Comparison of the Modulatory Influence of Maize and Olive Oils and Butter on Metabolic Responses to Endotoxin in Rats

1995 ◽  
Vol 88 (1) ◽  
pp. 59-66 ◽  
Author(s):  
H. T. Besler ◽  
R. F. Grimble
Keyword(s):  
Fatty Acids ◽  
Oleic Acid ◽  
Linoleic Acid ◽  
Olive Oil ◽  
Metabolic Response ◽  
Tissue Protein ◽  
Synthetic Rate ◽  
Zinc Concentrations ◽  
Lung And Kidney ◽  
Maize Oil

1. n-3 polyunsaturated fatty acids decrease responses to cytokines and inflammatory agents. The present study examines how different intakes of n-6 and n-9 fatty acids influence the metabolic response to endotoxin in Wistar rats. 2. Weanling male rats were, for 4 weeks, fed diets containing 50, 100 or 200 g/kg fat in the form of maize oil (rich in linoleic acid), butter (poor in linoleic acid, rich in oleic acid) or olive oil (adequate in linoleic acid, rich in oleic acid) or standard laboratory chow. All butter and olive oil diets included 10 g/kg maize oil, in total fat, to avoid essential fatty acid deficiency. 3. Rats subsequently received 800 μg/kg Escherichia coli endotoxin or sterile saline subcutaneously. Twenty-four hours after injection, the rate of tissue protein synthesis was measured in liver, lung, kidney, tibialis muscle and spleen by the ‘flooding dose’ method. Protein and zinc concentrations were assayed in all tissues and serum albumin and caeruloplasmin measured. 4. In animals fed chow, protein synthetic rate increased by 18%, 29% and 27% in liver, lung and kidney respectively. Tissue zinc concentrations increased by 33% in kidney, and tissue protein increased by 17%, 23% and 17% in liver, lung and kidney respectively. Serum caeruloplasmin increased by 60% and albumin concentration fell by 14%. 5. In animals consuming the 50 g/kg maize oil diet, protein synthetic rate increased by 56%, 36% and 34% in liver, lung and kidney respectively. Tissue zinc concentration increased by 14%, 15% and 17% in the three tissues respectively, and tissue protein concentration increased by 7%, 9% and 51% respectively. Serum caeruloplasmin increased by 172% and albumin concentrations fell by 22%. 6. No change in any parameter occurred in response to endotoxin in rats given diets containing fat predominantly as butter (50 and 100 g/kg), or olive oil (50, 100 and 200 g/kg). 7. In animals fed maize oil diets, responses increased in magnitude in parallel with dietary intake. 8. Responses in rats fed fat (200 g/kg) predominantly as butter were similar to those seen when diets contained 50 g/kg fat as maize oil. 9. The data suggest that the metabolic response to endotoxin is enhanced by n-6 and suppressed by n-9 unsaturated fatty acids. The modulatory influence of fats on responses to inflammatory agents may depend on the relative proportions of these substances.

scholarly journals The incorporation of linoleic acid into the plasma lipids of sheep given intraruminal infusions of maize oil or free linoleic acid

1969 ◽  
Vol 23 (3) ◽  
pp. 709-714 ◽  
Author(s):  
R. C. Noble ◽  
W. Steele ◽  
J. H Moore
Keyword(s):  
Fatty Acids ◽  
Oleic Acid ◽  
Linoleic Acid ◽  
Plasma Lipids ◽  
Cholesteryl Esters ◽  
Absorption Mechanism ◽  
Plasma Triglycerides ◽  
Direct Incorporation ◽  
Maize Oil ◽  
Fatty Acid Compositions

1. The fatty acid compositions of the plasma cholesteryl esters, phospholipids, triglycerides and unesterilied fatty acids were determined in two sheep at various times after they had been given intraruminal infusions of emulsions of maize oil or linoleic acid.2. The concentration of linoleic acid in the plasma triglycerides began to increase 3 h after infusion began. The infusions of maize oil and linoleic acid resulted in the appearance of peak concentrations of linoleic acid in the plasma triglycerides 6 and 12h respectively after infusion began.3. After the infusion of maize oil the plasma triglycerides showed an increasein theconcentration of stearic acid but after the infusion of linoleic acid the plasma triglycerides showed an increase in the concentration of oleic acid.4. The concentration of linoleic acid in the plasma phospholipids and cholesteryl esters did not begin to increase until 6–9 h and 24–25 h respectively after the infusions had begun.5. No evidence was found for an absorption mechanism which involved the direct incorporation of linoleic acid into the blood phospholipids or cholesteryl esters.

