The impact of dietary supplementation of arginine during gestation in a commercial swine herd: II. Offspring performance

Abstract Arginine (Arg) is an important amino acid of pig fetal development; however, whether Arg improves postnatal performance is ill-defined. Therefore, the influence of Arg supplementation at different gestational stages on offspring performance was evaluated in a commercial swine herd. Sows (n = 548) were allocated into 4, diet by stage of gestation treatments: Control (n = 143; 0% suppl. Arg), or dietary treatments supplemented with 1% L-Arg (free-base; Ajinomoto Animal Nutrition North America, Inc., Chicago, IL): from 15 to 45 d of gestation (n = 138; Early-Arg); 15 d of gestation to farrowing (n = 139; Full-Arg); and from day 85 of gestation to farrowing (n = 128; Late-Arg). All offspring were individually identified and weighed at birth; at weaning, a subset was selected for evaluation of carcass performance at market. All data were analyzed using birth weight (BiWt) and age as covariates. Wean weights (WW) and prewean (PW) ADG tended to increase (P = 0.06) in progeny from sows supplemented with Arg, as compared to progeny from Control sows. Preplanned contrast comparisons revealed an increased (P = 0.03) BiWt for pigs from sows receiving 1% L-Arg prior to day 45 of gestation (Early-Arg and Full-Arg; 1.38 kg/pig), as compared to pigs from sows not supplemented prior to day 45 of gestation (Control and Late-Arg; 1.34 kg/pig). No difference in BiWt was observed (1.36 kg/pig; P = 0.68) for Arg supplementation after day 85 of gestation (Full-Arg and Late-Arg), as compared to those not receiving Arg supplementation after day 85 (Control and Early-Arg); although WW and PW ADG were greater (P = 0.02), respectively. A 3.6% decrease (P = 0.05) in peak lean accretion ADG occurred when dams received 1% L-Arg prior to day 45 of gestation (Early-Arg and Full-Arg), however, no other significant differences were detected in finishing growth parameters or carcass characteristics (P ≥ 0.1). Pig mortality rates tended (P = 0.07) to decrease in progeny of dams supplemented Arg after day 85 (3.6%) compared to dams not provided additional Arg during late gestation (4.9%). Collectively, these data suggest that Arg provided during late gestation may improve WW and PW ADG, however, finishing performance was not affected. While Arg supplementation provided some moderate production benefits, further investigation is warranted to comprehensively understand the gestational timing and biological role of Arg supplementation during fetal and postnatal development in commercial production systems.

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PSVI-10 Impact of Soybean Inclusion Level in Late Gestation on Offspring Performance

Journal of Animal Science ◽

10.1093/jas/skab054.363 ◽

2021 ◽

Vol 99 (Supplement_1) ◽

pp. 222-222

Author(s):

Abigail R Loucks ◽

Dana M van Sambeek ◽

Elizabeth A Hines

Keyword(s):

