scholarly journals Possibilities of using the HPLC method in the taxonomy of the genus Nitraria (Nitrariaceae)

2020 ◽  
Vol 24 ◽  
pp. 00096
Author(s):  
Maria Voronkova ◽  
Evgeniy Banaev ◽  
Maria Tomoshevich ◽  
Taigana AK-Lama
Keyword(s):  
High Performance Liquid Chromatography ◽  
Phenolic Compounds ◽  
High Performance ◽  
Component Composition ◽  
Hplc Method ◽  
Multidimensional Analysis ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Specific Composition ◽  
Species Specific

The paper shows composition and content of phenolic compounds in leaves of 4 Nitraria L. species from 58 populations of Russia, Kazakhstan, and Tajikistan studied with high-performance liquid chromatography(HPLC). The investigation has revealed 27 compounds of phenolic nature: the maximum number (18 components) is detected in leaves of N. sibirica Pall. from Kazakhstan (the Karatal river valley), 16-17 components – in plants from three populations of Siberia, the minimum (6 components) – in leaves of N. komaroviiilljin & Lava ex Bobrov. The analysis has identified hyperoside (quercetin O-glycoside), narcissin (isoramnetin O-glycoside), quercetin (flavonol) and luteolin (flavon). The studied plants accumulate a generous quantity of phenolic compounds. Their content reaches 4.64% in leaves of N. sibirica, 3.11% – in N. schoberi L., up to 3.96% – in N. komarovii. The research results allow speaking about the species-specific composition and content of phenolic compounds of N. sibirica, N. schoberi and N. komarovii. The component composition is weaker in extracts of N. komarovii leaves, but there is a higher content of total phenolic compounds compared to N. schoberi plants. N. pamirica L. Vassil sample is close to N. schoberi on multidimensional analysis of the phenolic compound composition and content.

scholarly journals COMPARATIVE STUDY OF PHENOLIC COMPOUNDS COMPOSITION AND CONTENT IN LEAVES OF THE GE-NUS NITRARIA (NITRARIACEAE) PLANTS

2017 ◽  
pp. 107-116
Author(s):  
Мария (Marija) Сергеевна (Sergeevna) Воронкова (Voronkova) ◽  
Евгений (Evgenij) Викторович (Viktorovich) Банаев (Banaev) ◽  
Мария (Marija) Анатольевна (Anatol'evna) Томошевич (Tomoshevich)
Keyword(s):  
High Performance Liquid Chromatography ◽  
Multivariate Analysis ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Seasonal Dynamics ◽  
High Performance ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Flowering Stage ◽  
Study Results

The content and composition of phenolic compounds in leaves of 3 Nitraria L. species from 12 populations have been studied with a high-performance liquid chromatography (HPLC). The investigation has detected 17 phenolic compounds, the maximum number of components is 12 indicated in leaves of N. pamirica L. Vassil. and N. schoberi L. plants in 2 populations, the minimum – 6 components – has been revealed in leaves of N. komarovii Iljin et Lava. Hyperoside (O-glycoside of quercetin), narcissin (O-glycoside of isoramnetin), quercetin (flavonol) and luteolin (flavon) have been identified. Studied plants accumulate a significant amount of phenolic compounds. Their content reaches 2,57% in leaves of N. schoberi L., 3,96% in N. komarovii. It was marked seasonal dynamics of a number of substances, in particular, lower narcissin content during plants fruiting compared to a flowering stage. The study results allow making an assumption of species peculiarities of phenolic compounds composition and content in N. schoberi and N. komarovii. The components composition is poorer in extracts of N. komarovii leaves, however, they shows higher content of total phenolic compounds compared with N. schoberi plants. At the same time N. pamirica sample is close to N. schoberi one based on a multivariate analysis of phenolic compounds composition and content.

scholarly journals Seasonal Variation of Health-Promoting Bioactives in Broccoli and Methyl-Jasmonate Pre-Harvest Treatments to Enhance Their Contents

Foods ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1371 ◽  
Author(s):  
Vanesa Nuñez-Gómez ◽  
Nieves Baenas ◽  
Inma Navarro-González ◽  
Javier García-Alonso ◽  
Diego A. Moreno ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Methyl Jasmonate ◽  
Bioactive Compounds ◽  
High Performance ◽  
Total Phenolic ◽  
Diode Array ◽  
Total Carotenoids ◽  
Health Promoting

