scholarly journals Neuro-protective role of seeds of Mucuna pruriens BEK and Mucuna monosperma DC in wistar albino rats

2016 ◽  
Vol 4 (6) ◽  
pp. 276-281
Author(s):  
Desai Yogesh Hareshchandra ◽  
◽  
Ravi Mundugaru ◽  
Shridhara Bairy T ◽  
S Ravikrishna ◽  
...  
Keyword(s):  
Granular Layer ◽  
Histopathological Examination ◽  
Neuromuscular Disorders ◽  
Brain Regions ◽  
Neuroprotective Activity ◽  
Mucuna Pruriens ◽  
Control Group ◽  
Albino Rats ◽  
Anti Oxidant ◽  
Wistar Albino Rats

Mucuna pruriens Bek. and Mucuna monosperma DC. seeds were extensively used in Ayurveda for neuromuscular disorders. The objective of present study was to screen the neuroprotective activity of the test drugs in wistar albino rats. The cerebral ischemia was induced by bilateral common carotid artery occlusion for 60 minutes followed by reperfusion. At the end of the experiments under anaesthesia animals were sacrificed and brain was removed. Anti oxidant and histopathological examination was carried out of the brain tissue. Both test drugs have been shown considerable anti oxidant activity in comparison to BCCAO control group. Histopathological examination revealed there is a decreased cellularity and predominance of immature neurons in the granular layer was observed in hypothalamus in control rats while the test drug has shown normal cytoarchitecture. The sections of hippocampus from Mucuna monosperma DC group exhibited decreased cellularity of the granular layer and vacuolization was observed and Mucuna pruriens Bek treated group exhibited almost normal cytoarchitecture. In conclusion the test drugs possess moderate anti oxidant and cellular integrity maintaining potential in different brain regions and hence it supports its therapeutic claim in neuromuscular disorders.

Can curcumin modulate allergic rhinitis in rats?

2016 ◽  
Vol 130 (12) ◽  
pp. 1103-1109 ◽  
Author(s):  
M Acar ◽  
N Bayar Muluk ◽  
S Yigitaslan ◽  
B P Cengiz ◽  
P Shojaolsadati ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Goblet Cell ◽  
Histopathological Examination ◽  
Nasal Congestion ◽  
Control Group ◽  
Albino Rats ◽  
Epithelial Surface ◽  
Vascular Proliferation ◽  
Wistar Albino Rats ◽  
Goblet Cell Metaplasia

AbstractObjectives:This study aimed to explore the effects of curcumin on experimental allergic rhinitis in rats.Methods:Twenty-eight male Wistar albino rats were randomly divided into four groups: a control group; a group in which allergic rhinitis was induced and no treatment given; a group in which allergic rhinitis was induced followed by treatment with azelastine hydrochloride on days 21–28; and a group in which allergic rhinitis was induced followed by treatment with curcumin on days 21–28. Allergy symptoms and histopathological features of the nasal mucosa were examined.Results:The sneezing and nasal congestion scores were higher in the azelastine and curcumin treatment groups than in the control group. Histopathological examination showed focal goblet cell metaplasia on the epithelial surface in the azelastine group. In the curcumin group, there was a decrease in goblet cell metaplasia in the epithelium, decreased inflammatory cell infiltration and vascular proliferation in the lamina propria.Conclusion:Curcumin is an effective treatment for experimentally induced allergic rhinitis in rats.

scholarly journals DOES THE INTRATYMPANIC APPLICATION OF MESNA PREVENT THE CHOLESTEATOMA? AN EXPERIMENTAL RATS STUDY

2021 ◽  
Author(s):  
BİLAL SİZER ◽  
Aylin Gül ◽  
Songül Karababa Demir
Keyword(s):  
Propylene Glycol ◽  
Histopathological Examination ◽  
Salt Water ◽  
Statistical Significance ◽  
Control Group ◽  
Albino Rats ◽  
Microscopic Evaluation ◽  
Wistar Albino Rats ◽  
The Right ◽  
Experimental Group

