Two-Stage Desorption-Controlled Release of Fluorescent Dye and Vitamin from Solution-Blown and Electrospun Nanofiber Mats Containing Porogens

2013 ◽  
Vol 10 (12) ◽  
pp. 4509-4526 ◽  
Author(s):  
S. Khansari ◽  
S. Duzyer ◽  
S. Sinha-Ray ◽  
A. Hockenberger ◽  
A. L. Yarin ◽  
...  
Keyword(s):  
Controlled Release ◽  
Fluorescent Dye ◽  
Electrospun Nanofiber ◽  
Two Stage ◽  
Nanofiber Mats

scholarly journals Electrospun Nanofibrous Membranes for Tissue Engineering and Cell Growth

2021 ◽  
Vol 11 (15) ◽  
pp. 6929
Author(s):  
Ewin Tanzli ◽  
Andrea Ehrmann
Keyword(s):  
Tissue Engineering ◽  
Cell Growth ◽  
Polymer Blends ◽  
Mammalian Cells ◽  
Biological Properties ◽  
Specific Substrate ◽  
Electrospun Nanofiber ◽  
Materials Used ◽  
Nanofiber Mats ◽  
Surface Morphologies

In biotechnology, the field of cell cultivation is highly relevant. Cultivated cells can be used, for example, for the development of biopharmaceuticals and in tissue engineering. Commonly, mammalian cells are grown in bioreactors, T-flasks, well plates, etc., without a specific substrate. Nanofibrous mats, however, have been reported to promote cell growth, adhesion, and proliferation. Here, we give an overview of the different attempts at cultivating mammalian cells on electrospun nanofiber mats for biotechnological and biomedical purposes. Starting with a brief overview of the different electrospinning methods, resulting in random or defined fiber orientations in the nanofiber mats, we describe the typical materials used in cell growth applications in biotechnology and tissue engineering. The influence of using different surface morphologies and polymers or polymer blends on the possible application of such nanofiber mats for tissue engineering and other biotechnological applications is discussed. Polymer blends, in particular, can often be used to reach the required combination of mechanical and biological properties, making such nanofiber mats highly suitable for tissue engineering and other biotechnological or biomedical cell growth applications.

scholarly journals Real‐Time In Vivo Detection of Cellular Senescence through the Controlled Release of the NIR Fluorescent Dye Nile Blue

2020 ◽  
Vol 59 (35) ◽  
pp. 15152-15156 ◽  
Author(s):  
Beatriz Lozano‐Torres ◽  
Juan F. Blandez ◽  
Irene Galiana ◽  
Alba García‐Fernández ◽  
María Alfonso ◽  
...  
Keyword(s):  
Controlled Release ◽  
Real Time ◽  
Cellular Senescence ◽  
Nile Blue ◽  
Fluorescent Dye ◽  
In Vivo Detection

Ciprofloxacin-loaded alginate/poly (vinyl alcohol)/gelatin electrospun nanofiber mats as antibacterial wound dressings

2021 ◽  
pp. 152808372199746
Author(s):  
Tittaya Thairin ◽  
Patcharaporn Wutticharoenmongkol
Keyword(s):  
Weight Loss ◽  
Vinyl Alcohol ◽  
Diffusion Method ◽  
Poly Vinyl Alcohol ◽  
Burst Release ◽  
Electrospun Nanofiber ◽  
Total Immersion ◽  
Water Swelling ◽  
Nanofiber Mats ◽  
And Diffusion

Herein, ciprofloxacin (CIP)-loaded alginate/poly (vinyl alcohol)/gelatin (SPG) (CIP–SPG) nanofiber mats were successfully fabricated by electrospinning. The average fiber diameters of the mats before and after crosslinking were in the range of 190–260 and 385–484 nm, respectively. The chemical integrity of CIP remained intact after encapsulation into the mats. The degree of weight loss and water swelling decreased with an increase in the gelatin content of the electrospun nanofiber mats. A release study was carried out by total immersion and diffusion methods using phosphate buffer as a release medium. Burst release of CIP was observed in case of the total immersion method, while a more sustained release was observed in case of the diffusion method. The maximum amounts of CIP released during total immersion and diffusion were in the range of 70–90% and 72–85%, respectively. For both the total immersion and diffusion methods, the released amounts of CIP decreased and the release slowed down with an increase in the gelatin content; this result is consistent with the weight loss and water swelling values. The Young’s modulus increased, while the tensile strength and strain at break decreased with an increase in the gelatin content. The CIP–SPG nanofiber mats were slightly toxic to L929 mouse fibroblasts as evaluated by indirect cytotoxicity assay. The electrospun CIP–SPG nanofiber mats exhibited excellent antimicrobial activity against Staphylococcus aureus and Escherichia coli. These results reveal that the electrospun CIP–SPG nanofiber mats are potentially promising materials for wound healing applications.

