scholarly journals Intranasal oxytocin treatment on the day of weaning does not decrease walking behavior or improve plasma metabolites in beef calves placed on pasture

Author(s):  
K R Nickles ◽  
A E Relling ◽  
A J Parker

Abstract The purpose of this study was to evaluate the effect that intranasal oxytocin administered at abrupt weaning (day 0) had on weaning stress behaviors such as walking distance and time devoted to walking, calf body weight, and plasma non-esterified fatty acids (NEFA), β-hydroxybutyrate (β-HB), and cortisol. Twenty Simmental × Angus heifer calves were randomly assigned to one of two treatments, intranasal oxytocin (OXT; n = 10) or intranasal saline (CON; n = 10). All calves were given the respective intranasal treatment on the day of weaning (day 0), and then placed on pasture together. Calves were weighed and a blood sample was obtained on days 0, 1, 7, and 14 post-weaning. Blood samples were subsequently used to quantify plasma NEFA, β-HB, and cortisol concentrations. All calves in both treatment groups were fitted with an individual global positioning system (GPS) that recorded calf location every ten seconds for a 16 hour period on days 0, 7, and 14 to quantify and evaluate walking behaviors. There was no treatment × day effect for distance walked (P = 0.82), walking time (P = 0.80), non-walking time (P = 0.88), area utilization index (P = 0.84), calf body weight (P = 0.82), average daily gain (P = 0.54), NEFA (P = 0.22), or cortisol concentrations (P = 0.32). There was a tendency for a treatment × day effect observed for average walking speed (P = 0.09) and β-HB (P = 0.10), such that calves in the CON treatment tended to have lesser average walking speeds on day 14 and tended to have greater β-HB concentration after weaning. There was a treatment effect (P = 0.02) for NEFA concentrations, with the CON calves having a greater plasma NEFA concentration throughout the study compared with OXT calves. These data imply that OXT calves may have had differing metabolic responses immediately after weaning that could have altered the mobilization of NEFA, but this change was not substantial enough to impact body weights or walking behaviors.

Author(s):  
Esteban M Galarza ◽  
Raul M Lizzarraga ◽  
Guillermo A Mattioli ◽  
Anthony J Parker ◽  
Alejandro E Relling

Abstract Weaning and transport represent a high stress time for calves. Preconditioning (PC) by weaning before the transport separate these stressors. The stressors generate oxidative stress, which can be reduced by mineral and vitamin supplementation (MVS) with an antioxidant capacity. Our objective was to evaluate the effect of PC and MVS on performance of steers. The experiment used a 2 × 2 factorial arrangement design, considering a 26-d PC treatment from weaning to transport to the feedlot (d 0); and injectable MVS on d -45, -26, and 0. The MVS consisted of Cu, Zn, Mn, Se, vitamin E (0.2, 0.8, 0.2, 0.1, and 1 mg/kg body weight (BW), respectively), and vitamin A (1190 IU/kg). Sixty Angus-crossbred steers (186.4 ± 27.6 kg) were randomly assigned to the four treatments (MVS+PC; N+PC; MVS+N; N+N; n = 15/treatment). Body weight (BW) was recorded on d -45, -26, 0, 8, 15, and 29. On d 0, an additional BW was taken 30 minutes after the 5-hour transportation (d 0.5). Between d 0 to 29, dry matter intake (DMI) and average daily gain (ADG) to DMI ratio (G:F) were measured. Between d -26 to 29 plasma concentrations of glucose, free fatty acids (NEFA), cortisol, insulin, total antioxidant status (TAS), and thiobarbituric acid-reactive substances (TBARS) were evaluated. Data were analyzed using the MIXED procedure of SAS with repeated measures, using treatment, time, and treatment × time as fixed effects, and steer as a random effect. Between d -26 to 0, there was an interaction of MVS × PC (P ˂ 0.01) for ADG. From d -26 to 0, N+N and N+PC had the greatest and lesser ADG, respectively. On d 0.5, no-PC steers tended to lose BW while the PC steers tended to gain BW (P = 0.09). In the period d 0 to 8, there were no differences (P ≥ 0.27) in DMI, but the PC steers had greater G:F and ADG (P < 0.01) compared with no-PC steers. Plasma NEFA concentration on d 0 was affected by MVS×PC (P < 0.01), because MVS decreased plasma NEFA concentration in no-PC steers, but it increased in the PC steers. Plasma concentrations of glucose, insulin, and cortisol did not differ among treatments (P ≥ 0.23). There was a MVS×PC interaction (P = 0.09) for TAS on d 0; N+N had the greatest and MVS+N had the lowest TAS concentrations. In conclusion, a 26-d PC decreased steers BW compared with no-PC steers. The BW loss during preconditioning was not recovered 29-d after feedlot entry. Despite this BW loss, MVS treatment decreased BW loss in the steers allocated to PC treatment on the day of transport.


