Effects of Bacillus subtilis and Bacillus licheniformis on catfish in industrial aquaculture

When growing fish in high-tech breeding systems, it is exposed to many stress factors. To reduce stress, we used spore forms of microorganisms — Bacillus subtilis and Bacillus licheniformis, possessing probiotic properties. Two groups were formed, a control group and an experimental group that received probiotic microorganisms with feed. The aim of the study was to evaluate the effectiveness of the use of microorganisms — Bacillus subtilis and Bacillus licheniformis to reduce stress in fishes. For this indicator points were selected — stress hormone cortisol and antioxidant defense enzymes. The research results showed that stress level assessed by cortisol in fishes fed with probiotic microorganisms is 2.8 times lower than in the control group. The use of micronucleus test to assess cytogenetic damage showed that against the probiotic microbiota background, the number of cells with fragmented nuclei decreases by 5.7 times. Antioxidant defense enzyme activity when using Bacillus subtilis and Bacillus licheniformis increased, indicating activation of the defense system against oxidative stress. The obtained results demonstrated the high efficiency of the use of spore forms Bacillus subtilis and Bacillus licheniformis when growing catfish in high-tech fish farming systems. The Russian Foundation for Basic Research has supported our study with the grant No. 18-016-00127.

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Probiotics in the diets of laying quails

Kormlenie sel'skohozjajstvennyh zhivotnyh i kormoproizvodstvo (Feeding of agricultural animals and feed production) ◽

10.33920/sel-05-2001-06 ◽

2020 ◽

pp. 60-66

Author(s):

O. Merzlyakova ◽

V. Rogachyev ◽

V. Chegodaev

Keyword(s):

Bacillus Subtilis ◽

Bacillus Licheniformis ◽

Egg Production ◽

Positive Impact ◽

Hematological Parameters ◽

Economic Effect ◽

Egg Laying ◽

Control Group ◽

Egg Mass ◽

The Cost

The efficiency of introducing probiotics based on strains of Bacillus subtilis, Bacillus licheniformis and their consortium in the amount of 150 g/t of feed into the diets of laying quails has been studied. The experiment lasting 182 days has been carried out on four groups of quails with 30 heads in each. The quails have been housed in the broiler battery in compliance with the required microclimate conditions. Quails of all groups have been received the main diet (compound feed) developed taking into account their age and physiological characteristics. The quails of the 1st, 2nd and 3rd experimental groups in addition to the main diet received probiotics (150 g/t compound feed) based on strains Bacillus subtilis, Bacillus licheniformis and their consortium, respectively. It has been found that feeding the laying quails of the consortium of strains Bacillus subtilis and Bacillus licheniformis had the most significant positive impact on their productive performance, it allowed to increase egg production by 7,81 %, egg laying intensity by 5,0 %, egg mass yield by 9,77 %, while reducing feed expenditures for 10 eggs by 13,35 %. The yield of hatching eggs has been increased by 7,03 %, hatchability of chickens from laid and fertilized eggs by 8,33 and 8,35 %, brooding waste decreased by 21,74 %. Hematological parameters of quails during the whole experiment were within the physiological norm. The economic effect calculated on the basis of data on the cost of compound feed, probiotics and the cost of sold eggs of quail laying was 14,56 % in the 3rd experimental group (in relation to the control group).

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Effect of BioPlus YC Probiotic Supplementation on Gut Microbiota, Production Performance, Carcass and Meat Quality of Pigs

Animals ◽

10.3390/ani11061581 ◽

2021 ◽

Vol 11 (6) ◽

pp. 1581

Author(s):

Artur Rybarczyk ◽

Elżbieta Bogusławska-Wąs ◽

Alicja Dłubała

Keyword(s):

