The Effect of Silver Nanoparticles on the Healing Process of Excisional Skin Wounds in Adult Albino Rats: Histological and Immunohistochemical Study

QJM ◽  
2020 ◽  
Vol 113 (Supplement_1) ◽  
Author(s):  
M Elashry ◽  
I A Baheieldin ◽  
A S S Yonis ◽  
N E Elnefiawy ◽  
Y R A Ahmad ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Image Analysis ◽  
Blood Vessels ◽  
Granulation Tissue ◽  
Healing Process ◽  
Treated Group ◽  
Collagen Fibers ◽  
Albino Rats ◽  
Skin Wounds ◽  
Silver Sulphadiazine

Abstract Background The ultimate goal for wound healing is a speedy recovery with minimal scarring. Different approaches have been used for achieving this goal such as antimicrobials and cell growth promoting agents. Recently, silver nanoparticles (AgNps) have become of interest in biomedical applications, because of their antibacterial, antifungal, antiviral and anti-inflammatory activity. Aim was to investigate the role of AgNps on the healing process of excisional skin wounds in adult male albino rats. Material & Methods 72 male albino rats weighing 200 g were obtained from research center, Ain Shams Faculty of Medicine and subjected to excisional skin wounds 1.5 cm in diameter on their mid-backs. Then rats were divided into 4 groups 18 rats each; control (group I) skin wounds were left to heal naturally. Other wounds were topically treated once daily with methylcellulose hydrogel (group II), silver sulphadiazine (group III), and AgNps in methylcellulose hydrogel (group IV). For each group 6 rats were sacrificed on each of the following days post-wounding; 3rd, 7th and 14th. Skin specimens from all groups were fixed in 10% neutral formalin & processed for paraffin sections which were stained with Hx & E, Mallory’s Trichrome and vascular endothelial growth factor (VEGF). Using image analysis software, number of (macrophages, newly formed blood vessels & fibroblasts) and area % of collagen fibers deposition were assessed and statistically analyzed. Results Control and methylcellulose hydrogel groups revealed almost similar results. Re-epithelialization started on day 3 post-wounding only in AgNps treated group and on day 7 in all other groups. Epithelialization was complete on day 14 only in AgNps treated group and the dermis was mostly regenerated with the appearance of skin appendages. Components of the granulation tissue varied considerably among the studied groups and across the three time points. In Hx & E stained sections the number of macrophages was highest in AgNps treated group on day 3 post-wounding then was the lowest on days 7 and 14 compared with other groups as confirmed by image analysis. Neovascularization started on day 3 in all groups but was prominent on AgNps treated group which showed high density on day 7 as proved statistically and by results of VEGF immune staining. The number of the newly formed blood vessels declined on day 14 in AgNps treated group and remained high in silver sulphadiazine group and showed maximum density in control groups. Statistical analysis proved that the number of fibroblasts in AgNps group was maximum on day 7 & decreased to minimum on day 14 compared with all other groups. In Mallory’s trichrome stained sections the area percent of collagen fibers deposition in the granulation tissue was maximum in AgNps treated group across all periods of the study compared to other studied groups. Conclusion AgNps showed advantage of rapid healing of excisional skin wounds in rats compared with traditional methods of treatment. It might be the treatment of choice particularly for high risk patients.

scholarly journals Ascorbic acid for the healing of skin wounds in rats

2009 ◽  
Vol 69 (4) ◽  
pp. 1195-1201 ◽  
Author(s):  
CC. Lima ◽  
APC. Pereira ◽  
JRF. Silva ◽  
LS. Oliveira ◽  
MCC. Resck ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Healing Process ◽  
Collagen Fibre ◽  
Experimental Period ◽  
Treated Group ◽  
Healing Time ◽  
Skin Wound ◽  
Control Group ◽  
Skin Wounds ◽  
Skin Repair

