scholarly journals Microbial degradation of polyethylene (PE)

2009 ◽  
Vol 27 (1) ◽  
pp. 66 ◽  
Author(s):  
Kiran Kumari ◽  
R. C. Aanad ◽  
Neeru Narula
Keyword(s):  
Degradation Rate ◽  
Enrichment Culture ◽  
Culture Technique ◽  
Bacterial Activity ◽  
Low Density Polyethylene ◽  
Co2 Evolution ◽  
Bacterial Isolates ◽  
Gram Negative ◽  
Soil Burial ◽  
Compost Treatment

Thirty two bacterial isolates were obtained from soil by soil burial method followed by enrichment culture technique in film culturing (FC) media. Bacterial isolates differing in morphology were selected, purified and maintained at 4°C. Thirty % of these isolates were found to be Gram negative and 50% showed positive starch hydrolysis test and were screened for their ability to degrade Low Density Polyethylene (untreated, UV and heat strips) in film culturing media and percent weight loss of polyethylene after 4th week was determined. Among various isolates, highest degradation was by Is 3, Is 22 and Is 31 to the range of 25–27%, of UV treated polyethylene strips. High temperature (40°C), was found to enhance degradation rate of polyethylene more effectively by 24–28% compared to low temperature at 30°C (18–21%). Degradation of treated polyethylene strips (UV, heat steam) was up to 4% by compost treatment as studied by using CO2 evolution, an estimation tool to analyze % degradation. Bacterial activity was also affected by various environmental factors like sunlight, temperature, oxygen etc.

scholarly journals Screening for Urease-Producing Bacteria from Limestone Caves of Sarawak

2016 ◽  
Vol 6 (1) ◽  
pp. 37-45 ◽  
Author(s):  
Armstrong Ighodalo Omoregie ◽  
Nurnajwani Senian ◽  
Phua Ye Li ◽  
Ngu Lock Hei ◽  
Dominic Ong Ek Leong ◽  
...  
Keyword(s):  
Chemical Reaction ◽  
Urease Activity ◽  
Enrichment Culture ◽  
Culture Technique ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Calcite Precipitation ◽  
Microbially Induced Calcite Precipitation ◽  
Key Enzyme ◽  
Urease Production

Urease is a key enzyme in the chemical reaction of microorganism and has been found to be associated withcalcification, which is essential in microbially induced calcite precipitation (MICP) process. Three bacterialisolates (designated as LPB19, TSB31 and TSB12) were among twenty-eight bacteria that were isolated fromsamples collected from Sarawak limestone caves using the enrichment culture technique. Isolates LPB19, TSB31and TSB12 were selected based on their quick urease production when compared to other isolates. Phenotypiccharacteristics indicate all three bacterial strains are gram-positive, rod-shaped, motile, catalase and oxidasepositive. Urease activity of the bacterial isolates were measured through changes in conductivity in the absence ofcalcium ions. The bacterial isolates (LPB19, TSB12 and TSB31) showed urease activity of 16.14, 12.45 and 11.41mM urea hydrolysed/min respectively. The current work suggested that these isolates serves as constitutiveproducers of urease, potentially useful in inducing calcite precipitates.

scholarly journals Molecular Diversity of Chlorpyrifos Degrading Bacteria Isolated from Apple Orchard Soils of Himachal Pradesh

2021 ◽  
Author(s):  
Anish Kumar Sharma ◽  
Jyotsana Pandit ◽  
Khyati Harkhani
Keyword(s):  
Molecular Diversity ◽  
Enrichment Culture ◽  
Biochemical Properties ◽  
Culture Technique ◽  
Apple Orchard ◽  
Bacterial Isolates ◽  
Degrading Bacteria ◽  
Pure Cultures ◽  
High Concentrations ◽  
Orchard Soils

