scholarly journals Efek tepung ulat sagu (Rhynchophorus ferrugineus) terhadap penurunan kadar malondialdehyde (MDA) pada tikus Wistar dengan diet rendah protein

2021 ◽  
Vol 6 (2) ◽  
pp. 139
Author(s):  
Lara Ayu Lestari ◽  
M. Sulchan ◽  
Anang M Legowo ◽  
Kusmiyati Tjahjono ◽  
Achmad Zulfa Juniarto
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Protein Energy Malnutrition ◽  
Wilcoxon Test ◽  
Control Group ◽  
Rhynchophorus Ferrugineus ◽  
Group Of Seven ◽  
Low Protein ◽  
Protein Energy ◽  
One Way Anova

Kwashiorkor is protein-energy malnutrition (PEM) caused by protein deficiency. Sago worm flour (Rhynchophorus ferrugineus) can reduce malondialdehyde (MDA) caused by the high content of the amino acids glycine, lysine, and phenylalanine. The study aimed to prove the effect of sago worm flour on MDA levels in Wistar rats with a low protein diet. A true experimental-pre-post control group. The intervention was given for 28 days to 28 Wistar rats, divided into four groups with each group of seven rats. The dose of sago starch was 0,36 g/100 g rat body weight/day (P1), and a dose of 1,36 g/100 g rat body weight/day (P2) for rats fed a low diet protein. The treatment group was compared with a group of mice given a low diet protein (K +) and a group of healthy mice (K-). Serum MDA levels were measured by the TBARs method. Statistical analysis used paired t-test or Wilcoxon test and one-way ANOVA/Kruskal Wallis test. The results of decreasing MDA levels were in the P1 and P2 groups (p= 0,000). There was a difference in MDA levels in the P1 and P2 groups compared to the K + group (P= 0,000). There was a difference that decreased MDA levels between P1 and P2 (p= 0,000). In conclusion, sago worm flour at a dose of 0,36 g/100 g of rats/day and a dose of 1,36 g/100 g of body weight of rats/day can reduce MDA levels

scholarly journals Expansion of the humoral effector cell compartment of both systemic and mucosal immune systemsin a weanling murine model which duplicates critical features of human protein-energy malnutrition

1996 ◽  
Vol 75 (3) ◽  
pp. 445-460 ◽  
Author(s):  
C.-L. Ha ◽  
L.E. Paulino ◽  
B.D. Woodward
Keyword(s):  
Epithelial Transport ◽  
Biological Fluids ◽  
Protein Energy Malnutrition ◽  
Human Protein ◽  
Control Group ◽  
High Concentration ◽  
Low Protein ◽  
Protein Energy ◽  
Mucosal Antibody ◽  
Zero Time

AbstractA direct comparison of systemic (spleen) and mucosal (intestine) antibody-producing systems was made in weanling male C57BL/6J mice subjected to wasting protein-energy malnutrition (PEM) by means of a low-protein protocol known to duplicate immunological and physiological features of human malnutrition. ELISA revealed low concentrations of biliary and gut lumen immunoglobulin (Ig) A in malnourished mice concomitantly with a high concentration of blood IgA. The low-protein model, therefore, exhibited fidelity to human protein-energy malnutrition in its influence on the concentrations of the mucosal Ig, IgA, in critical biological fluids. The number of IgA-, IgM- and IgG-containing cells was estimated morphometrically on a per organ basis. The low-protein protocol supported expansion in numbers of mucosal IgA-containing cells (18 x relative to a zero-time control group) and of splenic IgG- containing cells (135 x ), albeit an attenuated expansion in comparison with that of well-nourished control animals (132x and 571x respectively relative to zero-time controls). Up to terminal differentiation of Ig-containing cells, systemic and mucosal antibody-producing systems exhibited similarly remarkable resistance to wasting malnutrition. Epithelial transport of IgA may be an aspect of the mucosal antibody response which is particularly sensitive to PEM.

