Histologie einer Rezidivvarikose nach Behandlung mit dem Radiofrequenzverfahren

Phlebologie ◽  
2011 ◽  
Vol 40 (04) ◽  
pp. 217-220 ◽  
Author(s):  
D. Helbig ◽  
M. Averbeck ◽  
J. Ch. Simon ◽  
T. Wetzig ◽  
M. Kendler
Keyword(s):  
Cd 31

ZusammenfassungRezidivvarizen an der saphenofemoralen Mün-dung (SFJ) nach Crossektomie und Stripping der Stammvene sind ein bekanntes Problem. Unter Rezidivvarikose werden Krampfadern verstan-den, die in einem zuvor behandelten Stromgebiet auftreten. Das Auftreten einer Rezidivvarikose der Stammvene nach Behandlung mit dem Radiofrequenzverfahren und deren his-topathologischen Muster sind jedoch unklar. Wir zeigen an einem Fallbericht das histopathologische Muster an einer exzidierten und reperfundierten Stammvene. Der 66-jährige Patient wurde 2007 mit Radiofrequenzablation (VNUS Closure-Plus Verfahren®) an der linken Stammvene (V. saphena magna) behandelt. Ein Jahr danach zeigten sich erneut Seitenastvarizen an der linken unteren Extremität. Duplexsonographisch zeigte sich die Vene an der SFJ rekanalisiert und dilatiert. Es erfolgte eine Crossektomie, Teilsaphenektomie und Seitenastexhairese. Dabei wurde der proximale Anteil der VSM histologisch beurteilt. Neben einer Hämatoxylin/Eosinund Elastica-van-Gieson-Färbung, wurden immunhistochemische Färbungen mit Antikörper gegen α-sma und CD 31 durchgeführt. Der Fall-bericht zeigt, dass im Anschluss einer Radiofrequenzablation ein multiluminaler Rekanalisierungsprozess mit ausgekleideten Endothel auftreten kann. Unklar ist jedoch, welcher Mechanismus und welche Faktoren zur Wiedereröffnung von behandelten Venen führen.

CD 31 Quantitative Immunocytochemical Assays in Breast Carcinomas:Correlation With Current Prognostic Factors

1995 ◽  
Vol 103 (4) ◽  
pp. 443-448 ◽  
Author(s):  
Colette Charpin ◽  
Benedicte Devictor ◽  
Denise Bergeret ◽  
Lucile Andrac ◽  
Joelle Boulat ◽  
...  
Keyword(s):  
Prognostic Factors ◽  
Cd 31

Markers of angiogenesis in cervical cancer: A Gynecologic Oncology Group study

2007 ◽  
Vol 25 (18_suppl) ◽  
pp. 5536-5536
Author(s):  
L. Randall-Whitis ◽  
B. J. Monk ◽  
E. S. Han ◽  
K. Darcy ◽  
R. A. Burger ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
High Risk ◽  
Cox Regression ◽  
Gynecologic Oncology ◽  
Positive Control ◽  
Rank Test ◽  
Gynecologic Oncology Group ◽  
Continuous Variables ◽  
Histologic Subtype ◽  
Cd 31