Purpose Studying the effect of Mesna on middle ear otitis media and cholesteatoma induced by propylene glycol on an experimental animal model. Methods The study was designed to consist of sixteen Wistar albino rats, their right ears being the control group and left ears being the experiment group. %50 propylene glycol, gentamicinsulfate and physiologic salt water were applied to the right ear and %50 propylene glycol, gentamicinsulfate and %20 Mesna were administered to the left ear through intratympanic injections on days 1, 3, 8, 15 and 21. The rats were sacrificed 45 days after the first injection and underwent histopathological examination. Results It was seen that cholesteatoma and fibrosis were less common in the experiment group in microscopic evaluation. A statistically significant decrease was observed when the average and maximum thicknesses of the tympanic membranes and the minimum thicknesses of the tympanic bulla of the control group and the experiment group were compared. (p< 0.05) Conclusion In the experimental cholesteatoma model created in rats, no statistical significance was observed, indicating that Mesna, which was applied intratympanically, completely prevented the formation of cholesteatoma. However, it was found that the prevalence of cholesteatoma formation was microscopically less in the experimental group.

scholarly journals Evaluation of neuro-protective activity of Brihatvata Chinthamani Rasa

2015 ◽  
Vol 4 (4) ◽  
pp. 207-211
Author(s):  
Vikram Goshan ◽  
◽  
Ravi Mundugaru ◽  
Narayana Prakash ◽  
Sudhakar Bhat ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Drug Administration ◽  
Soft Tissues ◽  
Histopathological Examination ◽  
Brain Regions ◽  
Control Group ◽  
Test Drug ◽  
Anti Oxidant ◽  
Common Carotid Arteries

The objective of the study was to screen the neuroprotective role of Brihatvata Chinthamani Rasa, a compound mineral formulation extensively used in stroke condition in the Indian system of medicine. The selected animals were grouped into four different categories and administered with group specific drugs for seven consecutive days. On seventh day an hour after drug administration all the rats were anesthetised. A midline incision was made and soft tissues were pulled apart and both bilateral common carotid arteries were exposed. The cerebral ischemia were induced by simultaneous occlusion of both common carotid artery for 30min, followed by acute ischemic reperfusion injury was produced by untying the temporary ligature and releasing the thread. At the end of the experiment, under anaesthesia animals were sacrificed and brain has been removed. Anti oxidant and histopathological investigation were carried out of brain tissue. Control group rats have shown increased lipid peroxidation and decreased levels of catalase and glutathione peroxidase activity, while the test drug administration significantly elevated catalase and considerable decrease in the lipid peroxidation in comparison to control group. Histopathological examination revealed there is a decreased cellularity and predominance of immature neurons in the granular layer was observed in hypothalamus in control rats while the test drug has shown normal cytoarchitecture. In conclusion the test drug possesses storing anti oxidant and retained normal cellular integrity of different brain regions.

Hepatoprotective Activity of Linalool in Rats Against Liver Injury Induced by Carbon Tetrachloride

2020 ◽  
Vol 90 (3-4) ◽  
pp. 302-308 ◽  
Author(s):  
Fulya Altınok-Yipel ◽  
İbrahim Ozan Tekeli ◽  
Şule Yurdagül Özsoy ◽  
Mehmet Güvenç ◽  
Alpaslan Kaya ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Chemical Composition ◽  
Histopathological Examination ◽  
Hepatoprotective Activity ◽  
Normal Control Group ◽  
Control Group ◽  
Albino Rats ◽  
Antioxidant Parameters ◽  
Wistar Albino Rats ◽  
Group 1

