scholarly journals PSII-3 Carbohydrases enzymes improve the productive performance of grazing Nellore steers fed different nutritional plans during dry season

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 396-397
Author(s):  
Anderson P Acosta ◽  
Guilherme S Vasconcellos ◽  
Alexandre Perdigão ◽  
Victor V Valério de Carvalho ◽  
Tiago S Acedo ◽  
...  

Abstract The aim was to evaluate two nutritional plans with the addition of dosages of carbohydrases enzyme blend on productive performance of grazing Nellore bulls in dry season. A total of 102 Nellore bulls (BW = 294.10 ± 3.35 kg) were equally distributed in six paddocks (17 bulls/paddock) following a completely randomized design in a 2x3 factorial arrangement, being the factors different nutritional plans (2): mineral-proteic supplementation (PS; Fosbovi® Proteico 35) offered at 0,1% BW or mineral-protein-energetic supplementation (PES; Fosbovi® Proteico-Energético 25) offered at 0.3% BW; and levels of enzymatic carbohydrases blend (3):0,00 (control), 4,75 or 9,50 g/animal/day. The enzymatic blend was mainly composed by beta-glucanase and xylanase enzymes (Ronozyme® VP and Ronozyme® WX) and included in the supplements (both enzymes and supplements provided by DSM Nutritional Products Brasil S.A.). Supplement intake and animal weighting were carried out daily and individually by an automatic scale system installed in each paddock, thus, animal was considered the experimental unit. Data were analyzed using PROC MIXED of SAS and means were compared by polynomial regression. A quadratic effect was observed on enzyme level for ADG in both nutritional plans (P = 0.014), with intermediary dosages presenting the highest values of ADG for PS (0,283 vs. 0,188 kg/day) and PES (0.429 vs. 0.391 kg/day), over control respectively. Although no linear and quadratic enzyme effect was observed for final BW (FBW), animals supplemented with PES presented 20,06 kg higher FBW compared to PS (341,63 vs. 321,57 kg, P < 0.001). An increasing linear effect was observed for supplement intake (kg/day), for both PS and PES (P = 0.020). According the polynomial regression, it is recommended to include 4.67 g/day of carbohydrases blend in PS and 3.77 g/day in PES, being the carbohydrases blend a viable technology to grazing cattle. Moreover, mineral-protein-energetic supplementation can optimize Nellore bulls performance during dry season.

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 169-170
Author(s):  
Anderson Acosta ◽  
Alexandre Perdigão ◽  
Guilherme S Vasconcellos ◽  
Victor Valério de Carvalho ◽  
Tiago S Acedo ◽  
...  

Abstract The aim with this trial was to evaluate two nutritional plans with the addition of dosages of carbohydrases enzyme blend on productive performance of grazing Nellore bulls in rainy season. One hundred and two contemporary Nellore steers (BW = 294.10 ± 3.35 kg) were equally distributed in six paddocks (17 steers/paddock) following a completely randomized design in a 2x3 factorial arrangement, being the factors two nutritional plans: mineral-proteic supplementation (Fosbovi® Proteico 30; PS) offered at 0,1% BW or mineral-protein-energetic supplementation (Fosbovi® Proteico-Energético 25; PES) offered at 0.3% BW); and three levels of enzymatic carbohydrases blend (0, 4,75 or 9,50 g/animal/day). The enzymatic blend was mainly composed by beta-glucanase and xylanase enzymes (Ronozyme® VP and Ronozyme® WX); Both enzymes and supplemented were provided by DSM Nutritional Products Brasil S.A. Supplement intake and animal weighting were carried out daily and individually by an automatic scale system installed in each paddock. Data were analyzed using PROC MIXED of SAS and means were compared by polynomial regression. Animals that received PES had greater ADG (0.896 vs. 0.775 kg/day; P < 0.001) compared to those fed PS. Intermediary level (4.75 g/day) of carbohydrate enzymes also presented the highest ADG in PES compared to 0.0 level (0.941 vs. 0.896 kg/day; P = 0.006). Still, higher enzyme levels (9.5 g/day) presented greater ADG on PS, when compared to 0.0 level (0.852 vs. 0.775 kg/day, P = 0.006). PES animals presented greater intake, compared to PS (1.112 vs. 0.432 kg/day, P < 0.001). According to polynomial regression, it is recommended to include 4.75 g/day of these carbohydrases enzymes in PS and 4.39 g/day in PES for growing Nellore bulls in rainy season.


