Antitumor and antioxidant activities of purple potato ethanolic extract and its interaction with liposomes, albumin and plasmid DNA

2021 ◽  
Author(s):  
Paulina Anita Strugała ◽  
Anna Urbaniak ◽  
Patryk Kuryś ◽  
Aleksandra Włoch ◽  
Teresa Kral ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Antioxidative Activity ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Ethanolic Extract

The aim of study was to broadly determine the biological activities of purple potato ethanolic extract of the Blue Congo variety (BCE). The antioxidative activity of BCE was determined in...

Determination of Antioxidant Activities and Bioactive Components of Foeniculum vulgare Mill. (Samonsabar)

2019 ◽  
Vol 30 (3) ◽  
pp. 16-22
Keyword(s):  
Antioxidant Activities ◽  
Biological Activities ◽  
Scientific Information ◽  
Ethanolic Extract ◽  
The Body ◽  
World Health ◽  
Gas Chromatography Mass Spectrometry ◽  
Molecular Formula ◽  
Bioactive Components ◽  
Foeniculum Vulgare

World Health Organization (WHO) estimated that 80% of the population of developing countries use traditional medicines, mostly natural plant products, for their primary health care needs. In the past few decades, the medicinal value of plants has been assumed more important dimension owing largely to the discovery that extracts from plants contain not only primary metabolites but also a diverse array of secondary metabolites with antioxidant potential. Medicinal plants are potential sources of natural compounds with biological activities and therefore attract the attention of researchers worldwide. Antioxidants are vital substances which possess ability to protect the body from damage due to free radical-induced oxidative stress. The purpose of current study was to determine the antioxidant activities and bioactive components of Foeniculum vulgare (fennel) (Samonsabar) seeds by using UV Visible Spectrophotometer (UV-Vis) and Gas Chromatography-Mass Spectrometry (GC-MS). Aqueous extract of fennel seeds showed more antioxidant activity (IC50: 0.28 ug/ml) than ethanolic extract (IC50: 0.83 ug/ml) and comparable to standard antioxidant, ascorbic acid (IC50: 0.59 ug/ml). GC-MS analysis was fruitful in identification of compounds based on peak area, retention time, molecular formula, molecular weight, MS Fragmentions and pharmacological actions. Ten bioactive phytochemical compounds from aqueous extracts and 11 from ethanolic extract of fennel seeds were identified. These findings indicated that fennel seeds are potential to provide preventive properties against oxidative damage. These results will give scientific information for quality control of indigenous drug to herbal medicine users and local practitioners using fennel for different types of ailments

Management of Dermatophytoses by Foliose Lichen; Heterodermia diademata (Taylor) D.D. Awasthi

2017 ◽  
Vol 5 (2) ◽  
pp. 79-84
Author(s):  
Anand Pandey ◽  
◽  
Awadhesh Kumar ◽  
Anupam Dikshit ◽  
◽  
...  
Keyword(s):  
Fungicidal Activity ◽  
Trichophyton Rubrum ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Ethanolic Extract ◽  
Microsporum Gypseum ◽  
Superficial Infection ◽  
Recent Advancement ◽  
Secondary Substances ◽  
Foliose Lichen

Lichens are demarcated as an association of two distinct and dissimilar components; the mycobiont (a fungus) and the phycobionts (an alga) forming a composite organism. The recent advancement in science has paid much attention on the biological roles of lichen secondary substances; which are attributed with a lot of biological activities, such as antibacterial, antifungal, antiviral, anti-inflammatory, anti-tumor, and antioxidant activities. The current research aims to unravel the fungicidal activity of lichen Heterodermia diademata, ethanolic extract (HDE) against prevalent superficial infection causing dermatophytes viz., Trichophyton rubrum, T. mentagrophytes, Microsporum gypseum and M. canis. The HDE was found active against M. gypseum and T. mentagrophytes with MIC; 1.68 and 1.98 mg/ml and mg/ml respectively. The dermatophytes were also analyzed by molecular phylogeny of chs-1 gene for variability in MIC and prediction of susceptible pathogens.

