scholarly journals The NAC transcription factor ClNAC68 positively regulates sugar content and seed development in watermelon by repressing ClINV and ClGH3.6

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Jinfang Wang ◽  
Yanping Wang ◽  
Jie Zhang ◽  
Yi Ren ◽  
Maoying Li ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Seed Development ◽  
Fruit Ripening ◽  
Sugar Content ◽  
Solid Content ◽  
Soluble Solid Content ◽  
Nac Transcription Factors ◽  
C Terminus ◽  
Soluble Solid ◽  
Repression Domain

AbstractNAC (NAM, ATAF1/2, and CUC2) transcription factors play important roles in fruit ripening and quality. The watermelon genome encodes 80 NAC genes, and 21 of these NAC genes are highly expressed in both the flesh and vascular tissues. Among these genes, ClNAC68 expression was significantly higher in flesh than in rind. However, the intrinsic regulatory mechanism of ClNAC68 in fruit ripening and quality is still unknown. In this study, we found that ClNAC68 is a transcriptional repressor and that the repression domain is located in the C-terminus. Knockout of ClNAC68 by the CRISPR-Cas9 system decreased the soluble solid content and sucrose accumulation in mutant flesh. Development was delayed, germination was inhibited, and the IAA content was significantly decreased in mutant seeds. Transcriptome analysis showed that the invertase gene ClINV was the only gene involved in sucrose metabolism that was upregulated in mutant flesh, and expression of the indole-3-acetic acid-amido synthetase gene ClGH3.6 in the IAA signaling pathway was also induced in mutant seeds. EMSA and dual-luciferase assays showed that ClNAC68 directly bound to the promoters of ClINV and ClGH3.6 to repress their expression. These results indicated that ClNAC68 positively regulated sugar and IAA accumulation by repressing ClINV and ClGH3.6. Our findings provide new insights into the regulatory mechanisms by which NAC transcription factors affect fruit quality and seed development.

scholarly journals Physical properties of raspberry and orange flavoured fruit syrups

2021 ◽  
Author(s):  
Anikó Lambert-Meretei ◽  
Eszter Vozary ◽  
Timea Kaszab
Keyword(s):  
Refractive Index ◽  
Physical Properties ◽  
Orange Juice ◽  
Electrical Impedance ◽  
Sugar Content ◽  
Solid Content ◽  
Soluble Solid Content ◽  
Fruit Composition ◽  
Definite Effect ◽  
Soluble Solid

Abstract In the case of fruit syrups, consumers prefer thicker, deep-coloured ones because they remind them of good old homemade ones. Physical properties – as viscosity, colour, refractive index, soluble solid content (SSC), and electrical impedance - of fruit syrups flavoured with raspberry or orange juice were determined. The change of the measured physical properties was determined in three different experiments: 1. evaluating the effect of sugar and sweetener content 2. evaluating the effect of fruit juice composition and 3. evaluating the effect of sugar content on syrups containing raspberry or orange juice only. Variations in fruit and sugar content had a definite effect on the physical properties of squashes. Viscosity, SSC value, refractive index, and impedance magnitude increased with increasing sugar content and decreased with replacement of sugar with sweeteners although colour characteristics were defined mostly by the colorant added to the squash. Changes in the fruit composition of syrups could influence viscosity, refractive index, and SSC values but the magnitude of impedance showed little changes for all samples. The characteristics of squashes containing only raspberry or orange juice and different sugar content did not follow any trends, which emphasized that additives had a significant effect on the physical properties.

scholarly journals Nectarine Fruit Ripening and Quality Assessed Using the Index of Absorbance Difference (IAD)

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
E. Bonora ◽  
D. Stefanelli ◽  
G. Costa
Keyword(s):  
Fruit Quality ◽  
Fruit Ripening ◽  
Solid Content ◽  
Training System ◽  
Fruit Growth ◽  
Soluble Solid Content ◽  
Training Systems ◽  
Climacteric Fruit ◽  
Soluble Solid ◽  
Absorbance Difference

Consistency of fruit quality is extremely important in horticulture. Fruit growth and quality in nectarine are affected by fruit position in the canopy, related to the tree shape. The “open shaped” training systems, such as Tatura Trellis, improve fruit growth and quality. The Index of Absorbance Difference (IAD) is a new marker that characterizes climacteric fruit during ripening. A study on fruit ripening was performed by using theIADon nectarine to monitor fruit maturity stages of two cultivars trained as Tatura Trellis in Victoria, Australia. Fruit of cv “Summer Flare 34” (“SF34”) grown in different positions on the tree showed high ripening homogeneity. Fruit harvested at a similar ripening stage showed fruit firmness and soluble solid content homogeneity. Fruits from hand-thinned variety “Summer Flare 26” (“SF26”) were larger in size, had advanced ripening, and showed greater homogeneity. For “SF26”, a weak correlation betweenIADand SSC was observed. The experiment showed that the Tatura Trellis training system is characterized by high homogeneity of nectarine fruit when coupled with a proper management of fruit density. It also confirmed that theIADcould be used as new nondestructive maturity index for nectarine fruit quality assessment in the field.

