24 Effects of Spring versus Fall Calving on Fetal Growth, Vigor at Birth, and Neonatal Circulating Metabolites in Beef Calves

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 23-24
Author(s):  
Lindsey G Wichman ◽  
Colby A Redifer ◽  
Allison M Meyer
Keyword(s):  
Fetal Growth ◽  
Nutrient Availability ◽  
Total Protein ◽  
Fixed Effects ◽  
Single Point ◽  
Random Effect ◽  
Postnatal Life ◽  
Abdominal Girth ◽  
Urea Nitrogen ◽  
Calving Season

Abstract To determine the effect of season on perinatal nutrient availability, assessed through fetal growth, calf vigor, and neonatal circulating metabolites, multiparous and primiparous dams (age: 4.7 ± 2.4 yr; BCS: 5.3 ± 0.6) from 4 spring (2014–2017; n = 202) and 4 fall (2015, 2017–2019; n = 177) calving experiments were observed during parturition. Time to stand (spring: 67; fall: 104) was determined as minutes from birth to standing for 5 sec. After birth, calf BW and size (spring: 99; fall: 169; length, heart girth, abdominal girth, and cannon circumference) were recorded. Jugular blood samples were obtained from 63 spring and 89 fall calves at 0 (pre-suckling), 6, 12, 24, and 48 h postnatally. Data were analyzed either with the fixed effect of season (single point) or the fixed effects of season, hour, and their interaction with hour as a repeated effect (over time); calf sex was included when P < 0.25. Experiment was a random effect. Fall-born calves tended to have lighter (P = 0.09) BW and faster (P = 0.05) time to stand than spring-born calves. Season did not affect (P ≥ 0.18) other calf size measures. The season x hour interaction (P ≤ 0.07) affected circulating glucose, non-esterified fatty acids (NEFA), triglycerides, urea nitrogen, globulin, and total protein. Spring-born calves had greater (P ≤ 0.009) 0 h glucose, 0 and 6 h NEFA, and 0, 6, 12, and 48 h triglycerides than fall-born calves. Fall-born calves had greater (P = 0.03) total protein at 24 h and tended to have greater (P ≤ 0.10) total protein and urea nitrogen at 48 h and globulin at 24 h. Season affected albumin, which was greater (P = 0.003) in fall-born calves. These data suggest that calving season influences perinatal nutrient availability, which may impact the transition to postnatal life.

scholarly journals Systematic effects on activity, milk yield, milk flow rate and electrical conductivity

2002 ◽  
Vol 45 (3) ◽  
pp. 213-222 ◽  
Author(s):  
R. Firk ◽  
E. Stamer ◽  
W. Junge ◽  
J. Krieter
Keyword(s):  
Electrical Conductivity ◽  
Milk Yield ◽  
Flow Rate ◽  
Fixed Effects ◽  
Random Effect ◽  
Dairy Farm ◽  
Skewed Distribution ◽  
Milk Flow ◽  
Milking Frequency ◽  
Calving Season

Abstract. The traits activity, milk yield, milk flow rate and electrical conductivity were analysed in preparation for automatic oestrus detection. Collection of data was performed on a commercial dairy farm and milking took place in a rotary milking parlour. Between February and December 1998 1,090,031 observations from 2,422 Holstein Friesian cows were accumulated. Around 30% of cows were milked thrice daily. For each trait and each cow a daily value was calculated. The fixed effects test day, parity, calving season, milking frequency, week of lactation and the random effect cow were considered in statistical analyses. With increasing number of parity, activity decreased and milk yield, milk flow rate and electrical conductivity increased. The milking frequency had significant influence on all analysed traits and for the effect calving season no consistent trend was found. All traits showed characteristic patterns during lactation. Between test days high variations were found for the trait activity. The remaining traits showed a steady level except for small fluctuations. Repeatability was 27.4% for activity and between 70 and 78.7% for the milk parameters. The repeatabilities verified the collected field data having a satisfying structure for application in automatic oestrus detection. The repeatability of the trait activity indicated high differences between and within cows. The right skewed distribution indicated the activity as a promising trait for further analyses.

