scholarly journals Modification of glucocorticoid-induced changes in myofibrillar protein turnover in rats by protein and energy deficiency as assessed by urinary excretion ofNT-methylhistidine

1984 ◽  
Vol 51 (3) ◽  
pp. 323-337 ◽  
Author(s):  
F. M. Tomas ◽  
A. J. Murray ◽  
L. M. Jones
Keyword(s):  
Body Weight ◽  
Food Intake ◽  
G Protein ◽  
Protein Turnover ◽  
Control Diet ◽  
Protein Energy Malnutrition ◽  
Myofibrillar Protein ◽  
Energy Restriction ◽  
Low Protein ◽  
Protein Energy

1. The effects of differing degrees of experimental protein-energy malnutrition on the response of myofibrillar protein turnover rates to administration of corticosteroid has been studied in two experiments on rats. The basal control diet, offeredad lib.in each case, contained 40 g protein/kg, and other groups received diets containing 62.5, 95 or 220 g protein/kg at 0.67, 1 or 1.5 times the level of the control energy intake.2. Daily administration of 25 or 30 mg corticosterone/kg body-weight after 18 d pre-feeding caused an increase in plasma protein, glucose and insulin concentrations, but a decrease in the corticosterone: insulin values. Liver size and protein content increased, as did the fractional excretion of dietary nitrogen as urea-N in all treated groups. However, whereas a fall in food intake and body-weight occurred in one experiment the reverse occurred in the other.3. NT-Methylhistidine excretion was 12% lower for rats receiving 40v.220 g protein/kg diet and excretion was increased by only 57v.90% respectively, when the two groups of rats were given 30 mg corticosterone/kg per d. Rats which received 25 mg corticosterone/kg per d and up to 95 g protein/kg diet increased excretion of NT-methylhistidine by an average 35%.4. The fractional degradation rate of myofibrillar protein (kd) was reduced by about 10% by the low-protein diet from 3.1 to 2.8%/d. During corticosterone treatment the increment inkdfor rats on this diet was only 60% of that for rats receiving the 220 g protein/kg diet, i.e. an increase of 1.8v.3.0%/d. Energy restriction further reducedkdduring low-protein intake but did not affect the response to the corticosterone. Variations in dietary protein from 40 to 95 g/kg had little effect on the increase inkdduring steroid treatment. The effect of corticosterone on calculated synthesis rates (kg) differed markedly between experiments. Whilekgfell by 50–65% in rats which lost weight on treatment, it rose by up to 60% in rats where carcass non-collagen-protein accretion remained unchanged or increased, despite an increase inkd5. Protein deficiency decreases the catabolic response to glucocorticoid, but the net metabolic response appears crucially dependent on changes in food intake or the stage of growth of the rat or both. A net anabolic response with increased fractional rates of myofibrillar protein breakdown, synthesis and accretion was observed in growing rats fed on relatively-low-protein diets and given 25 mg corticosterone/kg per d. This novel finding indicates that a particular role for cortisol in the adaptation to protein-energy malnutrition by humans should be ascribed only with caution.

Preventing protein-energy malnutrition after cortical stroke enhances recovery of symmetry in forelimb use during spontaneous exploration

2020 ◽  
Vol 45 (9) ◽  
pp. 1015-1021
Author(s):  
Larisa K. Matwee ◽  
Mariam Alaverdashvili ◽  
Gillian D. Muir ◽  
Jonathan P. Farthing ◽  
Sarah A. Bater ◽  
...  
Keyword(s):  
Body Weight ◽  
Food Intake ◽  
Infarct Size ◽  
Acute Phase ◽  
Acute Phase Response ◽  
Control Diet ◽  
Protein Energy Malnutrition ◽  
Post Stroke ◽  
Protein Energy ◽  
Cortical Stroke

