Sustainability and income opportunities of farming milkfish (Chanos chanos) to local communities in Kenya: assessment of initial trials of earthen ponds.

Author(s):  
David Oersted Mirera ◽  
Charles C Ngugi

Abstract This case study documents the development of earthen pond mariculture from the late 1980s to 2009 in Kenya and prospects of community milkfish (Chanos chanos) culture using satellite farmers along the coast of Kenya, as well as challenges to this kind of farming and approaches towards sustainability. Pond mariculture was started in the 1980s by the Kenyan Fisheries Department through the onset of prawn farming (Penaeus indicus and P. monodon) in large scale demonstration ponds at Ngomeni under funding from FAO. The total culture area was estimated at 60 ha and milkfish occurred as a by-product of the prawn culture operations with indications of good growth. Similar observations were made for the milkfish in the artisanal Kwetu prawn culture pond (0.8 ha) that was started in the late 1990s, and one individual middle-scale farmer in Mtwapa creek (Wampare) who had interest in prawn culture at the time and constructed ponds covering an area of 3 ha. The initial recommendations for the potential to culture milkfish in Kenya were made through a research project (Mwaluma, 2002) whose objectives were to assess the potential of mud crab (Scylla serrata) pen culture in natural mangrove channels at Mtwapa creek. Intense participatory research into potential for milkfish culture coupled with extension was initiated at the Kwetu Training Centre along the Kenya coast with satellite groups in 2004. Consequently, improvements have since been achieved with wild seed collection in mangrove pools, use of chicken manure as organic pond fertilizer, and dependency of tidal water exchange, which has led to a maximum growth rate of 1.2 g/day. The demand for milkfish production has increased greatly among the coastal poor (Mirera, 2009b) and presently (Sept 2009) five communities have constructed more than 25 milkfish production ponds with a maximum production capacity of more than 70 kg per crop. These farmers have appreciated the contribution of milkfish culture to achieving food security and income generation for their families. The first harvest of milkfish in the community ponds were bought by the research/extension officer, and were fried and eaten together by all members of the community (including none group members) to demonstrate that milkfish can grow under local culture conditions. In subsequent harvests, the whole community is informed a day before the harvest, whereby they come to the farms during harvesting and buy the fish in quotas at two dollars per kg.

2017 ◽  
Vol 6 (8) ◽  
pp. 5459
Author(s):  
Chandra Teja K. ◽  
Rahman S. J.

Entomopathogenic fungi like Beauveria bassiana, Metarhizium anisopliae and Lecanicillium lecanii are used in biological control of agricultural insect pests. Their specific mode of action makes them an effective alternative to the chemical Insecticides. Virulent strains of Entomopathogenic fungi are effectively formulated and used as bio-insecticides world-wide. Amenable and economical multiplication of a virulent strain in a large scale is important for them to be useful in the field. Culture media plays a major role in the large-scale multiplication of virulent strains of Entomopathogens. Different substrates and media components are being used for this purpose. Yet, each strain differs in its nutritional requirements for the maximum growth and hence it is necessary to standardize the right components and their optimum concentrations in the culture media for a given strain of Entomopathogen. In the current study, three different nitrogen sources and two different carbon sources were tried to standardize the mass multiplication media for seven test isolates of Entomopathogenic fungi. A study was also conducted to determine the ideal grain media for the optimum conidial yields of the test isolates. Yeast extract was found to be the best Nitrogen source for the isolates. The isolates tested, differed in their nutritional requirements and showed variation in the best nitrogen and carbon sources necessary for their growth. Variation was also found in the optimum concentration of both the ingredients for the growth and sporulation of the isolates. In the solid-state fermentation study, rice was found to be the best grain for the growth of most of the fungi followed by barley. The significance of such a study in the development of an effective Myco-insecticide is vital and can be successfully employed in agriculture is discussed.


