Etude de la constitution lipidique des membranes de bactéries lactiques utilisées en vinification

OENO One ◽  
1988 ◽  
Vol 22 (1) ◽  
pp. 25
Author(s):  
Catherine Desens ◽  
Aline Lonvaud-Funel
Keyword(s):  
Cell Cycle ◽  
Fatty Acids ◽  
Plasma Membrane ◽  
Culture Medium ◽  
Membrane Lipids ◽  
Neutral Lipids ◽  
Variable Response ◽  
Couche Mince

<p style="text-align: justify;">Les lipides extraits des membranes de bactéries lactiques du vin sont séparés par chromatographie sur couche mince en phospholipides, glycolipides et lipides neutres. Le pourcentage de répartition de chaque classe varie, non seulement en fonction des souches, mais aussi selon leurs conditions de culture.</p><p style="text-align: justify;">+++</p><p style="text-align: justify;">Lactic and bacteria of wines are inhibited in fermenting must. Éthanol, fatty acids and other unknow yeast metabolites are responsible for the loss of viability and malolactic activity. Prior results suggest that they induce an alteration of the plasma membrane. Owing to their nature, we assume the lipid constituants to be the target of the inhibitors. So, we have undertaken the study of the membrane lipids of two strains, <em>L. plantarum</em> and <em>L. oenos</em>.</p><p style="text-align: justify;">After extraction, the samples are analysed by HPTLC. Comparing the strains, the patterns reveal small differences in the distribution of phospholipids, glycolipids and neutral lipids.</p><p style="text-align: justify;">Some culture factors were investigated for their influence. Within the cell cycle, the distribution of the various classes changes according to the period of incubation. If ethanol is added in the culture medium, neutral lipids decrease by 25 to 50 per cent and glycolipids increase by 30 per cent depending on the age of the culture. Phospholipids remain quite constant.</p><p style="text-align: justify;">Further researchs are in progress to test the influence of others factors. These results might explain the loss of viability of the bacteria varies when they are added to wine, as well as the variable response to yeast antagonism according to the strains.</p>

scholarly journals LIPIDS OF CARDIOMYOCYTES MEMBRANES STRUCTURES IN RATS AT HYPOBIOSIS

2016 ◽  
Vol 1 ◽  
pp. 3-8
Author(s):  
Svitlana Khyzhnyak ◽  
Svitlana Midyk ◽  
Serhii Sysoliatin ◽  
Olena Laposha
Keyword(s):  
Fatty Acids ◽  
Unsaturated Fatty Acids ◽  
Membrane Lipids ◽  
Neutral Lipids ◽  
Fatty Acid Content ◽  
Biologically Active ◽  
Membrane Phospholipids ◽  
Internal Membrane ◽  
Regulatory Systems ◽  
Artificial Hypobiosis

There was studied an influence of artificial hypobiosis in the conditions of hypoxia-hypercarnia in rats on the quantity of neutral lipids, phospholipids and its fatty acids in membranes structures of cardiomyocytes (microsomes and internal membranes of mitochondria). The received results – the content of lipids, individual phospholipids, cholesterol testify to the modification of lipid component of cardiomyocytes mitochondria internal membrane (less the microsomal fraction) that characterizes modulation of cellular membranes structural-functional state. There was noticed the possibility of attraction of membrane phospholipids (sphingomyelin, phosphatidylserine and phosphatidylinositol) to the signal ways activation at hypobiosis. There was revealed redistribution of fatty acids of mitochondria internal membrane at hypobiosis that leads to increase of the level of unsaturated fatty acids. There was noticed the possibility of participation of monoenic unsaturated fatty acids in protection of cellular structures from oxidizing stress and increase of the content of arachidonic and docosahexaenoic acids – precursors of biologically active substances – can be connected with its attraction to regulatory systems at hypobiosis. There is presupposed that the state of artificial hypobiosis is characterized with stress-reaction that leads to optimal reconstruction of lipid and fatty acid content of membrane lipids directed on support of cardiomyocytes functional activity.

scholarly journals Lipids associated with bovine kidney glomerular basement membranes

1976 ◽  
Vol 155 (3) ◽  
pp. 535-541 ◽  
Author(s):  
G Kibel ◽  
A Heilhecker ◽  
F Von Bruchhausen
Keyword(s):  
Fatty Acids ◽  
Basement Membrane ◽  
Lipid Content ◽  
Glomerular Basement Membrane ◽  
Membrane Lipids ◽  
Neutral Lipids ◽  
Methyl Esters ◽  
Specific Radioactivity ◽  
Basement Membranes ◽  
Total Lipid Extract

