Decellularized Extracellular Matrix Composite Hydrogel Bioinks for the Development of 3D Bioprinted Head and Neck in Vitro Tumor Models

2021 ◽  
Author(s):  
Jacqueline Kort-Mascort ◽  
Guangyu Bao ◽  
Osama Elkashty ◽  
Salvador Flores-Torres ◽  
Jose G. Munguia-Lopez ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Head And Neck ◽  
Matrix Composite ◽  
Composite Hydrogel ◽  
Tumor Models ◽  
Decellularized Extracellular Matrix

Investigation of an injectable gold nanoparticle extracellular matrix

2021 ◽  
pp. 088532822110515
Author(s):  
Colten Snider ◽  
David Grant ◽  
Sheila A Grant
Keyword(s):  
Extracellular Matrix ◽  
Synovial Fibroblasts ◽  
X Ray ◽  
Decellularized Extracellular Matrix ◽  
Intracellular Ros ◽  
Murine Fibroblasts ◽  
Post Traumatic ◽  
Injection Force ◽  
20 Nm

Post-traumatic osteoarthritis (PTOA) is a progressive articular degenerative disease that degrades articular cartilage and stimulates apoptosis in chondrocyte cells. An injectable decellularized, extracellular matrix (ECM) scaffold, that might be able to combat the effects of PTOA, was developed where the ECM was conjugated with 20 nm gold nanoparticles (AuNP) and supplemented with curcumin and hyaluronic acid (HA). Porcine diaphragm ECM was decellularized and homogenized; AuNPs were conjugated using chemical crosslinking followed by mixing with curcumin and/or HA. Injection force testing and scanning electron microscopy with energy-dispersive X-ray spectroscopy were utilized to characterize the ECM scaffolds. In vitro testing with L929 murine fibroblasts, equine synovial fibroblasts, and Human Chondrocytes were used to determine biocompatibility, reactive oxygen species (ROS) reduction, and chondroprotective ability. The results demonstrated that conjugation of 20 nm AuNPs to the ECM was successful without significantly altering the physical properties as noted in the low injection force. In vitro work provided evidence of biocompatibility with a propensity to reduce intracellular ROS and an ability to mitigate apoptosis of chondrocyte cells stimulated with IL-1β, a known apoptosis inducing cytokine. It was concluded that an injectable AuNP-ECM may have the ability to mitigate inflammation and apoptosis.

scholarly journals Injectable Alginate-Peptide Composite Hydrogel as a Scaffold for Bone Tissue Regeneration

Nanomaterials ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 497 ◽  
Author(s):  
Moumita Ghosh ◽  
Michal Halperin-Sternfeld ◽  
Itzhak Grinberg ◽  
Lihi Adler-Abramovich
Keyword(s):  
Extracellular Matrix ◽  
Bone Regeneration ◽  
Composite Scaffold ◽  
Three Dimensional ◽  
Composite Hydrogel ◽  
Bone Tissue Regeneration ◽  
Pcr Analysis ◽  
Alizarin Red ◽  
In Vitro Biocompatibility

The high demand for tissue engineering scaffolds capable of inducing bone regeneration using minimally invasive techniques prompts the need for the development of new biomaterials. Herein, we investigate the ability of Alginate incorporated with the fluorenylmethoxycarbonyl-diphenylalanine (FmocFF) peptide composite hydrogel to serve as a potential biomaterial for bone regeneration. We demonstrate that the incorporation of the self-assembling peptide, FmocFF, in sodium alginate leads to the production of a rigid, yet injectable, hydrogel without the addition of cross-linking agents. Scanning electron microscopy reveals a nanofibrous structure which mimics the natural bone extracellular matrix. The formed composite hydrogel exhibits thixotropic behavior and a high storage modulus of approximately 10 kPA, as observed in rheological measurements. The in vitro biocompatibility tests carried out with MC3T3-E1 preosteoblast cells demonstrate good cell viability and adhesion to the hydrogel fibers. This composite scaffold can induce osteogenic differentiation and facilitate calcium mineralization, as shown by Alizarin red staining, alkaline phosphatase activity and RT-PCR analysis. The high biocompatibility, excellent mechanical properties and similarity to the native extracellular matrix suggest the utilization of this hydrogel as a temporary three-dimensional cellular microenvironment promoting bone regeneration.

scholarly journals The effect of cartilage decellularized extracellular matrix-chitosan compound on treating knee osteoarthritis in rats

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e12188
Author(s):  
Deng Chen ◽  
Yaxin Zhang ◽  
Qun Lin ◽  
Duoyun Chen ◽  
Xiaolei Li ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Knee Osteoarthritis ◽  
Therapeutic Effect ◽  
Animal Experiments ◽  
Common Disease ◽  
Alamar Blue ◽  
Decellularized Extracellular Matrix ◽  
Chondrocyte Death ◽  
Bone Injury

