Regulation of drought tolerance in Arabidopsis involves PLATZ4-mediated transcriptional suppression of PIP2;8

PLATZ transcription factors play important roles in plant growth, development, biotic and abiotic stress responses. However, how PLATZ regulates plant drought tolerance and ABA sensitivity remains largely unknown. Here, we show that PLATZ4 increases drought tolerance and ABA sensitivity in Arabidopsis thaliana by suppressing the expression of PIP2;8, while upregulating expression of ABI3, ABI4 and ABI5. PLATZ4 directly binds A/T-rich sequences within the PIP2;8 promoter. Consistent with this, PIP2;8 acts epistatically to PLATZ4. Furthermore, the aquaporin activity of PIP2;8 was confirmed in Xenopus laevis oocytes in response to osmotic stress. Analysis of water loss of seedlings overexpressing PIP2;8 or lacking PIP2;8 function indicated that PIP2;8-mediated water flow is particularly active in response to drought stress in planta. In platz4 mutant and PLATZ4-overexpressing plants, water loss and stomatal closure changed oppositely to those in pip2;8 mutants and PIP2;8-overexpressing plants, respectively. In addition, the interaction between PLATZ4 and AITR6 was confirmed by several assays, and the binding of PIP2;8 promoter by PLATZ4 was strengthened by an interaction with AITR6. Collectively, our findings reveal that PLATZ4 interacts with AITR6 to increase ABA sensitivity and drought tolerance by upregulating expression of ABI3, ABI4 and ABI5 while inhibiting the expression of PIP2;8 and associated genes.

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Rice lectin protein Osr40c1 imparts drought tolerance by modulating OsSAM2, OsSAP8 and chromatin-associated proteins

10.1101/2020.04.19.049288 ◽

2020 ◽

Author(s):

Salman Sahid ◽

Chandan Roy ◽

Soumitra Paul ◽

Riddhi Datta

Keyword(s):

Drought Stress ◽

Drought Tolerance ◽

Stress Responses ◽

Ectopic Expression ◽

In Planta ◽

Biotic And Abiotic Stress ◽

Polyamine Biosynthesis ◽

Protein Partners ◽

Associated Proteins ◽

Lectin Protein

AbstractLectin proteins play an important role in biotic and abiotic stress responses in plants. Although the rice lectin protein, Osr40c1, has been reported to be regulated by drought stress, the mechanism of its drought tolerance activity has not been studied so far. In this study, it has been depicted that expression of Osr40c1 gene correlates with the drought tolerance potential of various rice cultivars. Transgenic rice plants overexpressing Osr40c1 were significantly more tolerant to drought stress over the wild-type plants. Furthermore, ectopic expression of the Osr40c1 gene in tobacco yielded a similar result. Interestingly, the protein displayed a nucleo-cytoplasmic localization and was found to interact with a number of drought-responsive proteins like OsSAM2, OsSAP8, OsMNB1B, and OsH4. Fascinatingly, silencing of each of these protein partners led to drought susceptibility in the otherwise tolerant Osr40c1 expressing transgenic tobacco lines indicating that these partners were crucial for the Osr40c1-mediated drought tolerance in planta. Together, the present investigation delineated the novel role of Osr40c1 protein in imparting drought tolerance by regulating the chromatin proteins, OsMNB1B and OsH4, which presumably enables OsSAP8 to induce downstream gene expression. In addition, its interaction with OsSAM2 might induce polyamine biosynthesis thus further improving drought tolerance in plants.HighlightsA rice lectin protein, Osr40c1, plays a crucial role in imparting drought stress tolerance in plants by modulating OsSAM2 as well as the transcriptional regulators OsSAP8, OsMNB1B and OsH4.

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Rice lectin protein r40c1 imparts drought tolerance by modulating S-adenosylmethionine synthase 2, stress-associated protein 8 and chromatin-associated proteins

Journal of Experimental Botany ◽

10.1093/jxb/eraa400 ◽

2020 ◽

Vol 71 (22) ◽

pp. 7331-7346 ◽

Cited By ~ 2

Author(s):

Salman Sahid ◽

Chandan Roy ◽

Soumitra Paul ◽

Riddhi Datta

Keyword(s):