Broccoli is a source of bioactive compounds that provide an important nutritional value. The content of these compounds can vary depending on agronomic and environmental conditions, as well as on elicitation. In this study, three crop trials were carried out to evaluate the effects of the cultivation season, the application of different dosages of methyl-jasmonate (MeJA) on the overall quality and on the total content of bioactive compounds of ‘Parthenon’ broccoli cultivated under the field conditions of southeastern Spain. Color parameters, chlorophyll content, total phenolic compounds, total flavonoids and antioxidant activity were measured to evaluate the overall quality. Moreover, individual carotenoids, phenolic compounds and glucosinolates were evaluated by high performance liquid chromatography with diode array detection (HPLC-DAD) and high performance liquid chromatography equipped with diode array detector coupled to mass spectrometer using electro spray ionization (HPLC-DAD-ESI/MSn). The content of total carotenoids, phenolic compounds and glucosinolates were higher in autumn compared with spring, showing increases of 2.8-fold, 2-fold and 1.2-fold, respectively. Moreover, a double application of MeJA increased the contents of total carotenoids, phenolic compounds and glucosinolates by 22%, 32% and 39%, respectively, relative to the untreated samples. Considering our results, the controlled and timely application of 250 µM MeJA to the aerial parts of the plants four days before harvest, on two consecutive days, seems to be a valid agronomic strategy to improve the health-promoting capacity of Parthenon broccoli, without compromising its overall quality.

scholarly journals Schisandra rubriflora Plant Material and In Vitro Microshoot Cultures as Rich Sources of Natural Phenolic Antioxidants

Antioxidants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 488
Author(s):  
Agnieszka Szopa ◽  
Michał Dziurka ◽  
Sebastian Granica ◽  
Marta Klimek-Szczykutowicz ◽  
Paweł Kubica ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Phenolic Acids ◽  
High Performance ◽  
Total Phenolic ◽  
Array Detector ◽  
Plant Materials

Schisandra rubriflora is a dioecious, underestimated medicinal plant species known from traditional Chinese medicine. The present study was aimed at characterising the polyphenolic profile composition and the related antioxidant capacity of S. rubriflora fruit, stem and leaf and in vitro microshoot culture extracts. Separate analyses of material from female and male specimens were carried out. This study was specifically aimed at detailed characterisation of the contribution of phenolic compounds to overall antioxidant activity using ultra-high-performance liquid chromatography with a photodiode array detector coupled to electrospray ionization ion trap mass spectrometry (UHPLC-DAD-ESI-MS3) and a high-performance liquid chromatography-diode array detector (HPLC-DAD). Using UHPLC-DAD-ESI-MS3, twenty-seven phenolic compounds from among phenolic acids and flavonoids were identified. Concentrations of three phenolic acids (neochlorogenic, chlorogenic and cryptochlorogenic acids) and eight flavonoids (hyperoside, rutoside, isoquercitrin, guaijaverin, trifolin, quercetin, kaempferol, and isorhamnetin) were determined using HPLC-DAD using reference standards. The highest total phenolic content was confirmed for the stem and leaf extracts collected in spring. The contents of phenolic compounds of in vitro biomasses were comparable to that in the fruit extracts. The methanolic extracts from the studied plant materials were evaluated for their antioxidant properties using various in vitro assays, namely free radicals scavenging estimation using 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH), ferric-reducing antioxidant power (FRAP) and cupric-reducing antioxidant capacity (CUPRAC) as well as QUick, Easy, New, CHEap, and Reproducible CUPRAC (QUENCHER-CUPRAC) assays. A close relationship between the content of polyphenolic compounds in S. rubriflora and their antioxidant potential has been documented.

scholarly journals Phenolic compounds of Bassia prostrata (Chenopodiaceae)

2021 ◽  
Vol 38 ◽  
pp. 00097
Author(s):  
Anastasia Petruk ◽  
Tatyana Pankova ◽  
Bektemir Osmonali ◽  
Maria Lomonosova
Keyword(s):  
High Performance Liquid Chromatography ◽  
Phenolic Compounds ◽  
High Performance ◽  
Component Composition ◽  
Qualitative And Quantitative ◽  
Quantitative Content ◽  
Novosibirsk Region ◽  
Polymorphic Species ◽  
Double Extraction ◽  
Different Populations

