scholarly journals Manipulation of carotenoid metabolism stimulates biomass and stress tolerance in tomato

2021 ◽  
Author(s):  
José G Vallarino ◽  
Jianing Mi ◽  
Ivan Petřík ◽  
Ondrej Novak ◽  
Sandra Marcela Correa ◽  
...  
Keyword(s):  
Crop Production ◽  
Nutritional Value ◽  
Single Gene ◽  
Growth Conditions ◽  
Provitamin A ◽  
High Productivity ◽  
Yield Increase ◽  
Carotenoid Metabolism ◽  
Tomato Cultivars ◽  
New Generation

Improving yield, nutritional value and tolerance to abiotic stress are major targets of current breeding and biotechnological approaches that aim at increasing crop production and ensuring food security. Metabolic engineering of carotenoids, the precursor of Vitamin-A and plant hormones that regulate plant growth and response to adverse growth conditions, has been mainly focusing on provitamin A biofortification or the production of high-value carotenoids. Here, we show that the introduction of a single gene of the carotenoid biosynthetic pathway in different tomato cultivars simultaneously improved photosynthetic capacity and tolerance to various abiotic stresses (e.g., high light, salt, and drought), caused an up to 77% fruit yield increase and enhanced fruit's provitamin A content and shelf life. Our findings pave the way for developing a new generation of crops that combine high productivity and increased nutritional value with the capability to cope with climate change-related environmental challenges.

scholarly journals DETERMINATION OF THE GENETICALLY VALUABLE CORN SAMPLES AT THE CREATION OF THE SOURCE MATERIAL FOR THE CORN SELECTION

2016 ◽  
Vol 2 ◽  
pp. 44-50
Author(s):  
Larisa Chernobay
Keyword(s):  
Crop Production ◽  
Source Material ◽  
Artificial Infection ◽  
High Productivity ◽  
The Creation ◽  
New Generation ◽  
Selection Of ◽  
Selection Work

For selection of the lines of new generation were used the donors of tolerance to the disease exciters and introduced the modified methods of artificial infection and selection that allowed create the new source material of corn. There were received 302 lines of UKC (Ukraine, Kharkiv, Chernobay) tolerant to the bubble smut that are characterized with valuable agricultural signs. Among them were separated 21 lines with the high productivity and valuable agricultural signs: UKC 85-2, UKC 80-3, UKC 80-2, UKC 135-2, UKC 155-2, UKC 96, UKC 86, UKC 142, UKC 83, UKC 96-2, UKC 129-2, UKC 164,UKC 83-2, UKC 88-2, UKC 131, UKC 147, UKC 144-2, UKC 167,UKC 164-2, UKC 90-2, UKC 163-2; were created lines with the complex tolerance to the bubble smut and crop moth with the high productivity: UKC 144-2, UKC 167, UKC 164-2, UKC 90-2, UKC 163-2. The UKC lined were included into selection work in the laboratory of selection and seeding of the corn of the Institute of crop production of V. Y. Yuriev, NAAS of Ukraine.

scholarly journals Mutability of demographic noise in microbial range expansions

2021 ◽  
Author(s):  
QinQin Yu ◽  
Matti Gralka ◽  
Marie-Cécilia Duvernoy ◽  
Megan Sousa ◽  
Arbel Harpak ◽  
...  
Keyword(s):  
Gene Deletion ◽  
Genetic Manipulation ◽  
Single Gene ◽  
Population Level ◽  
Growth Conditions ◽  
Establishment Probability ◽  
In The Wild ◽  
Order Of Magnitude ◽  
Demographic Noise ◽  
Single Gene Deletion

AbstractDemographic noise, the change in the composition of a population due to random birth and death events, is an important driving force in evolution because it reduces the efficacy of natural selection. Demographic noise is typically thought to be set by the population size and the environment, but recent experiments with microbial range expansions have revealed substantial strain-level differences in demographic noise under the same growth conditions. Many genetic and phenotypic differences exist between strains; to what extent do single mutations change the strength of demographic noise? To investigate this question, we developed a high-throughput method for measuring demographic noise in colonies without the need for genetic manipulation. By applying this method to 191 randomly-selected single gene deletion strains from the E. coli Keio collection, we find that a typical single gene deletion mutation decreases demographic noise by 8% (maximal decrease: 81%). We find that the strength of demographic noise is an emergent trait at the population level that can be predicted by colony-level traits but not cell-level traits. The observed differences in demographic noise from single gene deletions can increase the establishment probability of beneficial mutations by almost an order of magnitude (compared to in the wild type). Our results show that single mutations can substantially alter adaptation through their effects on demographic noise and suggest that demographic noise can be an evolvable trait of a population.

