scholarly journals Експериментальне зараження поросят вірусом епідемічної діареї свиней

2017 ◽  
Vol 19 (77) ◽  
pp. 208-213
Author(s):  
D. Masiuk ◽  
A. Sosnitskiy ◽  
A. Kokarev ◽  
S. Koliada

There were infected neonatal piglets in the first days of their lives PED virus suspension derived from pigs previously PED patients. Diagnosis for PED in piglets donor virus PED was inserted complex method for clinical and epizootic performance and confirmed the identification PEDV by PCR-RT using the test system «EZ-RED/TGE/PDCoV MPX 1.0 Real time RT-PCR» company Tetracore (USA) Thermocyclers CFX 96 Real-Time System company BIO RAD (USA). Homogenate small intestine of pigs PEDV donor, prepared in a blender for PCR in a thick band of 18 animal carcasses, frozen at -18 °C without cryopreservation and kept 359 days. Before infecting pigs and strip defrost by RT-PCR identified the concentration of the virus genome equivalents (GE) without establishing viable virions quantitative pathogen. For Sample 20 selected analog neonatal piglets, divided them into 3 experimental groups (group 1 – 5 piglets, group 2 – 5 piglets and group 3 – 7 piglets) and one control (3 piglets). Research pigs infected per os virus-containing suspension with a concentration PEDV 1.03×106 GE/cm3. The dose for infection first group was 6 cm3 (6.18×106 GE/cm3), for the second – 5 cm3 (5,15 × 106 GE/cm3), for the third – 4 cm3 (4.12 GE×106/cm3) homogenate. The fourth group – control (not infected). All the pigs were in identical conditions that fully meet the physiological needs of the body. Of the 17 infected pigs only 2 was infected PEDV. PED was confirmed by laboratory methods. In bacteriological examination of internal organs of pigs that came out of a research experiment and control group were diagnosed colibacteriosis. In the control group was isolated from heart and intestinal non-pathogenic for white mice E. coli. From pigs 1 and 2 research groups has been allocated to white mice nonpathogenic E. coli, is set colibacteriosis; 2 experimental group found in one pig hemolytic E. coli; 3 experimental group from the internal organs of pigs in conjunction with non-pathogenic for mice intestinal former cane isolated Klesiella spp., is diagnosed with mixed infection (E. coli, Klesiella spp.). From the intestine of experimental and control pigs do not identified beneficial microflora – aerococcus, lactobacteria, bifidobacteria and cultured putrefactive anaerobic spore facultative and non spore microflora.

Author(s):  
S. Grikshas ◽  
N. Kulmakova ◽  
K. Spitsyna ◽  
A. Dar’in ◽  
T. Mittelshtein

Mycotoxins have been formed in feed are secondary metabolites of fungi and are quite stable substances that have teratogenic, mutagenic and carcinogenic effects. An effective way to combat mycotoxins in feed is the use of feed additives that adsorb toxins, prevent their absorption in the gastrointestinal tract of the animal and are excreted from the body. The infl uence of coconut enterosorbent Shelltic Es on fattening and meat qualities of young pigs has been studied. It has been found in the process of fattening that the highest feed digestibility was in pigs of the experimental group, in the diet of which enterosorbent has been added. The results of studies of the chemical composition and technological properties of pork have been provided. The positive eff ect of enterosorbent on precocity, absolute average daily gain of live weight and reduction of feed expenditures per 1 kg of gain has been revealed. In pigs from the experimental group the average thickness of the fat was higher and the area of the “muscle eye” was lower compared with animals of the control group, which indicates that higher rates of carcass yield have been obtained due to faster accumulation of fat tissue. The weight of internal organs of pigs indicates the intensity of metabolic processes in the body. In experimental animals the weight of the lungs was 0,1 kg lower, and the liver and heart were higher by 0,13 and 0,01 kg, respectively. Enterosorbent had no effect on the content of vitamins in the liver of pigs of the compared groups. The content of impurities of organochlorine toxicants and toxic elements in the meat and liver of animals of the experimental group was lower than that of control analogues. Therefore, the use of enterosorbent Shelltic Es promotes more active excretion of them from the body.