1. After incubation of bovine glomeruli with D-[U-14C]glucose, about 21% of the total radioactivity is found in lipid extracts of glomerular basement membranes. 2. The concentration of lipids in glomerular basement membranes (4.3% of dry wt.) is lower than in the residual glomerular particles (10.8% of dry wt.). The concentrations of neutral lipids (13.9%), phospholipids (46.7%) and cholesterol (37.9%) in the total lipid extract of the glomerular basement membranes, however, differ from those in the residual glomerular particles (15.6, 54.0 and 30.9% respectively). Though residual glomerular particles show a higher lipid content, the radioactivity in this fraction only amounts to 38% of that found in the glomerular basement membranes. 3. The specific radioactivity of total glomerular basement-membrane lipids (12 600 d.p.m./mg) is about 4 times as high as that of the glomerular basement membranes. The specific radioactivities of the individual lipid components, however, differ. The highest values are found for phosphatidylcholine and triacylglycerols. The largest proportion of the radioactivity is found in the glycerol of the glycerides. The radioactivity in the fatty acids is much less and does not differ significantly in the various classes of lipids. 4. G.1.c. of methyl esters of the fatty acids does not reveal a clear difference between the fatty acid compositions of glomerular basement membranes and residual glomerular particles. 5. Treatment of glomerular basement-membrane preparations with ultrasound, the generally used procedure for glomerular basement-membrane preparations, drastically decreases the lipid content of glomerular basement membranes. 6. It is concluded that lipids are associated with the basement membranes. Further, the comparatively high radioactive labelling suggests that glomerular basement-membrane lipids may be an interesting class of substances for further pathological studies.

The lipid composition of plasma membrane from goat mammary gland

1988 ◽  
Vol 66 (12) ◽  
pp. 1355-1359 ◽  
Author(s):  
Arun Sharma ◽  
Rajvir Dahiya
Keyword(s):  
Fatty Acids ◽  
Plasma Membrane ◽  
Liquid Chromatography ◽  
Mammary Gland ◽  
Thin Layer ◽  
Lipid Composition ◽  
Cholesterol Ester ◽  
Plasma Membranes ◽  
Neutral Lipids ◽  
Gas Liquid Chromatography

Experiments were conducted to examine and characterize the lipid composition of the plasma membrane from the lactating goat mammary gland. The plasma membranes were purified by discontinuous sucrose density centrifugation. Lipids were extracted from these membranes and analyzed by thin-layer and gas–liquid chromatography. The results of these studies demonstrate that (i) the principal phospholipids of mammary-gland plasma membranes are phosphatidylcholine, phosphatidylethanolamine, and sphingomyelin; (ii) the principal neutral lipids are triacylglyceride and cholesterol ester; (iii) the major glycolipids are globotetraosylceramide and globotriaosylceramide; and (iv) the major fatty acids are oleic (18:1), palmitic (16:0), stearic (18:0), and myristic (14:0) acids.

Chlorella vulgaris as a Source of Essential Fatty Acids and Micronutrients: A Brief Commentary

2017 ◽  
Vol 10 (1) ◽  
pp. 92-99 ◽  
Author(s):  
Hércules Rezende Freitas
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Culture Medium ◽  
Essential Fatty Acids ◽  
Total Lipid Content ◽  
Dietary Quality ◽  
Negative Effects ◽  
Algae Biomass ◽  
Dietary Consumption ◽  
Acid Consumption

Polyunsaturated fatty acids (PUFAs) comprise about 35-40% of the total lipid content from green algaeChlorella, reaching up to 24% linoleic acid and 27% α-linolenic acid inC. vulgaris. Also, microalgae nutrient composition may be modulated by changes in the culture medium, increasing fatty acid and microelement concentrations in the algae biomass. PUFAs, such as α-linolenic (n-3) and linoleic (n-6) acids, as well as its derivatives, are considered essential for dietary consumption, and their ability to regulate body chemistry has been recently explored in depth. A balanced fatty acid consumption is shown to counteract the negative effects of western diets, such as chronic inflammation and glucose intolerance. In this brief commentary, technological and practical uses ofC. vulgarisare explored as means to improve dietary quality and, ultimately, human health.

scholarly journals Heat stress elicits remodeling in the anther lipidome of peanut

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Zolian S. Zoong Lwe ◽  
Ruth Welti ◽  
Daniel Anco ◽  
Salman Naveed ◽  
Sachin Rustgi ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Heat Stress ◽  
Fatty Acid ◽  
Membrane Lipids ◽  
Fatty Acid Desaturase ◽  
Susceptible Genotype ◽  
Unsaturated Lipid ◽  
Lipid Species ◽  
Lipid Unsaturation ◽  
Fatty Acid Amount

