Hepatoprotective Activity of Linalool in Rats Against Liver Injury Induced by Carbon Tetrachloride

2020 ◽  
Vol 90 (3-4) ◽  
pp. 302-308 ◽  
Author(s):  
Fulya Altınok-Yipel ◽  
İbrahim Ozan Tekeli ◽  
Şule Yurdagül Özsoy ◽  
Mehmet Güvenç ◽  
Alpaslan Kaya ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Chemical Composition ◽  
Histopathological Examination ◽  
Hepatoprotective Activity ◽  
Normal Control Group ◽  
Control Group ◽  
Albino Rats ◽  
Antioxidant Parameters ◽  
Wistar Albino Rats ◽  
Group 1

Abstract. This study aimed to investigate and compare hepatoprotective activity of Coriandrum sativum (Cs) and it is major component linalool (Ln) against experimentally induced hepatotoxicity in rats. Essential oil of Cs was isolated by hydrodistillation method and chemical composition was determined by GS-MS analysis. 42 male Wistar Albino rats were divited into 7 groups each containing 6. The experimental groups were designed as: Normal control group, 1 ml/kg CCl4administirated group, 25 mg/kg Silymarin and CCl4administirated group, 100 and 200 mg/kg Cs and CCl4administirated groups, 100 and 200 mg/kg Ln and CCl4administered groups. The protective activities were determined according to the results of liver biomarkers (AST, ALT, ALP), antioxidant parameters (GSH, GPx, CAT), lipid peroxidation (MDA) and histopathological examination. Linalool percentage of Cs was 81.6%. The groups treated with linalool (100 and 200 mg/kg) (p < 0.01) and coriander (200 mg/kg) (p < 0.05) had significantly reduced AST (262–375) and ALT (101–290) levels (U/L) compared to the CCl4(600–622) group. The levels (nmol/g protein) of MDA (11–12) were significantly lower (p < 0.01), the levels of GSH (11–12) and the activities of CAT (23–24) were significantly higher (p < 0.01) in linalool groups (100 and 200 mg/kg) compared to the CCl4(18-5-10 respectively) group. These results were also supported by histopathological findings and indicate that Cs and Ln shows hepatoprotective activity against liver damage. In this regard, evaluation of activities of major components are needed to compare to medicinal plants in experimental diseases models.

scholarly journals In vivo Antioxidant Effects of Coconut (Cocos nucifera) Water Extract in Wistar Albino Rats

2020 ◽  
pp. 28-35
Author(s):  
O. J. Mba ◽  
U. I. Edward ◽  
O. A. Aja ◽  
I. E. Atiaetuk ◽  
M. K. Ndukwe
Keyword(s):  
Normal Control ◽  
Cocos Nucifera ◽  
Water Extract ◽  
Normal Control Group ◽  
Coconut Water ◽  
Control Group ◽  
Albino Rats ◽  
Wistar Albino Rats ◽  
Control Group Group ◽  
Group 1

Aim: The present study was designed to investigate the invivo antioxidant effects of coconut (Cocos nucifera) water extract in wistar albino rats. Methodology: Thirty (30) male wistar albino rats of mean weight 128 g were used for the study. The animals for the study were grouped into five (5) of six (6) rats each. Group 1 served as the normal control group that received feed and water only while groups 2, 3, 4, and 5 served as the test groups that were orally given 10 ml, 20 ml, 30 ml, and 40 ml of the coconut water extract for 28 days. The rats were sacrificed after 28 days and the blood samples were collected for biochemical analysis.  Results: From the result obtained there is a significant increase (p< 0.05) between the normal control group (group 1) and the test group (group 2) that received 20 ml of the coconut water extract for MDA. There is a significant increase (p< 0.05) between the normal control group (group 1) and the test groups (groups 2 and 5) that received 10 ml and 50 ml of the coconut water extract for SOD. Also, there is a significant increase (p< 0.05) between the normal control group (group 1) and the test groups (groups 2, 3 and 4) that received 20 ml, 30 ml and 40 ml of the coconut water extract for Catalase. For GSH and Vitamin C, there is a significant increase (p< 0.05) between the normal control group (group 1) and the test groups (groups 2, 3, 4 and 5) that were orally given 10 ml, 20 ml, 30 ml and 40 ml of the coconut water extract. Conclusion: The present investigation showed that the coconut water extract increased antioxidant properties in wistar albino rats and may also be used pharmacologically in the treatment of diseases implicated by free radicals.