2019 ◽  
pp. 1-2
Author(s):  
K. Krishnaveni ◽  
M. Jothilakshmi*

INTRO: Today's generation children are growing faster in all aspects mentally,physiologically and physically too than before generation.The life span of the human being got reduced to averagely 63 age,value tells more than the words and What will be the future? Are the children are not going to see their grandfather and grandmother? These are the question which lead this study to overtaken. METHOD:The college boys from in and around Coimbatore were selected as subjects for the propose of study.The total 60 subjects were selected and they age in 18 to 21 years.Then subjects were divided into three groups,each containing 20 members.Experimental Group I given SKY yoga with Kayakalpa training and Experimental Group II were given only SKY yoga training for 5 days in week for 10 weeks and control group not given any training. RESULTS:The study shown that there was improvement in the coordination of hand- mind and ability to think measured by Shuttle cross pick-up test method to the Experimental groups I & II than the subjects in control group. The coordination of hand- mind has increased this shown that SKY yoga effects the neurological system and mental ability of the body. CONCLUSION: The result shown that SKY Yoga with Kayakalpa on college students has positively impact on the core muscles and ageing factor


2006 ◽  
Vol 34 (01) ◽  
pp. 69-76 ◽  
Author(s):  
Sang-Eun Lee ◽  
Ji-Min Seo ◽  
Jianzhu Liu ◽  
Min-Sung Hong ◽  
Young-Won Lee ◽  
...  

This study was performed to clarify the differences of the body heats between electroacupuncture analgesia (EA) and anesthesia by ketamine hydrochloride (ketamine anesthesia) in dogs. Nine clinically healthy dogs were divided into ketamine anesthesia (control: 5 heads) and EA (experimental: 4 heads) groups, respectively. The acupoints GV-5 and Bai-Hui were used. The infrared thermographic system was used to determine the body heats. The body heats was determined at areas such as the dorsocranial (DCr), dorsocaudal (DCd), ventrocranial (VCr) and ventrocaudal (VCd) regions, on pretreatment, 10, 20, 30, 50 and 90 minutes after treatments, respectively in control and experimental groups. The body heats showed decreasing tendency until 30 minutes after ketamine injection, and then showed increasing pattern until 90 minutes after at all areas investigated in the control group. However, no significant differences of the body heats in the DCr, DCd, VCr and VCd regions were found in the control group. On the other hand, the body heats showed increasing tendency until 30 minutes, and then showed decreasing pattern until 90 minutes after EA, in the experimental group. The significant difference was observed at 30 minutes in the DCr region, and also at 10, 20 and 30 minutes in the DCd regions in the experimental group ( p < 0.05). The significant differences of the body heats were detected at 20 minutes in the DCr region, at 30 minutes in the DCd region and at 30 minutes in the VCd region between the experimental and control groups ( p < 0.05). In conclusion, EA increases of the body heat in the contrary to that of ketamine anesthesia.


Author(s):  
Jinfu Liu ◽  
Feng Gao ◽  
Lugang Yuan

Background: Chinese children are poorer in sports activity as compared to foreign children and their prospects as to physical fitness are not optimistic. This study aimed to discuss the effects of diversified sports activity modules on physical fitness and mental health of preschoolers ages 4–5 years. Methods: Sixty preschoolers aged 4–5-yr-old from two kindergartens in Jiaxing of China were selected randomly during Mar-Oct 2019. These respondents were divided into the experimental group (n=30) and the control group (n=30). The experimental group implemented the diversified sports activity module for 16 wk successively, 5 d per week, and 30-45 min per day. The control group adopted conventional sports activity. The body shape, physical fitness, and mental health of the two groups were measured and compared. Results: For body shape, the height of the experimental group increased is significantly higher than that of the control group. The experimental and control groups show no significant differences in body weight. For physical fitness, the experimental group is significantly superior to the control group in testing results of standing long jump, double-feet continuous jump, balancing on one foot, tennis shot, sit-and-reach, and 10 m repeated run. For mental health, the testing results of action development, language development, and social development of the experimental group are significantly better than those of the control group. Conclusion: The diversified sports activity module is not only conducive to strengthening the physical fitness of 4–5-year-old preschoolers but also improves their mental health.


