scholarly journals Novel Prosopis juliflora leaf ethanolic extract as natural antimicrobial agent against food spoiling microorganisms

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Iman Saleh ◽  
Mohammed H. Abu-Dieyeh
Keyword(s):  
Mycelial Growth ◽  
Ethanolic Extract ◽  
Geotrichum Candidum ◽  
Antimicrobial Activities ◽  
Prosopis Juliflora ◽  
Water Soluble ◽  
Small Scale ◽  
Health And Environmental Effects ◽  
Total Inhibition ◽  
Global Increase

AbstractFresh produces spoilage is a worldwide concern that accompany the global increase in food demand. Adverse human health and environmental effects of commercial spoilage control agents are major public concern. In this study, Prosopis juliflora leaves and fruit extracts had their antimicrobial activities evaluated against the growth of selected bacteria and yeast, and against mycelial growth and conidial germination of selected mycotoxins-producing fungi. P. juliflora water-soluble leaf ethanolic (PJ-WS-LE) extract with its novel extraction method showed the strongest antibacterial activity. Antimicrobial tests showed total inhibition of Botrytis cinerea, Alternaria alternata, Bacillus subtilis, Staphylococcus aureus and Candida albicans with MICs ranging between 0.125 and 1 mg/ml. Percent inhibition of mycelial growth (PIMG) of the extract was also determined against seven other fungal strains with highest value against Geotrichum candidum (66.2%). Even the least affected fungal strain showed alterations in their hyphae and spores exposed to PJ-WS-LE extract when observed using scanning electron microscope (SEM), alterations include exfoliated flakes, pores, vacuolation and applanation. Small-scale fruit bioassays controlled experiment showed high efficacy of the extract in protecting inoculated cherry tomato samples from B. cinerea and A. alternata infections. In conclusion, PJ-WS-LE extract is a feasible, natural antifungal agent that can replace common anti-spoiling chemicals.

The effect of nitrogen fertilizers on the ensiling characteristics of perennial ryegrass and cocksfoot

1970 ◽  
Vol 75 (3) ◽  
pp. 517-521 ◽  
Author(s):  
D. I. H. Jones
Keyword(s):  
Perennial Ryegrass ◽  
Matter Content ◽  
Buffering Capacity ◽  
N Fertilizer ◽  
Water Soluble ◽  
Nitrogen Fertilizers ◽  
Soluble Carbohydrates ◽  
Small Scale ◽  
Dry Matter Content ◽  
Growing Seasons

SUMMARYThe effect of three levels of N fertilizer on the ensiling characteristics of S. 24 perennial ryegrass and S. 37 cocksfoot have been examined during first growth in two growing seasons. The effects of sucrose supplementation, inoculation with Lactobacillus plantarum and wilting were also examined in certain cuts. All silages were made in the laboratory using a small scale vacuum silage technique.The perennial ryegrass herbage was higher in water soluble carbohydrates than the cocksfoot, N fertilizers decreased soluble carbohydrates and dry-matter content in both species. Buffering capacity was not consistently different between grasses or between N levels.Herbage was cut at two stages of maturity in the first year. In the first cut (8 days before ear emergence), perennial ryegrass silages were well preserved irrespective of the amount of N applied to the grass. Cocksfoot silages were well preserved only when the lowest level of N fertilizer had been applied (50 kg/ha). Supplementation of cocksfoot with sucrose prior to ensiling markedly improved silage quality, but inoculation had no effect. In the second cut (26 days after ear emergence) the grasses were higher in drymatter content and showed a lower buffering capacity, but neither ryegrass nor cocksfoot silages were well preserved unless supplemented with sucrose prior to ensiling.In the second year of the experiment only one cut was taken (9 days after ear emergence). As in the previous year, silages made from herbage at a late stage of growth were poorly preserved. Wilting prior to ensiling resulted in well-preserved silages.It is concluded that the need for additives and wilting to ensure satisfactory preservation varies in relation to the variety of grass used and its stage of growth.

