scholarly journals Effect of sole maize and maize –lablab silage on the ruminal volatile fatty acids (VFAS) of grazing calves in the dry season

2020 ◽  
Vol 40 (1) ◽  
pp. 133-143
Author(s):  
T. A. Amole ◽  
B. O. Oduguwa ◽  
O. S. Onifade ◽  
O. M. Arigbede ◽  
A. O. Jolaosho

Silages of sole maize and mixtures of maize-lablab were made at harvest in plastic bags. The experimental Unit consists of twelve cross-bred yearling bulls (White Fulani x N’dama) calves weighing 71-72kg and were randomly allotted to three dietary treatments: Grazing + sole maize silage, Grazing + mixtures of maize-lablab silage and Unsupplemented grazing as control. The experimental design was a completely randomized design and lasted 84days. Silage diets were given between 07:30 and 11:00 hours daily before grazing. Rumen fluid was collected at the end of the study to evaluate the co-efficient of ruminal fluid. The crude protein content in sole maize silage was half of that in mixture of maize-lablab silage. Inclusion of lablab in maize stover silage increased the mineral content as well as the lignin fraction. The mean pH value of sole maize silage (3.80) was less (P < 0.05) than 4.25 obtained with addition of lablab. The buffering capacity (5.37%) of sole maize silage significantly (P < 0.05) increased to 5.71% in mixture of maize-lablab silage. Lactic acid contents differed was influenced significantly (P < 0.05) with a mean value of 8.4% and 8.5% respectively in the sole maize silage and with lablab inclusion. Total volatile fatty acid (VFA) ranged from 56.7 μmoles/ml in calves without supplementation to 95.2 μmoles/ml in the calves fed mixture of maize-lablab silage. Mixture of Maize-lablab silage had the highest values for all the minerals determined in the feed materials while sole maize had the least. It was concluded from this trial, that forage legumes are relatively good sources of degradable nitrogen and fermentable energy, so their inclusion in the diet is likely to increase the rumen population of cellulolytic microbes and also proves that, mixture of maize-lablab silage could be used as supplement to enhance the growth and survival of calves during the dry season, when animal feeds are relatively scarce.

2018 ◽  
Vol 6 (2) ◽  
pp. 16-23
Author(s):  
Yopianus Anamila ◽  
Dyah Lestari Yulianti ◽  
Aju Tjatur Nugroho Krisnaningsih

ABSTRAK                    Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan tepung emping melinjo (Gnetum gnemon) pada pakan terhadap pH, kadar air, dan penyusutan daging ayam. Dalam percobaan dengan rancangan acak lengkap 4 perlakuan, 4 ulangan, satu unit eksperimen yang terdiri dari 5 ayam ayam yang dibagi secara acak. Keempat ulangan tersebut konsisten dengan empat perlakuan dari penelitian ini, masing-masing mengandung P0, P1, P2, dan P3 adalah 0% (kontrol), 5%, 10%, dan 15%. Melinjo emping flour (Gnetum gnemon) adalah sebagai pakan tambahan yang didapat dari pasaran. Variabel penelitian adalah: pH, kadar air, dan konsumsi daging ayam. Hasil penelitian menunjukkan bahwa kadar keripik leleh (Gnetum gnemon) tidak berpengaruh terhadap pH daging (P> 0,05) sedangkan pada kadar air dan penyusutan Daging ayam menunjukkan perbedaan pengaruh yang sangat signifikan (P <0,01). Nilai pH rata-rata pada perlakuan P0, P1, P2, dan P3 adalah 5,9; 5.9; 6.0 dan 5.9. Nilai rata-rata kadar air dalam perlakuan P0, P1, P2, dan P3 adalah 79,30; 84,90; 83.30, dan 83.40. Nilai rata-rata penyusutan daging ayam dalam perlakuan P0, P1, P2, dan P3 adalah 2,7; 4.2; 4,7 dan 4,7. Disimpulkan bahwa penambahan pati emping melinjo (Gnetum gnemon) berpengaruh pada kadar air dan penyusutan daging ayam. Namun, penggunaan tepung emping melinjo (Gnetum gnemon) tidak berkaryh pada pH daging ayam.    ABSTRACT                     This study aims to determine the effect of the use of emping melinjo flour (Gnetum gnemon) in a feed to pH, moisture content, and shrinkage of chicken meat. In an experiment with a completely randomized design of 4 treatments, 4 replications, one experimental unit consisting of 5 randomly divided chicken chickens. The four replications were consistent with four treatments from the study, each containing P0, P1, P2, and P3 were 0% (control), 5%, 10%, and 15%. Melinjo emping flour (Gnetum gnemon) is as an additional feed obtained from the market. The research variables were: pH, Water content, and Chicken Meat Meat Consumption. The results showed that the level of using melting chips (Gnetum gnemon) did not give effect to meat pH (P> 0,05) while in water content and shrinkage Chicken meat showed a very significant difference of influence (P <0.01). The mean pH value at treatment P0, P1, P2, and P3, was 5.9; 5.9; 6.0 and 5.9. The average value of water content in the treatment of P0, P1, P2, and P3 was 79.30; 84.90; 83.30, and 83.40. The mean value of shrinkage of chicken meat in treatment P0, P1, P2, and P3 was 2.7; 4.2; 4.7 and 4.7. It was concluded that the addition of emping melinjo starch (Gnetum gnemon) had an effect on water content and shrinkage of chicken meat. However, the use of emping melinjo flour (Gnetum gnemon) is not significant at the pH of chicken meat.