Knee osteoarthritis (KOA) refers to a common disease in orthopaedics, whereas effective treatments have been rarely developed. As indicated from existing studies, chondrocyte death, extracellular matrix degradation and subchondral bone injury are recognized as the pathological basis of KOA. The present study aimed to determine the therapeutic effect of decellularized extracellular matrix-chitosan (dECM-CS) compound on KOA. In this study, rat knee cartilage was decellularized, and a satisfactory decellularized extracellular matrix was developed. As suggested from the in vitro experiments, the rat chondrocytes co-cultured with allogeneic dECM grew effectively. According to the results of the alamar blue detection, dECM did not adversely affect the viability of rat chondrocytes, and dECM could up-regulate the genes related to the cartilage synthesis and metabolism. As reported from the animal experiments, dECM-CS compound could protect cartilage, alleviate knee joint pain in rats, significantly delay the progress of KOA in rats, and achieve high drug safety. In brief, dECM-CS compound shows a good therapeutic effect on KOA.

scholarly journals Cell-derived and enzyme-based decellularized extracellular matrix exhibit compositional and structural differences that are relevant for its use as a biomaterial

2021 ◽  
Author(s):  
Svenja Nellinger ◽  
Ivana Mrsic ◽  
Silke Keller ◽  
Simon Heine ◽  
Alexander Southan ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Adipose Tissue ◽  
Cultured Cells ◽  
Structural Characteristics ◽  
Human Adipose Tissue ◽  
Minimal Invasive ◽  
Healthcare Applications ◽  
Decellularized Extracellular Matrix

Due to its availability and minimal invasive harvesting human adipose tissue-derived extracellular matrix (dECM) is often used as a biomaterial in various tissue engineering and healthcare applications. Next to dECM, cell-derived ECM (cdECM) can be generated by and isolated from in vitro cultured cells. So far both types of ECM were investigated extensively towards their application as (bio)material in tissue engineering and healthcare. However, a systematic characterization and comparison of soft tissue dECM and cdECM is still missing. In this study, we characterized dECM from human adipose tissue, as well as cdECM from human adipose-derived stem cells (ASCs), towards their molecular composition, structural characteristics, and biological purity. The dECM was found to exhibit higher levels of collagens and lower levels of sulfated glycosaminoglycans (sGAGs) compared to cdECMs. Structural characteristics revealed an immature state of the fibrous part of cdECM samples. By the identified differences, we aim to support researchers in the selection of a suitable ECM-based biomaterial for their specific application and the interpretation of obtained results.

scholarly journals Further structural characterization of ovine forestomach matrix and multi-layered extracellular matrix composites for soft tissue repair

2021 ◽  
pp. 088532822110457
Author(s):  
Matthew J Smith ◽  
Sandi G Dempsey ◽  
Robert W Veale ◽  
Claudia G Duston-Fursman ◽  
Chloe A F Rayner ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Hyaluronic Acid ◽  
Soft Tissue ◽  
Tissue Repair ◽  
Matrix Composites ◽  
Soft Tissue Repair ◽  
Decellularized Extracellular Matrix ◽  
Biological Functionality ◽  
Traditional Approaches

Decellularized extracellular matrix (dECM)–based biomaterials are of great clinical utility in soft tissue repair applications due to their regenerative properties. Multi-layered dECM devices have been developed for clinical indications where additional thickness and biomechanical performance are required. However, traditional approaches to the fabrication of multi-layered dECM devices introduce additional laminating materials or chemical modifications of the dECM that may impair the biological functionality of the material. Using an established dECM biomaterial, ovine forestomach matrix, a novel method for the fabrication of multi-layered dECM constructs has been developed, where layers are bonded via a physical interlocking process without the need for additional bonding materials or detrimental chemical modification of the dECM. The versatility of the interlocking process has been demonstrated by incorporating a layer of hyaluronic acid to create a composite material with additional biological functionality. Interlocked composite devices including hyaluronic acid showed improved in vitro bioactivity and moisture retention properties.

scholarly journals Decellularized Extracellular Matrix for Cancer Research

Materials ◽  
2019 ◽  
Vol 12 (8) ◽  
pp. 1311 ◽  
Author(s):  
Takashi Hoshiba
Keyword(s):  
Cancer Research ◽  
Extracellular Matrix ◽  
Cancer Cell ◽  
Cancer Progression ◽  
Intracellular Signaling ◽  
Genetic Mutation ◽  
Cell Behavior ◽  
Characterization Methods ◽  
Decellularized Extracellular Matrix

Genetic mutation and alterations of intracellular signaling have been focused on to understand the mechanisms of oncogenesis and cancer progression. Currently, it is pointed out to consider cancer as tissues. The extracellular microenvironment, including the extracellular matrix (ECM), is important for the regulation of cancer cell behavior. To comprehensively investigate ECM roles in the regulation of cancer cell behavior, decellularized ECM (dECM) is now used as an in vitro ECM model. In this review, I classify dECM with respect to its sources and summarize the preparation and characterization methods for dECM. Additionally, the examples of cancer research using the dECM were introduced. Finally, future perspectives of cancer studies with dECM are described in the conclusions.

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