Author(s):  
Sahbanathul Missiriya ◽  
J. Suhail Hassan ◽  
Arul Anunncia

Aims: Reflex therapy relieves tension, relaxes the mind, improves the circulation of the body. In hypertension, vascular resistance increases the pressure of the blood vessels. The study was aimed to assess the pretest and post-test levels of stress and blood pressure (BP), evaluate the effectiveness of the reflex therapy among older adults with hypertension, and to associate the level of stress and BP with demographic variables. Study Design: Community-based randomized controlled trial was adopted. Place and Duration of Study: The urban area, Poonamallee, in Chennai, between October to December 2019. Methodology: Among 100 older adults, 50 participants were selected in each experimental and control group by using simple randomization. Perceived Stress Scale (PSS) and dual function BP monitor was used to assess the stress and BP respectively. Reflex therapy was provided for 6 weeks to the older adults with hypertension, who had low and moderate levels of stress in the experimental group. The different pressures such as 100%, 75%, and 50%, that was applied in the foot for 15 minutes weekly twice by reflexology stick, when the participants were in an empty stomach. The data were analyzed using descriptive and inferential statistics. Results: The mean and standard deviation value of pretest and post-test stress level was 14.84±6.02, 9.4±4.73 in experimental group, 13.32±5.3 and 13.04±5.09 in control group respectively. This proves that reflex therapy was effective in reducing stress levels which was statistically significant (P=0.001). Mean and standard deviation of systolic BP in pretest and post-test was 142.74±5.98 and 137.78±5.69; 141.11±6.18 and 142.03±6.29, and diastolic BP was 98.21±3.28; 97.31±3.15 and 97.94±3.59; 98.96±3.34 in experimental and control group respectively indicated that the reflex therapy helps to reduce the level of BP (P=0.001). Conclusion: Reflex therapy is beneficial to older adults particularly with hypertension for relieving stress and reducing systolic and diastolic BP.


2015 ◽  
Vol 8 (4) ◽  
pp. 155-158 ◽  
Author(s):  
Sun Li ◽  
Xiao-Lin Liu ◽  
Xie-Lai Zhou ◽  
Su-Jun Jiang ◽  
Hong Yuan

Abstract The toxic metal lead is a widespread environmental polutant that can adversely affect human health. However, the underlying mechanisms of lead-induced toxicity are still largely unknown. The mechanism of lead toxicity was presumed to involve cross reaction between Pb2+ and Ca2+ with calmodulin dependent systems. The aim of the present study was thus to identify differential expression of calmodulin-related genes in the spleen of lead-exposed mice. We performed microarray analysis to identify differentially expressed genes. RNAs from spleen tissue of lead exposed animals (n=6) and controls (n=6) were converted to labeled cRNA and hybridized to Illumina mouse WG-6_v2_Bead Chip. Expression profiles were analyzed using Illumina BeadStudio Application. Real-time RT-PCR was conducted to validate the microarray data. By microarray analysis 5 calmodulin-related genes (MAP2K6, CAMKK2, CXCR4, PHKA2, MYLK) were found to be differently expressed in lead exposed compared with control mice (p<0.05). The results of Real-time RT-PCR showed that MAP2K6 and CAMKK2 were up-regulated and CXCR4 was down-regulated in lead exposure, but there were no significant differences in PHKA2 and MYLK expression between the lead exposed and control group. These results show that lead exposure produced significant changes in expression of a variety of genes in the spleen and can affect calmodulin-related gene expression.


Author(s):  
Kalyani Ambule ◽  
Manjusha Mahakarkar

Background: Pregnancy brings a slew of morphological and physiological changes to the body. The gravida must not only nourish the foetus, but also adjust to a new body habit and hormonal changes. These alterations, unsurprisingly, have an impact on the musculoskeletal system, which can lead to a number of issues, such as generalized arthralgias, back pain, separation of the pelvic bones, transient osteoporosis, and tendonitis. Objectives: 1. To assess the pretest and posttest musculoskeletal pain among postpartum women in experimental and control group. 2. To assess the effectiveness of postnatal exercises related to musculoskeletal pain among postpartum women in experimental group. 3. To find association between posttest musculoskeletal pain score among postpartum women with selected demographic variables in experimental and control group. Materials and Methods: Quasi-experimental pretest posttest design is used to assess the effectiveness of postnatal exercises, among postpartum women in selected hospitals of Wardha. 80 sample size taken who fulfils the inclusion criteria, 40 postpartum women for control and 40 for experimental group with non-probability convenience sampling. Expected Results: This study is planned to assess the effectiveness of postnatal exercises to the postpartum women experiencing musculoskeletal pain syndrome. Hence, it is expected to identify the level of pain and may reduce their level of pain. The conclusion will be drawn from the results and will be published in per review journal.


