scholarly journals Controlled Administration of Dehydrochloromethyltestosterone in Humans: Urinary Excretion and Long-Term Detection of Metabolites for Anti-Doping Purpose

2021 ◽  
Author(s):  
Steffen Loke ◽  
Xavier de la Torre ◽  
Michele Iannone ◽  
Giuseppe La Piana ◽  
Nils Schloerer ◽  
...  
Keyword(s):  
Olympic Games ◽  
Ring Structure ◽  
Tandem Mass ◽  
Anabolic Androgenic Steroid ◽  
Healthy Male ◽  
Dose Administration ◽  
Interindividual Differences ◽  
Trial Monitoring ◽  
The 1960S

Dehydrochloromethyltestosterone (DHCMT) is an anabolic-androgenic steroid that was developed by Jenapharm in the 1960s and was marketed as Oral Turinabol. It is prohibited in sports at all times; nevertheless, there are several findings by anti-doping laboratories every year. New long-term metabolites have been proposed in 2011/12, which resulted in adverse analytical findings in retests of the Olympic games of 2008 and 2012. However, no controlled administration trial monitoring these long-term metabolites was reported until now. In this study, DHCMT (5 mg, p.o.) was administered to five healthy male volunteers and their urine samples were collected for a total of 60 days. The unconjugated and the glucuronidated fraction were analyzed separately by gas chromatography coupled to tandem mass spectrometry. The formation of the described long-term metabolites was verified, and their excretion monitored in detail. Due to interindividual differences there were several varieties in the excretion profiles among the volunteers. The metabolite M3, which has a fully reduced A-ring and modified D-ring structure, was identified by comparison with reference material as 4α- chloro-17β-hydroxymethyl-17α-methyl-18-nor-5α-androstan-13-en-3α-ol. It was found to be suitable as long-term marker for the intake of DHCMT in four of the volunteers. In one of the volunteers, it was detectable for 45 days after single oral dose administration. However, in two of the volunteers M5 (already published as long-term metabolite in the 1990s) showed longer detection windows. In one volunteer M3 was undetectable but another metabolite, M2, was found as the longest detectable metabolite. The last sample clearly identified as positive was collected between 9.9 and 44.9 days. Furthermore, the metabolite epiM4 (partially reduced A-ring and a modified D-ring structure which is epimerized in position 17 compared to M3) was identified in the urine of all volunteers with the help of chemically synthesized reference as 4-chloro-17α- hydroxymethyl-17β-methyl-18-nor-androsta-4,13-dien-3β-ol. It may serve as additional confirmatory metabolite. It is highly recommended to screen for all known metabolites in both fractions, glucuronidated and unconjugated, to improve identification of cheating athletes. This study also offers some deeper insights into the metabolism of DHCMT and of 17α- methyl steroids in general.

scholarly journals Doping practices in international weightlifting: analysis of sanctioned athletes/support personnel from 2008 to 2019 and retesting of samples from the 2008 and 2012 Olympic Games

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Alexander Kolliari-Turner ◽  
Brian Oliver ◽  
Giscard Lima ◽  
John P. Mills ◽  
Guan Wang ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
Regional Differences ◽  
Olympic Games ◽  
Anabolic Androgenic Steroid ◽  
Sample Storage ◽  
Support Personnel ◽  
Androgenic Steroid ◽  
In Doping ◽  
Targeted Testing

Abstract Background The pervasiveness of doping and findings of anti-doping corruption threaten weightlifting’s position at the 2024 Olympic Games. Analysing the practices of doping in weightlifters could identify patterns in doping that assist in future detection. Methods We analysed publicly available data on sanctioned athletes/support personnel from the International Weightlifting Federation between 2008 and 2019 and announced retrospective Anti-Doping Rule Violations (ADRVs) from the 2008 and 2012 Olympic Games. Results There were 565 sanctions between 2008 and 2019 of which 82% related to the detection of exogenous Anabolic Androgenic Steroid (AAS) metabolites and markers indicating endogenous AAS usage. The detection of exogenous AAS metabolites, markers of endogenous AAS usage and other substance metabolites varied by IWF Continental Federation (p ≤ 0.05) with Europe (74%, 11%, 15%) and Asia (70%, 15%, 15%) showing a higher detection of exogenous AAS compared to Pan America (37%, 30%, 33%) and Africa (50%, 17%, 33%). When looking at the 10 most detected substances, the nations with the highest number of sanctions (range 17–35) all had at least one overrepresented substance that accounted for 38–60% of all detected substances. The targeted re-analysis of samples from the 2008 and 2012 Olympic Games due to the discovery of long-term metabolites for exogenous AAS resulted in 61 weightlifters producing retrospective ADRVs. This includes 34 original medallists (9 gold, 10 silver and 15 bronze), the highest of any sport identified by Olympic Games sample re-testing. The exogenous AAS dehydrochloromethyltestosterone and stanozolol accounted for 83% of detected substances and were present in 95% of these samples. Conclusion Based on these findings of regional differences in doping practices, weightlifting would benefit from the targeted testing of certain regions and continuing investment in long-term sample storage as the sensitivity and specificity of detection continues to improve.