5536 Background: Extensive tumor angiogenesis has correlated with poorer progression-free and overall survival in cervical cancer; however, specific markers of angiogenesis have not been studied prospectively. Methods: Cervical cancer patients with high-risk features on radical hysterectomy were eligible for randomization to adjuvant pelvic irradiation ± radiosensitizing platinum. Following central pathology review, formalin-fixed, paraffin-embedded tumors were sectioned into 4-micron specimens. Semi-quantitative immunohistochemisty (IHC) was performed using previously validated antibodies against mutant p53 (mp53), vascular endothelial growth factor (VEGF), thrombospondin-1 (TSP-1), and endothelial markers CD 31 and CD 105. Tumoral histoscores (HS) were calculated for mp53 and VEGF using the formula: [% cells positive × (intensity +1)], with a 5% threshold for positivity and intensity ranging 1–4+ (3+ = intensity of positive control). Intensity scores (0–4+) were assigned to TSP-1 specimens referencing the positive control (3+). MVD “hotspots” were counted in a 20X high-power field. HS and MVD counts were considered as continuous variables and TSP-1 intensity as an ordinal variable. Associations between markers were determined by Pearson’s and Spearman’s correlation tests, between markers and clinico-pathologic variables by Wilcoxon rank test, and between markers and survival by Cox regression modeling. Results: One hundred seventy-six specimens were analyzed. Acquisition of mp53 and increased VEGF expression were associated with increased MVD assessed by both CD31 (p=0.08 and p=0.002, respectively) and CD105 (p=0.02 and p=0.012, respectively). Statistically significant associations between markers and high-risk pathologic factors included: low-level TSP-1 and high CD-105 counts with lymph node metastases; high VEGF scores with advanced stage, non-squamous histologic subtype, and depth of tumor invasion; and high CD 31 counts with parametrial metastases. Survival analysis is currently being performed. Conclusions: Angiogenesis occurs early in cervical carcinogenesis, and may be a rational target for biologic therapy in cervical cancer. No significant financial relationships to disclose.

scholarly journals Functional imaging and circulating biomarkers of response to regorafenib in treatment-refractory metastatic colorectal cancer patients in a prospective phase II study

Gut ◽  
2017 ◽  
Vol 67 (8) ◽  
pp. 1484-1492 ◽  
Author(s):  
Khurum Khan ◽  
Mihaela Rata ◽  
David Cunningham ◽  
Dow-Mu Koh ◽  
Nina Tunariu ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Metastatic Colorectal Cancer ◽  
Phase Ii ◽  
Unmet Need ◽  
Predictive Biomarkers ◽  
Transfer Constant ◽  
Potential Health ◽  
Economic Implications ◽  
Dce Mri ◽  
Cd 31

ObjectiveRegorafenib demonstrated efficacy in patients with metastatic colorectal cancer (mCRC). Lack of predictive biomarkers, potential toxicities and cost-effectiveness concerns highlight the unmet need for better patient selection.DesignPatients with RAS mutant mCRC with biopsiable metastases were enrolled in this phase II trial. Dynamic contrast-enhanced (DCE) MRI was acquired pretreatment and at day 15 post-treatment. Median values of volume transfer constant (Ktrans), enhancing fraction (EF) and their product KEF (summarised median values of Ktrans× EF) were generated. Circulating tumour (ct) DNA was collected monthly until progressive disease and tested for clonal RAS mutations by digital-droplet PCR. Tumour vasculature (CD-31) was scored by immunohistochemistry on 70 sequential tissue biopsies.ResultsTwenty-seven patients with paired DCE-MRI scans were analysed. Median KEF decrease was 58.2%. Of the 23 patients with outcome data, >70% drop in KEF (6/23) was associated with higher disease control rate (p=0.048) measured by RECIST V. 1.1 at 2 months, improved progression-free survival (PFS) (HR 0.16 (95% CI 0.04 to 0.72), p=0.02), 4-month PFS (66.7% vs 23.5%) and overall survival (OS) (HR 0.08 (95% CI 0.01 to 0.63), p=0.02). KEF drop correlated with CD-31 reduction in sequential tissue biopsies (p=0.04). RAS mutant clones decay in ctDNA after 8 weeks of treatment was associated with better PFS (HR 0.21 (95% CI 0.06 to 0.71), p=0.01) and OS (HR 0.28 (95% CI 0.07–1.04), p=0.06).ConclusionsCombining DCE-MRI and ctDNA predicts duration of anti-angiogenic response to regorafenib and may improve patient management with potential health/economic implications.

scholarly journals Therapeutic Effects of Acupuncture through Enhancement of Functional Angiogenesis and Granulogenesis in Rat Wound Healing