BACKGROUND: Healing is a complex process that involves cellular and biochemical events. Several medicines have been used in order to shorten healing time and avoid aesthetic damage. OBJECTIVE: to verify the topical effect of ascorbic acid for the healing of rats' skin wounds through the number of macrophages, new vessels and fibroblast verifications in the experimental period; and analyse the thickness and the collagen fibre organization in the injured tissue. METHODS: Male Rattus norvegicus weighing 270 ± 30 g were used. After thionembutal anesthesia, 15 mm transversal incisions were made in the animals' cervical backs. They were divided into two groups: Control Group (CG, n = 12) - skin wound cleaned with water and soap daily; Treated Group (TG, n = 12) - skin wound cleaned daily and treated with ascorbic acid cream (10%). Samples of skin were collected on the 3rd, 7th and 14th days. The sections were stained with hematoxylin-eosin and picrosirius red for morphologic analysis. The images were obtained and analysed by a Digital Analyser System. RESULTS: The ascorbic acid acted on every stage of the healing process. It reduced the number of macrophages, increased the proliferation of fibroblasts and new vessels, and stimulated the synthesis of thicker and more organized collagen fibres in the wounds when compared to CG. CONCLUSION: Ascorbic acid was shown to have anti-inflammatory and healing effects, guaranteeing a suiTable environment and conditions for faster skin repair.

scholarly journals Biocompatible Nanocomposite Implant with Silver Nanoparticles for Otology—In Vivo Evaluation

Nanomaterials ◽  
2018 ◽  
Vol 8 (10) ◽  
pp. 764 ◽  
Author(s):  
Magdalena Ziąbka ◽  
Elżbieta Menaszek ◽  
Jacek Tarasiuk ◽  
Sebastian Wroński
Keyword(s):  
Silver Nanoparticles ◽  
Middle Ear ◽  
Granulation Tissue ◽  
Ex Vivo ◽  
Healing Process ◽  
Silver Ions ◽  
In Vivo Studies ◽  
Surrounding Tissue ◽  
Injection Moulding Process

The aim of this work was to investigate of biocompatibility of polymeric implants modified with silver nanoparticles (AgNPs). Middle ear prostheses (otoimplants) made of the (poly)acrylonitrile butadiene styrene (ABS) and ABS modified with silver nanoparticles were prepared through extrusion and injection moulding process. The obtained prostheses were characterized by SEM-EDX, micro-CT and mechanical tests, confirming their proper shape, good AgNPs homogenization and mechanical parameters stability. The biocompatibility of the implants was evaluated in vivo on rats, after 4, 12, 24 and 48 weeks of implantation. The tissue-healing process and cytotoxicity of the implants were evaluated on the basis of microscopic observations of the materials morphology after histochemical staining with cytochrome c oxidase (OCC) and acid phosphatase (AP), as well as via micro-tomography (ex vivo). The in vivo studies confirmed biocompatibility of the implants in the surrounding tissue environment. Both the pure ABS and nanosilver-modified ABS implants exhibited a distinct decrease in the area of granulation tissue which was replaced with the regenerating muscle tissue. Moreover, a slightly smaller area of granulation tissue was observed in the surroundings of the silver-doped prosthesis than in the case of pure ABS prosthesis. The kinetics of silver ions releasing from implants was investigated by ICP-MS spectrometry. The measurement confirmed that concentration of the silver ions increased within the implant’s immersion period. Our results showed that middle ear implant with the nanoscale modification is biocompatible and might be used in ossicular reconstruction.

scholarly journals Analysis of the influence of low-power HeNe laser on the healing of skin wounds in diabetic and non-diabetic rats

2006 ◽  
Vol 21 (3) ◽  
pp. 177-183 ◽  
Author(s):  
Paulo de Tarso Camillo de Carvalho ◽  
Nilton Mazzer ◽  
Filipe Abdalla dos Reis ◽  
Ana Carulina Guimarães Belchior ◽  
Iandara Schettert Silva
Keyword(s):  
Low Power ◽  
Treated Group ◽  
Diabetic Rats ◽  
Collagen Fibers ◽  
Diabetic Group ◽  
Skin Wounds ◽  
Significance Level ◽  
Hene Laser ◽  
Dorsal Vein ◽  
Male Wistar Rats

