Supplementation with N-carbamylglutamate and vitamin C: improving gestation and lactation outcomes in sows under heat stress

2018 ◽  
Vol 58 (10) ◽  
pp. 1854 ◽  
Author(s):  
Tao Feng ◽  
Jiahua Bai ◽  
Xiaoling Xu ◽  
Yong Guo ◽  
Zheng Huang ◽  
...  
Keyword(s):  
Heat Stress ◽  
Vitamin C ◽  
Respiration Rate ◽  
Reproductive Performance ◽  
Immunoglobulin G ◽  
Feed Intake ◽  
Control Diet ◽  
Dietary Supplementation ◽  
Maternal Circulation ◽  
Cortisol Levels

The objective of the study was to determine the effects of dietary supplementation with N-carbamylglutamate (NCG) or NCG in combination with vitamin C on the gestation and lactation of sows under heat stress during summer. The experiment lasted 51 days from the last month of gestation to weaning (Day 21 of lactation) and involved 100 Yorkshire sows, divided into four groups, fed as follows: (1) Control diet, (2) Control diet plus 0.05% NCG, (3) Control diet plus 0.05% vitamin C, and (4) Control diet plus 0.05% NCG and 0.05% vitamin C. The reproductive performances and partial serum indicators of sows were measured. The results indicated that either NCG or vitamin C administration can ameliorate heat stress and improve the reproductive performance of sows during summer. When administering NCG and vitamin C, litter weights (16.12/13.86 kg, P < 0.05) and average weights of living piglets (1.56/1.34 kg, P < 0.05), rather than numbers born alive (10.47/10.30 head, P > 0.05), were greatly increased at farrowing, and subsequently litter weights (59.69/48.61 kg, P < 0.05) and average weights of living piglets (6.00/5.16 kg, P < 0.05) at weaning were greatly increased in comparison with Controls. The combinational additives can decrease malonaldehyde and cortisol levels, and enhance immunoglobulin G level in the maternal circulation, as well as decrease the respiration rate and increase feed intake of sows. The results demonstrate that NCG in combination with vitamin C can enhance piglet weight rather than number during gestation and lactation and has important implications for anti-heat stress during summer.

scholarly journals Dietary phytogenic mixture for broilers reared under thermoneutral and heat stress conditions

2020 ◽  
Vol 23 (2) ◽  
pp. 101-116
Author(s):  
Saracila Mihaela ◽  
Panaite Tatiana Dumitra ◽  
Papuc Camelia Puia ◽  
Predescu Corina Nicoleta ◽  
Untea Arabela
Keyword(s):  
Oxidative Stress ◽  
Heat Stress ◽  
Control Diet ◽  
Dietary Supplementation ◽  
Experimental Diet ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Feeding Trials ◽  
And Oxidative Stress ◽  
Protein And Lipid Oxidation

Abstract During two feeding trials, the effect of dietary phytogenic mixture on the performance and oxidative stress biomarkers in the liver of broilers reared under thermoneutral conditions (TN) and heat stress (HS) was studied. A number of 60 Cobb 500 chicks/trial were sheltered in environmentally-controlled digestibility cages. On the 14 days of age, the chicks were weighted and assigned to four groups (2 groups/trial with 30 chicks/group). In the first trial, two groups (C-TN and PM-TN) were kept in thermoneutral conditions. In the second trial, other two groups were kept (C-HS and PM-HS) in heat stress (32 ±1 °C). The structure of diets was the same in both experiments. Compared with the control diet (C), the experimental diet (PM) contained the addition of 1% phytogenic mixture (bilberry leaves, peppermint leaves, fennel leaves and sea buckthorn meal). Irrespective of temperature conditions, dietary PM did not affect broiler’s performance. The dietary supplementation of PM delayed protein and lipid oxidation in the liver tissue of broilers in both trials by increasing the hepatic catalase, glutathione and superoxide dismutase activity.

scholarly journals Effects of purslane (<i>Portulaca oleracea</i> L.) powder on growth performance, blood indices, and antioxidant status in broiler chickens with triiodothyronine-induced ascites

