Attenuation of Tissue Thrombosis and Hemorrhage by ala-TFPI Does not Account for Its Protection against E. coli

1998 ◽  
Vol 79 (05) ◽  
pp. 1048-1053 ◽  
Author(s):  
M. M. Randolph ◽  
G. L. White ◽  
S. D. Kosanke ◽  
G. Bild ◽  
C. Carr ◽  
...  
Keyword(s):  
Renal Tissue ◽  
Saline Control ◽  
Control Group ◽  
Target Tissue ◽  
E Coli ◽  
Mild Degree ◽  
Target Organs ◽  
Tissue Factor Pathway ◽  
Failure To Protect ◽  
Experimental Group

SummaryThis study was designed to determine the effect of a delayed infusion (T+120 min) of alanyl tissue factor pathway inhibitor (ala-TFPI) on the response to LD100 E. coli. We hypothesized that baboons treated with a low dose of TFPI (5 mg/kg) which did not survive would exhibit thrombosis, infarction and hemorrhage of target tissues such as that seen in untreated animals infused with LD100 E. coli. Eight baboons were infused with 5 mg/kg of ala-TFPI over a 10 h period beginning immediately after a 2 h infusion of LD100 E. coli (experimental group). Four baboons were infused with E. coli followed by a 10 h infusion of saline (control group). Of the 12 baboons, the 11 non-survivors (TFPI = 7 out of 8; controls = 4 out of 4) were evaluated for the extent of thrombosis, necrosis, hemorrhage, and congestion of target tissues and for changes in clinical chemical parameters. We expected that failure to protect would correlate with failure to inhibit thrombosis of target tissue (8). Surprisingly ala-TFPI significantly inhibited thrombosis, hemorrhage and necrosis of adrenal and renal tissues and attenuated the rise in creatinine in the 7 treated non-survivors. The lungs of these non-survivors, however, exhibited intra-alveolar fibrin and a mild degree of hemorrhage and edema. We concluded that low doses of ala-TFPI begun as late as T+120 in minutes failed to protect against the lethal effects of LD100 E. coli in spite of completely preventing thrombosis and hemorrhage in target organs, and that thrombosis, infarction and hemorrhage of adrenal and renal tissue are not part of the lethal chain of events in this IV model of E. coli sepsis.

scholarly journals Assessment of Gut Microbiota and Selected Blood Parameters in Weaned Piglets Following Supplementation with a Probiotic and Vitamin C

2020 ◽  
Vol 20 (1) ◽  
pp. 179-189
Author(s):  
Leszek Tymczyna ◽  
Beata Trawińska ◽  
Marta Kowaleczko ◽  
Anna Chmielowiec-Korzeniowska ◽  
Jerzy Lechowski
Keyword(s):  
Vitamin C ◽  
Gut Microbiota ◽  
Total Cholesterol ◽  
Hdl Cholesterol ◽  
Blood Parameters ◽  
Control Group ◽  
Weaned Piglets ◽  
E Coli ◽  
Experimental Group ◽  
Control Group Group

AbstractThe aim of the study was to assess the gut microbiota and selected haematological and biochemical blood parameters of weaned piglets following dietary supplementation with a probiotic and vitamin C. the piglets were divided into a control group (group C) and an experimental group (group E), with 30 piglets in each group. All animals received the same feed ad libitum. The animals in the control group (group C) received feed with no added probiotic and vitamin C. the piglets in the experimental group (group E) were given a supplement containing a probiotic (Bacillus cereus 1×109 CFU/kg) in the amount of 1.5 g/piglet/day and vitamin c in the amount of 300 mg/piglet/day. The supplement was administered for 28 days. The total numbers of bacteria of the family Enterobacteriaceae and of the genus Lactobacillus were determined in faeces. The erythrocyte count, haemoglobin level, haematocrit, leukocyte count, and percentages of neutrophils and lymphocytes were determined in the blood. The biochemical analysis concerned the concentration of triacylglycerols, total cholesterol, and LDL and HDL cholesterol. In group E a significant decrease (P<0.01) in the total number of Enterobacteriaceae bacteria was observed in the faeces of the piglets, accompanied by an increase in the number of lactobacilli relative to group C. E. coli was found to predominate over other microorganisms. Salmonella choleraesuis bacteria were present in the faeces of both groups before administration of the supplement, but were not found after its use. The supplement with probiotic and vitamin C caused a significant increase in the erythrocyte, haemoglobin and haematocrit levels in the blood of the piglets and a significant decrease in the concentration of triacylglycerols, total cholesterol and LDL cholesterol.