A total of seventy-two bacterial isolates were obtained employing enrichment culture technique from apple orchard soils contaminated with chlorpyrifos. Pure cultures of bacterial isolates were obtained using streak plate method on mineral salt medium. Bacterial isolates were characterized on the basis of morphology, culture and biochemical properties. Six bacterial isolates exhibited high extracellular organophosphorus hydrolase activity along with high tolerance towards high concentrations of chlorpyrifos. Genomic DNA extraction from bacterial isolates was done with phenol/chloroform method. Molecular Diversity of six chlorpyrifos degrading bacterial isolates was done employing RAPD-PCR technique by using 25 decamer primers, where amplification was showed by only 20 primers. A total of 337 amplified bands and 64 unique bands ranging in size from 100 to 4900bps were produced after RAPD analysis. The similarity coefficient estimated by Jaccard’s coefficient for these bacterial isolates was found to range between 31 to 64 percent.

Biodegradation of low density polyethylene/starch films exposed to soil burial

2009 ◽  
Vol 34 (1) ◽  
pp. 41-48 ◽  
Author(s):  
Souad Djellalia ◽  
Nassima Benmahmoud ◽  
Tahar Sadoun
Keyword(s):  
Low Density Polyethylene ◽  
Low Density ◽  
Soil Burial ◽  
Starch Films

scholarly journals Bacterial Isolates and its Antibiotic Susceptibility Pattern in NICU

2014 ◽  
Vol 11 (1) ◽  
pp. 66-70 ◽  
Author(s):  
S Shrestha ◽  
NC Shrestha ◽  
S Dongol Singh ◽  
RPB Shrestha ◽  
S Kayestha ◽  
...  
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Neonatal Intensive Care Unit ◽  
Neonatal Intensive Care ◽  
Bacterial Isolates ◽  
Susceptibility Pattern ◽  
Gram Positive ◽  
Antibiotic Susceptibility Pattern ◽  
Gram Negative ◽  
Nosocomial Sepsis

Background Neonatal sepsis is one of the major causes of morbidity and mortality among the newborns in the developing world. Objectives To determine the common bacterial isolates causing sepsis in neonatal intensive care unit and its antibiotic susceptibility pattern. Methods A one year discriptive prospective study was conducted in neonatal intensive care unit to analyse the results of blood culture and to look into the sensitivity of the commonly used antibiotics. Results The blood culture yield by conventional method was 44.13% with nosocomial sepsis accounting for 10.79%. 84.08% were culture proven early onset sepsis and 15.95% were late onset sepsis. Klebsiella infection was the commonest organism isolated in early, late and nosocomial sepsis but statistically not significant. Gram positive organisms were 39.36% in which Staphylococcus aureus was the leading microorganism followed by coagulase negative staphylococcus areus. Gram negative organisms were 60.64% amongst them Klebsiella was the most often encountered followed by Pseudomonas. The most common organism Klebsiella was 87.5% and 78.3% resistance to ampicillin and gentamycin respectively. Among gram negative isolates 87.5% and 77.2% were resistance to ampicillin and gentamycin respectively. Among gram positive isolates 58.5% and 31.5% resistance were noted to ampicillin and gentamycin respectively. Resistance to cefotaxim to gram negative and gram positive isolates were 87.34% and 59.35% respectively. Conclusion Klebsiella is most common organism which is almost resistance to first line antibiotics. Resistance to both gram negative and gram positive isolates among firstline antibiotics and even with cefotaxim is emerging and is a major concern in neonatal intensive care unit. DOI: http://dx.doi.org/10.3126/kumj.v11i1.11030 Kathmandu University Medical Journal Vol.11(1) 2013: 66-70

scholarly journals Bacterial Isolates from Tracheal Aspirates and their Anti-microbial Susceptibility in Mechanically-Ventilated Children with Pneumonia Admitted to an Urban Critical Care Ward

