216 Effect of Dietary L-valine Supplementation on Growth Performance, Immune Response, and Microbial Metabolites in Weaned Pigs Raised in Varying Sanitary Conditions

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 113-114
Author(s):  
Bonjin Koo ◽  
Martin Nyachoti

Abstract This study investigated the effects of dietary L-valine supplementation and sanitary conditions on growth performance, immune response, and microbial metabolites in weaned pigs. Thirty-two weaned pigs (6.98 ± 0.47 kg) were randomly assigned to treatments in a 2 × 2 factorial arrangement based on dietary Val level and sanitary condition (clean or unclean). The pigs were fed either a basal diet containing the standard levels of Val suggested by NRC (2012) or the basal diet with an additional 0.1% of L-Val. A room designated as the clean room was washed weekly, whereas the designated unclean room was not washed throughout the experiment and 5 kg of manure from the nursery pig barn was spread on the pen floors on day 1. All data were analyzed using a mixed procedure of SAS, with the individual pen as the experimental unit. The pigs raised in unsanitary conditions exhibited a lower average daily gain and average daily feed intake and a higher incidence of diarrhea than those raised in sanitary conditions during the 14-day experimental period (P < 0.05). The pigs in the unclean group also had a lower concentration of butyrate in the jejunum and a higher concentration of ammonia-N in the colon than those in the clean group (P < 0.05). Dietary valine supplementation reduced plasma interleukin (IL)-1α and IL-1 receptor antagonist concentrations as well as isovalerate and ammonia-N concentrations in the colon (P < 0.05). Interactions between dietary Val supplementation and sanitary conditions were observed in the abundance of mRNA-encoding β-defensins 113, 125, and 129 (P < 0.05). In conclusion, dietary Val supplementation beneficially modulates inflammatory response and microbial metabolites regardless of sanitary condition, while transcriptional levels of β-defensins are regulated by dietary Val supplementation in a manner dependent on housing hygiene conditions.

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 90-90
Author(s):  
Kwangwook Kim ◽  
Yijie He ◽  
Cynthia Jinno ◽  
Seijoo Yang ◽  
Xunde Li ◽  
...  

Abstract The objective of this experiment was to investigate dietary supplementation of oligosaccharide-based polymer on growth performance, diarrhea, and fecal β-hemolytic coliforms of weaned pigs experimentally infected with a pathogenic F18 Escherichia coli (E. coli). Forty-eight pigs (7.23 ± 1.11 kg BW) were individually housed in disease containment rooms and randomly allotted to one of four treatments with 12 replicate pigs per treatment. The four dietary treatments were a nursery basal diet (control), and 3 additional diets supplemented with 50 mg/kg Mecadox (AGP), 10 or 20 mg/kg of oligosaccharide-based polymer. The experiment lasted 18 d [7 d before and 11 d after the first inoculation (d 0)]. The doses of F18 E. coli inoculum were 1010 cfu/3 mL oral dose daily for 3 days. Growth performance was measured on d -7 to 0 before inoculation, and d 0 to 5 and 5 to 11 post-inoculation (PI). Diarrhea score (DS; 1, normal, to 5, watery diarrhea) was daily recorded for each pig. Fecal samples were collected on d 2, 5, 8, and 11 PI to test the percentage of β-hemolytic coliforms in total coliforms. All data were analyzed by ANOVA using the PROC MIXED of SAS with pig as the experimental unit. Inclusion of oligosaccharide-based polymer linearly increased (P < 0.05) ADFI on d 0 to 5 PI, and feed efficiency on d 0 to 5 PI and d 5 to 11 PI (P = 0.07), compared with the control. Supplementation of AGP or oligosaccharide-based polymer reduced (P < 0.01) frequency of diarrhea of pigs from d 0 to 11 PI. No differences were observed in overall growth performance and percentage of fecal β-hemolytic coliforms on d 8 PI among pigs in AGP and oligosaccharide-based polymer treatments. In conclusion, supplementation of oligosaccharide-based polymer enhanced feed efficiency and reduced diarrhea of weaned pigs infected with a pathogenic E. coli.


2014 ◽  
Vol 14 (1) ◽  
pp. 101-115 ◽  
Author(s):  
Alessandro Agazzi ◽  
Erica Tirloni ◽  
Simone Stella ◽  
Serena Maroccolo ◽  
Barbara Ripamonti ◽  
...  