2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 466-467
Author(s):  
Anderson Acosta ◽  
Guilherme S Vasconcellos ◽  
Alexandre Perdigão ◽  
Victor Valério de Carvalho ◽  
Tiago S Acedo ◽  
...  

Abstract The aim with this trial was to evaluate two nutritional plans with the addition of dosages of carbohydrases enzyme blend on feeding behavior of grazing Nellore bulls in rainy season. One hundred and two contemporary Nellore steers (BW = 294.10 ± 3.35 kg) were equally distributed in six paddocks (17 steers/paddock) following a completely randomized design in a 2x3 factorial arrangement, being the factors two nutritional plans: mineral-proteic supplementation (Fosbovi® Proteico 35; PS) offered at 0,1% BW or mineral-protein-energetic supplementation (Fosbovi® Proteico-Energético 25; PES) offered at 0.3% BW); and three levels of enzymatic carbohydrases blend (0, 4,75 or 9,50 g/animal/day). The enzymatic blend was mainly composed by beta-glucanase and xylanase enzymes (Ronozyme® VP and Ronozyme® WX); Both enzymes and supplemented were provided by DSM Nutritional Products Brasil S.A. Feeding behavior data were collected daily and individually by an automatic scale/feeder system installed in the paddocks. Data were analyzed using PROC MIXED of SAS. Animals fed PES presented greater number of visits to the feed bunks (19.73 vs. 10.12 visits/day; P < 0.0001), to the water fountains (2.95 vs. 2.32 visits/day; P < 0.0001) and greater water intake (22.43 vs. 17.73 L/day; P < 0.0001) in comparison with animals fed PS treatments. Also, animals receiving PES spent more time taking supplement (1.332 vs. 545 seconds/visit; P < 0.0001) and water (203 vs. 153 seconds/visit; P < 0.0001) then PS animals did. No linear and quadratic effects were observed for feeding behavior when including carbohydrases enzymes to PES or PS supplement. PSE nutritional plan positively influence feeding behavior of Nellore bulls in dry season, increasing cattle feed bunk access and water intake in a daily basis.


2018 ◽  
Vol 40 (1) ◽  
pp. 38423
Author(s):  
Tatiane Souza dos Santos ◽  
Adriano Barbieri ◽  
Robert Guaracy Aparecido Cardoso Araujo ◽  
Gustavo Do Valle Polycarpo ◽  
Daniela Felipe Pinheiro ◽  
...  

The objective was to evaluate the performance, relative organ weight, morphometry, intestinal length and pancreatic enzyme activity of broilers receiving neonatal supplementation. A total of 900 Cobb 500 broiler chicks was housed in 30 boxes of 2.5 m². The treatments were divided in a completely randomized design with six replications, and consisted of five inclusion levels (0.0, 2.5, 5.0, 7.5 and 10 grams per bird). The supplement used was based of amino acids, vitamins and minerals, which was provided on the ration on the first day in the housing. Statistical analysis was performed using SAS (2008) and when significant broken down using a polynomial regression. Due to the inclusion of the neonatal supplement in the period from one to seven and one to 21 days of age, the feed intake presented a linear effect and the weight gain, a quadratic effect. The relative weights of the gizzard and liver showed a quadratic effect, and the inclusions of 4.18 and 3.41 grams/bird provided the lowest weights, respectively. The jejunum villi height decreased with supplementation as well as the activity of pancreatic lipase. It can be concluded that neonatal supplementation benefited the performance of birds up to 21 days of age.