scholarly journals Pharmacological Potential and Chemical Composition of Crocus sativus Leaf Extracts

Molecules ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 10
Author(s):  
Olha Mykhailenko ◽  
Vilma Petrikaite ◽  
Michal Korinek ◽  
Fang-Rong Chang ◽  
Mohamed El-Shazly ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Ethanolic Extract ◽  
Dry Weight ◽  
Crocus Sativus ◽  
Leaf Extracts ◽  
Hplc Fingerprint ◽  
Health Related ◽  
Pharmacological Potential

Crocus sativus L. (saffron) has been traditionally used as a food coloring or flavoring agent, but recent research has shown its potent pharmacological activity to tackle several health-related conditions. Crocus sp. leaves, and petals are the by-products of saffron production and are not usually used in the medicine or food industries. The present study was designed to determine the chemical composition of the water and ethanolic extracts of C. sativus leaves and test their cytotoxic activity against melanoma (IGR39) and triple-negative breast cancer (MDA-MB-231) cell lines by MTT assay. We also determined their anti-allergic, anti-inflammatory, and anti-viral activities. HPLC fingerprint analysis showed the presence of 16 compounds, including hydroxycinnamic acids, xanthones, flavonoids, and isoflavonoids, which could contribute to the extracts’ biological activities. For the first time, compounds such as tectoridin, iristectorigenin B, nigricin, and irigenin were identified in Crocus leaf extracts. The results showed that mangiferin (up to 2 mg/g dry weight) and isoorientin (8.5 mg/g dry weight) were the major active ingredients in the leaf extracts. The ethanolic extract reduced the viability of IGR39 and MDA-MB-231 cancer cells with EC50 = 410 ± 100 and 330 ± 40 µg/mL, respectively. It was more active than the aqueous extract. Kaempferol and quercetin were identified as the most active compounds. Our results showed that Crocus leaves contain secondary metabolites with potent cytotoxic and antioxidant activities.

scholarly journals PHYTOCHEMICAL SCREENING, ANTIOXIDANT AND ANTIMICROBIAL ASSESSMENT OF PLUCHEA INDICA (L.) LESS EXTRACT AS AN ACTIVE INGREDIENT IN NATURAL LOTION BAR

2021 ◽  
pp. 51-57
Author(s):  
SIRIKHWAN TINRAT
Keyword(s):  
Broad Spectrum ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Antimicrobial Activities ◽  
Dilution Method ◽  
Reducing Ability ◽  
Phytochemical Compounds ◽  
Antioxidant And Antimicrobial Activities

Objective: To screen phytochemical compounds and study antioxidant and antimicrobial activities of Pluchea indica (L.) Less (PI) leaves extracts for assessment the moisturizing lotion bar formulation containing it’s as a bioactive ingredient. Methods: PI leaves were extracted in different solvents. Crude extracts were screened phytochemical compounds and evaluated antioxidant and antimicrobial activities before formulating to antibacterial moisturizing lotion bar. Results: 95% ethanolic extract displayed high antioxidant activities. DPPH radical scavenging capacity with IC50 was 6.80±0.04 mg/ml (61.37±0.41%) and the ferric reducing ability power was 381.20±0.10 mg AAE/100 gDW. Additionally, 95% ethanolic extract served as a broad spectrum bacteriostatic agents against gram-positive and gram-negative species with an inhibition zone of 6.50±0.00-46.67±0.58 mm. It also was simultaneously as a broad spectrum bactericidal agents (MIC and MBC value of 25-200 mg/ml) by broth micro-dilution method. The screened phytochemical constituents (saponins, tannins, flavonoids, terpenoids, steroids, and alkaloids) in PI leaves extract may be responsible for high biological activities. Thus, 95% ethanolic extract was the most appropriate bioactive ingredient for formulated the product development. Conclusion: The developed moisturizing lotion bar with 0.0041% of 95% ethanolic extract was the most eligible formula that showed good appearance, odor, color, homogeneity, after feel and stability. This extract-containing lotion bar formulation achieved a high level of customer satisfaction by sensory evaluation and development of this specific formulation should be subject to further clinical investigation.