scholarly journals The incorporation of linolenic and linoleic acids into the plasma lipids of sheep given intra-abomasal infusions of linseed oil, maize oil or linoleic acid

1969 ◽  
Vol 23 (1) ◽  
pp. 141-152 ◽  
Author(s):  
J. H. Moore ◽  
R. C. Noble ◽  
W. Steele
Keyword(s):  
Fatty Acids ◽  
Small Intestine ◽  
Oleic Acid ◽  
Linoleic Acid ◽  
Plasma Lipids ◽  
Linseed Oil ◽  
Cholesteryl Esters ◽  
Plasma Triglycerides ◽  
Maize Oil ◽  
Fatty Acid Compositions

1. The fatty acid compositions of the plasma cholesteryl esters, phospholipids, triglycerides and unesterified fatty acids were determined in three sheep at various times after they had been given intra-abomasal infusions of emulsions of linseed oil, maize oil or linoleic acid.2. The concentrations of linolenic acid or linoleic acid in the plasma triglycerides began to increase 1.5 h after infusion of the emulsions had begun. As the concentration of linolenic or linoleic acids in the plasma triglycerides increased, the concentrations of palmitic and stearic acids decreased, hut there were no appreciable changes in the concentrations of oleic acid.3. The concentrations of linolenic or linoleic acid in the plasma phospholipids and cholesteryl esters did not begin to increase until 8–9 h and 24–25 h respectively after the infusions of the emulsions had begun.4. It is suggested that, after absorption from the small intestine of the sheep, linolenic and linoleic acids are transported in triglyceride form to the liver where the triglycerides are partially or completely hydrolysed. These C18 polyunsaturated acids are then preferentially utilized for the synthesis of phospholipids and cholesteryl esters but not for the re-synthesis of triglycerides.

scholarly journals Effect of Vegetable Oil Fortified Feeds on the Content of Fatty Acids in Breast and Thigh Muscles in Broiler Chickens

2010 ◽  
Vol 79 (9) ◽  
pp. S21-S28 ◽  
Author(s):  
Tereza Krejčí-Treu ◽  
Eva Straková ◽  
Pavel Suchý ◽  
Ivan Herzig
Keyword(s):  
Fatty Acids ◽  
Oleic Acid ◽  
Linoleic Acid ◽  
Soybean Oil ◽  
Olive Oil ◽  
Linolenic Acid ◽  
Vegetable Oils ◽  
Broiler Chickens ◽  
Flaxseed Oil ◽  
Thigh Muscles

The main objective of this work was to compare the effect of six vegetable oils added to feeding mixtures that were administered to broiler chickens on the content of major fatty acids in chicken meat. The experiment started with 90 one-day-old Ross 308 meat hybrid male chickens that were divided into six groups. Chickens were fed complete feeding mixtures for the prefattening (BR1), fattening (BR2), and post-fattening (BR3) of broiler chickens. The BR1 feeding mixture was administered to chickens aged 1-10 days, the BR2 feeding mixture was given from Day 11 to Day 30, and the BR3 feeding mixture was then administered until Day 42. The BR1 feeding mixture that was administered to all six groups during the first ten days of the experiment was supplemented with soybean oil. BR2 and BR3 feeding mixtures used to feed chickens aged 11-42 days were fortified with soybean oil (SO Group), rapeseed oil (RO Group), sunflower oil (SFO Group), flaxseed oil (FO Group), olive oil (OO Group), and evening primrose oil (EPO Group). The vegetable oils used differed by the composition of fatty acids, particularly by the content of oleic acid, linoleic acid, α-linolenic acid. The use of the above-described experimental diets in young broilers from Day 11 to 42 had a significant effect on the content of fatty acids in the fat from breast and thigh muscles. The content of α-linolenic acid in breast and thigh muscles of broilers that received the feed containing flaxseed oil (21.16 g/100 g of oil and 17.13 g/100 g of oil, respectively) significantly increased (p ⪬ 0.01). The highest content of linoleic acid (p ⪬ 0.01) in breast and thigh muscles was found in chickens that were fed the feed containing primrose oil (59.13 g/100 g and 51.71 g/100 g). A significant increase (p ⪬ 0.01) in the level of oleic acid was detected in both breast and thigh muscles of broilers that received olive oil fortified feed (52.44 g/100 g and 43.70 g/100 g of oil). No significant variation was found in the content of palmitic acid and palmitooleic acid. The levels of oleic acid, linoleic acid and α-linolenic acid in feeding mixtures correlated with those found in breast and thigh muscles (r = 0.88; 0.94 and 0.99; r = 0.99; 0.98 and 0.99).

