scholarly journals Lipids associated with bovine kidney glomerular basement membranes

1976 ◽  
Vol 155 (3) ◽  
pp. 535-541 ◽  
Author(s):  
G Kibel ◽  
A Heilhecker ◽  
F Von Bruchhausen
Keyword(s):  
Fatty Acids ◽  
Basement Membrane ◽  
Lipid Content ◽  
Glomerular Basement Membrane ◽  
Membrane Lipids ◽  
Neutral Lipids ◽  
Methyl Esters ◽  
Specific Radioactivity ◽  
Basement Membranes ◽  
Total Lipid Extract

1. After incubation of bovine glomeruli with D-[U-14C]glucose, about 21% of the total radioactivity is found in lipid extracts of glomerular basement membranes. 2. The concentration of lipids in glomerular basement membranes (4.3% of dry wt.) is lower than in the residual glomerular particles (10.8% of dry wt.). The concentrations of neutral lipids (13.9%), phospholipids (46.7%) and cholesterol (37.9%) in the total lipid extract of the glomerular basement membranes, however, differ from those in the residual glomerular particles (15.6, 54.0 and 30.9% respectively). Though residual glomerular particles show a higher lipid content, the radioactivity in this fraction only amounts to 38% of that found in the glomerular basement membranes. 3. The specific radioactivity of total glomerular basement-membrane lipids (12 600 d.p.m./mg) is about 4 times as high as that of the glomerular basement membranes. The specific radioactivities of the individual lipid components, however, differ. The highest values are found for phosphatidylcholine and triacylglycerols. The largest proportion of the radioactivity is found in the glycerol of the glycerides. The radioactivity in the fatty acids is much less and does not differ significantly in the various classes of lipids. 4. G.1.c. of methyl esters of the fatty acids does not reveal a clear difference between the fatty acid compositions of glomerular basement membranes and residual glomerular particles. 5. Treatment of glomerular basement-membrane preparations with ultrasound, the generally used procedure for glomerular basement-membrane preparations, drastically decreases the lipid content of glomerular basement membranes. 6. It is concluded that lipids are associated with the basement membranes. Further, the comparatively high radioactive labelling suggests that glomerular basement-membrane lipids may be an interesting class of substances for further pathological studies.

scholarly journals SARS-CoV-2 infection and recurrence of anti-glomerular basement disease: a case report

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Alexander Winkler ◽  
Emanuel Zitt ◽  
Hannelore Sprenger-Mähr ◽  
Afschin Soleiman ◽  
Manfred Cejna ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Plasma Cells ◽  
Rapidly Progressive Glomerulonephritis ◽  
Kidney Injury ◽  
Pulmonary Involvement ◽  
Pulmonary Haemorrhage ◽  
Basement Membranes ◽  
High Avidity ◽  
History Of

Abstract Background Anti-glomerular basement membrane disease (GBM) disease is a rare autoimmune disease causing rapidly progressive glomerulonephritis and pulmonary haemorrhage. Recently, an association between COVID-19 and anti-glomerular basement membrane (anti-GBM) disease has been proposed. We report on a patient with recurrence of anti-GBM disease after SARS-CoV-2 infection. Case presentation The 31-year-old woman had a past medical history of anti-GBM disease, first diagnosed 11 years ago, and a first relapse 5 years ago. She was admitted with severe dyspnoea, haemoptysis, pulmonary infiltrates and acute on chronic kidney injury. A SARS-CoV-2 PCR was positive with a high cycle threshold. Anti-GBM autoantibodies were undetectable. A kidney biopsy revealed necrotising crescentic glomerulonephritis with linear deposits of IgG, IgM and C3 along the glomerular basement membrane, confirming a recurrence of anti-GBM disease. She was treated with steroids, plasma exchange and two doses of rituximab. Pulmonary disease resolved, but the patient remained dialysis-dependent. We propose that pulmonary involvement of COVID-19 caused exposure of alveolar basement membranes leading to the production of high avidity autoantibodies by long-lived plasma cells, resulting in severe pulmonary renal syndrome. Conclusion Our case supports the assumption of a possible association between COVID-19 and anti-GBM disease.

scholarly journals Fatty Acids Profiles of Stipe and Blade from the Norwegian Brown Macroalga Laminaria hyperborea, Using Off-Line SPE and GC-MS