Interlaboratory Validation of Small-Scale Solubility and Dissolution Measurements of Poorly Water-Soluble Drugs

2016 ◽  
Vol 105 (9) ◽  
pp. 2864-2872 ◽  
Author(s):  
Sara B.E. Andersson ◽  
Caroline Alvebratt ◽  
Jan Bevernage ◽  
Damien Bonneau ◽  
Claudia da Costa Mathews ◽  
...  
Keyword(s):  
Water Soluble ◽  
Small Scale ◽  
Poorly Water Soluble Drugs ◽  
Water Soluble Drugs ◽  
Interlaboratory Validation ◽  
Poorly Water Soluble

scholarly journals Chemical Analysis and Evaluation of Antioxidant, Antimicrobial, and Photoprotective Activities of Schinopsis brasiliensis Engl. (Anacardiaceae)

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Sarah Raquel Gomes de Lima-Saraiva ◽  
Fernanda Granja da Silva Oliveira ◽  
Raimundo Gonçalves de Oliveira Junior ◽  
Camila de Souza Araújo ◽  
Ana Paula de Oliveira ◽  
...  
Keyword(s):  
High Performance ◽  
Ethanolic Extract ◽  
Antimicrobial Activities ◽  
Physicochemical Analysis ◽  
Minimal Bactericidal Concentration ◽  
Native Plant ◽  
Protection Factor ◽  
Dpph Method ◽  
High Concentrations

Schinopsis brasiliensis Engl. is a native plant of Caatinga which has high concentrations of compounds capable of absorbing ultraviolet light, suggesting its potential application for the development of sunscreen preparations. After its identification and collection, this vegetable drug was submitted to a physicochemical analysis through the preparation of ethanolic extract. The phytochemical screening and analysis of extracts were carried out by high-performance liquid chromatography (HPLC) evaluation. The antioxidant activity of the extract was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and β-carotene bleaching test. Inhibitory hemolytic activity and morphological deformation of erythrocytes induced by H2O2 were also demonstrated and the antimicrobial activity was analyzed by the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) method. For the in vitro determination of the sun protection factor (SPF), the spectrophotometric method was used. From the analyses carried out with this species, this plant showed significant results for the antioxidant and antimicrobial activities, as well as sunscreen action. Important flavonoids were identified. These data are an important step for the development of new photoprotective cosmetic with Caatinga species, revealing importance and representing another incentive for the preservation of the species involved and analyzed in the study.

scholarly journals How big is the influence of biogenic silicon pools on short-term changes in water-soluble silicon in soils? Implications from a study of a 10-year-old soil–plant system

2017 ◽  
Vol 14 (22) ◽  
pp. 5239-5252 ◽  
Author(s):  
Daniel Puppe ◽  
Axel Höhn ◽  
Danuta Kaczorek ◽  
Manfred Wanner ◽  
Marc Wehrhan ◽  
...  
Keyword(s):  
Water Soluble ◽  
Small Scale ◽  
Testate Amoeba ◽  
Short Term ◽  
Plant Materials ◽  
Main Driver ◽  
Key Factor ◽  
Calamagrostis Epigejos ◽  
Biogenic Silicon ◽  
Future Work

