Comparative effects of ‘solid’-fat sources as a substitute for yellow grease on digestion of diets for feedlot cattle

Five cannulated Holstein steers fed a steam-flaked corn-based growing diet containing 40% of alfalfa hay were used in a 5 × 5 Latin square design to examine the effect of ‘solid’ supplemental fats as a substitute for yellow grease (YG) on the extent and site of digestion. Treatments were (% of diet DM) as follows: (1) no supplemental fat; (2) 5% YG; (3) 5.88% calcium soaps (ML); (4) 5% flaked palmitic acid (RP10); and (5) 5% hydrogenated palm fatty acid distillate (HPFAD). Supplemental fats replaced the corn in the control diet. Supplemental fat decreased (P < 0.01) ruminal and total-tract digestion of organic matter and tended to decrease (P = 0.06) ruminal digestion of neutral detergent fibre, with no effects on ruminal digestion of feed N, microbial N efficiency, or total-tract digestion of N and neutral detergent fibre. With the exception of RP10, fat supplementation decreased (P < 0.03) postruminal digestion of C18:0. Compared with the original C16:0:C18:0 ratio of solid fats, the C16:0:C18:0 ratio of fatty acids (FAs) entering the small intestine markedly decreased for all solid-fat treatments. Ruminal biohydrogenation of YG and ML were 73% and 49% respectively. On the basis of FA intake, postruminal FA digestion of YG, ML, RP10 and HPFAD was 0.97, 0.94, 0.92 and 0.80 of expected respectively. This experiment confirmed that postruminal digestion of total FAs of conventional supplemental yellow grease is a predictable function of total FA intake per unit of bodyweight. However, in the case sources of the solids fats, this relationship (FA intake and postruminal digestion of FAs) was less consistent. This may be due to their physical and chemical nature (saturatedFA:unsaturated FA ratio). On the basis of the nutrient digestion and postruminal FA digestibility observed in the present experiment, solid supplemental fats do not afford appreciable advantages over conventional YG when supplemented in growing diets (forage level ~400 g/kg diet DM) for feedlot steers.

Download Full-text

Digestion site and extent of carbohydrate fractions in steers offered by-product diets, as determined by detergent and enzymatic methods

Animal Science ◽

10.1017/s1357729800050190 ◽

1999 ◽

Vol 68 (1) ◽

pp. 173-182 ◽

Cited By ~ 5

Author(s):

K. Taniguchi ◽

Y. Zhao ◽

H. Uchikawa ◽

T. Obitsu

Keyword(s):

Crude Protein ◽

Dry Matter ◽

Control Diet ◽

Latin Square ◽

Soya Bean ◽

Italian Ryegrass ◽

Enzymatic Method ◽

Citrus Pulp ◽

Beet Pulp ◽

Ruminal Digestion

AbstractThe objective was to elucidate the influences of two analytical methods, which employed neutral detergent or enzymes, on the digestion of carbohydrate fractions in the rumen and post-rumen of steers. Structural carbohydrates in diets and digesta were directly measured using both procedures and the contents of non-fibre carbohydrates (NFC) and non-fibre non-starch polysaccharides (NFSP) were estimated by subtracting the structural carbohydrates and other components from the organic matter. Carbohydrate sources were Italian ryegrass and rolled barley in the control diet and, in each of other three diets, Italian ryegrass and rolled barley plus either beet pulp, citrus pulp or soya-bean hulls. All diets contained similar proportions of dry matter as crude protein, structural carbohydrates and NFC but the starch proportion of the NFC was different in each diet, as follows: control (0·62), beet pulp (0·48), citrus pulp (0·30) and soya-bean hulls (0·63). Four Holstein steers with ruminal, duodenal and Heal cannulas were offered the four diets in a 4 × 4 Latin-square design. Estimated digestibilities of the structural carbohydrates in the rumen and the whole tract were greater (P < 0·01) when the detergent method was used than when the enzymatic method was used. The maximal difference in the ruminal digestion among the four diets was only 0·05 by the detergent method but 0·17 by the enzymatic method. Conversely, the digestibility of NFC in the rumen, as estimated from the detergent structural carbohydrates was less (P < 0.01) than that estimated from enzymatic structural carbohydrates with no evident differences among the four diets. Starch digestibility in the rumen was extensive for all diets but the ruminal digestibility of NFSP was much lower in the control and soya-bean hull diets, especially when it was estimated using the detergent method. The duodenal flow of microbial protein was greater (P < 0·05) for the beet pulp and citrus pulp diets than for the control. The acetate concentration of the ruminal fluids ranged from 84 for the control diet to 128 mmol/l for the soya-bean hulls diet. The digestion of carbohydrate fractions and the fermentation characteristics in the rumen suggested that the enzymatic method is the more appropriate method for fractionating carbohydrates.