The composition and content of phenolic compounds in plants of the polymorphic species Bassia prostrata (Chenopodiaceae) from geographically distant populations were studied by high-performance liquid chromatography. The material for the study was the aboveground part of the plant. The plants were collected in the flowering phase in Russia (Novosibirsk region, Republic of Tyva, Khakassia, Buryatia, Altai), Kazakhstan, Armenia. The phenolic compounds were extracted by double extraction with 70% ethanol. Component composition of the phenolic complex was investigated by Agilent 1200 chromatograph. Eleven phenolic compounds, including isoquercitrin, kaempferol-3-glycoside, isoramnetin-3-rutinoside, and luteolin were found in the composition. The quantitative content of each compound could vary from 0.1 to 10.8 mg/g in different populations. Chemotypes were determined for the qualitative and quantitative content of phenolic compounds

scholarly journals Study on antioxidant activity of crude peanut oils and refined cottonseed oils from Burkina Faso

2021 ◽  
Vol 6 (4) ◽  
pp. 920-931
Author(s):  
Souleymane Zio ◽  
◽  
Bakary Tarnagda ◽  
Flibert Guira ◽  
Driss Elothmani ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Burkina Faso ◽  
Vegetable Oils ◽  
High Performance ◽  
Edible Oils ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Nutrient Sources ◽  
Feeding Programs

<abstract> <p>Vegetable oils are among the foodstuffs produced and consumed in abundance by the population in Burkina Faso. These edible oils are nutrient sources for health. However, the oxidation of edible oils is a phenomenon that leads to their degradation. Hence, the objectives of this study were to determine the antioxidants compounds, to evaluate the antioxidant activity of vegetable oils produced in Burkina Faso and to propose good manufacturing practices. Thus, 32 samples of crude peanut oils and refined cottonseed oils were analyzed. α-Tocopherol was determined by High performance liquid chromatography (HPLC), total phenolic compounds and DPPH by spectrophotometry. The α-tocopherol averages are 10.89 and 56.44 mg/100 g for peanut oils and cottonseed oils, respectively (<italic>p</italic> &lt; 0.05). For total phenolic compounds, the averages are 2.91 and 0.64 mg/100 g of gallic acid equivalent for peanut oils and cottonseed oils, respectively (<italic>p</italic> &lt; 0.05). The inhibition percentages are respectively 17.97% and 5.58% (<italic>p</italic> &lt; 0.05) for peanut oils and cottonseed oils. For antioxidant activity, the averages are 0.81 and 0.27 mg trolox/100 g for peanut oils and cottonseed oils, respectively (<italic>p</italic> &lt; 0.05). Cottonseed oils have the highest levels of α-tocopherol while peanut oils have high levels of total phenolic compounds and antioxidant activity. Vegetable oils contain acceptable levels of α-tocopherol and total phenolic compounds for their oxidative stability and health benefits for the consumer. However, the levels of biomolecules will be higher if production and preservation conditions are improved and controlled. The results could be used for future recommendations about human feeding programs in Burkina Faso.</p> </abstract>

scholarly journals THE EFFECT OF TEMPE FERMENTATION TIME ON THE TOTAL PHENOLIC AND ISOFLAVONE GENISTEIN CONTENTS

2017 ◽  
Vol 13 (2) ◽  
pp. 228
Author(s):  
Kiki Fransiska Suharto ◽  
Hartati Soetjipto ◽  
Yohanes Martono
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
Block Design ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Fermentation Time ◽  
Significance Level ◽  
Phenolic Contents ◽  
Honestly Significant Difference ◽  
Significant Difference

<p>The objective of this study was to evaluate total phenolic compounds and isoflavone genistein contents during 0 - 9 days of fermentation time. Isoflavone extract were obtained by maceration and fractionation. Total phenolic compounds were measured by Folin ciocalteau method, meanwhile, the isoflavone genistein contents were analyzed by a High Performance Liquid Chromatography. The yield of isoflavone extract and the total phenolic compounds were analyzed using a randomized completely block design and the mean between treatments was compared by the Honestly Significant Difference (HSD) test using significance level of 5 %. The highest amount of total phenolic compounds during the incubation time was obtained on 4 days of fermentation time, i.e., 232.05n ± 7.71 μg/g, while the highest content of isoflavone genistein was obtained on 5 days of fermentation time, i.e., 100.48 μg/g. This research confirms that the fermentation process of tempe induces the total phenolic contents and the production of isoflavone genistein is fluctuating.</p>