scholarly journals Експериментальне зараження поросят вірусом епідемічної діареї свиней

2017 ◽  
Vol 19 (77) ◽  
pp. 208-213
Author(s):  
D. Masiuk ◽  
A. Sosnitskiy ◽  
A. Kokarev ◽  
S. Koliada
Keyword(s):  
Real Time ◽  
The Body ◽  
Control Group ◽  
Rt Pcr ◽  
Internal Organs ◽  
Real Time System ◽  
E Coli ◽  
Neonatal Piglets ◽  
And Control ◽  
Experimental Group

There were infected neonatal piglets in the first days of their lives PED virus suspension derived from pigs previously PED patients. Diagnosis for PED in piglets donor virus PED was inserted complex method for clinical and epizootic performance and confirmed the identification PEDV by PCR-RT using the test system «EZ-RED/TGE/PDCoV MPX 1.0 Real time RT-PCR» company Tetracore (USA) Thermocyclers CFX 96 Real-Time System company BIO RAD (USA). Homogenate small intestine of pigs PEDV donor, prepared in a blender for PCR in a thick band of 18 animal carcasses, frozen at -18 °C without cryopreservation and kept 359 days. Before infecting pigs and strip defrost by RT-PCR identified the concentration of the virus genome equivalents (GE) without establishing viable virions quantitative pathogen. For Sample 20 selected analog neonatal piglets, divided them into 3 experimental groups (group 1 – 5 piglets, group 2 – 5 piglets and group 3 – 7 piglets) and one control (3 piglets). Research pigs infected per os virus-containing suspension with a concentration PEDV 1.03×106 GE/cm3. The dose for infection first group was 6 cm3 (6.18×106 GE/cm3), for the second – 5 cm3 (5,15 × 106 GE/cm3), for the third – 4 cm3 (4.12 GE×106/cm3) homogenate. The fourth group – control (not infected). All the pigs were in identical conditions that fully meet the physiological needs of the body. Of the 17 infected pigs only 2 was infected PEDV. PED was confirmed by laboratory methods. In bacteriological examination of internal organs of pigs that came out of a research experiment and control group were diagnosed colibacteriosis. In the control group was isolated from heart and intestinal non-pathogenic for white mice E. coli. From pigs 1 and 2 research groups has been allocated to white mice nonpathogenic E. coli, is set colibacteriosis; 2 experimental group found in one pig hemolytic E. coli; 3 experimental group from the internal organs of pigs in conjunction with non-pathogenic for mice intestinal former cane isolated Klesiella spp., is diagnosed with mixed infection (E. coli, Klesiella spp.). From the intestine of experimental and control pigs do not identified beneficial microflora – aerococcus, lactobacteria, bifidobacteria and cultured putrefactive anaerobic spore facultative and non spore microflora.

scholarly journals Effects of Pistacia atlantica Subsp. Kurdica on Cadmium-Induced Oxidative Damage in Rat Kidney

2020 ◽  
Vol 1 (1) ◽  
pp. 10-14
Author(s):  
Seyed Salam Kohnepoushi ◽  
Dara Dastan ◽  
Amir Nili-Ahmadabadi
Keyword(s):  
Oxidative Damage ◽  
Rat Kidney ◽  
Radical Scavenging ◽  
Renal Tissue ◽  
Positive Control ◽  
Saline Control ◽  
Control Group ◽  
Pistacia Atlantica ◽  
Serum Blood Urea Nitrogen ◽  
Group 2

