scholarly journals Biodegradation of selected hydrocarbons by novel bacterial strains isolated from contaminated Arabian Gulf sediment

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Assad Ahmed Al-Thukair ◽  
Karim Malik ◽  
Alexis Nzila
Keyword(s):  
Proteus Mirabilis ◽  
Arabian Gulf ◽  
Hydrocarbon Degradation ◽  
Contaminated Sediment ◽  
Bacterial Strains ◽  
Temperature Conditions ◽  
Naphthalene Degradation ◽  
Brevibacillus Brevis ◽  
Novel Bacteria

AbstractThree strains of novel bacteria were isolated from oil-contaminated sediment from the Arabian Gulf (Brevibacillus brevis T2C2008, Proteus mirabilis T2A12001, and Rhodococcus quinshengi TA13008). The isolated strains were tested for their degrading efficacy of low and high molecular hydrocarbon (naphthalene and pyrene). The efficacy of the two-hydrocarbon degradation by the isolates bacterial was determined at a temperature of 25 °C and 37 °C and pH of 5.0 and 9.0. In inoculated media at 37 °C, Rhodococcus qinshengi fully metabolized naphthalene and degrade 56% of pyrene. Brevibacillus brevis break down over 80% of naphthalene at room temperatures (25 °C). However, it was found that P. mirabilis and R. qinshengi biodegraded nearly 94% of naphthalene in the incubated media. The capacity for pyrene and naphthalene degradation in varying pH and temperature conditions was shown to be significant in Rhodococcus qinshengi because of its mineralization exceeding 50% across the tested pH and temperature. This implies that the isolated strains are ideal for biodegradation of contaminated sediment with naphthalene and pyrene.

scholarly journals Naphthalene degradation by bacterial strain VTPG5 Isolated from oil-contaminated sediment samples collected in Baria - Vungtau

2016 ◽  
Vol 14 (3) ◽  
pp. 573-579
Author(s):  
Lê Thị Nhi Công ◽  
Cung Thị Ngọc Mai ◽  
Vũ Ngọc Huy ◽  
Đỗ Văn Tuân
Keyword(s):  
Aromatic Hydrocarbons ◽  
Sole Source ◽  
Potential Method ◽  
Contaminated Sediment ◽  
Coastal Zones ◽  
Bacterial Strains ◽  
Sediment Samples ◽  
Naphthalene Degradation ◽  
Polluted Sites ◽  
Chemical Conditions

Naphthalene is one of the polycylclic aromatic hydrocarbons (PAHs) which cause environmental pollution leading to mutation and cancer to human. To transform and degrade naphthalene, a number of bioremediation approaches have been investigated. The fact that bacteria can utilize naphthalene as the sole source of carbon and energy was considered as high potential method to remove naphthalene. In this research, our purpose was to find highly degrading-naphthalene bacterial strains to apply in naphthalene removal from oil-polluted sites. Three bacterial strains which could well grow on naphthalene were isolated from oil contaminated sediment samples taken from coastal zones in Baria – Vungtau province. Among them, the Rhodococcus sp. VTPG5 was demonstrated to the best aromatic hydrocarbon degrader then it was selected for further investigation. The VTPG5 strain could grow with the concentration of 200 ppm and the best concentration for degrading capacity was 150 ppm. The optimal physio-chemical conditions for growth of VTPG5 were estimated at 37oC, pH 7 and concentration of NaCl 2.5% after 7 day-incubation. Using these conditions to incubation the VTPG5, by HPLC analysis it was confirmed that 99.9 % of naphthalene was degraded by VTPG5 strain with the initial concentration of 150 ppm after 7 days. The result may lead to the use of this strain in bioremediation technology to treat naphthalene and other PAH components containing in oil-polluted sediment samples.

scholarly journals Identification of biodegradation products of biphenyl and 2,3-dihydroxybiphenyl (2,3-DHB)

2014 ◽  
Vol 7 (1) ◽  
pp. 44-51 ◽  
Author(s):  
Slavomíra Murínová ◽  
Katarína Dercová ◽  
Peter Tarábek ◽  
Peter Tölgyessy
Keyword(s):  
Degradation Products ◽  
Pseudomonas Stutzeri ◽  
Dienoic Acid ◽  
Transformation Rate ◽  
Contaminated Sediment ◽  
Bacterial Strains ◽  
Fission Rate ◽  
Transformation Pathway ◽  
Biphenyl Degradation

Abstract We investigated the degradation of biphenyl and identified main degradation products. Biphenyl and 2,3-dihydroxybiphenyl (2,3-DHB) was added to cultivation media to identify whole collection of degradation products of four bacterial strains isolated from long-term PCB contaminated soil (Alcaligenes xylosoxidans and Pseudomonas stutzeri) and long-term PCB contaminated sediment (Ochrobactrum anthropi and Pseudomonas veronii). Cultivation flasks were processed in different time after inoculation to determine biphenyl fission rate. Alcaligenes xylosoxidans was revealed as the most appropriate strain for bioremediation process with the highest biphenyl transformation rate. Biphenyl degradation led to the formation of benzoic acid. However, as the presence of 2-hydroxy-6-oxo-6-phenylhex-2,4-dienoic acid (HOPDA) was not confirmed, the transformation pathway common for many other bacteria is probably modified.