Protein-energy malnutrition (PEM) commonly arises after stroke. We investigated the effects of preventing PEM on spontaneous recovery of forelimb use, infarct size, and the acute phase response in the chronic post-stroke period. Male, adult, Sprague–Dawley rats were acclimatized to control diet (12.5% protein), tested for pre-stroke forelimb use symmetry in the cylinder test, and exposed to photothrombotic cortical stroke or sham surgery. Food intake was monitored daily, and body weight weekly. Forelimb use was tested on day 4 after surgery, before assignment to control diet or PEM (0.5% protein), with subsequent testing on days 16 and 29. Blood, brain, and liver were collected on day 30. The low protein diet resulted in PEM, measured by decreased body weight (p < 0.001) and food intake (p = 0.016) and increased liver lipid (p < 0.001). Stroke (p = 0.016) and PEM (p = 0.001) independently elicited increases in serum α-2-macroglobulin concentration, whereas PEM alone decreased albumin (p < 0.001). PEM reduced recovery of forelimb use symmetry during exploration on days 16 (p = 0.024) and 29 (p = 0.013) but did not influence infarct size (p = 0.775). Stroke reduced reliance on the stroke-affected forelimb to initiate exploration up until day 29 (p < 0.001); PEM had no influence (p ≥ 0.463). Preventing post-stroke PEM appears to yield direct benefits for certain types of motor recovery. Novelty Preventing post-stroke malnutrition benefits certain types of motor recovery. An acute phase response may contribute to the poorer recovery with malnutrition.

Impact of protein energy malnutrition on Trichuris suis infection in pigs concomitantly infected with Ascaris suum

Parasitology ◽  
2002 ◽  
Vol 124 (5) ◽  
pp. 561-568 ◽  
Author(s):  
S. PEDERSEN ◽  
I. SAEED ◽  
K. F. MICHAELSEN ◽  
H. FRIIS ◽  
K. D. MURRELL
Keyword(s):  
Body Weight ◽  
Packed Cell Volume ◽  
Ascaris Suum ◽  
Protein Energy Malnutrition ◽  
Trichuris Suis ◽  
Intestinal Nematode ◽  
Low Protein ◽  
Protein Energy ◽  
Protein Diets ◽  
Eosinophil Counts

The objective of this experiment was to investigate a possible interaction between protein and energy malnutrition (PEM) and intestinal nematode infections. We report on a 3×2 factorial study in which pigs were fed either a low protein energy (LPE), low protein (LP) or a normal protein energy (NPE) diet, and 6 weeks later inoculated with Trichuris suis (4000 infective eggs). Secondarily, in order to obtain a polyparasitic status, pigs were concomitantly inoculated with Ascaris suum (600 infective eggs). The number of T. suis-infected pigs was higher in LP pigs compared with NPE pigs (100 versus 58%; P = 0·037), although the differences in median T. suis worm burdens between groups at necropsy 10 weeks post-infection (p.i.) (LPE: 795; LP: 835; NPE: 48 worms; P = 0·33) were not significant. Interestingly, only T. suis in NPE were highly aggregated (k = 0·44), in contrast to a more uniform distribution among pigs in LPE (k = 1·43) and LP (k = 1·55) i.e. the majority of pigs harboured moderate worm burdens in LPE and LP, while most pigs had few or no worms in NPE. Further, T. suis worms in the LPE and LP groups were decreased in length (mean: LPE: 23·5 mm; LP: 24·3 mm; NPE: 29·4 mm; P = 0·004). The pre-patency period of T. suis was also extended in the LPE and LP groups, as reflected by lower faecal egg output at week 6 (P = 0·048) and/or 7 p.i. (P = 0·007). More A. suum worms were recovered from LP compared with the NPE group (mean: 5·4 versus 0·6; P = 0·040); this was accompanied by a higher faecal egg output in the former (P = 0·004). The low protein diets resulted in lower pig body weight gains, serum albumin, haemoglobin and packed cell volume (PCV) levels as well as diminished peripheral eosinophil counts. Infection significantly altered these parameters in the low protein groups, i.e. the pathophysiological consequences of infection were more severe in the PEM pigs. These results demonstrate that reduced protein in the diet leads to malnourishment of both the host pigs and T. suis, and compromises the pig's ability to resist infection by T. suis and A. suum.