2016 ◽  
Author(s):  
Lena Oksdøl Foseid ◽  
Hanne Devle ◽  
Yngve Stenstrøm ◽  
Carl Fredrik Naess-Andresen ◽  
Dag Ekeberg
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Neutral Lipids ◽  
Methyl Esters ◽  
Lipid Fraction ◽  
Environmental Benefits ◽  
Laminaria Hyperborea ◽  
Alpha Linolenic Acid ◽  
Lipid Fractions

A thorough analysis and comparison of the fatty acid profiles of stipe and blade from Laminaria hyperborea, a kelp species found in the northern Atlantic, is presented. Lipids were extracted and fractionated into neutral lipids, free fatty acids and polar lipids, then derivatized to fatty acid methyl esters prior to GC-MS analysis. A total of 42 fatty acids were identified and quantified, including the n-3 fatty acids α-linolenic acid, stearidonic acid and eicosapentaenoic acid. An n-6/n-3 ratio of 0.8:1 was found in blade and 3.5:1 in stipe, respectively. The ratios vary between the lipid fractions within stipe and blade, with the lowest ratio in the polar lipid fraction of blade. The fatty acid amounts are higher in blade than in stipe, and the highest amounts of n-3 fatty acids are found within the neutral lipid fractions. The amounts of polyunsaturated fatty acids are 3.4 times higher in blade than stipe. This study highlights the compositional differences between the lipid fractions of stipe and blade from L. hyperborea. The amount of polyunsaturated fatty acids, compared to saturated- and monounsaturated fatty acids, as well as the n-6/n-3-ratio, is known to influence human health. In the pharmaceutical, food, and feed industries this can be of importance for production and sale of different health products. Additionally, lipids are today among the unused by products of alginate production, exploiting this material for commercial interest should give both economical and environmental benefits.

In vivo biosynthesis and turnover of glomerular basement membrane in diabetic rats

1982 ◽  
Vol 242 (4) ◽  
pp. F385-F389
Author(s):  
M. P. Cohen ◽  
M. L. Surma ◽  
V. Y. Wu
Keyword(s):  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Diabetic Rats ◽  
Basement Membranes ◽  
Membrane Turnover ◽  
Proline Incorporation ◽  
Basement Membrane Collagen ◽  
Osmotic Lysis ◽  
Specific Activities

Glomerular basement membrane (GBM) was labeled in vivo by the injection of tracer amounts of tritiated proline into normal and streptozotocin-diabetic rats. Basement membrane biosynthesis and turnover were determined from the specific activities of proline and hydroxyproline in samples purified following osmotic lysis of glomeruli isolated 4 h to 12 days after injection. Peak radiolabeling of normal and diabetic GBM occurred within 24-48 h and 48-72 h, respectively, and, when corrected for differences in the serum proline specific activities, [3H]proline incorporation was greater in diabetic than in normal samples. In contrast to the subsequent time-dependent progressive decline in radiolabeling in basement membranes from normal animals, specific activities of proline and hydroxyproline in diabetic glomerular basement membrane did not change significantly over the same period of observation. Renal cortical mass and glomerular basement membrane collagen content were preserved in diabetic animals despite loss of body weight. The findings are compatible with prolongation of glomerular basement membrane turnover in experimental diabetes, and suggest that diminished degradation contributes to the accumulation of glomerular basement membrane that is characteristic of chronic diabetes.

scholarly journals Anionic charge concentration of rat kidney glomeruli and glomerular basement membrane

1993 ◽  
Vol 289 (3) ◽  
pp. 647-652 ◽  
Author(s):  
W D Comper ◽  
A S N Lee ◽  
M Tay ◽  
Y Adal
Keyword(s):  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Electrostatic Interactions ◽  
Enzyme Inhibitors ◽  
Rat Kidney ◽  
Basement Membranes ◽  
Sulphate Content ◽  
Anionic Charge ◽  
Charge Concentration ◽  
A Charge