Background: Pistacia atlantica kurdica has recently been shown to possess free radical scavenging ability. The current study aims to investigate the protective effect of this plant against cadmium-induced nephrotoxicity. Methods: Thirty-six rats were divided into 6 groups (6 in each), and treated as follows: group 1 received normal saline (control group), group 2 (positive control) received cadmium by drinking water (100 mg/ L/d), group 3 received 200 mg/kg of P. atlantica extract, and groups 4-6 received cadmium as well as 50, 100 and 200 mg/kg/d of P. atlantica extract (orally), respectively. After 2 weeks, oxidative damage and renal function markers were assayed by standard methods. Results: In cadmium group, a significant increase was observed in serum blood urea nitrogen (BUN) (P<0.01) and lipid peroxidation (LPO) level of renal tissue (P<0.001) and a remarkable decrease was found in total thiol molecules (TTM) of the kidney (P<0.001). Despite the decreased renal antioxidant capacity, these changes were not significant. P. atlantica extract improved the LPO, TTM, and histopathological changes in renal tissue. Conclusion: In this study, although the P. atlantica extract did not have a significant effect on cadmiuminduced renal dysfunction, it did improve the oxidative/antioxidant balance in renal tissue.

Trueperella pyogenes and Escherichia coli as an etiological factor of endometritis in cows and the susceptibility of these bacteria to selected antibiotics

2014 ◽  
Vol 17 (4) ◽  
pp. 657-664 ◽  
Author(s):  
P. Brodzki ◽  
M. Bochniarz ◽  
A. Brodzki ◽  
Z. Wrona ◽  
W. Wawron
Keyword(s):  
Escherichia Coli ◽  
Clavulanic Acid ◽  
Postpartum Period ◽  
Control Group ◽  
Inflammatory Condition ◽  
Bacterial Isolates ◽  
Inflammatory Lesions ◽  
E Coli ◽  
Susceptibility Tests ◽  
Experimental Group

AbstractThe aim of this study was to determine the percentage of participation of particular species of microorganisms, isolated from the uterus of cows with endometritis and from cows without inflammatory lesions of the uterus, in the same postpartum period. The aim of the study was also to examine how long after parturition non-treated endometritis persists. Moreover, antibiotic susceptibility tests were carried out of the bacterial isolates dominating in the uterus. Forty cows were included in the study: 20 cows with endometritis (experimental group) and 20 cows without any inflammatory condition of the uterus (control group). The material for cytological and bacteriological tests was collected on the 5th, 26th, 40thand 60thday after parturition, using an intrauterine brush adapted for cows. The total number of collected isolates was 149, including 120 isolates from the uterus of cows with endometritis and 29 isolates from the uterus of cows without endometritis. The following species of microorganisms were isolated from the material collected from cows with endometritis: T. pyogenes (49.2%), E.coli (22.5%), F. necrophorum (11.7%), Staphylococcus sp. (6.7%), B. melaninogenicus (5.8%), and Streptococcus sp. (4.1%). The participation percentage of particular species of bacteria in the material collected from the uterus of cows without endometritis was as follows: T. pyogenes (27.6%), E.coli (24.2%), Staphylococcus sp. (20.7%), Streptococcus sp. (20.7%), B. melaninogenicus (3.4%) and F. necrophorum (3.4%). The highest percentage of T. pyogenes isolates was susceptible to ceftiofur (89.6%); cefoperazone (85.1%) and amoxicillin combined with clavulanic acid (79.1%). E. coli isolates were most susceptible to amoxicillin combined with clavulanic acid (100%), cefoperazone (94.1%) and oxytetracycline (82.3%).

scholarly journals Experimental Application of Sage in Rabbit Husbandry

2008 ◽  
Vol 77 (4) ◽  
pp. 581-588 ◽  
Author(s):  
R. Szabóová ◽  
A. Lauková ◽  
Ľ. Chrastinová ◽  
M. Simonová ◽  
V. Strompfová ◽  
...  
Keyword(s):  
Inhibitory Effect ◽  
Negative Influence ◽  
Feed Consumption ◽  
Control Group ◽  
Coagulase Negative Staphylococci ◽  
E Coli ◽  
Commercial Feed ◽  
Experimental Group