PURPOSE: To study the influence of HeNe laser irradiation on the collagen percentage in surgically-induced skin wounds in rats with and without alloxan-induced diabetes, by morphometric analysis of collagen fibers. METHODS: 48 male Wistar rats were used, divided into groups: laser-treated diabetic (group 1); untreated diabetic (group 2); treated non-diabetic (group 3); and untreated non-diabetic (group 4). For groups 1 and 2, diabetes was induced by intravenous injection of alloxan (2,4,5,6-tetraoxypyrimidine; 5,6-dioxyuracil; Sigma), into the dorsal vein of the penis, at a rate of 0.1 ml of solution per 100 g of body weight. A wound was made on the back of all the animals. Groups 1 and 3 were treated with HeNe laser (4 J/cm²) for 60 s. One animal from each group was sacrificed on the 3rd, 7th and 14th days after wounding. Samples were taken, embedded in paraffin, stained with hematoxylin-eosin and Masson's trichrome, and morphometrically analyzed using the Imagelab software. The percentages of collagen fibers were determined from the samples from the euthanasia animals. The data were treated statistically using analysis of variance (ANOVA) and the Student t and Kruskal-Wallis tests. The significance level was set at 0.05 or 5%. RESULTS: The results obtained from the samples taken on the third, seventh and fourteenth days after wounding demonstrated that the laser-treated group presented a statistically significant (p<0.05) greater mean quantity of collagen fibers than in the non-treated group, both for diabetic rats (p = 0.0104) and for non-diabetic rats (p = 0.039). CONCLUSION: The low-power laser (632.8 nm) was shown to be capable of influencing the collagen percentage in skin wounds by increasing the mean quantity of collagen fibers, both for the diabetic and for the non-diabetic group.

Anti-diabetic Efficacy of Silver Nanoparticles Biosynthe-sized from Marine Red Seaweed Halymenia porphyroides Boergesen on Alloxan Stimulated Hyperglycemic Activity in Rats

2021 ◽  
Vol 14 (5) ◽  
pp. 5639-5646
Author(s):  
Vishnu Kiran Manam ◽  
Subbaiah Murugesan
Keyword(s):  
Silver Nanoparticles ◽  
Body Weight ◽  
Treatment Group ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Treated Group ◽  
The Body ◽  
Albino Rats ◽  
Red Seaweed

The assessment of silver nanoparticles biosynthesized and characterized using UV-Spec, FTIR, XRD, TGA, SEM, TEM from marine red seaweed Halymenia porphyroides have been evaluated for its anti-hyperglycemic activity in vivo. The anti-diabetic efficacy of the biosynthesized silver nanoparticles from marine red seaweed Halymenia porphyroides was studied by chemically inducing diabetes in the experimental Wistar albino rats through Alloxan monohydrate, which ultimately results in hyperglycemia at a dosage of 50 mg/kg body weight given orally for about 28 days. The outcome of the results was estimated by various biochemical parameters from the treatment group with silver nanoparticle (50 mg/Kg i.p) biosynthesized from Halymenia porphyroides. The anti-diabetic efficacy of the treatment group showed a decrease in the levels of blood glucose levels, total cholesterol, triglycerides, low-density lipoprotein, and phospholipids whereas the body weight and HDL increase was observed. The histopathological evaluation of the pancreas of the treated group of animals revealed the restoration and regeneration of β-cells of the pancreas with moderate swelling as compared to that of the chemically induced alloxan diabetic group of animals.   

scholarly journals Alpha-Lipoic Acid and Ginkgo Biloba Ameliorate Testicular Dysfunctions Induced by Silver Nanoparticles in Rats

2021 ◽  
Author(s):  
Hossam G. TOHAMY ◽  
mohamed lebda ◽  
kadry sadek ◽  
mohamed elfeky ◽  
yasser elsayed ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Mrna Expression ◽  
Lipoic Acid ◽  
Ginkgo Biloba ◽  
Transforming Growth Factor ◽  
Serum Testosterone ◽  
Treated Group ◽  
Serum Testosterone Level ◽  
Albino Rats ◽  
Alpha Lipoic Acid

