Measurement of Scale Protein Synthesis as an Index of Specific Growth                Rate in Oreochromis niloticus (L) and Labeo dussemerii (Valenciennes)

ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh dari hibridisasi ikan nila Pandu F6 dengan nila Nilasa terhadap karakter reproduksinya dan performa benih yang dihasilkan. Penelitian ini dilakukan dengan metode eksperimental menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 3 kali ulangan. Perlakuan dalam penelitian ini: perlakuan A (Pandu F6 ♀><Pandu F6 ♂), B (Nilasa ♀><Nilasa ♂), C (Pandu F6 ♀>< Nilasa ♂), dan D (Nilasa ♀><Pandu F6 ♂). Data yang diamati meliputi fekunditas, daya tetas telur, diameter dan bobot telur, panjang dan bobot larva TL, panjang dan bobot larva lepas kuning telur, kelulushidupan, laju pertumbuhan spesifik, konversi pakan dan kualitas air. Hasil penelitian menunjukan nilai terbaik pada fekunditas sebesar 1191,67 ± 239,45 butir/200 g, daya tetas telur sebesar 80,93 ± 3,90%, kelulushidupan sebesar 81,55 ± 7,54%, laju pertumbuhan spesifik sebesar 7,26 ± 0,25, konversi pakan 0,54 ± 0,02 didapatkan pada perlakuan C, akan tetapi untuk diameter dan bobot telur, panjang dan bobot larva Kuning Telur, dan panjang dan bobot larva lepas kuning telur tidak berbeda nyata. Hasil tersebut menunjukan bahwa hibridisasi (perlakuan C) memberikan pengaruh yang nyata (P < 0,05) terhadap fekunditas, daya tetas telur, kelulushidupan, laju pertumbuhan spesifik, dan konversi pakan tetapi tidak berpengaruh terhadap diameter dan bobot telur, panjang dan bobot larva, dan panjang dan bobot larva lepas kuning telur. Kata kunci: Hibridisasi, Ikan Nila, Karakter Reproduksi, Performa Benih ABSTRACT This research aims to know the influence of hybridization tilapia fish Pandu F6 with nila Nilasa against the character of the reproduction and seed performance. This research was conducted with the experimental method using Random Design complete (RAL) with 4 treatments and three replicates. The treatments in this study: the treatment A (Pandu F6 F6 ♀ >< Pandu ♂), B (Nilasa ♀>< Nilasa ♂), C (Pandu F6 ♀>< Nilasa ♂), and D (Nilasa ♀ >< Pandu F6 ♂). The observed data covering fecundity, hatching rate, egg size, yolk sack larva length and weight, length and weight of the egg yolk off larvae, survival rate, specific growth rate, feed conversion rate, and water quality. The results showed the best value on the fecundity of 1191.67±239.45 eggs/200 g, Hatching rate 80.93 ± 3.90%, Survival rate of 81.55 ± 7.54%, specific growth rate of 7.26 ± 0.25, conversion fodder 0.54 ± 0.02 obtained at the treatment C, but for the diameter and weight of egg, larval length and weight TL, and the length and weight of the yolk egg off larvae for each treatment do not differ markedly. The results showed that hybridization (treatment C) gives a real influence (P < 0.05) of fecundity,hatching rate, survival rate but not with the egg size, larva weight and length, and the length and weight of larvae off yolk. Water quality on the spawning, hatching eggs and larvae found on the maintenance of a decent range for tilapia fish farming Keywords: hybridization, tilapia fish, character of the reproduction, seed performance

Download Full-text

The effect of specific growth rate on protein synthesis and secretion in the filamentous fungus Trichoderma reesei

Microbiology ◽

10.1099/mic.0.27458-0 ◽

2005 ◽

Vol 151 (1) ◽

pp. 135-143 ◽

Cited By ~ 67

Author(s):