Fodder legumes affecting sequential crop production and fertilizer N use efficiency

1985 ◽  
Vol 105 (1) ◽  
pp. 1-7 ◽  
Author(s):  
R. De ◽  
M. A. Salim Khan ◽  
M. S. Katti ◽  
V. Raja
Keyword(s):  
Pearl Millet ◽  
Crop Production ◽  
Cyamopsis Tetragonoloba ◽  
Fertilizer N ◽  
Available N ◽  
Yield Increase ◽  
Sudan Grass ◽  
Fodder Crops ◽  
Use Efficiency ◽  
Subsequent Crop

SUMMARYExperiments made with winter fodder crops, lucerne (Medicago sativa), berseem (Trifolium alexandrinum) and oats (Avena sativa) and summer fodder crops, cow pea (Vigna unguiculata), guar (Cyamopsis tetragonoloba), sunhemp (Crotolaria juncea) and pearl millet (Pennisetum americanum) showed that a sequential crop of Sudan grass yielded more after the legumes than after the cereal fodders, oats or pearl millet. The legume advantage was noted in the crop not given fertilizers but also when Sudan grass was given N fertilizer. The yield increase in Sudan grass grown after legumes was equivalent to 32–60 kg fertilizer N/ha applied to Sudan grass following pearl millet.After harvesting the legumes more available N and NO3-N was present in the soil and the apparent recovery of fertilizer N by a subsequent crop was increased by the legume.

scholarly journals Nutritional Characterization of a Traditional Cultivar of Tomato Grown Under Organic Conditions—cv. “Malacara”

2022 ◽  
Vol 8 ◽  
Author(s):  
María D. Raigón ◽  
María D. García-Martínez ◽  
Octavian P. Chiriac
Keyword(s):  
Organic Farming ◽  
Nutritional Value ◽  
Fruit Weight ◽  
Organic Production ◽  
Local Varieties ◽  
Yellow Color ◽  
Organic Farmers ◽  
Tomato Cultivars ◽  
Selection Of

The loss of genetic diversity due to the replacement of local tomato (Solanum lycopersicum L.) varieties by improved cultivars has been mitigated in many cases by the good work of organic farmers in maintaining local agricultural biodiversity. In parallel to these initiatives, in recent years, consumers have developed an increasing awareness of both food-related health, environmental issues, and food demand to recover the flavors of the past. In the case of tomatoes, these attributes (nutritional, organoleptic, social, and environmental) are closely related to organic production using local varieties. “Malacara” tomato is an example of a local variety. Coming from Sierra de Cádiz, it is a varietal type called “Cuelga” (“for hanging,” because the tomato trusses are hung from beams in the farmhouses). Cultivated and harvested in the open air during the summer months, these tomatoes are commercialized and consumed in the winter. Historically, this variety has enabled the fresh consumption of tomatoes during the winter, without the need to force cultivation. It is highly appreciated in the local cuisine and is the basis for sauces figuring in typical dishes. Its characteristic traits are small, pallid fruits, and long shelf life. The main objective of this work has been to typify two Malacara tomato cultivars (red and yellow color) grown under organic farming conditions, through the characterization of morphological, nutritional, and volatile parameters. The main differences are due to morphological parameters (fruit weight and color of the exocarp and endocarp). Other characteristics such as the content of ash, fiber, moisture, the concentration of iron, magnesium, and calcium, and content of lycopene are different between both cultivars. This study provides information on the nutritional and aromatic composition of two Malacara tomato cultivars, differentiated by their color and grown under organic farming conditions. The results add value to the native horticultural heritage and can aid in the selection of tomato varieties suitable for a sustainable production system and to produce tomatoes with high nutritional value and rich in aroma.

scholarly journals ПРОДУКТИВНІСТЬ ПРОБНИХ ГІБРИДІВ БУРЯКІВ ЦУКРОВИХ, СТВОРЕНИХ НА ОСНОВІ ПОЛІПШЕНОЇ СТЕРИЛЬНОЇ ФОРМИ ТА БАГАТОРОСТКОВИХ ЗАПИЛЮВАЧІВ РІЗНОГО ПОХОДЖЕННЯ

2012 ◽  
pp. 17-19
Author(s):  
Ф. М. Парій ◽  
М. П. Андрощук ◽  
С. Г. Труш
Keyword(s):  
Sugar Beet ◽  
Selection Process ◽  
High Productivity ◽  
Genetic Features ◽  
Important Direction ◽  
Productivity Indices ◽  
New Generation

Вивчення батьківських компонентів є важливимнапрямом досліджень. Використання в якості ма-теринської форми лінії ЧС 94 дозволяє створюва-ти гетерозисні гібриди з високим рівнем продук-тивності. Авторами вивчено вплив поліпшеної закомплексом селекційно-генетичних ознак стериль-ної лінії на прояв господарськи цінних ознак устворених гібридів цукрових буряків. Проведеновипробування отриманих гібридів та оціненопоказники їх продуктивності. Лінію ЧС 94рекомендовано залучати до селекційного процесу зформування високопродуктивних гібридів буряківцукрових нового покоління. Studying of parent components is an important direction for research. Using МС 94 line as a mother form makes it possible to create geterosis hybrids with high productivity. The authors investigated the influence of the improved in the complex of selection-genetic features sterile line as to their commercially valuablecharacteristics in the process of creating sugar beet hybrids. Testing of the received hybrids was conducted and their productivity indices were evaluated. MС 94 line is recommended to be involved in the selection process for the formation of highly productive sugar beet hybrids of new generation.