scholarly journals A general strategy for generating gradients of bioactive proteins on electrospun nanofiber mats by masking with bovine serum albumin

2017 ◽  
Vol 5 (28) ◽  
pp. 5580-5587 ◽  
Author(s):  
Michael L. Tanes ◽  
Jiajia Xue ◽  
Younan Xia
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Electrospun Nanofibers ◽  
General Strategy ◽  
Electrospun Nanofiber ◽  
Bioactive Proteins ◽  
Nanofiber Mats

Gradients of bioactive proteins on mats of electrospun nanofibers were generated by masking with bovine serum albumin.

Lithographically patterned stretchable metallic microwiring on electrospun nanofiber mats

2021 ◽  
Vol 39 (6) ◽  
pp. 062801
Author(s):  
Yutika Badhe ◽  
Pedro E. Rocha-Flores ◽  
Walter E. Voit ◽  
David Remer ◽  
Lauren Costella ◽  
...  
Keyword(s):  
Electrospun Nanofiber ◽  
Nanofiber Mats

scholarly journals Electrospinning on 3D Printed Polymers for Mechanically Stabilized Filter Composites

Polymers ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 2034 ◽  
Author(s):  
Tomasz Kozior ◽  
Al Mamun ◽  
Marah Trabelsi ◽  
Martin Wortmann ◽  
Sabantina Lilia ◽  
...  
Keyword(s):  
3D Printing ◽  
Thermoplastic Polyurethane ◽  
High Surface ◽  
Antimicrobial Properties ◽  
Mechanical Damage ◽  
Volume Ratio ◽  
Electrospun Nanofiber ◽  
Polymer Nanofiber ◽  
3D Printed ◽  
Nanofiber Mats

Electrospinning is a frequently used method to prepare air and water filters. Electrospun nanofiber mats can have very small pores, allowing for filtering of even the smallest particles or molecules. In addition, their high surface-to-volume ratio allows for the integration of materials which may additionally treat the filtered material through photo-degradation, possess antimicrobial properties, etc., thus enhancing their applicability. However, the fine nanofiber mats are prone to mechanical damage. Possible solutions include reinforcement by embedding them in composites or gluing them onto layers that are more mechanically stable. In a previous study, we showed that it is generally possible to stabilize electrospun nanofiber mats by 3D printing rigid polymer layers onto them. Since this procedure is not technically easy and needs some experience to avoid delamination as well as damaging the nanofiber mat by the hot nozzle, here we report on the reversed technique (i.e., first 3D printing a rigid scaffold and subsequently electrospinning the nanofiber mat on top of it). We show that, although the adhesion between both materials is insufficient in the case of a common rigid printing polymer, nanofiber mats show strong adhesion to 3D printed scaffolds from thermoplastic polyurethane (TPU). This paves the way to a second approach of combining 3D printing and electrospinning in order to prepare mechanically stable filters with a nanofibrous surface.

scholarly journals Real‐Time In Vivo Detection of Cellular Senescence through the Controlled Release of the NIR Fluorescent Dye Nile Blue

2020 ◽  
Vol 132 (35) ◽  
pp. 15264-15268
Author(s):  
Beatriz Lozano‐Torres ◽  
Juan F. Blandez ◽  
Irene Galiana ◽  
Alba García‐Fernández ◽  
María Alfonso ◽  
...  
Keyword(s):  
Controlled Release ◽  
Real Time ◽  
Cellular Senescence ◽  
Nile Blue ◽  
Fluorescent Dye ◽  
In Vivo Detection
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