scholarly journals Studying the Physicochemical Properties and Isolation of Unsaturated Fatty Acids from Edible Oils by GC-MS and Argentated Silica Gel Chromatography

2021 ◽  
pp. 346-362
Author(s):  
Salim Najmaldain Saber ◽  
Hikmat Ali Mohamad ◽  
Madzlan Aziz
Keyword(s):  
Fatty Acids ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Methyl Ester ◽  
Physicochemical Properties ◽  
Olive Oil ◽  
Unsaturated Fatty Acids ◽  
Acid Methyl Ester ◽  
Acid Methyl

The core objective of this study was to investigate the physicochemical characteristics and fatty acid composition of the oils of sunflower, olive, virgin coconut and ginger oils, as well as the separation of their unsaturated fatty acids. The data indicated a significant variation in physicochemical properties (acid, saponification, ester, and iodine values) among oils. Transesterification process was carried out at a molar ratio of 1:7:0.1 of oil: methanol: KOH. Fatty acid methyl esters of oils were analyzed by infrared (IR) and gas chromatography–mass (GC-MS) spectrometry. Twelve fatty acids were identified, where the major fatty acid of  olive oil was found to be  oleic acid (89%), whereas those of sunflower and ginger oils were linoleic acid (80.9 %) and (79.3 %), respectively. Sunflower and olive oils were fractionated by 25% silver nitrate-impregnated silica gel column chromatography. By this method, linoleic acid methyl ester from sunflower and oleic acid methyl ester from olive oil were isolated with high purity percentages and yields. This study is significant for the development of food and pharmaceutical products.

scholarly journals Saurani Zeytinyağinin Bazi Kalite Parametreleri Üzerine Zeytin Olgunluğunun Etkisi

2022 ◽  
Vol 9 (sp) ◽  
pp. 2493-2497
Author(s):  
Dilşat Bozdoğan Konuşkan
Keyword(s):  
Fatty Acids ◽  
Oleic Acid ◽  
Linoleic Acid ◽  
Free Fatty Acids ◽  
Olive Oil ◽  
Acid Content ◽  
Arachidic Acid ◽  
Soil Conditions ◽  
Oleic Acid Content ◽  
Olive Variety

There are 97 local olive varieties registered in our country, and Saurani is an olive variety from originating Hatay/Altınözü. Hatay province, which ranks first in olive production in the Mediterranean Region, has very suitable climate and soil conditions for olive cultivation. The aim of this study is to investigate the effect of olive maturity on quality parameters such as free fatty acids, peroxide value and fatty acid composition of the oil obtained from the Saurani olive variety grown in Hatay. For this purpose, olive oil was obtained by mechanical method from olives collected from Saurani variety in 3 different maturity periods of the 2020 production season. In Saurani olive oil, free fatty acids and peroxide numbers were found in the range of 0.62-0.91 % (oleic acid) and 3.68-5.26 meq O2/kg respectively. The amount of free fatty acids increased with maturity. In Saurani olive oil were determined oleic acid in the range of 66.32%-68.79%, palmitic acid in the range of12.47-13.75%, linoleic acid in the range of 11.43-13.84%, stearic acid in the range of 3.16-3.42%, palmitoleic acid 1.12%-1.34%, linolenic acid 0.88-1.01% and arachidic acid 0.41-0.52. It was determined that decrease in oleic acid content and an increase in linoleic acid content with maturity. It has been determined that Saurani olive oil is within the limits specified in the Turkish Food Codex on Olive Oil and Pirina Olive Oil in terms of the examined properties.

scholarly journals Energy intake, gastrointestinal transit, and gut hormone release in response to oral triglycerides and fatty acids in men with and without severe obesity

2019 ◽  
Vol 316 (3) ◽  
pp. G332-G337 ◽  
Author(s):  
Carsten Dirksen ◽  
Jesper Graff ◽  
Stefan Fuglsang ◽  
Jens F. Rehfeld ◽  
Jens J. Holst ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Oleic Acid ◽  
Olive Oil ◽  
Energy Intake ◽  
Severe Obesity ◽  
Gastrointestinal Transit ◽  
Calorie Intake ◽  
Double Blind ◽  
Gut Hormone ◽  
Ad Libitum