Drought Stress ◽

Drought Tolerance ◽

Stress Responses ◽

Ectopic Expression ◽

In Planta ◽

Biotic And Abiotic Stress ◽

Protein Partners ◽

Stress Associated Protein ◽

Associated Proteins ◽

Lectin Protein

Abstract Lectin proteins play an important role in biotic and abiotic stress responses in plants. Although the rice lectin protein Osr40c1 has been reported to be regulated by drought stress, the mechanism of its drought tolerance activity has not been studied so far. In this study, it is shown that expression of the Osr40c1 gene correlates with the drought tolerance potential of various rice cultivars. Transgenic rice plants overexpressing Osr40c1 were significantly more tolerant to drought stress than the wild-type plants. Furthermore, ectopic expression of the Osr40c1 gene in tobacco yielded a similar result. Interestingly, the protein displayed a nucleo-cytoplasmic localization and was found to interact with a number of drought-responsive proteins such as S-adenosylmethionine synthase 2 (OsSAM2), stress-associated protein 8 (OsSAP8), DNA-binding protein MNB1B (OsMNB1B), and histone 4 (OsH4). Silencing of each of these protein partners led to drought sensitivity in otherwise tolerant Osr40c1-expressing transgenic tobacco lines indicating that these partners were crucial for the Osr40c1-mediated drought tolerance in planta. Moreover, the association of Osr40c1 with these partners occurred specifically under drought stress forming a multi-protein complex. Together, our findings delineate a novel role of Osr40c1 in imparting drought tolerance by regulating OsMNB1B, OsSAM2, and OsH4 proteins, which presumably enables OsSAP8 to induce downstream gene expression.

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Physiological and morphological responses to water stress in Aegilops biuncialis and Triticum aestivum genotypes with differing tolerance to drought

Functional Plant Biology ◽

10.1071/fp03143 ◽

2004 ◽

Vol 31 (12) ◽

pp. 1149 ◽

Cited By ~ 66

Author(s):

István Molnár ◽

László Gáspár ◽

Éva Sárvári ◽

Sándor Dulai ◽

Borbála Hoffmann ◽

...

Keyword(s):

Growth Rate ◽

Triticum Aestivum ◽

Water Stress ◽

Drought Tolerance ◽

Osmotic Stress ◽

Biomass Production ◽

Water Loss ◽

Stomatal Closure ◽

Co2 Assimilation ◽

Wheat Genotypes

The physiological and morphological responses to water stress induced by polyethylene glycol (PEG) or by withholding water were investigated in Aegilops biuncialis Vis. genotypes differing in the annual rainfall of their habitat (1050, 550 and 225 mm year–1) and in Triticum aestivum L. wheat genotypes differing in drought tolerance. A decrease in the osmotic pressure of the nutrient solution from –0.027 to –1.8 MPa resulted in significant water loss, a low degree of stomatal closure and a decrease in the intercellular CO2 concentration (Ci) in Aegilops genotypes originating from dry habitats, while in wheat genotypes high osmotic stress increased stomatal closure, resulting in a low level of water loss and high Ci. Nevertheless, under saturating light at normal atmospheric CO2 levels, the rate of CO2 assimilation was higher for the Aegilops accessions, under high osmotic stress, than for the wheat genotypes. Moreover, in the wheat genotypes CO2 assimilation exhibited less or no O2 sensitivity. These physiological responses were manifested in changes in the growth rate and biomass production, since Aegilops (Ae550, Ae225) genotypes retained a higher growth rate (especially in the roots), biomass production and yield formation after drought stress than wheat. These results indicate that Aegilops genotypes, originating from a dry habitat have better drought tolerance than wheat, making them good candidates for improving the drought tolerance of wheat through intergeneric crossing.

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Comprehensive transcriptome resource for response to phytohormone-induced signaling in Capsicum annuum L.

BMC Research Notes ◽

10.1186/s13104-020-05281-1 ◽

2020 ◽

Vol 13 (1) ◽

Author(s):

Junesung Lee ◽

Jae-Young Nam ◽

Hakgi Jang ◽

Nayoung Kim ◽

Yong-Min Kim ◽

...

Keyword(s):

Rna Sequencing ◽

Stress Responses ◽

Molecular Networks ◽

Response To Treatment ◽

Pepper Plant ◽

Transcriptome Data ◽

Biotic And Abiotic Stress ◽

Data Description ◽

Growth Development ◽

Transcriptome Resource

Abstract Objectives Phytohormones are small signaling molecules with crucial roles in plant growth, development, and environmental adaptation to biotic and abiotic stress responses. Despite several previously published molecular studies focused on plant hormones, our understanding of the transcriptome induced by phytohormones remains unclear, especially in major crops. Here, we aimed to provide transcriptome dataset using RNA sequencing for phytohormone-induced signaling in plant. Data description We used high-throughput RNA sequencing profiling to investigate the pepper plant response to treatment with four major phytohormones (salicylic acid, jasmonic acid, ethylene, and abscisic acid). This dataset yielded 78 samples containing three biological replicates per six different time points for each treatment and the control, constituting 187.8 Gb of transcriptome data (2.4 Gb of each sample). This comprehensive parallel transcriptome data provides valuable information for understanding the relationships and molecular networks that regulate the expression of phytohormone-related genes involved in plant developments and environmental stress adaptation.

