scholarly journals Immunological female role tested on artificial plugs in three scorpion species

2018 ◽  
Author(s):  
Mariela A. Oviedo-Diego ◽  
Camilo I. Mattoni ◽  
Alfredo V. Peretti

AbstractWithin arachnids, genital plugs are morphologically diverse, and they can be formed by male, female or be a contribution of both sexes. Although several species of scorpions with genital plugs are known, the physiological effects on the female after being plugged have not been well studied yet. This work compares three scorpion species, two with genital plugs and one without. We first describe the genital plugs morphology of twoUrophoniusspecies. Second, through the placement of artificial genital plugs in the female genital atrium, we tested 1) whether there are interspecific differences in the immune encapsulation response on the artificial genital plug, 2) if there are an effect in the hemocyte load in the hemolymph, and 3) if individual’s immunological parameters and body weight are correlated. Additionally, we describe and quantify the hemocytes in these species. In both species ofUrophonius, genital plugs were found covering the female genital aperture and blocking the genital atrium. The plugs consist of three zones that are distinct in morphology and coloration. We found different patterns of encapsulation and melanization on the artificial plug according to the species, with a greater and more specific response in females of plug producing species. Also, these species showed a decrease in the hemocyte load one month after the placement of the artificial plug, possibly due to the recirculation of the hemocytes into the genital area. In addition, correlations were found between the body weight and the immunological parameters, as well as between different immunological parameters. Our results suggest that females contribute to the formation of genital plugs by adding material and generating the darkening of the genital plugs in certain zones. This comparative study can help to provide a wider framework of different physiological consequences related to a particular postcopulatory mechanism such as the genital plugs.

2020 ◽  
Vol 17 (35) ◽  
pp. 800-812
Author(s):  
Ilgiz DOLININ ◽  
George BAZEKIN ◽  
Evgeny SKOVORODIN ◽  
Almaz SHARIPOV ◽  
Ivan CHUDOV

Poultry farming holds a special place in ensuring the products that the consumers demand, it provides the population with essential food products,such as eggs and meat,that contain vital micro and macronutrients, proteins, lipids, and vitamins. Therefore, the issues of rational, economically feasible feeding of meat poultry, namely broiler chickens, are an urgent task. It is also essential to find effective methods of their application in order to correct the natural resistance and immune and biological reactivity of birds. The purpose of this research is to study the effect of the biological stimulant-Nucleostim on the growth and development of chickens, hematological, and immunological parameters of the blood of birds.This Biostimulant is a purified bovine spleen extract containing at least 1 mg / ml of low molecular weight peptides (nucleotides and nucleosides) formed as a result of autolysis, using dry whey and diatomite as fillers. Onthe application ofNucleostim, the gain in live weight of chickens was increased by 9.7%. At the end of the experiment, the livability of the chicks of the experimental group treated with Nucleostimcame up to 88%, compared with the 72% of the control group. The use of biostimulant had a stimulating effect on the liver of chickens confirmed by the research results presented in the article, as well as contributed to the development of the thymus in the setting of general dystrophy. Thus, it improved chicklivability and increased body weight gain. The biological stimulant-Nucleostim as an adaptogenic, anabolic, and immunostimulatory agent is promising for finding new drugs that improve the health and productivity of poultry.


Nutrients ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 1174 ◽  
Author(s):  
Takumi Kokumai ◽  
Junya Ito ◽  
Eri Kobayashi ◽  
Naoki Shimizu ◽  
Hiroyuki Hashimoto ◽  
...  

γ-Oryzanol (OZ), a bioactive phytochemical abundant in cereals such as rice, has been reported to be mainly hydrolyzed to ferulic acid (FA) in the body. Meanwhile, in our previous study, we revealed that a part of OZ is absorbed into the body and exists in its intact form. However, the comprehensive absorption profile of OZ and its metabolites (e.g., FA) after OZ intake has not been fully elucidated yet. Therefore, in this study, we measured the concentrations of OZ, FA, and FA conjugates (i.e., FA sulfate and glucuronide) in the blood of rats with the use of HPLC-MS/MS after a single oral administration of 300 µmol/kg body weight of rice bran OZ (RBOZ). As a result, intact OZ along with FA and FA conjugates existed in the blood, which implied that these constituents may all contribute to the physiological effects under OZ intake. Additionally, when an equimolar amount of FA (300 µmol/kg body weight) was administered, it was found that the absorption profile of FA was significantly different from that when RBOZ was administered.


