scholarly journals Isolation and identification of bioactive compounds from chloroform fraction of methanolic extract of Carissa opaca roots

2015 ◽  
Vol 30 (17) ◽  
pp. 2012-2016 ◽  
Author(s):  
Dildar Ahmed ◽  
Khaizran Fatima ◽  
Ramsha Saeed ◽  
Rashid Masih
Keyword(s):  
Bioactive Compounds ◽  
Methanolic Extract ◽  
Chloroform Fraction ◽  
Isolation And Identification ◽  
Carissa Opaca

scholarly journals Treating Hyperglycemia From Eryngium caeruleum M. Bieb: In-vitro α-Glucosidase, Antioxidant, in-vivo Antidiabetic and Molecular Docking-Based Approaches

2020 ◽  
Vol 8 ◽  
Author(s):  
Abdul Sadiq ◽  
Umer Rashid ◽  
Sadiq Ahmad ◽  
Mohammad Zahoor ◽  
Mohamed F. AlAjmi ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Free Radicals ◽  
Bioactive Compounds ◽  
Active Sites ◽  
Methanolic Extract ◽  
Array Detector ◽  
Chloroform Fraction ◽  
Ms Analysis

Natural-based drugs are believed to be safe, effective and economical. Based on the medicinal importance of the genus Eryngium and unexplored nature of Eryngium caeruleum, we have evaluated its antidiabetic and antioxidant potentials. Both in-vitro and in-vivo assays have been carried out for antidiabetic assays. The antioxidant activity was determined by using different free radicals [i.e., 1,1-diphenyl,2-picrylhydrazyl (DPPH), 2,2-azinobis[3-ethylbenzthiazoline]-6-sulfonic acid (ABTS), and hydrogen peroxide (H2O2)]. Moreover, different phytoconstituents were identified in the most active solvent fraction by GC-MS analysis. Furthermore, comparative fingerprints of methanolic extract and chloroform fraction were also analyzed via High Performance Liquid Chromatography coupled with Diode Array Detector (HPLC-DAD). The crude methanolic extract of E. caeruleum (Ec.Cr) and its sub-fractions [i.e., n-hexane (Ec.Hex), chloroform (Ec.Chf), ethyl acetate (Ec.EtAc), and aqueous (Ec.Aq) were employed in this study]. In the α-glucosidase inhibition assay, a concentration-dependent inhibitory response was observed against the enzyme. The most active sample was Ec.Chf which revealed an IC50 of 437 μg/ml in comparison to the standard acarbose (IC50 25 μg/ml). The rest of the samples showed moderate inhibition of α-glucosidase. In antioxidant assays, Ec.Chf and Ec.Cr exhibited a considerable scavenging effect against all the free radicals. The IC50 values recorded for Ec.Chf were 112, 109, and 150 μg/ml against DPPH, ABTS, and H2O2 respectively. Based on the in-vitro potential of Ec.Chf, this was subjected to the in-vivo model experiment. The Ec.Chf lowered the blood glucose level up to 10.3 mmol/L at 500 μg/Kg. The Ec.Chf was also subjected to GC-MS analysis. The GC-MS analysis confirmed the presence of 60 compounds. The identified phytoconstituents consist of some essential compounds previously reported with antidiabetic and antioxidant studies, which include thymol, tocopherol, phytol, nerolidol, (I)-neophytadiene, linolenic acid, and falcarinol. Similarly, the HPLC-DAD chromatograms of Ec.Cr and Ec.Chf exhibited a variety of peaks, which further demonstrates the possibility of important phytochemicals. In a nutshell, we can conclude that Eryngium caeruleum is a potential source of bioactive compounds which may be beneficial for the management of ailments like diabetes and free radicals mediated disorders. Molecular docking was performed to explore the possible role of all the identified bioactive compounds in the chloroform fraction of Eryngium caeruleum into active sites of the homology model of α-glucosidase.

Phytoceuticals in aniseeds: a practical approach for isolation and identification

2016 ◽  
Vol 5 (01) ◽  
pp. 4701
Author(s):  
Shobha R. I. ◽  
Andallu B.
Keyword(s):  
Chronic Diseases ◽  
Column Chromatography ◽  
High Performance ◽  
Methanolic Extract ◽  
Analytical Techniques ◽  
Therapeutic Agents ◽  
Systematic Research ◽  
Isolation And Identification ◽  
Traditional System ◽  
Pharmacologically Active