scholarly journals HPLC Analysis and Antioxidant Evaluation of Acteoside-Rich Osmanthus fragrans Extracts

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Sung-Ho Lee ◽  
Seong-Wook Seo ◽  
Seung-Yub Song ◽  
Deuk-Sil Oh ◽  
Jung-Hyun Shim ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Hot Water ◽  
Total Phenolic ◽  
Osmanthus Fragrans

A method for the separation and quantification of acteoside by reverse-phase high-performance liquid chromatography (HPLC) was developed and validated. Hot water and ethanolic extracts of Osmanthus fragrans leaves and flowers were analyzed for acteoside content. Excellent linearity was obtained, with an r2 higher than 0.999. The precision, specificity, and accuracy of our method were excellent, suggesting that it can be conveniently used for the quantification of acteoside in the crude extract of O. fragrans. The hot water and ethanol extracts were analyzed, and their biological activities were tested. The extraction yields, marker (acteoside) contents, and antioxidant activities of the leaf and flower extracts were analyzed. The antioxidant activity was confirmed by measuring the 2,2-diphenyl-2-picrylhydrazyl radical scavenging activity, reducing power, and total phenolic content. The acteoside content tended to be higher in the 100% ethanol extract of O. fragrans compared to those with the other extraction conditions tested. Overall, almost all extracts prepared with ethanolic solvents tended to produce better antioxidant activity than those prepared with hot water. These results suggest that the ethanolic extract of O. fragrans could serve as a potential antioxidant and anti-inflammatory pharmaceutical source, and our validated method would be useful for the quality control of O. fragrans extracts.

Biological Activities and Dermal Penetration of Liposome-Containing Coprinus atramentraris Extract

2019 ◽  
Vol 819 ◽  
pp. 124-129
Author(s):  
Parapat Sobharaksha ◽  
Ratana Indranupakorn ◽  
Sonmkamol Manchan
Keyword(s):  
Particle Size ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Skin Permeation ◽  
Ethanolic Extract ◽  
Entrapment Efficiency ◽  
Strong Relationship ◽  
Antioxidant Property ◽  
Total Phenolic

The total phenolic content and antioxidant activities of five edible mushrooms (Pleurotus djamor, Agaricus bisporus, Hericlum erinaceus, Coprinus atramentraris and P. ostreatus) were evaluated. The Coprinus atramentraris ethanolic extract (CE) showed the promising antioxidant property and had a strong relationship with phenolic compound content. Liposome-containing CE formulations, were prepared with various lipid composition. The liposome formulation, L1, which composed of phospholipids and cholesterol at ratio of 6:4 gave the highest entrapment efficiency (24.03%), small particle size (143.03 nm) and favorable particle size distribution (0.311) with small values of zeta potential (-30.2 mV). L1 showed good anti-elastase property (IC50 0.029) and no cytotoxicity effect to the human skin fibroblasts and melanoma cells even at the highest concentration of 1 mg/mL compared to the unformulated extract. The in vitro skin permeation studies, using human cadaver skin and modified Franz-diffusion cells, showed that the L1 was able to considerably increase the rate of permeation of phenolic compounds in L1 compared to the CE solution. These results reflect the use of L1 as active agents in cosmeceuticals.

scholarly journals Antimicrobial and antioxidant activity of essential oil and different plant extracts of Psidium cattleianum Sabine

2016 ◽  
Vol 76 (1) ◽  
pp. 101-108 ◽  
Author(s):  
M. C. Scur ◽  
F. G. S. Pinto ◽  
J. A. Pandini ◽  
W. F. Costa ◽  
C. W. Leite ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Essential Oil ◽  
Plant Extracts ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Ethanolic Extract ◽  
Phytochemical Screening ◽  
Psidium Cattleianum ◽  
Ethanolic Extracts