Growth Performance ◽

Cost Effective ◽

Late Gestation ◽

Immunoglobulin Production ◽

Offspring Performance ◽

Body Weights ◽

Inclusion Rate ◽

Inclusion Level ◽

Dietary Treatments ◽

The Impact

Abstract Inclusion of soybean meal (SBM) in gestation diets has been decreasing as producers replace amino acid sources with more cost-effective ingredients. In particular, SBM components have been associated with immunoglobulin production and increased nutrition in late gestation has been associated with limited growth performance increases. Yet, it is unknown if SBM inclusion levels in late gestation diets influences offspring growth during the lactation and nursery phases. Therefore, the objective of this project is to investigate the impact of SBM inclusion rates in late gestation on offspring performance. Sows (n = 63) were assigned to one of four diets, 14 days prior to farrowing: A (n = 13; 0% SBM, 13.5% crude protein (CP), B (n = 16; 10% SBM, 13.5% CP), C (n = 17; 30% SBM, 21.5% CP), D (n = 17; 0% SBM, 21.5% CP). Growth performance of piglets was evaluated during lactation. Piglets per diet: A (n = 170), B (n = 183), C (n = 200), D (n = 228). Body weights were recorded daily from birth through d10 of lactation then at d15 and every 7 days until weaning. Colostrum and milk at d15 were evaluated for immunoglobulin concentration. After weaning, weights were recorded at two-week intervals during the nursery phase. No significant effect of gestation diet was observed for birth weight (P = 0.27) or ADG from day 0 to weaning (P = 0.78). Wean weight (WW), post-wean weight 1 (PW1), and post-wean weight 2 (PW2) were not different across dietary treatments (P > 0.28). Immunoglobulin production was similar across diets. These results suggest that the inclusion rate of SBM content in the last 14 days of gestation has no impact on offspring performance. Continued research is needed into understanding the impact of nutritional status of the sow during gestation on long-term offspring performance.

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Mechanism of persistence of indigenous bifidobacteria under the impact of acetate in the human colon biotope

Journal of microbiology epidemiology immunobiology ◽

10.36233/0372-9311-86 ◽

2021 ◽

Vol 98 (3) ◽

pp. 276-282

Author(s):

O. V. Bukharin ◽

S. V. Andryuschenko ◽

N. B. Perunova ◽

E. V. Ivanova

Keyword(s):

Acetic Acid ◽

Ammonium Acetate ◽

Growth Parameters ◽

Bifidobacterium Longum ◽

Human Colon ◽

Bifidobacterium Bifidum ◽

Gram Positive ◽

Gram Positive Bacteria ◽

The Impact

Aim. To determine the role of the acetate in the persistence of indigenous bifidobacteria in the colon biotope through the lysozyme resistance in model conditions of the acetylation–deacetylation of peptidoglycan.Materials and methods. The study was performed on 16 strains of the two indigenous bifidobacteria speсies: Bifidobacterium bifidum и Bifidobacterium longum subsp. longum. Bifidobacteria was cultivated in the 0.6% O2 and 9% CO2 atmosphere at the temperature 37ºС in CO2 incubator for 48 hours. The production of the acetate by the bifidobacteria was determined by gas chromatography. The effect of the acetate on the lysozyme resistance of non-indigenous gram-positive bacteria was determined on the Listeria monocytogenes ICIS-280 model strain by the cultivation in LB-Lennox broth with ammonium acetate added in the concentration range matching the concentrations produced by the studied bifidobacteria, in lysozyme serial dilutions at final concentrations 5 μg/ml to 40 μg/ml within 24 hours.Results. It was found that the acetate release of Bifidobacterium longum subsp. longum was on average two times higher that of Bifidobacterium bifidum (27.0 and 14.7 mmol/liter, respectively) and was quite consistent with the concentrations of acetic acid determined in the intestinal contents (up to 50 mmol/liter). Cultivation of bifidobacteria in a medium with lysozyme, ammonium acetate and their combination did not have a significant impact on their growth parameters at the maximum used concentrations of these substances. In the test strain, the addition of ammonium acetate in the range created by bifidobacteria caused a decrease in the minimum inhibitory concentration of lysozyme by more than two times — from 40 μg/ml to less than 20 μg/ml. In the control medium without lysozyme, no inhibition of the growth of the indicator culture was observed up to the maximum concentrations of ammonium acetate.Conclusion. The mechanism of persistence (survival) of indigenous bifidobacteria in the human intestinal biotope has been identified, which is associated with the production of acetic acid at a level that selectively suppresses lysozyme resistance of non-indigenous gram-positive microbiota viareversible deacetylation of peptidoglycan. This allows indigenous bifidobacteria to maintain a stable dominant position in the biotope.