scholarly journals Pathogenesis of oedema in protein-energy malnutrition: the significance of plasma colloid osmotic pressure

1979 ◽  
Vol 42 (1) ◽  
pp. 21-31 ◽  
Author(s):  
Marta Fiorotto ◽  
W. A. Coward
Keyword(s):  
Interstitial Fluid ◽  
Control Diet ◽  
Protein Energy Malnutrition ◽  
Colloid Osmotic Pressure ◽  
Protein Diet ◽  
Water Filtration ◽  
Low Protein Diet ◽  
Oedema Formation ◽  
Low Protein ◽  
Protein Energy

1. Rats were made oedematous by feeding them low-protein diets (protein: energy (P:E) 0.005) ad lib., and measurements were made of plasma and interstitial fluid colloid osmotic pressures (πp and πi respectively) and interstitial fluid hydrostatic pressure (Pi) before, and at the onset of, oedema formation. Taken together as (πp − πi + Pi) these forces oppose capillary pressure (Pc) and thus determine rates of transcapillary water filtration. Interstitial fluid was sampled, in non-oedematous and oedematous animals, from perforated capsules implanted subcutaneously for the measurement of Pi. Blood, plasma and interstitial fluid volumes were also determined.2. In Expt I comparisons were made between animals fed on a control diet (P:E 0.210) and the lowprotein diet. In normal animals the ratio πp:πi was approximately 2, but in protein deficiency it was increased since reductions in the absolute value of πi matched those in πp. These changes were observed 2 weeks after the start of the experiment and became more exaggerated when oedema appeared (weeks 18–22).3. Pi was normally negative with respect to atmospheric pressure but increased to values close to zero when oedema formation occurred.4. Despite the reductions in πp that were seen in the protein-deficient animals the sum of the forces opposing filtration (πp − πi + Pi) did not change significantly during the experiment.5. Plasma and interstitial fluid volumes expressed per kg body-weight (measured using 125I-albumin and 35SO42−) were unchanged as πp initially decreased in the protein-deficient animals but increased markedly with the onset of oedema.6. In Expt 2 comparisons were made between animals fed the low-protein diet ad lib. and others fed on the control diet in restricted amounts so that weight loss was the same in the two groups of animals.7. The wasting induced by restriction of the control diet did not produce reductions in πp or πi and values for Pi were normal. Changes in the animals fed on the low-protein diet were similar to those observed in Expt I. By using 51Cr-labelled erythrocytes it was shown that the expansion in plasma volume that occurred when oedema appeared in the protein-deficient animals was mainly due to a reduction in total erythrocyte volume. Blood volume did not increase significantly.8. It was concluded that in the hypoproteinaemia induced in the experimental animals reductions in the value of πp, which might otherwise result in an imbalance of forces that would produce excessive rates of transcapillary water filtration, were compensated for by reductions in πi. Increases in Pi also compensated but were quantitatively less important.9. The significance of the results is discussed in terms of the pathogenesis of oedema in kwashiorkor and the concept of an oncotic threshold for oedema formation in hypoproteinaemia.

scholarly journals Myofibrillar protein turnover and urinary N-τ-methylhistidine output. Response to dietary supply of protein and energy

1975 ◽  
Vol 152 (3) ◽  
pp. 503-510 ◽  
Author(s):  
L N Haverberg ◽  
L Deckelbaum ◽  
C Bilmazes ◽  
H N Munro ◽  
V R Young
Keyword(s):  
Body Weight ◽  
Dietary Protein ◽  
Protein Turnover ◽  
Muscle Protein ◽  
Control Diet ◽  
Diet Group ◽  
Group Iii ◽  
Protein Energy ◽  
Body Weight Increase ◽  
Group Ii