2017 ◽  
Vol 19 (82) ◽  
pp. 82-87 ◽  
Author(s):  
A.I. Ivanitskaya ◽  
Ya.V. Lesyk ◽  
S.Y. Kropyvka ◽  
N.K. Hoivanovych

The purpose of the study was to investigate the effect of putting up for 58 days life of various amounts of nanosilicon citrate infusion obtained by the method using nanotechnology and metasilicate sodium, on the growth and development of the body of the rabbits of the Hyla 110 of the diurnal age. The rabbits of the control group fed without restriction a balanced granulated feed with free access to water. Animals of the experimental groups I, II and III fed feed of the control group's diet and, during the day, dispensed the citrus application, accounting for 25; 50 and 75 μg Si/kg body weight. The females of the IV and V experimental groups feed the diet of the control group and set the sodium metasilicate (Na2SiO3H2O) with water in an amount of 2.5 and 5.0 mg Si/kg body weight, respectively. The trial lasted 68 days, including a preparatory period of 10 days, an experimental one of 58 days. On the 110-th day of life, animals of all groups were killed to determine the mass indices of carcasses, internal organs and skin. Exposure of nanosilicon citrate to animals in the I, II, and III experimental groups during 58 days was characterized by an increase in body weight of 4.0; 9.5 and 8.9%, while the use of sodium metasilicate in IV and V groups increased by 3.1 and 4.2% compared with the control group. The carcass weight correlation was correlated with body weight and in animals of groups ІІ and ІІІ, respectively, was higher by 10.6 and 9.0%, while in other experimental groups it was greater in the range from 2.4 to 2.9% compared with control. Research on the development of the body of rabbits indicated a tendency to a higher level of body mass index and internal organs in animal experimental groups than in the control, although the more pronounced differences were in rabbits I–III groups, which were deposited on nanosilicon citrus application. Ribbons infusion of citrate in comparison with metasilicate of sodium and control group, to a greater extent influenced the mass of the skin and the mass of the liver, which was reflected in an increase (P < 0.05) of their mass on 110 days of life. The use of an organic compound of silicon in the amount of 50 and 75 μg Si/kg of body weight, during 58 days, contributed to the activation of metabolic processes in the skin. Perhaps higher (P < 0.05) indices of the thickness of the dermis and subcutaneous fat and the total thickness of the skin of rabbits compared with the control group were established. The conducted studies showed more pronounced positive effects on the growth and development of the body of rabbits of the organic compound of silicon in the amount of 50 and 75 μg Si/kg body weight in comparison with its inorganic compound and control group. 


IKESMA ◽  
2019 ◽  
pp. 135
Author(s):  
Rizqi Dwi Pusponegoro ◽  
Rahayu Sri Pujiati ◽  
Ragil Ismi Hartanti

Work fatigue is one of the safety and health issues that can be a risk factor for accidents at work. Based on the preliminary study conducted by researcher in the Tanjung Traditional Market, transporter workers did repetitive work which is transporting goods by climbing up and down the stairs without using any tools. That caused their jobs are at risk of fatigue. Coconut water containing electrolyte ions needed by the body when dehydration. This study aimed to analyze the effect of giving coconut water to work fatigue on transporter workers. This research use quacy experimental research method with nonequivalent control group design. In this study, there were 32 research subjects divided into 2 groups, the experimental group and control group. The experimental group that was given 600 ml of coconut water, while the control group was given in the form of mineral water as placebo. Work fatigue was measured using Reaction Timer. The results of work fatigue measurements showed that the average has decreased over 42.3 milliseconds in the experimental group and 4.9 milliseconds in the control group.


2021 ◽  
Vol 9 (2) ◽  
pp. 132
Author(s):  
Bagas Anjasmara

Introduction: Lack of physical activities or sedenetary lifestyle will affect the abilities of body, one of them is body balance, which causes several problems that are related with motor skills and one of them is increased possibililty of injury as the result of the muscle is not accustomed to hold weight more than normal activities. Improving body balance can be done by training muscles which have function to maintain the balance. Research Objectives: This study aims to identify the combination of wobble board exercise and core stability exercise is better compared to calf raise exercise and core stability exercise in improving body balance of students with love physical activities. Methods: This research used experimental methods pre and post test two group design with. The experimental group was given wobble board exercise and core stability exercise, while the control group was given calf raise exercise and core stability exercise. The number of sample in each group is 18 people and each of the group was given 3 times in a week of exercise for 6 weeks. The balance measurement in this study used standing stork test. Result: The research result is the body balance Experimental Group is increase with mean pre test score is 10,80±4,64 seconds becomes 26,51±5,41 seconds during post test, and p = 0,001. In Control Group, there is an increase body balance with mean pre test 8,39±4,51 becomes 20,54±5,18 during post test  and p = 0,001. There is a significant difference in the increase of body balance in Experimental Group and Control Group with p = 0,002 (p < 0,05). Conclusion: The combination of wobble board exercise and core stability exercise is better than the combination of calf raise exercise and core stability exercise in improving body balance of students with low physical activities. Keywords: Body Balance; Low Physical Activities; Wobble Board Exercise; Calf Raise Exercise; Core Stability Exercise


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