Cross hybridization of plasmid and genomic DNA from aromatic and polycyclic aromatic hydrocarbon degrading bacteria

1991 ◽  
Vol 37 (12) ◽  
pp. 924-932 ◽  
Author(s):  
J. M. Foght ◽  
D. W. S. Westlake
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Genomic Dna ◽  
Bacterial Strains ◽  
Cross Hybridization ◽  
Pah Degradation ◽  
Naphthalene Degradation ◽  
Degrading Bacteria ◽  
Wide Range ◽  
Polycyclic Aromatic

Forty-three bacterial strains were collected from various environmental and commercial sources and their ability to degrade polycyclic aromatic hydrocarbons (PAHs) was confirmed using the criteria of growth, mineralization, and oxidation. Undigested genomic DNA from these strains was blotted by Southern transfer to replicate membranes, which were probed either with purified plasmids (e.g., TOL and NAH7, associated with toluene and naphthalene degradation, respectively) or with genomic DNA from the other strains. The isolates were grouped according to hybridization and PAH-degradation results. One group of eight strains grew on naphthalene vapors as sole carbon source and hybridized with archetypical NAH plasmids. Another group of six isolates mineralized phenanthrene but could not grow on naphthalene, and their cryptic plasmids hybridized with Pseudomonas sp. HL7b, which degrades a wide range of PAHs. The remaining isolates, which could not grow on naphthalene but mineralized and (or) oxidized a variety of PAHs, hybridized with neither the pure plasmids nor heterologous genomic DNA, implying that their PAH-degradative genes were significantly dissimilar. This suggests that using TOL or NAH plasmids to probe an environmental population might reveal toluene- or naphthalene-degradative genes but would underestimate the occurrence of PAH-degradative genes. We suggest that a suite of probes would be necessary to evaluate the PAH-degradation potential of a mixed population. Key words: polycyclic aromatic hydrocarbons, degradative plasmids, NAH plasmid, TOL plasmid, hybridization.

scholarly journals The Occurrence of blaCTX-M, blaSHV, and blaTEM Genes in Extended-Spectrum β-Lactamase-Positive Strains of Klebsiella pneumoniae, Escherichia coli, and Proteus mirabilis in Poland

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Dominika Ojdana ◽  
Paweł Sacha ◽  
Piotr Wieczorek ◽  
Sławomir Czaban ◽  
Anna Michalska ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistance ◽  
Klebsiella Pneumoniae ◽  
Proteus Mirabilis ◽  
Bacterial Strains ◽  
E Coli ◽  
Extended Spectrum ◽  
Genes Encoding

Bacteria belonging to the Enterobacteriaceae family that produce extended-spectrum β-lactamase (ESBL) enzymes are important pathogens of infections. Increasing numbers of ESBL-producing bacterial strains exhibiting multidrug resistance have been observed. The aim of the study was to evaluate the prevalence of blaCTX-M, blaSHV, and blaTEM genes among ESBL-producing Klebsiella pneumoniae, Escherichia coli, and Proteus mirabilis strains and to examine susceptibility to antibiotics of tested strains. In our study, thirty-six of the tested strains exhibited blaCTX-M genes (blaCTX-M-15, blaCTX-M-3, blaCTX-M-91, and blaCTX-M-89). Moreover, twelve ESBL-positive strains harbored blaSHV genes (blaSHV-18, blaSHV-7, blaSHV-2, and blaSHV-5), and the presence of a blaTEM gene (blaTEM-1) in twenty-five ESBL-positive strains was revealed. Among K. pneumoniae the multiple ESBL genotype composed of blaCTX-M-15, blaCTX-M-3, blaSHV-18, blaSHV-7, blaSHV-2, and blaSHV-5 genes encoding particular ESBL variants was observed. Analysis of bacterial susceptibility to antibiotics revealed that, among β-lactam antibiotics, the most effective against E. coli strains was meropenem (100%), whereas K. pneumoniae were completely susceptible to ertapenem and meropenem (100%), and P. mirabilis strains were susceptible to ertapenem (91.7%). Moreover, among non-β-lactam antibiotics, gentamicin showed the highest activity to E. coli (91.7%) and ciprofloxacin the highest to K. pneumoniae (83.3%). P. mirabilis revealed the highest susceptibility to amikacin (66.7%).