Nature has provided mankind with a broad and structurally diverse array of pharmacologically active chemical compounds, phytoceuticals, which have proved to be indispensable for the cure of chronic diseases or as lead structures for novel therapeutic agents. Almost, 70% of modern medicines in India have been developed from plants used in the traditional system of medicine. With the technological advances and the development of more sophisticated isolation and analytical techniques, there is great scope for further systematic research to screen and isolate many more phytoceuticals which might be more effective/as effective as synthetic drugs and thereby assess their potential in protecting against chronic diseases. The present investigation is aimed at isolation and identification of phytoceuticals in a spice, aniseed (Pimpinella anisum L), therapeutically less-exploited and widely used only for culinary purpose, using analytical techniques viz. column chromatography, high performance thin layer chromatography (HPTLC.) and nuclear magnetic resonance spectrometry. Among all the solvent fractions of methanolic extract tested, methanolic extract and ethyl acetate fraction possessed highest amounts of bioactive compounds viz. phenolics, flavonoids, flavonols which was confirmed by qualitative, quantitative and HPTLC. analyses. Spectral analysis using NMR. of one of the sub-fractions of aniseeds obtained by column chromatography, revealed the presence of a glycosylated flavone, luteolin-6C-glucoside in aniseeds.  The present study revealed aniseeds to be a great source of bioactive phytoceuticals which can be novel candidates for development of new therapeutic agents.

scholarly journals Lupeol acetate as a potent antifungal compound against opportunistic human and phytopathogenic mold Macrophomina phaseolina

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Shabnam Javed ◽  
Zaid Mahmood ◽  
Khalid Mohammed Khan ◽  
Satyajit D. Sarker ◽  
Arshad Javaid ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Ethyl Acetate ◽  
Oleanolic Acid ◽  
Vanillic Acid ◽  
Fungal Biomass ◽  
Protocatechuic Acid ◽  
Methanolic Extract ◽  
Antifungal Compound ◽  
Chloroform Fraction ◽  
Lupeol Acetate

AbstractAntifungal activity of Monothecabuxifolia methanolic extract and its various fractions were assessed against Macrophominaphaseolina, a soil-borne fungal pathogen of more than 500 vegetal species as well as rare and emerging opportunistic human pathogen. Different concentrations of methanolic extract (3.125 to 200 mg mL−1) inhibited fungal biomass by 39–45%. Isolated n-hexane, chloroform and ethyl acetate fractions suppressed fungal biomass by 32–52%, 29–50% and 29–35%, respectively. Triterpenes lupeol and lupeol acetate (1, 2) were isolated from n-hexane while betulin, β-sitosterol, β-amyrin, oleanolic acid (3–6) were isolated from chloroform fraction. Vanillic acid, protocatechuic acid, kaempferol and quercetin (7–10) were isolated from the ethyl acetate fraction and identified using various spectroscopic techniques namely mass spectroscopy and NMR. Antifungal activity of different concentrations (0.0312 to 2 mg mL−1) of the isolated compounds was evaluated and compared with the activity of a broad spectrum fungicide mancozeb. Different concentrations of mencozeb reduced fungal biomass by 83–85%. Among the isolated compounds lupeol acetate (2) was found the highest antifungal against M.phaseolina followed by betulin (3), vanillic acid (7), protocatechuic acid (8), β-amyrin (5) and oleanolic acid (6) resulting in 79–81%, 77–79%, 74–79%, 67–72%, 68–71% and 68–71%, respectively. Rest of the compounds also showed considerable antifungal activity and reduced M.phaseolina biomass by 41–64%.

scholarly journals Mammalian Arginase Inhibitory Activity of Methanolic Extracts and Isolated Compounds from Cyperus Species

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1694
Author(s):  
Kamel Arraki ◽  
Perle Totoson ◽  
Alain Decendit ◽  
Andy Zedet ◽  
Justine Maroilley ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Methanolic Extract ◽  
Significant Inhibition ◽  
Isolation And Identification ◽  
Vasorelaxant Effect ◽  
Methanolic Extracts ◽  
Phytochemical Investigation ◽  
Ic50 Values ◽  
First Time

Polyphenolic enriched extracts from two species of Cyperus, Cyperus glomeratus and Cyperus thunbergii, possess mammalian arginase inhibitory capacities, with the percentage inhibition ranging from 80% to 95% at 100 µg/mL and 40% to 64% at 10 µg/mL. Phytochemical investigation of these species led to the isolation and identification of two new natural stilbene oligomers named thunbergin A-B (1–2), together with three other stilbenes, trans-resveratrol (3), trans-scirpusin A (4), trans-cyperusphenol A (6), and two flavonoids, aureusidin (5) and luteolin (7), which were isolated for the first time from C.thunbergii and C. glomeratus. Structures were established on the basis of the spectroscopic data from MS and NMR experiments. The arginase inhibitory activity of compounds 1–7 was evaluated through an in vitro arginase inhibitory assay using purified liver bovine arginase. As a result, five compounds (1, 4–7) showed significant inhibition of arginase, with IC50 values between 17.6 and 60.6 µM, in the range of those of the natural arginase inhibitor piceatannol (12.6 µM). In addition, methanolic extract from Cyperus thunbergii exhibited an endothelium and NO-dependent vasorelaxant effect on thoracic aortic rings from rats and improved endothelial dysfunction in an adjuvant-induced arthritis rat model.