Abstract Silver nanoparticles (AgNPs) are more commonly utilised in medicine, however they have negative effects on the majority of organs, including the reproductive system. AgNPs were reported to be able to reach the testes due to their small size, which allows them to pass through blood testicular barriers. The goal of this study was to see if LA (alpha lipoic acid) or GB (ginkgo biloba) might protect adult rat testes after intraperitoneal injection of AgNPs. Forty male healthy adult Wister albino rats were randomly assigned to four groups (10 rats each); control, AgNPs-intoxicated group intraperitoneally injected AgNPs 50 mg/kg b.w, 3 times a week, LA + AgNPs group intoxicated with AgNPs and orally gavaged with 100 mg LA/kg b.w, and GB + AgNPs group injected with AgNPs and orally given GB extract 120 mg/kg b.w were continued for 30 consecutive days. Biochemical changes in testicular tissue (testosterone, ACP, and Prostatic acid phosphatase), oxidative indices in testicles tissues, mRNA expression of pro-apoptotic (BAX) and anti-apoptotic (BCL-2) biomarkers, histological, and immunohistochemical changes were studied. Significant decrease in serum testosterone level and elevation in ACP and PACP enzyme activity in AgNPs treated group than in the control. In addition, lowering in tGSH, GSH GR, GPx and elevation MDA and GSSG were observed in AgNPs treated group than control. Decreasing in mRNA expression thirodexin-1 (Txn-1), transforming growth factor-1β (TGF-1β), anti-apoptic (BCL-2) and elevation the expression of proapoptic biomarkers (BAX) in the testis homogenates of rats exposed to silver nanoparticles. Strong positive action to BAX and lowering the action of Ki-67antibody were observed. Because of their antioxidant, anti-inflammatory, and anti-apoptotic properties, co-treatment with LA or GB may be beneficial in reducing the harmful effects of AgNPs on the testicles.

scholarly journals The effect of hyaluronic acid on the skin healing in rats

2019 ◽  
Vol 10 (2) ◽  
pp. 65-75
Author(s):  
Livia Medeiros Soares Celani ◽  
Isadora Soares Lopes ◽  
Aldo Cunha Medeiros
Keyword(s):  
Wound Healing ◽  
Hyaluronic Acid ◽  
Granulation Tissue ◽  
Wound Repair ◽  
Histopathological Examination ◽  
Healing Process ◽  
Control Group ◽  
Skin Wounds ◽  
Collagen Deposition ◽  
Morphological Differences

Purpose: The objective of this study was to examine the effect of topical hyaluronic acid (HA) treatment on wound healing in rats. Methods: Wistar rats were randomly selected and allocated into 2 groups, with six rats each, all of them submitted to skin lesions (open wound with 5mm diameter). Group 1 – rats treated with topical 0,9% saline application in skin wounds. Group 2 – rats treated with topical hyaluronic acid 0,4% on skin wounds once a day for 7 days. After anesthesia on the 7th postoperative day, a biopsy of the skin dorsal wound area was performed for histopathological examination. A classification system based on the degree of reepithelization, granulation tissue formation and collagen organization were used to relate the morphological differences in wound healing. Results: Rats from control group treated with saline solution, on day 7, presented a general impairment of healing process, marked by an incomplete reepithelization (score 2,67 ± 0,4), a persistent exudation, fibrin, reduced accumulation of granulation tissue (score 2,2 ± 0,5) and a slight collagen deposition (score 2,43 ± 0,5), compared with the HA group. On the other side, morphological differences were visible on the evolution of wound repair process in rats treated with HA with a significant reduction in fibrinous exudation, epidermal lining reconstitution by complete reepithelization (score 3,8 ± 0,6), an increased accumulation of granulation tissue at wound edge and bed (score 3,7 ± 1,1), moderate collagen deposition (3,8 ± 0,7). The respective differences between the groups were significant (p<0,05). Conclusions: Based on these findings, we may conclude that HA treatment contributes to improve healing of open wounds.

scholarly journals Effect of Lyophilized Growth Factors (LGF) Derived from Equine Platelets on Experimentally Induced Skin Wound Healing in Mongrel Dogs

2021 ◽  
Vol 10 (2) ◽  
pp. 75-82
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Healing Process ◽  
Treated Group ◽  
Skin Wound ◽  
Skin Wounds ◽  
Skin Wound Healing ◽  
Thickness Skin ◽  
The Right ◽  
Tgf Β1

Lyophilized growth factor (LGF) is a novel advanced platelets rich protein growth factor. It has been successfully applied in various fields of regenerative medicine including management of chronic and non-healing wounds and ulcers. Our study aimed to evaluate the effect of subcutaneous infiltration of equine platelets derived lyophilized growth factors on healing of induced full thickness skin wound in dogs. Four healthy mongrel dogs of different ages were used in the current work. Three bilateral critical sized skin wounds were done on the back of each dog. After 24 hours, the right-side wounds were injected in 4 cardinal points around each wound by Saline/lidocaine (control wounds) and simultaneously reconstituted LGF was subcutaneously injected around the left side wounds (LGF treated wounds). Wound contraction was monitored physically and histopathologically. The expression of TGF-β1 and NF-κB was evaluated in wound specimens of both groups. A significant reduction in wound size was recorded in LGF treated group compared to saline treated group. The histopathological scoring of the healing progress revealed significant increase in the degree of re-epithelization bridging the wound edges and collagen deposition in LGF-treated wounds compared to control non-treated wounds along the experimental periods. Additionally, the expression of TGF-β1 and NF-κB showed significant elevation in LGF-treated wounds compared with their expression in control wounds. In conclusion, LGF therapy could be a superior candidate as a regenerative therapy in skin wounds that can positively impact healing process of the cutaneous wounds.