Revista CERES ◽  
2011 ◽  
Vol 58 (5) ◽  
pp. 645-650 ◽  
Author(s):  
Gilberto Bernardo de Freitas ◽  
Márcio Sousa Rocha ◽  
Ricardo Henrique Silva Santos ◽  
Letícia Monteiro da Silva Freitas ◽  
Leandro de Almeida Resende

The objective of this work was to evaluate the productive performance of broccoli under different top-dressing organic fertilizations. The experiment was conducted under protected cultivation, in a completely randomized design with four replications, with two plants per experimental unit. Broccoli seedlings were produced in a commercial substrate in styrofoam trays. The seedlings were transplanted to plastic pots containing 10.0 L of substrate made up of subsoil and organic compost at the ratio of 3:1 (v/v), respectively, which is equivalent to about 20.0 t ha-1 of organic compost at planting. After seedling establishment, the top-dressing fertilization treatments were applied: gliricidia biomass associated or not with liquid biofertilizer of cattle manure to the soil and bokashi. Two control treatments were established: one with mineral fertilization recommended for the crop and the other without top-dressing fertilization. The broccoli production was evaluated (commercial standard). Plants that received mineral fertilizer were more productive, however, they were not significantly different (p>0.05), by Dunnet test, from the plants fertilized with 2.5 t ha-1 gliricidiabiomass (dry mass) associated with liquid biofertilizer (2.0 L m-2) applied to soil. Top-dressing fertilizations with only gliricidia, at 2.5 and 5.0 t ha-1 of biomass (dry mass), resulted in no significant increase in production of broccoli inflorescence. The use of bokashi in addition to gliricidia biomass and liquid biofertilizer reduced the efficiency of the fertilization compared with plants that received only gliricidia and liquid biofertilizer.


2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 449-450
Author(s):  
Anderson Acosta ◽  
Guilherme S Vasconcellos ◽  
Alexandre Perdigão ◽  
Victor Valério de Carvalho ◽  
Tiago S Acedo ◽  
...  

Abstract The aim with this trial was to evaluate two nutritional plans with the addition of carbohydrases enzyme blend (CEB) on ruminal fermentation of grazing Nellore bulls in dry season. Five rumen cannulated Nellore steers (BW = 350 kg) were used. Animals were randomly assigned to a treatment sequence in a 5×5 Latin square experiment with 15 d-periods (10 d to diet adaptation and 5 d for data collection), following a completely randomized design, being the treatments: mineral-proteic supplementation (Fosbovi® Proteico 35; PS) offered at 0,1% BW (0 or 4.75 g/animal/day of CEB) or mineral-protein-energetic supplementation (Fosbovi® Proteico-Energético 25; PES) offered at 0.3% BW (0, 4.75 or 9.50 g/animal/day of CEB). The CEB was mainly composed by beta-glucanase and xylanase enzymes (Ronozyme® VP and Ronozyme® WX). Both enzymes and supplements were provided by DSM Nutritional Products Brasil S.A. Rumen fluid samples were collected on the last day of each experimental period before (0), and 2, 4, 6, and 8 h after supplements were offered. Data were analyzed using PROC MIXED of SAS. Steers fed PSE had greater (P ≤ 0.041) rumen concentration of acetate, propionate, and butyrate, and lower (P = 0.028) acetate to propionate ratio in comparison with animals fed PS. Rumen acetate concentrations were 61.6 and 58.8 mmol/L and rumen propionate concentrations were 15.2 and 13.7 mmol/L for animals under the PSE and PS, respectively. A quadratic effect (P = 0.048) for carbohydrases enzyme blend was observed in NH3-N ruminal concentration of PES animals, where 4.75 g/day level presented the lower concentrations compared to control (24.13 vs. 32.20 mg/dL). Furthermore, rumen concentrations of acetate (P = 0.002) and propionate (P = 0.021) linearly increased with enzymes inclusion for PSE plan. PSE nutritional strategy positively influence ruminal fermentation of Nellore steers in dry season and its effects can be enhanced with CEB inclusion of 4.75 g/day.


