scholarly journals Pretreatment of Seed With Hydrogen Peroxide for Mitigating Salt Stress of Some Hungarian Wheat Landraces at Seedlings Stage

2021 ◽  
Vol 923 (1) ◽  
pp. 012062
Author(s):  
W. A. E. Abido ◽  
S. Dhurgham ◽  
K. Altai ◽  
L. Zsombik ◽  
Á. Hadházy ◽  
...  

Abstract Salt stress is a stringent problem limiting germination and productivity of wheat all over the world, seed pretreatment can effectively induce salt tolerance. The present experiment conducted to investigate the germination, seedling and chemical parameters response of seven Hungarian wheat Landraces (Szentesi, Nyirádi, Kiszombori, Háromfai, Tapiószelei, Nagykállói and Szajlai) to seed soaking at four levels of hydrogen peroxide (H2O2) i.e. control, 0.5,.0 and 1.5% subjected to five levels of salt stress (control, 3.0, 6.0, 9.0 and 2.0 dSm−1). Treatments were arranged in Factorial Experimental based on Completely Randomized Design (CRD) with four replications. Under non-saline and salt stress H2O2 soaking significantly influenced all parameters. Soaking at 1.5% resulted the highest germination ability, seedling parameters, relative water content (RWC%), tolerance index (TI%) and potassium (K+ ppm) as well proline level in parallel with the lowest sodium content. However, wheat landraces responded differently to the treatments. Háromfai landrace came in the first rank followed by Tapiószelei, Kiszombori, Szajlai, Nyirádi, Szentesi and Nagykállói landraces. Háromfai landrace resulted the highest values of germination parameters, RWC, TI, K+ and proline contents. At the same time, the maximum values of Na+ content was detected in reverse order. Increasing salinity stress levels from 3.0 to 12.0 dSm−1’ significantly decreased all studied character except Na+ which was increased as compared with control treatment. Therefore, pretreatment of seeds with 1.5 % of H2O2 increased proline and K+ in contrast to the Na+ content. These results suggest that H2O2 pretreatment helps to increase seedlings growth of Háromfai landrace under high salt stress compared to the other examined wheat landraces.

2019 ◽  
Vol 52 (1) ◽  
pp. 26-33
Author(s):  
M. Khoshkharam ◽  
W. Sun ◽  
Q. Cheng ◽  
M.H. Shahrajabian

Abstract Allelopathy is the detrimental effect of one crop on germination or development of a plant of another species. A factorial layout within completely randomized design with four replications was used to survey the influence of barley extract on corn seeds. Treatments included plant organs extract (leaf, stem, root and total), and different barley extract densities (Nosrat cultivar) includes four levels of 0%, 25%, 50% and 100%. The influence of barley extract was significant on coleoptile weight, radicle weight, radicle length and coleoptile length. Plant organs had meaningful effect on germination rate, germination percentage, coleoptile weight, radicle weight, radicle length and coleoptile length. Among all experimental characteristics, coleoptiles length was influenced by interaction between barley extract and plant organ. Although, the highest germination rate and germination percentage was related to 25% and 100% of barley extract density, the maximum coleoptile weight, radicle weight, radicle length and coleoptiles length was related to control treatment (0%). Leaf extract has obtained the higher values of germination rate, germination percentage, coleoptile weight, radicle weight, radicle length and coleoptile length. Interaction between control treatment (0% plant extract) and stem extract had obtained the highest coleoptiles weight, radicle weight, radicle length and coleoptile length. Hence, from the obtained results, it can be concluded that the extracts of barley may have allelopathic influence on germination and seedling growth of corn.


2017 ◽  
Vol 15 (2) ◽  
pp. 182-187
Author(s):  
D Das ◽  
MA Ali ◽  
TA Sarkar ◽  
MY Ali