Role of Phospholipase D Inhibitor in Regulating Expression of Senescencerelated Phospholipase D gene in Postharvest Longan Fruit

2019 ◽  
Vol 14 (7) ◽  
pp. 649-657 ◽  
Author(s):  
Li Li ◽  
Jiemin Li ◽  
Jian Sun ◽  
Ping Yi ◽  
Changbao Li ◽  
...  
Keyword(s):  
Phospholipase D ◽  
Ascorbic Acid Content ◽  
Titratable Acidity ◽  
Significant Inhibition ◽  
Solid Content ◽  
Total Soluble Solid ◽  
Total Phenolic ◽  
Soluble Solid Content ◽  
Soluble Solid ◽  
Pericarp Browning

Background: Phospholipase D (PLD)is closely related to browning and senescence of postharvest longan fruit. Objective: This study investigated the effects of 2-butanol (a PLD inhibitor) on the expression and regulation of PLD during storage of longan fruit at a low temperature. Methods: Senescence-related quality indices showed that the 2-butanol-treated fruit presented lower pericarp browning index, pulp breakdown index and total soluble solid value than the untreated fruit. Results: The fruit treated by 60 µL/L 2-butanol exhibited the strongest inhibition on senescence, which significantly delayed changes in weight, titratable acidity content, total soluble solid content and ascorbic acid content. This treatment maintained a high level of total phenolic content and caused significant inhibition on pericarp browning and pulp breakdown. Through ELISA method, 60 µL/L 2-butanol treatment also reduced PLD activity. Real-time RT-PCR (RT-qPCR) results showed that PLD mRNA expression level was inhibited by 60 µL/L 2-butanol within 15 days. Western-blotting results further confirmed the differential expression of PLD during storage, and a relatively higher expression for PLD protein was found in control compared to the 2-butanoltreated fruit during 15-d storage. Conclusion: These results provided a scientific basis and reference to further investigating postharvest longan quality maintenance by regulating the PLD gene expression.

scholarly journals Effect of Cross-Linked Alginate/Oil Nanoemulsion Coating on Cracking and Quality Parameters of Sweet Cherries

Foods ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 449
Author(s):  
Camilo Gutiérrez-Jara ◽  
Cristina Bilbao-Sainz ◽  
Tara McHugh ◽  
Bor-Sen Chiou ◽  
Tina Williams ◽  
...  
Keyword(s):  
Titratable Acidity ◽  
Quality Parameters ◽  
Solid Content ◽  
Postharvest Quality ◽  
Soluble Solid Content ◽  
Nutritional Values ◽  
Quality Properties ◽  
Vapor Transfer ◽  
Sweet Cherries ◽  
Soluble Solid

The cracking of sweet cherries causes significant crop losses. Sweet cherries (cv. Bing) were coated by electro-spraying with an edible nanoemulsion (NE) of alginate and soybean oil with or without a CaCl2 cross-linker to reduce cracking. Coated sweet cherries were stored at 4 °C for 28 d. The barrier and fruit quality properties and nutritional values of the coated cherries were evaluated and compared with those of uncoated sweet cherries. Sweet cherries coated with NE + CaCl2 increased cracking tolerance by 53% and increased firmness. However, coated sweet cherries exhibited a 10% increase in water loss after 28 d due to decreased resistance to water vapor transfer. Coated sweet cherries showed a higher soluble solid content, titratable acidity, antioxidant capacity, and total soluble phenolic content compared with uncoated sweet cherries. Therefore, the use of the NE + CaCl2 coating on sweet cherries can help reduce cracking and maintain their postharvest quality.

scholarly journals The Mater-Bi® biodegradable film for strawberry (Fragaria x ananassa Duch.) mulching: effects on fruit yield and quality

2016 ◽  
Vol 11 (3) ◽  
pp. 203 ◽  
Author(s):  
Luigi Morra ◽  
Maurizio Bilotto ◽  
Domenico Cerrato ◽  
Raffaella Coppola ◽  
Vincenzo Leone ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Titratable Acidity ◽  
Solid Content ◽  
Soluble Solid Content ◽  
Chemical Parameters ◽  
Biodegradable Film ◽  
Yield And Quality ◽  
Physical Chemical ◽  
Soluble Solid