2013 ◽  
Vol 14 (2) ◽  
pp. 308-321
Author(s):  
Lina Maria Peñuela Sierra ◽  
Ivan Moreira ◽  
Antonio Claudio Furlan ◽  
Paulo Levi de Oliveira Carvalho ◽  
Ângela Rocio Poveda Parra ◽  
...  

Two experiments were carried out to determine the bioavailability of phosphorus in two spray-dried yeasts - sugarcane yeast (SCY), and sugarcane yeast + brewer's yeast (SCBY) - in starting pigs, by comparing different methods (Apparent Digestibility Coefficient of Phosphorus - ADCP; True Digestibility Coefficient of Phosphorus - TDCP; slope ratio; and standard curve). In experiment I, a digestibility assay were carried out using 30 cross breed pig with initial weigh of 22.69 ± 4.24kg, allotted in a completely randomized design. The mean ADCP and TDCP values were 62.68 and 64.15% for SCY and 77.01 and 79.33% for SCBY. ADCP and TDCP for SCBY were higher (P<0.05) than the values for SCY. In Experiment II, a growth test was conducted, 56 crossbred piglets, were utilized, with initial live weight of 15.11 ± 3.43kg, allotted in a completely randomized design, with seven treatments, four replications, and two pigs per experimental unit. The treatments consisted of a basal diet without supplementation with P and the same diet including supplementation with two levels of P (0.053% and 0.105%) from dicalcium phosphate, SCY and SCBY. The relative bioavailability mean value of 57.23% for SCY and 91.96% for SCBY, corresponding to 0.30% and 0.40% of available phosphorus, respectively.


2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 449-450
Author(s):  
Anderson Acosta ◽  
Guilherme S Vasconcellos ◽  
Alexandre Perdigão ◽  
Victor Valério de Carvalho ◽  
Tiago S Acedo ◽  
...  

Abstract The aim with this trial was to evaluate two nutritional plans with the addition of carbohydrases enzyme blend (CEB) on ruminal fermentation of grazing Nellore bulls in dry season. Five rumen cannulated Nellore steers (BW = 350 kg) were used. Animals were randomly assigned to a treatment sequence in a 5×5 Latin square experiment with 15 d-periods (10 d to diet adaptation and 5 d for data collection), following a completely randomized design, being the treatments: mineral-proteic supplementation (Fosbovi® Proteico 35; PS) offered at 0,1% BW (0 or 4.75 g/animal/day of CEB) or mineral-protein-energetic supplementation (Fosbovi® Proteico-Energético 25; PES) offered at 0.3% BW (0, 4.75 or 9.50 g/animal/day of CEB). The CEB was mainly composed by beta-glucanase and xylanase enzymes (Ronozyme® VP and Ronozyme® WX). Both enzymes and supplements were provided by DSM Nutritional Products Brasil S.A. Rumen fluid samples were collected on the last day of each experimental period before (0), and 2, 4, 6, and 8 h after supplements were offered. Data were analyzed using PROC MIXED of SAS. Steers fed PSE had greater (P ≤ 0.041) rumen concentration of acetate, propionate, and butyrate, and lower (P = 0.028) acetate to propionate ratio in comparison with animals fed PS. Rumen acetate concentrations were 61.6 and 58.8 mmol/L and rumen propionate concentrations were 15.2 and 13.7 mmol/L for animals under the PSE and PS, respectively. A quadratic effect (P = 0.048) for carbohydrases enzyme blend was observed in NH3-N ruminal concentration of PES animals, where 4.75 g/day level presented the lower concentrations compared to control (24.13 vs. 32.20 mg/dL). Furthermore, rumen concentrations of acetate (P = 0.002) and propionate (P = 0.021) linearly increased with enzymes inclusion for PSE plan. PSE nutritional strategy positively influence ruminal fermentation of Nellore steers in dry season and its effects can be enhanced with CEB inclusion of 4.75 g/day.