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Sang In Park ◽  
Yun-Young Sunwoo ◽  
Yu Jin Jung ◽  
Woo Chul Chang ◽  
Moon-Seo Park ◽  
...  
Keyword(s):  
Wound Healing ◽  
Collagen Type ◽  
Interleukin 1 ◽  
Treated Group ◽  
Collagen Type I ◽  
Control Group ◽  
Therapeutic Effects ◽  
Type I ◽  
Matrix Remodeling ◽  
Cd 31

Acupuncture regulates inflammation process and growth factors by increasing blood circulation in affected areas. In this study, we examined whether acupuncture has an effect on wound healing in injured rat. Rats were assigned randomly into two groups: control group and acupuncture group. Acupuncture treatment was carried out at 8 sites around the wounded area. We analyzed the wound area, inflammatory cytokines, proliferation of resident cells, and angiogenesis and induction of extracelluar matrix remodeling. At 7 days after-wounding the wound size in acupuncture-treat group was decreased more significantly compared to control group. In addition, the protein levels of proinflammatory cytokines such as tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β) were significantly decreased compared to the control at 2 and 7 days post-wounding. Also, we analyzed newly generated cells by performing immunostaining for PCNA and using several phenotype markers such as CD-31,α-SMA, and collagen type I. In acupuncture-treated group, PCNA-positive cell was increased and PCNA labeled CD-31-positive vessels,α-SMA- and collagen type I-positive fibroblastic cells, were increased compared to the control group at 7 days post-wounding. These results suggest that acupuncture may improve wound healing through decreasing pro-inflammatory response, increasing cell proliferation and angiogenesis, and inducing extracellular matrix remodeling.

scholarly journals Expression of PCNA, CD-31 and HER-2 in Serbian patients with oral squamous cell carcinoma

2019 ◽  
Vol 71 (4) ◽  
pp. 703-710
Author(s):  
Slobodan Loncarevic ◽  
Denis Brajkovic ◽  
Milka Gardasevic ◽  
Olivera Loncarevic ◽  
Nebojsa Ladjevic ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Tumor Cells ◽  
Squamous Cell ◽  
Stage Ii ◽  
Stage Iiia ◽  
Cd 31 ◽  
Oral Squamous Carcinoma ◽  
Her 2

Several studies have investigated the expression of tumor markers, including p53, HER-2, PCNA, EGFR, VEGFR CD-31 and Bcl-2 in patients with oral squamous carcinoma (OSC). This study aimed to determine the expression of proliferating cell nuclear antigen (PCNA), endothelial functions of platelet/endothelial cell adhesion molecule-1 (CD31) and human epidermal growth factor receptor-2 (HER-2) according to OSC stage. The prospective study included 62 patients diagnosed with OSC stages II and III. Surgical specimens were obtained from tumor and peritumoral tissues. We determined the pathohistological degree of tumor differentiation and the immunohistochemical expression of PCNA, CD-31 and HER-2 for each specimen. Immunohistochemical analysis of the expression of PCNA in tumor cells demonstrated poor staining of immunoreactive tumor cells in 23 patients (10 in stage II, 7 in stage IIIa and 6 in stage IIIb). Moderately expressed PCNA-immunoreactivity in the tumor cells in 17 patients (7 in stage II, 6 in stage IIIa and 4 in stage III), and extremely strong PCNA-immunoreactive staining in tumor cells of 10 patients with IIIb stage, was observed. These results suggest that PCNA expression combined with pathohistological findings could possess a prognostic value in determining the survival rates for patients with oral squamous cell carcinoma.

Effect of Rho/ROCK pathway inhibition on metastasis-free and overall survival in biomarker selected, orthotopic, patient-derived models of pancreatic cancer.