Identification and Quantification of Phenolic Compounds in Grains of Biofortified Cowpea Cultivars, Before and After Cooking

2020 ◽  
Vol 16 (1) ◽  
pp. 105-113
Author(s):  
Nara Vanessa dos Anjos Barros ◽  
Bruna Barbosa de Abreu ◽  
Débora Thaís Sampaio da Silva ◽  
Ana Karine de Oliveira Soares ◽  
Maurisrael de Moura Rocha ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
High Performance ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Health Maintenance ◽  
Cowpea Cultivars ◽  
Genetically Improved ◽  
Before And After ◽  
Ferulic Acids

Objective: This study aimed to identify and quantify phenolic compounds in the grains of biofortified cowpea (Vigna unguiculata) cultivars before and after cooking. Methods: We analyzed two cultivars of genetically improved cowpeas, namely BRS Aracê and BRS Tumucumaque. Raw and cooked samples of the cultivars were analyzed (in triplicate). The concentrations of phenolics, flavonoids, anthocyanins, and flavanols, as well as the antioxidant activity, were determined. Phenolic compounds were identified and quantified using high-performance liquid chromatography. Results: Grains of cultivar BRS Tumucumaque had higher concentrations of total phenolic compounds before (297.23 ± 4.24 (mean ± standard deviation) mg/100 g) and after (147.15 ± 6.94 mg/100 g) cooking, and higher concentrations of total flavonoids before (49.36 ± 2.02 mg/100 g) and after (23.97± 0.67 mg/100 g) cooking. Anthocyanins were not measured in the grains of either cultivars. There was a statistically significant reduction in the concentration of total flavanols after cooking, with the greater retention in BRS Aracê. Similarly, the antioxidant activity was significantly reduced after cooking, with greater reductions in BRS Tumucumaque than in BRS Aracê. Five phenolic acids were identified and quantified, including gallic, caffeic, and ferulic acids. Conclusion: We conclude that cooking affected the concentrations of phenolic compounds in the cultivars, as well as the antioxidant activity exhibited by these cultivars. The cultivars contained beneficial compounds that can contribute to disease prevention and health maintenance.

scholarly journals Фенольні сполуки листя Sorbus domestіca і Sorbus graeca

2018 ◽  
Author(s):  
O. V. Krivoruchko
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Chlorogenic Acid ◽  
High Performance ◽  
Hplc Method ◽  
Chromatography Method ◽  
Pharmacological Studies ◽  
Rosaceae Family ◽  
Liquid Chromatography Method

Introduction. Sorbus domestіca and Sorbus graeca from the Rosaceae family are cultivated in Ukraine in gardens and parks. Their chemical composition is investigated insufficiently.The aim of the study – to research the composition and the content of phenolic compounds of leaves of Sorbus domestіca and Sorbus graeca.Research Methods. The content of phenolic compounds in leaves of Sorbus domestіca and Sorbus graeca was carried out by the high performance liquid chromatography method on Agilent Technologies chromatograph.Results and Discussion. In leaves of Sorbus domestіca chlorogenic acid, rutin, quercetin-3-O-glucoside and quercetin were identified; in leaves of Sorbus graeca chlorogenic and neochlorogenic acids, rutin and 4'-methoxyquercetin-3-O-sophoroside were identified. The content of hydroxycinnamic acids in leaves of Sorbus domestіca is 389 mg/100 g, in leaves of Sorbus graeca – 147 mg/100 g (in terms of chlorogenic acid). The content of flavonoids in leaves of Sorbus domestіca is 1888 mg/100 g, in leaves of Sorbus graeca – 727 mg/100 g (in terms of rutin).Conclusions. The composition and the content of phenolic compounds of Sorbus domestіca and Sorbus graeca were studied by the HPLC method. Leaves of Sorbus domestіca are more perspective for further pharmacological studies.

scholarly journals Development of HPLC method for determination of phenolic compounds on a core shell column by direct injection of wine samples