2017 ◽  
Vol 60 (3) ◽  
pp. 315-325 ◽  
Author(s):  
Mahmood Habibian ◽  
Ghorbanali Sadeghi ◽  
Ahmad Karimi
Keyword(s):  
Body Weight ◽  
Feed Intake ◽  
Alanine Aminotransferase ◽  
Broiler Chickens ◽  
Antioxidant Status ◽  
Production Efficiency ◽  
Control Diet ◽  
Dietary Supplementation ◽  
Efficiency Index ◽  
Blood Indices

Abstract. This study was carried out to evaluate the effects of dietary supplementation of purslane powder (PP) on performance, blood indices, and antioxidant status in broilers with triiodothyronine (T3)-induced ascites. In total, 240 one-day-old male broiler chicks (Ross 308) were randomly assigned to four treatments, with four replicates per treatment and 15 birds per replicate. The experimental diets included (i) a control diet, (ii) a control diet plus 1.5 mg kg−1 of T3 (T3 diet), (iii) a T3 diet with the addition of 1.5 g kg−1 of PP, and (iv) a T3 diet with the addition of 3 g kg−1 of PP. Feed intake and body weight were measured at 10, 24, 39, and 49 days of experiment. Blood and liver samples were collected from two birds in each replicate at 24 and 49 days of experiment. The T3-treated birds had higher (P < 0. 05) right ventricle to total ventricle (RV ∕ TV) ratio and mortality due to ascites compared with the control. In addition, during the entire experimental period (0 to 49 days of experiment) the T3-treated birds had lower (P < 0. 05) feed intake, body weight gain, and production efficiency index and higher (P < 0. 05) feed conversion ratio compared with the control. Dietary supplementation of PP reduced (P < 0. 05) mortality due to ascites and RV ∕ TV ratio, while the production efficiency index was increased (P < 0. 05) by the addition of PP to the diet. The T3-treated birds had higher (P < 0. 05) red blood cell counts, hematocrit percentage, and hemoglobin concentration compared with the control at 24 and 49 days of experiment. Dietary supplementation of PP substantially alleviated (P < 0. 05) the negative effects of T3 on hematocrit and hemoglobin values at both 24 and 49 days of experiment and on red blood cells counts at 49 days of experiment. The T3 birds showed an increase (P < 0. 05) in activities of lactate dehydrogenase, alanine aminotransferase, and aspartate aminotransferase at 49 days of experiment. However, the detrimental effect of T3 on alanine aminotransferase activity was attenuated (P < 0. 05) by dietary supplementation of PP. The plasma and liver activities of superoxide dismutase, catalase, and glutathione peroxidase were lower (P < 0. 05) in T3-treated birds compared with the control at 24 and 49 days of experiment, whereas malondialdehyde concentrations were elevated (P < 0. 05) by dietary T3 administration. Dietary supplementation of PP, especially at 3 g kg−1, increased (P < 0. 05) the plasma and liver activities of antioxidant enzymes, and reduced (P < 0. 05) the plasma and liver concentrations of malondialdehyde near to the control levels. It is concluded that the supplementation of 3 g kg−1 of PP in diet improves oxidative status and reduces ascites incidence in broiler chickens without impairing their growth performance.

scholarly journals Dietary supplementation of dried plum: a novel strategy to mitigate heat stress in broiler chickens

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Sanjeev Wasti ◽  
Nirvay Sah ◽  
Amit K. Singh ◽  
Chin N. Lee ◽  
Rajesh Jha ◽  
...  
Keyword(s):  
Body Weight ◽  
Heat Stress ◽  
Feed Intake ◽  
Broiler Chickens ◽  
Average Daily Gain ◽  
Health Benefits ◽  
Dietary Supplementation ◽  
Broiler Chicks ◽  
Gut Health ◽  
Final Body Weight