Bacillus Subtilis ◽

Meat Quality ◽

Bacillus Licheniformis ◽

Average Daily Gain ◽

Production Performance ◽

Probiotic Bacteria ◽

Control Group ◽

Production Traits ◽

Feed Conversion

The objective of the study was to determine the effects of probiotic bacteria Bacillus licheniformis and Bacillus subtilis on microbiological properties of feed mixtures and on the digestive tract content as applicable to production traits and carcass characteristics of fatteners. The experiment was performed on 83,838 fatteners from four successive (insertions) productions in two groups. From the seventy eighth day of age till marketing to the slaughter plant, the pigs were supplied with BioPlus YC probiotic (Chr. Hansen) in the amount of 400 g/t. The preparation contained a complex of probiotic bacteria Bacillus licheniformis DSM 5749, and Bacillus subtilis DSM 5750 spores in a 1:1 ratio. From the fourth insertion, after reaching a body weight of approximately 112 kg, 60 fatteners were selected from each group to measure carcass quality and half of them for meat quality evaluation. Moreover, microbiological analyses in feed and colon were performed. The study showed that BioPlus YC probiotics supplementation resulted in a significantly higher count of B. subtilis and B. licheniformis in the feed, a higher count of B. subtilis, B. licheniformis and LAB, as well as a lower count of Enterobacteriaceae, Enterococcus, Clostridium and Bacillus sp. in the mucosa and in the colorectal content of the test pigs. Our work has shown that supplementation with the BioPlus YC probiotic had a positive effect on the production traits of pigs mainly by reducing mortality (2.83%, p = 0.010), lowering feed conversion ratio—FCR (2.59 kg/kg, p = 0.013), better average daily gain—ADG (0.95 kg/day, p = 0.002) and shorter fattening period (77.25 days, p = 0.019) when compared to the control group (4.19%; 2.79 kg/kg; 0.89 kg/day; 92.8 days, respectively). The addition of the specific Bacillus bacteria did not influence carcass and meat characteristics of the test fatteners.

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Effect of probiotic strains of Bacillus subtilis on the growth parameters of broiler chickens and caecal microbiota

E3S Web of Conferences ◽

10.1051/e3sconf/202022202054 ◽

2020 ◽

Vol 222 ◽

pp. 02054

Author(s):

Ayslu Mardanova ◽

Anastasia Nikolaeva ◽

Guzel Lutfullina ◽

Aygul Ganeeva ◽

Margarita Sharipova

Keyword(s):

Bacillus Subtilis ◽

Broiler Chickens ◽

Pathogenic Bacteria ◽

Growth Parameters ◽

Live Weight ◽

Control Group ◽

Probiotic Properties ◽

Probiotic Strains ◽

Opportunistic Pathogenic ◽

Caecal Microbiota

In this paper, the probiotic properties of Bacillus subtilis GM2 and GM5 strains were studied. It is shown that the use of probiotic additives based on the spores of these bacteria leads to an increase in the live weight gain of broiler chickens by 4.16% and 10.76% relative to the control. Metagenomic analysis showed that representatives of the phylum Firmicutes (54.55%) and Bacteroidetes (30.45%), mainly represented by the families Ruminococcacea and Bacteroidaceae, predominate in the caecal microbiota of broiler chickens on day 42. It was found that a probiotic based on the B. subtilis GM5 strain leads to an increase in the proportion of Firmicutes in caecum by 27% and a decrease in Bacteroidetes by 19%. There was also a significant decrease in the number of representatives of opportunistic pathogenic bacteria of the Enterobacteriaceae family relative to the control group.

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The use of probiotics based on strains of the genus Bacillus in quail feeding

Siberian Herald of Agricultural Science ◽

10.26898/0370-8799-2019-2-9 ◽

2019 ◽

Vol 49 (2) ◽

pp. 70-76

Author(s):

O. G. Merzlyakova ◽

V. A. Rogachev

Keyword(s):