scholarly journals bla TEM, bla SHV and bla CTX-M-15 Extended Spectrum Beta-lactamase Produced by Acinetobacter baumanii, Enterobacter clocae and Proteus mirabilis from Pregnant Women in Three Secondary Health Care Facilities in South-south, Nigeria

2019 ◽  
pp. 1-9
Author(s):  
Florence Z. Uyanga ◽  
Emmanuel O. Ekundayo ◽  
Emmanuel O. Nwankwo
Keyword(s):  
Health Care ◽  
Pregnant Women ◽  
Acinetobacter Baumannii ◽  
Proteus Mirabilis ◽  
Bacterial Infections ◽  
Health Care Facilities ◽  
Bacterial Strains ◽  
Acinetobacter Baumanii ◽  
Care Facilities ◽  
Secondary Health Care

Background/Purpose: Based on fact there is high urinary tract infection and increasing treatment failure among pregnant women and this has led to increased mortality and morbidity among pregnant women, and increased stay in the hospital. This study was conducted to evaluate the prevalence of antimicrobial resistance and distribution of blaTEM, blaCTX-M-15 and blaSHV genes among A. baumannii, P. mirabilis and E. clocae strains isolated from urine samples from pregnant women attending antenatal at three secondary health care facilities south-south Nigeria. Methods: A. baumannii, P. mirabilis and E. clocae strains were isolated and identified using Microbact 24E. The disc diffusion and combined discs methods were used for testing antimicrobial susceptibility. The presence of ESBL was detected using Double Disk Synergy Test (DDST) and CHROMagar respectively. Plasmid extraction was carried out following the protocol of ZR Plasmid Miniprep-Classic extraction kit. Finally, the frequency of resistant genes including blaTEM, blaCTX-M-15 and blaSHV in selected 50 ESBL producing isolates was studied by PCR and using designed primers. Results: A total of 252 clinical isolates was collected from three secondary health care facilities in south-south, Nigeria. ESBLs were found in 231 (92%) isolates. blaCTX-M-15 was the commonest genotype (58.3%), followed by blaSHV (43.3%) and blaTEM (43.3%). Conclusion: ESBL positive strains of Enterobacter clocae, Acinetobacter baumannii and Proteus mirabilis are increasingly found in isolates from pregnant women. The widespread use of antibiotics has caused shifts in bacterial development to overcome the existing mechanisms of combating bacterial infections. These strains become resistant to frequently used antibiotics and they can pass the gene to other bacterial strains, the quick detection of these strains in clinical laboratories an essential step. The frequency of genes encoded ESBL isolates of Enterobacter clocae, Acinetobacter baumannii and Proteus mirabilis may be due to abuse and misuse of antibiotics.

scholarly journals Evaluation of Entomopathogenic Bacteria Against Pseudaulacaspis pentagona (Targioni-Tozzetti) (Hemiptera: Diaspididae)

2020 ◽  
Vol 8 (4) ◽  
pp. 424
Author(s):  
Elif Tozlu ◽  
Recep Kotan ◽  
Göksel Tozlu ◽  
Nasibe Tekiner ◽  
Önder Çalmaşur ◽  
...  
Keyword(s):  
Control Method ◽  
Alternative Methods ◽  
Effective Control ◽  
Bacterial Strains ◽  
Negative Effects ◽  
Controlled Conditions ◽  
Important Pest ◽  
Pseudaulacaspis Pentagona ◽  
Brevibacillus Brevis ◽  
White Peach Scale

Pseudaulacaspis pentagona (Targioni-Tozzetti) (Hemiptera: Diaspidae) which has a wide host range, is an important pest causing losses in yield. The insecticides are used for control against this pest but insecticidal control is difficult as scales protect themselves very effectively with hard, waxy armor. Also, the negative effects of the chemicals used in the control against benefical insects and with the increasing awareness on environmental issues, alternative methods were sought. Therefore, this study was performed to develop an alternative and effective control method for this pest by using biocontrol bacteria Bacillus pumilus (TV-67C), Brevibacillus brevis (CP-1) and Bacillus megaterium (TV-91C) under controlled conditions. The death adult number of the pest was recorded and mortality rate was calculated. All of the tested bacterial strains showed mortality rates from 41.68% to 89.04% against the white peach scale under controlled conditions. Consequently, our results indicated that especially B. pumilus strain TV-67C and B. brevis strain CP-1 can be used as biocontrol agents of P. pentagona.