Two trials in different agricultural farms were carried out from October 2014 to June 2015 with the aim to assess the advantages linked to the substitution of the low density polyethylene (LDPE) films for soil mulching with the Mater-Bi<sup>®</sup> biodegradable films in the strawberry cultivation under tunnel in Campania. Lifetime of biodegradable mulch and influence of type of mulch on the yield and the quality of cvs Sabrina and Fortuna were evaluated. Plants were cultivated on mulched, raised beds, high 40 cm from bottom soil. Mater-Bi<sup>®</sup> film was 20 <span>µ</span>m thick while LDPE film was 50 mm thick. The physical-chemical parameters (firmness, pH, total soluble solid content, titratable acidity and skin colour) and some bioactive compounds (total polyphenols, flavonoids, anthocyanins, antioxidant activity) of fruits were determined by three samplings effected in consecutive months (from March to May 2015) of the harvest cycle. Biodegradable film guaranteed an effective mulch along the whole strawberry cycle (9-10 months including the time of drawing up of film). Yields of cv Sabrina on LDPE was 18% higher than those on Mater-Bi<sup>®</sup> while the opposite was detected in cv Fortuna (+10%). The physical-chemical parameters of fruits were not modified by the mulches. The content of the bioactive compounds, instead, resulted, in each time of sampling, significantly higher in fruits picked on Mater-Bi<sup>®</sup> based film.

scholarly journals Evaluation of production conditions of tomato grafted with different tobacco rootstocks and determining nicotine content and quality of fruit

2020 ◽  
Vol 115 (2) ◽  
pp. 297
Author(s):  
Seda TUNÇAY ÇAĞATAY ◽  
Gülşah ÇALIK KOÇ ◽  
Fereshteh REZAEİ ◽  
Özlem DARCANSOY İŞERI ◽  
Feride İffet ŞAHIN ◽  
...  
Keyword(s):  
Fruit Size ◽  
Titratable Acidity ◽  
Solid Content ◽  
Soluble Solid Content ◽  
Quality Factors ◽  
Nicotine Content ◽  
Positive Effects ◽  
Tomato Yield ◽  
Significant Difference ◽  
Soluble Solid

<p>This study aimed to investigate the effects of grafting tomato on different tobacco rootstocks on quality factors and nicotine content. The commercial variety (Solanum lycopersicum ‘H2274’) (BIOTECH) of the tomato was used as the scion plant, and six different tobacco (Nicotiana tabacum L.) rootstocks were used: Taşova, Tekel, Muş, Samsun, Dişbudak, Hasankeyf cultivars. Cleft grafting method was used in all plants. Yield of non-grafted and grafted plants grown in open-field conditions was calculated, and there was a significant increase in yield in grafted tomatoes compared to non-grafted tomatoes. There was significantly increased lycopene and β-carotene levels (mg kg-1), especially in ‘Tekel’, ‘Taşova’, ‘Samsun’, and ‘Hasankeyf’ tobacco grafts. There was a statistically significant difference between grafted and non-grafted plants according to 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical retention capacities and total phenol (TP) values. Evaluation of quality determinants including pH values, titratable acidity values (citric acid %), soluble solid content (SSC)(oBrix) , fruit size ratios, showed that tomatoes grafted with ‘Muş’ tobacco rootstock were of higher quality. There was no significant difference between grafted and non-grafted plants according to nicotine analysis of the tobacco-grafted tomatoes, and due to acceptable ranges of nicotin level on tobacco grafted tomato plants were considered to be suitable for consumption. It could be concluded that grafting practices have significantly positive effects on tomato yield and quality.<br /><br /></p>

scholarly journals Su Kısıtı Koşullarında Biber (Capsicum annuum L.) Bitkisinde Mikoriza Uygulamasının Vejetatif ve Generatif Gelişme Üzerinde Etkileri

2020 ◽  
Vol 8 (4) ◽  
pp. 927-931
Author(s):  
Kamile Ulukapı ◽  
Zehra Kurt ◽  
Sevinc Sener
Keyword(s):  
Plant Growth ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Solid Content ◽  
Healthy Plant ◽  
Soluble Solid Content ◽  
Glomus Etunicatum ◽  
Irrigation Level ◽  
Soluble Solid ◽  
Number Of Leaves

Arbuscular mycorrhizal fungi (AMF), which are beneficial soil organisms, have an important role in the uptake of plant nutrients by roots and thus help to healthy plant growth. The aim of this study was to determine the effects of AMF inoculation on the development of water-deficiency applied pepper plants. In this study, Tesla F1 pepper cultivars, Glomus etunicatum inoculated and without Glomus etunicatum, were exposed to four different irrigation regimes (25I, 50I, 75I, 100I). At the end of the experiment these plants were compared in terms of some vegetative and fruit properties. For this purpose, at the end of the trial; shoot length (cm), root length (cm), root spread (cm), number of leaves, leaf width and length (mm), stem diameter (mm), fruit width (mm), fruit length (mm), root and shoot weights (g), fruit pH, total soluble solid content and chlorophyll index were measured. P (phosphorus) and K (potassium) contents of leaves samples taken from plants were determined. As a result, it was determined that 75I irrigation regime gave the best results in terms of both plant growth and fruit properties in all mycorrhizal and non-mycorrhizal plants. It was also concluded that 75% irrigation level is sufficient for plant growth.

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