Bacillus Subtilis ◽

Weight Gain ◽

Survival Rate ◽

Bacillus Licheniformis ◽

Control Diet ◽

Live Weight ◽

Growth Period ◽

Feed Consumption ◽

Control Group ◽

Live Weight Gain

The use of trial specimens of probiotics based on strains of Bacillus subtilis, Bacillus licheniformis and their consortium was experimentally proved to be effective for quail feeding during their growth. The dynamics of live weight gain of quails was monitored. The experiment lasted for 60 days and was carried out in compliance with the standard methods. Quails that were studied were of the Japanese breed formed in four groups of 80 heads in each: one control group and three experimental ones at the age of one day. Quails were kept in a battery cage with all the required environment conditions met. All the groups were fed on the main (control) diet, developed taking into account the age and physiological characteristics of quails. However, birds of experimental groups 1-3 were additionally fed on probiotics based on strains Bacillus subtilis, Bacillus licheniformis and their consortium in the amount of 150 g/t of feed, respectively. The effect of new strains of probiotics and their consortium was tested during the study by the following parameters: the survival rate of birds, intensity of chicken growth, meat productivity and hematological indicators. The optimum variant of probiotics introduction into the diet of quails during their growth period was determined. The recommendations for the preparation of compound feeds with the use of probiotic supplements were developed. The chemical composition of quail feed and meat was studied in the biological laboratory by conventional methods of zootechnical analysis. The introduction of the consortium of strains Bacillus subtilis and Bacillus licheniformis in the amount of 150 g/t led to an increase in the survival rate of quails by 4.0%. The average daily live weight gain increased by 2.57%, feed consumption per one unit of produce decreased by 6.48%. When feeding quails on the consortium of probiotic strains Bacillus subtilis and Bacillus licheniformis in this rate, the mass of the eviscerated bird carcass increased by 3.61%, the protein content in the meat and its biological value increased by 1.06 and 11.11%, respectively. The hematological characteristics of the chickens remained within the physiological norm.

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The use of microbiological fertilizers in planting early potatoes in the Belgorod region

Kartofel` i ovoshi ◽

10.25630/pav.2021.36.43.005 ◽

2021 ◽

pp. 30-33

Author(s):

И.Н. Гаспарян ◽

А.Г. Левшин ◽

М.Е. Дыйканова ◽

Н.Ф. Денискина ◽

С.И. Смуров

Keyword(s):