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A tubby-like protein CsTLP8 acts in the ABA signaling pathway and negatively regulates osmotic stresses tolerance during seed germination

BMC Plant Biology ◽

10.1186/s12870-021-03126-y ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Shuangtao Li ◽

Zhirong Wang ◽

Fei Wang ◽

Hongmei Lv ◽

Meng Cao ◽

...

Keyword(s):

Seed Germination ◽

Osmotic Stress ◽

Stress Responses ◽

Transcriptional Activation ◽

Stomatal Closure ◽

Negative Regulator ◽

Biotic And Abiotic Stress ◽

Growth Activity ◽

A Subunit ◽

Antioxidant Enzymes Activities

Abstract Background TLPs (Tubby-like proteins) are widespread in eukaryotes and highly conserved in plants and animals. TLP is involved in many biological processes, such as growth, development, biotic and abiotic stress responses, while the underlying molecular mechanism remains largely unknown. In this paper we characterized the biological function of cucumber (Cucumis sativus L.) Tubby-like protein 8 (CsTLP8) in Arabidopsis. Results In cucumber, the expression of the tubby-like protein CsTLP8 was induced by NaCl treatment, but reduced by PEG (Polyethylene Glycol) and ABA (Abscisic Acid) treatment. Subcellular localization and transcriptional activation activity analysis revealed that CsTLP8 possessed two characteristics of classical transcription factors: nuclear localization and trans-activation activity. Yeast two-hybrid assay revealed interactions of CsTLP8 with CsSKP1a and CsSKP1c, suggesting that CsTLP8 might function as a subunit of E3 ubiquitin ligase. The growth activity of yeast with ectopically expressed CsTLP8 was lower than the control under NaCl and mannitol treatments. Under osmotic and salt stresses, overexpression of CsTLP8 inhibited seed germination and the growth of Arabidopsis seedlings, increased the content of MDA (Malondialdehyde), and decreased the activities of SOD (Superoxide Dismutase), POD (Peroxidase) and CAT (Catalase) in Arabidopsis seedlings. Overexpression of CsTLP8 also increased the sensitivity to ABA during seed germination and ABA-mediated stomatal closure. Conclusion Under osmotic stress, CsTLP8 might inhibit seed germination and seedling growth by affecting antioxidant enzymes activities. CsTLP8 acts as a negative regulator in osmotic stress and its effects may be related to ABA.

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Shoot Characterization of Isoprene and Ocimene-Emitting Transgenic Arabidopsis Plants under Contrasting Environmental Conditions

Plants ◽

10.3390/plants9040477 ◽

2020 ◽

Vol 9 (4) ◽

pp. 477 ◽

Cited By ~ 2

Author(s):

Michele Faralli ◽

Mingai Li ◽

Claudio Varotto

Keyword(s):

Water Stress ◽

Stress Tolerance ◽

Stress Responses ◽

Water Conservation ◽

Transgenic Arabidopsis ◽

Stomatal Closure ◽

Crop Improvement ◽

Dry Weight ◽

Co2 Assimilation ◽

Biotic And Abiotic Stress

Isoprenoids are among the most abundant biogenic volatile compounds (VOCs) emitted by plants, and mediate both biotic and abiotic stress responses. Here, we provide for the first time a comparative analysis of transgenic Arabidopsis lines constitutively emitting isoprene and ocimene. Transgenic lines and Columbia-0 (Col-0) Arabidopsis were characterized under optimal, water stress, and heat stress conditions. Under optimal conditions, the projected leaf area (PLA), relative growth rate, and final dry weight were generally higher in transgenics than Col-0. These traits were associated to a larger photosynthetic capacity and CO2 assimilation rate at saturating light. Isoprene and ocimene emitters displayed a moderately higher stress tolerance than Col-0, showing higher PLA and gas-exchange traits throughout the experiments. Contrasting behaviors were recorded for the two overexpressors under water stress, with isoprene emitters showing earlier stomatal closure (conservative behavior) than ocimene emitters (non-conservative behavior), which might suggest different induced strategies for water conservation and stress adaptation. Our work indicates that (i) isoprene and ocimene emitters resulted in enhanced PLA and biomass under optimal and control conditions and that (ii) a moderate stress tolerance is induced when isoprene and ocimene are constitutively emitted in Arabidopsis, thus providing evidence of their role as a potential preferable trait for crop improvement.