The experiment was conducted at the Agronomy Laboratory of Agrotechnology Discipline, KhulnaUniversity, Khulnato determine the effect of salt stress on germination and seedling growth of indigenous Aman rice varieties during June, 2015 to December, 2015.  The salt tolerant landraces will be used as genetic resource for variety development program in future. The factorial experiment consists of two factors such as ten indigenous Aman rice varieties and four levels of salt solutions viz EC (dS m−1) of 5, 10 and 15 with control (distilled water). The experiment was laid out in Completely Randomized Design (CRD) with three replications. The interaction effects between variety and salinity on germination and seedling growth parameters of indigenous Aman rice varieties were varied significantly. It was found that germination capacity, energy, speed, seedling vigor index, root length and shoot length of rice were decreased gradually with increasing salinity. The germination energy and germination speed of most varieties were drastically reduced at 10 dS m−1 or higher salinity levels. The germination capacity of Hatibazor, Boushohagi, Shadagotal and Moinamoti were recorded 97.66 %, 99.33%, 98.0 % and 97.33%, respectively at 15 dS m−1 water salinity in laboratory condition. The results revealed that the indigenous Aman rice varieties named Boushohagi, Shadagotal, Hatibazor, Moinamoti, Motha were performed better over other varieties considering of germination and seedling growth parameters.J. Bangladesh Agril. Univ. 15(2): 182-187, December 2017


2014 ◽  
Vol 11 (1) ◽  
pp. 41-46 ◽  
Author(s):  
MM Hasan ◽  
MAH Chowdhury ◽  
BK Saha ◽  
MR Islam

A pot experiment was conducted in the net house of the Department of Agricultural Chemistry, Bangladesh Agricultural University, Mymensingh to evaluate the influence of phosphorus (P) and sulphur (S) on major nutrient contents and their uptake by brinjal (cv. BARI brinjal-8) during the period from October, 2011 to May, 2012. The experiment was laid out in a completely randomized design with 12 treatments and 3 replications using four levels of P (0, 30, 60 and 90 kg P ha-1) and three levels of S (0, 15 and 30 kg S ha-1) along with the basal doses of urea, muriate of potash, boric acid, zinc oxide, cowdung and poultry manure. The study revealed that major nutrient contents and their uptake were significantly influenced by P and S interactions. They had positive significant effects on major nutrient contents and their uptake. Application of P increased N, P, K, Ca, Mg and S contents and their uptake upto 60 kg ha-1 and over the dose the values were reduced or near to control treatment. Similarly, the gradual increases of major nutrient contents and their uptake were found in S application upto 30 kg ha-1. Among the treatments the combination of P @ 60 kg ha-1 and S @ 30 kg ha-1 showed the highest N, P, K, S, Ca and Mg contents and their uptake. The lowest values were found in control treatment. Results showed that an appropriate interaction of P and S increased major nutrient contents of brinjal. DOI: http://dx.doi.org/10.3329/jbau.v11i1.18206 J. Bangladesh Agril. Univ. 11(1): 41-46, 2013


2018 ◽  
Vol 20 (1) ◽  
pp. 19
Author(s):  
Subagiya Subagiya ◽  
Ato Sulistyo ◽  
Umi Nurchasanah

<p>Wheat <em>(Triticum aestivum)</em> is one of main food source with carbohydrates which is consumed by world people. The loss of wheat during storage is caused by pests attack. Flour beetle <em>(Tribolium castaneum)</em> is one of the main pests of wheat flour. Biological insectisidies is a solution to solve the attack of warehouse pests. The aim of research are to study the effectiveness of srikaya seeds on <em>T. castaneum</em> in wheat flour and to study the quality of wheat flour after the application srikaya seeds to <em>T. castaneum. </em>The research were be held in Pandan Rejo Village, Kebakkramat Sub-District, Karanganyar Regency on 105 asl altitude during September until October 2017. The research use Complete Randomized Design (CRD) with two factors of four repetitions. Dose treatment for Srikaya seeds consist of four levels of controls, 2.5 g/100 g wheat, 5 g/100 g wheat, and 10 g/100 g of wheat and the form of application consist of two kinds are 0.5 mm srikaya seed and 1 mm srikaya seed. Research variables which are observed were toxicity srikaya seed, imago mortality, moisture content, wheight shrinkage of wheat, color of wheat, and odor of wheat. The result show that LD50 of srikaya seed on a form 0.5 mm is 3.97 g/100 g wheat and form 1 mm is 4.70 g/100 g wheat. The application of the srikaya seed to minimize changes in quality wheat. The lowest shrinkage weight is 2.43 g and highest on the control of wheat color 5.805 g. control treatment from while to white murky scales (2). Scent control treatment of wheat does not become musty semlling scented (1).</p>


2016 ◽  
Vol 26 (2) ◽  
pp. 231-242 ◽  
Author(s):  
Mahdi Hamedi ◽  
Pooran Golkar ◽  
Ahmad Arzani

