Staphylococcus aureus Biofilm Inhibition by a Phenolic Glycoside-rich Extract of Rubus ulmifolius

Planta Medica ◽  
2011 ◽  
Vol 77 (05) ◽  
Author(s):  
CL Quave ◽  
C Compadre ◽  
H Hendrickson ◽  
K Beenken ◽  
MS Smeltzer
Keyword(s):  
Staphylococcus Aureus ◽  
Phenolic Glycoside ◽  
Biofilm Inhibition ◽  
Rubus Ulmifolius

scholarly journals Antibacterial and anti-biofilm activities of crude extracts of Lawsonia inermis against Methicillin Resistant Staphylococcus aureus

2016 ◽  
Vol 9 (6) ◽  
pp. 263 ◽  
Author(s):  
Triveni A G ◽  
Suresh Kumar Mendem ◽  
Channapa T Shivannavar ◽  
Subhaschandra M Gaddad
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Multi Drug Resistance ◽  
Lawsonia Inermis ◽  
Biofilm Inhibition ◽  
Methicillin Resistant ◽  
Crude Extracts

 Objective:The continuous rise in the prevalence of multi drug resistance pathogens globally is threatening the treatment and management of infectious diseases. Ethno medicine plays a key role in the exploration for novel bioactive compounds. The present study evaluates the antibacterial and antibiofilm activities of the crude extracts of Lawsonia inermis against clinical isolates of methicillin resistant Staphylococcus aureus (MRSA).Materials and methods: Shade dried and finely powdered leaves of the plant were extracted by maceration method using six solvents methanol, acetone, ethyl acetate, chloroform, petroleum ether and n-hexane. Antibacterial and antibiofilm activities of the extracts against MDR MRSA by agar cup diffusion and tube method respectively.Results: Methanol extract showed the highest antibacterial activity of 18mm compared to other extracts. Similarly, petroleum ether extract showed highest biofilm inhibition of 84.7%. Other solvent extracts also exhibited significant biofilm inhibition [n-Hexane-83.6%, Ethyl acetate -79.5%, Chloroform-79.2%, Acetone -77% and Methanol-77%].Conclusion: The leaf extracts of L. inermis have shown promising biofilm inhibitory activity and good antibacterial activity, which can be explored for the development of new drugs for the MDR pathogens. Keywords: Antibioticacivity, Antibiofilm activity, L.inermis, MRSA

scholarly journals Bisdemethoxycurcumin Reduces Methicillin-Resistant Staphylococcus aureus Expression of Virulence-Related Exoproteins and Inhibits the Biofilm Formation

Toxins ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 804
Author(s):  
Shu Wang ◽  
Ok-Hwa Kang ◽  
Dong-Yeul Kwon
Keyword(s):  
Staphylococcus Aureus ◽  
Biofilm Formation ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Mrna Level ◽  
Accessory Gene ◽  
Biofilm Inhibition ◽  
Methicillin Resistant ◽  
Accessory Gene Regulator ◽  
Release Assay ◽  
Necrosis Factor

Methicillin-resistant Staphylococcus aureus (MRSA) is a major pathogen of nosocomial infection, which is resistant to most antibiotics. Presently, anti-virulence therapy and anti-biofilm therapy are considered to be promising alternatives. In the current work, we investigated the influence of bisdemethoxycurcumin (BDMC) on the virulence-related exoproteins and the biofilm formation using a reference strain and clinic isolated strains. Western blotting, quantitative RT-PCR, and tumor necrosis factor (TNF) release assay were performed to assess the efficacy of BDMC in reducing the expression of Staphylococcus enterotoxin-related exoproteins (enterotoxin A, enterotoxin B) and α-toxin in MRSA. The anti-biofilm activity of BDMC was evaluated through a biofilm inhibition assay. The study suggests that sub-inhibitory concentrations of BDMC significantly inhibited the expression of sea, seb, and hla at the mRNA level in MRSA. Moreover, the expression of virulence-related exoproteins was significantly decreased by down-regulating accessory gene regulator agr, and the inhibition of biofilms formation was demonstrated by BDMC at sub-inhibitory concentrations. Consequently, the study suggests that BDMC may be a potential natural antibacterial agent to release the pressure brought by antibiotic resistance.

scholarly journals 17. Oxytetracycline Activities To Staphylococcus aureus Biofilm Inhibition Of Aceh Cattle Preputium Isolate

2019 ◽  
Vol 13 (1) ◽  
pp. 125-131
Author(s):  
Maryulia Dewi ◽  
Darmawi Darmawi ◽  
T. Zahrial Helmi ◽  
Erina Erina ◽  
Basri Gani ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Optical Density ◽  
Antimicrobial Activities ◽  
Nutrient Broth ◽  
Biofilm Inhibition ◽  
Inhibitory Capacity ◽  
Inhibitory Power