Bacillus Subtilis ◽

Bacillus Licheniformis ◽

Bacillus Megaterium ◽

Wide Spectrum ◽

Azotobacter Chroococcum ◽

Stress Factors ◽

Early Potato ◽

Negative Effects ◽

Potato Varieties ◽

Working Solution

Экологически безопасная продукция пользуется большим спросом. Для ее получения можно использовать микробиологические удобрения. Использование микробиологических удобрений способствует снижению негативных последствий изменения климата и повышает устойчивость растений картофеля к стрессовым факторам. Цель работы: изучить микробиологическое удобрение Славол, обладающее широким спектром действия, на ранних сортах картофеля и выявить его влияние на продуктивность этой культуры. Славол содержит в своем составе шесть компонентов микроорганизмов (Derxia spp., Azotobacter chroococcum, Azotobacter vinelandi, Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium). Работа была выполнена в 2020–2021 годах на участке научно-производственной лаборатории по изучению систем земледелия ФГБОУ ВО Белгородский ГАУ. Почва опытного участка – чернозем выщелоченный, среднемощный, среднесуглинистый на лессовидном суглинке. В опытах были использованы следующие сорта: Удача, Брянский деликатес, Ред Скарлетт, Жуковский ранний, Метеор, Ривьера. Варианты опыта: 1) контроль и 2) применение микробиологического удобрения Славол, которое включало обработку клубней перед посадкой (10 мл/л воды, замачивание на 3–4 часа) и в период ухода дважды в виде некорневой подкормки (10 мл/10 л воды, расход рабочего раствора – 1,5 л/10 м2), первая обработка – через 10–15 дней после всходов, вторая – в период бутонизации. Повторность опытов трехкратная. Варианты в опыте были размещены рендомизированным методом. Площадь одной опытной делянки – 25 м2. Схема посадки – 70×35 см. Установлено, что микробиологическое удобрение Славол обладает широким спектром действия и влияет на рост и развитие растений: повысилось количество стеблей (в среднем по сортам на 32,6%), увеличилась высота растений (на 10,8%) и общая площадь листового аппарата (на 32,9%). В посадках картофеля на 72,2% снизилось развитие альтернариоза. Комплексное влияние препарата сказа Environmentally friendly products are in great demand. Microbiological fertilizers can be used to obtain it. The use of microbiological fertilizers helps to reduce the negative effects of climate change and increases the resistance of potato plants to stress factors. The purpose of the work: to study the microbiological fertilizer Slavol, which has a wide spectrum of action, on early potato varieties, and to identify its effect on the productivity of this crop. Slavol contains six components of microorganisms (Derxia spp., Azotobacter chroococcum, Azotobacter vinelandi, Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium). The work was carried out in 2020–2021 at the site of the scientific and production laboratory for the study of agricultural systems of the Belgorod State Agrarian University. The soil of the experimental site is leached chernozem, medium-thick, medium-loamy on loess-like loam. The following varieties were used in the experiments: Udacha, Bryansk delikates, Red Scarlett, Zhukovsky ranniy, Meteor, Riviera. Experience options: 1) control and 2) the use of microbiological fertilizer Slavol, which included the treatment of tubers before planting (10 ml/l of water, soaking for 3–4 hours) and during the care period twice in the form of foliar top dressing (10 ml/10 l of water, the consumption of the working solution of 1.5 l/10 m2) the first treatment 10–15 days after germination, the second – during budding. The repetition of experiments is threefold. The variants in the experiment were placed by a randomized method. The area of one experimental plot is 25 m2. The planting scheme is 70×35 cm. It is established that the microbiological fertilizer Slavol has a wide spectrum of action and affects the growth and development of plants: the number of stems has increased (on average for varieties by 32.6%), the height of plants has increased (by 10.8%) and the total area of the leaf apparatus (by 32.9%). In potato plantings, the development of alternariosis decreased by 72.2%. The complex effect of the drug affected the yield of early potato varieties, which increased by an average of 12.3% in the Belgorod region.

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Curcumin reduces malondialdehyde and improves antioxidants in humans with diseased conditions: a comprehensive meta-analysis of randomized controlled trials

BioMedicine ◽

10.1051/bmdcn/2019090423 ◽

2019 ◽

Vol 9 (4) ◽

pp. 23 ◽

Cited By ~ 3

Author(s):

Mohammad Alizadeh ◽

Sorayya Kheirouri

Keyword(s):

Oxidative Stress ◽

Randomized Controlled Trials ◽

Antioxidant Defense ◽

Human Subjects ◽

Meta Analysis ◽

Research Question ◽

Control Group ◽

Controlled Trials ◽

Randomized Controlled ◽

Antioxidant Defense Enzymes

Objective: This systematic review and meta-analysis was conducted to collate the effects of curcumin on MDA and antioxidant markers in individuals with diseased conditions. In this study the research question was “does curcumin supplementation improves oxidative stress and antioxidant defense enzymes in human subjects compared to a group without curcumin supplementation? Methods: This research included randomized controlled trials published in English in any year, in which intervention with curcumin was compared to either placebo, or standard of care or no intervention. Pubmed, Embase, Cochrane Central, Scopus and Google Scholar were searched. Meta-analysis was performed using RevMan (version 5.3), with standardized mean differences (SMD) and random-effects models. Results: One hundred twenty-seven titles and abstracts were identified which 17 articles were included for final analysis. The number of participants ranged from 22 to 160 across the included studies. The duration of intervention, dose of curcumin and location of outcomes measurements varied across the studies. Curcumin significantly reduced MDA [SMD −0.46 (95% CI: −0.68 to −0.25)] and increased superoxide dismutase (SOD) [0.82 (0.27 to 1.38)], catalase [10.26 (0.92 to 19.61)], and glutathione peroxidase [8.90 (6.62 to 11.19)] when compared with control group. Subgroup analyses displayed that curcumin could significantly reduce MDA levels with or without use of piperine, however it could increase SOD level in presence of piperine. Conclusions: These findings suggest that curcumin may be used as an adjunct therapy in individuals with oxidative stress. The administration of piperine with curcumin may enhance the efficacy of curcumin on antioxidant defense system.