Tiina M. Pakula ◽

Katri Salonen ◽

Jaana Uusitalo ◽

Merja Penttilä

Keyword(s):

Growth Rate ◽

Protein Synthesis ◽

Specific Growth Rate ◽

Trichoderma Reesei ◽

Growth Rates ◽

Protein Production ◽

Specific Growth ◽

Synthesis Rate ◽

Specific Rate ◽

Specific Growth Rates

Trichoderma reesei was cultivated in chemostat cultures on lactose-containing medium. The cultures were characterized for growth, consumption of the carbon source and protein production. Secreted proteins were produced most efficiently at low specific growth rates, 0·022–0·033 h−1, the highest specific rate of total protein production being 4·1 mg g−1 h−1 at the specific growth rate 0·031 h−1. At low specific growth rates, up to 29 % of the proteins produced were extracellular, in comparison to only 6–8 % at high specific growth rates, 0·045–0·066 h−1. To analyse protein synthesis and secretion in more detail, metabolic labelling of proteins was applied to analyse production of the major secreted protein, cellobiohydrolase I (CBHI, Cel7A). Intracellular and extracellular labelled CBHI was quantified and analysed for pI isoforms in two-dimensional gels, and the synthesis and secretion rates of the molecule were determined. Both the specific rates of CBHI synthesis and secretion were highest at low specific growth rates, the optimum being at 0·031 h−1. However, at low specific growth rates the secretion rate/synthesis rate ratio was significantly lower than that at high specific growth rates, indicating that at low growth rates the capacity of cells to transport the protein becomes limiting. In accordance with the high level of protein production and limitation in the secretory capacity, the transcript levels of the unfolded protein response (UPR) target genes pdi1 and bip1 as well as the gene encoding the UPR transcription factor hac1 were induced.

Download Full-text

Sporulation at minimum specific growth rate inAspergillus nidulans chemostat culture predicted using protein synthesis efficiency estimations

Journal of Basic Microbiology ◽

10.1002/(sici)1521-4028(199912)39:5/6<293::aid-jobm293>3.0.co;2-c ◽

1999 ◽

Vol 39 (5-6) ◽

pp. 293-298 ◽

Cited By ~ 5

Author(s):

M.E. Bushell ◽

A.T. Bull

Keyword(s):

Growth Rate ◽

Protein Synthesis ◽

Specific Growth Rate ◽

Specific Growth ◽

Chemostat Culture

Download Full-text

APLIKASI TEPUNG TESTIS SAPI YANG MENGANDUNG rGH DALAM PAKAN BUATAN TERHADAP RASIO JENIS KELAMIN, PERTUMBUHAN DAN KELULUSHIDUPAN IKAN NILA (Oreochromis niloticus)

Pena Akuatika Jurnal Ilmiah Perikanan dan Kelautan ◽

10.31941/penaakuatika.v17i2.656 ◽

2018 ◽

Vol 17 (2) ◽

Author(s):

Rachmat Nurul Huda ◽

Titik Sulistiowati ◽

Tristiana Yuniarti

Keyword(s):

