LncmiRSRN: identification and analysis of long non-coding RNA related miRNA sponge regulatory network in human cancer

AbstractLong non-coding RNA forkhead box D2 adjacent opposite strand RNA 1 (FOXD2-AS1) has emerged as a potential oncogene in several tumors. However, its biological function and potential regulatory mechanism in glioma have not been fully investigated to date. In the present study, RT-qPCR was conducted to detect the levels of FOXD2-AS1 and microRNA (miR)-506-5p, and western blot assays were performed to measure the expression of CDK2, cyclinE1, P21, matrix metalloproteinase (MMP)7, MMP9, N-cadherin, E-cadherin and vimentin in glioma cells. A luciferase reporter assay was performed to verify the direct targeting of miR-506-5p by FOXD2-AS1. Subsequently, cell viability was analyzed using the CCK-8 assay. Cell migration and invasion were analyzed using Transwell and wound healing assays, respectively. The results demonstrated that FOXD2-AS1 was significantly overexpressed in glioma cells, particularly in U251 cells. Knockdown of FOXD2-AS1 in glioma cells significantly inhibited cell proliferation, migration, invasion and epithelial–mesenchymal transition (EMT) and regulated the expression of CDK2, cyclinE1, P21, MMP7 and MMP9. Next, a possible mechanism for these results was explored, and it was observed that FOXD2-AS1 binds to and negatively regulates miR-506-5p, which is known to be a tumor-suppressor gene in certain human cancer types. Furthermore, overexpression of miR-506-5p significantly inhibited cell proliferation, migration, invasion and EMT, and these effects could be reversed by transfecting FOXD2-AS1 into the cells. In conclusion, our data suggested that FOXD2-AS1 contributed to glioma proliferation, metastasis and EMT via competitively binding to miR-506-5p. FOXD2-AS1 may be a promising target for therapy in patients with glioma.

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ADAMTS9-AS2: A functional long non-coding RNA in tumorigenesis

Current Pharmaceutical Design ◽

10.2174/1381612827666210325105106 ◽

2021 ◽

Vol 27 ◽

Author(s):

Wen Xu ◽

Bei Wang ◽

Yuxuan Cai ◽

Jinlan Chen ◽

Xing Lv ◽

...

Keyword(s):

Dna Methylation ◽

Signaling Pathways ◽

Therapeutic Target ◽

Molecular Mechanisms ◽

Human Cancer ◽

Migration Inhibition ◽

Cancer Proliferation ◽

Non Coding Rna ◽

Non Coding Rnas ◽

Long Non Coding Rna

Background: Long non-coding RNAs (lncRNA) have been identified as novel molecular regulators in cancers. LncRNA ADAMTS9-AS2 can mediate the occurrence and development of cancer through various ways such as regulating miRNAs, activating the classical signaling pathways in cancer, and so on, which have been studied by many scholars. In this review, we summarize the molecular mechanisms of ADAMTS9-AS2 in different human cancers. Methods: Through a systematic search of PubMed, lncRNA ADAMTS9-AS2 mediated molecular mechanisms in cancer are summarized inductively. Results: ADAMTS9-AS2 aberrantly expression in different cancers is closely related to cancer proliferation, invasion, migration, inhibition of apoptosis. The involvement of ADAMTS9-AS2 in DNA methylation, mediating PI3K / Akt / mTOR signaling pathways, regulating miRNAs and proteins, and such shows its significant potential as a therapeutic cancer target. Conclusion: LncRNA ADAMTS9-AS2 can become a promising biomolecular marker and a therapeutic target for human cancer.

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Neev, a novel long non-coding RNA, is expressed in chaetoblasts during regeneration of Eisenia fetida

10.1101/806661 ◽

2019 ◽

Author(s):

Surendra Singh Patel ◽

Sanyami Zunjarrao ◽

Beena Pillai

Keyword(s):