scholarly journals PENGARUH PEMBERIAN SAGU DIBANDING NASI TERHADAP BERAT BADAN TIKUS WISTAR

e-CliniC ◽  
2013 ◽  
Vol 3 (2) ◽  
Author(s):  
Eny A. Watumlawar ◽  
Sarah M. Warouw ◽  
Stefanus Gunawan
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Wilcoxon Test ◽  
Control Group ◽  
Increase Body Weight ◽  
Control Group Design ◽  
Tissue Mass ◽  
Group Design ◽  
Post Test ◽  
Male Wistar Rats

Abstract: Weight is one measure that gives description of the tissue mass including body fluids. Weight change is nfluenced by nutriment consumption. Sago contains high carbohydrates that can increase body weight. This study aimed to obtain the effect of sago compared to rice to body weight of wistar rats. This was a random laboratory experimental pre-post test with control group design. Subjects were male wistar rats, aged 5-6 months. The rats were divided in two groups: rice group as control and sago group. Sago was cooked in papeda form as much as 75 g of dried sago and 300 mL water. The rats were fed for 2 weeks. Data were analyzed by using the Wilcoxon test. The results showed that the sago group showed an increase of body weight significantly (p=0,001) meanwhile the rice group lose body weight significantly (p=0,001). Conclusion: Sago can increase body weight of wistar rats significantly.Keywords: body weight, sago, riceAbstrak: Berat badan merupakan salah satu ukuran yang memberikan gambaran massa jaringan termasuk cairan tubuh. Salah satu yang memengaruhi berat badan yaitu dengan mengonsumsi makanan bergizi. Sagu memiliki kandungan karbohidrat (pati) yang besar dan dapat meningkatkan berat badan. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian sagu terhadap berat badan dibanding nasi pada tikus wistar. Desain penelitian ini ialah eksperimental laboratorium acak pre-post test with control group design. Subjek penelitian ialah tikus wistar jantan berusia 5-6 bulan dibagi atas 2 kelompok: kelompok nasi (kontrol) dan kelompok sagu. Sagu dimasak dalam bentuk papeda sebanyak 75 g sagu kering dan 300 mL air. Tikus diperlihara selama 2 minggu. Berat badan diukur dengan menggunakan timbangan. Data dianalisis dengan uji Wilcoxon. Hasil penelitian menunjukkan bahwa pemberian sagu sebanyak 2 cc setiap hari selama seminggu mampu meningkatkan berat badan secara bermakna (p = 0,001) sedangkan pemberian nasi menurunkan berat badan (p=0,001). Simpulan: Pemberian sagu dapat meningkatkan berat badan tikus wistar secara bermakna.Kata kunci: berat badan, sagu, nasi

Impact of protein energy malnutrition on Trichuris suis infection in pigs concomitantly infected with Ascaris suum

Parasitology ◽  
2002 ◽  
Vol 124 (5) ◽  
pp. 561-568 ◽  
Author(s):  
S. PEDERSEN ◽  
I. SAEED ◽  
K. F. MICHAELSEN ◽  
H. FRIIS ◽  
K. D. MURRELL
Keyword(s):  
Body Weight ◽  
Packed Cell Volume ◽  
Ascaris Suum ◽  
Protein Energy Malnutrition ◽  
Trichuris Suis ◽  
Intestinal Nematode ◽  
Low Protein ◽  
Protein Energy ◽  
Protein Diets ◽  
Eosinophil Counts

