scholarly journals Green Fabricated Zinc Oxide Nanoformulated Media Enhanced Callus Induction and Regeneration Dynamics of Panicum virgatum L.

2020 ◽  
Author(s):  
Saima Shafique ◽  
Nyla Jabeen ◽  
Khawaja Shafique Ahmad ◽  
Samra Irum ◽  
Sadaf Anwaar ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Plant Regeneration ◽  
Zinc Oxide Nanoparticles ◽  
Panicum Virgatum ◽  
Regeneration Ability ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Panicum Virgatum L ◽  
High Doses ◽  
Average Size

AbstractThe current study was focused on the usage of bio synthesized zinc oxide nanoparticles to increase the tissue culture efficiency of important forage grass Panicum virgatum. Zinc being a micronutrient enhanced the callogenesis and regeneration efficiency of Panicum virgatum at different concentrations. Here, we synthesized zinc oxide nanoparticles through Cymbopogon citratus leaves extract to evaluate the influence of zinc oxide nanoparticles on the quality of plant regeneration in switchgrass. X-ray diffraction (XRD) and attenuated total reflectance-Fourier transform infrared (ATR-FTIR) validate phase purity of green synthesize Zinc oxide nanoparticles whereas, electron microscopy (SEM) has illustrated the average size of particle 50±4 nm with hexagonal rod like shape. Energy dispersive Xray (EDS) spectra depict major peaks of Zn (92.68%) while minor peaks refer to Oxygen (7.32%). ZnO NPs demonstrate the incredibly promising results against callogenesis. Biosynthesized ZnO NPs at optimum concentration showed very promising effect on plant regeneration ability. Both the explants, seeds and nodes used in study showed dose dependent response and upon high doses exceeding 40 mg/L the results were recorded negative, whereas at 30 mg/L both explants demonstrate 70 % and 76 % regeneration frequency. The results conclude that zinc oxide nanoparticles enhance plant growth and development. Being one of the essential plant nutrients, ZnO has greatly tailored the nutritive properties at nano-scale.

scholarly journals Large Scale Synthesis of Green Synthesized Zinc Oxide Nanoparticles from Banana Peel Extracts and Their Inhibitory Effects against Colletotrichum sp., Isolate KUFC 021, Causal Agent of Anthracnose on Dendrobium Orchid

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Nattanan Panjaworayan T-Thienprasert ◽  
Jiraroj T-Thienprasert ◽  
Jittiporn Ruangtong ◽  
Thitiradsadakorn Jaithon ◽  
Pattana Srifah Huehne ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Large Scale ◽  
Average Diameter ◽  
Banana Peel ◽  
Oxide Nanoparticles ◽  
Inhibitory Effects ◽  
Zno Nps ◽  
Nontarget Organisms ◽  
High Doses

Fungicides have been extensively used to control fungal diseases that affect several crops including ornamental crops. However, concerns have arisen due to a development of fungicide resistance and increasing incidences of fungicide toxicity effects on nontarget organisms. As zinc oxide nanoparticles (ZnO NPs) have demonstrated effective antimicrobial activity, this study is therefore aimed at synthesizing ZnO NPs from banana peels using a green chemistry method in a large scale and determines their physical properties including their inhibitory effects against a plant pathogen fungus causing anthracnose in orchids, Colletotrichum sp. Results from X-ray diffraction and scanning electron microscope indicated that the synthesized ZnO NPs were obtained without other crystalline impurities, and they were spherical in shape with the average diameter of 256 ± 40   nm , respectively. The absorption peak was found to be centered at ~370 nm with the optical band gap value approximately 2.8 eV. Fourier transform infrared spectroscopy analysis confirmed the presence of several functional groups on synthesized ZnO NPs. The total amount of synthesized ZnO NPs was obtained about 170 g for a synthesis reaction. By performing the antifungal activity assay, high doses of green synthesized ZnO NPs significantly inhibited growth of isolated Colletotrichum sp. (KUFC 021) on culture plates. Under greenhouse conditions, high doses of synthesized ZnO NPs also significantly reduced anthracnose symptoms on inoculated orchid leaves with the Colletotrichum sp. (KUFC 021).