Download Full-text

Canola screenings as a fiber source in barley-based feedlot diets: effects on rumen fermentation and performance of steers

Canadian Journal of Animal Science ◽

10.4141/a99-065 ◽

2000 ◽

Vol 80 (1) ◽

pp. 161-168 ◽

Cited By ~ 5

Author(s):

S. J. Pylot ◽

J. J. McKinnon ◽

T. A. McAllister ◽

A. F. Mustafa ◽

J. Popp ◽

...

Keyword(s):

Control Diet ◽

Latin Square ◽

Dietary Treatment ◽

Barley Grain ◽

Feedlot Cattle ◽

Beef Steers ◽

Ruminal Fermentation ◽

Finishing Diets ◽

And Performance ◽

Barley Silage

Two experiments were conducted to determine the feeding value of canola screenings in combination with barley grain for beef steers. Four dietary treatments were used. These included canola screenings:barley grain ratios of 100:0; 75:25, 50:50, and 25:75 (as-fed basis). In a metabolic trial, the effects of dietary treatment on ruminal fermentation parameters were determined in a 4 × 4 Latin square design experiment using four ruminally fistulated steers. In a production trial, 66 individually fed steers were used in an 83-d finishing trial to determine the performance and carcass characteristics of feedlot cattle fed different levels of canola screenings. A barley grain/barley silage-based control diet was also fed for comparison purposes. Ruminal pH decreased (P < 0.05) while total volatile fatty acid concentrations increased (P < 0.05) as the level of barley grain in the diet increased. Ruminal NH3–N followed a pattern (P < 0.05) similar to that of pH. Inclusion level of canola screenings had no effect on DM intake. However, ADG and feed efficiency increased (P < 0.05) as the level of barley grain in the diet increased. Increasing the level of barley grain in the finishing diet decreased (P < 0.05) lean meat yield and increased (P < 0.05) carcass fat. It was concluded that canola screenings can be included as a source of fiber in barley-based diets. However, levels in excess of 500 g kg−1 reduced the performance of feedlot steers. Performance and cost of gain with 250 and 500 g kg−1 canola screenings in barley-based finishing diets were comparable with those fed an 800 g kg−1 barley grain and 200 g kg−1 barley silage diet. Key words: Canola screenings, ruminal fermentation, feedlot steer performance

Download Full-text

Feeding micronized and extruded flaxseed to dairy cows: Effects on digestion and ruminal biohydrogenation of long-chain fatty acids

Canadian Journal of Animal Science ◽

10.4141/a04-003 ◽

2004 ◽

Vol 84 (4) ◽

pp. 705-711 ◽

Cited By ~ 15

Author(s):

C. Gonthier ◽

A. F. Mustafa ◽

R. Berthiaume ◽

H. V. Petit ◽

D. R. Ouellet

Keyword(s):

