Intravenously Injected Human Fibroblasts Home to Skin Wounds, Deliver Type VII Collagen, and Promote Wound Healing

Nowadays, the number of chronic trauma cases caused by a variety of factors such as the world’s population-ageing and chronic diseases is increasing steadily, and thus effective treatment for chronic wounds has become a severe clinical challenge, which also burdens the patient both physically and financially. Therefore, it is urgent to develop new drugs to accelerate the healing of wounds. Bioactive peptides, which are relatively low cost, easy to produce, store and transport, have become an excellent choice. In this research, we identified a novel peptide OA-GL21, with an amino acid sequence of ‘GLLSGHYGRVVSTQSGHYGRG’, from the skin secretions of Odorrana andersonii. Our results showed that OA-GL21 exerted the ability to promote wound healing of human keratinocytes (HaCaT) and human fibroblasts in a dose- and time-denpendent manner. However, OA-GL21 had no significant effect on the proliferation of these two cells. Significantly, OA-GL21 showed obvious ability to promote wound healing in the full-thickness skin wound model in dose- and scar-free manners. Further studies showed that OA-GL21 had no direct antibacterial, hemolytic, and acute toxic activity; it had weak antioxidant activities but high stability. In conclusion, this research proved the promoting effects of OA-GL21 on cellular and animal wounds, and thus provided a new peptide template for the development of wound-repairing drugs.

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Effects of ALA-PDT on the Healing of Mouse Skin Wounds Infected With Pseudomonas aeruginosa and Its Related Mechanisms

Frontiers in Cell and Developmental Biology ◽

10.3389/fcell.2020.585132 ◽

2020 ◽

Vol 8 ◽

Author(s):

Tao Yang ◽

Yang Tan ◽

Wentao Zhang ◽

Weijiang Yang ◽

Jiefu Luo ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Wound Healing ◽

Photodynamic Therapy ◽

Aminolevulinic Acid ◽

Polarization State ◽

Mouse Skin ◽

Inflammatory Factors ◽

Therapeutic Effects ◽

Skin Wounds ◽

Promote Wound Healing

Photodynamic therapy (PDT) is a promising new method to eliminate microbial infection and promote wound healing. Its effectiveness has been confirmed by some studies; however, the mechanisms of PDT in wound healing remain obscure. We used mouse skin wounds infected with Pseudomonas aeruginosa as a research object to explore the therapeutic effects and mechanisms of 5-aminolevulinic acid photodynamic therapy (ALA-PDT). ALA-PDT treatment significantly reduced the load of P. aeruginosa in the wound and surrounding tissues and promoted the healing of skin wounds in mice. Hematoxylin-eosin (HE) and Sirius red staining showed that ALA-PDT promoted granulation tissue formation, angiogenesis, and collagen regeneration and remodeling. After ALA-PDT treatment, the expression of inflammatory factors (TNF-α and IL-1β) first increased and then decreased, while the secretion of growth factors (TGF-β-1 and VEGF) increased gradually after treatment. Furthermore, ALA-PDT affected the polarization state of macrophages, activating and promoting macrophages from an M1 to an M2 phenotype. In conclusion, ALA-PDT can not only kill bacteria but also promote wound healing by regulating inflammatory factors, collagen remodeling and macrophages. This study further clarifies the mechanism of PDT in the healing of infectious skin wounds and provides further experimental evidence for its clinical treatment of skin wounds infected by P. aeruginosa.

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Ultraviolet-A Light and Negative Pressure Wound Therapy to Accelerate Wound Healing and Reduce Bacterial Proliferation

Journal of the American Podiatric Medical Association ◽

10.7547/20-251 ◽

2021 ◽

Author(s):

Kathryn E Davis ◽

Jessica Bills ◽

Debby Noble ◽

Peter A Crisologo ◽

Lawrence A Lavery

Keyword(s):