Abstract Background Heat stress is a significant problem in the poultry industry, causing a severe economic loss due to its detrimental effects on chickens’ health and performance. Dried plum (DP) is a good source of minerals, vitamins, antioxidants, and phenolic compounds. Studies have suggested that DP has several health benefits, such as maintaining the body’s redox system, immune status, and calcium hemostasis. Based on the health benefits of DP, we hypothesized that the dietary supplementation of DP would alleviate the detrimental effects of heat stress on broiler chickens. Results To test the hypothesis, day-old broiler chicks (n = 72) were randomly allocated to three treatment groups (n = 24/group): no heat stress (NHS), heat stress (HS), and heat stress with dried plum (HS + DP), and reared under standard conditions. The inclusion of 2.5% DP in the feed of the HS + DP group was made during the treatment period, while birds in other groups were provided with a standard finisher diet. After 21 days, birds in the HS and HS + DP groups were exposed to cyclic heat stress conditions (33 °C for 8 h during daytime) for 3 weeks, while those in the NHS group were reared under normal conditions (22–24 °C). Weekly body weight and feed intake were recorded to calculate the average daily gain (ADG), average daily feed intake (ADFI), and feed conversion ratio (FCR). Heat stress significantly decreased the final body weight, ADG, ADFI, and increased FCR compared to the NHS group, whereas dietary supplementation of DP significantly improved these growth performance parameters compared to the HS group. Furthermore, supplementation of DP significantly increased the expression of heat shock protein-related genes (HSF1, HSF3, HSP70, and HSP90), antioxidant-related genes (SOD1, SOD2, GPX1, GPX3, PRDX1, and TXN), tight junction-related genes (CLDN1, and OCLN), and immune-related genes (IL4, MUC2) in the ileum as compared to the HS group. The microbiota analysis showed significant enrichment of Bacillales, Christensenellaceae, Bacillaceae, Peptostreptococcaceae, and Anaerotruncus in heat-stressed birds supplemented with DP as compared to the HS group. Further, DP supplementation also significantly increased the concentration of acetate, propionate, and total VFA in the cecal digesta of the HS + DP group as compared to the HS group. Conclusion These findings suggest that DP supplementation effectively improved the growth performances and gut health parameters in the heat-stressed birds. Thus, dried plum can be a potential feed supplement to mitigate heat stress in broiler chickens.

scholarly journals Effects of chromium supplementation on physiology, feed intake, and insulin related metabolism in growing pigs subjected to heat stress

2017 ◽  
Vol 1 (1) ◽  
pp. 116-125 ◽  
Author(s):  
F. Liu ◽  
J. J. Cottrell ◽  
U. Wijesiriwardana ◽  
F. W. Kelly ◽  
S. S. Chauhan ◽  
...  
Keyword(s):  
Heat Stress ◽  
Insulin Sensitivity ◽  
Respiration Rate ◽  
Rectal Temperature ◽  
Feed Intake ◽  
Insulin Response ◽  
Thermal Exposure ◽  
Growing Pigs ◽  
Model Assessment ◽  
Intravenous Glucose

Abstract Improving insulin sensitivity may reduce impacts of heat stress (HS) in pigs by facilitating heat dissipation. Chromium (Cr) has been reported to improve insulin sensitivity in pigs. Therefore, the aim of this experiment was to investigate whether Cr supplementation can mitigate HS in growing pigs. Thirty-six gilts were randomly assigned to 2 diets containing 0 (control) or 400 ppb Cr. After 14 d the supplemented pigs were allocated to either 8 d thermoneutral (20°C constant; TN) or cyclic HS (35°C, 0900 h to 1700 h) conditions and continued their respective diet (n = 9 per group). Growth performance was recorded during the 14-d supplementation period. The physiological responses to HS were monitored by measuring respiration rate, rectal temperature, blood gas chemistry, and feed intake during thermal exposure. Kinetics of plasma glucose, insulin and NEFA were studied by intravenous glucose tolerance test (IVGTT) on d 8 of thermal treatment. Results showed Cr alleviated the HS-increased rectal temperature (P &lt; 0.05) and respiration rate (P &lt; 0.01) at 1300 h and 1600 h during thermal exposure. However, Cr did not mitigate the reduction in average daily feed intake which was reduced by 35% during HS or the HS-induced respiratory alkalosis. Chromium tended to increase average daily gain (0.86 vs. 0.95 kg, P = 0.070) during the 14-d supplementation under TN conditions before thermal exposure, which might be associated with the potential of Cr in improving overall insulin sensitivity, as evidenced by a reduced insulin resistance index calculated by Homeostatic Model Assessment (HOMA-IR; 0.65 vs. 0.51, P = 0.013) and a tendency of reduced fasting plasma insulin concentration (1.97 vs. 1.67 μU/mL, P = 0.094). Heat stress decreased the acute insulin releasing rate (P = 0.012) and consequently slowed glucose clearance rate (P = 0.035) during IVGTT. Besides, HS enlarged the values of area under the curve of NEFA during IVGTT (P &lt; 0.01), indicating a reduced lipid mobilization. In conclusion, HS reduced insulin response to IVGTT. Chromium supplementation exhibited a potential in improving insulin sensitivity and mitigating HS symptoms in growing pigs.