Abstract. The significance of biogenic silicon (BSi) pools as a key factor for the control of Si fluxes from terrestrial to aquatic ecosystems has been recognized for decades. However, while most research has been focused on phytogenic Si pools, knowledge of other BSi pools is still limited. We hypothesized that different BSi pools influence short-term changes in the water-soluble Si fraction in soils to different extents. To test our hypothesis we took plant (Calamagrostis epigejos, Phragmites australis) and soil samples in an artificial catchment in a post-mining landscape in the state of Brandenburg, Germany. We quantified phytogenic (phytoliths), protistic (diatom frustules and testate amoeba shells) and zoogenic (sponge spicules) Si pools as well as Tiron-extractable and water-soluble Si fractions in soils at the beginning (t0) and after 10 years (t10) of ecosystem development. As expected the results of Tiron extraction showed that there are no consistent changes in the amorphous Si pool at Chicken Creek (Hühnerwasser) as early as after 10 years. In contrast to t0 we found increased water-soluble Si and BSi pools at t10; thus we concluded that BSi pools are the main driver of short-term changes in water-soluble Si. However, because total BSi represents only small proportions of water-soluble Si at t0 (< 2 %) and t10 (2.8–4.3 %) we further concluded that smaller (< 5 µm) and/or fragile phytogenic Si structures have the biggest impact on short-term changes in water-soluble Si. In this context, extracted phytoliths (> 5 µm) only amounted to about 16 % of total Si contents of plant materials of C. epigejos and P. australis at t10; thus about 84 % of small-scale and/or fragile phytogenic Si is not quantified by the used phytolith extraction method. Analyses of small-scale and fragile phytogenic Si structures are urgently needed in future work as they seem to represent the biggest and most reactive Si pool in soils. Thus they are the most important drivers of Si cycling in terrestrial biogeosystems.

scholarly journals Rizobactérias no controle de Sclerotinia sclerotiorum, e efeitos no desenvolvimento vegetativo de plântulas de soja

2020 ◽  
Vol 16 (4) ◽  
pp. 110-120
Author(s):  
Renata Silva Canuto de Pinho ◽  
Bruna Canabarro Pozzebon ◽  
Ketlen Raisa Rey Rodrigues ◽  
Renata Bolacel Arns ◽  
Cezario Almeida Alves ◽  
...  
Keyword(s):  
Volatile Compounds ◽  
Sclerotinia Sclerotiorum ◽  
Mycelial Growth ◽  
Water Soluble ◽  
Alternative Methods ◽  
Yield Potential ◽  
Vegetative Development ◽  
Produce Water ◽  
Soybean Seedlings ◽  
Root Size

Sclerotinia sclerotiorumis one of the main pathogens of soybean crop, reducing crop yield potential and causing losses of up to 37%. Because it is a soil inhabitant, its management is difficult. However, the adoption of alternative methods, such as the use of antagonists, may help reduce the pathogen inoculum. Thus, the objective of this work was to select native soybean rhizobacteria and to verify the potential of in vitroantagonism against Sclerotinia sclerotiorum, as well as the effect of these isolates on the germination and vegetative developmentof soybean seedlings. For this, tests of inhibition and mycelial growth rate index, production of volatile compounds, production of rhizobacterial metabolites and vegetative development of soybean seedlings were conducted.With respect to antagonism, I1, U4, M6, M8 and U13 isolates were the most effective. For the production of volatile compounds by the overlapping plate method, isolates M8, M10, M9, I1, M6 and U4 had the largest reductions in pathogen mycelial growth. For the ability of isolates to produce water-soluble metabolites in culture medium, it was found that isolates I1, M3, M6 and U13 were the most effective. For the vegetative development of soybean seedlings, except for the root length, in which the isolates M8, I14, M9, I1, M6 and M10 provided the largest increases in root size, the other variables did not show significant increases when compared with the witness.In general, isolates M8, M10, M9, I1, M6 and U4 are effective in inhibiting mycelial growth of Sclerotinia sclerotiorum, produce volatile organic compounds that help control the pathogen, however, they do not show significant increases in the vegetative development of soybean seedlings.

In Vitro Antimicrobial and Antioxidant Activities of Organic and Aqueous Extracts of Bacillus clausii KP10

2019 ◽  
Vol 41 (1) ◽  
pp. 161-161
Author(s):  
Farzana Nighat and Zahid Mushtaq Farzana Nighat and Zahid Mushtaq
Keyword(s):  
Hot Springs ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Aqueous Extracts ◽  
Current Case ◽  
Bacillus Clausii