scholarly journals Doping Practices in International Weightlifting: Analysis of sanctioned athletes/support personnel from 2008-2019 and retesting of samples from the 2008 and 2012 Olympic Games

2020 ◽  
Author(s):  
Alexander Kolliari-Turner ◽  
Brian Oliver ◽  
Giscard Lima ◽  
John P. Mills ◽  
Guan Wang ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
Regional Differences ◽  
Olympic Games ◽  
Anabolic Androgenic Steroid ◽  
Sample Storage ◽  
Support Personnel ◽  
Androgenic Steroid ◽  
In Doping ◽  
Targeted Testing

Abstract Background: The pervasiveness of doping and findings of anti-doping corruption threaten weightlifting’s position at the 2024 Olympic Games. Analysing the practices of doping in weightlifters could identify patterns in doping that assist in future detection. Methods: We analysed publicly available data on sanctioned athletes/support personnel from the International Weightlifting Federation between 2008-2019 and announced retrospective Anti-Doping Rule Violations (ADRVs) from the 2008 and 2012 Olympic Games. Results: There were 565 sanctions between 2008-2019 of which 82% related to the detection of exogenous Anabolic Androgenic Steroid (AAS) metabolites and markers indicating endogenous AAS usage. The detection of exogenous AAS metabolites, markers of endogenous AAS usage and other substance metabolites varied by IWF Continental Federation (p ≤ 0.05) with Europe (74%, 11%, 15%) and Asia (70%, 15%, 15%) showing a higher detection of exogenous AAS compared to Pan America (37%, 30%, 33%) and Africa (50%, 17%, 33%). When looking at the 10 most detected substances, the nations with the highest number of sanctions (range 17-35) all had at least one overrepresented substance that accounted for 38-60% of all detected substances. The targeted re-analysis of samples from the 2008 and 2012 Olympic Games due to the discovery of long-term metabolites for exogenous AAS resulted in 61 weightlifters producing retrospective ADRVs. This includes 34 original medallists (9 Gold, 10 Silver and 15 Bronze), the highest of any sport identified by Olympic Games sample re-testing. The exogenous AAS Dehydrochloromethyltestosterone and Stanozolol accounted for 83% of detected substances and were present in 95% of these samples. Conclusion: Based on these findings of regional differences in doping practices, weightlifting would benefit from the targeted testing of certain regions and continuing investment in long-term sample storage as the sensitivity and specificity of detection continues to improve.

scholarly journals High Mountain Asian glacier response to climate revealed by multi-temporal satellite observations since the 1960s

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Atanu Bhattacharya ◽  
Tobias Bolch ◽  
Kriti Mukherjee ◽  
Owen King ◽  
Brian Menounos ◽  
...  
Keyword(s):  
Mass Loss ◽  
River Flow ◽  
Tien Shan ◽  
High Mountain ◽  
Glacier Mass Balance ◽  
Temperature And Precipitation ◽  
Multi Temporal ◽  
The 1960S ◽  
Northern Tien Shan

AbstractKnowledge about the long-term response of High Mountain Asian glaciers to climatic variations is paramount because of their important role in sustaining Asian river flow. Here, a satellite-based time series of glacier mass balance for seven climatically different regions across High Mountain Asia since the 1960s shows that glacier mass loss rates have persistently increased at most sites. Regional glacier mass budgets ranged from −0.40 ± 0.07 m w.e.a−1 in Central and Northern Tien Shan to −0.06 ± 0.07 m w.e.a−1 in Eastern Pamir, with considerable temporal and spatial variability. Highest rates of mass loss occurred in Central Himalaya and Northern Tien Shan after 2015 and even in regions where glaciers were previously in balance with climate, such as Eastern Pamir, mass losses prevailed in recent years. An increase in summer temperature explains the long-term trend in mass loss and now appears to drive mass loss even in regions formerly sensitive to both temperature and precipitation.