AbstractThe aim of this study was to evaluate the effects of the administration of a species-specific probiotic (Lactobacillus animalis SB310, Lactobacillus paracasei subsp. paracasei SB137 and Bacillus coagulans SB117 in a 30:35:35 ratio, respectively; 1.8 × 1010 CFU/g of powder) on gut microbial balance, immune response and growth performance of Holstein female calves during the first month of life. Twenty-two calves were divided into two experimental groups from 2 to 28 days of life: control (C), fed with milk replacer and concentrate as a basal diet, and treatment (T), fed C diet plus 1 g/calf/day of probiotic powder for the first month of age. Faecal and blood samples were individually collected and analysed weekly. Individual faecal score was recorded daily and general health score was calculated at the end of the trial. Cell-mediated immune response was evaluated by skin test at 7 and 28 days of life. Milk replacer and concentrate intake were recorded daily, while body weight and biometrical parameters were recorded at 2, 8, 14, 21 and 28 days of life, thus average daily gain and feed conversion rate were calculated. During the first week of treatment, lower blood eosinophil percentage (0.05% vs. 0.22%; P≤0.01) was found in T group, while basophils were higher in T than C group at the end of the trial (0.21% vs. 0.16%; P≤0.05). Higher faecal lactic acid bacteria (LAB)/E. coli ratio on day 28 of life (3.73 log CFU/g vs. 2.02 log CFU/g; P≤0.05) and lower incidence of diarrhoea were found in the treated group (63.30% vs. 70.71%; P=0.05). Body weight (48.92 kg vs. 46.92 kg; P≤0.05), total concentrate intake (14.77 kg vs. 12.56 kg on dry matter basis; P≤0.05), and heart girth (81.16 cm vs. 78.49 cm; P≤0.05) were significantly higher in T group. The administration of the probiotic during the first month of life improved gut microbiota and increased the growth performance and some biometric parameters of calves.


Author(s):  
Danung Nur Adli ◽  
Osfar Sjofjan

The aim of this study was to assess the effects of dietary probiotic enhanced liquid acidifier combined with mannan-rich fraction on growth performance, nutrients digestibility in growing pigs. Thirty [(Duroc×Yorkshire)×Landrace] pigs with the average initial BW of 36.75±1.57 kg were allocated into three treatments by a randomized complete block design. There were five pens per treatment with six pigs per pen. Dietary treatments include: 1) CON (basal diet); 2) T1 (basal diet+probiotic 0.1%) and 3) T2 (basal diet+probiotic+mannan rich fraction 0.2%). the data were analyzed as a randomized complete block design using of SAS University Version 4.0. The model included the effects of block (replication) and treatment. Pen served as the experimental unit. During the entire experimental period of 6 weeks, results showed that addition of complex probiotic at the level of 0.2% to diet increased ADG significantly (p<0.05). Also, digestibility of DM and N tended to increase. To sum up, results in this experiment indicated that dietary [(probiotik×acidifier)×mannan-rich-fraction]  supplementation had a positive effect on growing pigs performance and nutrient digestibility


2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 84-85
Author(s):  
Jinyoung Lee ◽  
J Caroline González-Vega ◽  
John K Htoo ◽  
Chengbo Yang ◽  
Martin Nyachoti

Abstract The objective of this experiment was to determine the effects of dietary crude protein (CP) content and crystalline amino acids (CAA) supplementation patterns on the growth performance of weaned pigs under clean (CSC) or unclean sanitary conditions (USC). One hundred forty-four piglets (6.35 ± 0.63 kg BW) were housed under CSC or USC for 3 wk and assigned to 1 of 3 diets: a high CP (HCP; 21%) and two low CP (LCP; 18%) diets supplemented with 10 crystalline indispensable amino acids (IAA) to meet all IAA requirements or only 6 IAA (Lys, Met, Thr, Trp, Val, and Ile) to meet IAA requirements except Leu, His, and Phe. Each treatment had 8 replicates (3 pigs per pen) per sanitary condition. The CSC room was cleaned and washed weekly. For the USC room, sow manure was spread and remained unwashed. Data were analyzed using the MIXED procedure of SAS with orthogonal polynomial contrasts. Pigs raised under USC had reduced (P &lt; 0.05) ADG and G:F in wk 2, but overall, no difference was found between sanitary conditions due to contrary results in wk 3. Overall, ADG and ADFI were not affected but G:F tended (P &lt; 0.10) to be lower for USC pigs. Also, G:F did not differ between HCP (0.79) and LCP (0.81) under CSC, however, LCP interactively decreased (P &lt; 0.05) G:F to 0.75 compared to 0.83 in HCP under USC. The CAA supplementation patterns did not influence growth except reduced (P &lt; 0.05) ADFI in wk 3. Pigs fed the HCP diet had higher (P &lt; 0.05) fecal scores throughout the experiment than those fed LCP diets under both sanitary conditions. In conclusion, overall growth performance did not differ between HCP and LCP under CSC, but LCP diets reduced G:F under USC. The fecal score decreased in LCP diets regardless of sanitary conditions.