The aim of this study was to see the antimicrobial activities of oxytetracycline to the inhibition of Staphylococcus aureus biofilm of Aceh’s cattle preputium isolates. The parameters measured in this study were the percentage of antimicrobial and biofilm inhibitory power. The antimicrobial activities were tested on Nutrient Broth using U-bottom polystyrene microplate 96 wells. The Biofilm stained using 1% of crystalviolet. Then, Optical Density (OD) read at λ 595 nm. The results showed that the oxytetracycline of 10%, 20% and 30% (μg / μL) had the antimicrobial abilities of 28.60% ± 13.23%; 36.30% ± 5.37%; and 57.70% ± 2.23%. In addition, The biofilm inhibitory capacity of oxytetracycline 10%, 20% and 30% (μg / μL) were 2.20% ± 8.77%; 2.70% ± 4.17%; and 3.73% ± 14.14%. Therefore, it can be concluded that the oxytetracycline  had the ability to inhibit the biofilm of Staphylococcus aureus.

scholarly journals Biofilm Inhibition and Eradication Properties of Medicinal Plant Essential Oils against Methicillin-Resistant Staphylococcus aureus Clinical Isolates

2020 ◽  
Vol 13 (11) ◽  
pp. 369
Author(s):  
Fethi Ben Abdallah ◽  
Rihab Lagha ◽  
Ahmed Gaber
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Medicinal Plant ◽  
Essential Oils ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Clinical Isolates ◽  
Mass Spectrometry Analysis ◽  
Biofilm Inhibition ◽  
Methicillin Resistant ◽  
Plant Essential Oils

Methicillin-resistant Staphylococcus aureus is a major human pathogen that poses a high risk to patients due to the development of biofilm. Biofilms, are complex biological systems difficult to treat by conventional antibiotic therapy, which contributes to >80% of humans infections. In this report, we examined the antibacterial activity of Origanum majorana, Rosmarinus officinalis, and Thymus zygis medicinal plant essential oils against MRSA clinical isolates using disc diffusion and MIC methods. Moreover, biofilm inhibition and eradication activities of oils were evaluated by crystal violet. Gas chromatography–mass spectrometry analysis revealed variations between oils in terms of component numbers in addition to their percentages. Antibacterial activity testing showed a strong effect of these oils against MRSA isolates, and T. zygis had the highest activity succeeded by O. majorana and R. officinalis. Investigated oils demonstrated high biofilm inhibition and eradication actions, with the percentage of inhibition ranging from 10.20 to 95.91%, and the percentage of eradication ranging from 12.65 to 98.01%. O. majorana oil had the highest biofilm inhibition and eradication activities. Accordingly, oils revealed powerful antibacterial and antibiofilm activities against MRSA isolates and could be a good alternative for antibiotics substitution.

Chitooligosaccharides as Antibacterial, Antibiofilm, Antihemolytic and Anti-Virulence Agent against Staphylococcus aureus

2019 ◽  
Vol 20 (14) ◽  
pp. 1223-1233 ◽  
Author(s):  
Fazlurrahman Khan ◽  
Jang-Won Lee ◽  
Dung T.N. Pham ◽  
Young-Mog Kim
Keyword(s):  
Molecular Weight ◽  
Staphylococcus Aureus ◽  
Bactericidal Activity ◽  
Biofilm Formation ◽  
Cost Effective ◽  
Bacterial Biofilm ◽  
Water Soluble ◽  
Dilution Method ◽  
Low Molecular Weight ◽  
Biofilm Inhibition

Background: Staphylococcus aureus nosocomial infections with a high mortality rate in human and animals have been reported to associate with bacterial biofilm formation, along with the secretion of numerous virulence factors. Therefore, the inhibition of biofilm formation and attenuation of virulence determinants are considered as a promising solution to combat the spread of S. aureus infections. Modern trends in antibiofilm therapies have opted for the active agents that are biocompatible, biodegradable, non-toxic and cost-effective. Owning the aforementioned properties, chitosan, a natural N-acetylated carbohydrate biopolymer derived from chitin, has been favorably employed. Recently, the chitosan structure has been chemically modified into Chitooligosaccharides (COS) to overcome its limited solubility in water, thus widening chitosan applications in modern antibiofilm research. In the present study, we have investigated the antibacterial, antibiofilm and anti-virulence activities against S. aureus of COS of different molecular weights dissolved in neutral water. Methods: The study of bactericidal activity was performed using the micro-dilution method while the biofilm inhibition assay was performed using crystal-violet staining method and confirmed by scanning electron microscopic analysis. The inhibition of amyloid protein production was confirmed by Congo Red staining. Results: Results showed that low molecular weight COS exhibited bactericidal activity and reduced the bacterial amylogenesis, hemolytic activity as well as H2O2 resistance properties, while slightly inhibiting biofilm formation. The present study provides a new insight for further applications of the water-soluble COS as a safe and cost-effective drug for the treatment of S. aureus biofilm-associated infections. Conclusion: Reducing the molecular weight of chitosan in the form of COS has become an effective strategy to maintain chitosan biological activity while improving its water solubility. The low molecular weight COS investigated in this study have effectively performed antibacterial, antibiofilm and antivirulence properties against S. aureus.

