322 Impact of Feed Restriction and Fasting Suffered in Assembly Centers and Transportation on Energy Balance and Gut Permeability in Unweaned Angus-holstein Calves

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 176-176
Author(s):  
Lucía Pisoni ◽  
Sonia Marti ◽  
Marta Blanch ◽  
Jose J Pastor ◽  
Maria Devant
Keyword(s):  
Energy Balance ◽  
Serum Concentration ◽  
Mixed Models ◽  
Repeated Measures ◽  
Serum Glucose ◽  
Feed Restriction ◽  
Gut Permeability ◽  
Feed Withdrawal ◽  
Holstein Calves ◽  
Ad Libitum

Abstract Twenty unweaned Angus-Holstein calves (44.1 ± 2.0 kg; 14.7 ± 0.63 d) were used to evaluate the effects of feed restriction and fasting on energy balance (serum concentration of glucose, BHBA, and NEFA), and intestinal permeability (serum concentration of citrulline, Cr-EDTA, lactulose, and D-mannitol). Calves were randomly assigned to 1 of 4 treatments that simulated assembly center management and transportation; Control (CT; n = 5): from d-4 to -1, 2.5 L of milk replacer (MR) twice daily, concentrate and straw ad-libitum; Mild (MD; n = 5): only MR (d-4 to -1) as described for CT, and on d-1 feed withdrawal for 9 h; Moderate (MO; n = 5): only MR as described for CT and on d-1 feed withdrawal for 19 h; and Severe (SV; n = 5): only 2.5 L of a hydrate solution (HS) twice daily (d -4 to -1) on d-1 feed withdrawal for 19 h. From d 0 to day 7 all calves were fed MR, concentrate, and straw ad-libitum. Data were analyzed using mixed models with repeated measures. At d -1 for SV and d 0 in all restricted calves (MD, MO, SV), serum glucose concentration was lesser (P < 0.01) compared with CT. At d 0 NEFA and BHBA serum concentrations increased (P < 0.01) in SV calves compared with the other treatments. Serum citrulline concentration (P < 0.05) was lower for SV on d -1 and greater for CT on d 2 compared with other treatments. CT calves had the lesser concentration of Cr-EDTA (d -1 and d 0, P =0.04), lactulose (d 0, P =0.02), and D-mannitol (d 0, P =0.01) compared with MD, MO, and SV. The degree of dietary restriction, the type of liquid diet, and amount of fasting hours affected calves’ serum concentration of markers indicative of energy balance and gut permeability.

scholarly journals PSXII-5 Simulation of feed restriction and anorexia during auction market and transportation and its effects on concentrate intake and body weight recovery of unweaned crossbred Angus-Holstein calves

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 437-438
Author(s):  
Lucía Pisoni ◽  
Maria Devant ◽  
Sonia Marti
Keyword(s):  
Body Weight ◽  
Mixed Models ◽  
Repeated Measures ◽  
Feed Restriction ◽  
Auction Market ◽  
Feed Withdrawal ◽  
Liquid Feeding ◽  
Bull Calves ◽  
Holstein Calves ◽  
Ad Libitum

Abstract Twenty unweaned Angus-Holstein bull calves (44.1 ± 2.0 kg and 14.7 ± 0.63 d) were used to evaluate the effects of feed restriction and anorexia (simulating auction market and transport) on concentrate intake (CI) and body weight (BW) recovery. Calves were randomly assigned to 1 of 4 treatments; Control (CT; n = 5): from d-4 to -1, 2.5 L of milk replacer (MR) twice daily, concentrate and straw ad libitum; Mild (MD; n = 5): only MR (d-4 to -1) as described for CT, and at the end of d-1 feed withdrawal for 9 h; Moderate (MO; n = 5): only MR as described for CT and at the end of d-1 feed withdrawal for 19 h; and Severe (SV; n = 5): only 2.5 L of a hydrate solution (HS) twice daily (d -4 to -1) and at the end of d-1 feed withdrawal for 19 h. Simulating the arrival to the farm, from d 0 all calves were fed MR, concentrate and straw ad libitum. The CI was recorded daily, and BW was measured on d-4, -1 (restriction day), 0, 2 and 7. Data were analyzed using mixed models with repeated measures. BW was greater (P < 0.001) for CT compared with the rest from d0 to 7, while BW of SV was lesser compared with the rest from d-1 to d7. At d1 CI did not differ among treatments; however, at d 2 CI of MD, MO and SV was lesser (P < 0.05) compared with CT. At d5, CI of SV was similar to that for CT, and it was greater (P < 0.05) than CI of MD and MO. Calves BW and CI recovery was affected by the degree of restriction (9 vs 19 h) and by the type of liquid feeding (MR or HS) offered in long restriction periods.