Dna Damage ◽

Wound Healing ◽

Negative Pressure ◽

Negative Pressure Wound Therapy ◽

Fiber Optic ◽

Human Fibroblasts ◽

Ultraviolet A ◽

Wound Therapy ◽

Promote Wound Healing ◽

Bacterial Proliferation

Background: Ultraviolet-A therapy is a simple, inexpensive, and effective modality for wound healing with tremendous potential to improve healing and reduce clinical infections in several clinical settings. To date, application of UV-A relies on bulky and hard to dose lamps that provide inconsistent therapy, thus making it difficult to apply therapy that is appropriate for the patient. Methods: This study was designed to test the effectiveness of a novel wound therapy device that combines UV-A with traditional negative pressure wound therapy to promote wound healing. Further, we tested the ability of fiber optic UV-A delivery to inhibit bacterial proliferation. Finally, we assayed the level of DNA damage that results from UV-A as compared to established UV-C therapies. Wound healing studies were performed in a porcine model using an articulated therapy arm that allows for continued therapy administration over an extended time course. Negative pressure wound therapy was administered alone or with ultraviolet-A fiber optic therapy for 2 weeks. Dressings were changed twice a week at which time wound area was assessed. Results: Data demonstrate that UV-A with NPWT treatment of wounds results in greater healing than NPWT alone. Using the same therapy device, we demonstrate that exposure of Staphylococcus aureus and Pseudomonas aeruginosa to fiber optic UV-A results in decreased colony area and number of both bacterial strains. Finally, we show that UV-A induces minimal DNA damage in human fibroblasts and no more DNA damage in wound tissue as compare to intact skin. Conclusions: These data demonstrate that UV-A can decrease bacterial proliferation and promote wound healing when coupled with NPWT.

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Near-Infrared Laser-Excited Nanoparticles To Eradicate Multidrug-Resistant Bacteria and Promote Wound Healing

ACS Applied Materials & Interfaces ◽

10.1021/acsami.7b15251 ◽

2017 ◽

Vol 10 (1) ◽

pp. 193-206 ◽

Cited By ~ 40

Author(s):

Yue Qiao ◽

Fei Ma ◽

Chao Liu ◽

Bo Zhou ◽

Qiaolin Wei ◽

...

Keyword(s):

Wound Healing ◽

Near Infrared ◽

Multidrug Resistant ◽

Infrared Laser ◽

Resistant Bacteria ◽

Multidrug Resistant Bacteria ◽

Promote Wound Healing

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A pulsatile release platform based on photo-induced imine-crosslinking hydrogel promotes scarless wound healing

Nature Communications ◽

10.1038/s41467-021-21964-0 ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Jian Zhang ◽

Yongjun Zheng ◽

Jimmy Lee ◽

Jieyu Hua ◽

Shilong Li ◽

...

Keyword(s):

Wound Healing ◽

Wound Repair ◽

Transforming Growth Factor ◽

Transforming Growth Factor Β ◽

Skin Wound ◽

Large Animal ◽

Skin Wounds ◽

Tgfβ Signaling ◽

Pulsatile Release ◽

Ecm Extracellular Matrix

AbstractEffective healing of skin wounds is essential for our survival. Although skin has strong regenerative potential, dysfunctional and disfiguring scars can result from aberrant wound repair. Skin scarring involves excessive deposition and misalignment of ECM (extracellular matrix), increased cellularity, and chronic inflammation. Transforming growth factor-β (TGFβ) signaling exerts pleiotropic effects on wound healing by regulating cell proliferation, migration, ECM production, and the immune response. Although blocking TGFβ signaling can reduce tissue fibrosis and scarring, systemic inhibition of TGFβ can lead to significant side effects and inhibit wound re-epithelization. In this study, we develop a wound dressing material based on an integrated photo-crosslinking strategy and a microcapsule platform with pulsatile release of TGF-β inhibitor to achieve spatiotemporal specificity for skin wounds. The material enhances skin wound closure while effectively suppressing scar formation in murine skin wounds and large animal preclinical models. Our study presents a strategy for scarless wound repair.