Effects of erdosteine on cyclosporin-A-induced nephrotoxicity

2011 ◽  
Vol 31 (6) ◽  
pp. 565-573 ◽  
Author(s):  
M Tutanc ◽  
V Arica ◽  
N Yılmaz ◽  
A Nacar ◽  
I Zararsiz ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cyclosporin A ◽  
Protective Role ◽  
Control Group ◽  
Albino Rats ◽  
Protective Mechanism ◽  
Antioxidant Parameters ◽  
Group 2 ◽  
Wistar Albino Rats

Aim: In cyclosporin-A (CsA)-induced toxicity, oxidative stress has been implicated as a potential responsible mechanism. Therefore, we aimed to investigate the protective role of erdosteine against CsA-induced nephrotoxicity in terms of tissue oxidant/antioxidant parameters and light microscopy in rats. Materials and methods: Wistar albino rats were randomly separated into four groups. Group 1 rats treated with sodium chloride served as the control, group 2 rats were treated with CsA, group 3 with CsA plus erdosteine, and group 4 with erdosteine alone. Animals were killed and blood samples were analyzed for blood urea nitrogen (BUN), serum creatinine (Cr), uric acid (UA), total protein (TP), and albumin (ALB) levels. Kidney sections were analyzed for malondialdehyde (MDA) and nitric oxide (NO) levels and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities, as well as for histopathological changes. Results: In the CsA group, MDA, GSH-Px, BUN, and Cr levels were increased. The TP and ALB levels were decreased. These changes had been improved by erdosteine administration. Other biochemical parameters did not show any significant change. Conclusion: These results indicate that erdosteine produces a protective mechanism against CsA-induced nephrotoxicity and suggest a role of oxidative stress in pathogenesis.

Can curcumin modulate allergic rhinitis in rats?

2016 ◽  
Vol 130 (12) ◽  
pp. 1103-1109 ◽  
Author(s):  
M Acar ◽  
N Bayar Muluk ◽  
S Yigitaslan ◽  
B P Cengiz ◽  
P Shojaolsadati ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Goblet Cell ◽  
Histopathological Examination ◽  
Nasal Congestion ◽  
Control Group ◽  
Albino Rats ◽  
Epithelial Surface ◽  
Vascular Proliferation ◽  
Wistar Albino Rats ◽  
Goblet Cell Metaplasia

AbstractObjectives:This study aimed to explore the effects of curcumin on experimental allergic rhinitis in rats.Methods:Twenty-eight male Wistar albino rats were randomly divided into four groups: a control group; a group in which allergic rhinitis was induced and no treatment given; a group in which allergic rhinitis was induced followed by treatment with azelastine hydrochloride on days 21–28; and a group in which allergic rhinitis was induced followed by treatment with curcumin on days 21–28. Allergy symptoms and histopathological features of the nasal mucosa were examined.Results:The sneezing and nasal congestion scores were higher in the azelastine and curcumin treatment groups than in the control group. Histopathological examination showed focal goblet cell metaplasia on the epithelial surface in the azelastine group. In the curcumin group, there was a decrease in goblet cell metaplasia in the epithelium, decreased inflammatory cell infiltration and vascular proliferation in the lamina propria.Conclusion:Curcumin is an effective treatment for experimentally induced allergic rhinitis in rats.

scholarly journals Neuro-protective role of seeds of Mucuna pruriens BEK and Mucuna monosperma DC in wistar albino rats