2008 ◽  
Vol 88 (2) ◽  
pp. 271-281 ◽  
Author(s):  
E. A. Jeaurond ◽  
M. Rademacher ◽  
J. R. Pluske ◽  
C. H. Zhu ◽  
C. F. M. de Lange

Feeding fermentable carbohydrates (FC) to weanling pigs may reduce the negative impact of proteolytic fermentation on gastrointestinal health and function. A total of 144 newly weaned pigs [6.23 kg body weight (BW); six pens per treatment; six pigs per pen) were used to determine the interactive effects of feeding additional fermentable protein (FP) and FC on growth performance, gastrointestinal function and intestinal health. Dietary treatments, based on a 2 × 2 factorial arrangement, were: (1) basal diet (control); (2) control + 10% poultry meal (PM) as FP source; (3) control + 5% beet pulp (BP) as FC source; and (4) control + 10% PM and 5% BP. Diets were formulated to be similar in digestible energy (DE) and digestible amino acid contents. In general, no interactive effects of FC and FP were observed (P > 0.10). During the 3-wk post-weaning period, feeding FP reduced average daily gain (ADG) (242 vs. 269 g d-1; P < 0.05), while FC increased ADG (269 vs. 243 g d-1; P < 0.05). Overall, feed intake did not differ between treatments (P > 0.10). On days 14 and 28 post-weaning, Clostridia spp. counts in colon contents, counts of white cells and segmented neutrophils in blood were lowered (P < 0.05) by feeding FC. Blood urea nitrogen was increased by feeding FP (9.5 vs. 6.5 mg dL-1; P < 0.05), while ammonia concentration in colon contents was lowered by FC (154 vs. 193 µg mL-1) (P = 0.06). Among biogenic amines, levels of tyramine (140 vs. 304 nmol g-1 DM) and spermidine (174 vs. 219 nmol g-1 DM) in colon contents were lowered (P < 0.05) by FC. Acetic, proprionic and butyric acid contents in colon contents were increased by feeding FC, while valeric and caproic acid content decreased by feeding FP (P < 0.05). Feeding FC and FP had no effect (P > 0.10) on colon histology, pH, fecal consistency score and organ weights. Results suggest that FP and FC have independent effects on newly weaned pigs, while effects appear partly related to changes in gut microbiota. Key words: Enteric fermentation, fiber, gastrointestinal function, protein, pigs


2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 43-44
Author(s):  
Mariana Fontana Westphalen ◽  
Joonpyo Oh ◽  
Alexander N Hristov ◽  
Tara Felix

Abstract Objectives were to determine effects of rumen-protected Capsicum (RPC) on growth performance and immune response in growing beef cattle. Cattle were stratified by sex (steers = 24; heifers = 12) and body weight (BW; heavy or light) and randomly assigned to treatments: Control (no additive), or RPC (15 mg/kg of dry matter intake (DMI)). Cattle were transitioned over 21 days to a final diet of 80% grain mix and 20% corn silage. Cattle were weighed on test (d0 and 1), at end of adaptation (d21), at midpoint (d48 and 49), and off test (d98 and 99). Blood samples were collected on days 0, 21, and 98 for analysis of parameters related to immune function and energy status. Health treatments were recorded. Performance and blood variables (as repeated measures) were analyzed using the MIXED procedure of SAS. Pen was the experimental unit. Health data were analyzed using the GLIMMIX procedure; animal was the experimental unit. There was no interaction (P &gt; 0.10) of treatment and sampling day, nor main effect of treatment (P &gt; 0.10), for any blood parameters measured. There were no treatment effects (P &gt; 0.10) on DMI or feed efficiency. From d0 to 21, average daily gain (ADG) tended to be greater (P = 0.13) for animals fed RPC and was 22.9% greater (P = 0.10) for animals fed RPC from d22 to 49. While only 14% of the heavy animals were treated two or more times, 66% of light animals were treated two or more times (P &lt; 0.01), of which 77% were fed Control. Supplementing RPC improved early feedlot ADG. Although there were no changes in blood parameters, ADG improvement may be related to health status.


Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 2105
Author(s):  
Amirul Faiz Mohd Azmi ◽  
Hafandi Ahmad ◽  
Norhariani Mohd Nor ◽  
Goh Yong Meng ◽  
Mohd Zamri Saad ◽  
...  

This study investigates the effects of supplementation of the basal diet with concentrate and rumen bypass fat on the dry matter intake (DMI), growth performance, blood metabolites and hormonal changes, and the feeding cost of feedlot water buffaloes. Thirty-six healthy, three- to four-month-old male Murrah crossbred (n = 18) and Swamp (n = 18) buffaloes with a similar average initial body weight of 98.64 ± 1.93 kg were each randomly allocated into three dietary experimental groups. Buffaloes were fed with Diet A, which consisted of 100% Brachiaria decumbens, Diet B, consisting of 70% Brachiaria decumbens and 30% concentrate, and Diet C, consisting of 70% Brachiaria decumbens, 26% concentrate, and 4% rumen bypass fat for a period of 730 days. Feed intake was measured daily, while blood samples were collected for every eight months. Furthermore, body scores were noted prior to and at the end of the experimental period. The results showed that the average daily gain for buffaloes fed with Diet C was the highest. The DMI, BCS, FI, and FCR for the three groups showed significant (p < 0.05) differences, in the following order: Diet C > Diet B > Diet A. At the end of the two-year feeding trial, buffaloes fed with Diet B had significantly (p < 0.05) higher cholesterol levels than Diet A and Diet C. In addition, buffaloes fed with Diet C had significantly (p < 0.05) higher levels of serum total protein, growth hormone, and insulin-like growth factor-I hormone compared to Diet A and Diet B. On the other hand, buffaloes fed with Diet B and Diet C showed significant (p < 0.05) decrease in glucose levels. Supplemented diet improved the buffalos’ weight gain to achieve the market weight in a shorter period of time, thus, giving farmers a greater return. In conclusion, concentrate and bypass fat supplementations in the diet of water buffaloes improved the growth performance without adverse effect on the blood metabolites, which enabled better farmer profitability.


2019 ◽  
Vol 3 (2) ◽  
pp. 731-736 ◽  
Author(s):  
Henrique S Cemin ◽  
Jason C Woodworth ◽  
Mike D Tokach ◽  
Steve S Dritz ◽  
Joel M DeRouchey ◽  
...  

Abstract A total of 2,430 pigs (PIC 337 × 1050; Hendersonville, TN; initially 30.1 kg) were used in a 113-d growth trial to determine the effects of increasing dietary Zn on growth performance and carcass characteristics of finishing pigs raised under commercial conditions. Pens of pigs were assigned to be fed one of five dietary treatments in a randomized complete block design. Treatments consisted of 50, 87.5, 125, 162.5, or 200 mg/kg added Zn from Zn hydroxychloride (IntelliBond Z, Micronutrients, Indianapolis, IN). Two identical barns were used for a total of 18 pens per treatment with 27 pigs per pen. Experimental diets were fed in five phases and contained a vitamin-trace mineral premix without added Zn. Pens of pigs were weighed approximately every 2 wk to calculate average daily gain (ADG), average daily feed intake (ADFI), and gain-to-feed ratio (G:F). At the end of the experimental period, pigs were tattooed with a pen identification number and transported to a packing plant to measure hot carcass weight (HCW), backfat, loin depth, and calculated lean percentage. Data were analyzed block nested within barn as a random effect and pen as the experimental unit. From days 0 to 42, pigs fed diets with increasing added Zn had lower (linear, P = 0.043) ADFI and a tendency (P = 0.092) for lower ADG. From days 42 to 113, increasing added Zn resulted in a quadratic response (P = 0.042) for ADFI and a tendency (linear, P = 0.056) for improved G:F. Overall (days 0 to 113), there were tendencies for quadratic responses for ADFI (P = 0.073) and G:F (P = 0.059), with the greatest G:F observed when 125 mg/kg of Zn was fed. Increasing added Zn resulted in a linear increase (P &lt; 0.001) in daily Zn intake. There were no differences (P &gt; 0.10) in overall ADG, final body weight, HCW, backfat, loin depth, lean percentage, mortality, and removal rate. In conclusion, there were no improvements in ADG when feeding beyond 50 mg/kg added Zn; however, providing 125 mg/kg added Zn resulted in the greatest G:F.