The urinary excretion of total N-τ-methylhistidine by the growing rat was measured to evaluate the effects of dietary protein and energy restriction on muscle protein turnover in vivo. 2. Young male rats (about 100 g initial wt.) were fed on one of three diets. Group I (controls) received an adequate 18% lactalbumin diet for 28 days, on which they sustained maximum growth. Group II (protein-depleted) was fed for 14 days on 0.5 lactalbumin diet, which caused loss of weight; this was followed by repletion for 14 days with the control diet. Group III (protein-energy restricted) received a 1% lactalbumin diet at one-half the food intake of group II for 14 days, and this was also followed by 14 days of repletion with the control diet. 3. The controls showed a progressive rise in the daily urinary output of N-τ-methylhistidine, which was proportionally slightly less rapid than the body-weight increase. 4. The protein-depleted group II showed a marked and progressive decrease in N-τ-methylhistidine excretion, which was proportionally greater than the fall in body weight; during repletion, N-τ-methylhistidine output rose in parallel with body-weight increase, but it did not reach the value attained by the control group. 5. Group III, restricted in both dietary protein and energy, showed an initial small increase in daily N-τ-methylhistidine output, which contrasted with the sharp loss of body weight during this period. After 11 days on this restricted diet, group III then underwent a decrease in N-τ-methylhistidine output, which persisted into the first 4 days of the repletion period, after which output of the methylated amino acid became the same as for group II. 6. Creatinine output, used as an additional metabolic measure of muscle metabolism, showed a fairly constant relationship to body weight in groups I and II during depletion and repletion. However, rats with protein-energy deficiency (group III) underwent a marked increase in output of creatinine per unit of body weight, which also persisited into the repletion period before it fell to more normal values relative to body weight. 7. Analysis of the N-τ-methylhistidine content of actin isolated from a group of protein-depleted rats revealed a small (5%) but significance (P less than 0.02) decrease relative to well-nourished controls. 8. Hence, the rate of muscle protein degradation, as indicated by changes in urinary N-τ-methylhistidine output, appears to respond sensitively and in opposite directions to insufficiency of protein of energy in the diet.

scholarly journals Efek tepung ulat sagu (Rhynchophorus ferrugineus) terhadap penurunan kadar malondialdehyde (MDA) pada tikus Wistar dengan diet rendah protein

2021 ◽  
Vol 6 (2) ◽  
pp. 139
Author(s):  
Lara Ayu Lestari ◽  
M. Sulchan ◽  
Anang M Legowo ◽  
Kusmiyati Tjahjono ◽  
Achmad Zulfa Juniarto
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Protein Energy Malnutrition ◽  
Wilcoxon Test ◽  
Control Group ◽  
Rhynchophorus Ferrugineus ◽  
Group Of Seven ◽  
Low Protein ◽  
Protein Energy ◽  
One Way Anova

Kwashiorkor is protein-energy malnutrition (PEM) caused by protein deficiency. Sago worm flour (Rhynchophorus ferrugineus) can reduce malondialdehyde (MDA) caused by the high content of the amino acids glycine, lysine, and phenylalanine. The study aimed to prove the effect of sago worm flour on MDA levels in Wistar rats with a low protein diet. A true experimental-pre-post control group. The intervention was given for 28 days to 28 Wistar rats, divided into four groups with each group of seven rats. The dose of sago starch was 0,36 g/100 g rat body weight/day (P1), and a dose of 1,36 g/100 g rat body weight/day (P2) for rats fed a low diet protein. The treatment group was compared with a group of mice given a low diet protein (K +) and a group of healthy mice (K-). Serum MDA levels were measured by the TBARs method. Statistical analysis used paired t-test or Wilcoxon test and one-way ANOVA/Kruskal Wallis test. The results of decreasing MDA levels were in the P1 and P2 groups (p= 0,000). There was a difference in MDA levels in the P1 and P2 groups compared to the K + group (P= 0,000). There was a difference that decreased MDA levels between P1 and P2 (p= 0,000). In conclusion, sago worm flour at a dose of 0,36 g/100 g of rats/day and a dose of 1,36 g/100 g of body weight of rats/day can reduce MDA levels

scholarly journals Severe protein deficiency induces hepatic expression and systemic level of FGF21 but inhibits its hypothalamic expression in growing rats