2016 ◽  
Vol 4 (6) ◽  
pp. 276-281
Author(s):  
Desai Yogesh Hareshchandra ◽  
◽  
Ravi Mundugaru ◽  
Shridhara Bairy T ◽  
S Ravikrishna ◽  
...  
Keyword(s):  
Granular Layer ◽  
Histopathological Examination ◽  
Neuromuscular Disorders ◽  
Brain Regions ◽  
Neuroprotective Activity ◽  
Mucuna Pruriens ◽  
Control Group ◽  
Albino Rats ◽  
Anti Oxidant ◽  
Wistar Albino Rats

Mucuna pruriens Bek. and Mucuna monosperma DC. seeds were extensively used in Ayurveda for neuromuscular disorders. The objective of present study was to screen the neuroprotective activity of the test drugs in wistar albino rats. The cerebral ischemia was induced by bilateral common carotid artery occlusion for 60 minutes followed by reperfusion. At the end of the experiments under anaesthesia animals were sacrificed and brain was removed. Anti oxidant and histopathological examination was carried out of the brain tissue. Both test drugs have been shown considerable anti oxidant activity in comparison to BCCAO control group. Histopathological examination revealed there is a decreased cellularity and predominance of immature neurons in the granular layer was observed in hypothalamus in control rats while the test drug has shown normal cytoarchitecture. The sections of hippocampus from Mucuna monosperma DC group exhibited decreased cellularity of the granular layer and vacuolization was observed and Mucuna pruriens Bek treated group exhibited almost normal cytoarchitecture. In conclusion the test drugs possess moderate anti oxidant and cellular integrity maintaining potential in different brain regions and hence it supports its therapeutic claim in neuromuscular disorders.

scholarly journals DOES THE INTRATYMPANIC APPLICATION OF MESNA PREVENT THE CHOLESTEATOMA? AN EXPERIMENTAL RATS STUDY

2021 ◽  
Author(s):  
BİLAL SİZER ◽  
Aylin Gül ◽  
Songül Karababa Demir
Keyword(s):  
Propylene Glycol ◽  
Histopathological Examination ◽  
Salt Water ◽  
Statistical Significance ◽  
Control Group ◽  
Albino Rats ◽  
Microscopic Evaluation ◽  
Wistar Albino Rats ◽  
The Right ◽  
Experimental Group

Purpose Studying the effect of Mesna on middle ear otitis media and cholesteatoma induced by propylene glycol on an experimental animal model. Methods The study was designed to consist of sixteen Wistar albino rats, their right ears being the control group and left ears being the experiment group. %50 propylene glycol, gentamicinsulfate and physiologic salt water were applied to the right ear and %50 propylene glycol, gentamicinsulfate and %20 Mesna were administered to the left ear through intratympanic injections on days 1, 3, 8, 15 and 21. The rats were sacrificed 45 days after the first injection and underwent histopathological examination. Results It was seen that cholesteatoma and fibrosis were less common in the experiment group in microscopic evaluation. A statistically significant decrease was observed when the average and maximum thicknesses of the tympanic membranes and the minimum thicknesses of the tympanic bulla of the control group and the experiment group were compared. (p< 0.05) Conclusion In the experimental cholesteatoma model created in rats, no statistical significance was observed, indicating that Mesna, which was applied intratympanically, completely prevented the formation of cholesteatoma. However, it was found that the prevalence of cholesteatoma formation was microscopically less in the experimental group.

scholarly journals Lipid Peroxidation Activity and Phytochemical Constituents of Extract of Groundnut Peels

2020 ◽  
pp. 16-22
Author(s):  
Eneh Frank Uchenna ◽  
Ezeigwe Obiajulu Christian ◽  
Omeje Martha
Keyword(s):  
Lipid Peroxidation ◽  
Thiobarbituric Acid ◽  
Assay Method ◽  
Control Group ◽  
Albino Rats ◽  
Phytochemical Analysis ◽  
Phytochemical Constituents ◽  
Wistar Albino Rats ◽  
Living Tissues ◽  
Peel Extract