2022 ◽  
Vol 18 (1) ◽  
Author(s):  
Min-Jin Kwak ◽  
Sun-Woo Choi ◽  
Yong-Soon Choi ◽  
Hanbae Lee ◽  
Kwang-Youn Whang

Abstract Background In animals, weaning stress is the first and most critical stress. Weaning can negatively affect the growth performance of animals physically, psychologically, and pathologically. Our previous studies on the HT-29 cell line and early-weaned rats demonstrated that adequate sophorolipid (SPL) supplementation in feed could enhance the mucin-producing and wound healing capacities of the gut defense system by modulating gut microbiota. Methods We conducted an experiment with one hundred forty 21-day-old early weaned piglets (L x Y x D). They were allocated into 4 treatment and 7 replications (4 pigs per pen) according to their initial body weight. Body weight and feed intake were measured biweekly during experimental period. After 6 weeks, 28 pigs were randomly selected and sacrificed to collect plasma, jejunum, and cecal content samples. Results Dietary SPL supplementation at 5 and 10 mg/kg quadratically increased the average daily gain during the experimental period in the treatment groups when compared with the control group. The albumin levels of piglets fed with the SPL supplemented diet were downregulated to the normal range. Moreover, in feed, SPL supplementation at 5 and 10 mg/kg improved jejunal histological indices and gene expression levels related to mucin secretion and local inflammation markers. Consistent with these results, adequate SPL supplementation (5 and 10 mg/kg) increased the population of Prevotella, a beneficial bacterium, and its short-chain fatty acid production in the ceca of piglets. Conclusions The occurrence of diarrhea after weaning in piglets could be reduced by feeding a 10 ppm of SPL supplemented diet which improves the gut defense system by improving the microbial population and enhancing mucin layer integrity.


2018 ◽  
Vol 3 (7) ◽  
pp. 173 ◽  
Author(s):  
Diyanah Inani Azmi ◽  
Hafazah Abdul Karim ◽  
Mohd Zamreen Mohd Amin

Walking behavior can be considered as one of the important factors in measuring the walkability level in neighborhood. Walking behavior has close interrelationships with walkability. This paper is based on a comparative study of walking behaviour of residents between urban and rural neighborhood. The method used in this study is known as walking distance test and the data were analyzed using the comparative approach. The findings show that there are slight differences of walking speed, walking time and walking speed between residents in the urban and rural neighborhood area. Keywords: Walking behaviour; rural residents; urban residents; walking distance eISSN 2514-7528 © 2018. The Authors. Published for AMER ABRA cE-Bs by e-International Publishing House, Ltd., UK. This is an open-access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Peer–review under responsibility of AMER (Association of Malaysian Environment-Behaviour Researchers), ABRA (Association of Behavioural Researchers on Asians) and cE-Bs (Centre for Environment-Behaviour Studies), Faculty of Architecture, Planning & Surveying, Universiti Teknologi MARA, Malaysia.  


Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1543
Author(s):  
Sang-Ho Moon ◽  
Yeong Sik Yun ◽  
Na Yeon Kim ◽  
Sanguk Chung ◽  
Qi Man Zhang ◽  
...  

Twelve adult (10 months old) castrated Korean black goats, with an average initial body weight of 24.98 ± 3.7 kg, were used in this experiment to determine their maintenance energy requirements. Dry matter intakes (g/d, p = 0.945) were not affected by energy levels, but metabolic energy intake (kcal/d, p < 0.002) and average daily gain (g/d, p < 0.001) were significantly increased at higher energy levels. Nutrient digestibility was similar in the treatments, but crude fat digestibility increased with the addition of protective fat powder (p = 0.001). The energy required for fattening the castrated Korean black goats was estimated using the correlation between metabolic energy intake per dietary body weight and average daily gain per dietary body weight. The Y-axis intercept value was calculated to be 108.76 kcal/kg BW0.75 (p < 0.05, r2 = 0.6036), which was the metabolic energy requirement for maintaining the lives of the fattening Korean black goats. The estimated energy requirements of the black goat can improve specification techniques, such as the energy level and the amount of feed supply required for domestic black goats.


Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 218
Author(s):  
Yousef Abbaslou ◽  
Davood Zahmatkesh ◽  
Ehsan Mahjoubi ◽  
Mehdi Hossein Yazdi ◽  
Hamed Beiranvand ◽  
...  