Growth Rate ◽

Survival Rate ◽

Sex Ratio ◽

Specific Growth Rate ◽

Oreochromis Niloticus ◽

Specific Growth ◽

Sex Reversal ◽

Average Weight ◽

Fish Feed ◽

Male Sex

ABSTRAKBudidaya ikan nila (Oreochromis niloticus) terus dikembangkan hingga saat ini. Berbagai teknologi dalam bidang perikanan telah diterapkan, salah satunya yaitu sex reversal dalam hal ini jantanisasi. Ikan nila jantan lebih cepat pertumbuhannya dari pada ikan nila betina sehingga dapat meningkatkan hasil produksi. Tujuan penelitian ini yaitu untuk mengetahui pengaruh pemberian tepung testis sapi dan dosis terbaik tepung testis sapi dalam pakan yang mengandung rGH terhadap rasio jenis kelamin, pertumbuhan dan kelulushidupan ikan nila. Bahan uji yang digunakan adalah larva ikan nila dengan stadia larva yang berumur 7 hari setelah lepas kuning telur dan memiliki bobot rata-rata 0,07 gram/ekor. Penelitian ini menggunakan metode eksperimental dengan Rancangan Acak Lengkap 4 perlakuan dan 3 kali ulangan. Perlakuan dalam penelitian ini yaitu dengan menambahkan tepung testis sapi dengan dosis pada perlakuan A (0%/kg pakan), perlakuan B (3%/kg pakan), perlakuan C (6%/kg pakan) dan, perlakuan D (9%/kg pakan). Pakan pada setiap perlakuan dalam penelitian ini telah ditambahkan rGH dengan dosis 2 mg/kg pakan. Data yang diamati meliputi rasio jenis kelamin, laju pertumbuhan spesifik (SGR), dan tingkat kelulushidupan (SR) ikan nila. Hasil penelitian menunjukan bahwa perlakuan C (6% tepung testis sapi/kg pakan) memberikan hasil terbaik dimana nilai rasio jenis kelamin jantan sebesar 74,44±5,09% dan laju pertumbuhan spesifik sebesar 9,48±0,10% bobot/hari. Kesimpulan yang diperoleh dalam penelitian ini yaitu adanya pengaruh nyata terhadap rasio jenis kelamin dan tingkat pertumbuhan spesifik, namun tidak berpengaruh nyata terhadap tingkat kelulushidupan ikan nila dengan pemberian dosis tepung testis sapi terbaik adalah 6% tepung testis sapi/kg pakan.Kata kunci: Sex reversal; Tepung testis sapi; Pertumbuhan, rGH; NilaABSTRACTTilapia fish (Oreochromis niloticus)cultivation still continue to be developed until nowdays. Many fisheries technology had been applied, and one of them is sex reversal which is masculinization. Male tilapia fish grows faster than the female one, so this can boost the produce of the fish. This research aims to know the effect of cow testicle flour addition and determine the best dosage on the fish feed which contains rGH towards the male-sex ratio, growth, and survival rate of tilapia fish. The material which used in this research was tilapia fish (O. niloticus) which under the larvae phase 7 days post the separated egg yolk which have average weight of 0,07 grams/each. This research used experimental methods with Completely Randomized Design of 4 treatments and 3 times of repetitions. The treatments in this research was addition of different dosage of bull testicle which treatment A (0%/kg fish feed),treatment B (3%/kg fish feed), treatment C (6%/kg fish feed) and, treatment D (9%/kg fish feed). The fish feed in every treatment had been added with 2mg rGH/kg fish feed. Data observation during this research were male-sex ratio, specific growth rate (SGR), and survival rate (SR) of tilapia fish. Results of the research showed that treatment C (6%/kg fish feed) gave the best result as male-sex ratio by 74,44±5,09% and specific growth rate by 9,48±0,10% weight/day. Conclusion obtained from this research was: there was different result towards the male-sex ratio and specific growth rate, but there was no difference between the treatments towards the survival rate, the best bull testicle flour dosage was 6%/kg fish feed. Keywords: Sex reversal, Bull testicle flour, Growth, rGH, Tilapia

Download Full-text

GIFT Nile tilapia Oreochromis niloticus fed on diet containing probiotic

Jurnal Akuakultur Indonesia ◽

10.19027/jai.12.106-112 ◽

2015 ◽

Vol 12 (2) ◽

pp. 106

Author(s):

Petrus Hari Tjahja Soedibya

Keyword(s):