2015 ◽  
Vol 2 (2) ◽  
pp. 60-64 ◽  
Author(s):  
KM Shahunja ◽  
Mohammed Abdus Salam ◽  
Tahmeed Ahmed ◽  
Pradip Kumar Bardhan ◽  
Shafiqul Alam Sarker ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Severe Pneumonia ◽  
Tracheal Aspirate ◽  
Diarrhoeal Disease ◽  
Multi Drug Resistance ◽  
Gram Negative Bacteria ◽  
Bacterial Isolates ◽  
Gram Negative ◽  
Mechanically Ventilated ◽  
Tracheal Aspirates

Background and Aims: Data on Bacterial isolates from tracheal aspirates in children with severe pneumonia requiring intubation and mechanical ventilation especially in developing countries are very limited. We examined the microbial spectrum of bacteria isolated from tracheal aspirate of those children. The antibiotic susceptibility profiles of those bacteria were also examined.Methods: We evaluated the data of all mechanically ventilated children aged 0-59 months admitted to Intensive Care Unit (ICU) of “Dhaka Hospital” of the International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b) between August 2009 and July 2013 having their tracheal aspirate culture done. Data were extracted from electronic medical records of the Dhaka Hospital.Results: Among 836 admitted pneumonia children in the ICU, we identified 35 children who fulfilled the inclusion criteria. Among them 34 (97%) had positive bacterial growths: Klebseilla species in 14 (40%), Escherichia coli in 11 (31%), Acinetobacter in 8 (23%) and Streptococcus species in 8 (23%). Additionally, Enterococcus, Staphylococcus aureus, Pseudominas and Proteus species were identified in 6 (17%), 4 (11%), and 2 (6%) of the children respectively. The susceptibility of the gram-negatives, except Klebseilla, to ampicillin, cotrimoxazole, gentamycin, ciprofloxacin, azithromycin, and ceftriaxone ranged from 0- 54%, while that for ceftazidime and amikacin ranged from 12-80%. The sensitivity of Klebseilla to these antibiotics ranged from 0-100%.Conclusions: Our data suggests that gram-negative bacteria, Klebseilla followed by Escherichia coli, and Acenetobacter are the predominant bacteria associated with severe pneumonia in ventilated children. The increased number of infections caused by Gram-negative bacteria is being accompanied by rising rates of multi-drug resistance which underscores the importance of aggressive antimicrobial therapy in the management of such children.Bangladesh Crit Care J September 2014; 2 (2): 60-64

scholarly journals Comparison of the Superpolymyxin and ChromID Colistin R Screening Media for the Detection of Colistin-Resistant Enterobacteriaceae from Spiked Rectal Swabs

2018 ◽  
Vol 63 (1) ◽  
Author(s):  
Delphine Girlich ◽  
Thierry Naas ◽  
Laurent Dortet
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Confidence Interval ◽  
Enterobacter Cloacae ◽  
Bacterial Isolates ◽  
Colistin Resistance ◽  
Gram Negative ◽  
Content Type ◽  
Rectal Swabs ◽  
Stool Samples

ABSTRACT The dissemination of carbapenemase-producing Enterobacteriaceae (CPE) has led to the increased use of colistin, which has resulted in the emergence of colistin-resistant Enterobacteriaceae worldwide. One of the most threatening scenarios is the dissemination of colistin resistance in CPE, particularly the plasmid-encoded resistance element MCR. Thus, it has now become mandatory to possess reliable media to screen for colistin-resistant Gram-negative bacterial isolates, especially Enterobacteriaceae. In this study, we evaluated the performances of the Superpolymyxin medium (ELITechGroup) and the ChromID Colistin R medium (bioMérieux) to screen for colistin-resistant Enterobacteriaceae from spiked rectal swabs. Stool samples were spiked with a total of 94 enterobacterial isolates (Escherichia coli, Klebsiella pneumoniae, Salmonella enterica, Enterobacter cloacae), including 53 colistin-resistant isolates. ESwabs (Copan Diagnostics) were then inoculated with those spiked fecal suspensions, and culture proceeded as recommended by both manufacturers. The sensitivity of detection of colistin-resistant Enterobacteriaceae was 86.8% (95% confidence interval [95% CI] = 74.0% to 94.0%) using both the Superpolymyxin medium and the ChromID Colistin R plates. Surprisingly, the isolates that were not detected were not the same for both media. The specificities were high for both media, at 97.9% (95% CI = 87.3% to 99.9%) for the Superpolymyxin medium and 100% (95% CI = 90.4% to 100%) for the ChromID Colistin R medium. Both commercially available media, ChromID Colistin R and Superpolymyxin, provide useful tools to screen for colistin-resistant Enterobacteriaceae from patient samples (rectal swabs) regardless of the level and mechanism of colistin resistance.