scholarly journals Parental support in sports development of Brazilian gymnasts participants in the Olympic Games (1980-2004)

2016 ◽  
Vol 30 (1) ◽  
pp. 109-118
Author(s):  
Laurita Marconi SCHIAVON ◽  
Daniela Bento SOARES
Keyword(s):  
Oral History ◽  
Parental Support ◽  
Development Process ◽  
Olympic Games ◽  
Sports Performance ◽  
Oral Testimony ◽  
The Subject ◽  
High Level ◽  
The Olympic Games

Abstract Sports development involves important aspects that collaborate towards the achievement of a high level sports performance. Parental support is one such fact to be considered in Long Term Athlete Development (LTAD), capable of benefiting or harming athletes if not adequately administered. This study registers and discusses the importance of parental support in female Artistic Gymnastics, from the perspective of Brazilian gymnasts who have participated in the Olympic Games. The method used was Oral History with the technique known as oral testimony. The participants of the study were the ten Brazilian gymnasts who represented Brazil in the Olympic Games from when the country first participated in this championship, in 1980, up to the best Brazilian classification in Athens (2004), totaling ten gymnasts (a sample comprising 100% or the research universe). Testimony analysis was conducted through crossanalysis. The study shows unanimity among the gymnasts in regards to the importance of parental support in the sports development process. In addition to reinforcing the results found in the literature, the testimonies provide details of the relationships between the gymnasts and their families for deeper reflections around the subject, a distinguishing feature of studies with oral testimonies.

Biological response genes after single dose administration of interferon β-1b to healthy male volunteers

2008 ◽  
Vol 199 (1-2) ◽  
pp. 115-125 ◽  
Author(s):  
Jan Hilpert ◽  
Johanna M. Beekman ◽  
Susanne Schwenke ◽  
Kristin Kowal ◽  
David Bauer ◽  
...  
Keyword(s):  
Single Dose ◽  
Biological Response ◽  
Healthy Male ◽  
Interferon Β ◽  
Dose Administration ◽  
Single Dose Administration ◽  
Healthy Male Volunteers ◽  
Male Volunteers

How the Desert got a Past: A History of Quaternary Research in Australia’s Deserts

2014 ◽  
Vol 25 (2) ◽  
pp. 172 ◽  
Author(s):  
Mike Smith
Keyword(s):  
Climate Changes ◽  
Conceptual Space ◽  
The Past ◽  
History Of ◽  
The 1960S

This paper examines how the past of desert landscapes has been interpreted since European explorers and scientists first encountered them. It charts the research that created the conceptual space within which archaeologists and Quaternarists now work. Studies from the 1840s–1960s created the notion of a ‘Great Australian Arid Period'. The 1960s studies of Lake Mungo and the Willandra Lakes by Jim Bowler revealed the cyclical nature of palaeolakes, that changed with climate changes in the Pleistocene, and the complexity of desert pasts. SLEADS and other researchers in the 1980s used thermoluminescence techniques that showed further complexities in desert lands beyond the Willandra particularly through new studies in the Strzelecki and Simpson Dunefields, Lake Eyre, Lake Woods and Lake Gregory. Australian deserts are varied and have very different histories. Far from ‘timeless lands', they have carried detailed information about long-term climate changes on continental scales.

Islamophobia

2019 ◽  
pp. 244-271
Author(s):  
Martin Pugh
Keyword(s):  
The United States ◽  
Islamic State ◽  
Islamic Fundamentalism ◽  
The West ◽  
Small Minority ◽  
Large Numbers ◽  
Proximate Causes ◽  
To Come ◽  
The 1960S