Growth Rate ◽

Body Weight ◽

Specific Growth Rate ◽

Oreochromis Niloticus ◽

Nile Tilapia ◽

Specific Growth ◽

Initial Body Weight ◽

Protein Retention ◽

Nile Tilapia Oreochromis Niloticus ◽

Meal Protein

ABSTRACT This research was conducted to evaluate the effect of the diet containing probiotic on Nile tilapia, Oreochromis niloticus. Fish with an initial body weight of 3.62±1.33 g were fed on diet supplemented with either 0%, 10%, 15%, 20% of probiotic. Results showed that fish fed on the diet supplemented with 15% of probiotic had the highest protein retention and specific growth rate, which were16.48±0.49% and 17.65±0.9%/day respectively. Keywords: probiotic, Azola meal, protein retention, specific growth rate ABSTRAK Penelitian ini bertujuan untuk mengevaluasi efek pemberian pakan mengandung probiotik pada ikan nila, Oreochromis niloticus. Ikan dengan bobot awal 3,62±1,33 g diberi pakan mengandung 0%, 10%, 15%, 20% probiotic. Hasil penelitian menunjukkan bahwa ikan yang diberi pakan mengandung 15% probiotik memiliki retensi protein dan laju pertumbuhan tertinggi masing-masing dengan nilai 16,48±0,49% dan 17,65±0,9%/ hari. Kata kunci: probiotik, tepung Azola, retensi protein, laju pertumbuhan harian

Download Full-text

Effect of dietary threonine on growth performance and muscle growth, protein synthesis and antioxidant-related signalling pathways of hybrid catfish Pelteobagrus vachelli♀ × Leiocassis longirostris♂

British Journal Of Nutrition ◽

10.1017/s0007114519002599 ◽

2019 ◽

Vol 123 (2) ◽

pp. 121-134 ◽

Cited By ~ 1

Author(s):

Ye Zhao ◽

Qin Jiang ◽

Xiao-Qiu Zhou ◽

Shang-Xiao Xu ◽

Lin Feng ◽

...

Keyword(s):

Growth Rate ◽

Protein Synthesis ◽

Specific Growth Rate ◽

Specific Growth ◽

Muscle Growth ◽

Signalling Pathways ◽

Mrna Levels ◽

Hybrid Catfish ◽

Leiocassis Longirostris ◽

Pelteobagrus Vachelli

AbstractThe experiment was conducted to investigate the effects of dietary threonine (Thr) on growth performance and muscle growth, protein synthesis and antioxidant-related signalling pathways of hybrid catfish Pelteobagrus vachelli♀ × Leiocassis longirostris♂. A total of 1200 fish (14·19 (se 0·13) g) were randomly distributed into six groups with four replicates each, fed six diets with graded level of Thr (9·5, 11·5, 13·5, 15·4, 17·4 and 19·3 g/kg diets) for 56 d. Results showed (P < 0·05) that dietary Thr (1) increased percentage weight gain, specific growth rate, feed efficiency and protein efficiency ratio; (2) up-regulated growth hormone (GH), insulin-like growth factor 1 (IGF-1), proliferating cell nuclear antigen, myogenic regulation factors (MyoD, Myf5, MyoG and Mrf4) and myosin heavy chain (MyHC) mRNA levels; (3) increased muscle protein content via regulating the protein kinase B/target of rapamycin signalling pathway and (4) decreased malondialdehyde and protein carbonyl contents, increased catalase, glutathione-S-transferase, glutathione reductase and GSH activities, up-regulated mRNA levels of antioxidant enzymes related to NFE2-related factor 2 and γ-glutamylcysteine ligase catalytic subunit. These results suggest that Thr has a potential role to improve muscle growth and protein synthesis, which might be due to the regulation of GH-IGF system, muscle growth-related gene, antioxidative capacity and protein synthesis-related signalling pathways. Based on the quadratic regression analysis of specific growth rate, the Thr requirement of hybrid catfish (14·19–25·77 g) was estimated to be 13·77 g/kg of the diet (33·40 g/kg of dietary protein).