scholarly journals PREVALENCE OF CEPHALOSPORIN-RESISTANT GRAM-NEGATIVE BACILLI FROM CLINICAL SAMPLES

2016 ◽  
pp. 176
Author(s):  
Kavi Aniis ◽  
Rajamanikandan Kcp ◽  
Arvind Prasanth D
Keyword(s):  
Clinical Samples ◽  
Beta Lactamase ◽  
Bacterial Isolates ◽  
Beta Lactam ◽  
Gram Negative ◽  
Beta Lactamases ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Lactam Ring ◽  
Esbl Producers

<p>ABSTRACT<br />Objective: Beta-lactams are the group of antibiotics that contain a ring called as “beta-lactam ring,” which is responsible for the antibacterial activity.<br />The presence of resistance among Gram-negative organisms is due to the production of beta-lactamases enzymes that hydrolysis the beta-lactam ring<br />thereby conferring resistance to the organism. This study is undertaken to determine the prevalence of extended-spectrum beta-lactamase (ESBL)<br />producing Gram-negative organism from clinical samples.<br />Methods: A total of 112 clinical samples were taken for this study. The combined disc synergistic test (CDST) was used for the phenotypic detection<br />of ESBL producers from the clinical samples. The genotypic identification of ESBL producers was carried out by alkaline lysis method by isolation of<br />plasmid DNA.<br />Result: A total of 87 bacterial isolates were isolated and identified. Among them, Klebsiella (41%) was the predominant organism followed by<br />Escherichia coli (33%), Proteus (10%), Pseudomonas (10%), and Serratia (6%). Among the various bacterial isolates, Klebsiella showed a higher<br />percentage of resistance. The CDST showed that 8 isolates of Klebsiella, 3 isolates of E. coli, and 1 isolate of Pseudomonas were found to be ESBL<br />producers. The genotypic confirmation showed that the two bacterial isolates, namely, Klebsiella and E. coli were found to possess temoniera (TEM)<br />gene which was the 400-500 bp conferring resistance to the antibiotics.<br />Conclusion: The results of this study suggest that early detection of ESBL producing Gram-negative organism is a very important step in planning the<br />therapy of patient in Hospitals. CDST continues to be a good indicator in the detection of ESBL producers.<br />Keywords: Beta-lactamases, Gram-negative bacilli, Extended-spectrum beta-lactamase, Resistance, Combined disc synergistic test.</p><p> </p>

scholarly journals Characterisation of Plasmids in Multidrug Resistant Uropathogenic Gram-negative Bacterial Isolates

2021 ◽  
Author(s):  
Manish Ranjan ◽  
Mahadevan Kumar ◽  
Sourav Sen ◽  
Parijat Das ◽  
Kunal Kanti Lahiri
Keyword(s):  
Acquired Resistance ◽  
Multidrug Resistant ◽  
Cross Sectional Study ◽  
Bacterial Isolates ◽  
Cross Sectional ◽  
Gram Negative ◽  
Hospital Stays ◽  
Tract Infections ◽  
Almost All ◽  
Prolonged Hospital