Aims: This study was designed to evaluate the phytochemical constituents and lipid peroxidation activities of extracts of two species of groundnut peels (Var-Hypogaea-Big and Var-vulgaris-Small). Study Design: Twenty-four (24) male wistar albino rats where randomized into four (4) groups of six (6) rats each. Group A was the baseline, Groups B and C were given feed formulated with 1% groundnut peel extract of Var-hypogaea and Var-vulgaris respectively, group D is the control group fed with a standard feed. Results: The phytochemical analysis was carried out using standard methods. Lipid peroxidation was determined by the thiobarbituric acid-reacting substances (TBARS) assay method. The phytochemical analysis revealed that the extract from the groundnut peels of Var-hypogaea-big contain alkaloids, tannins, saponnins, terpenoid, flavonoids phlobotannin, and glycosides. Also, the groundnut peel extracts of Var-vulgaris-small reveal the presence of alkaloids, tannins, saponnins, terpenoid, flavonoids phlobotannin and glycosides. There was a significant (p<0.05) increase in the malondialdehyde level of the groups that were fed with the feed compounded with both species of the groundnut compared with the baseline and normal control. However, the malondialdehyde level of the group fed with var-vulgaris was higher than that of Var-hypogaea. Conclusion: These results suggest that the groundnut peel extract of Var-hypogaea and Var-vulgaris is capable of inducing lipid peroxidation in living tissues. The groundnut peel does not only serve as a covering to the groundnut seed but may also protect the seeds from the effect of pests since it exhibits lipid peroxidative activity.

scholarly journals Evaluation of Antioxidant and Wound Healing Effects of Alcoholic and Aqueous Extract ofOcimum sanctum Linnin Rats

2008 ◽  
Vol 5 (1) ◽  
pp. 95-101 ◽  
Author(s):  
Somashekar Shetty ◽  
Saraswati Udupa ◽  
Laxminarayana Udupa
Keyword(s):  
Wound Healing ◽  
Aqueous Extract ◽  
Antioxidant Properties ◽  
Ocimum Sanctum ◽  
Control Group ◽  
Albino Rats ◽  
Impaired Wound Healing ◽  
Wistar Albino Rats ◽  
Different Doses ◽  
Group 1

In recent years, oxidative stress and free radicals have been implicated in impaired wound healing.Ocimum sanctum(O. sanctum), a plant widely used in Ayurveda, possesses anti-inflammatory and antioxidant properties. The present study was undertaken to assess the potential of alcoholic and aqueous extracts in wound healing in Wistar albino rats. The rats were divided into five groups of six animals each. Group 1 is normal wounded control and the other four groups were treated with two different doses each of alcoholic and aqueous extract ofO. sanctum. The wound healing parameters were evaluated by using incision, excision and dead space wounds in extract-treated rats and controls. Both the doses of alcoholic and aqueous extract significantly increased wound breaking strength, hydroxyproline, hexuronic acid, hexosamines, superoxide dismutase, catalase, reduced glutathione and significantly decreased percentage of wound contraction and lipid peroxidation when compared with the control group. The results suggest thatO. sanctumhas antioxidant properties, which may be responsible and favorable for faster wound healing and this plant extract may be useful in the management of abnormal healing and hypertropic scars.

scholarly journals Cystain C and neuropeptid Y levels in brain tissues after experimental subarachnoid hemorrhage.

2014 ◽  
Vol 61 (4) ◽  
Author(s):  
Şerefden Açıkgöz ◽  
Murat Can ◽  
Berrak Güven ◽  
Nurullah Edebali ◽  
Figen Barut ◽  
...  
Keyword(s):  
Subarachnoid Hemorrhage ◽  
Brain Tissue ◽  
Cystatin C ◽  
Histopathological Examination ◽  
Neuronal Loss ◽  
Control Group ◽  
Albino Rats ◽  
Tissue Homogenates ◽  
Experimental Subarachnoid Hemorrhage ◽  
Wistar Albino Rats