Beneficial Effects of Zinc Oxide Nanoparticles on Plant Regeneration of Vetiver Grass (Vetiveria zizanioides L. Nash)

2017 ◽  
Vol 866 ◽  
pp. 25-28
Author(s):  
Chonnikarn Khunchuay ◽  
Kanokporn Sompornpailin
Keyword(s):  
Zinc Oxide ◽  
Plant Regeneration ◽  
Zinc Oxide Nanoparticles ◽  
Shoot Length ◽  
Optimal Concentration ◽  
Oxide Nanoparticles ◽  
Vetiver Grass ◽  
Vetiveria Zizanioides ◽  
Zno Nps ◽  
Positive Effects

Zinc is an essential micronutrient element for plant. Nowadays, Zinc oxide nanoparticles (ZnO NPs) are widely used and have been shown adverse effects to plant physiology. The optimal concentration of ZnO NPs added in plant regeneration medium (PRM) is studied in this experiment. Six-weeks old calli induced from the aseptic axillary buds of vetiver grass were cultured in PRM with various concentrations of ZnO NPs (5, 10, 20, 40 mgL-1). PRM containing 5, 10 mgL-1 ZnO NPs showed the highest percentage of plant regeneration frequency (95%) but did not have a statistic difference with other treatments. However, the averages of shoot length regenerated under both concentrations were 1.32-1.33 cm. These averages of shoot length had statistically different from averages of shoot length of the others. Moreover, calli regenerated under PRM containing 5 mgL-1 ZnO NPs condition showed the highest average number of shoots per callus (9.75 shoots) with a statistic difference. ZnO NPs at 20-40 mgL-1 in the PRM did not enhance the percentage of plant regeneration, but retarded calli development and shoot elongation. The optimal concentration of ZnO NPs in PRM has positive effects on regeneration and development of vetiver grass under tissue culture condition.

scholarly journals Biofabrication of zinc oxide nanoparticles, characterization and cytotoxicity against pediatric leukemia cell lines

2019 ◽  
Vol 9 (1) ◽  
pp. 56-62
Author(s):  
Zuliang Liu ◽  
Fang Chen ◽  
Zhongde Lu
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Leukemia Cell ◽  
Punica Granatum ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Pediatric Leukemia ◽  
Selected Area Electron Diffraction ◽  
Nanoparticles Characterization ◽  
Average Size

AbstractIn this work, we showed the synthesis of zinc oxide nanoparticles (ZnO NPs) using leaf extract of Punica granatum. Characterization results showed the synthesis of stable ZnO NPs with average size of 7 nm. Fourier transform infrared (FT-IR) and zeta potential results confirmed the capping of ZnO NPs with plant extract polyphenols. X-ray diffraction (XRD) and selected area electron diffraction (SAED) examinations showed the crystalline nature of prepared ZnO NPs. Further, the cytotoxic properties of the green synthesized ZnO NPs are examined against pediatric leukemia cells and the morphology of cells was studies under microscope. The results of cytotoxicity and microscopic images revealed the dose dependent cytotoxicity, indicating their prospects for the development of novel techniques and materials for pediatric leukemia cancer therapy.

Physico-Mechanical Properties of Starch-Based Nanocomposite Film Incorporated with Hydrothermally Synthesized Zinc Oxide Nanoparticles

2016 ◽  
Vol 872 ◽  
pp. 162-167 ◽  
Author(s):  
Yandi Andiyana ◽  
Nugraha Edhi Suyatma ◽  
Suliantari
Keyword(s):  
Mechanical Properties ◽  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Transmission Rate ◽  
Physical And Mechanical Properties ◽  
Zinc Nitrate ◽  
Absorption Capacity ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Average Size

Zinc oxide nanoparticles (ZnO-NPs) were successfully prepared by hydrothermal method at low temperatures (80°C) for 2 hours using zinc nitrate as starting materials. The average size of hydrothermally synthesized ZnO-NPs were comparable with commercial one as confirmed by Particle Size Analyzer (PSA). The incorporation of hydrothermally synthesized ZnO-NPs (0.5% and 1%, w/w) and glycerol as plasticizer (20%, w/w) into tapioca starch film significantly reduce water absorption capacity and water vapor transmission rate while increasing tensile strength and elongation at break of the composite film. These results suggest that hydrothermally synthesized ZnO-NPs have the potential as nanofiller to improve the physical and mechanical properties of biobased film.