1980 ◽  
Vol 95 (1) ◽  
pp. 21-24 ◽  
Author(s):  
G. D. Macleod ◽  
J. A. Clark

SummaryAn index of feather cover is described and applied to assess the coats of various birds. Feather area indices were calculated, by analogy with the leaf area index used by crop ecologists, as the projected area of feathers above unit area of skin. Measurements were made on samples from chickens, turkeys, pheasants, sparrows and herring gulls. The index was calculated for feather tracts on six areas of the body. An overall area index was also calculated, based on the feather cover of at least 60% of the bird's body surface. Intra- and interspecific differences were large. Between species there was a positive correlation between feather index and body weight, while within species, for poultry at least, the opposite was found. This characterization of the feather coat is discussed in terms of its possible use as an index of the insulation of feathers.


2015 ◽  
Vol 9 (2) ◽  
pp. 0-0 ◽  
Author(s):  
Фудин ◽  
N. Fudin ◽  
Хадарцев ◽  
Aleksandr Khadartsev ◽  
Несмеянов ◽  
...  

This brief report presents a description of the basic properties of mitochondria, providing a formation of energy in the human body, the ability to control a formation of energy under the influence of the Mexidol. The authors showed the main physiological effects of Mexidol in athletes and in various pathologies. A comparative study of growth performance of the pressing the barbell in the posture of lying and deadlifts in the main group of 15 people and control of 23 athletes was carried out. The athletes of the main group received the Mexidol in the dose of 200 mg/kg of body weight for 2,5 weeks. The authors found no significant increase of results in this group compared with the control and defined the importance of further studies of the properties of mitochondria and opportunities of the Mexidol in their modulation.


PEDIATRICS ◽  
1962 ◽  
Vol 30 (4) ◽  
pp. 613-621
Author(s):  
Gerard B. Odell ◽  
Sanford N. Cohen ◽  
Ellen H. Gordes

A comparative study was done to evaluate the effect of the administration of albumin on the removal of bilirubin by exchange transfusion. The amounts of bilirubin removed by simple exchange were similar when the pre-exchange concentrations in serum were alike. The administration of one gram of albumin per kilogram of body weight 1 to 2 hours prior to the procedure resulted in the removal by exchange transfusion of an average of 41% more bilirubin per kilogram of birth weight. The amount of bilirubin removed from the infants given albumin did vary from infant to infant in spite of similar pre-albumin bilirubin concentrations in serum. This was interpreted to represent variation in the total body bilirubin content, even when serum levels are alike. The amount of bilirubin removed per kilogram of birth weight was not found to be related to the etiology of the hyperbilirubinemia, the infant's age, or to the body weight at the time of the exchange. The albumin administration was associated with an increase in bilirubin concentration in serum and a decrease in capillary hematocrit value. Adipose tissue did not seem to be a major source of the additional bilirubin removed.


1980 ◽  
Vol 8 (5) ◽  
pp. 351-353 ◽  
Author(s):  
A G Wade

Diurexan® (xipamide*) 40 mg daily was substituted for Navidrex-K® (cyclopenthiazide 0.25 mg plus potassium 600 mg) or Moduretic® (amiloride hydrochloride 5 mg plus hydrochlorthiazide 50 mg) in nineteen patients with oedema of cardiac origin. Comparative efficacy and patient acceptability were examined over a 4-week treatment period. In six patients their oedema was resolved and in a further seven their oedema was markedly reduced (six patients had no overt oedema pre-trial). The body-weight of nine patients decreased by an average of 1.4 kg whilst in seven patients it remained static and in three patients it increased by an average of 1.8 kg. Thirteen of the patients preferred Diurexan at the end of the 4-week trial period, four patients had no preference and two patients preferred their previous treatment.


2021 ◽  
Vol 12 ◽  
Author(s):  
Poliane Silva Maciel ◽  
Ricardo Gonçalves ◽  
Lis Ribeiro do Valle Antonelli ◽  
Cristina Toscano Fonseca

Schistosomiasis remains one of the most important neglected tropical diseases in the world. It mainly affects developing countries, where it often coexists with malnutrition. Despite this, few studies have investigated the relationship between schistosomiasis and malnutrition. Herein, we evaluate the impact of malnutrition on experimental S. mansoni infection. Mice were divided into 5 groups: Control (Ctrl) diet (14% protein and 10% lipids), low-protein 3% (LP 3%), low-protein 8% (LP 8%), low-fat 2.5% (LF 2.5%), and low-fat 5% (LF 5%). Mice were fed with their respective diets and were infected when a difference of approximately 20% in the body weight between mice from any experimental group and mice from the control group was achieved. Nutritional, parasitological, and immunological parameters were assessed either just before infection and/or approximately 50 days later before mice were perfused. Our results showed that the 3% low-protein diet was the only one capable of establishing malnutrition in mice. Mice fed with this diet showed: (i) significant reduction in body weight and serum albumin levels before infection, (ii) decreased levels of all biochemical parameters evaluated before perfusion, (iii) decreased numbers of schistosome eggs trapped in intestines and impaired parasite fecundity, (iv) a delay in the granuloma development with a smaller granuloma area, and (v) reduced levels of IL-4 and IFN-γ in the liver. Our findings demonstrate that low protein supply leads to malnutrition in mice and impacts the cytokine milieu in the liver and granuloma formation. Additionally, the establishment of our murine malnutrition model will enable future studies aiming to better understand the complex relationships between nutrition, immune responses, and infection outcome.