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Analysis of the Basal Plane Dislocation Density and Thermomechanical Stress during 100 mm PVT Growth of 4H-SiC

Materials ◽

10.3390/ma12132207 ◽

2019 ◽

Vol 12 (13) ◽

pp. 2207 ◽

Cited By ~ 2

Author(s):

Johannes Steiner ◽

Melissa Roder ◽

Binh Duong Nguyen ◽

Stefan Sandfeld ◽

Andreas Danilewsky ◽

...

Keyword(s):

Basal Plane ◽

Growth Parameters ◽

Electronic Devices ◽

Defect Distribution ◽

Thermomechanical Stress ◽

X Ray ◽

Basal Plane Dislocation ◽

The Impact ◽

Koh Etching

Basal plane dislocations (BPDs) in 4H silicon carbide (SiC) crystals grown using the physical vapor transport (PVT) method are diminishing the performance of SiC-based power electronic devices such as pn-junction diodes or MOSFETs. Therefore, understanding the generation and movement of BPDs is crucial to grow SiC suitable for device manufacturing. In this paper, the impact of the cooldown step in PVT-growth on the defect distribution is investigated utilizing two similar SiC seeds and identical growth parameters except for a cooldown duration of 40 h and 70 h, respectively. The two resulting crystals were cut into wafers, which were characterized by birefringence imaging and KOH etching. The initial defect distribution of the seed wafer was characterized by synchrotron white beam X-ray topography (SWXRT) mapping. It was found that the BPD density increases with a prolonged cooldown time. Furthermore, small angle grain boundaries based on threading edge dislocation (TED) arrays, which are normally only inherited by the seed, were also generated in the case of the crystal cooled down in 70 h. The role of temperature gradients inside the crystal during growth and post-growth concerning the generation of shear stress is discussed and supported by numerical calculations.

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32 Nutritional management of forage-based cow-calf operations

Journal of Animal Science ◽

10.1093/jas/skz258.071 ◽

2019 ◽

Vol 97 (Supplement_3) ◽

pp. 36-36

Author(s):

Philipe Moriel

Keyword(s):

Bos Indicus ◽

Warm Season ◽

Postnatal Growth ◽

Late Gestation ◽

Sugarcane Molasses ◽

Performance Study ◽

Offspring Performance ◽

Cattle Industry ◽

Dried Distillers Grains ◽

The Impact

Abstract The beef cattle industry in the Southeastern United States relies primarily on forages that not always meet the cowherd nutritional requirements, resulting in reduced performance if supplemental nutrients are not provided. Although precalving nutrition modulates postnatal offspring performance, most studies did not utilize Bos indicus-influenced cows consuming low-quality, warm-season forages. Hence, 3 studies evaluated the impact of different supplementation strategies during gestation on subsequent cow and calf performance.Study 1focused on timing of dried distillers grains (DDG) supplementation during late-gestation. Treatments initiated 84 d before calving and consisted of no supplementation, 1 kg/d of DDG from d 0 to 84, or 2 kg/d of DDG from d 0 to 42. Timing of prepartum DDG supplementation did not impact cow reproduction, but calf pre-weaning growth was enhanced only when cows received longer vs. shorter or no periods of prepartum supplementation. Study 2evaluated the use of prepartum supplementation of sugarcane molasses/urea added or not with DL-methionine hydroxyl analog. At 56 d before calving, Brangus heifers received no supplementation or molasses/urea (7.2 kg DM/wk) with or without methionine (105 g/wk; Alimet, Novus) until calving. Sugarcane molasses/urea supplementation improved cow BCS at calving and calf postnatal growth compared to no supplementation. However, supplemental methionine did not further enhance cow and calf growth performance. Study 3 compared winter vs. annual supplementation of sugarcane molasses/urea or range cubes. Starting on d 0 (pregnancy diagnosis; May), Brangus cows were assigned to receive molasses/urea supplementation during winter only (calving until end of breeding season) or year-round supplementation of molasses/urea or range cubes. Treatments had similar annual supplement DM amount (272 kg/yr). Year-round supplementation of molasses/urea and range cubes enhanced cow BCS at calving but did not impact pregnancy rate of cows and calf weaning weights than winter molasses/urea supplementation.