The positive effects of nucleotide (NU) supplementation in milk replacer have been elucidated in infants and in dairy calves; however, NU addition to whole milk has not been evaluated previously. This study aimed to assess NU supplementation in the whole milk on calf growth and health. Thirty Holstein calves (body weight: 39.1 ± 1.0 kg; 3 d after birth) were randomly assigned to the following treatments: whole milk without any supplementation (NU0), whole milk + 0.5 g/d added a NU-containing supplement to whole milk (NUCS0.5), and whole milk + 1 g/d added a NU-containing supplement to whole milk (NUCS1). Calves were weaned at d 55 and stayed on study until d 75. Calves had free access to feed and water throughout the study. Dry matter intakes (DMI) were similar among treatments (p > 0.05) during the pre-weaning period; however, increasing NU resulted in a linear (p < 0.05) increase in DMI during the post weaning period (2158, 2432, and 2518 g/d for NU0, NUCS0.5, and NUCS1, respectively). Treatments did not affect body weight (BW) at the first and second month of study, but final BW linearly increased as NU was added (87.1, 90.6, and 95.4 kg for NU0, NUCS0.5, and NUCS1, respectively). Neither pre-weaning average daily gain nor post-weaning average daily gain was affected by treatments; accordingly, feed efficiency was similar among treatment groups. Days with loose fecal score were linearly decreased as NU was added to whole milk during the first month of life, while the fecal score did not differ among treatments until the end of the study. No difference was observed in the skeletal growth of calves in the current study. Therefore, it can be concluded that NU supplementation in the whole milk has some beneficial effects on calf performance in terms of final BW, post-weaning DMI, and less days with loose feces.


2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 74-74
Author(s):  
Ellen M Herring ◽  
Elizabeth B Kegley ◽  
Ken Coffey ◽  
Shane Gadberry ◽  
Jeremy G Powell ◽  
...  

Abstract This research evaluated the effects of ensiled edamame soybean processing waste on ruminant intake and total tract digestibility. Waste from processing stored edamame was obtained (3 trips) and wilted (28%, 25%, and 37% DM), then treated with or without inoculant (Lactobacillus buchneri) and ensiled in 167 L plastic barrels (2 or 3 barrels/treatment from each trip) for 72 (Trip 1) or 69 (Trip 2 and 3) days. Pregnant ewes (n = 18; body weight = 55.5 ± 1.2 kg; 2 to 3 years old) were blocked by body weight and assigned randomly within a block to treatments within a trip, then each ewe was assigned to a barrel of silage. Ewes were housed individually in 1 × 1.5-m pens and offered silage for a 10-day adaptation period followed by 7 days of total feces and urine collection. Ewes were offered silage to allow for 10% orts and were offered 0.2% of their body weight of soyhulls and 32 g of mineral supplement/day to meet their predicted nutrient requirements for gestating ewes. Ewes consumed 1,616 ± 54 g DM/day (X ± SEM) or 2.9 ± 0.12% of their body weight and there was no effect (P ≥ 0.85) of inoculant treatment on DM intake (g/day or % of body weight). Dry matter digestibility was not affected (P = 0.98) by inoculant and averaged 55.7 ± 0.66%. Ewe average daily gain for the 17-day trial tended to be greater (P = 0.08) for the ewes offered the silage without inoculant (0.18 vs. 0.04 kg/d). Ensiling edamame processing waste yielded a feed that ewes consumed in adequate amounts to maintain their body weights over 17 days when also supplemented with soyhulls. The addition of silage inoculant had minimal effects on intake, digestibility, or ewe body weight change.


2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 193-195
Author(s):  
Vetriselvi Sampath ◽  
Hyun Ju Park ◽  
Yong min Kim ◽  
Je Min Ahn ◽  
Inho Kim

Abstract BACKGROUND: A total of one hundred and forty, 28 d-old weaner pigs [Duroc x (Yorkshire x Landrace)] with initial body weight (BW) of 6.56±1.25kg were used in a six-week treatment (7 replicate pens per treatment; barrows, and 2 gilts/pen) to evaluate the effect of low nutrient density diet supplement with probiotic mixture supplementation on the growth performance, nutrient digestibility, fecal microbial, and gas emission of weaner pigs. RESULTS: Pigs fed low-density diet with probiotic mixture supplementation had linearly increased (P = 0.028, 0.014) the body weight (BW) at weeks 3, and 6. Moreover, average daily gain (ADG) was linearly improved (P=0.018, 0.014, 0.014) at week 3, 6, and overall experiment. However, there were no interactive effects found on the nutrient digestibility of dry matter (DM), nitrogen (N) and energy (E) throughout the experiment. Dietary inclusion of low-density diet with probiotic mixture supplementation has improved the fecal lactobacillus counts linearly, but E. coli was unaffected during the trail. On day 42, Ammonium gas emission was significantly decrease in pigs fed a low-density diet with probiotic mixture supplementation. However, H2S, acetic acid, and CO2 were not significantly affected by the probiotic mixture supplementation diet. CONCLUSION: Low-density diet with probiotic mixture supplementation had positively affected the growth performance, fecal microbial, and fecal gas emission on weaner pigs.