Analysis of Dislocation Behavior in Low Dislocation Density, PVT-Grown, Four-Inch Silicon Carbide Single Crystals

2010 ◽  
Vol 1246 ◽  
Author(s):  
Michael Dudley ◽  
Shayan Byrappa ◽  
Huanhuan Wang ◽  
Fangzhen Wu ◽  
Yu Zhang ◽  
...  
Keyword(s):  
Basal Plane ◽  
Growth Conditions ◽  
Formation Mechanisms ◽  
Stress Growth ◽  
Low Dislocation Density ◽  
Basal Plane Dislocation ◽  
Dislocation Behavior ◽  
New Generation ◽  
Insight Into

AbstractSynchrotron White Beam X-ray Topography studies are presented of dislocation behavior and interactions in a new generation of one hundred millimeter diameter, 4H-SiC wafers grown using Physical Vapor Transport (PVT) under specially designed low stress conditions. Such low stress growth conditions have, for example enabled reductions of basal plane dislocation (BPD) densities by two or three orders of magnitude compared to previous levels down to just a few hundred per square centimeter. This provides a unique opportunity to discern details of dislocation behavior which were previously precluded due to complications of image overlap at higher densities. Among the phenomena observed in these studies is the deflection of threading dislocations onto the basal plane producing various stacking fault configurations. Analysis of the contrast from these faults enables determination of their fault vectors which, in turn, provides insight into their possible formation mechanisms.

scholarly journals Plasmids for independently tunable, low-noise expression of two genes

2019 ◽  
Author(s):  
João P. N. Silva ◽  
Soraia Vidigal Lopes ◽  
Diogo J. Grilo ◽  
Zach Hensel
Keyword(s):  
Gene Expression ◽  
Single Molecule ◽  
Single Gene ◽  
Growth Conditions ◽  
Low Noise ◽  
Extrinsic Noise ◽  
Gene Expression Noise ◽  
Expression Noise ◽  
Compatible Plasmid ◽  
Noise Limit

AbstractSome microbiology experiments and biotechnology applications can be improved if it is possible to tune the expression of two different genes at the same time with cell-to-cell variation at or below the level of genes constitutively expressed from the chromosome (the “extrinsic noise limit”). This was recently achieved for a single gene by exploiting negative autoregulation by the tetracycline repressor (TetR) and bicistronic gene expression to reduce gene expression noise. We report new plasmids that use the same principles to achieve simultaneous, low-noise expression for two genes. The TetR system was moved to a compatible plasmid backbone, and a system based on the lac repressor (LacI) was found to also exhibit gene expression noise below the extrinsic noise limit. We characterize gene expression mean and noise across the range of induction levels for these plasmids, apply the LacI system to tune expression for single-molecule mRNA detection in two different growth conditions, and show that two plasmids can be co-transformed to independently tune expression of two different genes.

scholarly journals Perturbations of transcription and gene expression-associated processes alter distribution of cell size values in Saccharomyces cerevisiae

2018 ◽  
Author(s):  
Nairita Maitra ◽  
Jayamani Anandhakumar ◽  
Heidi M. Blank ◽  
Craig D. Kaplan ◽  
Michael Polymenis
Keyword(s):  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Rna Polymerase Ii ◽  
Cell Size ◽  
Gamma Distribution ◽  
Single Gene ◽  
Growth Conditions ◽  
Wild Type ◽  
Pol Ii ◽  
Diploid Cells

ABSTRACTThe question of what determines whether cells are big or small has been the focus of many studies because it is thought that such determinants underpin the coupling of cell growth with cell division. In contrast, what determines the overall pattern of how cell size is distributed within a population of wild type or mutant cells has received little attention. Knowing how cell size varies around a characteristic pattern could shed light on the processes that generate such a pattern and provide a criterion to identify its genetic basis. Here, we show that cell size values of wild type Saccharomyces cerevisiae cells fit a gamma distribution, in haploid and diploid cells, and under different growth conditions. To identify genes that influence this pattern, we analyzed the cell size distributions of all single-gene deletion strains in Saccharomyces cerevisiae. We found that yeast strains which deviate the most from the gamma distribution are enriched for those lacking gene products functioning in gene expression, especially those in transcription or transcription-linked processes. We also show that cell size is increased in mutants carrying altered activity substitutions in Rpo21p/Rpb1, the largest subunit of RNA polymerase II (Pol II). Lastly, the size distribution of cells carrying extreme altered activity Pol II substitutions deviated from the expected gamma distribution. Our results are consistent with the idea that genetic defects in widely acting transcription factors or Pol II itself compromise both cell size homeostasis and how the size of individual cells is distributed in a population.