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PSII-2 The impact of essential oil supplementation on sow milk composition and fecal dry matter

Journal of Animal Science ◽

10.1093/jas/skz122.403 ◽

2019 ◽

Vol 97 (Supplement_2) ◽

pp. 229-229

Author(s):

Shannon L Dierking ◽

H J Monegue ◽

Merlin D Lindemann

Keyword(s):

Dry Matter ◽

Milk Composition ◽

Late Gestation ◽

Gross Energy ◽

Group 3 ◽

Group 2 ◽

The Individual ◽

Dietary Treatments ◽

The Impact ◽

Group 1

Abstract The transition from gestation to lactation greatly impacts the sow body. The expulsion of piglets, placenta, and fluids can result in dehydration and consequently constipation post-farrowing. The objective of this study was to determine the effects of an EO product (tradename Absorbezz) on sow fecal dry matter and milk composition. A total of 101 late-gestation sows from three farrowing groups (Group 1: 110 ± 3 d GEST, 263 ± 28 kg initial BW, Group 2: 109 ± 1 d GEST, 259 ± 26 kg initial BW, Group 3: 73 ± 5 d GEST, 238 ± 30 kg initial BW) were allotted to one of two treatments based on parity and BW in each group (Groups 1 and 2: CON: No EO, TRT: 10 mL/d of EO top-dressed; Group 3 received the EO as part of their diet, with a range dependent on the individual sow’s daily feed intake). Sows remained on their respective dietary treatments throughout the remainder of gestation and lactation. Samples were collected from each group (feces; d 110 of gestation, d 4 to 6 and day 14 to 17 of lactation; milk (Group 2 and 3); d 14 to 17). Analysis of variance was performed using PROC GLM of SAS. The TRT group did not experience any differences in the components of fat, protein, and total solids (P > 0.10), there was an increase in lactose (5.97 vs 5.84, P = 0.04), but a decrease in solids non-fat (10.91 vs. 11.00, P = 0.07), and gross energy compared to those of the CON group (4.29 mJ/kg vs. 4.45 mJ/kg, P = 0.08). Fecal DM was not affected at any collection time (P > 0.10). Overall, the addition of EO did not affect fecal DM, but did influence several milk components.

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PSII-10 Effect of gilt feeding level and length of increased feeding level in late gestation on piglet birth weight

Journal of Animal Science ◽

10.1093/jas/skz122.410 ◽

2019 ◽

Vol 97 (Supplement_2) ◽

pp. 233-233

Author(s):

James K Quick ◽

Mark Knauer

Keyword(s):

Birth Weight ◽

Litter Size ◽

Body Condition ◽

Fixed Effects ◽

Body Condition Score ◽

Dietary Treatment ◽

Late Gestation ◽

Feeding Level ◽

Dietary Treatments ◽

The Impact

Abstract The objective of the study was to evaluate the impact of increasing gilt feeding level in late gestation, for different durations, on piglet quality. Gilts (n = 472) were allocated to one of five dietary treatments in a 2 × 2 factorial + control design at a commercial farm in eastern North Carolina. Hence gilts were fed 1.82 kg of feeding until farrowing (Control) or feeding level was increased by either 0.68 or 1.36 kg at either day 93 (d93) or 100 (d100) of gestation. Treatments were randomly assigned by pen (5 to 6 gilts per pen). The gestation diet contained 2,979 Kcal/kg ME and 0.58% SID lysine. Gilt body condition score was captured at day 93 of gestation using a sow body condition caliper (thin = < 12, ideal = 12 to 15, fat = > 15). Piglet birth weights were captured within 24 h of farrowing and piglets were ear notched by treatment prior to cross-fostering. Data were analyzed using PROC GLM with fixed effects of dietary treatment, contemporary group and covariates of litter size and sow functional teat number when applicable. Average gilt caliper score at d 93 of gestation was 17.0. Gilt caliper score did not differ (P > 0.05) across dietary treatments. Mean piglet birth weight did not differ (P > 0.05) between the five dietary treatments or the main effects of feeding level or length of feeding level. A one piglet increase in litter size reduced (P < 0.01) mean piglet birth weight by 30 g. An increase of one functional sow teat increased (P < 0.05) litter size at weaning of the biological sow by 0.28 piglets. Results suggest increasing gilt feeding level in late gestation does not impact mean piglet birth weight when gilts are over conditioned.