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Heterologous Expression of Arabidopsis rty Enhances Drought Tolerance in Strawberry (Fragaria × ananassa Duch.)

10.21203/rs.3.rs-34565/v1 ◽

2020 ◽

Cited By ~ 3

Author(s):

Maofu Li ◽

Yuan Yang ◽

Ali Raza ◽

Shanshan Yin ◽

Hua Wang ◽

...

Keyword(s):

Transgenic Plants ◽

Drought Tolerance ◽

Heterologous Expression ◽

Water Use ◽

Water Loss ◽

Loss Rate ◽

Stomatal Closure ◽

Fragaria Ananassa ◽

Water Loss Rate ◽

Use Efficiency

Abstract Background Strawberry ( Fragaria × ananassa Duch.) is an important fruit crop worldwide. It was particularly sensitive to drought stress because of their fibrous and shallow root systems. Mutation of Arabidopsis thaliana ROOTY ( RTY ) results in increased endogenous auxin levels and roots and shoot growth, but the effects of this gene in strawberry remain unclear. Results Here, we heterologously expressed Arabidopsis rty in strawberry plants and examined the effects of rty expression on the hormonal and physiological properties of the plants. Heterologous expression of rty induced IAA accumulation and increased the production of adventitious roots as well as trichomes on the abaxial leaf surface of the transgenic plants. Furthermore, the transgenic strawberry plants had increased ABA accumulation and stomatal closure. The transgenic strawberry plants exhibited enhanced water use efficiency and a reduced water loss rate. Additionally, peroxidase and catalase activities were significantly higher in the transgenic plants than in the untransformed controls, and the transgenic plants were more drought tolerant than the wild-type plants. Our results uncover a transgenic approaches can be used to overcome the inherent trade-off between plant growth and drought tolerance by enhancing water use efficiency and reducing water loss rate under water shortage conditions. Conclusions In this study, the rty gene improves hormone-mediated drought tolerance in transgenic strawberry. We demonstrated that the heterologous expression of rty in strawberry improved drought tolerance by promoting auxin and ABA accumulation. These phytohormones together brought about various physiological changes that improved drought tolerance via increased root production, trichome density, and stomatal closure. This study provides the basis for future genetic modifications of strawberry to improve drought tolerance.

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Arabidopsis SH3P2 is an ubiquitin-binding protein that functions together with ESCRT-I and the deubiquitylating enzyme AMSH3

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1710866114 ◽

2017 ◽

Vol 114 (34) ◽

pp. E7197-E7204 ◽

Cited By ~ 21

Author(s):

Marie-Kristin Nagel ◽

Kamila Kalinowska ◽

Karin Vogel ◽

Gregory D. Reynolds ◽

Zhixiang Wu ◽

...

Keyword(s):

Plasma Membrane ◽

Membrane Proteins ◽

Stress Responses ◽

Binding Protein ◽

In Planta ◽

Biotic And Abiotic Stress ◽

Plasma Membrane Proteins ◽

Endosomal Sorting ◽

Escrt Machinery ◽

Ubiquitin Binding

Clathrin-mediated endocytosis of plasma membrane proteins is an essential regulatory process that controls plasma membrane protein abundance and is therefore important for many signaling pathways, such as hormone signaling and biotic and abiotic stress responses. On endosomal sorting, plasma membrane proteins maybe recycled or targeted for vacuolar degradation, which is dependent on ubiquitin modification of the cargos and is driven by the endosomal sorting complexes required for transport (ESCRTs). Components of the ESCRT machinery are highly conserved among eukaryotes, but homologs of ESCRT-0 that are responsible for recognition and concentration of ubiquitylated proteins are absent in plants. Recently several ubiquitin-binding proteins have been identified that serve in place of ESCRT-0; however, their function in ubiquitin recognition and endosomal trafficking is not well understood yet. In this study, we identified Src homology-3 (SH3) domain-containing protein 2 (SH3P2) as a ubiquitin- and ESCRT-I–binding protein that functions in intracellular trafficking. SH3P2 colocalized with clathrin light chain-labeled punctate structures and interacted with clathrin heavy chain in planta, indicating a role for SH3P2 in clathrin-mediated endocytosis. Furthermore, SH3P2 cofractionates with clathrin-coated vesicles (CCVs), suggesting that it associates with CCVs in planta. Mutants of SH3P2 and VPS23 genetically interact, suggesting that they could function in the same pathway. Based on these results, we suggest a role of SH3P2 as an ubiquitin-binding protein that binds and transfers ubiquitylated proteins to the ESCRT machinery.