Abstract. This study aimed to investigate and compare hepatoprotective activity of Coriandrum sativum (Cs) and it is major component linalool (Ln) against experimentally induced hepatotoxicity in rats. Essential oil of Cs was isolated by hydrodistillation method and chemical composition was determined by GS-MS analysis. 42 male Wistar Albino rats were divited into 7 groups each containing 6. The experimental groups were designed as: Normal control group, 1 ml/kg CCl4administirated group, 25 mg/kg Silymarin and CCl4administirated group, 100 and 200 mg/kg Cs and CCl4administirated groups, 100 and 200 mg/kg Ln and CCl4administered groups. The protective activities were determined according to the results of liver biomarkers (AST, ALT, ALP), antioxidant parameters (GSH, GPx, CAT), lipid peroxidation (MDA) and histopathological examination. Linalool percentage of Cs was 81.6%. The groups treated with linalool (100 and 200 mg/kg) (p < 0.01) and coriander (200 mg/kg) (p < 0.05) had significantly reduced AST (262–375) and ALT (101–290) levels (U/L) compared to the CCl4(600–622) group. The levels (nmol/g protein) of MDA (11–12) were significantly lower (p < 0.01), the levels of GSH (11–12) and the activities of CAT (23–24) were significantly higher (p < 0.01) in linalool groups (100 and 200 mg/kg) compared to the CCl4(18-5-10 respectively) group. These results were also supported by histopathological findings and indicate that Cs and Ln shows hepatoprotective activity against liver damage. In this regard, evaluation of activities of major components are needed to compare to medicinal plants in experimental diseases models.

scholarly journals Cystain C and neuropeptid Y levels in brain tissues after experimental subarachnoid hemorrhage.

2014 ◽  
Vol 61 (4) ◽  
Author(s):  
Şerefden Açıkgöz ◽  
Murat Can ◽  
Berrak Güven ◽  
Nurullah Edebali ◽  
Figen Barut ◽  
...  
Keyword(s):  
Subarachnoid Hemorrhage ◽  
Brain Tissue ◽  
Cystatin C ◽  
Histopathological Examination ◽  
Neuronal Loss ◽  
Control Group ◽  
Albino Rats ◽  
Tissue Homogenates ◽  
Experimental Subarachnoid Hemorrhage ◽  
Wistar Albino Rats

The aim of this study was to investigate the changes in the levels of cystatin C, which protects neurodegeneration in the central nervous system with the inhibition of cysteine protease and by inducing autophagy in the pathogenesis of cerebral vasospasm and levels of vasoconstrictive neuropeptid Y (NPY) in the brain tissue homogenates of rat model of subarachnoid hemorrhage (SAH). Three experimental groups were used: Day 2 and Day 7 groups after SAH, and also a control group. There were seven Wistar albino rats in each group. SAH was accomplished by transclival basilar artery puncture. Rat cystatin C, rat NPY were determined with ELISA in brain tissue homogenates. Day 2 group showed significantly enhanced cystatin C values in comparision with the control group (P=0.048). NPY levels between the Day 2 and Day 7 groups and the control groups were not significantly different (P=0.315). In histopathological examination, there was less neuronal loss in the Day 2 group than in the Day 7 group. Regarding our results, it would be more valuable to measure NPY levels in specific brain areas. The increased cystatin C levels on the second day after SAH is probably a pathophysiologic mechanism to organize protease activity.

Effect of Propolis-Supplemented Diet on Body Composition and Endocrine Function of Adipose Tissue

2020 ◽  
Vol 19 (2) ◽  
pp. 217-221
Author(s):  
Maria Jesús Lisbona-González ◽  
Candela Reyes-Botella ◽  
Esther Muñoz-Soto ◽  
Maria Victoria Olmedo-Gaya, ◽  
Jorge Moreno-Fernandez ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Body Composition ◽  
Adipose Tissue ◽  
Endocrine Function ◽  
Control Group ◽  
Albino Rats ◽  
Standard Diet ◽  
Reduced Body ◽  
Esterified Fatty Acids ◽  
Wistar Albino Rats

Adipose tissue is an endocrine organ and has central role in interaction with other organs or tissues while propolis can induce lipolysis. Therefore, the aim of this study is to provide detailed information about adipose tissue homeostasis modifications and body composition during propolis supplement consumption. Twenty male Wistar albino rats (8 weeks) were divided into two groups of 10 animals each and fed for 90 days with two different types of diets: standard for the control group (diet C) and standard diet + 2% propolis (diet P). Thyroid hormones did not show differences, while ghrelin and adiponectin decreased in the group that was fed propolis. Insulin, leptin, and non-esterified fatty acids also increased along with reduced body weight and fat, in addition to increased lean mass when propolis was in the diet. We conclude that propolis could decrease ghrelin and adiponectin but increase non-esterified fatty acids and insulin secretion, which improves body composition.