Heat Treatment ◽

Fatty Acids ◽

Dairy Cow ◽

Dry Matter ◽

Control Diet ◽

Latin Square ◽

Long Chain Fatty Acids ◽

Ruminal Biohydrogenation ◽

Long Chain ◽

Matter Basis

Four lactating Holstein cows fitted with ruminal and duodenal cannulae were used in a 4 × 4 Latin square study to determine the effects of feeding micronized and extruded flaxseed on ruminal biohydrogenation (BH) and digestibility of fatty acids (FA) in the gastrointestinal tract. Four diets were formulated: a control diet with no flaxseed (C); a raw flaxseed diet (RF); a micronized flaxseed diet (MF); and an extruded flaxseed diet (EF). Flaxseed diets contained 126 g kg-1 flaxseed (dry matter basis). Experimental periods (n = 4) consisted of 21 d of diet adaptation and 7 d of data collection. Flaxseed supplementation increased (P < 0.05) intake of individual long-chain FA, reduced (P < 0.05) ruminal BH of total unsaturated C18, and increased (P < 0.05) duodenal flow of individual long-chain FA except for C18:2. Feeding flaxseed increased (P < 0.05) post-ruminal digestibility of C18:1, C18:2, and C18:3 and total tract digestibility of C16:0, C18:2, C18:3, and of total long-chain FA. Heat treatment had no effect on ruminal BH, duodenal flow and digestibility of unsaturated FA. Ruminal BH of C18:3 was higher (P < 0.05), while the duodenal flow of C18:3 was lower (P < 0.05) for cows fed EF than for those fed MF. Intake and duodenal flow of C16:0 were higher (P < 0.05) for cows fed MF relative to those fed EF. It was concluded that inclusion of flaxseed in dairy cow diets increased duodenal flow of polyunsaturated FA. However, heat treatments were not effective in protecting unsaturated FA from ruminal BH. Key words: Flaxseed, ruminal biohydrogenation, fatty acid, heat treatment

Download Full-text

Effects of dietary level of selenium and grain on digestive metabolism in lambs

Czech Journal of Animal Science ◽

10.17221/6823-cjas ◽

2013 ◽

Vol 58 (No. 6) ◽

pp. 253-261 ◽

Cited By ~ 11

Author(s):

O.E. Del Razo-Rodríguez ◽

Ramírez-BribiescaJE ◽

R. López-Arellano ◽

Revilla-VázquezAL ◽

S.S. González-Muñoz ◽

...

Keyword(s):

Organic Matter ◽

Latin Square ◽

Selenium Concentration ◽

Ruminal Fermentation ◽

Neutral Detergent Fibre ◽

Four Levels ◽

Ruminal Digestion ◽

Different Levels ◽

Matter Flow ◽

Corn Grain

The objective was to evaluate the effect of different levels of selenium with two levels of grain corn in the diets on ruminal, postruminal, and total tract digestion of nutrients, ruminal fermentation characteristics, and selenium balance in lambs. A split-plot design was used in four periods with repeated Latin square using eight Suffolk × Dorset male lambs with four levels of selenium (sodium selenite, Na2SeO3), without adding selenium, 0.3, 0.6, and 0.9 mg of selenium/kg dry matter (DM) with 70 and 50% of corn grain in the diet. The four selenium levels affected organic matter as follows: starch, neutral detergent fibre, nitrogen and selenium excretion; selenium ruminal digestion; organic matter, neutral detergent fibre, nitrogen and selenium postruminal digestion; and organic matter, neutral detergent fibre, nitrogen and selenium total tract digestion (P < 0.05). Starch and organic matter flow decreased with 0.6 and 0.9 mg of selenium with the 70% grain diet (P < 0.05). Selenium flow increased linearly with selenium levels (P < 0.01) with both levels of grain. Selenium supplementation increased linearly selenium concentration in ruminal fluid (P < 0.01), but did not affect other ruminal characteristics. Also, selenium absorption and retention was increased by selenium concentrations (P < 0.01). The apparent absorption of selenium increased (31%, P < 0.01) with the 70% grain diet. In conclusion, the high content of nonstructural carbohydrates improved the availability of selenium and 0.9 mg of selenium/kg DM improved the absorption and availability of selenium.

Download Full-text

Cassava root fermented with yeast improved feed digestibility in Brahman beef cattle

Animal Production Science ◽

10.1071/an15685 ◽

2017 ◽

Vol 57 (8) ◽

pp. 1613 ◽

Cited By ~ 2

Author(s):

C. Promkot ◽

P. Nitipot ◽

N. Piamphon ◽

N. Abdullah ◽

A. Promkot

Keyword(s):