The aim of this study was to investigate the changes in the levels of cystatin C, which protects neurodegeneration in the central nervous system with the inhibition of cysteine protease and by inducing autophagy in the pathogenesis of cerebral vasospasm and levels of vasoconstrictive neuropeptid Y (NPY) in the brain tissue homogenates of rat model of subarachnoid hemorrhage (SAH). Three experimental groups were used: Day 2 and Day 7 groups after SAH, and also a control group. There were seven Wistar albino rats in each group. SAH was accomplished by transclival basilar artery puncture. Rat cystatin C, rat NPY were determined with ELISA in brain tissue homogenates. Day 2 group showed significantly enhanced cystatin C values in comparision with the control group (P=0.048). NPY levels between the Day 2 and Day 7 groups and the control groups were not significantly different (P=0.315). In histopathological examination, there was less neuronal loss in the Day 2 group than in the Day 7 group. Regarding our results, it would be more valuable to measure NPY levels in specific brain areas. The increased cystatin C levels on the second day after SAH is probably a pathophysiologic mechanism to organize protease activity.

Hepatoprotective Activity of Calotropis gigantea Root Bark Experimental Liver Damage Induced by D-Galactosamine in Rats

1970 ◽  
Vol 1 (3) ◽  
pp. 281-286
Author(s):  
Pradeep Deshmukh ◽  
Tanaji Nandgude ◽  
Mahendra Singh Rathode ◽  
Anil Midha ◽  
Nitin Jaiswal
Keyword(s):  
Methyl Cellulose ◽  
Hepatoprotective Activity ◽  
Albino Rats ◽  
Root Bark ◽  
Significant Activity ◽  
Alcoholic Extract ◽  
Calotropis Gigantea ◽  
Wistar Albino Rats ◽  
Experimental Liver ◽  
Hepatoprotective Drug

The suspensions of alcoholic extract of root bark of the plant Calotropis gigantea in 0.6% carboxy methyl cellulose (CMC) were evaluated for hepatoprotective activity in Wistar albino rats by inducing hepatic injury with D-galactosamine (400 mg/kg). Alcoholic extract of root bark of the plant Calotropis gigantea at an oral dose of 200 mg/kg and 400 mg/kg exhibited a significant (P<0.001, P<0.01 and P<0.05) protection effect by normalizing the levels of aspartate amino transferase (ASAT/ GOT), alanine amino transferase (ALAT/GPT), alkaline phosphatase (ALP), total bilirubin (TB), lactate dehydrogenase (LDH), which were significantly (P<0.001) increased in rats by treatment with 400 mg/kg i.p. of D-galactosamine. Silymarin (25 mg/kg), a known hepatoprotective drug used for comparison exhibited significant activity (P<0.001).

Effect of Propolis-Supplemented Diet on Body Composition and Endocrine Function of Adipose Tissue

2020 ◽  
Vol 19 (2) ◽  
pp. 217-221
Author(s):  
Maria Jesús Lisbona-González ◽  
Candela Reyes-Botella ◽  
Esther Muñoz-Soto ◽  
Maria Victoria Olmedo-Gaya, ◽  
Jorge Moreno-Fernandez ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Body Composition ◽  
Adipose Tissue ◽  
Endocrine Function ◽  
Control Group ◽  
Albino Rats ◽  
Standard Diet ◽  
Reduced Body ◽  
Esterified Fatty Acids ◽  
Wistar Albino Rats

Adipose tissue is an endocrine organ and has central role in interaction with other organs or tissues while propolis can induce lipolysis. Therefore, the aim of this study is to provide detailed information about adipose tissue homeostasis modifications and body composition during propolis supplement consumption. Twenty male Wistar albino rats (8 weeks) were divided into two groups of 10 animals each and fed for 90 days with two different types of diets: standard for the control group (diet C) and standard diet + 2% propolis (diet P). Thyroid hormones did not show differences, while ghrelin and adiponectin decreased in the group that was fed propolis. Insulin, leptin, and non-esterified fatty acids also increased along with reduced body weight and fat, in addition to increased lean mass when propolis was in the diet. We conclude that propolis could decrease ghrelin and adiponectin but increase non-esterified fatty acids and insulin secretion, which improves body composition.

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