2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 85-85
Author(s):  
Lucas Rodrigues ◽  
Michael O Wellington ◽  
J Caroline González-Vega ◽  
John K Htoo ◽  
Andrew G Van Kessel ◽  
...  

Abstract Functional amino acid supplementation during disease challenge enhances growth performance and immune status. The present study investigated the effect of duration of adaptation period to a functional amino acid (FAA)-supplemented diet on growth performance and immune status during a subsequent Salmonella challenge in pigs. Thirty-two mixed-sex weanling pigs (8 pigs/treatment; 11.6 ± 0.34 kg initial body weight) received either a basal diet without FAA supplementation throughout the experimental period (FAA-) or a diet containing a supplemented FAA profile (Thr, Met, and Trp at 120% of requirements) fed for either 0 (FAA+0), 1 (FAA+1) or 2 (FAA+2) wk pre- and 1 wk post-inoculation with Salmonella Typhimurium (ST). Pigs were orally inoculated with saline containing ST after the 2 wk pre-inoculation period and monitored for 1 wk post-inoculation. Pigs had ad libitum access to diets throughout the experiment. Performance parameters [average daily gain (ADG), feed intake, and gain:feed (GF)] were measured in the pre- and post-inoculation periods. Blood samples were collected on d 0, 4, and 7 post-inoculation for serum haptoglobin and albumin analysis. There was no effect of diet on pre-inoculation performance (P &gt; 0.05). Post-inoculation, FAA+2 pigs had the highest ADG (0.46 kg/d) and GF (0.63 kg/kg), FAA- the lowest (0.21 kg/d; 0.29 kg/kg), with FAA+0 (0.33 kg/d; 0.46 kg/kg) and FAA+1 (0.40 kg/d; 0.53 kg/kg) being intermediate (ADG, P &lt; 0.05, SEM=0.059; GF, P &lt; 0.05, SEM=0.099). Overall, albumin was higher in FAA+2 (35.25 g/L) and FAA+1 (34.63 g/L) pigs compared to FAA+0 (30.38 g/L) and FAA- (29.67 g/L) pigs (P &lt; 0.05, SEM=0.717). Furthermore, FAA+2 pigs had the lowest overall haptoglobin (0.90 g/L), FAA- the highest (1.54 g/L), with FAA+0 (1.32 g/L) and FAA+1 (1.06 g/L) being intermediate (P &lt; 0.05, SEM=0.111). In conclusion, a longer adaptation period to FAA supplementation improved performance and attenuated the immune response of pigs when exposed to an enteric disease challenge.


2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 417-419
Author(s):  
Koo Deokho ◽  
Serge Muhizi ◽  
Seok wooJung ◽  
Gao Shanshui ◽  
Song Junho ◽  
...  

Abstract Yeast products are extensively used as additives in livestock feed to improve their growth performance and intestinal health. A total of 90 crossbred finishing pigs were allocated to one of three treatments according to their BW and sex (2 barrows and 2 gilts) with 6 replicates per treatment and 5 pigs per pen for eight weeks. The dietary treatments were CON -basal diet, CON + 0.05% yeast hydrolysate (YH), CON + 0.1% YH. The yeast hydrolysate supplement used in this study was commercially prepared in the name of CALMORIN. The active ingredients of YH were 40% of crude protein, 3.5% of nucleotides, 23% of β-glucans,4.9% glutamic acid, and 15% of mannan-oligosaccharides. Data were subjected to the statistical analysis as a complete randomized design using the GLM procedures of SAS and the pen was used as the experimental unit. Linear and quadratic polynomial contrasts were performed to determine the effects yeast in the diet with P &lt; 0.05 indicating significance. Dietary inclusion of YH supplement linearly increased (P &lt; 0.05) body weight and average daily gain of pigs at wk 4,8, and overall trail period (respectively). Moreover, apparent total digestibility of dry matter, nitrogen, and energy showed linear (P &lt; 0.05) improvement in pigs fed graded level of YH supplementation. Also, the inclusion of YH supplementation linearly increased (P &lt; 0.05) the fecal microbial lactobacillus population. Furthermore, during the end of the trial, pigs fed YH tended to linearly increase (P &lt; 0.05) backfat thickness and lean meat percentage and linearly reduce drip loss (P &gt; 0.05) from meat sample on day 3 and 5 of storage. In conclusion the growth performance, nutrient digestibility, lactobacillus count, and meat quality of finishing pigs were positively enhanced by yeast hydrolysate supplement in the diet.


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