Beef Cattle ◽

Dry Matter ◽

Control Diet ◽

Latin Square ◽

Nutrient Digestibility ◽

Dry Matter Intake ◽

Neutral Detergent Fibre ◽

Feed Digestibility ◽

Cassava Root ◽

Dietary Treatments

Saccharomyces cerevisiae has been used to enhance the protein level of low-protein feedstuffs such as cassava root. In the present study, fresh cassava root was grated and subjected to solid-state fermentation with S. cerevisiae to enhance the protein content. The fermentation process lasted for 21 days, followed by fermentation with yeast for 5 days. The fermented product was called yeast-fermented cassava root (YEFECAR). The YEFECAR was then evaluated as a feed ingredient at 10%, 20% and 30% dry matter in concentrate diet for dry matter intake and nutrient digestibility in non-pregnant female Brahman beef cattle (bodyweight 256 ± 11.5 kg). Four cattle were randomly given four treatments in 4 × 4 Latin square design for 21-day period, including a control diet (without fermented cassava root). Cattle were fed rice straw as roughage. The results showed that while dry matter intake was not significantly different among dietary treatments, average dairy gain tended to increase when fermented cassava root was fed at 20% in the concentrate diet. There were no significant differences in nutrient digestibility among the dietary treatments. However, neutral detergent fibre and acid detergent fibre digestibility showed an increasing trend when fermented cassava root was fed to the cattle. Likewise, fermented cassava root showed no effect on rumen microbial population, although rumen bacterial population and microbial protein tended to increase with YEFECAR addition. It was concluded that yeast-fermented cassava root at 20% in concentrate feed tended to enhance rumen bacteria population and neutral detergent fibre digestibility in cattle.

Download Full-text

Physical and Chemical Characteristics of Meats of the Indonesian Indigenous Crossbred Chickens Fed Fermented Mixture of Papaya (Carica papaya L.) Leaf and Seed Meal

International Journal of Veterinary Science ◽

10.37422/ijvs/029 ◽

2020 ◽

Keyword(s):

Crude Protein ◽

Control Diet ◽

Dietary Treatment ◽

Seed Meal ◽

Chemical Characteristics ◽

Breast Meat ◽

Ph Values ◽

Physical And Chemical Characteristics ◽

Dietary Treatments ◽

Physical And Chemical

The objective of this present study was to investigate the effect of feeding fermented mixture of papaya leaf and seed meal (FERM) on the physical and chemical characteristics of meats of the Indonesian indigenous crossbred chicken (IICC). The study was carried out with 300 day-old IICC. The chicks were randomly distributed to five treatment groups, i.e., CONT (control diet based on corn-soybean- diet), FERMA (diet containing 1% FERM), FERMB (2.5% FERM), FERMC (5% FERM) and FERMD (7.5% FERM). Each treatment group consisted of 6 replicates with 10 IICC in each. At week 8, one chick from each replicate was randomly taken and slaughtered. After being de-feathered and eviscerated, samples from breast and thigh meats were obtained. Results showed that the increased levels of FERM was followed by the increased (P<0.05) pH values, moisture and crude protein content of breast meats of the IICC. Dietary incorporation of FERM especially at the level of 2.5% increased (P<0.05) the content of fat in the breast meat of IICC, while further increased levels of FERM did not alter (P>0.05) the fat content of the IICC breast meat. Dietary treatment did not have any effect (P>0.05) on the lightness (L*) values of breast meat of the IICC. The redness (a*) values were higher (P<0.05) in FERMD breast meat than other. FERM diet resulted in lower (P<0.05) yellowness (b*) values in the IICC breast meat. The pH values and moisture content of thigh meat increased (P<0.05) with the increased level of FERM. The WHC decreased (P<0.05) with the elevated levels of FERM in the diets. There was an increase (P<0.05) in crude protein concentration in FERMA as compared to the other meats. Crude fat and ash concentrations in thigh meat were affected (P<0.05) by the treatments. Dietary treatments had no impact (P>0.05) on L* values of thigh meats. Feeding FERM at 7.5% from diets increased (P<0.05) and decreased (P<0.05) the redness and yellowness of meats. In conclusion, dietary inclusion of FERM especially at the level of 7.5% from diets improved the physical and chemical characteristics of the IICC meats.