Plants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1610
Author(s):  
Branka Vinterhalter ◽  
Nevena Banjac ◽  
Dragan Vinterhalter ◽  
Dijana Krstić-Milošević

The hairy root clones of Gentiana dinarica cl-B, cl-D, cl-3, and cl-14 were cultivated in parallel in diverse simple bioreactors, including temporary immersion systems RITA® (TIS RITA®), bubble column bioreactors (BCB), and Erlenmeyer flasks (EF), and evaluated for biomass production and xanthone content. The obtained results showed that TIS RITA® and BCB containing ½ MS medium with 4% sucrose provided equally good growth conditions in which the majority of the clones displayed the higher percentage of dry matter (DM%), and xanthones norswertianin-1-O-primeveroside (nor-1-O-prim) and norswertianin production than those cultivated in EF. Thin and well branched hairy root clone cl-B grown in BCB for 7 weeks was superior regarding all growth parameters tested, including growth index (19.97), dry weight (2.88 g), and DM% (25.70%) compared to all other clones. Cl-B cultured in TIS RITA® contained the highest amount of nor-1-O-prim (56.82 mg per vessel). In BCB with constant aeration, cl-B accumulated the highest norswertianin content reaching 18.08 mg/vessel. The optimized conditions for cultivation of selected G. dinarica hairy root clones in highly aerated TIS RITA® and BCB systems contribute to the development of bioreactor technology designed for the large scale commercial production of xanthones nor-1-O-prim and norswertianin.


Author(s):  
G. I. Gorchakov ◽  
S. A. Sitnov ◽  
A. V. Karpov ◽  
I. A. Gorchakova ◽  
R. A. Gushchin ◽  
...  

Using maximum aerosol optical depth (MAOD) spatial distribution formation technique the optically dense haze expansion scales in period from 15 to 31 July 2016 over Eurasia are estimated in during great Siberian smoke haze (SSH) with the area 16 mln km2 about, smog over the Northern China Plain (2 mln km2), dust haze in Takla Makan desert (0.8 mln km2) and hazes in India and Pakistan (1 mln km2 approximately). Empirical distribution function (EDF) MAOD is received which is approximated by linear function of MAOD logarithm. Aerosol optical depth (AOD) spatial distribution at wavelength 550 nm in SSH is analyzed. Total smoke aerosol mass assessment in SSH (3.2 mln tons) is evaluated. Smoke aerosol (SA) mass during maximum growth period from 22 July to 26 July 2016 over Siberia (50°-70°, 60°-120 °E) was equal 2 mln tons approximately. Aerosol index (AI) temporal variability is illustrated visually SA composition qualitative change in SSH during long-range transport. It is shown that AI variations are correlated with AOD variations. Aerosol radiative forcing (ARF) at the top and the bottom of the atmosphere over Siberia from 22 July to 26 July 2016 is estimated (average ARF are equal –68 and –98 W/m2). EDF AOD and EDF ARF at the top of the atmosphere are approximated by exponential and power function of AOD correspondingly.


2021 ◽  
Vol 33 (5) ◽  
pp. 2863-2873
Author(s):  
Anna-Lena Höger ◽  
Carola Griehl ◽  
Matthias Noll

AbstractIn recent years microalgae products have developed increasing market demand, but sustainable industrial production is still challenged by biological stability of large-scale production plants. Yet the relationships between algal hosts, associated microbiomes, and contaminants in photobioreactors remains widely understudied. The aim of this study was to investigate the temporal development of microbiomes of four freshwater microalgae species Scenedesmus vacuolatus, Desmodesmus quadricauda, Chlorella sorokiniana, and Botryococcus braunii, in presence and absence of the zoosporic parasite Amoeboaphelidium protococcarum. To compare the effects of sterile and nonsterile culture conditions, infection experiments were performed in sterile laboratory (sterile) and simulated industrial conditions (open). Algal growth (dry weight, optical density, and nutrient consumption) was observed for 21 days, and samples of the associated microbiome were collected for bacterial 16S rRNA gene Illumina MiSeq sequencing. Infection patterns of A. protococcarum were algae species-specific, irrespectively of culture conditions. Bacterial community analysis demonstrated distinct and stable bacterial communities for each algae species, which were mostly dominated by α- and γ-Proteobacteria. Upon aphelid parasitosis, bacterial diversity increased, and community compositions diverged algae-specific over time. Moreover, bacterial functional traits shifted to detoxification, degradation, and cellulolysis once algae were infected. This study provides a first insight into the close connection between algae, associated bacterial microbiomes and appearing contaminants in photobioreactor systems.