scholarly journals Antibacterial substances of sponges, Agelas tubulata and Phyllospongia sp., from Manado Bay, against the growth of several bacterial strains

2019 ◽  
Vol 5 (1) ◽  
pp. 23
Author(s):  
Nani I.J Undap ◽  
Deiske A Sumilat ◽  
Robert Bara
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Proteus Mirabilis ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Active Fraction ◽  
Bacterial Strains ◽  
Agar Diffusion Method ◽  
Bahasa Indonesia

Title (Bahasa Indonesia): Senyawa antibakteri spons, Agelas tubulata dan Phyllospongia sp., dari Perairan Teluk Manado, terhadap pertumbuhan beberapa strain bakteri Agelas tubulataand Phyllospongiasp. are marine spongethat contain active compoundsthat have potential to beantibacterial, anticancer and antifungal which have not been used. This study was aimed to examine the sponge extracts which inhibitbacterial growth, i.e. Pseudomonas aeruginosa, Proteus mirabilis, Staphylococcus aureus, Escherichia coli, S. saprophyticus andtoanalyze the active fraction of the antibacterial component using bio-autographic method. Agelas tubulataand Phyllospongiasp. were collected from Manado Bayand method used in the study was Agar Diffusion Method. Results showed that A. tubulataand Phyllospongiasp. extract had antibacterial activity against all 5 bacteria tested. A. tubulataextract had inhibition zone withbroader spectrum than that of Phyllospongiasp. extract, while Phyllospongiasp. has specifity forP. mirabilis.Agelas tubulata danPhyllospongia sp. merupakan spesies spons laut yang mengandung senyawa aktif yang berpotensi antibakteri, antikanker dan antijamur yang belum banyak dimanfaatkan. Penelitian ini bertujuan untuk menguji ekstrak spons laut yang menghambat pertumbuhan 5 bakteri yaitu Pseudomonas aeruginosa, Proteus mirabilis, Staphylococcus aureus, Eschericia colidan Staphylococcus saprophyticus dan menganalisis fraksi aktif antibakteri melalui teknik bioautografi. Spesies spons laut A. tubulata dan Phyllospongiasp. diambil dari Perairan Pantai Malalayang Manado. Metode yang digunakan yaitu metode difusi agar. Hasil dari penelitian ini, yaitu ekstrak A. tubulatadan Phyllospongiasp. mempunyai aktivitas terhadap 5 bakteri uji. EkstrakA. tubulatamemiliki daya hambat dengan spektrum yang lebih luas dibandingkan ekstrak Phyllospongiasp.sedangkanekstrak Phyllospongia sp.memiliki spesifitas terhadap bakteri P. mirabilis.

scholarly journals Environmental Aspects of the Use of Hedera helix Extract in Bioremediation Process

2019 ◽  
Vol 7 (2) ◽  
pp. 43 ◽  
Author(s):  
Agata Zdarta ◽  
Wojciech Smułek ◽  
Amanda Pacholak ◽  
Ewa Kaczorek
Keyword(s):  
Cell Surface ◽  
Cell Surface Hydrophobicity ◽  
Acinetobacter Calcoaceticus ◽  
Surface Hydrophobicity ◽  
Hydrocarbon Degradation ◽  
Bacterial Strains ◽  
Environmental Aspects ◽  
Hedera Helix ◽  
Slowing Down ◽  
The Impact

This paper analyzes the impact of saponins from English ivy leaves on the properties of environmental bacterial strains and hydrocarbon degradation ability. For this purpose, two bacterial strains, Raoultella ornitinolytica M03 and Acinetobacter calcoaceticus M1B, have been used in toluene, 4-chlorotoluene, and α,α,α-trifluorotoluene biodegradation supported by Hedera helix extract. Moreover, theeffects of ivy exposition on cell properties and extract toxicity were investigated. The extract was found to cause minor differences in cell surface hydrophobicity, membrane permeability, and Zeta potential, although it adhered to the cell surface. Acinetobacter calcoaceticus M1B was more affected by the ivy extract; thus, the cells were more metabolically active and degraded saponins at greater amounts. Although the extract influenced positively the cells’ viability in the presence of hydrocarbons, it could have been used by the bacteria as a carbon source, thus slowing down hydrocarbon degradation. These results show that the use of ivy saponins for hydrocarbon remediation is environmentally acceptable but should be carefully analyzed to assess the efficiency of the selected saponins-rich extract in combination with selected bacterial strains.

Sign in / Sign up

Export Citation Format

Share Document