Download Full-text

Menadione nicotinamide bisulphite as a source of vitamin K and niacin activities for the growing pig

Animal Science ◽

10.1017/s135772980005493x ◽

2000 ◽

Vol 71 (1) ◽

pp. 111-117 ◽

Cited By ~ 5

Author(s):

M. Marchetti ◽

M. Tassinari ◽

S. Marchetti

Keyword(s):

Vitamin K ◽

Control Diet ◽

Live Weight ◽

Organic Salt ◽

Free Form ◽

Food Supplementation ◽

Sodium Bisulphite ◽

Chemical Factors ◽

And Control ◽

Physical And Chemical

AbstractWhen compared with other menadione derivatives such as menadione sodium bisulphite (MSB), menadione nicotinamide bisulphite (MNB), an organic salt combining menadione and nicotinamide, shows better stability towards physical and chemical factors once it is added to pre-mixes or foods. The present work evaluates the bioavailability of the two vitamins present in this compound and toxicity in the pig. To assess vitamin bioavailability, pigs were given small amounts of food containing MNB or equivalent amounts of MSB and nicotinamide in the free form. Menadione and nicotinamide concentrations in blood samples drawn at set times after the diets were given did not reveal any significant differences between the two modes of administration. Haematic levels of both vitamins in animals receiving MNB, or MSB and nicotinamide, were after 2, 4, 8 and 12 h higher (P < 0·001) than those of untreated animals. The tolerance level to MNB was evaluated in pigs given diets containing graded amounts of MNB (100, 500, 2500 mg/kg) for 28 days. No significant (P > 0·05) differences were recorded in live weight, food intake and gain/food ratio in pigs given these diets when compared with those given an unsupplemented diet. Haemoglobin and bilirubin levels did not differ between animals given various amounts of MNB and control animals. Plasma aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities in pigs given 100 mg/kg of MNB did not show significant differences when compared with those observed in pigs given an unsupplemented control diet. In pigs on the diets supplemented with 500 and 2500 mg/kg of MNB there was a significant increase in the two enzymatic activities as compared with controls (P < 0·001 and P < 0·01). In the case of ALT this had disappeared by 28 weeks. MNB is a good source of vitamin K for the pig and does not appear to have any adverse effects, even when administered at levels higher than those normally used in pig food supplementation.

Download Full-text

Effects of feeding or abomasal infusion of canola oil in Holstein cows. 2. Gene expression and plasma concentrations of cholecystokinin and leptin

Journal of Dairy Research ◽

10.1017/s0022029904000299 ◽

2004 ◽

Vol 71 (3) ◽

pp. 288-296 ◽

Cited By ~ 13

Author(s):

Prasanth K Chelikani ◽

David R Glimm ◽

Duane H Keisler ◽

John J Kennelly

Keyword(s):

Gene Expression ◽

Feed Intake ◽

Canola Oil ◽

Unsaturated Fatty Acids ◽

Plasma Concentrations ◽

Latin Square ◽

Basal Diet ◽

Holstein Cows ◽

Abomasal Infusion ◽

Supplemental Fat

We determined the relative importance of cholecystokinin (CCK), leptin, and fatty acid concentrations in plasma in mediating the satiety effects of supplemental fat in lactating cows. Five ruminally and duodenally cannulated Holstein cows in late lactation were used in a 3×5 incomplete Latin square design with three treatments: 1) Control: basal diet (CON), 2) Control+supplementation of canola oil at 1 kg/d in the feed (FED) and 3) Control+abomasal infusion of canola oil at 1 kg/d (INF). Relative to CON, feed intake was reduced by INF but not by FED. We provide evidence that both FED and INF treatments stimulated CCK gene expression in the duodenum and elevated plasma CCK concentrations. However, our results did not support a role for CCK in mediating satiety through an endocrine mechanism of action. We speculate that CCK might be acting either through paracrine and/or neurocrine routes to influence feed intake in cattle. Both FED and INF had no effect on the mRNA abundance of leptin, lipoprotein lipase, or acetyl-CoA carboxylase in adipose tissue. Plasma concentrations of leptin, insulin and IGF-I were not altered by FED or INF, indicating that these signals may not be involved in mediating short-term hypophagic effects of dietary fat. Plasma concentrations of 18[ratio ]1n-9 and 18[ratio ]2n-6 were significantly greater for INF than for FED or CON. We conclude that the hypophagic effects of supplemental fat in cattle depend on the amount of unsaturated fatty acids reaching the intestine and that this satiety effect is mediated through CCK, oleic acid and (or) linoleic acid, but leptin is not involved.