The objective of this experiment was to investigate a possible interaction between protein and energy malnutrition (PEM) and intestinal nematode infections. We report on a 3×2 factorial study in which pigs were fed either a low protein energy (LPE), low protein (LP) or a normal protein energy (NPE) diet, and 6 weeks later inoculated with Trichuris suis (4000 infective eggs). Secondarily, in order to obtain a polyparasitic status, pigs were concomitantly inoculated with Ascaris suum (600 infective eggs). The number of T. suis-infected pigs was higher in LP pigs compared with NPE pigs (100 versus 58%; P = 0·037), although the differences in median T. suis worm burdens between groups at necropsy 10 weeks post-infection (p.i.) (LPE: 795; LP: 835; NPE: 48 worms; P = 0·33) were not significant. Interestingly, only T. suis in NPE were highly aggregated (k = 0·44), in contrast to a more uniform distribution among pigs in LPE (k = 1·43) and LP (k = 1·55) i.e. the majority of pigs harboured moderate worm burdens in LPE and LP, while most pigs had few or no worms in NPE. Further, T. suis worms in the LPE and LP groups were decreased in length (mean: LPE: 23·5 mm; LP: 24·3 mm; NPE: 29·4 mm; P = 0·004). The pre-patency period of T. suis was also extended in the LPE and LP groups, as reflected by lower faecal egg output at week 6 (P = 0·048) and/or 7 p.i. (P = 0·007). More A. suum worms were recovered from LP compared with the NPE group (mean: 5·4 versus 0·6; P = 0·040); this was accompanied by a higher faecal egg output in the former (P = 0·004). The low protein diets resulted in lower pig body weight gains, serum albumin, haemoglobin and packed cell volume (PCV) levels as well as diminished peripheral eosinophil counts. Infection significantly altered these parameters in the low protein groups, i.e. the pathophysiological consequences of infection were more severe in the PEM pigs. These results demonstrate that reduced protein in the diet leads to malnourishment of both the host pigs and T. suis, and compromises the pig's ability to resist infection by T. suis and A. suum.

Effect of Weight on Flour Okra Wistar Diabetes Rats

2021 ◽  
Vol 6 (3) ◽  
Author(s):  
Sukina B ◽  
Astati Astati ◽  
Ahmad Ahmad ◽  
Imanuddin Imanuddin
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Control Group ◽  
High Dose ◽  
Control Group Design ◽  
Test Analysis ◽  
Post Test ◽  
Study Population ◽  
Group 2 ◽  
One Way Anova

Diabetes mellitus is a disease that continues to increase in volume and is one of the main threats to the health of mankind. okra has phenols and flavonoids that have antioxidant effects and anti-diabetic effects are quercetin-3-O-ß-D-glucopyranosyl- (1 → 6) -SS-D-glucopyranoside and quercetin3-O-ß-D-4 '' - O-methyl-ß-D-glucopyranoside. This study aimed to determine the effect of flour weight okra against diabetic Wistar rats. This study was an experimental study using pre-post test design with control group design with simple random. The study population was a rat (Rattus norvegicus) Wistar male sex derived from animals that self-employed certified and provide health insurance in rats weighing 200 g-300 g (body weight adult). Random sampling of the population affordable. The results of this study indicate that the group average 1 BB increased by 1:56%, for the second group also increased by 1:40 BB% and in the control decreased by 1.49% BB. In group 1 and group 2 by treating starch suspension okra medium dose and high-dose, both an increase in weight by 3:00 grams. In the third group as the control group experienced a weight loss of 3 grams. The increase in mean body weight between the groups using the paired t test. Analysis to test different between groups by one-way ANOVA or one-way ANOVA. Results showed no effect of okra powder on body weight in diabetic Wistar rats between the control group and the treatment group. Keywords : Okra, weight, diabetes.

scholarly journals Pengaruh Kurang Tidur terhadap Berat Badan pada Tikus Wistar Jantan

2015 ◽  
Vol 4 (1) ◽  
Author(s):  
Deby Nelsya Eka Putri ◽  
Ellyza Nasrul ◽  
Machdawaty Masri
Keyword(s):  
Body Weight ◽  
Food Intake ◽  
Sleep Deprivation ◽  
Group Treatment ◽  
Wistar Rats ◽  
The Body ◽  
Control Group ◽  
Multiple Platform ◽  
Ad Libitum ◽  
One Way Anova