Perivascular tissue factor is down-regulated following cutaneous wounding: implications for bleeding in hemophilia

Blood ◽  
2008 ◽  
Vol 111 (4) ◽  
pp. 2046-2048 ◽  
Author(s):  
Anna G. McDonald ◽  
Katie Yang ◽  
Harold R. Roberts ◽  
Dougald M. Monroe ◽  
Maureane Hoffman
Keyword(s):  
Wound Healing ◽  
Blood Vessels ◽  
Tissue Factor ◽  
Inflammatory Cytokines ◽  
Granulation Tissue ◽  
Thrombin Generation ◽  
Hemophilia B ◽  
Skin Wounds ◽  
Wild Type ◽  
Healing Of Skin Wounds

Healing of skin wounds is delayed in hemophilia B (HB) mice. HB mice do not bleed excessively at wounding, yet rebleed hours to days later. Tissue factor (TF) expression is up-regulated by inflammatory cytokines and has been linked to angiogenesis. We hypothesized that impaired thrombin generation in HB leads to impaired TF expression following injury. Punch biopsies were placed on wild-type (WT) and HB mice. Tissues from wound sites were immunostained for TF. Blood vessels are normally surrounded by a coat of pericytes expressing TF. Surprisingly, within a day after wounding TF disappeared from around nearby vessels; returning after 8 days in WT and 10 days in HB mice. The granulation tissue filling the wound during healing also lacked TF around angiogenic vessels. Thus, perivascular TF expression is down-regulated during wound healing. This may prevent thrombosis of neovessels during angiogenesis but renders hemophiliacs vulnerable to hemorrhage during healing.

Impact of cefepime on hematological, immunological and oxidant/antioxidant parameters in rats experimentally infected with E. coli ATCC 25922

2020 ◽  
Vol 21 (1) ◽  
pp. 36-45
Author(s):  
Huda Elbaz ◽  
Mohamed Hamed ◽  
Fatma Abdelhamid ◽  
Osama Abdalla
Keyword(s):  
Liver Function ◽  
Oxidative Damage ◽  
Hemoglobin Concentration ◽  
Treated Group ◽  
Serum Glucose ◽  
Infected Group ◽  
Fixed Dose ◽  
Albino Rats ◽  
Immunological Parameters ◽  
Antioxidant Parameters

Objective: To evaluate the effect of cefepime on hematological changes, immunological disorders and hepatic oxidative damage in rats experimentally infected with E.coli ATCC 25922. Design: Randomized controlled experimental study. Animals: Thirty-two adult male albino rats weighting150-200 g. Procedures: Rats used for this study were randomly assigned into 4 equal groups: the control one, E.coli infected group (1×108CFU/I/P/once), the cefepime treated group (45 mg/kg bw/I/M/day) for 5 days and the E.coli infected group that treated with cefepime 24h after bacterial inoculation as previously described. Hematological and immunological parameters, liver function biomarkers and hepatic oxidative stress and antioxidant markers were determined. Results: Our result revealed that E.coli infection induced a significant elevation in the erythrocytes count, hemoglobin concentration, PCV% and total leukocytic count (TLC) (P < 0.05). In the same respect, liver function biomarkers, serum glucose, total cholesterol, and triglyceride levels as well hepatic malondialdehyde (MDA), nitric oxide (NO), TNF-α, IL-10, and lysozyme activity were significantly increased compared to the control rats (P < 0.05). In contrast, hepatic reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) were decreased significantly (P < 0.05). Cefepime treatment in E.coli + CFPM group reduced the elevated eythrogram, TLC and liver function biomarkers. Cefepime also ameliorated the oxidative damage and inflammatory response induced by E.coli infection. Conclusion and clinical relevance: Cefepime is safe when administered in a fixed-dose and possess antioxidant that contributes to improve efficacy against adverse effect induced by E.coli ATCC 25922 infection.

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