Rapid detection and enumeration of total bacteria in drinking water and tea beverages using a laboratory-built high-sensitivity flow cytometer

2015 ◽  
Vol 7 (7) ◽  
pp. 3072-3079 ◽  
Author(s):  
Muxin Yu ◽  
Lina Wu ◽  
Tianxun Huang ◽  
Shuo Wang ◽  
Xiaomei Yan
Keyword(s):  
Drinking Water ◽  
Rapid Detection ◽  
High Sensitivity ◽  
Flow Cytometer ◽  
Total Bacteria

This paper describes a method for the rapid detection and enumeration of total bacteria in drinking water and tea beverages.

scholarly journals Sodium Hypochlorite Treatment: The Impact on Bacteria and Endotoxin Concentrations in Drinking Water Pipes of A Pig Nursery

Agriculture ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 86
Author(s):  
Regina Böger ◽  
Karl Rohn ◽  
Nicole Kemper ◽  
Jochen Schulz
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Sodium Hypochlorite ◽  
Coliform Bacteria ◽  
Treated Water ◽  
Water Pipes ◽  
Pseudomonas Spp ◽  
Untreated Water ◽  
The Impact ◽  
Total Bacteria

Poor drinking water quality can affect pigs’ health and performance. The disinfection of water may enhance microbial water quality. In this study, bacteria and endotoxins in sodium hypochlorite-treated and -untreated water from one pig nursery were analyzed. Water samples were taken from incoming water and from compartments with treated and untreated water at the beginning and end of pipes and from nipples. The farm was visited 14 times to measure total bacteria counts and concentrations of Pseudomonas spp. and endotoxins. Additionally, the occurrence of coliform bacteria was analyzed. A mixed model analysis revealed significant reductions in total bacteria counts and Pseudomonas spp. in treated water at the beginning of pipes and at nipple drinkers. The differences between bacteria concentrations at the end of pipes had no clear trend. Endotoxin concentrations were approximately equal at the beginning of pipes and at nipple drinkers but were found to have differences at the end of pipes. The occurrence of coliform bacteria was significantly reduced in treated water. The application of sodium hypochlorite can significantly reduce bacteria in water pipes. Endotoxin concentrations were mostly unaffected by water treatment. Disinfection of the dead-end pipe sections failed, and thus these parts should be regarded as potential contamination sources.

Gold Immunochromatography Assay - A Newly Rapid Detection Technique for Aquatic Products

2013 ◽  
Vol 690-693 ◽  
pp. 1449-1454
Author(s):  
Yuan Wang ◽  
Hui Juan Yu ◽  
Bei Lei Qian ◽  
You Qiong Cai ◽  
Dong Mei Huang ◽  
...  
Keyword(s):  
Rapid Detection ◽  
High Sensitivity ◽  
Detection Technique ◽  
Detection Methods ◽  
Future Perspectives ◽  
Auxiliary Equipment ◽  
Basic Principles ◽  
Aquatic Products ◽  
Immunological Detection

Gold immunochromatography assay (GICA) technique has the following characteristics: rapid and simple, high sensitivity, good specificity, no auxiliary equipment, ease of interpreting results, and satisfactory stability. The technique has become one of the most rapid and sensitive immunological detection methods, which is widely used in medical, biological and other fields. The article focuses on the basic principles and technical characteristics of GICA, and briefly describes the applications and future perspectives in the rapid detection of aquatic products.

New Perspectives for SARS-CoV-2 Rapid Detection

Coronaviruses ◽  
2021 ◽  
Vol 02 ◽  
Author(s):  
Latifa Khattabi ◽  
Mustapha Mounir Bouhenna ◽  
Feriel Sellam
Keyword(s):  
Rapid Detection ◽  
High Sensitivity ◽  
The Other ◽  
Host Cells ◽  
Rt Pcr ◽  
Human Immune System ◽  
Virus Attachment ◽  
Testing Procedures ◽  
Diagnostic Kits ◽  
Human Immune

: The present paper elucidates the conceivable application of two key molecules in SARS-CoV-2 detection of suspected infected persons. These molecules were selected from the basis of ACE-2 and S protein strong interaction that allows virus attachment to its host cells, on the other hand specific immunocompetant effectors generated by human immune system during the infection. Several testing procedures are already used to diagnose SARS-CoV-2 infection, particularly RT-PCR technique. ELISA and LFIA are possible assays for the employment of shACE-2/ hAc-anti-S (the molecules of interest) as the main agents of the test and confer a dual principal functions (capture and detection). The future diagnostic kits involving shACE-2 and hAc-anti-S will have the particularity of high sensitivity and rapid detection in addition to its advantage of relatively easy conception. It could be largely considered as a technical advanced kits in regards to the current SARS-CoV-2 diagnostic immunoassays.

The Study on Organic Acid Detection Using Lon Chromatography

2012 ◽  
Vol 424-425 ◽  
pp. 923-926
Author(s):  
Xiao Jian Hu ◽  
Wen Chen ◽  
Li Li ◽  
Jin Ping Zhou ◽  
Bao Shou Guo ◽  
...  
Keyword(s):  
Drinking Water ◽  
Convenient Method ◽  
High Sensitivity ◽  
Microwave Oven ◽  
Good Recovery ◽  
Microwave Technology ◽  
Trace Level ◽  
Recovery Treatment ◽  
Technology Foundation

A simple and convenient method for the determination of μ g/l level in the real drinking water bromate overcome traditional methods of defects. Argentina by electrolyzing the smallest electrode interference and then focus on extra chlorine use microwave technology foundation evaporation and concentration, the proposed method is very sensitive to the determination of trace level of the sales of the drinking water. And a microwave oven, drinking water ten times the sample can be concentrated in 10 minutes can get and good recovery. Treatment of the silver electrode, the method of interference, and can effectively eliminate the Cl- This method is successfully applied to the detection in water sample level in the trace. It is the advantage of the operation is simple, fast, high sensitivity, accurate results. This method can be applied widely, suitable for trace analysis of water samples.