342 Effects of Maternal Nutrient Restriction During Late Gestation on Primiparous Dam Performance and Fetal Growth

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 190-190
Author(s):  
Colby A Redifer ◽  
Lindsey G Wichman ◽  
Allison M Meyer
Keyword(s):  
Fetal Growth ◽  
Fixed Effects ◽  
Late Gestation ◽  
Nutrient Restriction ◽  
Gestation Length ◽  
Net Energy ◽  
Abdominal Girth ◽  
Protein Requirements ◽  
Pre Treatment ◽  
Fall Calving

Abstract To determine the effects of late gestational nutrient restriction (NR) on heifer performance, fetal growth, and calving difficulty, single-sired fall-calving Hereford-SimAngus heifers (BW: 451 ± 28 [SD] kg; BCS: 5.4 ± 0.7) bred to a single sire were allocated by fetal sex and expected calving date to either 100% (control; CON; n = 12) or 70% (n = 13) of NASEM net energy and metabolizable protein requirements for maintenance, pregnancy, and growth. Beginning on d 160 of gestation, heifers were individually fed chopped sorghum sudan hay (1.74 Mcal ME, 6.66% CP, 72.0% NDF; DM basis) and based on individual intakes, supplemented to meet targeted nutritional planes. Dam BW, BCS, and backfat (BF) were determined pre-treatment, every 21 d (BW) or 42 d (BCS and BF) during treatments, and post-calving. At birth, calf BW and size (length, heart girth, abdominal girth, flank girth, cannon circumference, cannon length, shoulder height, and ribeye area) were measured. Data were analyzed with treatment, treatment initiation date, and calf sex (when P < 0.25) as fixed effects. Dam BW tended to be less (P = 0.09) and BCS was less (P = 0.04) at d 202 of gestation for NR dams. Dam BW, BCS, and BF were less (P ≤ 0.01) in NR dams for the remainder of gestation. Post-calving, NR dams weighed 64 kg less (P ≤ 0.01) than CON, with a BCS of 3.6 ± 0.1. Nutrient restriction did not affect (P ≥ 0.27) calf gestation length, BW, or size. Calf BW as % post-calving dam BW tended to be greater (P = 0.09) for NR calves. Fetal presentation was normal for all CON births, while 23.1% of NR dams had fetal malpresentation (P = 0.12). In the current study, NR dam BW and BCS were sacrificed during late gestation without altering fetal growth trajectory.

92 Maternal Nutrient Restriction of Primiparous Beef Heifers During Late Gestation Decreases Colostrum Yield and Reduces Calf Vigor

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 45-46
Author(s):  
Lindsey G Wichman ◽  
Colby A Redifer ◽  
Shelby L Davies ◽  
Allison M Meyer
Keyword(s):  
Repeated Measures ◽  
Fixed Effects ◽  
Single Point ◽  
Late Gestation ◽  
Nutrient Restriction ◽  
Postnatal Life ◽  
Net Energy ◽  
Beef Heifers ◽  
Protein Requirements ◽  
Calf Survival

Abstract We hypothesized that late gestational nutrient restriction (NR) would reduce colostrum yield and impair offspring transition to postnatal life. Primiparous, fall-calving crossbred beef heifers (BW: 451 ± 28 [SD] kg; BCS: 5.4 ± 0.7) were individually-fed either 100% (control; CON; n = 12) or 70% (n = 13) of NASEM net energy and metabolizable protein requirements for maintenance, pregnancy, and growth from d 160 of gestation to parturition. Delivery duration was determined as minutes from first fetal feet expulsion to birth. Calves were reared naturally by their dams and monitored for times from birth to first sternal recumbency, attempt to stand, and stand; vigor scores (1 = very weak, 5 = extremely vigorous) were assigned at 2, 5, 10, and 20 min of age. Total colostrum from one rear quarter was collected pre-suckling. Calf rectal temperatures were recorded at 0, 6, 12, 24, and 48 h postnatally. Data were analyzed with the fixed effects of treatment (single point) or treatment, hour, and their interaction (over time, using repeated measures). Date of calving was a fixed effect; calf sex was included when P < 0.25. Heifers fed CON had greater (P = 0.04) colostrum weight and volume than NR. Although gestational nutrition did not affect (P = 0.72) calf birth weight, calves born to CON heifers had faster (P = 0.02) times to stand and tended to have faster (P = 0.09) times to attempt to stand. Calves from CON heifers had greater (P = 0.05) 20 min vigor scores. Rectal temperatures at 0 h were greater (P = 0.02) in CON calves, but at 24 h were greater (P = 0.04) in NR calves. These data indicate that heifers nutrient restricted during late gestation have reduced colostrum yield and less vigorous calves, which may influence postnatal calf survival.