Eisenia Fetida ◽

Spatial Organization ◽

Cell Types ◽

Ventral Side ◽

Chitin Synthase ◽

Chitin Synthesis ◽

Mirna Sponge ◽

Non Coding Rna ◽

Temporal Expression Pattern ◽

Long Non Coding Rna

AbstractEisenia fetida, the common vermicomposting earthworm, shows robust regeneration of posterior segments removed by amputation. During the period of regeneration, the newly formed tissue initially contains only undifferentiated cells but subsequently differentiates into a variety of cell types including muscle, nerve and vasculature. Transcriptomics analysis, reported previously, provided a number of candidate non-coding RNAs that were induced during regeneration. We found that one such long non-coding RNA (lncRNA) is expressed in the skin, only at the base of newly formed chaetae. The spatial organization and precise arrangement of the regenerating chaetae and the cells expressing the lncRNA on the ventral side clearly support a model wherein the regenerating tissue contains a zone of growth and cell division at the tip and a zone of differentiation at the site of amputation. The temporal expression pattern of the lncRNA, christened Neev, closely resembled the pattern of chitin synthase genes, implicated in chaetae formation. We found that the lncRNA harbours 49 sites for binding a set of four miRNAs while the Chitin Synthase 8 mRNA comprises 478 sites. The over-representation of shared miRNA sites suggests that lncRNA Neev may act as a miRNA sponge to transiently de-repress chitin synthase 8 during formation of new chaetae in the regenerating segments of Eisenia fetida.Summary statementThe earthworm, Eisenia fetida, regenerates posterior segments following amputation. The transcriptome of the regenerating worm revealed a novel lncRNA, expressed only at the base of regenerating chaetae. We propose that this lncRNA is a miRNA sponge that modulates chitin synthesis.

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HOTAIR: An Oncogenic Long Non-Coding RNA in Human Cancer

Cellular Physiology and Biochemistry ◽

10.1159/000490131 ◽

2018 ◽

Vol 47 (3) ◽

pp. 893-913 ◽

Cited By ~ 87

Author(s):

Qing Tang ◽

Swei Sunny Hann

Keyword(s):

Drug Resistance ◽

Human Cancer ◽

Critical Role ◽

Stem Cell Differentiation ◽

Human Diseases ◽

Biological Functions ◽

Protein Coding ◽

Non Coding Rna ◽

And Function ◽

Long Non Coding Rna

Long non-coding RNAs (LncRNAs) represent a novel class of noncoding RNAs that are longer than 200 nucleotides without protein-coding potential and function as novel master regulators in various human diseases, including cancer. Accumulating evidence shows that lncRNAs are dysregulated and implicated in various aspects of cellular homeostasis, such as proliferation, apoptosis, mobility, invasion, metastasis, chromatin remodeling, gene transcription, and post-transcriptional processing. However, the mechanisms by which lncRNAs regulate various biological functions in human diseases have yet to be determined. HOX antisense intergenic RNA (HOTAIR) is a recently discovered lncRNA and plays a critical role in various areas of cancer, such as proliferation, survival, migration, drug resistance, and genomic stability. In this review, we briefly introduce the concept, identification, and biological functions of HOTAIR. We then describe the involvement of HOTAIR that has been associated with tumorigenesis, growth, invasion, cancer stem cell differentiation, metastasis, and drug resistance in cancer. We also discuss emerging insights into the role of HOTAIR as potential biomarkers and therapeutic targets for novel treatment paradigms in cancer.

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The functional roles of exosomes-derived long non-coding RNA in human cancer

Cancer Biology & Therapy ◽

10.1080/15384047.2018.1564562 ◽

2019 ◽

Vol 20 (5) ◽

pp. 583-592 ◽

Cited By ~ 8

Author(s):

Feiyu Chen ◽

Ning Wang ◽

Hor-Yue Tan ◽

Wei Guo ◽

Cheng Zhang ◽

...

Keyword(s):

Human Cancer ◽

Functional Roles ◽

Non Coding Rna ◽

Long Non Coding Rna

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The pseudogene-derived long non-coding RNA SFTA1P suppresses cell proliferation, migration, and invasion in gastric cancer

Bioscience Reports ◽

10.1042/bsr20171193 ◽

2018 ◽

Vol 38 (2) ◽

Cited By ~ 14

Author(s):

Hongwei Ma ◽

Tianshi Ma ◽

Miao Chen ◽

Zigui Zou ◽

Zhihong Zhang

Keyword(s):

Gastric Cancer ◽

Cell Proliferation ◽

Human Cancer ◽

Current Evidence ◽

Migration And Invasion ◽

Strongly Correlated ◽

Normal Tissues ◽

Advanced Tumor ◽

Non Coding Rna ◽

Long Non Coding Rna

Pseudogenes were once regarded as transcriptionally inactive and without specific molecular function. However, current evidence shows that pseudogene-derived long non-coding RNAs (lncRNAs) may be crucial regulators of human cancer development, including gastric cancer (GC). In the present study, we report that a pseudogene-derived lncRNA named surfactant associated 1, pseudogene (SFTA1P), which is 693-nt long, was significantly down-regulated in GC tissues compared with that in the adjacent normal tissues. In addition, decreased SFTA1P expression was strongly correlated with advanced tumor lymph node metastasis (TNM) stage, larger tumor size, lymphatic metastasis, and poor prognosis of patients with GC. Moreover, gain-of-function experiments revealed that the overexpression of SFTA1P inhibits cell proliferation, migration, and invasion, thus verifying the tumor inhibitory role of SFTA1P in GC. Furthermore, we investigated the potential action mechanism of SFTA1P. Our results showed that down-regulation of SFTA1P may be associated with decreased TP53 expression. In summary, our work suggests that the pseudogene-derived lncRNA SFTA1P functions as a tumor suppressor in GC and thus may act as a potential diagnostic and therapeutic target of GC.