To evaluate the response of different genotypes of safflower (Carthamus tinctorius L.) to in vitro salt stress was conducted. Callus derived from leaflet, pedicel, hypocotyls, and adaxial and abaxial surfaces of the leaf were subjected to in vitro salt stress at 0, 100 and 200 mM of NaCl. The relative growth rate (RGR), callus growth rate (CGR), relative water content (RWC), tolerance index (TOL) and necrosis percentage were assessed. Results of analysis of variance indicated significant effects of salt stress, significant differences among genotypes for all traits and significant genotype × salt stress interaction for CGR, RWC and necrosis traits. The application of NaCl decreased RGR, CGR, RWC and TOL, significantly, while a significant increase observed across all the tested explants and genotypes for necrosis percentage data. An Iranian safflower genotype (K21) superior for RGR, RWC and TOL was the most salt tolerant genotype at the cellular level.Plant Tissue Cult. & Biotech. 26(2): 231-242, 2016 (December)


2017 ◽  
Vol 9 (11) ◽  
pp. 181 ◽  
Author(s):  
Francisco V. da S. Sá ◽  
Lauter S. Souto ◽  
Emanoela P. de Paiva ◽  
Erbia B. G. Araújo ◽  
Fernanda A. de Oliveira ◽  
...  

The objective was to evaluate growth and tolerance of bell pepper cultivars under salt stress in the seedling stage. The experiment was carried out in protected environment (greenhouse) at the Center of Sciences and Agrifood Technology (CCTA) of the Federal University of Campina Grande (UFCG), located in the municipality of Pombal, Paraíba, from August to September 2014. Five bell pepper cultivars (C1 - Dulce All Big; C2 - All Big; C3 - Italiano Amarelo Gigante; C4 - Rubi Gigante; C5 - Casca Dura Ikeda) were evaluated at five levels of irrigation water salinity (0.6 (control); 1.2; 1.8, 2.4 and 3.0 dS m-1), arranged in 5 × 5 factorial scheme, in a completely randomized design, with four replicates. Bell pepper cultivars were grown on trays for 20 days after sowing, period in which they were evaluated for emergence, growth, phytomass accumulation and tolerance index. The increase in irrigation water salinity reduced bell pepper emergence, growth and dry matter accumulation, and the cultivars C2 - All Big and C4 - Rubi Gigante were the most tolerant to salinity in the initial growth stage. Tolerance to salinity occurred in the following order: C2 - All Big > C4 - Rubi Gigante > C1 - Dulce All Big = C5 - Casca Dura Ikeda > C3 - Italiano Amarelo Gigante.


2016 ◽  
Vol 24 (1) ◽  
pp. 13-19 ◽  
Author(s):  
Abdolrahman Rahimian-Boogar ◽  
Hassan Salehi ◽  
Noshin Mir

Abstract Quality of cut flowers is an important issue at postharvest as well as an important factor contributing to marketing of and profitability from the tuberose. In this study, the effects of citric acid (CA) and hydrogen peroxide (H2O2) added to the vase water on postharvest quality of tuberose cut flowers were investigated. CA was applied in concentrations of 50, 100, 200, 400 mg·dm−3 and H2O2 in concentrations of 10, 20, 40 and 80 mg·dm−3 and distilled water as control treatment. Results showed that both compounds had significant positive effects on solution uptake, wilting and abscission of florets, relative water content, chlorophyll content, and vase life duration. The effects of 100 and 200 mg·dm−3 of CA and 20 and 40 mg·dm−3 of H2O2 proved to be more effective than other treatments. Both compounds increased the vase life of tuberose cut flowers and CA at concentrations 100 and 200 mg dm−3 and H2O2 at concentrations 20 and 40 mg dm−3 doubled this time up to 14-17 days.


F1000Research ◽  
2020 ◽  
Vol 9 ◽  
pp. 293
Author(s):  
Iskandar Putra ◽  
Irwan Effendi ◽  
Iesje Lukistyowati ◽  
Usman M. Tang ◽  
Muhammad Fauzi ◽  
...  