Dietary fat, and particularly fatty acids (FAs) from hydrolyzed triglycerides (TGs), reduces appetite, whereas paradoxically, a high-fat diet leads to excess calorie intake. We therefore hypothesized that the appetite-regulating effects of FAs are perturbed in obesity. Ten men with severe obesity [median body mass index (BMI) of 51.0 kg/m2(range of 47.9–69.0)] and 10 men without obesity [BMI of 24.6 kg/m2(range of 21.7–26.8)] were recruited for a double-blind randomized crossover study. On two occasions, participants were given isocaloric (2,660 kJ) and isovolemic (80 ml) loads of either oleic acid (long-chain FA) or olive oil (TG) containing radiolabeled lipid and water markers. Postload scintigraphy, blood sampling, and assessment of appetite were performed for 10 h, after which an ad libitum meal was served. Compared with olive oil, oleic acid slowed gastric mean emptying time (GMET) for lipids ( P < 0.001), accelerated orocoecal transit time (OCTT; P = 0.005), increased postload cholecystokinin section ( P < 0.001), and suppressed ad libitum energy intake ( P = 0.028) in men with severe obesity, and similar effects were seen in the nonobese group (no group × lipid interactions). However, independent of lipid loads, GMET and OCTT were slower (GMETlipidP = 0.046; GMETwaterP = 0.003; OCTT P = 0.001), and basal and postload secretion of glucagon-like peptide-1 (GLP-1) was attenuated ( P = 0.045 and P = 0.048, respectively) in men with severe obesity compared with men without obesity. We conclude that the more potent appetite-regulating effects of oleic acid versus olive oil are unimpaired in men with severe obesity. However, regardless of lipid formulations, severe obesity is associated with slowed gastrointestinal transit and attenuated GLP-1 secretion.NEW & NOTEWORTHY Orally ingested fatty acids more efficiently reduce appetite and energy intake than triglycerides also in men with severe obesity. Men with severe obesity have delayed gastrointestinal transit and attenuated early gut hormone responses after an oral lipid load compared with men without obesity.

scholarly journals Inhibition of steroid 5α-reductase by specific aliphatic unsaturated fatty acids

1992 ◽  
Vol 285 (2) ◽  
pp. 557-562 ◽  
Author(s):  
T Liang ◽  
S Liao
Keyword(s):  
Fatty Acids ◽  
Oleic Acid ◽  
Linoleic Acid ◽  
Linolenic Acid ◽  
Unsaturated Fatty Acids ◽  
Binding Assay ◽  
Reductase Activity ◽  
Undecylenic Acid ◽  
Gamma Linolenic Acid ◽  
Enzymic Assay

Human or rat microsomal 5 alpha-reductase activity, as measured by enzymic conversion of testosterone into 5 alpha-dihydrotestosterone or by binding of a competitive inhibitor, [3H]17 beta-NN-diethulcarbamoyl-4-methyl-4-aza-5 alpha-androstan-3-one ([3H]4-MA) to the reductase, is inhibited by low concentrations (less than 10 microM) of certain polyunsaturated fatty acids. The relative inhibitory potencies of unsaturated fatty acids are, in decreasing order: gamma-linolenic acid greater than cis-4,7,10,13,16,19-docosahexaenoic acid = cis-6,9,12,15-octatetraenoic acid = arachidonic acid = alpha-linolenic acid greater than linoleic acid greater than palmitoleic acid greater than oleic acid greater than myristoleic acid. Other unsaturated fatty acids such as undecylenic acid, erucic acid and nervonic acid, are inactive. The methyl esters and alcohol analogues of these compounds, glycerols, phospholipids, saturated fatty acids, retinoids and carotenes were inactive even at 0.2 mM. The results of the binding assay and the enzymic assay correlated well except for elaidic acid and linolelaidic acid, the trans isomers of oleic acid and linoleic acid respectively, which were much less active than their cis isomers in the binding assay but were as potent in the enzymic assay. gamma-Linolenic acid had no effect on the activities of two other rat liver microsomal enzymes: NADH:menadione reductase and glucuronosyl transferase. gamma-Linolenic acid, the most potent inhibitor tested, decreased the Vmax. and increased Km values of substrates, NADPH and testosterone, and promoted dissociation of [3H]4-MA from the microsomal reductase. gamma-Linolenic acid, but not the corresponding saturated fatty acid (stearic acid), inhibited the 5 alpha-reductase activity, but not the 17 beta-dehydrogenase activity, of human prostate cancer cells in culture. These results suggest that unsaturated fatty acids may play an important role in regulating androgen action in target cells.