scholarly journals 165 Evaluation of Milk Urea Nitrogen Measurement Precision and Accuracy

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 134-134
Author(s):  
Kristan F Reed ◽  
Emma Wood ◽  
David M Barbano ◽  
Matilde Portnoy
Keyword(s):  
Milk Fat ◽  
Fixed Effects ◽  
Mixed Model ◽  
Linear Mixed Model ◽  
Random Effect ◽  
Nitrogen Efficiency ◽  
Urea Nitrogen ◽  
Milk Urea ◽  
Precision And Accuracy ◽  
Milk Urea Nitrogen

Abstract High levels of urea in blood, milk and urine have been linked to poor nitrogen efficiency, increased feed costs, poor reproductive performance and increased environmental impacts of dairy farming. Milk urea nitrogen (MUN) is a commonly used metric to manage herd nitrogen efficiency, with current recommendations for MUN to be between 8–14 mg/dL to maintain milk production and reduce nitrogen losses. However, a previous work suggests commercial analysis of MUN with mid-infrared spectroscopy (MIR) may not be precise enough to determine if a milk sample is within the recommended range. Thus, the objective of this study was to evaluate the precision and accuracy of milk testing lab MUN measurements. Four sets of bulk tank samples were sent to 3 commercial labs and one research lab for analysis by MIR. Samples were sent to commercial labs in duplicate and MUN was also assessed through an enzymatic assay. The Euclidean distance (ED) was calculated as a combined metric of precision and accuracy. The ED was not different between labs and ranged from 0.81–1.27. Repeatability (sr) and reproducibility (sR) were estimated for commercial labs and ranged from (0.297–0.469) and (0.555–0.791) respectively. Differences between individual sample MIR and enzymatic MUN were regressed on the centered enzymatic MUN in a linear mixed model that included a random effect of lab and fixed effects for milk protein and milk fat. Regression results indicate MIR analysis over-predicts MUN at low MUN concentrations and under predicts MUN at high MUN concentrations. Results suggest MIR analysis of MUN is more accurate around milk MUN, protein, and fat concentrations of ~13 mg/dl, 3.4% protein, and 4.2% fat. Further, the combined residual error and random effect of lab suggest the standard error of an MUN MIR measurement is ±1.8.

scholarly journals A random effects population dynamics model based on proportions-at-age and removal data for estimating total mortality

2012 ◽  
Vol 69 (11) ◽  
pp. 1881-1893 ◽  
Author(s):  
Verena M. Trenkel ◽  
Mark V. Bravington ◽  
Pascal Lorance
Keyword(s):  
Population Dynamics ◽  
Random Effects ◽  
Fixed Effects ◽  
Random Effect ◽  
Total Mortality ◽  
Estimation Bias ◽  
Effective Sample Size ◽  
Dynamics Model ◽  
Population Dynamics Model ◽  
Study Parameter

Catch curves are widely used to estimate total mortality for exploited marine populations. The usual population dynamics model assumes constant recruitment across years and constant total mortality. We extend this to include annual recruitment and annual total mortality. Recruitment is treated as an uncorrelated random effect, while total mortality is modelled by a random walk. Data requirements are minimal as only proportions-at-age and total catches are needed. We obtain the effective sample size for aggregated proportion-at-age data based on fitting Dirichlet-multinomial distributions to the raw sampling data. Parameter estimation is carried out by approximate likelihood. We use simulations to study parameter estimability and estimation bias of four model versions, including models treating mortality as fixed effects and misspecified models. All model versions were, in general, estimable, though for certain parameter values or replicate runs they were not. Relative estimation bias of final year total mortalities and depletion rates were lower for the proposed random effects model compared with the fixed effects version for total mortality. The model is demonstrated for the case of blue ling (Molva dypterygia) to the west of the British Isles for the period 1988 to 2011.

