Chi-square or Fisher’s exact test. Results: The prevalence of H. pylori infection was very high (76.6%). The mean age of the infection was 42 with a range from 15 to 84 years. The highest prevalence of infection was observed in the age group 20-29 years (82.7%) and lowest in the oldest age group 70-84 years (66.7%). The analysis showed no significant difference in infection amongst age groups, gender, and endoscopic findings to the positive results at 5% significant level except for monthly prevalence (p<0.001). Gastritis was the commonest endoscopy finding (153/380) and gastro-duodenitis had the highest positivity rate (88.9%). Conclusion: The prevalence of infection was relatively high compared with previous studies. Young and middle-aged adults had a high prevalence and this group needs to be given priority for screening and eradication treatment considering limited resources to prevent associated gastric cancer in Bhutan.


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