An improved method for determining microbially available phosphorus in drinking water

2016 ◽  
Vol 16 (4) ◽  
pp. 1149-1158 ◽  
Author(s):  
Gang Wen ◽  
Qin Deng ◽  
Ting-Lin Huang ◽  
Jun Ma
Keyword(s):  
Drinking Water ◽  
Microbial Consortium ◽  
Incubation Temperature ◽  
Organic Phosphorus ◽  
High Sensitivity ◽  
Cell Number ◽  
Growth Potential ◽  
Utilization Efficiency ◽  
Available Phosphorus ◽  
Time Saving

Microbially available phosphorus (MAP) is the labile phosphorus that is readily assimilated by microorganisms, which is linearly correlated to bacterial re-growth in drinking water in some regions. The conventional MAP bioassay for drinking water was originally developed by Markku based on the growth potential of Pseudomonas fluorescens P17 (P17). However, the bioassay bears some demerits, such as time-consuming and labor-intensive enumeration. For convenience, an alternative method based on a similar principle was developed to assess the content of MAP in drinking water, in which natural microbial consortium was used as inoculum instead of pure culture P17, cell number was counted using flow cytometry (FCM), and cultivation at 30 °C was adopted. Natural microbial consortium is able to efficiently utilize organic phosphorus and exhibit high sensitivity since more cells are produced per μg P utilized. FCM is a rapid method to count all bacteria growing in drinking water. With incubation temperature increasing up to 30 °C, there is a shorter test period (64 h), excellent sensitivity and better utilization efficiency for organic phosphorus. The results show that the developed bioassay is sensitive, time-saving and easily operated.

Resonance Frequency Change in Microcantilever-Based Sensor due to Humidity Variation

2013 ◽  
Vol 737 ◽  
pp. 176-182 ◽  
Author(s):  
Ratno Nuryadi ◽  
Arko Djajadi ◽  
Reyhan Adiel ◽  
Lia Aprilia ◽  
Nuning Aisah
Keyword(s):  
Environmental Monitoring ◽  
Resonance Frequency ◽  
Rapid Detection ◽  
High Sensitivity ◽  
Spring Constant ◽  
Dynamic Mode ◽  
Frequency Change ◽  
The Many

Microcantilever-based sensors have attracted interest in the last decade because of their small size, rapid detection and high sensitivity. This sensor can be applied in the many fields, i.e. physics, chemistry, biology, biochemistry, medical, and environment. In this paper, we describe microcantilever-based sensor for environmental monitoring, especially for a humidity detection. This sensor was operated in dynamic mode where a change in mass or spring constant of the microcantilever provides the resonance frequency change. Here, a change of humidity is detected by the resonance frequency and the amplitude changes. It is found that the increase in the humidity causes the decreasing the resonance frequency but increasing the amplitude. This result opens up the possibility of the humidity detection using microcantilever-based sensor.

scholarly journals Simple and Portable Magnetic Immunoassay for Rapid Detection and Sensitive Quantification of Plant Viruses

2015 ◽  
Vol 81 (9) ◽  
pp. 3039-3048 ◽  
Author(s):  
Stefanie Rettcher ◽  
Felicitas Jungk ◽  
Christoph Kühn ◽  
Hans-Joachim Krause ◽  
Greta Nölke ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Plant Pathogens ◽  
High Sensitivity ◽  
Potato Virus X ◽  
Plant Viruses ◽  
Economic Losses ◽  
Virus Concentration ◽  
Content Type ◽  
Agricultural Industry ◽  
Sum Frequency

ABSTRACTPlant pathogens cause major economic losses in the agricultural industry because late detection delays the implementation of measures that can prevent their dissemination. Sensitive and robust procedures for the rapid detection of plant pathogens are therefore required to reduce yield losses and the use of expensive, environmentally damaging chemicals. Here we describe a simple and portable system for the rapid detection of viral pathogens in infected plants based on immunofiltration, subsequent magnetic detection, and the quantification of magnetically labeled virus particles.Grapevine fanleaf virus(GFLV) was chosen as a model pathogen. Monoclonal antibodies recognizing the GFLV capsid protein were immobilized onto immunofiltration columns, and the same antibodies were linked to magnetic nanoparticles. GFLV was quantified by immunofiltration with magnetic labeling in a double-antibody sandwich configuration. A magnetic frequency mixing technique, in which a two-frequency magnetic excitation field was used to induce a sum frequency signal in the resonant detection coil, corresponding to the virus concentration within the immunofiltration column, was used for high-sensitivity quantification. We were able to measure GFLV concentrations in the range of 6 ng/ml to 20 μg/ml in less than 30 min. The magnetic immunoassay could also be adapted to detect other plant viruses, includingPotato virus XandTobacco mosaic virus, with detection limits of 2 to 60 ng/ml.

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