scholarly journals CONTENT OF FATTY ACIDS IN CORN (ZEA MAYS L.) OIL FROM ECUADOR

2017 ◽  
Vol 10 (8) ◽  
pp. 150 ◽  
Author(s):  
Carrillo W ◽  
Carpio C ◽  
Morales D ◽  
Vilcacundo E ◽  
Álvarez M ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Zea Mays ◽  
Oleic Acid ◽  
Linoleic Acid ◽  
Corn Oil ◽  
Methyl Esters ◽  
Total Content ◽  
Pressing Method ◽  
A Value ◽  
Omega 6

  Objective: The aim of this work was to determine the fatty acids content in corn seeds oil (Zea mays) sample cultivated in Ecuador.Methods: Corn oil was obtained from corn oil seeds using the cold pressing method. Methyl esters fatty acids analysis were carried out using the gas chromatography (GC) method with a mass selective detector and using the database library NIST 14.L to identify the compounds present in the corn seed oil.Results: Methyl esters fatty acids were identified from corn (Z. mays) seeds using the GC mass spectrometer (GC-MS) analytical method. Fatty acids were analyzed as methyl esters on a capillary column DB-WAX 122-7062 with a good separation of palmitic acid, stearic acid, oleic acid, elaidic acid, linoleic acid, arachidic acid, and linolenic acid. The structure of methyl esters fatty acids was determined using the GS-MS method. Corn oil has a high content of linoleic acid (omega 6) with a value of 52.68% of the total content of fatty acids in corn oil and 29.70% of oleic acid (omega 9) of the total content of fatty acids in corn oil. The sample presented a value of 12.57% of palmitic acid.Conclusions: Corn oil shows a good content of fatty acids omega 6 and 9. The higher value was of omega 6 with 52.68% content. Corn oil has a good proportion of polyunsaturated of lipids (53.80%) and 14.86% of saturated lipids.

scholarly journals Bioinformatics exploration of olive oil: molecular targets and properties of major bioactive constituents

OCL ◽  
2021 ◽  
Vol 28 ◽  
pp. 36
Author(s):  
Toluwase Hezekiah Fatoki ◽  
Cecilia O. Akintayo ◽  
Omodele Ibraheem
Keyword(s):  
Oleic Acid ◽  
Linoleic Acid ◽  
Olive Oil ◽  
Oleanolic Acid ◽  
Tyrosine Phosphatase ◽  
Fatty Acid Binding Proteins ◽  
Protein Tyrosine Phosphatase 1B ◽  
Bioactive Constituents ◽  
Fatty Acid Binding ◽  
Maslinic Acid

Olive oil possesses medicinal properties which include antimicrobial, antioxidant and anti-inflammatory, anti-diabetes, and anti-cardiovascular diseases. Oleic acid is the most abundant (95%) constituent of olive oil and others include linoleic acid, oleuropein, oleanolic acid, maslinic acid, melatonin, and others. The objective of this study is to predict the molecular targets and properties of key bioactive components of olive oil in human. Bioinformatics methods, which involved pharmacokinetics prediction, target prediction and gene network analyses, were used. The results showed that oleic acid has similar targets with linoleic acid, and showed significant probability of binding to several targets such as fatty acid-binding proteins in the adipose, epidermal, liver and muscle as well as alpha, delta and gamma peroxisome proliferator-activated receptors (PPARs). Carbonic anhydrase showed to be the only significant target of tyrosol, while protein-tyrosine phosphatase 1B, and CD81 antigen were targeted by maslinic acid and oleanolic acid. This study has applauded oleic acid, linoleic acid and tyrosol as olive oil bioactive constituents that have several potential pharmacological effects in humans that modulate several enzymes, receptors and transcription factors. The future work will be to investigate the effects of oleic acid on fatty acid-binding proteins and telomerase reverse transcriptase; melatonin on quinone reductase 2; tyrosol on carbonic anhydrase II; maslinic acid and oleanolic acid on protein-tyrosine phosphatase 1B.

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