Evaluation of the Effect of Nanoparticles Zinc Oxide/Camellia sinensis Complex on the Kidney of Rats Treated with Monosodium Glutamate: Antioxidant and Histological Approaches

2019 ◽  
Vol 20 (7) ◽  
pp. 542-550 ◽  
Author(s):  
Nahla S. El-Shenawy ◽  
Reham Z. Hamza ◽  
Fawziah A. Al-Salmi ◽  
Rasha A. Al-Eisa
Keyword(s):  
Zinc Oxide ◽  
Camellia Sinensis ◽  
Zinc Oxide Nanoparticles ◽  
Morphological Changes ◽  
Monosodium Glutamate ◽  
Oxide Nanoparticles ◽  
High Dose ◽  
Enzymatic Antioxidants ◽  
Zno Nps ◽  
Tea Extract

Background: Zinc oxide nanoparticles (ZnO NPs) are robustly used biomedicine. Moreover, no study has been conducted to explore the consequence of green synthesis of ZnO NPs with Camellia sinensis (green tea extract, GTE) on kidneys of rats treated with monosodium glutamate (MSG). Methods: Therefore, the objective of the research was designed to explore the possible defensive effect of GTE/ZnO NPs against MSG-induced renal stress investigated at redox and histopathological points. Results: The levels of urea and creatinine increased as the effect of a high dose of MSG, in addition, the myeloperoxidase and xanthine oxidase activates were elevated significantly with the high dose of MSG. The levels of non-enzymatic antioxidants (uric acid, glutathione, and thiol) were decreased sharply in MSG-treated rats as compared to the normal group. Conclusion: The data displayed that GTE/ZnO NPs reduced the effects of MSG significantly by reduction of the level peroxidation and enhancement intracellular antioxidant. These biochemical findings were supported by histopathology evaluation, which showed minor morphological changes in the kidneys of rats.

Zinc oxide nanoparticles help to enhance plant growth and alleviate abiotic stress: A review

2020 ◽  
Vol 21 ◽  
Author(s):  
Mohammad Faizan ◽  
Fangyuan Yu ◽  
Chen Chen ◽  
Ahmad Faraz ◽  
Shamsul Hayat
Keyword(s):  
Zinc Oxide ◽  
Plant Growth ◽  
Abiotic Stresses ◽  
Zinc Oxide Nanoparticles ◽  
Growth And Yield ◽  
Oxide Nanoparticles ◽  
Main Concern ◽  
Negative Effects ◽  
Zno Nps ◽  
Agricultural Yield

: Abiotic stresses arising from atmosphere change belie plant growth and yield, leading to food reduction. The cultivation of a large number of crops in the contaminated environment is a main concern of environmentalists in the present time. To get food safety, a highly developed nanotechnology is a useful tool for promoting food production and assuring sustainability. Nanotechnology helps to better production in agriculture by promoting the efficiency of inputs and reducing relevant losses. This review examines the research performed in the past to show how zinc oxide nanoparticles (ZnO-NPs) are influencing the negative effects of abiotic stresses. Application of ZnO-NPs is one of the most effectual options for considerable enhancement of agricultural yield globally under stressful conditions. ZnO-NPs can transform the agricultural and food industry with the help of several innovative tools in reversing oxidative stress symptoms induced by abiotic stresses. In addition, the effect of ZnO-NPs on physiological, biochemical, and antioxidative activities in various plants have also been examined properly. This review summarizes the current understanding and the future possibilities of plant-ZnO-NPs research.

scholarly journals Wound Healing Composite Materials of Bacterial Cellulose and Zinc Oxide Nanoparticles with Immobilized Betulin Diphosphate

Nanomaterials ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 713
Author(s):  
Nina Melnikova ◽  
Alexander Knyazev ◽  
Viktor Nikolskiy ◽  
Peter Peretyagin ◽  
Kseniia Belyaeva ◽  
...  
Keyword(s):  
Wound Healing ◽  
Zinc Oxide ◽  
Bacterial Cellulose ◽  
Zinc Oxide Nanoparticles ◽  
Wound Dressings ◽  
Oxide Nanoparticles ◽  
Burn Wound ◽  
Zno Nps ◽  
Wound Model ◽  
And Oxidative Stress