AbstrakPengurangan durasi tidur menurunkan kadar leptin dan meningkatkan kadar ghrelin sehingga merangsang nafsu makan dan meningkatkan kemungkinan terjadinya obesitas pada manusia. Pada tikus akan menyebabkan peningkatan asupan makanan tetapi terjadi penurunan berat badan yang disebabkan karena aktivitas yang tinggi pada tikus. Tujuan penelitian ini adalah untuk melihat pengaruh kurang tidur 24 jam, 48 jam dan 72 jam terhadap berat badan pada tikus Wistar jantan. Jenis penelitian adalah true experimental research dengan rancangan randomized post control group terhadap 14 ekor tikus Wistar yang dibagi atas kelompok kontrol, kelompok perlakuan 24 jam, 48 jam dan 72 jam. Tikus dikondisikan mengalami paradoxycal sleep deprivation dengan metode modified multiple platform. Asupan makanan diberikan ad libitum dan berat badan diukur setelah pengurangan durasi tidur selama 24 jam, 48 jam, dan 72 jam.Analisis data menggunakan uji Saphiro-Wilk Test dan One-Way ANOVA. Rerata berat badan setelah pengurangan durasi tidur 24 jam adalah 193,6±17,9 gram; setelah 48 jam 179,6±17,3 gram; dan setelah 72 jam 176,7±15,9 gram dibandingkan dengan kontrol 219.6±11,3 gram. Pengurangan durasi tidur 48 jam dan 72 jam dibandingkan dengan kontrol bermakna (p<0,05). Dapat disimpulkan bahwa terjadi penurunan berat badan pada pengurangan durasi tidur selama 48 jam dan 72 jam.Kata kunci: kurang tidur, berat badan, tikus wistarAbstractSleep deprivation lowers level of leptin and increases level of ghrelin which stimulates appetite and increases the likelihood of obesity in humans. In mice will increases food intake, but decreases the body weight due to high activity in mice. The objective of this study was to examine the effect of sleep deprivation 24 hours, 48 hours and 72 hours on body weight in male Wistar rats. This type of research was a true experimental design research with post randomized control group on 14 Wistar rats were divided into control group, treatment group 24 hours, 48 hours, and 72 hours. Rats conditioned paradoxycal sleep deprivation experienced by the modified multiple platform method. Given ad libitum food intake and body weight were measured after sleep deprivation for 24 hours, 48 hours, and 72 hours. Analysis of the data using the Shapiro-Wilk Test and One-Way ANOVA. The mean of body weight after 24 hour sleep deprivation was 193.6±17.9 g, after 48 hours was 179.6±17.3 g, and after 72 hours was 176.7±15.9 g compared with control was 219.6±11.3 g. Sleep deprivation 48 hours and 72 hours compared with controls was significant (p<0.05). It can be concluded there was reduction of body weight on sleep deprivation for 48 hours and 72 hours.Keywords: sleep deprivation, weight, rats

scholarly journals Modification of glucocorticoid-induced changes in myofibrillar protein turnover in rats by protein and energy deficiency as assessed by urinary excretion ofNT-methylhistidine

1984 ◽  
Vol 51 (3) ◽  
pp. 323-337 ◽  
Author(s):  
F. M. Tomas ◽  
A. J. Murray ◽  
L. M. Jones
Keyword(s):  
Body Weight ◽  
Food Intake ◽  
G Protein ◽  
Protein Turnover ◽  
Control Diet ◽  
Protein Energy Malnutrition ◽  
Myofibrillar Protein ◽  
Energy Restriction ◽  
Low Protein ◽  
Protein Energy