Salvia spp. belongs to the Labiatae family and is characterized by antimicrobial and antiinflammatory effect. The aim of this study was to test its in vitro and in vivo inhibitory effect against bacteria as well as to find an alternative possibility to use sage in the rabbit ecosystem examining biochemical, zootechnical and inmunological indicators, compared to the commercial feed mixture Xtract. Using the sage extract in in vitro tests, its inhibitory effect was noted. Under in vivo conditions, in the experimental group with sage (EG1), reduction of Pseudomonas-like sp. (p < 0.01) and E. coli (p < 0.01) was noted after 7 days of sage application compared to the control group CG2 (with Robenidin) as well as after 21 days of sage extract application, when the reduction of coagulase-negative staphylococci (p < 0.01) was detected (in comparison with the experimental group-EG2, Xtract group). In the caecum of rabbits from EG1, higher values of lactic, acetic and butyric acids were noted. The values of propionic acid were not influenced. Biochemical indicators were not influenced; however, the values of GSH Px were lower in EG1 compared to EG2. Higher phagocytic activity (18%) was noted in EG1 than in EG2 (13%) after 21 days of additives application. The reduction of Eimeria sp. oocysts was demonstrated in EG1 (sage group) after 7 days of sage application comparing to CG2 (217 OPG to 566 OPG). The animals in both experimental groups achieved higher feed consumption and weight gain, lower mortality compared to both controls. Neither of the additives had a negative influence on the health status and growth performance of rabbits.

scholarly journals Effect of Feed Supplemented With Vitamin C on Microbial Flora of Swine Faeces and Blood Morphology

2012 ◽  
Vol 56 (2) ◽  
pp. 171-175
Author(s):  
Beata Trawińska ◽  
Jerzy Lechowski ◽  
Antoni Polonis ◽  
Marta Kowaleczko
Keyword(s):  
Vitamin C ◽  
Pathogenic Bacteria ◽  
Bacterial Species ◽  
Intestinal Flora ◽  
Bacterial Count ◽  
Control Group ◽  
Total Bacterial Count ◽  
E Coli ◽  
Bacteriological Evaluation ◽  
Experimental Group

Abstract The studies involved gilts 6 weeks pre-partum allocated into the control and experimental groups. The experimental group received vitamin C at the dose of 2.5g/100 kg b.w./day. Faeces for analyses were collected for 3- and 6- week period of vitamin C dietary inclusion. The faecal material underwent quantitative and qualitative bacteriological evaluation. The count of Enterobacteriaceae was evaluated. The qualitative examination of these bacteria was made using the micro-plate method API 20E. Feed supplemented with vitamin C contributed to a statistically significant decline of the total bacterial count of Enterobacteriaceae in faeces. The qualitative bacterial analyses showed the presence of E. coli, Providecia sp., Proteus sp., and Salmonella sp. in the control and experimental groups. Enterobacter sp. was recovered only in the control group, while Lactobacillus sp. in the experimental group. The dietary administration of vitamin C significantly decreased the number of the studied bacterial species, except Salmonella rods. No statistically significant differences in the number of most blood morphotic elements following the 6- week supply of vitamin C were found; only the granulocyte count significantly increased, while lymphocyte numbers declined. Ascorbic acid inhibited the growth of pathogenic intestinal flora and reduced the pathogenic and relatively pathogenic bacteria count in the gastrointestinal tract and notably contributed to enhanced growth of beneficial bacteria.