scholarly journals Phytobiotics with Adsorbent to Mitigate Toxicity of Multiple Mycotoxins on Health and Growth of Pigs

Toxins ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 442
Author(s):  
Debora Muratori Holanda ◽  
Young Ihn Kim ◽  
Wanpuech Parnsen ◽  
Sung Woo Kim
Keyword(s):  
Body Weight ◽  
Total Protein ◽  
Alanine Aminotransferase ◽  
Hepatic Function ◽  
Negative Effects ◽  
Serum Total Protein ◽  
Urea Nitrogen ◽  
Inflammatory Status ◽  
Tnf Α ◽  
Mycotoxin Adsorbent

Phytobiotics with a mycotoxin adsorbent were used to mitigate negative effects of multiple mycotoxins in diets fed to pigs. In experiment 1, 120 pigs (11.6 kg body weight; BW) were assigned to five treatments (three pigs/pen) and fed for 28 days. Treatments were CON (control), MTD (CON + 2.5 mg/kg of deoxynivalenol), DP (MTD + phytobiotics at 0.1%), and DPA1 and DPA2 (MTD + phytobiotics and adsorbent at 0.1% and 0.2%, respectively). In experiment 2, 96 pigs (28.5 kg BW) were assigned to four treatments (three pigs/pen) and fed for 26 days. Treatments were CON, MTAF (CON + 0.19 mg/kg of aflatoxin and 8 mg/kg of fumonisins), AFP (MTAF + phytobiotics at 0.1%), and AFPA (MTAF + phytobiotics and adsorbent at 0.1%). Growth performance was measured weekly, and blood was sampled at the end of study to measure hepatic function and inflammatory status (TNF-α). Data were analyzed using the MIXED procedure. In experiment 1, pigs fed MTD, DP, DPA1, and DPA2 had smaller (p < 0.05) BW than CON. Pigs fed DPA2 had greater (p < 0.05) BW than MTD. Pigs fed DP and DPA2 tended to have lower (p < 0.1) serum total protein than CON. Pigs fed MTD and DPA2 tended to have higher (p < 0.1) alanine aminotransferase than CON. Similarly, pigs fed MTD, DP, and DPA2 tended to have higher (p < 0.1) urea nitrogen/creatinine than CON. In experiment 2, pigs fed MTAF, AFP, and AFPA had smaller (p < 0.05) BW than CON. Pigs fed MTAF, AFP, and AFPA had smaller (p < 0.05) ADFI than CON. Pigs fed AFPA had higher (p < 0.05) aspartate aminotransferase than CON and MTAF. Pigs fed AFP and AFPA had higher (p < 0.05) alanine aminotransferase than CON. Pigs fed MTAF, AFP, and AFPA had lower (p < 0.05) urea nitrogen/creatinine than CON. Pigs fed AFPA had higher (p < 0.05) TNF-α than CON and MTAF. In conclusion, feeding an additional 2.5 mg/kg of deoxynivalenol or 0.19 mg/kg of aflatoxin with 8 mg/kg of fumonisins reduced the growth of pigs. Deoxynivalenol compromised the hepatic function of pigs. Phytobiotics with adsorbent could partly overcome the detrimental effects of mycotoxins.

Determining the Rate of Ergot-alkaloid Degradation in Toxic Endophyte-infected Tall Fescue Hay and Haylage During Long Term Storage

2021 ◽  
Vol 99 (Supplement_2) ◽  
pp. 22-22
Author(s):  
Charles A Zumbaugh ◽  
Susannah A Gonia ◽  
Kathryn M Payne ◽  
Thomas B Wilson
Keyword(s):  
Tall Fescue ◽  
Ergot Alkaloid ◽  
Fixed Effects ◽  
High Performance ◽  
Nutritive Value ◽  
Day Treatment ◽  
Random Effect ◽  
Storage Period ◽  
Neutral Detergent Fiber ◽  
Experimental Unit