1. The effects of differing degrees of experimental protein-energy malnutrition on the response of myofibrillar protein turnover rates to administration of corticosteroid has been studied in two experiments on rats. The basal control diet, offeredad lib.in each case, contained 40 g protein/kg, and other groups received diets containing 62.5, 95 or 220 g protein/kg at 0.67, 1 or 1.5 times the level of the control energy intake.2. Daily administration of 25 or 30 mg corticosterone/kg body-weight after 18 d pre-feeding caused an increase in plasma protein, glucose and insulin concentrations, but a decrease in the corticosterone: insulin values. Liver size and protein content increased, as did the fractional excretion of dietary nitrogen as urea-N in all treated groups. However, whereas a fall in food intake and body-weight occurred in one experiment the reverse occurred in the other.3. NT-Methylhistidine excretion was 12% lower for rats receiving 40v.220 g protein/kg diet and excretion was increased by only 57v.90% respectively, when the two groups of rats were given 30 mg corticosterone/kg per d. Rats which received 25 mg corticosterone/kg per d and up to 95 g protein/kg diet increased excretion of NT-methylhistidine by an average 35%.4. The fractional degradation rate of myofibrillar protein (kd) was reduced by about 10% by the low-protein diet from 3.1 to 2.8%/d. During corticosterone treatment the increment inkdfor rats on this diet was only 60% of that for rats receiving the 220 g protein/kg diet, i.e. an increase of 1.8v.3.0%/d. Energy restriction further reducedkdduring low-protein intake but did not affect the response to the corticosterone. Variations in dietary protein from 40 to 95 g/kg had little effect on the increase inkdduring steroid treatment. The effect of corticosterone on calculated synthesis rates (kg) differed markedly between experiments. Whilekgfell by 50–65% in rats which lost weight on treatment, it rose by up to 60% in rats where carcass non-collagen-protein accretion remained unchanged or increased, despite an increase inkd5. Protein deficiency decreases the catabolic response to glucocorticoid, but the net metabolic response appears crucially dependent on changes in food intake or the stage of growth of the rat or both. A net anabolic response with increased fractional rates of myofibrillar protein breakdown, synthesis and accretion was observed in growing rats fed on relatively-low-protein diets and given 25 mg corticosterone/kg per d. This novel finding indicates that a particular role for cortisol in the adaptation to protein-energy malnutrition by humans should be ascribed only with caution.

scholarly journals Faulty Breast Feeding Practice : A Risk Factor in Malnourished Children

2015 ◽  
Vol 14 (2) ◽  
pp. 43-47
Author(s):  
Sukhendu Shekhar Sen ◽  
Jhulan Das Sharma ◽  
Dhananjoy Das ◽  
Shahed Iqbal ◽  
Md Badruddoza
Keyword(s):  
Breast Feeding ◽  
Protein Energy Malnutrition ◽  
Control Group ◽  
Case Group ◽  
Feeding Practice ◽  
Prelacteal Feeding ◽  
College Hospital ◽  
Major Health Problem ◽  
Protein Energy ◽  
Breast Fed

Background: The predominant form of malnutrition is commonly called proteincalorie malnutrition. Protein Energy Malnutrition (PEM) is still a major health problem in children of developing countries including Bangladesh. The causes of malnutrition are multifactorial including nutritional factors, socioeconomic factors, health status of the mothers and repeated infections in children.Objective: To explore the information regarding the breast feeding practices of children suffering from Protein-Energy Malnutrition.Methods: This case control study was conducted in Chittagong Medical College Hospital from November 2006 to April 2007. A total of 65 controls and 65 cases were selected consecutively for the purpose of the study. Their mothers were interviewed with help of structured questionnaire containing all the variables of interest to attain the study objectives. The test statistics used to analyze the data were descriptive statistics and Chi-square (c2) or Fisher’s Exact Probability Test.Results: A significantly higher frequency of cases (67.7%) were given pre-lacteal feed, predominantly honey and sugar-water compared to control group (41.5%) (p = 0.008). Nearly 100% of controls were given colostrums compared to 75% of the cases. About one-third (31.3%) of the cases was exclusively breast-fed in comparison to 58.5% of the control group (p = 0.003). Over onequarter (27.7%) of the control were breast-fed upto 6 months of age, as opposed to only 1.5% cases (p < 0.001). Duration of predominant breast feeding for more than 6 months of age was also significantly higher in control group than that in case group (p = 0.001). Breast milk substitutes demonstrate their significant presence in cases (38.5%) than that in controls (9%) (p = 0.003).Conclusion: The study showed that rejection of colostrums, practice of prelacteal feeding, delayed initiation of breast feeding, early cessation of exclusive breast feeding and use of formula milk all were significantly higher in the malnourished group of children than those in their normal counterpart.Chatt Maa Shi Hosp Med Coll J; Vol.14 (2); Jul 2015; Page 43-47

scholarly journals Regression of gestational diabetes induced cardiomegaly in offspring of diabetic rat