Humans and plants are experiencing different kinds of diseases. Many of these diseases are mainly caused by microorganisms like fungi, bacteria and viruses that adversely affect their hosts. Bioactive compounds produced by bacteria can be used to treat the infections caused by these microorganisms. Free radicals that can cause serious damage to the cell can be repressed by antioxidants that are produced by bacteria as secondary compounds. The current case study was therefore planned to investigate the antimicrobial and antioxidant potentials of the organic and aqueous extracts of Bacillus clausii KP10 isolated recently by our group from hot springs of Tatta Pani, Poonch, Azad Kashmir. The antimicrobial activities were assessed using well diffusion method against two Gram positive bacteria; B. subtilis, and S. aureus, and two Gram negative bacteria; E. coli and P. multocida and three fungal strains; A. niger, A. parasiticus and A. ustus. Antioxidant activities of the extracts were done by DPPH free radical scavenging assay. In our experiments the maximum zones of inhibition were shown by ethanolic extract against P. multocida (21 mm), S. aureus (13 mm) and methanol soluble fraction showed zone of inhibition against A. ustus (21 mm) as compared to the standard ciprofloxacin for bacteria and as antifungal agents with terbinafine. In our studies n-hexane fraction of B. clausii KP10 showed maximum 88.67% antioxidant activity. Finally, results were analyzed statistically through analysis of variance (ANOVA). Hence it is proved that the bioactive fractions obtained from B. clausii KP10 are good source of antimicrobials and antioxidants and they can be used as medicines in future for the respective treatments.

scholarly journals Intrinsic Dissolution Rate Profiling of Poorly Water-Soluble Compounds in Biorelevant Dissolution Media

Pharmaceutics ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 493
Author(s):  
Alexandra Teleki ◽  
Olivia Nylander ◽  
Christel A.S. Bergström
Keyword(s):  
Dissolution Rate ◽  
Water Soluble ◽  
Small Scale ◽  
Intrinsic Dissolution ◽  
Intrinsic Dissolution Rate ◽  
Fed State ◽  
Pharmaceutical Ingredients ◽  
Biorelevant Dissolution ◽  
Intestinal Fluids ◽  
Simulated Intestinal Fluids

The intrinsic dissolution rate (IDR) of active pharmaceutical ingredients (API) is a key property that aids in early drug development, especially selecting formulation strategies to improve dissolution and thereby drug absorption in the intestine. Here, we developed a robust method for rapid, medium throughput screening of IDR and established the largest IDR dataset in open literature to date that can be used for pharmaceutical computational modeling. Eighteen compounds with diverse physicochemical properties were studied in both fasted and fed state simulated intestinal fluids. Dissolution profiles were measured in small-scale experimental assays using compound suspensions or discs. IDR measurements were not solely linked to API solubility in either dissolution media. Multivariate data analysis revealed that IDR strongly depends on compound partitioning into bile salt and phospholipid micelles in the simulated intestinal fluids, a process that in turn is governed by API lipophilicity, hydrophobicity, and ionization.

scholarly journals Comparison of the Nutrient Content of Eggs from Commercial and Village Chickens in Rural Malawi (P03-009-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Rochelle Werner ◽  
Bess Caswell ◽  
Kenneth Maleta ◽  
Christine Stewart
Keyword(s):  
Vitamin D3 ◽  
Large Scale ◽  
Nutrient Content ◽  
The United States ◽  
Water Soluble ◽  
Small Scale ◽  
Convenience Sample ◽  
Nutrient Analysis ◽  
The Mean ◽  
The Village