2018 ◽  
Vol 39 (4) ◽  
pp. 1707
Author(s):  
Wiliam Parpinelli ◽  
Paulo Segatto Cella ◽  
Vaneila Daniele Lenhardt Savaris ◽  
Jomara Broch ◽  
Ricardo Vianna Nunes

The residual from the food industry has the potential to replace the traditional ingredients used in animal feed, and the dry brewery residue (DBR) may be an alternative for this purpose. This study aimed to evaluate the use of DBR in the diets of broiler from 22 to 42 days of age. Five hundred and forty-six male broilers were used, with an initial average weight of 865 ± 8 g. The animals were distributed in a completely randomized design, with six levels of DBR inclusion (0, 2, 4, 6, 8, 10%), seven replicates and thirteen animals per experimental unit (EU). Weight gain (WG), average feed intake (AFI) and feed:gain ratio were evaluated. At 42 days of age, blood samples were collected from two broilers of each EU, and one broiler per EU was slaughtered to investigate the intestinal development. DBR interposed the WG and promoted a linear effect in this variable from 22 to 42 days of age. This effect was not observed for AFI. The inclusion of DBR interfered with the serum cholesterol values, showing a quadratic influence at the maximum response for the inclusion of 4.96%. However, the blood parameters of triglycerides, uric acid, creatinine, aspartate aminotransferase, alanine aminotransferase and total protein remained unchanged, regardless of the level of dietary DBR. No changes in villus height and crypt depth were observed by intestinal morphometric evaluation. The inclusion of 10% DBR can be used in a poultry diet, without any damage to the performance, metabolism and development of the broilers.


2020 ◽  
Vol 40 (1) ◽  
pp. 133-143
Author(s):  
T. A. Amole ◽  
B. O. Oduguwa ◽  
O. S. Onifade ◽  
O. M. Arigbede ◽  
A. O. Jolaosho

Silages of sole maize and mixtures of maize-lablab were made at harvest in plastic bags. The experimental Unit consists of twelve cross-bred yearling bulls (White Fulani x N’dama) calves weighing 71-72kg and were randomly allotted to three dietary treatments: Grazing + sole maize silage, Grazing + mixtures of maize-lablab silage and Unsupplemented grazing as control. The experimental design was a completely randomized design and lasted 84days. Silage diets were given between 07:30 and 11:00 hours daily before grazing. Rumen fluid was collected at the end of the study to evaluate the co-efficient of ruminal fluid. The crude protein content in sole maize silage was half of that in mixture of maize-lablab silage. Inclusion of lablab in maize stover silage increased the mineral content as well as the lignin fraction. The mean pH value of sole maize silage (3.80) was less (P < 0.05) than 4.25 obtained with addition of lablab. The buffering capacity (5.37%) of sole maize silage significantly (P < 0.05) increased to 5.71% in mixture of maize-lablab silage. Lactic acid contents differed was influenced significantly (P < 0.05) with a mean value of 8.4% and 8.5% respectively in the sole maize silage and with lablab inclusion. Total volatile fatty acid (VFA) ranged from 56.7 μmoles/ml in calves without supplementation to 95.2 μmoles/ml in the calves fed mixture of maize-lablab silage. Mixture of Maize-lablab silage had the highest values for all the minerals determined in the feed materials while sole maize had the least. It was concluded from this trial, that forage legumes are relatively good sources of degradable nitrogen and fermentable energy, so their inclusion in the diet is likely to increase the rumen population of cellulolytic microbes and also proves that, mixture of maize-lablab silage could be used as supplement to enhance the growth and survival of calves during the dry season, when animal feeds are relatively scarce.