scholarly journals 1 Physiological and performance recovery after fourteen hours of feed restriction or transportation in Holstein bulls fed high concentrate diets

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 2-3
Author(s):  
Sonia Marti ◽  
Elena Garcia ◽  
Christine Gerard ◽  
Joan Grau ◽  
Nicolas Cirier ◽  
...  
Keyword(s):  
Repeated Measures ◽  
Feed Restriction ◽  
Amyloid A ◽  
Performance Recovery ◽  
Treatment Data ◽  
Access To Water ◽  
Holstein Calves ◽  
Holstein Bulls ◽  
And Performance ◽  
Beta Hydroxybutyrate

Abstract One hundred and eight Holstein calves (225 ± 1.1 kg and 187 ± 5.2 d) were used to evaluate the physiological and performance recovery after 14 h transportation or feed restriction. Calves were distributed into 6 pens (2 pens/treatment) according to control (CTR, n = 36) calves with ad libitum access to concentrate, straw and water; restricted (RES, n = 36) calves with concentrate restriction but with access to water and straw for 14 h; and transported (TRA, n = 36) calves that were loaded into a trailer and transported without feed or water for 14 h. On days 0, 7, 21, and 35 BW was recorded. Concentrate intake were recorded daily. Blood samples for non-esterified fatty acids (NEFA), beta-hydroxybutyrate (BHBA), and serum amyloid-A (SAA) were collected at -24, -14, 0 h, and 6, 24, and 168 h post-treatment. Data were analyzed using mixed models with repeated measures. At 24 h, RES and TRA had greater (P < 0.05) concentrate intake compared with CTR. However, from d 7 to 35 after treatments, only TRA had similar concentrate intake than CTR, while RES had lesser (P < 0.05) concentrate intake than CTR and TRA. RES at 6 h had greater (P < 0.05) NEFA concentrations than TRA, and NEFA concentrations were still higher for the RES and TRA groups than those for the CTR after 24 h. After 24 h concentrations of serum BHBA for TRA and RES were significantly greater (P < 0.05) when compared with those for the CTR. Serum concentration of SAA for TRA and RES was greater (P < 0.05) than CTR until 168 h. Results showed similar effects of 14 h of feed restriction and transportation of calves on serum anorexia and inflammation parameters; however, feed intake was recovered after d 35 in transported calves but not in feed restricted calves without transportation.

scholarly journals Feed Restriction Reveals Distinct Serum Metabolome Profiles in Chickens Divergent in Feed Efficiency Traits

Metabolites ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 38 ◽  
Author(s):  
Barbara Metzler-Zebeli ◽  
Sina-Catherine Siegerstetter ◽  
Elizabeth Magowan ◽  
Peadar Lawlor ◽  
Niamh O’Connell ◽  
...  
Keyword(s):  
Feed Intake ◽  
Feed Efficiency ◽  
Energy Demand ◽  
Body Weight Gain ◽  
Serum Glucose ◽  
Arachidonic Acid Metabolism ◽  
Feed Restriction ◽  
Physiological Factors ◽  
Ad Libitum ◽  
Almost All