2009 ◽  
Vol 24 (4) ◽  
pp. 251-255 ◽  
Author(s):  
Honório Sampaio Menezes ◽  
Cláudio Galeano Zettler ◽  
Alice Calone ◽  
Jackson Borges Corrêa ◽  
Carla Bartuscheck ◽  
...  
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Weight Ratio ◽  
Diabetic Rats ◽  
Heart Weight ◽  
Diabetic Rat ◽  
Control Group ◽  
Computer Assisted ◽  
Significant Difference ◽  
Levene Test

PURPOSE: To compare body weight and length, heart weight and length, heart-to-body weight ratio, glycemia, and morphometric cellular data of offspring of diabetic rats (ODR) and of normal rats (control). METHODS: Diabetes was induced in 3 pregnant Wistar rats, bearing 30 rats, on the 11th day after conception by intraperitoneal injection of 50 mg/kg of streptozotocin. Six normal pregnant Wistar rats, bearing 50 rats, made up the control group. Morphometric data were obtained using a scale for the weight, length, heart and body measurements. Morphometric cellular data were obtained by a computer assisted method applied to the measurements of myocytes. Statistical analysis utilized Student's t-test, ANOVA and Levene test. RESULTS: Control offspring had greater mean body weight and length than offspring of diabetic rats (p < 0.001). Heart weight and length and heart-to-body ratios of newborn rats differed between groups at birth (p < 0.001), but showed no difference at 21 days. Mean nuclei area and perimetric value of the myocytes decrees throughout the first 21 days of life (p < 0.01) in the diabetic group. CONCLUSIONS: Heart hypertrophy on the offspring of diabetic rats at birth was demonstrated by the significant difference between the groups. After the eleventh day, no difference was found, which confirmed regression of cardiomegaly. The significant difference between the first and the 21th day of life, for nuclei area feature, demonstrate regression of cardiac hypertrophy in the offspring of diabetic rats.

scholarly journals Effects of proportional high-protein/low-carbohydrate formulated diet consumption in diabetic rats: Beneficial impact on glycemic and weight control

2020 ◽  
Vol 20 (07) ◽  
pp. 16984-16996
Author(s):  
MMC Anyakudo ◽  
◽  
DO Adeniji ◽  
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Treated Group ◽  
Morphological Features ◽  
High Protein ◽  
Control Group ◽  
Formulated Diet ◽  
Low Carbohydrate ◽  
Male Wistar Rats ◽  
And Control

The metabolic response to nutrient ingestion and the rate of digestion and absorption of nutrient molecules in bowel physiology plays an important role in the metabolic control of some human chronic non-infectious diseases. This experimentally-controlled designed nutritional study which lasted eight weeks aimed to determine the effects of proportional high-protein/low-carbohydrate (HP/LC) formulated diet on glycemic tolerance, glycemic control, body weight, organ weight and organ morphometry in healthy and diabetic adult male Wistar rats. Twenty-four male Wistar rats purchased from a disease-free stock were randomly categorized into four groups (n = 6, each) after two weeks acclimatization period in raised stainless steel cages with 6 mm2mesh floor and replaceable numbered blotters papers placed under each cage in a well-ventilated animal house. Animal groups include: Healthy control group (HC), Healthy treated group (HT), Diabetic control group (DC) and Diabetic treated group (DT. The animals were fed according to the experimental design with water ad libitumfor eight weeks. Diabetes was inducted with freshly prepared alloxan monohydrate solution (150 mg/kg bw, intraperitoneally). Body weights and fasting blood sugar concentrations were measured twice weekly, while oral glucose tolerance test was conducted on the last day of the eighth-week study and subsequently followed by organs extraction after anesthesia for weight and gross assessment. Proportional high-protein/low-carbohydrate formulated diet caused significant reduction in mean body weight of treated diabetic (DT: 22.6%; P= .001) and healthy (HT: 5.8%; P= .007) rats while the control animals on control diet recorded significant (P< .05) increase in body weight gain (DC: 12.4%; HC: 11.2%). Glycemic tolerance and control improved significantly in diabetic treated rats over that of the healthy treated rats. Gross morphometry of the extracted organs (kidneys, liver, heart, lungs, spleen and testes) revealed sustained normal morphological features without any visible lesion. In conclusion, consumption of proportional high-protein/low-carbohydrate formulated diet enhanced body weight reduction and sustained normal organ morphological features with good glycemic tolerance and control in experimental rats, suggesting its dietary potentiality, safety and suitability to ameliorate obesity-related diabetes.