Abstract Objectives To characterize the nutritional composition of chicken eggs from a large-scale commercial producer and a small-scale household producer in rural Malawi. Methods A convenience sample of 28 large commercial and 32 village eggs from Malawi were hardboiled and measured for the weight in grams of the whole egg, peeled egg, egg white, and egg yolk. A separate convenience sample of 11 commercial and 17 village eggs were selected for nutrient analysis. Eggs were hardboiled for 4 minutes, refrigerated, and shipped to a nutrient analysis lab in the United States. Eggs from each source were pooled and analyzed for macronutrients, amino acids, fatty acids, vitamins, and minerals. Analytes were reported per 100 g sample and converted to nutrients per egg using the mean peeled egg weight. Results The mean weight in grams of whole commercial eggs (59.4 ± 5.3) was 19 g greater than whole village eggs (40.4 ± 3.0). Commercial eggs had a 15 g greater mass of egg whites (37.0 ± 4.2) than village eggs (21.8 ± 2.5), but the mass of egg yolks only differed by one gram (commercial: 15.3 ± 1.0 and village: 14.1 ± 1.4). Per 100 g sample, commercial and village eggs had similar calories (143 kcals vs. 162kcals), protein (12.5 g vs. 12.5 g), water-soluble vitamins (1.61 µg vs. 1.92 µg Vitamin B-12; 63.5 µg vs. 59.9 µg folate, DFE) and minerals (1.7 mg vs. 2.1 mg iron; 21 µg vs. 24 µg selenium; 1.1 mg vs. 1.4 mg zinc). For fat-soluble nutrients, the 100 g sample of commercial eggs had a higher concentration of Vitamin A than the village eggs (150 µg vs. 102 µg RAE) but lower concentrations of Vitamin D3, α-tocopherol, and choline than the village eggs (0.8 µg vs. 2.9 µg Vitamin D3; 2.25 mg vs. 4.08 mg α-tocopherol; and 238 mg vs. 314 mg choline). However, when compared on a per egg basis, the fat-soluble nutrient content of the whole eggs was similar due to the smaller size of the village eggs. Conclusions On a per egg basis, eggs from small-scale households may deliver comparable amounts of fat-soluble nutrients but fewer calories, protein, and minerals compared to eggs from commercial producers; however, on a per 100 g basis, village eggs were a more nutrient-dense option. Funding Sources The Bill and Melinda Gates Foundation, BLUM Center of UC Davis.

scholarly journals Large-Scale Production of Bioactive Terrein by Aspergillus terreus Strain S020 Isolated from the Saudi Coast of the Red Sea

Biomolecules ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 480 ◽  
Author(s):  
Hani Z. Asfour ◽  
Zuhier A. Awan ◽  
Alaa A. Bagalagel ◽  
Mahmoud A. Elfaky ◽  
Reda F. A. Abdelhameed ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Crude Extract ◽  
Large Scale ◽  
Aspergillus Terreus ◽  
Antimicrobial Activities ◽  
Carcinoma Cells ◽  
Small Scale ◽  
Scale Production ◽  
Fungal Strains

The diversity of symbiotic fungi derived from two marine sponges and sediment collected off Obhur, Jeddah (Saudi Arabia), was investigated in the current study. A total of 23 isolates were purified using a culture-dependent approach. Using the morphological properties combined with internal transcribed spacer-rDNA (ITS-rDNA) sequences, 23 fungal strains (in the majority Penicillium and Aspergillus) were identified from these samples. The biological screening (cytotoxic and antimicrobial activities) of small-scale cultures of these fungi yielded several target fungal strains which produced bioactive secondary metabolites. Amongst these isolates, the crude extract of Aspergillus terreus strain S020, which was cultured in fermentation static broth, 21 L, for 40 days at room temperature on potato dextrose broth, displayed strong antimicrobial activities against Pseudomonas aeruginosa and Staphylococcus aureus and significant antiproliferative effects on human carcinoma cells. Chromatographic separation of the crude extract by silica gel column chromatography indicated that the S020 isolate could produce a series of chemical compounds. Among these, pure crystalline terrein was separated with a high yield of 537.26 ± 23.42 g/kg extract, which represents the highest fermentation production of terrein to date. Its chemical structure was elucidated on the basis of high-resolution electrospray ionization mass spectrometry (HRESIMS) or high-resolution mass spectrometry (HRMS), 1D, and 2D NMR spectroscopic analyses and by comparison with reported data. The compound showed strong cytotoxic activity against colorectal carcinoma cells (HCT-116) and hepatocellular carcinoma cells (HepG2), with IC50 values of 12.13 and 22.53 µM, respectively. Our study highlights the potential of A. terreus strain S020 for the industrial production of bioactive terrein on a large scale and the importance of future investigations of these strains to identify the bioactive leads in these fungal extracts.

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