scholarly journals Responses of Vaginal Microbiota to Dietary Supplementation with Lysozyme and its Relationship with Rectal Microbiota and Sow Performance from Late Gestation to Early Lactation

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 593
Author(s):  
Shengyu Xu ◽  
Yanpeng Dong ◽  
Jiankai Shi ◽  
Zimei Li ◽  
Lianqiang Che ◽  
...  
Keyword(s):  
Reproductive Performance ◽  
Control Diet ◽  
Dietary Supplementation ◽  
16S Rrna Gene Sequencing ◽  
Interleukin 10 ◽  
Fecal Microbiota ◽  
Vaginal Microbiota ◽  
Late Gestation ◽  
Rrna Gene ◽  
Early Lactation

This study was conducted to evaluate the effects of dietary lysozyme (LZM) supplementation on the vaginal microbiota, as well as the relationship between vaginal microbiota and the fecal microbiota of rectum and the reproductive performance of the sow. A total of 60 Yorkshire × Landrace sows (3–6 of parity) were arranged from day 85 of gestation to the end of lactation in a completely randomized design with three treatments (control diet, control diet + lysozyme 150 mg/kg, control diet + lysozyme 300 mg/kg). The results showed that sows fed with lysozyme increased serum interleukin-10 (IL-10, p < 0.05) on day 7 of lactation. The vaginal microbiota varied at different taxonomic levels with LZM supplementation by 16S rRNA gene sequencing. The most representative changes included a decrease in Tenericutes, Streptococcus, Bacillus and increase in Bacteroidetes, Actinobacteria, Enterococcus, and Lactobacillus (p < 0.05). There were 777 OTUs existing in both, vaginal and fecal microbiota. The addition of LZM also decreased the abundance of Tenericutes (p < 0.05) in the vagina and feces. The changes in the microbiota were correlated in some cases positively with the performance of the sow, for example, Bacillus in feces was positively correlated with the neonatal weight (p < 0.05). These results indicate that the addition of lysozyme to the diet of sow during perinatal period promote the change of vaginal bacterial community after farrowing. The variations in vaginal microbiota are also associated with the changes in the fecal microbiology of the rectum and the reproductive performance of the sow. Therefore, it is concluded that dietary supplementation with lysozyme in sows in late gestation stage until early lactation, is beneficial to establish vaginal microbiota that seems to promote maternal health and reproductive performance.

scholarly journals Dietary Supplementation of Some Antioxidants as Attenuators of Heat Stress on Chicken Meat Characteristics

Agriculture ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 638
Author(s):  
Mihaela Saracila ◽  
Tatiana Dumitra Panaite ◽  
Silvia Mironeasa ◽  
Arabela Elena Untea
Keyword(s):  
Heat Stress ◽  
Vitamin C ◽  
Crude Protein ◽  
Protein Concentration ◽  
Control Diet ◽  
Chicken Meat ◽  
Chromium Picolinate ◽  
Wood Powder ◽  
Breast Meat

The study evaluated the effect of dietary chromium and vitamin C, Zinc, and sorrel wood powder supplements on chicken health and the nutritional, textural, and sensorial quality of chicken meat. A total of 120 Cobb 500 chickens (heat stress, 32 °C) were assigned into four treatments: control diet (C) and three test diets including 200 µg/kg diet chromium picolinate and supplemented with: 0.25 g vitamin C(VC)/kg diet (E1), 0.025 g Zn/kg diet (E2), and 10 g creeping wood sorrel powder (CWS)/kg diet (E3). Crude protein concentration increased in the breast meat from the E3 group; crude fat decreased in E1 and E3 compared to those fed the C diet. Dietary combinations of CrPic with VC, Zn, and CWS increased redness and decreased the luminosity parameter of breast meat compared with the C group. Dietary combinations of CrPic with VC and CWS lowered the hardness of breast meat. Significant positive correlation was found between hardness–gumminess (r = 0.891), gumminess–cohesiveness (r = 0.771), cohesiveness–resilience-EE (r = 0.861; r = 0.585), ash-L* (r = 0.426), and a negative one between ash–a* (r = 0.446). In conclusion, a dietary combination of CrPic with VC, Zn, and CWS as antioxidant sources could have a beneficial effect on quality without affecting sensory attributes.