2017 ◽  
Vol 35 (15_suppl) ◽  
pp. e15759-e15759
Author(s):  
Venessa T. Chin ◽  
Adnan Nagrial ◽  
James Conway ◽  
Claire Vennin ◽  
Lorraine A. Chantrill ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Overall Survival ◽  
Liver Tissue ◽  
Cellular Proliferation ◽  
Imaging System ◽  
Normal Liver ◽  
Human Condition ◽  
Normal Liver Tissue ◽  
Saline Control ◽  
Cd 31

e15759 Background: PC is a highly lethal disease which metastasises early. The sequencing efforts of the Australian Pancreatic Cancer Genome Initiative (APGI) identified ROCK-1 as a target of interest. The Rho/ROCK pathway is essential in cellular movement, metastasis, vasculogenesis and stromal signaling. Fasudil (F) is a ROCK inhibitor which has been shown to be safe for human use. Using unique, patient derived models, we have previously shown that F plus gemcitabine (G) does not reduce cellular proliferation in vitro but reduces tumour size and improves overall survival (OS) in subcutaneous patient-derived xenografts (PDX). However, PDXs are critisised for not fully recapitulating the human condition. Here we aimed to generate a patient-derived orthotopic xenograft (O-PDX) which could be used to assess the effects of F + G on metastasis formation and OS. Methods: One patient-derived cell line (PDCL) was labled with firefly luciferase. This was injected into the pancreas of NOD/Shi- scid/IL-2Rγnull (NSG) mice to generate the O-PDX model. The O-PDX was imaged weekly with the IVIS imaging system. The O-PDXs were treated with saline control (S) (n = 8), gemcitabine (G) (n = 10) fasudil (F) (n = 6) or gemcitabine + fasudil (GF) (n = 10). Mice were culled once radiographic or clinical evidence of ascites was detected. Metastasis free (MFS) and OS were recorded. Vessel quantification was performed via CD-31 staining. Results: Metastases seen (spleen, liver, lung, ovary) on imaging were confirmed using GFP stains on immunohistochemistry. F prolonged MFS but not OS when compared with S. GF significantly prolonged MFS (33.5 versus 43 days; p = 0.0339) and OS (51.5 versus 64 days; p = 0.0035). CD-31 staining showed that treatment with F resulted in increased vascularity in the liver metastases and normal liver tissue but not in the pancreas tumour. Conclusions: The addition of Fasudil to gemcitabine improves MFS and OS in an orthotopic model of PC and is a promising new therapeutic option. The increase in vascularity of the metastases and normal liver tissue suggest that changes in vascularity are important and may result in improved delivery of gemcitabine.

scholarly journals Differential Prox-1 and CD 31 expression in mucousae, cutaneous and soft tissue vascular lesions and tumors

2005 ◽  
Vol 201 (12) ◽  
pp. 771-776 ◽  
Author(s):  
Rui M. Reis ◽  
Jorge S. Reis-Filho ◽  
Adhemar Longatto Filho ◽  
Stanislav Tomarev ◽  
Paula Silva ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Vascular Lesions ◽  
Cd 31

scholarly journals A quantitative NLTE analysis of visual and ultraviolet spectra of four helium-rich subdwarf O stars

2018 ◽  
Vol 620 ◽  
pp. A36 ◽  
Author(s):  
M. Schindewolf ◽  
P. Németh ◽  
U. Heber ◽  
T. Battich ◽  
M. M. Miller Bertolami ◽  
...  
Keyword(s):  
Late Phase ◽  
Equal Mass ◽  
Spectral Energy ◽  
Low Oxygen ◽  
Abundance Pattern ◽  
Detailed Model ◽  
Ultraviolet Spectra ◽  
Cd 31 ◽  
Abundance Patterns ◽  
Far Ultraviolet