2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Towfique Rahman ◽  
Md. Abdul Moktadir ◽  
Sanjoy Kumar Paul

Purpose Supply chains’ (SCs’) sustainability practices and recovery strategies are attaining popularity in academia and industries to improve the resilience of the SCs and to manage large-scale disruptions. The global pandemic caused by the COVID-19 has raised the question of the sustainability of essential health-care products’ SCs of Bangladesh. It is an essential avenue for making the life of people safe and secure. Despite its importance, most of the health-care SCs in Bangladesh are struggling to meet the demand of its nation due to capacity shortage, technological backwardness of the manufacturers, delivery capacity shortages and less advanced forecasting capabilities. Therefore, this study aims to investigate the key performance indicators (KPIs) of a sustainable recovery strategy in the context of health-care SCs considering the COVID-19 pandemic. Design/methodology/approach This study used a dynamic method named graph theory and matrix approach to evaluate the most critical KPIs of a sustainable recovery strategy in the context of Bangladeshi health-care SCs. Findings The result revealed that dynamic forecasting and planning with a smooth delivery and distribution support system, production capacity diversification and having alternative or multiple suppliers during extraordinary disruptions may aid in the sustainability of the health-care SCs in Bangladesh. Originality/value This study is unique as no previous study has identified and examined the sustainable recovery strategy perspective KPIs considering the COVID-19 pandemic in the context of Bangladeshi health-care SCs. This study will also add value by guiding decision-makers of the health-care SCs of Bangladesh to adopt strategies toward the sustainability of SCs.


Processes ◽  
2020 ◽  
Vol 8 (3) ◽  
pp. 324 ◽  
Author(s):  
Juan-Luis Fuentes ◽  
Zaida Montero ◽  
María Cuaresma ◽  
Mari-Carmen Ruiz-Domínguez ◽  
Benito Mogedas ◽  
...  

The large-scale biomass production is an essential step in the biotechnological applications of microalgae. Coccomyxa onubensis is an acidophilic microalga isolated from the highly acidic waters of Río Tinto (province of Huelva, Spain) and has been shown to accumulate a high concentration of lutein (9.7 mg g−1dw), a valuable antioxidant, when grown at laboratory-scale. A productivity of 0.14 g L−1 d−1 was obtained by growing the microalga under outdoor conditions in an 800 L tubular photobioreactor. The results show a stable biomass production for at least one month and with a lutein content of 10 mg g−1dw, at pH values in the range 2.5–3.0 and temperature in the range 10–25 °C. Culture density, temperature, and CO2 availability in highly acidic medium are rate-limiting conditions for the microalgal growth. These aspects are discussed in this paper in order to improve the outdoor culture conditions for competitive applications of C. onubensis.


2019 ◽  
Vol 31 (12) ◽  
pp. 1793 ◽  
Author(s):  
Valentina Lodde ◽  
Silvia Colleoni ◽  
Irene Tessaro ◽  
Davide Corbani ◽  
Giovanna Lazzari ◽  
...  

Several studies report that a two-step culture where mammalian oocytes are first kept under meiosis-arresting conditions (prematuration) followed by IVM is beneficial to embryo development. The most promising results were obtained by stratifying the oocyte population using morphological criteria and allocating them to different culture conditions to best meet their metabolic needs. In this study, horse oocytes were characterised to identify subpopulations that may benefit from prematuration. We investigated gap-junction (GJ) coupling, large-scale chromatin configuration and meiotic competence in compact and expanded cumulus–oocyte complexes (COCs) according to follicle size (<1, 1–2, >2cm) and season. Then we tested the effect of cilostamide-based prematuration in compact COCs collected from follicles <1 and 1–2cm in diameter on embryo development. Meiotic competence was not affected by prematuration, whereas COCs from follicles 1–2cm in diameter yielded embryos with a higher number of cells per blastocyst than oocytes that underwent direct IVM (P<0.01, unpaired Mann–Whitney test), suggesting improved developmental competence. Oocytes collected from follicles <1cm in diameter were not affected by prematuration. This study represents an extensive characterisation of the functional properties of immature horse oocytes and is the first report of the effects of cilostamide-based prematuration in horse oocyte IVM on embryo development.