Antibacterial and antibiofilm activity of the bacteriocin-producing strain Leuconostoc mesenteroides CHBY46 isolated from Algerian dromedary milk against Pseudomonas aeruginosa and Staphylococcus aureus biofilms

2019 ◽  
Vol 8 (4) ◽  
pp. 120-131
Author(s):  
Yahia Bellil ◽  
Zineb Benmechernene ◽  
Wassila Chahrour Bellil ◽  
Mebrouk Kihal
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
16S Rrna Gene Sequencing ◽  
Antibiofilm Activity ◽  
Rrna Gene ◽  
Biofilm Inhibition ◽  
Staph Aureus ◽  
Spoilage Bacteria ◽  
Dromedary Milk ◽  
And Staphylococcus Aureus

In order to control biofilm formation of pathogenic and spoilage bacteria in foods, some species of Leuconostoc are very important in food industries, as they increase the shelf life of foods during preservation. In this study the strain CHBY46 a bacteriocin-producing strain belonging to Leuconostoc ge-nus isolated from dromedary milk in the south of Algeria was characterized by 16S rRNA gene sequencing and MALDITOF MS mass spectrometry, tested for its antibacterial and antibiofilm activities against Pseudomonas aeruginosa and Staphylococcus aureus. The produced bacteriocin was partially puri-fied with sulfate ammonium precipitation and RP-HPLC. The strain CHBY46 was classified as Leuc. mesenteroides after molecular identification. Among the bacteria tested the pathogens Staph. aureus ATCC 29213 and Ps. aeru-ginosa ATCC 27653 were sensitive to this bacteriocin with 480 AU/ml. Antibi-ofilm activity was investigated by crystal violet assay. The bacteriocin of Leuc. mesenteroides CHBY 46 exhibited significant biofilm inhibition ; 35.58% with Ps. aeruginosa, and 42.11% with Staph. aureus. Tricine-SDS-PAGE analysis of the partially purified bacteriocin indicated a low molecular weight of approximately 3.5 kDa. Therfore, we conclude that bacteriocins from Leuco-nostoc have the potential as a therapeutic strategy against pathogen’s bio-films, which contribute, to bacterial pathogenicity and resistance toward antibiotics or being used in foods as adjunsts to contribute food safety.

scholarly journals Ellagic Acid Derivatives from Rubus ulmifolius Inhibit Staphylococcus aureus Biofilm Formation and Improve Response to Antibiotics

PLoS ONE ◽  
2012 ◽  
Vol 7 (1) ◽  
pp. e28737 ◽  
Author(s):  
Cassandra L. Quave ◽  
Miriam Estévez-Carmona ◽  
Cesar M. Compadre ◽  
Gerren Hobby ◽  
Howard Hendrickson ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Ellagic Acid ◽  
Biofilm Formation ◽  
Rubus Ulmifolius ◽  
Ellagic Acid Derivatives

scholarly journals Unsaturated Fatty Acids Control Biofilm Formation of Staphylococcus aureus and Other Gram-Positive Bacteria

Antibiotics ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 788
Author(s):  
Kamila Tomoko Yuyama ◽  
Manfred Rohde ◽  
Gabriella Molinari ◽  
Marc Stadler ◽  
Wolf-Rainer Abraham
Keyword(s):  
Fatty Acids ◽  
Staphylococcus Aureus ◽  
Linoleic Acid ◽  
Unsaturated Fatty Acids ◽  
Long Chain Fatty Acids ◽  
Gram Positive ◽  
Biofilm Inhibition ◽  
Gram Positive Bacteria ◽  
Long Chain ◽  
Chain Fatty Acids

Infections involving biofilms are difficult to treat due to increased resistances against antibiotics and the immune system. Hence, there is an urgent demand for novel drugs against biofilm infections. During our search for novel biofilm inhibitors from fungi, we isolated linoleic acid from the ascomycete Hypoxylon fragiforme which showed biofilm inhibition of several bacteria at sub-MIC concentrations. Many fatty acids possess antimicrobial activities, but their minimum inhibitory concentrations (MIC) are high and reports on biofilm interferences are scarce. We demonstrated that not only linoleic acid but several unsaturated long-chain fatty acids inhibited biofilms at sub-MIC concentrations. The antibiofilm activity exerted by long-chain fatty acids was mainly against Gram-positive bacteria, especially against Staphylococcus aureus. Micrographs of treated S. aureus biofilms revealed a reduction in the extracellular polymeric substances, pointing to a possible mode of action of fatty acids on S. aureus biofilms. The fatty acids had a strong species specificity. Poly-unsaturated fatty acids had higher activities than saturated ones, but no obvious rule could be found for the optimal length and desaturation for maximal activity. As free fatty acids are non-toxic and ubiquitous in food, they may offer a novel tool, especially in combination with antibiotics, for the control of biofilm infections.

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