Aims. Hot subdwarf stars represent a poorly understood late phase of stellar evolution. While binary evolution plays an important role for the formation of B-type subdwarfs (sdB), the origin of the helium dominated subclass of O-type subdwarfs (He-sdO) is still unknown. We search for chemical signatures of their genesis by means of quantitative spectral analyses of high-quality visual and ultraviolet spectra. Methods. Four prototypical He-sdO stars, one belonging to the nitrogen-rich and three to the C-rich subclass, were selected for which archival far-ultraviolet spectra from the FUSE satellite as well as high-resolution visual and UVA spectra taken with the ESO-UVES/FEROS spectrographs are available. Using T LUSTY200/S YNSPEC49 to compute line blanketed-non-local thermodynamic equilibrium (NLTE) model atmospheres and synthetic spectra, atmospheric parameters and the abundances patterns have been derived. The final models included H, He, C, N, O, Ne, Mg, Al, Si, P, S, Fe, and Ni represented by the most detailed model atoms available. Because of the enrichment of either nitrogen or carbon, it turned out, that models including these elements at the appropriate high abundance provide sufficiently accurate approximations to the temperature stratification of full models. Results. No indications for binarity were found, neither radial velocity variations nor photometric evidence for the presence of a companion could be detected. All stars have helium-dominated atmospheres almost free of hydrogen and temperatures between 42 000 K and 47 000 K while their surface gravities lie between log g = 5.4 and 5.7. The abundance pattern of CD–31°4800 displays the signatures of CNO burning, while heavier elements are subsolar by about 0.4 dex, except for Ne and Si which are close to solar. The abundance patterns of the C-rich He-sdOs are more complex. A slightly subsolar metallicity is accompanied by N-enrichment and O-deficiency, less pronounced than in CD–31°4800. Neon is mildly to strongly enriched, up to a factor of ten with respect to the sun in LS IV +10° 9. The nickel-to-iron ratio is significantly super-solar. Using spectral energy distributions and Gaia parallaxes the masses of the stars were determined. They are found to scatter around the canonical mass for the core helium flash, although the uncertainties are large. Conclusions. The abundance pattern observed for CD–31°4800 is consistent with predictions of models for slow (cold) mergers of pairs of equal mass helium WDs except for the low oxygen abundance observed. Models for composite mergers were considered for the C-rich stars, but predict abundance pattern dissimilar to those determined. [CW83] 0904−02, though, may be a candidate for a composite He-WD merger, as it rotates and appears to be more massive than the other program stars. New evolutionary models for the hot flasher scenario predict abundance patterns similar to those determined for the C-rich stars. Hence, C-rich He-sdO may well result from late He flashes with deep-mixing episodes.

scholarly journals Chemical fingerprints of He-sdO stars

2018 ◽  
Vol 27 (1) ◽  
pp. 27-34 ◽  
Author(s):  
Markus Schindewolf ◽  
Peter Németh ◽  
Ulrich Heber ◽  
Tiara Battich ◽  
Marcelo M. Miller Bertolami ◽  
...  
Keyword(s):  
Spectroscopic Analysis ◽  
Atmospheric Parameters ◽  
Intermediate Mass ◽  
Solar Abundance ◽  
Hydrogen Burning ◽  
Cd 31 ◽  
Chemical Fingerprints ◽  
Synthetic Spectra ◽  
Cno Cycle ◽  
Formation History

Abstract The chemical composition of helium-rich hot subluminous O stars plays an important role to understand and model their formation history. We present a spectroscopic analysis of four He-sdO stars,CD-31° 4800, [CW83] 0904- 02, LSS 1274 and LS IV +10° 9. The analysis is based on archival optical and UV high-resolution spectra. We used Tlusty200/Synspec48 to compute line blanketed non-LTE model atmospheres and their corresponding synthetic spectra and derive the atmospheric parameters as well as the abundances of the most prominent elements. All stars have helium-dominated atmospheres with hardly any hydrogen and temperatures between 42000 K and 47000 K while their surface gravity spans between log g = 5.4 and 5.7. CD-31° 4800 shows an enrichment of nitrogen and the characteristic pattern of hydrogen burning via the CNO-cycle, while the rest of the elements have about the solar abundance. This points to the slow merger of two helium white dwarfs as the most likely origin for this system. The other three stars are enriched in carbon, nitrogen and neon while their intermediate mass element’s abundance scatters around the solar value. They were possibly formed in the deep mixing late hot flasher scenario.

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