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Systematic Review: Adipose-Derived Mesenchymal Stem Cells, Platelet-Rich Plasma and Biomaterials as New Regenerative Strategies in Chronic Skin Wounds and Soft Tissue Defects

International Journal of Molecular Sciences ◽

10.3390/ijms22041538 ◽

2021 ◽

Vol 22 (4) ◽

pp. 1538 ◽

Cited By ~ 2

Author(s):

Pietro Gentile ◽

Simone Garcovich

Keyword(s):

Systematic Review ◽

Stem Cells ◽

Wound Healing ◽

Soft Tissue ◽

Chronic Wounds ◽

Platelet Rich Plasma ◽

Skin Wounds ◽

Soft Tissue Defects ◽

Chronic Skin ◽

Tissue Defects

The number of clinical trials evaluating adipose-derived mesenchymal stem cells (AD-MSCs), platelet-rich plasma (PRP), and biomaterials efficacy in regenerative plastic surgery has exponentially increased during the last ten years. AD-MSCs are easily accessible from various fat depots and show intrinsic plasticity in giving rise to cell types involved in wound healing and angiogenesis. AD-MSCs have been used in the treatment of soft tissue defects and chronic wounds, employed in conjunction with a fat grafting technique or with dermal substitute scaffolds and platelet-rich plasma. In this systematic review, an overview of the current knowledge on this topic has been provided, based on existing studies and the authors’ experience. A multistep search of the PubMed, MEDLINE, Embase, PreMEDLINE, Ebase, CINAHL, PsycINFO, Clinicaltrials.gov, Scopus database, and Cochrane databases has been performed to identify papers on AD-MSCs, PRP, and biomaterials used in soft tissue defects and chronic wounds. Of the 2136 articles initially identified, 422 articles focusing on regenerative strategies in wound healing were selected and, consequently, only 278 articles apparently related to AD-MSC, PRP, and biomaterials were initially assessed for eligibility. Of these, 85 articles were excluded as pre-clinical, experimental, and in vitro studies. For the above-mentioned reasons, 193 articles were selected; of this amount, 121 letters, expert opinions, commentary, and editorials were removed. The remaining 72 articles, strictly regarding the use of AD-MSCs, PRP, and biomaterials in chronic skin wounds and soft tissue defects, were analyzed. The studies included had to match predetermined criteria according to the patients, intervention, comparator, outcomes, and study design (PICOS) approach. The information analyzed highlights the safety and efficacy of AD-MSCs, PRP, and biomaterials on soft tissue defects and chronic wounds, without major side effects.

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A moisture balanced antibacterial dressing loaded with lysozyme possesses antibacterial activity and promotes wound healing

Soft Matter ◽

10.1039/d0sm02245d ◽

2021 ◽

Vol 17 (11) ◽

pp. 3162-3173

Author(s):

Ling Xiao ◽

Wenqiang Ni ◽

Xiaohong Zhao ◽

Yicheng Guo ◽

Xue Li ◽

...

Keyword(s):

Wound Healing ◽

Antibacterial Activity ◽

Promote Wound Healing

An antibacterial moisture balanced dressing is designed to fight infection and promote wound healing.

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The Effect of a Hydrocolloid Dressing on Second Intention Wound Healing in Cats

Journal of the American Animal Hospital Association ◽

10.5326/jaaha-ms-6604 ◽

2018 ◽

Vol 54 (3) ◽

pp. 125-131 ◽

Cited By ~ 2

Author(s):

Vassiliki Tsioli ◽

Pagona G. Gouletsou ◽

Apostolos D. Galatos ◽

Dimitra Psalla ◽

Antonios Lymperis ◽

...

Keyword(s):

Wound Healing ◽

Clinical Evaluation ◽

The Other ◽

Skin Wounds ◽

Tissue Formation ◽

Dorsal Midline ◽

Granulation Tissue Formation ◽

Hydrocolloid Dressing ◽

Thickness Skin ◽

The Right

ABSTRACT The objective of the present study was to evaluate the effect of a hydrocolloid dressing on second intention wound healing in cats. Two full-thickness skin wounds, measuring 2 × 2 cm, were created on both sides of the dorsal midline of 10 cats; bilaterally, one randomly selected wound was bandaged with a hydrocolloid dressing and the second one (control) with a semiocclusive pad. Subjective clinical evaluation of granulation tissue formation, of the quantity and nature of wound exudate, and planimetry were performed on the right-side wounds, and histological examination on the left. No significant differences in subjective clinical evaluation or in planimetry were observed between the hydrocolloid-treated wounds and controls. Most wounds had serous or absence of exudate (41.25% and 25%, respectively), whereas purulent exudate was observed in 7.5% of wounds. Edema was significantly increased in the hydrocolloid-treated wounds compared with controls on day 7 but no significant differences in the other histological variables were observed.

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