Restrictive feeding influences systemic metabolism of nutrients; however, this impact has not been evaluated in chickens of diverging feed efficiency. This study investigated the effect of ad libitum versus restrictive feeding (85% of ad libitum) on the serum metabolome and white blood cell composition in chickens of diverging residual feed intake (RFI; metric for feed efficiency). Blood samples were collected between days 33 and 37 post-hatch. While serum glucose was similar, serum uric acid and cholesterol were indicative of the nutritional status and chicken’s RFI, respectively. Feed restriction and RFI rank caused distinct serum metabolome profiles, whereby restrictive feeding also increased the blood lymphocyte proportion. Most importantly, 10 amino acids were associated with RFI rank in birds, whereas restrictive feeding affected almost all detected lysophosphatidylcholines, with 3 being higher and 6 being lower in restrictively compared to ad libitum fed chickens. As indicated by relevance networking, isoleucine, lysine, valine, histidine, and ornithine were the most discriminant for high RFI, whereas 3 biogenic amines (carnosine, putrescine, and spermidine) and 3 diacyl-glycerophospholipids (38:4, 38:5, and 40:5) positively correlated with feed intake and body weight gain, respectively. Only for taurine, feed intake mostly explained the RFI-associated variation, whereas for most metabolites, other host physiological factors played a greater role for the RFI-associated differences, and was potentially related to insulin-signaling, phospholipase A2, and arachidonic acid metabolism. Alterations in the hepatic synthesis of long-chain fatty acids and the need for precursors for gluconeogenesis due to varying energy demand may explain the marked differences in serum metabolite profiles in ad libitum and restrictively fed birds.

scholarly journals Calorie Restriction Modulates Reproductive Development and Energy Balance in Pre-Pubertal Male Rats

Nutrients ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 1993 ◽  
Author(s):  
Guilherme Rizzoto ◽  
Deepa Sekhar ◽  
Jacob C. Thundathil ◽  
Prasanth K. Chelikani ◽  
John P. Kastelic
Keyword(s):  
Body Composition ◽  
Energy Balance ◽  
Reproductive Development ◽  
Negative Energy ◽  
Male Rats ◽  
Plasma Testosterone ◽  
Feed Restriction ◽  
Testis Weight ◽  
Sprague Dawley ◽  
Ad Libitum

The objective was to determine effects of feed restriction and refeeding on reproductive development and energy balance in pre-pubertal male rats. Sprague Dawley rats (n = 32, 24 days old, ~65 g), were randomly allocated into four treatments (n = 8/treatment): (1) Control (CON, ad libitum feed; (2) Mild Restriction (MR, rats fed 75% of CON consumption); (3) Profound Restriction (PR, 50% of CON consumption); or (4) Refeeding (RF, 50% restriction for 14 days, and then ad libitum for 7 days). Feed restriction delayed reproductive development and decreased energy balance and tissue accretion, with degree of reproductive and metabolic dysfunctions related to restriction severity. In RF rats, refeeding largely restored testis weight, sperm production (per gram and total), plasma IGF-1, leptin and insulin concentrations and energy expenditure, although body composition did not completely recover. On Day 50, more CON and RF rats than PR rats were pubertal (5/6, 4/5 and 1/6, respectively; plasma testosterone >1 ng/mL) with the MR group (4/6) not different. Our hypothesis was supported: nutrient restriction of pre-pubertal rats delayed reproductive development, induced negative energy balance and decreased metabolic hormone concentrations (commensurate with restriction), whereas short-term refeeding after profound restriction largely restored reproductive end points and plasma hormone concentrations, but not body composition.

scholarly journals PSIX-6 Gastrointestinal microbiome of calves fed solid diets containing different levels and sources of NDF

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 418-419
Author(s):  
Gercino F Virgínio Júnior ◽  
Milaine Poczynek ◽  
Ana Paula Silva ◽  
Ariany Toledo ◽  
Amanda Cezar ◽  
...  
Keyword(s):  
Illumina Miseq ◽  
Diversity Indices ◽  
Dairy Calves ◽  
Rrna Gene ◽  
Gastrointestinal Microbiome ◽  
Fecal Microbiome ◽  
Miseq Platform ◽  
Holstein Calves ◽  
Ad Libitum ◽  
Different Levels