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Joanna Moro ◽  
Catherine Chaumontet ◽  
Patrick C. Even ◽  
Anne Blais ◽  
Julien Piedcoq ◽  
...  
Keyword(s):  
Body Weight ◽  
Food Intake ◽  
Energy Expenditure ◽  
Energy Intake ◽  
Dietary Protein ◽  
Protein Deficiency ◽  
Protein Diet ◽  
Growing Rats ◽  
Low Protein ◽  
Protein Diets

AbstractTo study, in young growing rats, the consequences of different levels of dietary protein deficiency on food intake, body weight, body composition, and energy balance and to assess the role of FGF21 in the adaptation to a low protein diet. Thirty-six weanling rats were fed diets containing 3%, 5%, 8%, 12%, 15% and 20% protein for three weeks. Body weight, food intake, energy expenditure and metabolic parameters were followed throughout this period. The very low-protein diets (3% and 5%) induced a large decrease in body weight gain and an increase in energy intake relative to body mass. No gain in fat mass was observed because energy expenditure increased in proportion to energy intake. As expected, Fgf21 expression in the liver and plasma FGF21 increased with low-protein diets, but Fgf21 expression in the hypothalamus decreased. Under low protein diets (3% and 5%), the increase in liver Fgf21 and the decrease of Fgf21 in the hypothalamus induced an increase in energy expenditure and the decrease in the satiety signal responsible for hyperphagia. Our results highlight that when dietary protein decreases below 8%, the liver detects the low protein diet and responds by activating synthesis and secretion of FGF21 in order to activate an endocrine signal that induces metabolic adaptation. The hypothalamus, in comparison, responds to protein deficiency when dietary protein decreases below 5%.

Supplementation of a low protein diet in an attempt to optimize egg mass output

1991 ◽  
Vol 71 (1) ◽  
pp. 211-220 ◽  
Author(s):  
John D. Summers ◽  
J. L. Atkinson ◽  
D. Spratt
Keyword(s):  
Amino Acids ◽  
Body Weight ◽  
Soybean Meal ◽  
Egg Production ◽  
Corn Oil ◽  
Control Diet ◽  
Essential Amino Acids ◽  
Egg Mass ◽  
Minimum Requirement ◽  
Low Protein

Pullets were brought into production on a low-protein corn, soybean meal diet to which various nutrients including essential amino acids, choline, corn oil and feathermeal were supplemented. Production commenced between 18 and 20 wk of age and up to at least 60% production, hens fed the low protein diets (10% CP) produced at least as many eggs and as great an egg mass as these given a 17% protein corn, soybean meal control diet. Pullets fed the corn, soybean meal test diets, peaked at around 80% as compared to slightly over 90% for the control. Egg production immediately fell for birds fed the test diets to around 70% for most of the test treatments. Body weight remained constant or fell after peak production for birds fed the test diets as compared to a normal increase for birds on the control diet. Egg size increased for the test diets at a rate which was comparable to that of the control birds. In a second experiment, with older hens, supplementation of the 10% protein test diet with methionine, lysine, arginine and tryptophan, resulted in intakes of these amino acids which met NRC minimum requirement levels. However, egg mass output was reduced approximately 11% compared to the 17% protein control diet. While intakes of several essential amino acids fell below requirement levels, the degree that valine was calculated to be deficient in both experiments corresponded closely with the reduction in egg mass output of hens fed the test as compared to the control diet. Key words: Lysine, methionine, egg weight, body weight, hens.

scholarly journals Role of SOCS3 in POMC neurons in metabolic and cardiovascular regulation