scholarly journals Effects of aqueous leaf extract of Corchorus olitorius on the testis and blood testosterone level of adult Wistar rats

2021 ◽  
Vol 10 (2) ◽  
pp. 024-030
Author(s):  
Godwin Chinedu Uloneme ◽  
Demian Nnabuihe Ezejindu ◽  
Darlington Cyprian Akukwu ◽  
Amadi Chibundu Chiekezie
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Day Treatment ◽  
Control Group ◽  
Corchorus Olitorius ◽  
Group A ◽  
Normal Rat ◽  
Group B ◽  
Calcium Iron ◽  
Group D

Background: The extract of Corchorus olitorius has a reasonable content of vitamins A and C, calcium, iron and fibre, and therefore enjoys a universal application in the treatment of some disease conditions, even as the whole leaf is a very important component of food in so many cultures. Purpose: The study was designed to investigate the effect of Corchorus olitorius extracts on the testis of adult Wistar rats. Method: A total number of thirty two adult Wistar rats weighing between 180 and 200 grammes separated into four groups labeled A,B,C and D respectively were used for the study. Animals in group A which served as the control group were fed with the normal rat chow and water only. The group B rats were administered 100mg/kg body weight of aqueous extract of Corchorus olitorus; while those in group C were administered 500mg/kg body weight of the extract. The group D rats received 1000mg/kg body weight of the extract. For a period of four weeks, the different experimental animal groups received the respective aforementioned treatments once daily, around nine- o’clock in the morning through oral intubation. At the end of the 28 day treatment, the animals were sacrificed and the testes harvested for histological, investigation, and through cardiac puncture, blood samples for some hormonal studies was also collected and investigated using standard laboratory standards. Results: Observations made showed that the extract produced no histological distortions, degenerative or defective effects on the testicular tissues. The testosterone levels of group B, C, and D rats were observed to be significantly higher (P<0.005) than that of the group A (control group).ound: The extract of Corchorus olitorius has a reasonable content of vitamins A and C, calcium, iron and fibre, and therefore enjoys a universal application in the treatment of some disease conditions, even as the whole leaf is a very important component of food in so many cultures. Purpose: The study was designed to investigate the effect of Corchorus olitorius extracts on the testis of adult Wistar rats. Method: A total number of thirty two adult Wistar rats weighing between 180 and 200 grammes separated into four groups labeled A,B,C and D respectively were used for the study. Animals in group A which served as the control group were fed with the normal rat chow and water only. The group B rats were administered 100mg/kg body weight of aqueous extract of Corchorus olitorus; while those in group C were administered 500mg/kg body weight of the extract. The group D rats received 1000mg/kg body weight of the extract. For a period of four weeks, the different experimental animal groups received the respective aforementioned treatments once daily, around nine- o’clock in the morning through oral intubation. At the end of the 28 day treatment, the animals were sacrificed and the testes harvested for histological, investigation, and through cardiac puncture, blood samples for some hormonal studies was also collected and investigated using standard laboratory standards. Results: Observations made showed that the extract produced no histological distortions, degenerative or defective effects on the testicular tissues. The testosterone levels of group B, C, and D rats were observed to be significantly higher (P<0.005) than that of the group A (control group).

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