Abstract Different levels and sources of NDF can modify the gastrointestinal microbiome. This study evaluated 18 Holstein calves housed in not-bedded suspended individual cages and fed one of three treatments: 22NDF - conventional starter containing 22% NDF (n = 7); 31NDF - starter with 31% NDF, replacing part of the corn by soybean hull (n = 6); and 22NDF+H - conventional starter with 22% NDF plus coast-cross hay ad libitum (n = 5). All animals received 4 L of milk replacer daily (24% CP; 18.5% fat; diluted to 12.5% solids), divided into two meals, being weaned at 8th week of age. After weaning, animals were housed in tropical shelters, fed with the respective solid diet and coast-cross hay ad libitum for all treatments. To evaluate the microbiome, ruminal fluid samples were collected using a modified Geishauser oral probe at weeks 2, 4, 6, 8 and 10, two hours after the morning feeding, and fecal samples were collected at birth (0) and at weeks 1, 2, 4, 8 and 10. The microbial community was determined by sequencing V3 and V4 region amplicons of the 16S rRNA gene that was amplified by PCR and sequenced by the Illumina MiSeq platform. Ruminal microbiome had no differences in diversity for the effects of weeks, treatments or interaction of both factors (Table 1). In feces, the diversity indices and evenness were higher for 22NDF+H when compared to 22NDF, with no difference for 31NDF. All indices were significantly affected by calves age. At birth, calves had the greatest diversity and richness. Week 1 and 2 had less evenness and diversity. Bacteroidota, Firmicutes_A and Firmicutes_C were the most abundant phylum in rumen and feces. The supply of hay was only effective in modifying the fecal microbiome of dairy calves, suggesting a resilience in the ruminal microbiome.

scholarly journals Clinical characterization of dysautonomia in long COVID-19 patients

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Nicolas Barizien ◽  
Morgan Le Guen ◽  
Stéphanie Russel ◽  
Pauline Touche ◽  
Florent Huang ◽  
...  
Keyword(s):  
Mixed Models ◽  
Repeated Measures ◽  
Physical Medicine ◽  
Persistent Symptoms ◽  
Index Changes ◽  
Changes Over Time ◽  
And Control ◽  
Testing Group ◽  
Over Time

AbstractIncreasing numbers of COVID-19 patients, continue to experience symptoms months after recovering from mild cases of COVID-19. Amongst these symptoms, several are related to neurological manifestations, including fatigue, anosmia, hypogeusia, headaches and hypoxia. However, the involvement of the autonomic nervous system, expressed by a dysautonomia, which can aggregate all these neurological symptoms has not been prominently reported. Here, we hypothesize that dysautonomia, could occur in secondary COVID-19 infection, also referred to as “long COVID” infection. 39 participants were included from December 2020 to January 2021 for assessment by the Department of physical medicine to enhance their physical capabilities: 12 participants with COVID-19 diagnosis and fatigue, 15 participants with COVID-19 diagnosis without fatigue and 12 control participants without COVID-19 diagnosis and without fatigue. Heart rate variability (HRV) during a change in position is commonly measured to diagnose autonomic dysregulation. In this cohort, to reflect HRV, parasympathetic/sympathetic balance was estimated using the NOL index, a multiparameter artificial intelligence-driven index calculated from extracted physiological signals by the PMD-200 pain monitoring system. Repeated-measures mixed-models testing group effect were performed to analyze NOL index changes over time between groups. A significant NOL index dissociation over time between long COVID-19 participants with fatigue and control participants was observed (p = 0.046). A trend towards significant NOL index dissociation over time was observed between long COVID-19 participants without fatigue and control participants (p = 0.109). No difference over time was observed between the two groups of long COVID-19 participants (p = 0.904). Long COVID-19 participants with fatigue may exhibit a dysautonomia characterized by dysregulation of the HRV, that is reflected by the NOL index measurements, compared to control participants. Dysautonomia may explain the persistent symptoms observed in long COVID-19 patients, such as fatigue and hypoxia. Trial registration: The study was approved by the Foch IRB: IRB00012437 (Approval Number: 20-12-02) on December 16, 2020.