Download Full-text

ANALISIS EFEKTIFITAS HORMON TIROKSIN TERHADAP PERTUMBUHAN BENIH IKAN NILA SALIN (Oreochromis niloticus)

Journal of Aquaculture and Environment ◽

10.35965/jae.v2i2.512 ◽

2020 ◽

Vol 2 (2) ◽

pp. 35-38

Author(s):

Petrus Matius Sroyer ◽

Hadijah Hadijah ◽

Sri Mulyani

Keyword(s):

Growth Rate ◽

Specific Growth Rate ◽

Analysis Of Variance ◽

Oreochromis Niloticus ◽

Specific Growth ◽

Anova Test ◽

Test Parameters ◽

Absolute Growth

Tujuan dari penelitian ini untuk mengetahui pengaruh hormon tiroksin terhadap pertumbuhan ikan nila salin dengan beberapa konsentrasi yang berbeda dan secara oral. Penelitian ini dilakukan secara eksperimental dengan 4 perlakuan yaitu penambahan hormone Tiroksin pada pakan 10 g/kg pakan pada P1, 15g/kg pada P2, 25 g/kg pada P3 dan tanpa pemberian hormone pada pakan untuk control ada P4. Data dianalisis dengan menggunakan analysis of variance (ANOVA). Parameter uji dalam penelitian ini meliputi Laju Pertumbuhan Spesifik dan Pertumbuhan Mutlak. Hasil penelitian menunjukkan laju pertumbuhan 3,01-4,73%/hari, pertumbuhan mutlak 103,78 - 159,04 gram, sehingga penggunaan hormone Tiroksin terbaik 25 gram/kg pakan. The purpose of this study was to determine the effect of the thyroxine hormone on the growth of salted tilapia with several different concentrations and it’s done orally. This study was carried out experimentally with 4 treatments, namely the addition of the hormone thyroxine 10g/kg feed at P1, 15g/kg feed at P2, 25g/kg feed at P3 and without addition of hormones to feed for control at P4. The data were analyzed using analysis of variance (ANOVA). Test parameters in this study include the Specific Growth Rate and Absolute Growth. The results showed a growth rate of 3.01-4.73%/day and absolute growth of 103.78-159.04 grams, so that the best use of thyroxine hormone was 25 g/kg feed.

Download Full-text

PENGARUH PENAMBAHAN TEPUNG JAHE EMPRIT(Zingiber officinale var. Amarum) PADA PAKAN TERHADAP PERTUMBUHAN DAN DAYA HIDUP IKAN NILA (Oreochromis niloticus)

Jurnal Perikanan Pantura (JPP) ◽

10.30587/jpp.v1i1.294 ◽

2018 ◽

Vol 1 (1) ◽

pp. 50

Author(s):

Ahmad Hadi Ripaki ◽

Farikhah Farikhah ◽

Andi Rahmad Rahim

Keyword(s):

Growth Rate ◽

Survival Rate ◽

Specific Growth Rate ◽

Oreochromis Niloticus ◽

Specific Growth ◽

Zingiber Officinale ◽

Conversion Ratio ◽

Feed Conversion Ratio ◽

Feed Conversion ◽

Absolute Weight

Tilapia (Oreochromis niloticus) is a type of fish that has high economic value.Cultivation is determined by two factors: fish disease and growth. Among the results ofimmunostimulant studies added in the diet can increase the nonspecific immune response as wellas increase the growth of fish, immunostimulant ingredients are one of ginger emprit (Zingiberofficinale var Amarum). This study aims to analyze the effect of the addition of ginger flouremprit to growth rate, survival rate and feed conversion ratio of tilapia fish. The research methodused was Completely Randomized Design (CRD) consisting of 4 with 3 replications, namely:treatment A = 5, B = 7.5, C = 10 g/kg of feed and control (no addition of ginger). The mainparameters observed were specific growth rate, absolute weight, survival rate and feedconversion ratio. The supporting parameters are water quality consisting of temperature, pH andDO. Analysis of the data using ANOVA if influenced by further Tukey test (p <0,05). Theresults showed that the best growth in this study was in treatment with a dose of 7.5 g/kg of feedwith an absolute weight of 5.40 ± 0.34 g, a specific growth rate of 2.41 ± 0.08%/day, survivalrate that is equal to 90 ± 0,0%, and feed conversion ratio of 3.6 ± 0.15 g.