Abstract The goals of the study were to determinethe antimicrobial and antioxidant activities of essential oil and plant extracts aqueous and ethanolic of Psidium cattleianum Sabine; the chemical composition of the essential oil of P. cattleianum; and the phytochemical screening of aqueous and ethanolic extracts of the same plant. Regarding the antimicrobial activity, the ethanolic extract exhibited moderate antimicrobial activity with respect to bacteria K. pneumoniae and S. epidermidis, whereas, regarding other microorganisms, it showed activity considered weak. The aqueous extract and the essential oil showed activity considered weak, although they inhibited the growth of microorganisms. About the antioxidant potential, the ethanolic and aqueous extracts exhibited a scavenging index exceeding 90%, while the essential oil didn´t show significant antioxidant activity. Regarding the phytochemical composition, the largest class of volatile compounds identified in the essential oil of P. cattleianum included the following terpenic hydrocarbons: α-copaene (22%); eucalyptol (15%), δ-cadinene (9.63%) and α-selinene (6.5%). The phytochemical screening of extracts showed the presence of tannins, flavonoids, and triterpenoids for aqueous and ethanolic extracts. The extracts and essential oils inhibit the growth of microrganisms and plant extracts showed significant antioxidant activity. Also, the phytochemical characterization of the essential oil showed the presence of compounds interest commercial, as well as extracts showed the presence of important classes and compounds with biological activities.

scholarly journals Novel Antiviral and Antibacterial Activities of Hibiscus schizopetalus

Antibiotics ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 756
Author(s):  
Riham A. El-Shiekh ◽  
Usama Ramadan Abdelmohsen ◽  
Hossam M. Ashour ◽  
Rehab M. Ashour
Keyword(s):  
Antioxidant Activities ◽  
Biological Activities ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Antibacterial Activities ◽  
Resistant Bacteria ◽  
Type I ◽  
Chemical Profiles ◽  
Simplex Virus

Hibiscus schizopetalus (Dyer) Hook.f. (Malvaceae) is an ornamental plant. The aim was to investigate its antimicrobial and antioxidant activities. In vitro antiviral, antibacterial, and antioxidant activities of the 70% ethanolic extract (Et-E) of the aerial parts of the plant were determined. The Dichloromethane Fraction (DCM-F) and the n-Butanol Fraction (Bu-F) were assessed using Liquid chromatography–mass spectrometry (LC-MS). The DCM-F showed higher antiviral activities against Coxsackie B4 (CoxB4) viruses (IC50 = 64.13 µg/mL) and adenoviruses (IC50 = 54.88 µg/mL) than acyclovir (IC50 = 72.79 µg/mL for CoxB4 viruses; IC50 = 91.92 µg/mL for adenoviruses). The DCM-F showed higher anti-helicobacter pylori activity (MIC = 3.9 µg/mL) than clarithromycin (MIC = 1.95 µg/mL). The DCM-F inhibited Herpes Simplex Virus (HSV) Type I (IC50 = 29.85 µg/mL) and HSV Type II (IC50 = 74.17 µg/mL). The Bu-F showed higher anti-mycobacterial activity (MIC = 7.81 µg/mL) than isoniazid (MIC = 0.24 µg/mL) and higher antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA)(MIC = 7.81 µg/mL) than vancomycin (MIC = 3.9 µg/mL). Antioxidant assays included total antioxidant capacity (TAC), 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), 2,2-diphenyl-1-picryl-hydrazyl (DPPH), and iron reducing power. The Bu-F showed the highest antioxidant activity. Chemical profiles were analyzed using HPLC-HR–ESI–MS to identify the metabolites responsible for these biological activities. We identified more than 60 metabolites that belong to anthocyanins, flavonoids, phenolics, terpenes, sterols, and fatty acids. In conclusion, Hibiscusschizopetalus is endowed with metabolites that could be used against viruses and antibiotic-resistant bacteria. They can also be potent antioxidants.

scholarly journals LC-HRMS Profiling and Antidiabetic, Anticholinergic, and Antioxidant Activities of Aerial Parts of Kınkor (Ferulago stellata)