scholarly journals Large Scale Demo Plot Trial Project of Potash Fertilization for Increased Yield and Profitability for Smallholder Soybean Fields in India

2019 ◽  
Vol 7 (2) ◽  
pp. 159-170
Author(s):  
Joachim B. Nachmansohn ◽  
Patricia Imas ◽  
Surinder K. Bansal
Keyword(s):  
Soil Fertility ◽  
Crop Production ◽  
Large Scale ◽  
Smallholder Farmers ◽  
Agricultural Practices ◽  
Crop Productivity ◽  
Fine Tuning ◽  
Soil Testing ◽  
Yield Increase ◽  
K Fertilization

Agriculture is the backbone of the Indian economy, in spite of concerned efforts towards industrialization in the last three decades. Therefore, the soil quality and fertility are the major factors in crop production. Declining soil fertility is one of the primary factors that directly affect crop productivity, and fertilizer-use is a key factor in order to keep soil fertility and productivity. A major factor in declining soil fertility is potassium (K) depletion, especially on smallholder farms where fertilization decisions are not based on regular soil testing. Most of the smallholder soybean producers do not have access and investment capacity to soil testing services. Therefore, there is a need to create K fertilizer recommendations based on empirically verified knowledge at India-specific scale. Such large-scale studies, in local filed conditions, are currently lacking. In order to bridge this gap, and generate proven set of directly applicable recommendations, a large-scale plot trial was launched; the Potash for Life (PFL) project. The study evaluated the K response in soybean when fertilizing with potash on K depleted soils in local variable field conditions. The aim was to (1) evaluate the effect and response consistency of K application on soybean yield, (2) to demonstrate to farmers the increased yield and profitability from K-inclusive fertilization regimes for this crop and give recommendations for transient yield increase, and (3) to raise the awareness among smallholder farmers about the importance of K fertilization. A comprehensive experiment was carried out in Madhya Pradesh (M.P.) and Maharashtra. The methodology was straight-forward; two identical plots side by side, with the only difference that one of them was fertilized with additional potash. The results showed a significant yield increase response from the potash application; the average yield increase was 244 kg ha-1 or 26 % in M.P., and 105 kg ha-1 or 36 % in Maharashtra. This entailed an average additional net profit of ₹ 6,681 INR ha-1 and ₹ 2,544 INR ha-1, in M.P. and Maharashtra respectively. It was concluded that the soil status of plant available K is significantly lower than the plant demand for soybean production in the two states, Consequently, K fertilization is necessary in order to improve agricultural practices and optimizing yields. Ultimately, following recommendations given in this study would allow farmers to generate additional profit, which could further allow them to invest in fine-tuning fertilizer practices through the means of soil testing.

scholarly journals Incorporating Water Harvesting into Plasticulture Production of Muskmelon

1999 ◽  
Vol 9 (1) ◽  
pp. 78-85 ◽  
Author(s):  
Frank J. Dainello ◽  
Larry Stein ◽  
Guy Fipps ◽  
Kenneth White
Keyword(s):  
Crop Production ◽  
Large Scale ◽  
Production Systems ◽  
Water Harvesting ◽  
Vegetable Crops ◽  
Plastic Mulch ◽  
Dry Land ◽  
Yield Increase ◽  
Harvesting Technique ◽  
Marketable Fruit

Competition for limited water supplies is increasing world wide. Especially hard hit are the irrigated crop production regions, such as the Lower Rio Grande Valley and the Winter Garden areas of south Texas. To develop production techniques for reducing supplemental water needs of vegetable crops, an ancient water harvesting technique called rainfall capture was adapted to contemporary, large scale irrigated muskmelon (Cucumis melo var. reticulatus L.) production systems. The rainfall capture system developed consisted of plastic mulched miniature water catchments located on raised seed beds. This system was compared with conventional dry land and irrigated melon production. Rainfall capture resulted in 108% average yield increase over the conventional dry land technique. When compared with conventional furrow irrigation, rainfall capture increased marketable muskmelon yield as much as 5355 lb/acre (6000 kg·ha-1). As anticipated,the drip irrigation/plastic mulch system exceeded rainfall capture in total and marketable fruit yield. The results of this study suggest that rainfall capture can reduce total supplemental water use in muskmelon production. The major benefit of the rainfall capture system is believed to be in its ability to eliminate or decrease irrigation water needed to fill the soil profile before planting.

Sign in / Sign up

Export Citation Format

Share Document