scholarly journals Effects of Dietary Supplementation of L-Carnitine and Excess Lysine-Methionine on Growth Performance, Carcass Characteristics, and Immunity Markers of Broiler Chicken

Animals ◽  
2019 ◽  
Vol 9 (6) ◽  
pp. 362 ◽  
Author(s):  
Seyed Ghoreyshi ◽  
Besma Omri ◽  
Raja Chalghoumi ◽  
Mehrdad Bouyeh ◽  
Alireza Seidavi ◽  
...  
Keyword(s):  
Amino Acids ◽  
Growth Performance ◽  
Feed Intake ◽  
Broiler Chicken ◽  
Control Diet ◽  
Carcass Characteristics ◽  
Dietary Supplementation ◽  
Dietary Treatment ◽  
Feed Consumption ◽  
Thoracic Vertebrae

L-carnitine as well as lysine and methionine are amino acids of important nutritional and nutraceutical interest and are used in nutritional strategies as dietary supplements to improve feed quality characteristics in animals and broiler chicken in particular. This study investigated the effect of different levels of L-carnitine and extra levels of lysine-methionine on growth performance, carcass characteristics, and some immune system markers. Two hundred seventy male Ross 308 broilers were a fed control diet (C) and eight different diets supplemented with an excess of amino acids. In the experimental diets, identified as D1, D2, D3, D4, D5, D6, D7, and D8, extra L-carnitine, lysine, and methionine were added in excess with respect to the American National Research Council (NRC) recommendations: L-carnitine equal to NRC (D1), control diet supplemented with lysine at 30% in excess of NRC, methionine at 30% in excess of NRC, and L-carnitine equal to NRC (D2), control diet supplemented with lysine equal to NRC, methionine equal to NRC, and L-carnitine at 15% in excess of NRC (D3), control diet supplemented control diet supplemented with lysine at 15% in excess of NRC, methionine at 15% in excess of NRC, and L-carnitine at 15% in excess of NRC (D4), control diet supplemented lysine at 30% in excess of NRC, methionine at 30% in excess of NRC, and L-carnitine at 15% in excess of NRC (D5), control diet supplemented with lysine equal to NRC recommendations, methionine equal to NRC recommendations, and L-carnitine at 75% in excess of NRC (D6), control diet supplemented with lysine at 15% in excess of NRC, methionine at 15% in excess of NRC, and L-carnitine at 75% in excess of NRC (D7), and control diet supplemented with lysine at 30% in excess of NRC, methionine at 30% in excess of NRC, and L-carnitine at 75% in excess of NRC (D8). During the starter and growth phases, feed intake was not affected by dietary treatment (p > 0.05). By contrast, body weight and FCR were both affected (p < 0.001) during the starter period. During the finisher phase, feed consumption was affected (p < 0.05) by dietary treatment. Feed intake of broilers fed on C, D3, D6, and D7 were statistically similar (p > 0.05) (1851.90, 1862.00, 1945.10, and 1872.80 g/pen/day, respectively) and were higher (p < 0.05) than 1564.40 g/pen/day (D5). With the exception of drumsticks, neck, back thoracic vertebrae, and proventriculus weights, the economical carcass segments were not affected (p > 0.05) by the dietary supplementation of amino acids. Duodenum and ileum weights and lengths decreased with amino acid supplementation (p < 0.05). IgT and IgG titers against Sheep Red Blood Cells (SRBC) for both primary and secondary responses were not affected by dietary treatments (p > 0.05). Dietary amino acids supplementation did not affect IgM titer after the secondary challenge (p > 0.05) and had a significant effect (p < 0.05) on serum antibody titers in broilers vaccinated against Newcastle disease (NCD) and Gumboro ‘s disease at the 27th and 30th days, respectively.