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The Influence of Probiotics on Reproductive Parameters of Sows and Health of Their Sucklings

Folia Veterinaria ◽

10.1515/fv-2016-0028 ◽

2016 ◽

Vol 60 (3) ◽

pp. 43-46 ◽

Cited By ~ 1

Author(s):

R. Link ◽

P. Reichel ◽

P. Kyzeková

Keyword(s):

Bacillus Subtilis ◽

Bacillus Licheniformis ◽

Lower Incidence ◽

Conception Rate ◽

Control Group ◽

Reproductive Parameters ◽

Weaned Pigs ◽

Suckling Piglets ◽

Experimental Group

Abstract Thirtytwo sows were included in the trial. They were divided into the experimental group (n = 16) and a control group (n = 16). The experimental group received 1.28 × 106 Bacillus subtilis and Bacillus licheniformis per gram of feed (400 ppm BioPlus 2B, Chr. Hansen, Denmark). The trial started 2 weeks before farrowing and lasted until weaning. No significant differences were revealed in the number of piglets born alive, stillborn or the number of weaned pigs between the two groups of sows. The wean-to-first service interval was not significantly different between the groups, but sows in the experimental group had earlier first services. The conception rate did not differ. Sows in the experimental group suffered from postpartum dysgalactia syndrome (PDS) less than sows in the control group. The suckling piglets in the experimental group of sows reached better weight on day 14 of the trial and this state persisted up to the end of the experiment. The differences in the weights of the experimental group and the control group were significant at the end of the trial (P < 0.01). The experimental piglets had significantly lower incidence of diarrhoea than those in the control group (P < 0.05).

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Anamnestic factors that shape reproductive health of women with repeated unsuccessful popygamy in vitro fertilization

HEALTH OF WOMAN ◽

10.15574/hw.2016.114.140 ◽

2016 ◽

pp. 140-143

Author(s):

N.V. Cotsabin ◽

◽

O.M. Makarchuk ◽

Keyword(s):

High Risk ◽

Risk Group ◽

Polycystic Ovary ◽

High Risk Group ◽

Stress Factors ◽

Control Group ◽

Fertilization In Vitro ◽

Infertile Women ◽

Group I

The proportion of patients with multiple unsuccessful attempts of assisted reproductive technology (ART) is about 30% of all patients treated with the use of ART. Women with history of unsuccessful ART attempts - a special category of patients who require emergency attention and a thorough examination at the stage of preparation for superovulation stimulation,the selection of embryos and endometrium preparation for embryo transfer. The objective: to distinguish high-risk group of unsuccessful attempts based on a detailed analysis of anamnestic and clinical data of infertile women with repeated unsuccessful ART attempts that requires more in-depth study of hormonal features, ovarian reserve and condition of the endometrium. Materials and methods. For better understanding of the problem of repeated unsuccessful ART attempts and сreation of efficient infertility treatment algorithms for these couples we conducted a thorough analysis of anamnestic data of three groups of infertile women (105 patients), which were distributed by age: group I – younger than 35, the II group – from 35 to 40, the III group - over 40 years. These groups of patients were compared with each other and with the control group of healthy women (30 persons). Results. Leading stress factors in the percentage three times prevailed in the group of infertile women and had a direct connection with the fact of procedure «fertilization in vitro» and chronic stressors caused by prolonged infertility. Primary infertility was observed significantly more frequent in patients younger than 35 years (p <0.05), secondary infertility - mostly in the second and third experimental groups (p <0.05). Noteworthy significant percentage of wellknown causes of infertility and idiopathic factor in all groups, and the prevalence of tubal-peritoneal factor in the second and third experimental groups, and endocrine dysfunction in the I experimental group. The most common disorder among this category of woman was polycystic ovary syndrome. Frequency of usual miscarriage among patients of I ana II groups was two times higher than in the third group (p <0.05). Among the experimental groups the leading place belongs urinary tract infection, respiratory tract diseases, pathologies of the cardiovascular system. Data of the stratified analysis show an increase likelihood of repeated unsuccessful ART attempts under the influence of constant chronic stress (odds ratio OR=2.06; 95% CI: 0.95–3.17; p<0.05). Conclusions. Among infertile patients with repeated unsuccessful ART attempts must be separated a high risk group of failures. The identity depends on the duration of infertility, female age and leading combination of factors. Key words: repeated unsuccessful ART attempts, anamnesis, infertility, high risk.