Estimates of levels of glomerular and glomerular-basement-membrane anion charge should serve as useful quantitative markers for the integrity of the tissues in health and disease. We have developed a simple, rapid, technique to measure this charge through the use of ion exchange with radioisotopes 22Na+ and 36Cl- at low ionic strengths in phosphate buffer. When this technique is used, normal glomeruli isolated from rat have a measured net anion charge concentration of 17.4 +/- 3.7 p-equiv. per glomerulus (n = 20). Perfused rat kidneys that lose approximately half of their glomerular heparan [35S]sulphate content (owing to oxygen-radical damage) exhibited a lower anion charge, of 7.5 +/- 1.6 p-equiv. per glomerulus (n = 5). Glomerular basement membranes prepared from rat glomeruli by a sonication-centrifugation procedure in the presence of enzyme inhibitors had a charge concentration of 6.3 +/- 0.7 mu-equiv./g wet wt. of tissue (n = 4), whereas membranes prepared by sonication, centrifugation, DNAse and detergent treatment had a charge concentration of 7.1 +/- 1.6 mu-equiv./g wet wt. (n = 4). Isotope-dilution experiments with 3H2O on these detergent-prepared glomerular basement membranes demonstrated that they had a water content of approx. 93%, which would then give a net anion charge concentration of 7.6 +/- 1.7 m-equiv./l (n = 4). These values are in good agreement with those obtained by others using titration techniques [Bray and Robinson (1984) Kidney Int. 25, 527-533]. The relatively low magnitude of glomerular anion charge in normal kidneys is consistent with other recent findings that glomerular anion charge is too low to affect the glomerular transport of charged molecules in a direct, passive, biophysical manner through electrostatic interactions.

scholarly journals FATTY ACIDS COMPOSITION OF TOCTE (JUGLANS NEOTROPICA DIELS) WALNUT FROM ECUADOR

2018 ◽  
Vol 11 (2) ◽  
pp. 395 ◽  
Author(s):  
Vilcacundo E ◽  
Alvarez M ◽  
Silva M ◽  
Carpio C ◽  
Morales D ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Oleic Acid ◽  
Linoleic Acid ◽  
Stearic Acid ◽  
Palmitic Acid ◽  
Linolenic Acid ◽  
Lipid Content ◽  
Methyl Esters ◽  
Total Lipid Content ◽  
Fatty Acids Composition

 Objective: The aim of this study was to determine the fatty acids composition in a tocte seeds oil (Juglans neotropica Diels) sample cultivated in Ecuador.Methods: Tocte oil was obtained from tocte seeds using the cold pressing method. Fatty acids analysis was carried out using the gas chromatography method with a mass selective detector (GC/MSD) and using the database Library NIST14.L to identify the compounds.Results: Methyl esters fatty acids were identified from tocte (J. neotropica Diels) walnut using the GC–MS analytical method. The total lipid content of tocte walnuts seeds of plants cultivated in Ecuador was of 49.01% of the total lipid content on fresh weight. Fatty acids were analyzed as methyl esters on a capillary column DB-WAX 122-7062 with a good separation of palmitic acid, stearic acid, oleic acid, linoleic acid, and linolenic acid. The structure of methyl esters fatty acids was determined using the GC–MS. Tocte walnut presents 5.05% of palmitic acid, 2.26% of stearic acid, 19.50% of oleic acid, 65.81% of linoleic acid, and 2.79% linolenic acid of the total content of fatty acids in tocte oil. Fatty acids content reported in this study were similar to the data reported for other walnuts seeds.Conclusions: Tocte seeds are a good source of monounsaturated and polyunsaturated fatty acids. Tocte oil content oleic acid and with a good content of ɷ6 α-linoleic and ɷ3 α-linolenic. Tocte walnut can help reduce risk cardiovascular diseases in Ecuador for their good composition of fatty acids.

Etude de la constitution lipidique des membranes de bactéries lactiques utilisées en vinification

OENO One ◽  
1988 ◽  
Vol 22 (1) ◽  
pp. 25
Author(s):  
Catherine Desens ◽  
Aline Lonvaud-Funel
Keyword(s):  
Cell Cycle ◽  
Fatty Acids ◽  
Plasma Membrane ◽  
Culture Medium ◽  
Membrane Lipids ◽  
Neutral Lipids ◽  
Variable Response ◽  
Couche Mince