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2469
Author(s):  
Hatice Kızıltaş ◽  
Zeynebe Bingol ◽  
Ahmet Ceyhan Gören ◽  
Leyla Polat Kose ◽  
Lokman Durmaz ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
High Resolution Mass Spectrometry ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Water Extract ◽  
Ethanolic Extract ◽  
Metabolic Enzyme ◽  
Scavenging Activity ◽  
Ferrous Ions ◽  
Water Extracts

Kınkor (Ferulago stellata) is Turkish medicinal plant species and used in folk medicine against some diseases. As far as we know, the data are not available on the biological activities and chemical composition of this medicinal plant. In this study, the phytochemical composition; some metabolic enzyme inhibition; and antidiabetic, anticholinergic, and antioxidant activities of this plant were assessed. In order to evaluate the antioxidant activity of evaporated ethanolic extract (EEFS) and lyophilized water extract (WEFS) of kınkor (Ferulago stellata), some putative antioxidant methods such as DPPH· scavenging activity, ABTS•+ scavenging activity, ferric ions (Fe3+) reduction method, cupric ions (Cu2+) reducing capacity, and ferrous ions (Fe2+)-binding activities were separately performed. Furthermore, ascorbic acid, BHT, and α-tocopherol were used as the standard compounds. Additionally, the main phenolic compounds that are responsible for antioxidant abilities of ethanol and water extracts of kınkor (Ferulago stellata) were determined by liquid chromatography-high-resolution mass spectrometry (LC-HRMS). Ethanol and water extracts of kınkor (Ferulago stellata) demonstrated effective antioxidant abilities when compared to standards. Moreover, ethanol extract of kınkor (Ferulago stellata) demonstrated IC50 values of 1.772 μg/mL against acetylcholinesterase (AChE), 33.56 ± 2.96 μg/mL against α-glycosidase, and 0.639 μg/mL against α-amylase enzyme respectively.

Improvement of cytoprotective and antioxidant activity of Rosa canina L. and Salix alba L. by controlled differential sieving process against H2O2-induced oxidative stress in mouse primary splenocytes

2017 ◽  
Vol 87 (3-4) ◽  
pp. 191-200 ◽  
Author(s):  
Nidhal Soualeh ◽  
Aliçia Stiévenard ◽  
Elie Baudelaire ◽  
Rachid Soulimani ◽  
Jaouad Bouayed
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Good Alternative ◽  
Activity Levels ◽  
Salix Alba ◽  
Rosa Canina ◽  
Free Process ◽  
Plant Powders ◽  
Sieving Process

Abstract. In this study, cytoprotective and antioxidant activities of Rosa canina (RC) and Salix alba (SA), medicinal plants, were studied on mouse primary splenocytes by comparing Controlled Differential Sieving process (CDSp), which is a novel green solvent-free process, versus a conventional technique, employing hydroethanolic extraction (HEE). Thus, preventive antioxidant activity of three plant powders of homogeneous particle sizes, 50–100 µm, 100–180 µm and 180–315 µm, dissolved directly in the cellular buffer, were compared to those of hydroethanolic (HE) extract, at 2 concentrations (250 and 500 µg/mL) in H2O2-treated spleen cells. Overall, compared to HE extract, the superfine powders, i. e., fractions < 180 µm, at the lowest concentration, resulted in greater reactive oxygen species (ROS) elimination, increased glutathione peroxidase (GPx) activity and lower malondialdehyde (MDA) production. Better antioxidant and preventive effects in pre-treated cells were found with the superfine powders for SA (i. e., 50–100 µm and 100–180 µm, both p < 0.001), and with the intermediate powder for RC (i. e., 100–180 µm, p < 0.05) versus HE extract. The activity levels of catalase (CAT) and superoxide dismutase (SOD) in pretreated splenocytes exposed to H2O2, albeit reduced, were near to those in unexposed cells, suggesting that pretreatment with the fine powders has relatively restored the normal levels of antioxidant-related enzymes. These findings supported that CDSp improved the biological activities of plants, avoiding the use of organic solvents and thus it could be a good alternative to conventional extraction techniques.

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