2010 ◽  
Vol 2010 ◽  
pp. 1-7 ◽  
Author(s):  
M. A. Abdl-Rahman ◽  
F. A. R. Sawiress ◽  
A. M. Abd El-Aty

The aim of the current study was to evaluate the effect of sodium lauryl sulfate-fumaric acid coupled addition onin vitromethangenesis and rumen fermentation. Evaluation was carried out usingin vitrogas production technique. Ruminal contents were collected from five steers immediately after slaughtering and used for preparation of inoculums of mixed rumen microorganisms. Rumen fluid was then mixed with the basal diet of steers and used to generate four treatments, negative control (no additives), sodium lauryl sulfate (SLS) treated, fumaric acid treated, and SLS-fumaric acid coupled addition treated. The results revealed that, relative to control, efficiency in reduction of methanogenesis was as follows: coupled addition > SLS-addition > fumaric acid addition. Both SLS-addition and SLS-fumaric acid coupled addition demonstrated a decremental effect on ammonia nitrogen (–), total short chain volatile fatty acids (SCVFAs) concentrations and the amount of substrate degraded, and an increment effect on microbial mass and microbial yield (). Nevertheless, fumaric acid did not alter any of the previously mentioned parameters but induced a decremental effect on –. Furthermore, both fumaric acid and SLS-fumaric acid coupled addition increased propionate at the expense of acetate and butyrate, while, defaunation increased acetate at the expense of propionate and butyrate. The pH value was decreased by all treatments relative to control, while, cellulase activity did not differ by different treatments. The current study can be promising strategies for suppressing ruminal methane emissions and improving ruminants feed efficiency.


2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 396-397
Author(s):  
Anderson P Acosta ◽  
Guilherme S Vasconcellos ◽  
Alexandre Perdigão ◽  
Victor V Valério de Carvalho ◽  
Tiago S Acedo ◽  
...  

Abstract The aim was to evaluate two nutritional plans with the addition of dosages of carbohydrases enzyme blend on productive performance of grazing Nellore bulls in dry season. A total of 102 Nellore bulls (BW = 294.10 ± 3.35 kg) were equally distributed in six paddocks (17 bulls/paddock) following a completely randomized design in a 2x3 factorial arrangement, being the factors different nutritional plans (2): mineral-proteic supplementation (PS; Fosbovi® Proteico 35) offered at 0,1% BW or mineral-protein-energetic supplementation (PES; Fosbovi® Proteico-Energético 25) offered at 0.3% BW; and levels of enzymatic carbohydrases blend (3):0,00 (control), 4,75 or 9,50 g/animal/day. The enzymatic blend was mainly composed by beta-glucanase and xylanase enzymes (Ronozyme® VP and Ronozyme® WX) and included in the supplements (both enzymes and supplements provided by DSM Nutritional Products Brasil S.A.). Supplement intake and animal weighting were carried out daily and individually by an automatic scale system installed in each paddock, thus, animal was considered the experimental unit. Data were analyzed using PROC MIXED of SAS and means were compared by polynomial regression. A quadratic effect was observed on enzyme level for ADG in both nutritional plans (P = 0.014), with intermediary dosages presenting the highest values of ADG for PS (0,283 vs. 0,188 kg/day) and PES (0.429 vs. 0.391 kg/day), over control respectively. Although no linear and quadratic enzyme effect was observed for final BW (FBW), animals supplemented with PES presented 20,06 kg higher FBW compared to PS (341,63 vs. 321,57 kg, P &lt; 0.001). An increasing linear effect was observed for supplement intake (kg/day), for both PS and PES (P = 0.020). According the polynomial regression, it is recommended to include 4.67 g/day of carbohydrases blend in PS and 3.77 g/day in PES, being the carbohydrases blend a viable technology to grazing cattle. Moreover, mineral-protein-energetic supplementation can optimize Nellore bulls performance during dry season.