Abstract The objectives of this experiment were to determine changes in the nutritive value and ergot alkaloid concentrations of endophyte-infected tall fescue hay and haylage during a 180-d storage period. Forage from a single field of Kentucky-31 tall fescue was cut for hay in late June and allowed to dry in the field. The dry matter (DM) of the windrow of cut forage was measured every 2 h after clipping. Forage was sampled from the windrow in 6 location blocks once forage DM reached target levels for haylage and hay treatments. Haylage and hay samples were taken when the DM of the windrow reached 50% and 80%, respectively. Seven subsamples of each treatment within block were chopped to 1.91 cm in length with a lettuce chopper and vacuum sealed in oxygen-excluding bags. Sample bags were stored indoors and opened at 30 d intervals over the 180-d storage period. Samples were analyzed for pH, nutritive value, and individual ergot alkaloid concentrations using high-performance liquid chromatography. Within each storage day, treatment within block was considered the experimental unit. Data were analyzed in SAS using the MIXED procedure with fixed effects of treatment, day, and the treatment by day interaction. Location block was considered a random effect. As expected, pH was decreased for haylage compared to hay at all time points (P &lt; 0.01) and DM was greater (P &lt; 0.01) for hay compared to haylage. Neutral detergent fiber values were greater (P &lt; 0.01) for hay compared to haylage and declined during storage (P &lt; 0.01). Total ergot alkaloid concentrations did not differ by treatment (P = 0.61), but ergovaline concentrations declined (P &lt; 0.01) during storage. Collectively, these results indicate minimal differences in nutritive value and ergot alkaloid concentrations between hay and haylage during storage, and that ergovaline concentrations decline during storage.

scholarly journals Effect of hay type on cecal and fecal microbiome and fermentation parameters in horses

2020 ◽  
Author(s):  
Rachel J Sorensen ◽  
James S Drouillard ◽  
Teresa L Douthit ◽  
Qinghong Ran ◽  
Douglas G Marthaler ◽  
...  
Keyword(s):  
Fixed Effects ◽  
Volatile Fatty Acids ◽  
Random Effect ◽  
Alpha Diversity ◽  
Sampling Location ◽  
Rrna Gene ◽  
Illumina Hiseq ◽  
Fecal Microbiome ◽  
Β Diversity ◽  
Fermentation Parameters

Abstract The effect of hay type on the microbiome of the equine gastrointestinal tract is relatively unexplored. Our objective was to characterize the cecal and fecal microbiome of mature horses consuming alfalfa or Smooth Bromegrass (brome) hay. Six cecally cannulated horses were used in a split plot design run as a crossover in 2 periods. Whole plot treatment was ad libitum access to brome or alfalfa hay fed over two 21-d acclimation periods with subplots of sampling location (cecum and rectum) and sampling hour. Each acclimation period was followed by a 24-h collection period where cecal and fecal samples were collected every 3 h for analysis of pH and volatile fatty acids (VFA). Fecal and cecal samples were pooled and sent to a commercial lab (MR DNA, Shallowater, TX) for amplification of the V4 region of the 16S rRNA gene and sequenced using Illumina HiSeq. Main effects of hay on VFA, pH, and taxonomic abundances were analyzed using the MIXED procedure of SAS 9.4 with fixed effects of hay, hour, location, period, all possible interactions and random effect of horse. Alpha and β diversity were analyzed using the R Dame package. Horses fed alfalfa had greater fecal than cecal pH (P ≤ 0.05) whereas horses fed brome had greater cecal than fecal pH (P ≤ 0.05). Regardless of hay type, total volatile fatty acid (VFA) concentrations were greater (P ≤ 0.05) in the cecum than in feces, and alfalfa resulted in greater (P ≤ 0.05) VFA concentrations than brome in both sampling locations. Alpha diversity was greater (P ≤ 0.05) in fecal compared to cecal samples. Microbial community structure within each sampling location and hay type differed from one another (P ≤ 0.05). Bacteroidetes were greater (P ≤ 0.05) in the cecum compared to the rectum, regardless of hay type. Firmicutes and Firmicutes:Bacteroidetes were greater (P ≤ 0.05) in the feces compared to cecal samples of alfalfa-fed horses. In all, fermentation parameters and bacterial abundances were impacted by hay type and sampling location in the hindgut.