Biomolecules ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1515
Author(s):  
Wiem Elloumi ◽  
Ahlem Jebali ◽  
Amina Maalej ◽  
Mohamed Chamkha ◽  
Sami Sayadi

Carotenoids have strong antioxidant activity as well as therapeutic value. Their production has been induced in algae under stressful culture conditions. However, the extreme culture conditions lead to the Programmed Cell Death (PCD) of algae, which affects their growth and productivity. This study was performed to evaluate the effect of salinity on the physiological and biochemical traits of Scenedesmus sp., thermal freshwater microalgae from Northern Tunisia. It was cultured under different NaCl concentrations ranging from 0 to 60 g/L. Results showed a good growth and high contents of total chlorophyll and carotenoids in Scenedesmus sp. cultured at 10 g/L of NaCl (salt-stressed 10 (Ss10)). The pigment composition of the Ss10 extract was acquired using HPLC–MS, and showed that the carotenoid fraction is particularly rich in xanthophylls. Moreover, the antioxidant (DPPH and FRAP) and enzymatic inhibition (tyrosinase and elastase) activities of the Ss10 extract were higher compared to those of the control culture. In addition, the cytotoxicity test on B16 cells showed that the Ss10 extract was non-toxic for all tested concentrations below 100 µg/mL. It also showed a rich unsaturated fatty acid (FA) composition. Therefore, these findings suggest that Scenedesmus sp. strain cultivated under mild stress salinity could be a source of biomolecules that have potential applications in the nutraceutical and cosmeceutical industries.


2019 ◽  
Vol 7 (10) ◽  
pp. 387 ◽  
Author(s):  
Monica Salamone ◽  
Aldo Nicosia ◽  
Giulio Ghersi ◽  
Marcello Tagliavia

Proteolytic enzymes are of great interest for biotechnological purposes, and their large-scale production, as well as the discovery of strains producing new molecules, is a relevant issue. Collagenases are employed for biomedical and pharmaceutical purposes. The high specificity of collagenase-based preparations toward the substrate strongly relies on the enzyme purity. However, the overall activity may depend on the cooperation with other proteases, the presence of which may be essential for the overall enzymatic activity, but potentially harmful for cells and tissues. Vibrios produce some of the most promising bacterial proteases (including collagenases), and their exo-proteome includes several enzymes with different substrate specificities, the production and relative abundances of which strongly depend on growth conditions. We evaluated the effects of different media compositions on the proteolytic exo-proteome of Vibrio alginolyticus and its closely relative Vibrio parahaemolyticus, in order to improve the overall proteases production, as well as the yield of the desired enzymes subset. Substantial biological responses were achieved with all media, which allowed defining culture conditions for targeted improvement of selected enzyme classes, besides giving insights in possible regulatory mechanisms. In particular, we focused our efforts on collagenases production, because of the growing biotechnological interest due to their pharmaceutical/biomedical applications.


1973 ◽  
Vol 51 (4) ◽  
pp. 693-699 ◽  
Author(s):  
Frederick Y. Kazama ◽  
Melvin S. Fuller

Pythium marinum Sparrow, isolated from lesions on Porphyra miniata, required Na+, Ca2+, and Mg2+ in quantities far exceeding those reported to be required by terrestrial species of Pythium. Unlike most of its terrestrial counterparts, P. marinum required alkaline pH values for good growth, with a pH of 7.8 providing maximum growth under the conditions examined. The requirement for large amounts of Na+ ions appeared to have a specific nutritional basis rather than a purely osmotic function. Growth at various temperatures indicated that the fungus is a facultative psychrophile with the optimum growth temperature between 15 and 20 °C. The nutritional data show that P. marinum is able to grow in a wide range of salt concentrations and is well adapted to the marine environment.


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