Erythropoietin alters the pharmacokinetics of organic anions mainly eliminated by the kidney in rats

2021 ◽  
Vol 99 (4) ◽  
pp. 368-377
Author(s):  
María Julia Severin ◽  
María Herminia Hazelhoff ◽  
Romina Paula Bulacio ◽  
María Eugenia Mamprin ◽  
Anabel Brandoni ◽  
...  
Keyword(s):  
Organic Anion ◽  
Elimination Rate ◽  
Renal Tissue ◽  
Organic Anions ◽  
Saline Control ◽  
Control Group ◽  
Anion Transporters ◽  
Male Wistar Rats ◽  
Pah Clearance ◽  
Renal Expression

Erythropoietin (EPO) is a cytokine originally used for its effects on the hematopoietic system, and is widely prescribed around the world. In the present study, the effects of EPO administration on p-aminohippurate (PAH, a prototype organic anion) pharmacokinetics and on the renal expression of PAH transporters were evaluated. Male Wistar rats were treated with EPO or saline (control group). After 42 h, PAH was administered, and plasma samples were obtained at different time points to determine PAH levels. PAH levels in renal tissue and urine were also assessed. The renal expression of PAH transporters was evaluated by Western blotting. EPO-treated rats showed an increase in PAH systemic clearance, in its elimination rate constant, and in urinary PAH levels, while PAH in renal tissue was decreased. Moreover, EPO administration increased the expression of the transporters of the organic anions evaluated. The EPO-induced increase in PAH clearance is accounted for by the increase in its renal secretion mediated by the organic anion transporters. The goal of this study is to add important information to the wide knowledge gap that exists regarding drug–drug interactions. Owing to the global use of EPO, these results are useful in terms of translation into clinical practice.

Sepsis-Induced Coagulation in the Baboon Lung Is Associated with Decreased Endothelial Tissue Factor Pathway Inhibitor.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 803-803
Author(s):  
Haiwang Tang ◽  
Lacramioara Ivanciu ◽  
Todd Hamm ◽  
Fletcher B. Taylor ◽  
Cristina Lupu ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Endothelial Cells ◽  
Tissue Factor ◽  
Tissue Factor Pathway Inhibitor ◽  
Factor Vii ◽  
Sublethal Dose ◽  
Saline Control ◽  
E Coli ◽  
Activity Measurements ◽  
Tissue Factor Pathway

Abstract Activation of tissue factor (TF)-dependent coagulation is an early event in the pathogenesis of sepsis, responsible for microvascular thrombosis and consequent organ injury. We hypothesized that sepsis-induced TF procoagulant activity may be paralleled by a decreased expression or function of its natural inhibitor, tissue factor pathway inhibitor (TFPI). To test this hypothesis we used a non-human primate sepsis model in which adult baboons were infused with live E. coli at 109 CFU/kg (sublethal dose), 1010 CFU/kg (lethal dose) or saline (control group) and the animals were sacrified after 2, 8, or 24 hours. Lung tissue was snap frozen for protein and mRNA extraction or fixed and processed for light and electron microscopy. Tissue cryosections were immunostained using double/multiple fluorescence labeling approaches for TF, TFPI, factor VII/FVIIa and fibrin, in conjunction with cell markers for endothelial cells (CD31 or von Willebrand factor), leukocytes (PSGL-1), macrophages (CD68), PMN (myeloperoxidase) and platelets (gpIIb/IIIa). Large amounts of TF were detected in leukocytes, endothelial cells and platelet-rich microthrombi, starting from 2 hours and throughout the examined period. Concomitantly, confocal and electron microscopy analysis revealed increased leukocyte infiltration, platelet aggregates and fibrin deposition in the intravascular and interstitial compartments. In addition, TF induction was documented by semiquantitative RT-PCR, ELISA, western blot and factor Xa activation assays. Whereas TFPI mRNA showed only a modest increase, tissue-associated TFPI protein was found considerably decreased, especially during the first eight hours post E. coli infusion. Moreover, TFPI inhibitory activity of lung extracts from septic animals was 6–8 fold lower comparing to controls. TF activity measurements in the presence of inhibitory anti TFPI antibodies showed that only a very small fraction of endogenous TF was inhibited by tissue-associated TFPI, suggesting that most of the active TFPI available in the vascular compartment was depleted. The decrease of TFPI inhibitory potency cannot be exclusively explained by its proteolytic degradation, as we did not find significant amounts of truncated TFPI on western blots. In conclusion, our studies demonstrate that the exposure to septic and inflammatory stimuli lead to a decrease of TFPI-dependent endothelial anticoagulant potential, simultaneous with a strong TF-dependent procoagulant response. Activation of TF-dependent coagulation pathway not adequately countered by TFPI may have important roles in the pathogenesis of sepsis-associated disseminated intravascular coagulation. Strategies aimed to restore the physiological anticoagulant function of TFPI may help preventing sepsis-induced multiple organ dysfunction syndrome and death.