Background: High stocking density and intensive feeding in aquaculture systems lead to the accumulation of organic waste, which results in an increase in ammonia, nitrite, and nitrite concentrations in culture media. Biofloc is a potential technology to overcome this problem. The starter is a crucial carbon source for bacteria in the formation of biofloc. The objective of the present study aimed to explore the best starter of biofloc in a red tilapia Oreochromis niloticus culture system. Methods: A completely randomized design with four levels of treatment was used in this study. The tested starter was (A) control treatment, biofloc without starter, (B) biofloc with molasses starter, (C) biofloc with tapioca starter, and (D) biofloc with sucrose starter. The floc was cultured in 100-L tanks with a salinity of 17 ppt. The tanks were stocked with O. niloticus with a size of 3.71±0.11 cm at a stocking density of 30 fish per tank. The fish were fed on a commercial diet two times a day at satiation for 40 days. The ammonia, nitrite, and nitrite concentrations were measured for an interval of 8 days. Results: The study showed that the NH3-N range was 0.02–0.07 mg L−1 (mean, 0.03 ± 0.02 mg L−1), NO2-N range was 0.20–0.43 mg L−1 (mean, 0.25 ± 0.12 mg L−1), and NO3-N range was 0.90–3.20 mg L−1 (mean, 1.42 ± 1.19 mg L−1). Conclusion: Among the starters tested, molasses was found to be the best for biofloc in tilapia culture.


2021 ◽  
Vol 6 (3) ◽  
pp. 65654
Author(s):  
Mohammad Agus Salim ◽  
Luluk Setyaningsih ◽  
Imam Wahyudi ◽  
Sri Wilarso Budi R

Iron (Fe) is a micro essential needed by plants in small amounts and can be toxic when available in large quantities. This study aimed to evaluate how Fe exposure affects the growth of C. callothyrsus and L. leucocephala seedlings. This study used a completely randomized design with factorial, where the first factor consisted of two levels of seedlings (C. calothyrsus and L. leucocephala), and the second factor consisted of Fe concentration which consisted of 8 levels (0, 0.25, 0.5, 0.75, 1, 1.25, 1.5, and 1.75 mM). The results showed that treatment of seedlings species and concentration of Fe was able to significantly affect the growth parameters (height, root length, root dry weight, shoots, and plant dry weight) of seedlings. The control treatment (without Fe) showed the highest growth response compared to those treated with Fe exposure and an increase in Fe concentration was able to reduce all growth parameters in both seedlings. The 0.5 mM Fe concentration reduced all growth parameters of C. calothyrsus drastically, while in L. leucocephala, the Fe 0.75 concentration was able to decrease all growth parameters drastically. The tolerance index of both seedlings decreased with increasing Fe concentration. The rate of photosynthesis did not show a significant difference between treatments, meanwhile, it had a significant effect on chlorophyll affect chlorophyll (a, b, and total chlorophyll) and carotenoid content. The highest Fe content in C. calothyrsus seedlings was at a concentration of 1.5 mM (4.40%), while in L. leucocephala seedlings, the highest Fe content was at 1.7 mM (2.87%). 


2019 ◽  
Vol 13 (3) ◽  
pp. 325-335
Author(s):  
Alefsi David Sánchez-Reinoso ◽  
Yulieth Jiménez-Pulido ◽  
Jean Paul Martínez-Pérez ◽  
Carlos Salvador Pinilla ◽  
Gerhard Fischer

Climate change has resulted in an increasing frequency of the phenomenon “La Niña,” generating prolonged periods of waterlogging and low light. The objective of the present study was to evaluate the effects of two abiotic stresses: shading (65%) and waterlogging, and their interaction on fluorescence parameters of chlorophyll a in lulo (Solanum quitoense var. septentrionale) seedlings. A completely randomized design with a factorial arrangement was implemented. The first factor consisted of two levels of light (with and without shading). The second factor were four levels of duration of the waterlogging period (0, 3, 6 and 9 days), for a total of 8 treatments with three replicates. The response variables were recorded at 6, 12 and 18 days after the application of the waterlogging treatments began. Measurements of relative water content (RWC), electrolyte leakage, chlorophyll content and chlorophyll a fluorescence were recorded. The lulo plants appeared to be more susceptible to waterlogging than to shading, with a lower RWC when waterlogged 6 and 9 days, presenting damage at the level of photosystem II from day 3, causing a decrease in the chlorophyll content. The plants flooded under shading had a greater tolerance to this factor than those cultivated in full light. The techniques of quantification of the chlorophyll a fluorescence, especially the maximum quantum efficiency of the PSII, the effective photochemical quantum yield of PS II and the photochemical quenching were useful tools that characterized the lulo seedlings under stress conditions.


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