scholarly journals Exploration of Hepatoprotective Potential and Phytochemicals of Ziziphus oxyphylla Edgew

2020 ◽  
Vol 40 (04) ◽  
pp. 431-436
Author(s):  
Abdullah .
Keyword(s):  
Superoxide Dismutase ◽  
Methanolic Extract ◽  
Histopathological Examination ◽  
Liver Weight ◽  
2D Nmr ◽  
Chloroform Fraction ◽  
Hypotonic Solution ◽  
2D Nmr Spectroscopy ◽  
Hepatic Diseases ◽  
Antihepatotoxic Activity

Ziziphus oxyphylla Edgew (ZO) has been traditionally used for the treatment of hepatic diseases in Pakistan. The current study was carried out to investigate the effectiveness and mechanism underlying hepatoprotective effect of ZO. Hepatoprotective potential of root extracts (crude methanolic extract and subsequent fractions) was investigated against carbon tetrachloride (CCl4) induced hepatotoxicity in BALB/c mice via determination of serum biomarkers, antioxidant enzymes (catalase, superoxide dismutase), lipid peroxidation and histopathology. Membrane stabilizing activity was determined via inhibition of hypotonic solution and heat-induced hemolysis. Crude methanolic extract and chloroform fraction ameliorated CCl4 induced elevated levels of alanine transaminase (ALT), alkaline phosphatase (ALP), total proteins (TP), total bilirubin (TB) and reversed the antioxidants imbalance in terms of catalase (CAT), superoxide dismutase (SOD) and reduced malondialdehyde (MDA) level. Crude methanol extract inhibited hemolysis induced by hypotonic solution and heat. Histopathological examination of liver specimens of extracts administered mice exhibited recovery from CCl4 induced pathological changes. Moreover, CCl4 induced alteration in body and liver weight was countered by the administration of ZO. Compounds betulinic acid (AZO1), ceanothic acid (AZO2) and zizybrenalic acid (AZO3) were isolated from chloroform fraction of the plant. The structures of compounds were deduced using 1D-and 2D-NMR spectroscopy techniques. The results show that Ziziphus oxyphylla roots extract possess antihepatotoxic activity mediated by the protection of anti-oxidant defense system and membrane-stabilizing activity, probably due to the presence of pentacyclic triterpenes

scholarly journals ISOLATION OF CYTOTOXIC CONSTITUENT FROM BIOACTIVITY GUIDED FRACTION OF ALYSICARPUS MONILIFER L. (DC.)

2019 ◽  
pp. 69-77
Author(s):  
PURVI HARISHKUMAR KAKRANI ◽  
HARISH KAKRANI ◽  
MANAN RAVAL
Keyword(s):  
Growth Inhibition ◽  
Cytotoxic Activity ◽  
Cancer Cells ◽  
Betulinic Acid ◽  
Breast Cancer Cells ◽  
Methanolic Extract ◽  
Chloroform Fraction ◽  
Tumor Removal ◽  
Aerial Parts ◽  
Mcf 7

Objective: Alysicarpus monilifer (Family Papilionaceae) has been used in the Indigenous system of medicine in tumor removal. The present study was designed to isolate and identify the constituent responsible for cytotoxic (anti-tumor) effects of the plant Alysicarpus monilifer. Methods: The plant was powdered and extracted to give a methanolic extract. Initially, Hexane, chloroform, ethyl acetate and methanolic fractions of the methanolic extract of the plant were subjected to cytotoxic screening using cell line based assay (MTT assay and NRU assay). The chloroform fraction showed significant cytotoxicity, so it was further subjected to column chromatography, to separate the cytotoxic phytoconstituent. The cell lines selected were breast cancer cells (MCF-7 and MDA-MB-468) and Liver cancer cells (HepG2 and HLE cell). Results were calculated as percentage growth inhibition with respect to untreated (control) cells versus treated cells. Result: A triterpene, Betulinic acid, was isolated from the aerial parts of Alysicarpus monilifer. The cytotoxic activity of the identified compound against MCF-7, MDA-MB-231, HLE and HepG2 cells was also found to be highly significant with 90% growth inhibition. Conclusion: The triterpene was identified to be betulinic acid, to which the cytotoxic activity can be attributed. It is a first report of isolation of betulinic acid from the Alysicarpus species.

Effect-Directed Discovery of Bioactive Compounds Followed by Highly Targeted Characterization, Isolation and Identification, Exemplarily Shown forSolidago virgaurea

2016 ◽  
Vol 88 (16) ◽  
pp. 8202-8209 ◽  
Author(s):  
Ágnes M. Móricz ◽  
Péter G. Ott ◽  
Tim T. Häbe ◽  
András Darcsi ◽  
Andrea Böszörményi ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Isolation And Identification
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