2021 ◽  
Vol 22 (3) ◽  
Author(s):  
Willan Orlando Caicedo ◽  
Felipe Norberto Alves Ferreira ◽  
Manuel Lázaro Pérez Quintana ◽  
Clarice Speridião Silva Neta ◽  
Walter Motta Ferreira

This study evaluated the effect of the inclusion of rejected taro tuber flour (RTTF) on the productive performance in commercial hybrid pigs (Landrace × Duroc × Pietrain) during their post-weaning period. The experiment was established under a completely randomized design, comprising four treatments (0, 10, 20, and 30% inclusion of RTTF in the diet), using a total of 60 piglets with 15 repetitions each. The average daily feed intake (ADFI), average daily gain (ADG), feed conversion (FC), final body weight (BW), presence of diarrheas, mortality, and production cost, were determined. RTTF showed a high dry matter content, nitrogen-free extracts, gross energy, aspartic acid, glutamic acid, potassium, iron, polyphenols, and antioxidant activity. RTTF inclusion in the diet of pigs in the entire period (30-58 d) did not cause diarrhea or deaths and showed a quadratic effect on BW (p < 0.001) and ADFI (p < 0.001) and produced a linear effect on ADG (p = 0.006), FC (p = 0.003) and production cost (p < 0.001). In conclusion, the inclusion of RTTF in the diet of pigs after weaning (30-58 d) ranging between 0 and 30 % showed no effect on BW and ADFI, with a slight decrease in ADG and FC and a linear effect on production cost reduction.  


Author(s):  
Lilian Dena dos Santos ◽  
Izabel Volkweis Zadinelo ◽  
Augusto Moesch ◽  
Robie Allan Bombardelli ◽  
Fabio Meurer

Abstract: The objective of this work was to evaluate the use of crude glycerol as an energy source to replace corn, in diets for Nile tilapia (Oreochromis niloticus) in the fattening stage. Two hundred Nile tilapia (190±4.5 g) were distributed in 20 tanks, in a completely randomized design. The treatments consisted of crude glycerol at 0, 60, 120, 180, and 240 g kg-1, to replace the dietary levels of corn, in order to maintain a constant digestible energy. Fish productive performance and the chemical composition of the fillet were evaluated. Histological analyses of the hepatopancreas, intestine, and visceral fat were also carried out. Crude glycerol had no effect on the performance parameters; however, it caused a quadratic reduction of visceral fat. There was an increase in the moisture and a decrease in the ether extract of the fillet. A linear effect on the blood glucose levels was verified in fish fed the diet without glycerol, and the peak occurred at 90 min. There were no differences for measurements of intestinal villi height and hepatocyte area. Crude glycerol can replace corn in diets for Nile tilapia in the fattening stage, without adversely affecting fish metabolism, productive performance, or survival.


2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 460-461
Author(s):  
Victor Valério de Carvalho ◽  
Alexandre Perdigão ◽  
Guilherme S F M Vasconcellos ◽  
Tiago S Acedo

Abstract Our objective was to evaluate the effects of mineral supplementation using the association of carboaminophosphochelates as mineral source plus Salinomycin on grazing beef cattle performance during growing phase. A total of 80 Nellore bulls (IBW = 292.72 ± 13.55 kg; Age, ~13 mo) were distributed in 8 paddocks (~2.7 hectares each) planted with Palisade Grass cv. Xaraés during the rainy to rainy-to-dry transition seasons (February to May). The experiment was a randomized complete block design (10 animals/paddock, 4 paddocks per treatment). The mineral supplementation was provided ad libitum and treatments were: Control; Mineral supplementation containing inorganic minerals, and TM+Salinomycin = Mineral supplementation containing carboaminophosphochelates (TM, Tortuga® Minerals) as minerals source (Cr, Se, Zn, Mn, S, Cu e Co) plus Salinomycin (~108 mg per animal/day). The supplement orts were removed and weighted daily to calculate supplement intake. The animals were weighed after a 14 h-fasting at the beginnig and end of the trial (84 days) to calculate weight gain. The paddock was considered the experimental unit and data were analyzed using PROC MIXED of SAS, whereby means were separated using the PDIFF statement (P &lt; 0.05). Supplement intake was similar between treatments (P = 0.13). Animals supplemented with TM+Salinomycin tended to have greater final BW (358 vs. 347, P = 0.07) and increased ADG in 22,3% (0.730 vs. 0.597 kg/d, P = 0.03) compared with animals fed Control. In conclusion, the combination of carboaminophosphochelates minerals plus salynomicin improves grazing beef cattle weight gain, with no deleterious effect on mineral supplement intake.


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