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MON-016 Neural Circuits and Hormonal Mechanisms Underlying the Negative Impact of Stress on Pregnancy Outcomes

Journal of the Endocrine Society ◽

10.1210/jendso/bvaa046.1609 ◽

2020 ◽

Vol 4 (Supplement_1) ◽

Author(s):

Neta Gotlieb ◽

Kathryn E Wilsterman ◽

Samantha L Finn ◽

Madison Browne ◽

Damhee Hu ◽

...

Keyword(s):

Pregnancy Outcomes ◽

Negative Impact ◽

Prolactin Secretion ◽

Late Gestation ◽

Female Reproductive Health ◽

Neuroendocrine Mechanisms ◽

Pregnant Mice ◽

The Impact ◽

The Brain

Abstract Despite numerous findings detailing the negative impact of stress on female reproductive health, the means by which stress acts on the CNS and periphery to compromise reproductive success remains poorly understood. As a result, the current study sought to clarify the neuroendocrine mechanisms by which stress acts on the brain to deleteriously influence pregnancy outcomes. Reproduction is regulated by the hypothalamo-pituitary-gonadal (HPG) axis, with hypothalamic gonadotropin-releasing hormone (GnRH) neurons representing the final, common pathway of this axis. Cells expressing the inhibitory neuropeptide, RFamide-related peptide-3 (RFRP-3), lie upstream of the GnRH system and are markedly regulated by environmental and psychosocial factors, including stress. In the present study, we asked whether RFRP-3 neurons mediate the effects of stress on pregnancy outcomes through the regulation of prolactin secretion, as prolactin is critical for pregnancy maintenance. More specifically, because specialized hypothalamic dopaminergic neurons, namely tubero-infundibular dopaminergic (TIDA) neurons, are major regulators of prolactin secretion, we hypothesized that RFRP-3 neurons directly target TIDA cells to negatively influence fetal development. To test this possibility, we subjected pregnant mice to chronic restraint stress for the first half of pregnancy and performed a broad screen of hypothalamic neuroendocrine function compared to non-stressed controls. Stressed mice exhibited elevated baseline concentrations of corticosterone that remained high at least 6 days after the final exposure to stress. Whereas progesterone concentrations were reduced by stress early in pregnancy, stressed mice recovered typical progesterone secretion during late gestation. These early, stressful experiences resulted in persistent developmental delays, reduced embryo weight, and abnormal placental histology. Significantly, a small percentage of TIDA cells receive close contacts from RFRP-3 axons, providing a mechanism for the control of prolactin secretion by stress. However, contrary to expectation, the percentage of TIDA neurons receiving input from RFRP-3 cells was not impacted by stress. Together, these findings identify a potential pathway of control for the impact of stress on neuroendocrine factors critical to pregnancy success, although further work using more sensitive approaches is needed to examine the putative role of RFRP-3 on stress-induced pregnancy outcomes.