Introduction: Urinary Tract Infections (UTI) are one of the commonest conditions for which people seek medical care with an estimated 150 million episodes per annum worldwide. An unprecedented upsurge in the rate of development of antimicrobial resistance has reduced the therapeutic options leading to increased morbidity, prolonged hospital stays, development of complications. Majority of these infections are attributable to Gram negative bacteria which have now acquired resistance to almost all classes of antibiotics. Aim: To analyse the plasmid-mediated drug resistance and characterise the major plasmid families that are in circulation. Materials and Methods: A cross-sectional study comprising of a total of 95 non consecutive multidrug-resistant gram-negative bacterial isolates were subjected to Plasmid based replicon typing from January 2017 to June 2018. The 18 major replicons were divided in five multiplex and three uniplex Polymerase Chain Reaction (PCR) formats and the samples were subjected for plasmid characterisation and further sequencing of the plasmid Deoxyribonucleic Acid (DNA). The data obtained was analysed by Microsoft Excel software. Results: Escherichia coli, accounted for maximum n=51 (53.7%), Klebsiella pneumoniae n=19 (20%), Citrobacter sp n=11 (11.6%), miscellaneous gram negative n=14 (14.7%) The isolates exhibited a high degree of resistance to almost all tested antibiotics, sparing a few like Fosfomycin, Chloramphenicol, Imipenem, Amikacin. A total of 154 different plasmid families were detected from the 95 isolates. FIB replicon (24%), FIA (21%), F, W (20%), FIC, B/O (14%), Y (12%), I1 replicon (10.5%) were the major plasmid families detected in the present study. Conclusion: Many isolates exhibited the presence of more than one Incompatibility (Inc.) group plasmids, conferring multidrug resistance to the isolates. The study highlights the need for further research to study the association between plasmid families and their respective antibiotic resistance profiles for a given geographical niche and the need to devise further methods to target these epidemic plasmids.

scholarly journals Isolation and Characterization of Pb Resistant Bacteria from Cilalay Lake, Indonesia

2015 ◽  
Vol 4 (3) ◽  
Author(s):  
Kesi Kurnia ◽  
Nina Hermayani Sadi ◽  
Syafitri Jumianto
Keyword(s):  
Heterotrophic Bacteria ◽  
Water Environment ◽  
Resistant Bacteria ◽  
Bacterial Isolates ◽  
Gram Negative ◽  
Isolation And Characterization ◽  
Agar Media ◽  
Physical And Chemical ◽  
Standard Disk

<span>Pollution of water environment with heavy metals is becoming one of the most severe environmental and human health hazards. Lead (Pb) is a major pollutant and highly toxic to human, animals, plants, and microbes. </span><span lang="IN">Toxic metals are difficult to remove from the environment, since they cannot be chemically or biologically degraded and are ultimately indestructible. Biological approaches based on metal-resistant microorganisms have received a great deal of attention as alternative remediation processes. </span><span>This study aim to isolat</span><span lang="IN">e</span><span> and characterize Pb resistant of heterotrophic bacteria in Cilalay Lake, </span><span lang="IN">West Java, </span><span>Indonesia. The water samples were collected </span><span lang="IN">along</span><span> three points around Cilalay Lake. </span><span lang="IN">Water physical and chemical </span><span>determination was performed using the Water Quality Checker</span><span lang="IN">. </span><span>The bacterial isolates were screened on T</span><span lang="IN">r</span><span>ipton</span><span lang="IN">e</span><span> Glucose Yeast (TGY) agar plates. </span><span lang="IN">Afterwards s</span><span>elected isolates were grown on Nutrient Agar media 50% </span><span lang="IN">with </span><span>supplemented Pb 100 ppm by the standard disk. Population of resistant bacteria was counted. The result from metal resistant bacteria indicated that all isolates w</span><span lang="IN">ere</span><span> resistant. The most abundant type of resistant </span><span lang="IN">bacteria </span><span>to lead was Gram negative more than Gram positive. Identified have metal resistant bacteria could be useful for the bioremediation of heavy metal contaminated sewage and waste water</span>

Sign in / Sign up

Export Citation Format

Share Document