<p style="text-align: justify;">Les lipides extraits des membranes de bactéries lactiques du vin sont séparés par chromatographie sur couche mince en phospholipides, glycolipides et lipides neutres. Le pourcentage de répartition de chaque classe varie, non seulement en fonction des souches, mais aussi selon leurs conditions de culture.</p><p style="text-align: justify;">+++</p><p style="text-align: justify;">Lactic and bacteria of wines are inhibited in fermenting must. Éthanol, fatty acids and other unknow yeast metabolites are responsible for the loss of viability and malolactic activity. Prior results suggest that they induce an alteration of the plasma membrane. Owing to their nature, we assume the lipid constituants to be the target of the inhibitors. So, we have undertaken the study of the membrane lipids of two strains, <em>L. plantarum</em> and <em>L. oenos</em>.</p><p style="text-align: justify;">After extraction, the samples are analysed by HPTLC. Comparing the strains, the patterns reveal small differences in the distribution of phospholipids, glycolipids and neutral lipids.</p><p style="text-align: justify;">Some culture factors were investigated for their influence. Within the cell cycle, the distribution of the various classes changes according to the period of incubation. If ethanol is added in the culture medium, neutral lipids decrease by 25 to 50 per cent and glycolipids increase by 30 per cent depending on the age of the culture. Phospholipids remain quite constant.</p><p style="text-align: justify;">Further researchs are in progress to test the influence of others factors. These results might explain the loss of viability of the bacteria varies when they are added to wine, as well as the variable response to yeast antagonism according to the strains.</p>

scholarly journals Identification and Quantification of Fatty Acids in T. viridissima, C. biguttulus, and C. brunneus by GC-MS

2018 ◽  
Vol 2018 ◽  
pp. 1-8
Author(s):  
Alexander M. Wathne ◽  
Hanne Devle ◽  
Carl Fredrik Naess-Andresen ◽  
Dag Ekeberg
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Solid Phase Extraction ◽  
Free Fatty Acids ◽  
Dry Matter ◽  
Solid Phase ◽  
Neutral Lipids ◽  
Methyl Esters ◽  
Phase Extraction ◽  
Chorthippus Biguttulus

Fatty acid (FA) profiles of the species Tettigonia viridissima, Chorthippus biguttulus, and Chorthippus brunneus were determined and quantitated. Extracted lipids were derivatized into FA methyl esters (FAMEs) prior to analysis by GC-MS. A total of 37 different FAs were identified in T. viridissima, yielding a total FA content of 10.4 g/100 g of dry matter. The contents of saturated FAs, monounsaturated FAs, and polyunsaturated FAs were 31.1, 35.9, and 33.0%, respectively. Lipids from T. viridissima were also fractioned into neutral lipids, free fatty acids, and polar lipids by offline solid phase extraction. For C. brunneus and C. biguttulus, 33 FAs were identified, yielding a total FA content of 6.14 g/100 g of dry matter. SFAs, MUFAs, and PUFAs, respectively, constituted 32.7, 25.1, and 42.1% of the total FA content. The contents of MUFAs, PUFAs, n-3 FAs, and n-6 FAs of each species, and the n-6/n-3 ratio, were subsequently discussed.

scholarly journals LIPIDS OF CARDIOMYOCYTES MEMBRANES STRUCTURES IN RATS AT HYPOBIOSIS

2016 ◽  
Vol 1 ◽  
pp. 3-8
Author(s):  
Svitlana Khyzhnyak ◽  
Svitlana Midyk ◽  
Serhii Sysoliatin ◽  
Olena Laposha
Keyword(s):  
Fatty Acids ◽  
Unsaturated Fatty Acids ◽  
Membrane Lipids ◽  
Neutral Lipids ◽  
Fatty Acid Content ◽  
Biologically Active ◽  
Membrane Phospholipids ◽  
Internal Membrane ◽  
Regulatory Systems ◽  
Artificial Hypobiosis

There was studied an influence of artificial hypobiosis in the conditions of hypoxia-hypercarnia in rats on the quantity of neutral lipids, phospholipids and its fatty acids in membranes structures of cardiomyocytes (microsomes and internal membranes of mitochondria). The received results – the content of lipids, individual phospholipids, cholesterol testify to the modification of lipid component of cardiomyocytes mitochondria internal membrane (less the microsomal fraction) that characterizes modulation of cellular membranes structural-functional state. There was noticed the possibility of attraction of membrane phospholipids (sphingomyelin, phosphatidylserine and phosphatidylinositol) to the signal ways activation at hypobiosis. There was revealed redistribution of fatty acids of mitochondria internal membrane at hypobiosis that leads to increase of the level of unsaturated fatty acids. There was noticed the possibility of participation of monoenic unsaturated fatty acids in protection of cellular structures from oxidizing stress and increase of the content of arachidonic and docosahexaenoic acids – precursors of biologically active substances – can be connected with its attraction to regulatory systems at hypobiosis. There is presupposed that the state of artificial hypobiosis is characterized with stress-reaction that leads to optimal reconstruction of lipid and fatty acid content of membrane lipids directed on support of cardiomyocytes functional activity.