Changes in multiple sclerosis symptoms are associated with changes in work productivity of people living with multiple sclerosis

2021 ◽  
pp. 135245852199455
Author(s):  
Barnabas Bessing ◽  
Mohammad A Hussain ◽  
Suzi B Claflin ◽  
Jing Chen ◽  
Leigh Blizzard ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Mixed Models ◽  
Repeated Measures ◽  
Work Productivity ◽  
Annual Change ◽  
Individual Variations ◽  
Unit Increase ◽  
The Mean ◽  
Employment Rates ◽  
Study Participants

Background: While employment rates have increased in people with multiple sclerosis (PwMS), little is known about the longitudinal trends of work productivity. Objective: To describe the longitudinal patterns of work productivity and examine the factors associated with annual change of work productivity of PwMS. Methods: Study participants were employed participants of the Australian MS Longitudinal Study (AMSLS) followed from 2015 to 2019 with at least two repeated measures ( n = 2121). We used linear mixed models to examine if the within-individual variations in MS symptoms are associated with changes in work productivity. Results: The mean annual change in work productivity between 2015 and 2019 was −0.23% ( SD = 18.68%). Not the actual severity of symptoms but rather the changes in severity of symptoms that are associated with change in work productivity in the same year. In a multivariable model, every unit increase in mean annual change in ‘pain and sensory symptoms’, ‘feelings of anxiety and depression’, and ‘fatigue and cognitive symptoms’ were independently associated with 2.43%, 1.55% and 1.01% annual reductions in work productivity, respectively. Conclusion: Individual changes in work productivity are largely driven by the changes in symptom severity rather than the absolute severity. Stabilising/improving MS symptoms might improve work productivity.

scholarly journals 214 The effects of an 18-hour transit or complete feed and water restriction on feed bunk displacements and need preferences in growing feedlot steers

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 5-5
Author(s):  
Katie J Heiderscheit ◽  
Erin Deters ◽  
Alyssa Freestone ◽  
Joshua Peschel ◽  
Stephanie L Hansen
Keyword(s):  
Repeated Measures ◽  
Dry Matter ◽  
Treatment Time ◽  
Dry Matter Intake ◽  
Feed Restriction ◽  
Water Restriction ◽  
Trained Observer ◽  
Aggressive Interactions ◽  
Feedlot Steers ◽  
Effect Of Treatment

Abstract The objective was to investigate effects of 18 h feed and water restriction or transit on cattle behavior. Angus-cross steers (36; 353 ± 33 kg) were housed in pens of 6 and assigned to treatments: control (CON), full access to feed and water; deprived (DEPR), no feed or water for 18 h; or transported (TRANS), trucked for 18 h. Individual BW (n = 12 steers/treatment) was recorded on d 0, 1, 3, 8, and 14, and individual dry matter intake (DMI) was determined via GrowSafe bunks. Bunk displacements on d 1 were recorded for each pen (n = 2 pens/treatment) by one trained observer continuously for 2 h in 10 min intervals via video analysis. Steer need preferences were assessed as time individuals took to perform behaviors (eat, drink, lay) after treatments ended on d 1. Data were analyzed using Proc Mixed of SAS with fixed effect of treatment; displacements, BW, and DMI were analyzed as repeated measures. Upon return to pens, time to eat or drink did not differ between DEPR and TRANS (P ≥ 0.17), but time to lay was 70.5 min for DEPR vs. 16.5 min for TRANS (P = 0.01). Displacements were greater for DEPR than CON or TRANS during the first 90 min after accessing feed, while CON displaced more frequently than TRANS for the first 30 min (treatment × time; P = 0.02). While DMI for TRANS was not recovered until d 2, DEPR and CON had similar DMI on d 1 (treatment × day; P < 0.01). Similarly, TRANS BW were, and DEPR tended to be, lesser than CON on d 1; however, BW among treatments were not different on other days (treatment × day; P < 0.01). Thus, restricting feed increases aggressive interactions at the bunk and cattle trucked long distances are quick to lay down when allowed. These behaviors should be considered when managing an unintentional feed restriction event or receiving cattle into the feedlot.

scholarly journals Acute effects of mustard, horseradish, black pepper and ginger on energy expenditure, appetite,ad libitumenergy intake and energy balance in human subjects