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STUDY OF THE COMBINED INFLUENCE OF SODIUM THIOPENTAL AND DELTA SLEEP-INDUCING PEPTIDE ON ANTIOXIDANT DEFENSE SYSTEM IN RATS

Токсикологический вестник ◽

10.36946/0869-7922-2016-5-49-52 ◽

2016 ◽

pp. 49-52

Author(s):

A. V. Shvetsov ◽

E. G. Batotsyrenova ◽

N. A. Dyuzhikova ◽

V. A. Kashuro ◽

N. V. Lapina ◽

...

Keyword(s):

Superoxide Dismutase ◽

Antioxidant Defense ◽

Antioxidant Defense System ◽

Time Interval ◽

Sodium Thiopental ◽

Antioxidant Defense Enzymes ◽

Delta Sleep ◽

Thiopental Coma ◽

Glucose 6 Phosphate Dehydrogenase ◽

Delta Sleep Inducing Peptide

A biochemical investigation was performed into activity of rat antioxidant defense enzymes at different time interval after administration of sodium thiopental and delta-sleep-inducing peptide (DSIP). It was shown that thiopental coma was accompanied by a decreased level of superoxide dismutase ( 6 and 24 h after exposure) and increased level of caspase-3 ( 6 h after exposure) in the rat blood plasma. A pharmacological correction with DSIP induced a decrease of the level of superoxide dismutase ( 6 and 24 h after exposure), glutathione peroxidase and glucose 6-phosphate dehydrogenase (after 6h).

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Research on the methods and means of action strength training in aerobics training

International Journal of Electrical Engineering Education ◽

10.1177/00207209211004225 ◽

2021 ◽

pp. 002072092110042

Author(s):

Xiaona Yang ◽

Yang Ji ◽

Da Ke ◽

Fang Li

Keyword(s):

Strength Training ◽

Team Member ◽

High Efficiency ◽

Colleges And Universities ◽

The Body ◽

Energy Output ◽

Control Group ◽

Experimental Sample ◽

Training Method ◽

Core Strength

Aerobics is a beautiful and rhythmic sport. In recent years, aerobics has developed rapidly, and has become one of the essential courses of physical education in Colleges and universities. But the existing aerobics courses in Colleges and universities are less involved in the training process of action strength. Lack of strength quality is a common shortcoming of Aerobics Athletes in Colleges and universities. Therefore, this paper puts forward the methods and means of action strength training in aerobics training. According to the characteristics of competitive aerobics, combined with the traditional training strategy of action strength, this paper formulates the method of action strength training suitable for College Aerobics athletes. In order to further verify the effectiveness of this method, this paper takes a university aerobics team as an experimental sample to carry out a comparative analysis of the effect of action strength training. Relevant studies have proved that core strength training has the following effects on aerobics players: 1. Helps the calisthenics team member stabilize the trunk, improve the body's control ability and balance ability. 2. It is conducive to completing difficult movements of calisthenics with high efficiency and low energy consumption. 3. Helps the aerobics team member to enhance the special strength. 4. It can effectively prevent sports injuries. 5. It can improve the direction change and displacement speed of the body. 6. It can improve the energy output of the core strength to the limbs and other muscle groups during movement, so that the movement can be completed more perfectly.According to the results of the experiment, the explosive force and endurance of the experimental group after 8 weeks of training have been significantly improved, and formed a more obvious difference with the control group. The aerobics training method developed in this paper can be divided into two stages: the initial stage and the middle stage, which can better meet the students with different physical qualities. At the end of this paper, a fast strength training method is introduced, and the characteristics of different training methods are analyzed.

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