The effect of long term storage on the lipid reserves and fatty acid composition of cysts and hatched juveniles of Globodera rostochiensis and G. pallida

1998 ◽  
Vol 72 (2) ◽  
pp. 133-141 ◽  
Author(s):  
R.A. Holz ◽  
D.J. Wright ◽  
R.N. Perry
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Neutral Lipid ◽  
Lipid Content ◽  
Neutral Lipids ◽  
Lipid Fraction ◽  
Neutral Lipid Fraction ◽  
Fatty Acid Profiles ◽  
Acidic Phospholipids

AbstractThe lipid composition of three batches of single generation cysts of Globodera rostochiensis, stored dry at 4°C for 1,7 and 13 years, comprised 81%, 74% and 53% neutral lipids, 14%, 18% and 27% non-acidic phospholipids and 5%, 8% and 20% free fatty acids, respectively. Lipids in eggs from two batches of G. pallida cysts, stored for 3 and 7 years, comprised 80% and 67% neutral lipids, 15% and 23% non-acidic phospholipids and 5% and 10% free fatty acids, respectively. All batches contained the same fatty acids which were dominated by C18:l, C20:l and C20:4. The fatty acid profiles of hatched J2 of G. rostochiensis from two batches, stored for 1 and 9 years, differed only in their free fatty acid fractions. Thus, while it is not possible to determine the age of cysts by their fatty acid profile, it may be possible to use the relative amounts of the main lipid classes as an indicator of age. Four batches of hatched J2 of G. pallida were investigated, with sample A hatched during the second week in potato root diffusate, B during week 3, C during week 4 and D during weeks 5 and 6 and stored for 3.5 days (on average) after hatching. Total lipid content was 27.2%, 31.5%, 18.5% and 6.3% of the dry weight for A, B, C and D, respectively. In the neutral lipid fraction of D an increase in C18:l and to a lesser extent C18:2 was observed. In the free fatty acid fraction of sample D, the percentages of C18:l, C18:2 and C18:3 were greater but the percentages of C20:3 and C20:4 were smaller compared with sample C. Fresh early hatched J2 of G. rostochiensis were compared with later hatched and stored (for 13 days on average) individuals for their lipid content and fatty acid composition. The lipid content was 26.1% and 11.4% in fresh and stored J2, respectively. Total lipid consisted of 77% and 70% neutral lipid, 18% and 26% non-acidic phospholipid and 6% and 4% free fatty acid in fresh and stored J2, respectively. In the neutral lipid fraction of stored J2 C18:l, C16:0 and C18:0 increased, whereas C20:4, C20:l and C20:3 decreased. Therefore, both neutral lipid and free fatty acid fractions showed changes in their fatty acid profiles after long delayed hatching and/or storage in both PCN species.

Évolution de la teneur en lipides de Leptosphaeria typhae en fonction de sa croissance et de sa reproduction sexuée : activité de synthèse et étude des lipides neutres et polaires

1985 ◽  
Vol 63 (12) ◽  
pp. 2460-2467
Author(s):  
Gérard Vidal
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acids ◽  
Lipid Content ◽  
Neutral Lipids ◽  
Fatty Acid Synthetase ◽  
Polar Lipids ◽  
Synthetase Activity ◽  
Lipid Turnover ◽  
Fatty Acid Synthetase Activity

When perithecia appear (on the 7th day of growth), the mycelial lipid content falls and the degree of fat insaturation increases; fatty acid synthetase activity greatly increases. Lipid turnover is therefore accelerated. Among neutral lipids, triglycerides constitute the largest pool and their proportion increases when perithecia appear. Free fatty acids also increase, confirming the lipid turnover's acceleration. Polar lipids especially disappear; the phosphatidylcholine/phosphatidylethanolamine ratio increases when the fungus ascosporulates.

Sign in / Sign up

Export Citation Format

Share Document