A design of new nanocomposites of bacterial cellulose (BC) and betulin diphosphate (BDP) pre-impregnated into the surface of zinc oxide nanoparticles (ZnO NPs) for the production of wound dressings is proposed. The sizes of crystalline BC and ZnO NPs (5–25%) corresponded to 5–6 nm and 10–18 nm, respectively (powder X-ray diffractometry (PXRD), Fourier-infrared (FTIR), ultraviolet (UV), atomic absorption (AAS) and photoluminescence (PL) spectroscopies). The biological activity of the wound dressings “BC-ZnO NPs-BDP” was investigated in rats using a burn wound model. Morpho-histological studies have shown that more intensive healing was observed during treatment with hydrophilic nanocomposites than the oleophilic standard (ZnO NPs-BDP oleogel; p < 0.001). Treatment by both hydrophilic and lipophilic agents led to increases in antioxidant enzyme activity (superoxide dismutase (SOD), catalase) in erythrocytes and decreases in the malondialdehyde (MDA) concentration by 7, 10 and 21 days (p < 0.001). The microcirculation index was restored on the 3rd day after burn under treatment with BC-ZnO NPs-BDP wound dressings. The results of effective wound healing with BC-ZnO NPs-BDP nanocomposites can be explained by the synergistic effect of all nanocomposite components, which regulate oxygenation and microcirculation, reducing hypoxia and oxidative stress in a burn wound.

scholarly journals Biosynthesis of Zinc Oxide Nanoparticles from Acacia nilotica (L.) Extract to Overcome Carbapenem-Resistant Klebsiella Pneumoniae

Molecules ◽  
2021 ◽  
Vol 26 (7) ◽  
pp. 1919
Author(s):  
Elsayim Rasha ◽  
AlOthman Monerah ◽  
Alkhulaifi Manal ◽  
Ali Rehab ◽  
Doud Mohammed ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Zinc Oxide ◽  
Klebsiella Pneumoniae ◽  
Zinc Oxide Nanoparticles ◽  
Acacia Nilotica ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
X Ray ◽  
Carbapenem Resistant

Recently, concerns have been raised globally about antimicrobial resistance, the prevalence of which has increased significantly. Carbapenem-resistant Klebsiella pneumoniae (KPC) is considered one of the most common resistant bacteria, which has spread to ICUs in Saudi Arabia. This study was established to investigate the antibacterial activity of biosynthesized zinc oxide nanoparticles (ZnO-NPs) against KPC in vitro and in vivo. In this study, we used the aqueous extract of Acacia nilotica (L.) fruits to mediate the synthesis of ZnO-NPs. The nanoparticles produced were characterized by UV-vis spectroscopy, zetasizer and zeta potential analyses, X-ray diffraction (XRD) spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and transmission electron microscopy (TEM). The antimicrobial activity of ZnO-NPs against KPC was determined via the well diffusion method, and determining minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), the results showed low MIC and MBC when compared with the MIC and MBC of Imipenem and Meropenem antibiotics. The results of in vitro analysis were supported by the results upon applying ZnO-NP ointment to promote wound closure of rats, which showed better wound healing than the results with imipenem ointment. The biosynthesized ZnO-NPs showed good potential for use against bacteria due to their small size, applicability, and low toxicity to human cells.

scholarly journals Scolicidal activity of biosynthesized zinc oxide nanoparticles by Mentha longifolia L. leaves against Echinococcus granulosus protoscolices

2021 ◽  
Author(s):  
Bushra H. Shnawa ◽  
Samir M. Hamad ◽  
Azeez A. Barzinjy ◽  
Payman A. Kareem ◽  
Mukhtar H. Ahmed
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Public Health Problem ◽  
Analytical Techniques ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Leaf Extracts ◽  
X Ray ◽  
Morphological Alterations ◽  
Mentha Longifolia

AbstractCystic echinococcosis is a public health problem in developing countries that practice sheep breeding extensively. In the current study, the protoscolicidal activity of biosynthesized zinc oxide nanoparticles (ZnO NPs) derived from Mentha longifolia L. leaf extracts was investigated. The resultant ZnO NPs were characterized by means of various analytical techniques, such as ultraviolet–visible (UV–Vis) spectrometry, Fourier transform infrared (FT-IR) spectrophotometry, X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX) analysis. The results showed that the ZnO NP had the highest scolicidal activity at 400 ppm concentration after 150 min of exposure time, showing 100% mortality rate. The treated protoscolices exhibited loss of viability with several morphological alterations. Hence, an easy and effective green synthesis of ZnO NPs, with efficient scolicidal potential, is reported in this study.

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