Hypolipidemic effect of prepared Polyherbal formulations in Wistar albino rats

2021 ◽  
pp. 4314-4320
Author(s):  
Ranjan Kumar Giri ◽  
Sunil Kumar Kanungo ◽  
Saroj Kumar Patro ◽  
Minaketan Sahoo ◽  
Dibya Sundar Panda
Keyword(s):  
Elevated Serum ◽  
Density Lipoprotein ◽  
Lipid Lowering ◽  
Control Group ◽  
Albino Rats ◽  
Histopathological Study ◽  
Oral Glucose ◽  
Hypolipidemic Effect ◽  
Standard Drug ◽  
Wistar Albino Rats

Lipid lowering effect of polyherbal formulations using eight different plants was evaluated in triton and diet induced hyperlipidemic models of wistar albino rats. Formulations such as Tablet, Syrup and Suspension inhibited the elevation in serum cholesterol and triglyceride levels on Triton WR 1339 administration rats. The formulations at the same dose level significantly attenuated the elevated serum total cholesterol and triglycerides with an increase in high-density lipoprotein cholesterol in high-fat diet-induced hyperlipidemic rats. The standard drug Niacin showed slightly better effects. The treatment with herbal formulations produced 30-35 percentage improvement in oral glucose tolerance. Similarly all the formulations also reduced the elevated C-reactive protein which is a marker of Hyperlipidemia. In histopathological study it was found that treatment of polyherbal formulation significantly reduced the plaque size in aorta compared with HFD treated control group. The outcome of the study reveals the lipid lowering activity of polyherbal formulations in dyslipidaemic conditions by interfering with the biosynthesis of cholesterol and utilization of lipids.

Effects of erdosteine on cyclosporin-A-induced nephrotoxicity

2011 ◽  
Vol 31 (6) ◽  
pp. 565-573 ◽  
Author(s):  
M Tutanc ◽  
V Arica ◽  
N Yılmaz ◽  
A Nacar ◽  
I Zararsiz ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cyclosporin A ◽  
Protective Role ◽  
Control Group ◽  
Albino Rats ◽  
Protective Mechanism ◽  
Antioxidant Parameters ◽  
Group 2 ◽  
Wistar Albino Rats

Aim: In cyclosporin-A (CsA)-induced toxicity, oxidative stress has been implicated as a potential responsible mechanism. Therefore, we aimed to investigate the protective role of erdosteine against CsA-induced nephrotoxicity in terms of tissue oxidant/antioxidant parameters and light microscopy in rats. Materials and methods: Wistar albino rats were randomly separated into four groups. Group 1 rats treated with sodium chloride served as the control, group 2 rats were treated with CsA, group 3 with CsA plus erdosteine, and group 4 with erdosteine alone. Animals were killed and blood samples were analyzed for blood urea nitrogen (BUN), serum creatinine (Cr), uric acid (UA), total protein (TP), and albumin (ALB) levels. Kidney sections were analyzed for malondialdehyde (MDA) and nitric oxide (NO) levels and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities, as well as for histopathological changes. Results: In the CsA group, MDA, GSH-Px, BUN, and Cr levels were increased. The TP and ALB levels were decreased. These changes had been improved by erdosteine administration. Other biochemical parameters did not show any significant change. Conclusion: These results indicate that erdosteine produces a protective mechanism against CsA-induced nephrotoxicity and suggest a role of oxidative stress in pathogenesis.

scholarly journals Evaluation of antidiabetic activity of fruit of Coriandrum sativum. Linn methanolic extract in Streptozocin induced diabetic wistar Albino rats

2017 ◽  
Vol 7 (1) ◽  
pp. 121
Author(s):  
Ahmed S. K. ◽  
Chakrapani Cheekavolu ◽  
Sampath D. ◽  
Sunil M.
Keyword(s):  
Research Study ◽  
Diabetes Management ◽  
Methanolic Extract ◽  
Coriandrum Sativum ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Albino Rats ◽  
Citrate Buffer ◽  
Standard Drug ◽  
Wistar Albino Rats