Author(s):  
Petr Doležal

Twenty dairy cows were used in an experiment at which the influence of silages with different content of acids on the rumen fermentation was studied. Animals were divided into experimental and control group, each of them about 10 individuals. Feeding ratio consisted of maize silage (26 kg), alfalfa silage (13 kg), meadow hay (1 kg) and corn mixture (8.5 kg). The experimental group was fed an inoculated maize silage, with higher contents of fermentation acids. Intake of total acids per 1 kg live weigh was higher in the experimental group than control group (1.98 g/kg; 1.48 g/kg live weight, respectively). The rumen fluid was taken per orally 2-3 hours after feeding. Mean value of pH of rumen fluid was significantly (P<0.05) lower in the experimental group (5.8 ± 0.08) in comparison with control group (6.2 ± 0.06).The most significant increase (P<0.01) of VFA content (125 ± 5.2 mmol/l) and lactic acid content (4.8 ± 0.2 mmol/l) was observed in rumen fluid of experimental group. Significant differences (P<0.05) of propionic acid and butyric acid content were also found out between the groups. Nevertheless, acetic acid content was insignificant (P>0.05) lower in experimental group. A protozoa (infusoria) content was significantly (P<0.01) lower in the experimental group (175.76 ± 12.54 thousand/ml) as compared with the control group (288.1 ± 13.73 thousand/ml).


Author(s):  
Rittikeard Prachumchai ◽  
Anusorn Cherdthong ◽  
Metha Wanapat

The current work aimed to screen the ruminal cyanide-utilizing bacteria and evaluate the influence of fresh cassava root (FCR) and pellets containing high sulfur (PELFUR) on cyanide content, kinetics of gas, in vitro degradability, and ruminal fermentation. The experiment was conducted in a Completely randomized design (CRD) for a screening of cyanide-utilizing bacteria and the dietary treatments were the level of cyanide at 0, 150, 300, and 450 ppm. A 5 &times; 3 factorial arrangement in a Completely randomized design was used for in vitro study. Factor A was the level of FCR at 0, 260, 350, 440, and 530 g/kg of 0.5 g dry matter (DM) substrate, and factor B was the level of PELFUR at 0, 15, and 30 g/kg DM substrate. Adding different doses of cyanide significantly affected cyanide-utilizing rumen bacterial growth (p &lt; 0.05). Increasing the concentration of cyanide from 0 to 150 and 150 to 300 ppm, resulted in an increase in cyanide-utilizing rumen bacteria of 38.2% and 15.0%, respectively. Increasing the FCR level to more than 260 g/kg of 0.5 g substrate could increase cumulative gas production (p &lt; 0.05), whereas increasing doses of PELFUR from 15 to 30 g/kg increased the cumulative gas production when compared with that of 0 g/kg PELFUR (p &lt; 0.05). Cyanide concentration in rumen fluid decreased with PELFUR (p &lt; 0.05) supplementation. Degradability of in vitro dry matter and organic matter following incubation increased at 12 and 24 h due to PELFUR supplementation with FCR and increased additionally with 15 g/kg PELFUR (p &lt; 0.05) in 440 g/kg FCR. Proportions of the total volatile fatty acids, acetic acid (C2), propionic acid (C3), and butyric acid, as well as the ratio of C2 to C3 among supplementations with FCR (p &lt; 0.05) were significantly different. As the proportion of FCR increased to 530 g/kg of the substrate, the volume of C3 increased by 14.6%. This is the first finding of bacteria in the rumen capable of utilizing cyanide, and cyanide might function as a nitrogen source for bacterial cell synthesis. Inclusion of FCR of 530 g/kg with 30 g/kg PELFUR could increase the cumulative gas production, the bacterial population, the in vitro degradability, the proportion of C3, and the rate of the disappearance of cyanide.


2019 ◽  
Vol 7 (1) ◽  
pp. 1-12
Author(s):  
Kurniasih Kurniasih ◽  
Dade Jubaedah ◽  
Mochamad Syaifudin

Inland swamp water has low pH value 3-4, there fore is not suitable for striped catfish culture. Liming is one of the efforts to increase the pH of water. This research aims to increase the swamp water pH for survival rate and grow of catfish. This research used a completely randomized design four treatments and three replications with doses of 0,6 kg.m-2 equvalent to CaCO3 (P1), 0,9 kg.m-2 equvalent to CaCO3 (P2), 1,2 kg.m-2 equvalent to CaCO3 (P3) and 1,5 kg.m-2 equvalent to CaCO3 (P4). The parameters are water quality (temperature, pH, DO, ammonia, alkalinity, and hardness), growth and survival rate of striped catfish. The result showed that (P2) was the best treatment for increasing swamp water pH from 3,6 to 7,57 and soil pH from 3,5 to 7,60, survival rate 86,7%, absolute growth length 4,35 and absolute growth weight 8,30 g and feed efficiency 73,53%.