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Effects of Fusarium verticillioides and Lactobacillus Strains Inoculation on Growth and Antioxidant Enzymes Activity of Zea mays Plants

Journal of Horticultural Research ◽

10.1515/johr-2017-0015 ◽

2017 ◽

Vol 25 (2) ◽

pp. 67-74 ◽

Cited By ~ 1

Author(s):

Zohreh Akhavan Kharazian ◽

Mahnaz Aghdasi ◽

Gholamreza Salehi Jouzan ◽

Majid Zamani

Keyword(s):

Enzyme Activity ◽

Antioxidant Enzymes ◽

Growth Parameters ◽

Fusarium Verticillioides ◽

Maize Seedlings ◽

Lactobacillus Pentosus ◽

Enzymes Activity ◽

Maize Plants ◽

The Impact

Abstract The current research based on greenhouse experiment evaluates the impact of the Lactobacillus strains (Lactobacillus plantarum, Lactobacillus paralimentaris, Lactobacillus fermentum, Lactobacillus pentosus, and Lactobacillus buchneri) previously isolated from maize silage on the Fusarium verticillioides-infected maize plants. The growth parameters as well as catalase, superoxide dismutase, ascorbate peroxidase, and peroxidase antioxidant enzymes activity were investigated in one-month old seedlings, after inoculations with Fusarium or co-inoculations with Fusarium and the Lactobacillus strains. Application of Lactobacillus strains in maize seedlings significantly enhanced the plant growth and biomass. The best effect was observed when the L. buchneri was applied. It was revealed that inoculation with Fusarium stimulated antioxidant enzyme activity and co-inoculation with Lactobacillus strains reduced the enzyme activity, compared to Fusarium treatment alone. This is the first report that revealed the bioprotective role of Lactobacillus strains against F. verticillioides.

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Assessment of the role and significance of the cost formation process in the activities of organizations

LAPLAGE EM REVISTA ◽

10.24115/s2446-622020206extra-c664p.314-319 ◽

2020 ◽

Vol 6 (Extra-C) ◽

pp. 314-319

Author(s):

Dmitry A. Gura ◽

Aleksandr N. Sekisov ◽

Oksana A. Kuznetsova ◽

Victoria I. Kalombo Mulamba ◽

Evgeniya S. Tishchenko

Keyword(s):

Comparative Analysis ◽

Production Systems ◽

Cost Effective ◽

Production Costs ◽

Limited Resources ◽

Economic Activities ◽

Effective Mechanism ◽

The Cost ◽

The Impact

The article presents a comparative analysis of various approaches to the formation of production costs. The study estimates the role of costs in the production and economic activities of companies in a market environment. At the same time, the assessment considered the impact of the problem of limited resources on the activities of production systems, which is possible to solve only with the active use of alternative ways in organizing the functioning of the cost-effective mechanism of enterprises.

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The Role of GI Products or Local Products in the Environment—Consumer Awareness and Preferences in Albania, Bulgaria and Poland

Sustainability ◽

10.3390/su14010004 ◽

2021 ◽

Vol 14 (1) ◽

pp. 4

Author(s):

Etleva Muça ◽

Iwona Pomianek ◽

Mariya Peneva

Keyword(s):

Rural Communities ◽

Online Survey ◽

Driving Forces ◽

Production Systems ◽

Sustainable Consumption ◽

Premium Price ◽

Nature Preservation ◽

And Behavior ◽

The Impact

The main goal of this paper is to measure and compare the awareness and preferences of consumers in relation to local products in three countries: Albania, Bulgaria, and Poland. (1) Background: The analysis focused on consumer choices when presented with local products, specifically knowledge as to their environmentally friendly status. The study was evoked by the need to recognize and evaluate changes in consumer behavior as a result of the pandemic and the global challenges related to climate change and the widespread call for nature preservation. (2) Methods: An online survey was conducted with 300 respondents from Poland, 262 from Albania, and 250 from Bulgaria. Statistical analysis was applied. (3) Results: The study answered research questions about consumer readiness to pay a premium price and awareness about the impact of regional products on the environment and livelihood of rural communities. (4) Conclusions: The study proved that consumer perceptions and behavior are influenced by a variety of factors and driving forces in the three countries surveyed depending on socioeconomic characteristics and relevant policies. COVID-19 accelerated the demand for products derived from nature-friendly production systems. Products with geographical indication (GI products) are a better choice from the perspective of sustainable consumption.

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