2019 ◽  
Vol 316 (4) ◽  
pp. R338-R351 ◽  
Author(s):  
Zhen Wang ◽  
Jussara M. do Carmo ◽  
Alexandre A. da Silva ◽  
Kandice C. Bailey ◽  
Nicola Aberdein ◽  
...  
Keyword(s):  
Heart Rate ◽  
Body Weight ◽  
Food Intake ◽  
Stress Test ◽  
Control Diet ◽  
Negative Regulator ◽  
Cytokine Signaling ◽  
Male And Female ◽  
Air Jet ◽  
And Control

Suppressor of cytokine signaling 3 (SOCS3) is a negative regulator of leptin signaling. We previously showed that the chronic effects of leptin on blood pressure (BP) and glucose regulation are mediated by stimulation of proopiomelanocortin (POMC) neurons. In this study we examined the importance of endogenous SOCS3 in POMC neurons in control of metabolic and cardiovascular function and potential sex differences. Male and female SOCS3flox/flox/POMC-Cre mice in which SOCS3 was selectively deleted in POMC neurons and control SOCS3flox/flox mice were studied during a control diet (CD) or a high-fat diet (HFD) and during chronic leptin infusion. Body weight was lower in male and female SOCS3flox/flox/POMC-Cre than control mice fed the CD, despite similar food intake. Male SOCS3flox/flox/POMC-Cre mice exhibited increased energy expenditure. BP and heart rate were similar in male and female SOCS3flox/flox/POMC-Cre and control mice fed the CD. HFD-fed male and female SOCS3flox/flox/POMC-Cre mice showed attenuated weight gain. HFD-induced elevations in baseline BP and BP responses to an air-jet stress test were greater in female SOCS3flox/flox/POMC-Cre than control mice. Chronic leptin infusion produced similar responses for food intake, body weight, oxygen consumption, blood glucose, BP, and heart rate in all groups. Thus SOCS3 deficiency in POMC neurons influences body weight regulation in the setting of CD and HFD and differentially affects BP and energy balance in a sex-specific manner but does not amplify the dietary, glycemic, or cardiovascular effects of leptin.

scholarly journals Effect of short-term protein deprivation on hemopoietic functions of healthy volunteers

Blood ◽  
1980 ◽  
Vol 55 (4) ◽  
pp. 625-628 ◽  
Author(s):  
R Catchatourian ◽  
G Eckerling ◽  
W Fried
Keyword(s):  
Body Weight ◽  
G Protein ◽  
Protein Deprivation ◽  
Low Protein ◽  
Leukocyte Function ◽  
Leukocyte Chemotaxis ◽  
Complete Blood Counts ◽  
Excretion Rates

Abstract To ascertain the effects of protein deprivation on hemopoietic parameters in otherwise healthy subjects, three volunteers were placed on diets containing 0.15 g protein/kg body weight for 8 days followed in 2 mo by another 8-day study period during which they ingested their usual diets containing more than 0.9 g protein/kg body weight. Complete blood counts, serum protein determinations, and tests of in vitro and in vivo leukocyte chemotaxis were performed prior to and at the conclusion of each study period. Subjects were phlebotomized of 500 ml on day 7 of each study period. Twenty-four-hour urinary erythropoietin excretion rates were assayed just prior to and again postphlebotomy. Reticulocyte counts were performed at intervals up to 1 wk postphlebotomy. Some of these determinations were replicated during a subsequent study. The hemoglobin and hematocrits decrased slightly but significantly after 8 days on low protein diets. Erythropoietin excretion rates and reticulocyte responses to phlebotomy were also less marked while subjects were on protein depleted diets. Leukocyte chemotaxis, measured both in vitro and in vivo, was also markedly reduced while subjects were on protein-depleted diets. We conclude that 8 days of moderately severe protein deprivation significantly impairs erythropoiesis and leukocyte function in otherwise healthy individuals.

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