Download Full-text

Effect of incremental amounts of fish oil in the diet on ruminal lipid metabolism in growing steers

British Journal Of Nutrition ◽

10.1017/s0007114510000292 ◽

2010 ◽

Vol 104 (1) ◽

pp. 56-66 ◽

Cited By ~ 26

Author(s):

K. J. Shingfield ◽

M. R. F. Lee ◽

D. J. Humphries ◽

N. D. Scollan ◽

V. Toivonen ◽

...

Keyword(s):

Fatty Acids ◽

Lipid Metabolism ◽

Fish Oil ◽

Latin Square ◽

Live Weight ◽

Dependent Manner ◽

Growing Cattle ◽

Potential Benefits ◽

Ruminal Biohydrogenation ◽

Long Chain

Based on the potential benefits to human health, there is interest in developing sustainable nutritional strategies to enhance the concentration of long-chainn-3 fatty acids in ruminant-derived foods. Four Aberdeen Angus steers fitted with rumen and duodenal cannulae were used in a 4 × 4 Latin square experiment with 21 d experimental periods to examine the potential of fish oil (FO) in the diet to enhance the supply of 20 : 5n-3 and 22 : 6n-3 available for absorption in growing cattle. Treatments consisted of total mixed rations based on maize silage fed at a rate of 85 g DM/kg live weight0·75/d containing 0, 8, 16 and 24 g FO/kg diet DM. Supplements of FO reduced linearly (P < 0·01) DM intake and shifted (P < 0·01) rumen fermentation towards propionate at the expense of acetate and butyrate. FO in the diet enhanced linearly (P < 0·05) the flow oftrans-16 : 1,trans-18 : 1,trans-18 : 2, 20 : 5n-3 and 22 : 6n-3, and decreased linearly (P < 0·05) 18 : 0 and 18 : 3n-3 at the duodenum. Increases in the flow oftrans-18 : 1 were isomer dependent and were determined primarily by higher amounts oftrans-11 reaching the duodenum. In conclusion, FO alters ruminal lipid metabolism of growing cattle in a dose-dependent manner consistent with an inhibition of ruminal biohydrogenation, and enhances the amount of long-chainn-3 fatty acids at the duodenum, but the increases are marginal due to extensive biohydrogenation in the rumen.

Download Full-text

Can diet change the diversity of ruminal bacterial species in beef cattle?

10.21203/rs.3.rs-20861/v1 ◽

2020 ◽

Author(s):

Hilda Silva Araujo de Melo ◽

Luis Carlos Vinhas Ítavo ◽

Alinne Pereira de Castro ◽

Camila Celeste Brandão Ferreira Ítavo ◽

Alexandre Menezes Dias ◽

...

Keyword(s):

Control Diet ◽

Bacterial Species ◽

Latin Square ◽

Diet Change ◽

Cellulolytic Bacteria ◽

Operational Taxonomic Units ◽

Proteolytic Bacteria ◽

Lactate Utilization ◽

And Control ◽

Lactic Bacteria

Abstract Background The objective of this study was to assess the effects of diet on bacterial species in the solid fraction of the ruminal content using the gene sequences of the conserved 16S rDNA region steers fed one of the following diets: canola (C), cottonseed (A), sunflower (G), soybean (SO), corn silage (S) and control diet (PD). Canola, cottonseed, sunflower and soybean were fed as whole seeds. Six crossbred steers (Body weight = 416.33 ± 93.30 kg; mean ± SD), castrated male, and fitted with ruminal cannula were used. The experimental design was a 6 × 6 Latin square design. Results Cellulolytic bacteria were predominant for all diets, with 47.75% of Operational Taxonomic Units (OTU) in animals fed the cottonseed diet. Amylolytic bacteria were identified for all diets, representing 62.51% OTU in animals consuming the sunflower diet. Proteolytic bacteria were identified for all diets, corresponding to 65.96% OUT in animals fed the sunflower diet. Lactic bacteria were identified for all diets. Megasphaera elsdenii bacterium was identified for all diets, with a greater diversity of this bacterium in steers fed the control diet. This bacterium may reduce the availability of hydrogen in the rumen due to propionate production and lactate utilization. Conclusion Oilseed in the diet showed a similarity of bacteria species with 47.5% of changing of the ruminal flora.

Download Full-text