Background: Diabetes prevalence is estimated to increase annually. Numerous people use traditional medicine, such as India also considered as the diabetic capital in the world. Diabetes is a metabolic disorder characterized by disturbances in lipid, carbohydrate and protein metabolism. The present study to evaluate the antidiabetic potential of coriandrum sativum. linn fruits methanolic extract in streptozocin induced diabetic wistar albino rats model.Methods: Diabetes induction in wistar albino rats by administration of streptozocin (50mg/kg, i.p.) in citrate buffer. 30 wistar albino rats were divided into 5 groups (A, B, C, D, E). Group A: served as normal control, whereas Group B: diabetic control, Group C, D methanolic coriandrum sativum Linn. fruits extract (CSFME) at a dose of 100, 200mg/kg orally, Group E was given standard drug Glibenclamide (0.5mg/kg) orally. All groups are administered for the period of 14 consecutive days and blood sugar levels was measured at regular intervals up to end of the study.Results: This present research study confirms that the test drug compound CSFME has sustained oral hypoglycaemic activity and statistically significant (p ≤0.05) and which is comparable with standard drug Glibenclamide.Conclusions: This research study confirms that the CSFME has antidiabetic activity against streptozocin induced wistar diabetic albino rats. It could be a novel antidiabetic agent and also a dietary adjunct in the type 2 diabetes management and its complication. Further studies are necessary required to confirm the antidiabetic activity of individual phytochemical compounds of Coriandrum sativum.

scholarly journals Effect of Curcuma longa (Turmeric) on serum creatine kinase-MB and troponin I in isoproterenol induced myocardial infarction in wistar albino rats

2018 ◽  
Vol 13 (2) ◽  
pp. 47-53
Author(s):  
Sharmin Nahar ◽  
Qazi Shamima Akhter
Keyword(s):  
Myocardial Infarction ◽  
Body Weight ◽  
Troponin I ◽  
Curcuma Longa ◽  
Heart Weight ◽  
Control Group ◽  
Albino Rats ◽  
Creatine Kinase Mb ◽  
The Mean ◽  
Wistar Albino Rats

Background: The prevalence of myocardial infarction (MI) is increasing day by day in Bangladesh due to socioeconomic transition. Spices and herbs are important source of remedy for various diseases in human. Curcuma longa suggested to be used as an indigenous medicine for the prevention and treatment of cardiovascular disease. Objective: To observe the effect of Curcuma longa in isoproterenol induced myocardial infarction in Wistar albino rats. Methods: This experimental study was carried out in the Department of Physiology, Dhaka Medical College, Dhaka during 2015. Twenty one Wistar albino male rats, weighing 100 to 150 g (initial body weight); aged 85 to 100 days were selected for the study. After acclimatization for 14 days, the rats were divided into BC (Baseline control group), ISP-TC (Isoproterenol treated control group) and CLP-ISPT (Curcuma longa pretreated and isoproterenol treated group). Each group consisted of 7 rats. After experiment, on the 10th day, final body weight was taken, rats were sacrificed and blood samples were collected from the heart. The heart was removed and weighed. Serum creatine kinase-MB (CK-MB) level was estimated by ELISA method and Troponin I (cTnI) level by AxSYM method. The statistical analysis was done by one way ANOVA and Bonferroni test as applicable. Results: In this study, the mean percent (%) change of body weight (p<0.01), mean serum CK-MB (p<0.001) and cTnI (p<0.001) levels were significantly higher but mean heart weight was non significantly higher in ISP-TC in comparison to those of BC. Again, the mean percent (%) change of body weight (p<0.01), mean heart weight (p<0.01), mean serum CK-MB (p<0.01) and cTnI (p<0.001) levels were significantly lower in CLP-ISPT than those of ISP-TC group. Conclusion: From the results, it can be concluded that Curcuma longa may have cardioprotective effect. J Bangladesh Soc Physiol. 2018, December; 13(2): 47-53

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