2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 399-399
Author(s):  
Ibukun M Ogunade

Abstract This study applied a chemical isotope labeling/liquid chromatography mass spectrometry-based metabolomics technique to determine differences in plasma metabolites containing amine/phenol and carbonyl chemical groups in beef steers with divergent average daily gain (ADG). Thirty-eight Angus crossbred beef steers (21 d post-weaning; 210 ± 12 kg of BW) from a single source were housed in individual slatted floor pens and were fed the same total mixed ration (CP = 14.5% and NEg = 1.10 Mcal/kg) ad libitum for 42 d with free access to water. After 42 days of feeding, the steers were divided into two groups of lowest ADG (LF: n = 8) and highest (HF: n = 8) ADG. Blood samples were taken from both LF and HF steers and were immediately centrifuged to harvest the plasma. The average daily DM intake of the steers in LF and HF were 6.08 kg ± 0.57 and 6.04 kg ± 0.42, respectively, and was similar between the two groups (P = 0.72). The ADG of LF (0.99 kg ± 0.23) was lower (P = 0.01) than that of HF (1.63 kg ± 0.20). A total number of 42 carbonyl-containing metabolites and 229 amine/phenol-containing metabolites were identified in the plasma samples of both groups. No alteration in carbonyl-metabolome was detected. Ten metabolites including 4,6-dihydroxyquinoline, prolyl-valine, prolyl-leucine, prolyl-isoleucine, L-formylkynurenine, pyrocatechol, and histidine were greater in HF steers whereas 8 metabolites including arginine, phenylalanine, guanidoacetic acid, and aspartyl-threonine were greater in LF steers. This study demonstrated that beef steers with divergent ADG had altered plasma amine/phenol metabolome. Notably, plasma concentrations of dipeptides containing branched chain amino acid residues (prolyl-valine, prolyl-leucine, prolyl-isoleucine) and metabolites with anti-inflammatory and reactive oxygen-scavenging properties (4,6-dihydroxyquinoline and L-formylkynurenine) were greater in steers with high ADG.


2018 ◽  
Vol 41 (1) ◽  
pp. 42552
Author(s):  
Fatima Kouri ◽  
Salima Charallah ◽  
Amina Kouri ◽  
Zaina Amirat ◽  
Farida Khammar

 This research aims to assess the performances of lactating Bedouin goat under arid conditions, focusing on the relationship of milk yield with body and udder morphology and growth traits. Fourteen goats and their kids were followed during early lactation (90 days post-partum). Mean daily milk yield was 0.56 kg. Milk fat, proteins, lactose, solids non-fat and minerals were respectively 34.9, 38.9, 48.8, 107 and 6.7 g L-1. Mean kids’ birth weight, adjusted body weight at 90 days of age and average daily gain were respectively 2 kg, 6.6 kg and 51.4 g day-1. Daily milk yield was correlated with goats’ body weight, withers height, rump height, udder width and udder circumference but not with linear udder score and growth performances. These results provide important data about the milk production of Bedouin goat emphasizing its correlation with body and udder morphological traits which can help to elaborate a specific breeding program for this breed. 


1964 ◽  
Vol 6 (3) ◽  
pp. 301-308 ◽  
Author(s):  
Henning E. Nielsen

Sixteen blocks of eight littermate pigs were weaned at 3 weeks of age and each divided into four groups. Various levels of nutrition were applied to the pigs so that the four groups reached 20 kg. at 59, 68, 80 and 91 days of age, respectively.Only small differences were observed between groups in feed conversion ratio to 20 kg.; however, Group 4 required more feed per kg. gain than the three other groups.During the second period (20–90 kg.) the pigs in each group were divided into two sub-groups, which were on a high and a low plane of nutrition respectively. In both sub-groups the type of feeding before 20 kg. influenced the average daily gain and feed conversion ratio. The older the pigs were at 20 kg. the higher the daily gain and the lower the feed conversion ratio in the following period. There was an increase in daily feed intake with increased age at 20 kg. for pigs fed ad lib. during the second period.An increase in age at 20 kg. caused a significant decrease in thickness of backfat, a greater area of eye muscle and a smaller area of fat overlying muscle. For pigs fed ad lib. the area of eye muscle was significantly increased by a higher age at 20 kg. live-weight.


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