AbstractUnderstanding the changes in peanut (Arachis hypogaea L.) anther lipidome under heat stress (HT) will aid in understanding the mechanisms of heat tolerance. We profiled the anther lipidome of seven genotypes exposed to ambient temperature (AT) or HT during flowering. Under AT and HT, the lipidome was dominated by phosphatidylcholine (PC), phosphatidylethanolamine (PE), and triacylglycerol (TAG) species (> 50% of total lipids). Of 89 lipid analytes specified by total acyl carbons:total carbon–carbon double bonds, 36:6, 36:5, and 34:3 PC and 34:3 PE (all contain 18:3 fatty acid and decreased under HT) were the most important lipids that differentiated HT from AT. Heat stress caused decreases in unsaturation indices of membrane lipids, primarily due to decreases in highly-unsaturated lipid species that contained 18:3 fatty acids. In parallel, the expression of Fatty Acid Desaturase 3-2 (FAD3-2; converts 18:2 fatty acids to 18:3) decreased under HT for the heat-tolerant genotype SPT 06-07 but not for the susceptible genotype Bailey. Our results suggested that decreasing lipid unsaturation levels by lowering 18:3 fatty-acid amount through reducing FAD3 expression is likely an acclimation mechanism to heat stress in peanut. Thus, genotypes that are more efficient in doing so will be relatively more tolerant to HT.

scholarly journals Lipidomics Provides New Insight into Pathogenesis and Therapeutic Targets of the Ischemia—Reperfusion Injury

2021 ◽  
Vol 22 (6) ◽  
pp. 2798
Author(s):  
Zoran Todorović ◽  
Siniša Đurašević ◽  
Maja Stojković ◽  
Ilijana Grigorov ◽  
Slađan Pavlović ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Time Course ◽  
Membrane Lipids ◽  
Lipid Analysis ◽  
Cell Damage ◽  
Ischemia Reperfusion ◽  
Enzyme Inactivation ◽  
Critical Event ◽  
Tissue Ischemia ◽  
Insight Into

Lipids play an essential role in both tissue protection and damage. Tissue ischemia creates anaerobic conditions in which enzyme inactivation occurs, and reperfusion can initiate oxidative stress that leads to harmful changes in membrane lipids, the formation of aldehydes, and chain damage until cell death. The critical event in such a series of harmful events in the cell is the unwanted accumulation of fatty acids that leads to lipotoxicity. Lipid analysis provides additional insight into the pathogenesis of ischemia/reperfusion (I/R) disorders and reveals new targets for drug action. The profile of changes in the composition of fatty acids in the cell, as well as the time course of these changes, indicate both the mechanism of damage and new therapeutic possibilities. A therapeutic approach to reperfusion lipotoxicity involves attenuation of fatty acids overload, i.e., their transport to adipose tissue and/or inhibition of the adverse effects of fatty acids on cell damage and death. The latter option involves using PPAR agonists and drugs that modulate the transport of fatty acids via carnitine into the interior of the mitochondria or the redirection of long-chain fatty acids to peroxisomes.

scholarly journals Impact of Membrane Lipids on UapA and AzgA Transporter Subcellular Localization and Activity in Aspergillus nidulans

2021 ◽  
Vol 7 (7) ◽  
pp. 514
Author(s):  
Mariangela Dionysopoulou ◽  
George Diallinas
Keyword(s):  
Plasma Membrane ◽  
Subcellular Localization ◽  
Aspergillus Nidulans ◽  
Membrane Lipids ◽  
Membrane Lipid ◽  
Lipid Biosynthesis ◽  
Transport Kinetics ◽  
Negative Effect ◽  
And Function

Recent biochemical and biophysical evidence have established that membrane lipids, namely phospholipids, sphingolipids and sterols, are critical for the function of eukaryotic plasma membrane transporters. Here, we study the effect of selected membrane lipid biosynthesis mutations and of the ergosterol-related antifungal itraconazole on the subcellular localization, stability and transport kinetics of two well-studied purine transporters, UapA and AzgA, in Aspergillus nidulans. We show that genetic reduction in biosynthesis of ergosterol, sphingolipids or phosphoinositides arrest A. nidulans growth after germling formation, but solely blocks in early steps of ergosterol (Erg11) or sphingolipid (BasA) synthesis have a negative effect on plasma membrane (PM) localization and stability of transporters before growth arrest. Surprisingly, the fraction of UapA or AzgA that reaches the PM in lipid biosynthesis mutants is shown to conserve normal apparent transport kinetics. We further show that turnover of UapA, which is the transporter mostly sensitive to membrane lipid content modification, occurs during its trafficking and by enhanced endocytosis, and is partly dependent on autophagy and Hect-type HulARsp5 ubiquitination. Our results point out that the role of specific membrane lipids on transporter biogenesis and function in vivo is complex, combinatorial and transporter-dependent.

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