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Sensitive to Proton Rhizotoxicity1 Regulates Salt and Drought Tolerance of Arabidopsis thaliana through Transcriptional Regulation of CIPK23

Plant and Cell Physiology ◽

10.1093/pcp/pcz120 ◽

2019 ◽

Vol 60 (9) ◽

pp. 2113-2126 ◽

Cited By ~ 5

Author(s):

Ayan Sadhukhan ◽

Takuo Enomoto ◽

Yuriko Kobayashi ◽

Toshihiro Watanabe ◽

Satoshi Iuchi ◽

...

Keyword(s):

Drought Tolerance ◽

Root Growth ◽

Stomatal Closure ◽

Nacl Stress ◽

Insertion Mutant ◽

In Planta ◽

Short Root ◽

Drought Tolerant ◽

Dna Insertion ◽

Insertion Mutants

Abstract The transcription factor sensitive to proton rhizotoxicity 1 (STOP1) regulates multiple stress tolerances. In this study, we confirmed its involvement in NaCl and drought tolerance. The root growth of the T-DNA insertion mutant of STOP1 (stop1) was sensitive to NaCl-containing solidified MS media. Transcriptome analysis of stop1 under NaCl stress revealed that STOP1 regulates several genes related to salt tolerance, including CIPK23. Among all available homozygous T-DNA insertion mutants of the genes suppressed in stop1, only cipk23 showed a NaCl-sensitive root growth phenotype comparable to stop1. The CIPK23 promoter had a functional STOP1-binding site, suggesting a strong CIPK23 suppression led to NaCl sensitivity of stop1. This possibility was supported by in planta complementation of CIPK23 in the stop1 background, which rescued the short root phenotype under NaCl. Both stop1 and cipk23 exhibited a drought tolerant phenotype and increased abscisic acid-regulated stomatal closure, while the complementation of CIPK23 in stop1 reversed these traits. Our findings uncover additional pleiotropic roles of STOP1 mediated by CIPK23, which regulates various ion transporters including those regulating K+-homeostasis, which may induce a trade-off between drought tolerance and other traits.

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ZmASR3 from the Maize ASR Gene Family Positively Regulates Drought Tolerance in Transgenic Arabidopsis

International Journal of Molecular Sciences ◽

10.3390/ijms20092278 ◽

2019 ◽

Vol 20 (9) ◽

pp. 2278 ◽

Cited By ~ 5

Author(s):

Yani Liang ◽

Yingli Jiang ◽

Ming Du ◽

Baoyan Li ◽

Long Chen ◽

...

Keyword(s):

Drought Stress ◽

Drought Tolerance ◽

Stress Responses ◽

Stomatal Closure ◽

Molecular Features ◽

Ros Accumulation ◽

Drought Conditions ◽

Relative Water ◽

Transgenic Lines ◽

Related Stress

Abscisic acid (ABA)-, stress-, and ripening-induced (ASR) proteins are reported to be involved in drought stress responses. However, the function of maize ASR genes in enhancing drought tolerance is not known. Here, nine maize ASR members were cloned, and the molecular features of these genes were analyzed. Phenotype results of overexpression of maize ZmASR3 gene in Arabidopsis showed lower malondialdehyde (MDA) levels and higher relative water content (RWC) and proline content than the wild type under drought conditions, demonstrating that ZmASR3 can improve drought tolerance. Further experiments showed that ZmASR3-overexpressing transgenic lines displayed increased stomatal closure and reduced reactive oxygen species (ROS) accumulation by increasing the enzyme activities of superoxide dismutase (SOD) and catalase (CAT) under drought conditions. Moreover, overexpression of ZmASR3 in Arabidopsis increased ABA content and reduced sensitivity to exogenous ABA in both the germination and post-germination stages. In addition, the ROS-related, stress-responsive, and ABA-dependent pathway genes were activated in transgenic lines under drought stress. Taken together, these results suggest that ZmASR3 acts as a positive regulator of drought tolerance in plants.

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