2012 ◽  
Vol 109 (3) ◽  
pp. 556-563 ◽  
Author(s):  
N. T. Gregersen ◽  
A. Belza ◽  
M. G. Jensen ◽  
C. Ritz ◽  
C. Bitz ◽  
...  
Keyword(s):  
Energy Balance ◽  
Treatment Effects ◽  
Human Subjects ◽  
Statistical Significance ◽  
Black Pepper ◽  
Normal Weight ◽  
Acute Effects ◽  
Significant Treatment ◽  
Ad Libitum ◽  
Few Data

Chilli peppers have been shown to enhance diet-induced thermogenesis (DIT) and reduce energy intake (EI) in some studies, but there are few data on other pungent spices. The primary aim of the present study was to test the acute effects of black pepper (pepper), ginger, horseradish and mustard in a meal on 4 h postprandial DIT. The secondary aim was to examine the effects on subjective appetite measures,ad libitumEI and energy balance. In a five-way placebo-controlled, single-blind, cross-over trial, twenty-two young (age 24·9 (sd4·6) years), normal-weight (BMI 21·8 (sd2·1) kg/m2) males were randomly assigned to receive a brunch meal with either pepper (1·3 g), ginger (20 g), horseradish (8·3 g), mustard (21 g) or no spices (placebo). The amounts of spices were chosen from pre-testing to make the meal spicy but palatable. No significant treatment effects were observed on DIT, but mustard produced DIT, which tended to be larger than that of placebo (14 %, 59 (se3)v.52 (se2) kJ/h, respectively,P= 0·08). No other spice induced thermogenic effects approaching statistical significance. Subjective measures of appetite (P>0·85),ad libitumEI (P= 0·63) and energy balance (P= 0·67) also did not differ between the treatments. Finally, horseradish decreased heart rate (P= 0·048) and increased diastolic blood pressure (P= 0·049) compared with placebo. In conclusion, no reliable treatment effects on appetite, EI or energy balance were observed, although mustard tended to be thermogenic at this dose. Further studies should explore the possible strength and mechanisms of the potential thermogenic effect of mustard actives, and potential enhancement by, for example, combinations with other food components.

scholarly journals Associations between Longitudinal Patterns of Substance Use and Anxiety and Depression Symptoms among a Sample of Canadian Secondary School Students

2021 ◽  
Vol 18 (19) ◽  
pp. 10468
Author(s):  
Gillian C. Williams ◽  
Karen A. Patte ◽  
Mark A. Ferro ◽  
Scott T. Leatherdale
Keyword(s):  
Substance Use ◽  
Mixed Models ◽  
Repeated Measures ◽  
Latent Class ◽  
Treatment Strategies ◽  
Anxiety And Depression ◽  
Depression Symptoms ◽  
Cigarette Use ◽  
School Students ◽  
Relevant Covariates

The objective of this study is to examine the longitudinal associations between latent classes of substance use and anxiety and depression scores among youth who use substances. This study uses data from three waves (Wave 1: 2017/18, Wave 2: 2018/19, and Wave 3: 2019/20) of the COMPASS study. Students in grades 9 and 10 who reported substance use at baseline (n = 738) report their substance use (alcohol, cannabis, cigarettes, and e-cigarettes) and anxiety and depression symptoms at each wave. A Repeated Measures Latent Class Analysis (RMLCA) is used to determine substance use classes, and mixed models are used to examine the associations between substance use classes and anxiety and depression. We identify three classes of substance use: (1) occasional alcohol and e-cigarette use, (2) escalating poly-substance use, and (3) consistent poly-substance use. After controlling for relevant covariates, consistent poly-substance use is associated with depression (Female OR: 1.24 [95%CI: 0.46, 2.02]; Male OR 1.13 [95%CI: 0.38, 1.87]) but not anxiety. Escalating poly-substance use is associated with depression among males (OR 0.72 [95%CI: 0.10, 1.33]). These findings should be taken into consideration when creating prevention programming and treatment strategies for adolescents. Substance use programming should be comprehensive, consider multiple substances, and be cognizant of symptoms of mental illness, particularly depression.

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