Download Full-text

PENGARUH SUBTITUSI LIMBAH PERUT IKAN TONGKOL (Euthynnus affinis) PADA PAKAN TERHADAP PERTUMBUHAN DAN EFISIENSI PAKAN IKAN NILA (Oreochromis niloticus)

Journal of Aquaculture and Environment ◽

10.35965/jae.v2i2.272 ◽

2019 ◽

Vol 2 (2) ◽

pp. 43-49

Author(s):

Agoeng Karyanto ◽

Hadijah Hadijah ◽

Erni Indrawati

Keyword(s):

Growth Rate ◽

Specific Growth Rate ◽

Analysis Of Variance ◽

Oreochromis Niloticus ◽

Feed Efficiency ◽

Specific Growth ◽

Tuna Fish ◽

Fish Intestine ◽

Absolute Growth ◽

Euthynnus Affinis

Penelitian ini bertujuan untuk mengetahui pengaruh substitusi limbah perut ikan Tongkol (Euthynnus affinis) terhadap per-tumbuhan, efisiensi pakan dan konsentrasi yang optimal dalam meningkatkan pertumbuhan ikan Nila (Oreochromis niloticus) dan mengacu pada pemanfaatan bahan baku limbah usus ikan tongkol sebagai bahan alternatif pengganti bahan baku tepung ikan yang mahal. Penelitian ini merupakan jenis penelitian kuantitatif, menggunakan metode eksperimental yang terdiri atas 4 per-lakuan yaitu P0 (Protein 27%), P1 (Protein 20%), P2 (Protein 25%), dan P3 (Protein 30%). Data dianalisis dengan menggunakan analysis of variance (ANOVA). Parameter uji dalam penelitian ini meliputi Laju Pertumbuhan Spesifik, Pertumbuhan Mutlak, dan Efisiensi Pakan. Hasil penelitian menunjukkan rata-rata laju pertumbuhan spesifik benih ikan Nila (Oreochromis niloticus) berkisar antara 0.65-94%, pertumbuhan mutlak berkisar antara 2-3 gr, efisiensi pakan berkisar antara 4-5%, dan subtitusi tepung usus ikan tongkol (Euthynnus affinis) tidak berpengaruh nyata terhadap laju pertumbuhan spesifik (P>0,05), sedangkan per-tumbuhan mutlak dan efisiensi pakan berpengaruh nyata terhadap subtitusi tepung usus ikan Tongkol (Euthynnus affinis) (p<0,05). This study aims to determine the effect of substitution of Euthynnus affinis on growth, feed efficiency and optimal concentration in increasing the growth of tilapia (Oreochromis niloticus) and referring to the use of raw materials for tuna intestine waste as an alternative ingredient for the expensive fish flour starch. This study is quantitative research, using an experimental method consisting of 4 treatments, namely P0 (Protein 27%), P1 (Protein 20%), P2 (Protein 25%), and P3 (Protein 30%). Data were analyzed using analysis of variance (ANOVA). Test parameters in this study include Specific Growth Rate, Absolute Growth, and Feed Efficiency. The results showed that the average specific growth rate of tilapia seeds (Oreochromis niloticus) ranged from 0.65% to 0.94%, absolute growth ranged from 2 gr to 3 gr, feed efficiency ranged from 4% to 5%, and substitution of tuna fish intestine flour (Euthynnus affinis) did not significantly affect the specific growth rate (P> 0.05), while absolute growth and feed efficiency significantly affected the substitution of flour of the tuna fish intestine (Euthynnus affinis) (p <0.05).

Download Full-text