Corporate Ownership Structure and Its Effect on Capital Structure: Evidence from BSE Listed Manufacturing Companies in India

2021 ◽  
pp. 227797522096830
Author(s):  
Palaniappan Gurusamy
Keyword(s):  
Capital Structure ◽  
Ownership Structure ◽  
Fixed Effects ◽  
Random Effect ◽  
Corporate Ownership ◽  
Manufacturing Companies ◽  
Hausman Test ◽  
Lm Test ◽  
Corporate Ownership Structure ◽  
The Capital Structure

The study aims to examine the relationship between corporate ownership structure and capital structure of BSE listed manufacturing firms in India. The study has included the sample of 357 companies which covers 16 major sectors during the period of 2006–2015. Considering the dynamic panel nature of the data relating to the capital structure and the ownership structure variables. The analysis undertakes a novel approach of examining the determinants both single equation and reduced equation models. In order to determine the most appropriate model, based on the F test, the Breusch Pagan LM test and finally the Hausman Test is conducted. The Hausman test result has been estimated by the fixed effect model is better than the other two models such as pooled OLS and random effect estimation. Based on the fixed effects results, size, risk and profitability have a highly significant relationship with leverage. Meanwhile, the growth opportunities and tangibility represent insignificant values. The study found that the explanatory variables of the promoters’ ownership and the institutional ownership have a negative impact on leverage, while the corporate ownership has a positive influence on the capital structure decision. The individual or public ownership has a negative and significantly related to the capital structure, whereas the effect of the foreign ownership inversely related to the firm’s leverage.

scholarly journals 251 Evaluation of Condensed Algal Residue Solubles as an Ingredient in Cattle Finishing Diets

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 137-138
Author(s):  
Calvin Gibbons ◽  
Andrea K Watson ◽  
Galen E Erickson ◽  
Bradley M Boyd ◽  
Levi J McPhillips ◽  
...  
Keyword(s):  
Great Plains ◽  
Fixed Effects ◽  
Block Design ◽  
Random Effect ◽  
Distillers Grains ◽  
Experimental Unit ◽  
Omega 3 ◽  
Finishing Diets ◽  
Randomized Block Design ◽  
Algae Oil

Abstract Algae oil production for Omega-3 fatty acid supplementation yields a byproduct called Condensed Algal Residue Solubles (CARS; 25.4% DM, 19.3% CP, 8.3% Fat, 9.96% Na on DM basis), de-oiled algae cells with residual fermentation substrates. This study evaluated the use of CARS in feedlot finishing diets. Crossbreed steers, (n=480) were blocked and stratified by initial body weight (BW) into 4 blocks, and assigned randomly to treatments. Treatments were designed as a 2 x 3 factorial with 3 inclusions of CARS (0, 2.5, 5% of diet DM) and 2 different base diets representing Northern and Southern Great Plains diets. The Southern diets contained steam flaked corn and dry distillers grains while the Northern diets had dry rolled and high moisture corn with wet distillers grains. CARS replaced corn in both diets. All blocks were harvested after 148 days on feed. Performance data were analyzed as a randomized block design with CARS inclusion, base diet, and interactions as fixed effects, BW block as a random effect and pen (n=48) as the experimental unit. Orthogonal contrasts were used to test linear and quadratic effects of CARS inclusion. There were no significant interactions between CARS inclusion and diet type (P ≥ 0.49). Main effects of CARS indicated positive quadratic responses for carcass adjusted ADG, G:F, 12th rib back fat, yield grade (P &lt; 0.01; increasing to 2.5% inclusion, decreasing at 5%) and hot carcass weight was both linear and quadratic (P ≤ 0.01 and P ≥ 0.06 respectively; 969, 977, 935 as CARS increased). Linear decrease in DMI, final adjusted BW and ribeye area (P ≤ 0.01) as CARS increased. Cattle fed the Southern diets had greater ADG and G:F compared to Northern diets (P &lt; 0.01). Including 2.5% CARS in the diet improved feed efficiency in both Northern and Southern based feedlot diets.

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