This chapter discusses how, misled by Islamophobic propaganda, Britain and America were unable to come to terms with what they called ‘Islamism’. The origins of what is variously known as Islamism, Islamic fundamentalism, and radical Islamism lie in the 1960s, in the ideas of a handful of Muslims in Pakistan, Egypt, and Iran who believed that Muslims had been led astray from their religion by nationalist movements. Although some Muslims were critical of Western morality and politics, Islamism was not primarily anti-Western: it was essentially a reaction against what were widely seen as the corrupt, authoritarian, and secular regimes that controlled much of the Muslim world. The aim was to evict them, return to a purer form of Islam and re-create an Islamic state. In view of the exaggerated reputation it enjoys in the West, it is worth remembering that this movement has largely been a failure. Yet while fundamentalism appeals to only a small minority, it is also the case that large numbers of Muslims have become aggrieved by the policies of the Western powers. The explanation for this can be found in long-term frustration with the consistently pro-Israeli policy of Britain and the United States over Palestine, in addition to the proximate causes in the shape of two Afghan wars, the genocide in Bosnia, the Rushdie affair, and the first Gulf War in 1990, which made many Muslims see themselves as the victims of Western aggression and interventionism.

scholarly journals Effects of long-term repeated chemical disinfection treatment on the surface hardness self-polymerizing reline resins

2013 ◽  
Vol 1 (1) ◽  
pp. 55
Author(s):  
Luciana de Rezende Pinto ◽  
Paulo Maurício Batista Silva ◽  
Vinicius Carvalho Porto
Keyword(s):  
Surface Hardness ◽  
Knoop Hardness ◽  
Control Group ◽  
Tukey Test ◽  
Acrylic Resins ◽  
Biofilm Removal ◽  
Chemical Solutions ◽  
1960S And 1970S ◽  
The 1960S

Denture hygiene techniques and procedures were developed in the 1960s and 1970s and most studies indicate the importance of mechanical biofilm removal by denture brushing associated with disinfection with chemical solutions. Studies in the literature show many chemical procedures that may be used for denture biofilm control. When the immersion procedure is used, the disinfectant should be selected with regard to its effectiveness in inactivating microorganisms without any adverse effects on the denture materials. PURPOSE: This study investigated the hardness of three self-polymerizing reline resins after long-term repeated chemical disinfections. MATERIAL AND METHODS: Sixty round specimens (30 x 6mm) were made from each material: Jet, Kooliner and Tokuyama Rebase II Fast, and divided in 6 groups (n=10). The control group was stored in water and the others were disinfected with 1%, 2%, 5.25% sodium hypochlorite, 2% glutaraldehyde, and 4% chlorhexidine gluconate, respectively. The specimens were tested for knoop hardness (KHN) before disinfection and after 30, 90 and 180 disinfection cycles. Data were analyzed by analysis of variance followed by the Tukey test at 5%. RESULTS: The hardness of Jet resin varied from 18.74 ± 0.47 to 13.75 ± 0.95 KHN, Kooliner varied from 14.09 ± 1.63 to 7.52 ± 0.88 KHN, and Tokuyama Rebase II Fast from 12.57 ± 0.94 to 8.28 ± 0.39 KHN. Statistically significant decrease in hardness of the three reline acrylic resins was observed early after the first 30 disinfection cycles. CONCLUSION: The hardness of the tested materials decreased after immersion in water and after long-term repeated chemical disinfections.

scholarly journals Occurrence and spatial-temporal distribution of herbicide residues in the Ipojuca River sub-basin, Pernambuco, Brazil

2016 ◽  
Vol 20 (12) ◽  
pp. 1124-1128 ◽  
Author(s):  
Adson da S. G. Ferreira ◽  
Henrique C. M. P. da Silva ◽  
Hélio O. S. Rodrigues ◽  
Marileide Silva ◽  
Eden C. de Albuquerque Junior
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Temporal Distribution ◽  
Tandem Mass ◽  
Herbicide Residues ◽  
Ecological Changes ◽  
Biological Potential ◽  
Intensive Use

ABSTRACT The intensive use of pesticides to control pests in agriculture has exposed the environment and humans to a variety of risks. Among the crops with higher consumption of these compounds there is the sugarcane, developed in regions bordered by large watersheds. In this work, the occurrence of pesticides in the water of Ipojuca River was investigated in a 50 km range of its eastern portion, in a region noted for intense agroindustrial activity, especially by sugarcane cultivation, in the state of Pernambuco. Among fungicides, herbicides, insecticides and carbamates, 238 pesticides were investigated in the Ipojuca River using the technique of liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). The study, conducted in the months of May, June, October and November 2012, detected the presence of Diuron and Ametryn herbicide residues in 100% of the water samples at concentrations from 0.01 to 1.4 μg L-1. The detection of these herbicides, even at residual concentrations, can lead to perceptible ecological changes in the long term, such as the reduction of the biological potential of animal and plant species.

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