scholarly journals Effects of Herbal Adaptogen Feed-Additive on Growth Performance, Carcass Parameters, and Muscle Amino Acid Profile in Heat-Stressed Modern Broilers

2021 ◽  
Vol 12 ◽  
Author(s):  
Elizabeth S. Greene ◽  
Clay Maynard ◽  
Casey M. Owens ◽  
Jean-François Meullenet ◽  
Sami Dridi
Keyword(s):  
Body Weight ◽  
Heat Stress ◽  
Amino Acid ◽  
Growth Performance ◽  
Feed Intake ◽  
Control Diet ◽  
Amino Acid Profile ◽  
Poultry Production ◽  
Carcass Parameters ◽  
Cyclic Heat

Heat stress has strong adverse effects on poultry production and, thereby, threats its sustainability, which energized scientists to search for innovative and effective solutions. Here, we undertook this study to evaluate the effects of in-feed herbal adaptogen (stress response modifier) supplementation on growth performances, meat quality, and breast amino acid profile in chronic cyclic heat-stressed broilers. Day-old male Cobb 500 chicks (n = 720) were randomly assigned, in environmental chambers (n = 12, 24 pens), to three diet-treatments: a three-phase corn-soybean based diet fed as such (Control, C), or supplemented with the herbal adaptogen at 500 g/1000 kg control diet (NR-PHY-500) or at 1 kg/1000 kg control diet (NR-PHY-1000). From d29 to d42, birds from 9 chambers were exposed to cyclic heat stress (HS, 35°C from 9:30 am-5:30 pm), however, the rest of the chamber were maintained at thermoneutral conditions (24°C, TN), which creates 4 experimental groups: C-TN, C-HS, NR-PHY-500HS, and NR-PHY-1000HS (6 pens/group, 168 birds/group). HS altered growth performance via depression of feed intake and body weight. Adaptogen supplementation stimulated feed intake and averaged 65.95 and 83.25 g better body weight and 5 and 10 points better FCR at low and high dose, respectively, compared to heat-stressed birds. This increase in body weight was mirrored in enhanced weights of body parts (breast, tender, wings, and legs). Adaptogen supplementation modulated also breast amino acid profile, pH, color, and quality. Together, these data suggested that adaptogen supplementation could be a promising solution to alleviate heat stress, however further in-depth investigation for its mode of action and its underlying mechanisms are warranted.

scholarly journals Effects of Melatonin Supplementation during Pregnancy on Reproductive Performance, Maternal–Placental–Fetal Redox Status, and Placental Mitochondrial Function in a Sow Model

Antioxidants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1867
Author(s):  
Xie Peng ◽  
Xuelin Cai ◽  
Jian Li ◽  
Yingyan Huang ◽  
Hao Liu ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Reproductive Performance ◽  
Mitochondrial Function ◽  
Pregnancy Complications ◽  
Antioxidant Status ◽  
Control Diet ◽  
Dietary Supplementation ◽  
Underlying Mechanism ◽  
Redox Status ◽  
Mrna Levels

Melatonin (MT) is a bio-antioxidant that has been widely used to prevent pregnancy complications, such as pre-eclampsia and IUGR during gestation. This experiment evaluated the impacts of dietary MT supplementation during pregnancy on reproductive performance, maternal–placental–fetal redox status, placental inflammatory response, and mitochondrial function, and sought a possible underlying mechanism in the placenta. Sixteen fifth parity sows were divided into two groups and fed each day of the gestation period either a control diet or a diet that was the same but for 36 mg of MT. The results showed that dietary supplementation with MT increased placental weight, while the percentage of piglets born with weight < 900 g decreased. Meanwhile, serum and placental MT levels, maternal–placental–fetal redox status, and placental inflammatory response were increased by MT. In addition, dietary MT markedly increased the mRNA levels of nutrient transporters and antioxidant-related genes involved in the Nrf2/ARE pathway in the placenta. Furthermore, dietary MT significantly increased ATP and NAD+ levels, relative mtDNA content, and the protein expression of Sirt1 in the placenta. These results suggested that MT supplementation during gestation could improve maternal–placental–fetal redox status and reproductive performance by ameliorating placental antioxidant status, inflammatory response, and mitochondrial dysfunction.

Sign in / Sign up

Export Citation Format

Share Document