2020 ◽  
Vol 32 (2) ◽  
pp. 134-138
Author(s):  
Milica Atanacković Krstonošić ◽  
Jelena Cvejić Hogervorst ◽  
Mira Mikulić ◽  
Ljiljana Gojković-Bukarica
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
High Performance ◽  
Direct Injection ◽  
Syringic Acid ◽  
Hplc Method ◽  
Core Shell ◽  
Validation Parameters

Phenolic compounds are frequently present in various natural products, and they can have different beneficial biological potentials. The most widely used method for determination of individual phenolic compounds is high-performance liquid chromatography (HPLC). In this paper, a method for simultaneous determination of 16 phenolic compounds (gallic acid, p-hydroxybenzoic acid, catechin, syringic acid, trans-cinnamic acid, hesperetin, naringenin, vanillic acid, benzoic acid, coumaric acid, resveratrol, chlorogenic acid, caffeic acid, rutin, quercetin, and kaempferol) on a core–shell column was developed. The separation method conducted on a standard ODS (250 mm) column was transferred to Poroshell column and optimized using non-ultra-high-performance liquid chromatography (UHPLC) apparatus. Phenolic compounds were separated fast and efficiently during 30-min analysis, and validation parameters were determined. The developed method was successfully applied on the analysis of phenolic content after direct injection of red wines from three different grape varieties.

Analisis Kadar Zat Pewarna Tartrazin pada Minuman Ringan Berkarbonasi Khas Pekalongan dengan Metode High Performance Liquid Chromatography (HPLC)

2021 ◽  
Vol 1 ◽  
pp. 685-691
Author(s):  
AT Meta Lansamigi ◽  
Achmad Vandian Nur ◽  
W Wirasti ◽  
Khusna Santika Rahmasari
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Mobile Phase ◽  
High Performance ◽  
Hplc Method ◽  
Lemon Yellow ◽  
Colour Reaction ◽  
Auc Value ◽  
Carbonated Drinks

AbstractTartrazine is a coloring agent which is a mixture of phenolic compounds, polycyclic hydrocarbons, and heterocyclics. Because it dissolves easily in water, tartrazine is commonly used as a coloring agent in beverages. The addition of tartrazine in the drink aims to give it a more attractive color, namely lemon yellow. The purpose of this study was to analyze the tartrazine content in carbonated drinks and to determine whether the tartrazine content in the sample was in accordance with the BPOM Regulation No. 11 of 2019. Qualitative testing using colour reaction with FeSO4. Quantitative testing using the high performance liquid chromatography (HPLC) method with a mobile phase of acetonitrile, methanol and aquabides with a ratio (65,5 : 23,5 : 11). The data obtained is the AUC value. Data analisys using linear regression. The resul of qualitatif analysis with acolor test using FeSO4 reagent which produces a color until it is cloudy and is present on the surface. While the quanitative analysis using HPLC obtained the average levels in the oranfe sample 1,109 mg/kg and pineapple sample 0,933 mg/kg.Key words: Analysis, levels, tartrazine, dye, HPLC AbstrakTartrazin merupakan zat pewarna campuran dari senyawa fenol, hidrokarbon polisiklik, dan heterosiklik. Pada umumnya tartrazin digunakan sebagai bahan pewarna minuman karena mudah larut dalam air. Penambahan tartrazin dalam minuman bertujuan untuk memberikan warna yang lebih menarik yaitu kuning lemon. Tujuan dari penelitian ini adalah untuk menganalisis kandungan tartrazin dalam minuman berkarbonasi dan untuk mengetahui apakah kadar tartrazin dalam sampel sudah sesuai dengan peraturan BPOM No.11 Tahun 2019. Pengujian secara kualitatif dengan metode uji warna menggunakan FeSO4. Pengujian secara kuantitatif menggunakan metode High Performance Liquid Chromatography (HPLC) dengan fase gerak asetonitril, metanol, dan aquabides dengan perbandingan (65,5 : 23,5 : 11). Data yang didapat adalah nilai AUC. Analisis data menggunakan regresi linier. Hasil dari analisis kualitataif dengan uji warna menggunakan reagen FeSO4 yang menghasilkan warna sampel keruh dan terdapat endapan. Sedangkan pada analisis kuantitif menggunakan HPLC didapatkan rata-rata kadar pada sampel rasa jeruk 1,109 mg/kg dan pada sampel nanas 0,933 mg/kg.Kata kunci : Analisis, kadar, tartrazin, pewarna, HPLC

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