2017 ◽  
Vol 8 (2) ◽  
pp. 66
Author(s):  
Ahmad Teduh ◽  
Muarif Muarif ◽  
Rosmawati Rosmawati

Tujuan penelitian ini untuk mengetahui pertumbuhan dan kelangsungan hidup ikan hias Platydoras (Platidoras costatus) yang dipelihara dengan menggunakan teknologi bioflok. Penelitian ini menggunakan Rancangan Acak Lengkap dengan 2 perlakuan dan 5 ulangan.  Perlakuan dalam penelitian ini adalah: Perlakuan A (Tanpa Bioflok), B (Dengan Bioflok). Ikan uji yang digunakan adalah ikan hias Platydoras yang memiliki panjang 1,9–2,1 cm. Ikan dipelihara selama 40 hari dan diberi pakan 2 kali sehari. Parameter yang diamati meliputi laju pertumbuhan harian, kelangsungan hidup, pengukuran kualitas air dan kepadatan bakteri. Hasil penelitian menunjukan bahwa penambahan bioflok memberikan pengaruh yang berbeda nyata (P<0,05) terhadap laju pertumbuhan harian, dan tingkat kelangsungan hidup (SR). Kepadatan bakteri di awal penelitian (9.6x104 CFU/ml), pada akhir penelitian memiliki nilai rata-rata pada perlakuan tanpa bioflok berkisar 7.84x105 CFU/ml sedangkan nilai rata-rata pada perlakuan bioflok berkisar 4,3 x106 CFU/ml, Hasil indentifikasi pada awal penelitian terdapat 4 jenis bakteri yaitu Streptobacillus sp, Enterobacter sp, Kurthia sp, Aeromonas sp. pada akhir penelitian terdapat 2 jenis bakteri pada perlakuan tanpa bioflok (Streptobacillus sp dan Aeromonas sp) dan 2 jenis bakteri pada perlakuan bioflok (Streptobacillus sp dan Kurthia sp.). Kata Kunci: bioflok, pertumbuhan, platydoras, jenis bakteri.GROWTH AND GOING FISH LIFE PLATYDORAS (Platydoras costatus) FISH IN BIOFLOK TECHNOLOGYABSTRACT The purpose of this research is to know the growth and survival of Platydoras ornamental fish (Platidoras costatus) which is maintained by using biofloc technology. This study used Completely Randomized Design with 2 treatments and 5 replications. The treatments in this study were: Treatment A (Without Biofluok), B (With Bioflok). The test fish used are Platydoras ornamental fish that has a length of 1.9-2.1 cm. Fish kept for 40 days and fed 2 times a day. The parameters observed included daily growth rate, survival, water quality measurement and bacterial density. The results showed that the addition of bioflock gave significant different effect (P <0,05) to daily growth rate, and survival rate (SR). The density of the bacteria at the start of the study (9.6x104 CFU / ml), at the end of the study had an average value of treatment without biofloc ranging from 7.84x105 CFU / ml while the mean value of biofloc treatment ranged from 4.3 x106 CFU / ml, at the beginning of the study there were 4 types of bacteria: Streptobacillus sp, Enterobacter sp, Kurthia sp, Aeromonas sp. at the end of the study there were 2 types of bacteria on treatment without biofloc (Streptobacillus sp and Aeromonas sp) and 2 types of bacteria on biofloc treatment (Streptobacillus sp and Kurthia sp.).


2018 ◽  
pp. 691-698 ◽  
Author(s):  
Kerstin Maurus ◽  
Sharif Ahmed ◽  
Wiebke Getz ◽  
Marian Kazda

On demand biogas production is a great option to complement solar and wind power for the energy revolution. Alternatives like feedstock management are important in order to avoid expensive and complex adjustments for gas storage systems. The use of sugar beet silage (S) is a good option because it mainly contains carbohydrates that are easily degradable. Anaerobic digestion was performed for 63 days in four completely stirred tank reactors (CSTR) with different ratios of maize silage (M) and S. M given every hour was used as a base load for the fermentation and S was given two times a day every 12h. Biogas and methane production rates were measured every 5min in order to achieve data with high resolution. Also, pH value, VFA/TIC values and volatile fatty acids were measured during the experiment. The process remained stable in CSTR1 (M:S1:0), CSTR2 (M:S6:1) and CSTR3 (M:S3:1). Instabilities occurred in CSTR4 (M:S1:3) after an operation time of 33 days. Nevertheless, methane yields more than doubled for CSTR3 within 5min after the input of S. Use of sugar beet as a feedstock for biogas production is a further application for this agricultural commodity.


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