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The Role of Long Non-Coding RNA NNT-AS1 in Neoplastic Disease

Cancers ◽

10.3390/cancers12113086 ◽

2020 ◽

Vol 12 (11) ◽

pp. 3086

Author(s):

Cong Zhou ◽

Shiwei Duan

Keyword(s):

Cancer Progression ◽

Cisplatin Resistance ◽

Malignant Tumors ◽

Human Cancer ◽

Neoplastic Disease ◽

Expression Levels ◽

Cancer Breast ◽

Non Coding Rna ◽

Non Coding Rnas ◽

Long Non Coding Rna

Studies have shown that non-coding RNAs (ncRNAs), especially long non-coding RNAs (lncRNAs), play an important regulatory role in the occurrence and development of human cancer. Nicotinamide nucleotide transhydrogenase-antisense 1 (NNT-AS1) is a newly-discovered cytoplasmic lncRNA. Many studies have shown that it has abnormally-high expression levels in malignant tumors, but there are also a few studies that have reported low expression levels of NNT-AS1 in gastric cancer, breast cancer, and ovarian cancer. At present, the regulatory mechanism of NNT-AS1 as a miRNA sponge, which may be an important reason affecting tumor cell proliferation, invasion, metastasis, and apoptosis is being studied in-depth. In addition, NNT-AS1 has been found to be related to cisplatin resistance. In this review, we summarize the abnormal expression of NNT-AS1 in a variety of neoplastic diseases and its diagnostic and prognostic value, and we explain the mechanism by which NNT-AS1 regulates cancer progression by competing with miRNAs. In addition, we also reveal the correlation between NNT-AS1 and cisplatin resistance and the potential clinical applications of NNT-AS1.

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Differential expression profiling of long non-coding RNA GAS5 and miR-126-3p in human cancer cells in response to sorafenib

Scientific Reports ◽

10.1038/s41598-019-45604-2 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 8

Author(s):

Teresa Faranda ◽

Ilaria Grossi ◽

Michele Manganelli ◽

Eleonora Marchina ◽

Gianluca Baiocchi ◽

...

Keyword(s):

Cancer Cells ◽

Differential Expression ◽

Expression Profiling ◽

Human Cancer ◽

Human Cancer Cells ◽

Non Coding Rna ◽

Long Non Coding Rna ◽

Differential Expression Profiling

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Long Non-Coding RNA MAGI2-AS3 is a New Player with a Tumor Suppressive Role in High Grade Serous Ovarian Carcinoma

Cancers ◽

10.3390/cancers11122008 ◽

2019 ◽

Vol 11 (12) ◽

pp. 2008 ◽

Cited By ~ 10

Author(s):

Priyanka Gokulnath ◽

Tiziana de Cristofaro ◽

Ichcha Manipur ◽

Tina Di Palma ◽

Amata Amy Soriano ◽

...

Keyword(s):

Ovarian Carcinoma ◽

High Grade ◽

Mirna Sponge ◽

Downstream Signaling ◽

Cellular Processes ◽

Serous Ovarian Carcinoma ◽

Non Coding Rna ◽

Important Player ◽

Non Coding Rnas ◽

Long Non Coding Rna

High-Grade Serous Ovarian Carcinoma (HGSC) is the most incidental and lethal subtype of epithelial ovarian cancer (EOC) with a high mortality rate of nearly 65%. Recent findings aimed at understanding the pathogenesis of HGSC have attributed its principal source as the Fallopian Tube (FT). To further comprehend the exact mechanism of carcinogenesis, which is still less known, we performed a transcriptome analysis comparing FT and HGSC. Our study aims at exploring new players involved in the development of HGSC from FT, along with their signaling network, and we chose to focus on non-coding RNAs. Non-coding RNAs (ncRNAs) are increasingly observed to be the major regulators of several cellular processes and could have key functions as biological markers, as well as even a therapeutic approach. The most physiologically relevant and significantly dysregulated non-coding RNAs were identified bioinformatically. After analyzing the trend in HGSC and other cancers, MAGI2-AS3 was observed to be an important player in EOC. We assessed its tumor-suppressive role in EOC by means of various assays. Further, we mapped its signaling pathway using its role as a miRNA sponge to predict the miRNAs binding to MAGI2AS3 and showed it experimentally. We conclude that MAGI2-AS3 acts as a tumor suppressor in EOC, specifically in HGSC by sponging miR-15-5p, miR-374a-5p and miR-374b-5p, and altering downstream signaling of certain mRNAs through a ceRNA network.

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