scholarly journals The interaction of Gram negative bacteria and S. mansoni in mice with experimental Schistosomiasis

1980 ◽  
Vol 75 (1-2) ◽  
pp. 161-172 ◽  
Author(s):  
Heonir Rocha ◽  
Vanete S. Oliveira ◽  
Moema Magnavita G. de Oliveira
Keyword(s):  
Portal System ◽  
Saline Control ◽  
Control Group ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Sterile Saline ◽  
E Coli ◽  
Significant Difference ◽  
The Difference ◽  
Gram Negative Organisms

Animals (122 mice) were infected each with eighty cercariae of S. mansoni and subsequently challenged intravenously eight weeks later with the following gram-negative organisms. S. typhi, E. coli, Klebsiella-enterobacter species, Proteus mirabilis and Pseudomonas aeruginosa. Enumeration of bacteria in the liver, spleen and blood and S. mansoni from the portal sistem was performed from one to four weeks later in infected animals. A significant difference between infection produced by S. typhi and other gram negative organisms was observed: S. typhi persisted longer in the spleen and liver and could be recovered from S. mansoni worms up to three weeks following bacterial infection. Other gram negative bacteria disappeared from S. mansoni worms after two weeks of initial challenge. Additional animals (51 mice) infected with S. mansoni were given S. typhi, E. coli or sterile saline. After two weeks, animals were sacrificed and the recovery rate of worms from the portal system, and the mesenteric and hepatic oogram were determined. in animals infected with E. coli a significant decrease in the number of worms was observed compared to the saline control group; thirty worms were recovered in the control group compared to two worms in e. coli infected animals. In addition, the patterns of oviposition was significantly different in these latter animals suggesting complete inhibition of this process. Following S. typhi infection the difference in recovery of worms and pattern of oviposition was minimal. These findings suggest a difference in the interaction of various gram negative bacteria and S. mansoni and are consistent with the clinical observation of prolonged salmonella bacteremia in patients with schistosomiasis.

The Effects of Electromagnetic Fields with a Frequency of 2450 MHz on E. coli Bacteria

2016 ◽  
Vol 29 ◽  
pp. 68-73
Author(s):  
Ozlem Coskun ◽  
Aytul Sofu ◽  
Mesud Kahriman ◽  
Selcuk Comlekci
Keyword(s):  
Public Health ◽  
Electromagnetic Waves ◽  
Control Group ◽  
Bacterial Cells ◽  
Heating Effect ◽  
Negative Effects ◽  
The Public ◽  
E Coli ◽  
Experimental Group ◽  
Growth Of Bacteria

This study analyzes the effects of the devices that work at the frequency of 2450 MHz and that release electromagnetic waves on the growth of bacteria. E. Coli bacterial cells are used in this experiment. The bacterial cult is divided into two groups under sterilized conditions. For both groups, the temperature is held constant at 37 Co while the heating effect of the devices working at the frequency of 2450 MHz is minimized. Then, the bacterial cells in the experimental group are exposed to the electromagnetic waves released by the devices working at the frequency of 2450 MHz while the control group is incubated for 8 hours without any exposure to electromagnetic waves. At each hour, samples from the E. Coli bacteria that are exposed to electromagnetic waves and from the control group. After measuring the optical density of the samples, it is found that the electromagnetic waves released by the devices working at the frequency of 2450 MHz have negative effects on the growth of bacteria. So, the effects of the electromagnetic waves released by the devices working at the frequency of 2450 MHz on the growth of the bacteria might be thermal or stress-related. However, there is a need for further physiologic and morphologic studies on this issue. The studies on this issue should be conducted for the public health.

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