Revista CERES ◽  
2017 ◽  
Vol 64 (1) ◽  
pp. 12-17 ◽  
Author(s):  
Gisele Martins de Paula Leite ◽  
Luís Eugênio Franklin Augusto ◽  
Vanessa Guedes Pereira ◽  
Adriano França Cunha ◽  
Kelly Cristine de Sousa Pontes ◽  
...  

ABSTRACT The effects of volume on lumbosacral epidural block in dogs were evaluated using two different doses of 2% lidocaine. Ten adult dogs, without defined breed, were subjected to two different anesthetic protocols. In the first, the local anesthetic was calculated based on the body weight (GP), wherein 1.0 mL of local anesthetic was used for each 3.5 kg; in the second protocol, the dose was stipulated according to the occipito-coccygeal (DG) distance with 1.5mL of local anesthetic for every 10 cm of distance. The available time, recovery period, extent of block, and rectal temperature were measured. After the analysis of the results, it was possible to verify that there was an increase in the time in GD when compared with GP, due to the greater volume administered in that group. The recovery period remained similar in both groups, despite the use of different doses. Regarding the extent of blockade, there was an increase in GD in relation to GP due to the increase of the local anesthetic dose. On the other hand, the rectal temperature presented a difference between the groups, remaining lower and below the reference values for the species in GD compared with GP at all moments analyzed, possibly due to a sympathetic action triggered by the cranial extension of the epidural block. It could be concluded that when a longer time and a more cranial blockade of epidural anesthesia are desired, the anesthesia volume should be based on the occipito-coccygeal distance, however, observing the rectal temperature.


2011 ◽  
Vol 14 (2) ◽  
pp. 219-225
Author(s):  
T. Bakuła ◽  
Ł. Lis ◽  
Z. Iwaniuk ◽  
Z. Ordyński

The effect of diets supplemented with fish broth and fish oil on the health of weaners The aim of this study was to determine the effect of fish-based feed materials, as a source of readily available protein contained in fish broth and essential polyunsaturated fatty acids (PUFAs) found in fish oil, on the health of piglets and rearing results. The experiment was conducted on a commercial pig fattening farm. The study involved a total of 80 weaners with an approximate body weight of 15 kg. The experiment was carried out over a period of 40 days. Feed samples were subjected to laboratory analyses. Blood samples were collected from experimental group animals to determine serum biochemical and immunological parameters. The body weight gains of weaners, mortality rates and average feed intake per animal were calculated for the entire experimental period. The addition of fish broth and fish oil significantly improved the n3:n6 fatty acid ratio in diets. The presence of EPA and DHA in the experimental diet could have had a positive health effect on piglets, comparable with that exerted by therapeutic doses of zinc often administered to pigs of this age group. During the experiment, feed conversion ratio (FCR) gain was considerably reduced in the experimental group, with similar daily gains in the control and experimental group.


2014 ◽  
Vol 84 (1-2) ◽  
pp. 5-11 ◽  
Author(s):  
Eun Y. Jung ◽  
Sung C. Jun ◽  
Un J. Chang ◽  
Hyung J. Suh

Previously, we have found that the addition of L-ascorbic acid to chitosan enhanced the reduction in body weight gain in guinea pigs fed a high-fat diet. We hypothesized that the addition of L-ascorbic acid to chitosan would accelerate the reduction of body weight in humans, similar to the animal model. Overweight subjects administered chitosan with or without L-ascorbic acid for 8 weeks, were assigned to three groups: Control group (N = 26, placebo, vehicle only), Chito group (N = 27, 3 g/day chitosan), and Chito-vita group (N = 27, 3 g/day chitosan plus 2 g/day L-ascorbic acid). The body weights and body mass index (BMI) of the Chito and Chito-vita groups decreased significantly (p < 0.05) compared to the Control group. The BMI of the Chito-vita group decreased significantly compared to the Chito group (Chito: -1.0 kg/m2 vs. Chito-vita: -1.6 kg/m2, p < 0.05). The results showed that the chitosan enhanced reduction of body weight and BMI was accentuated by the addition of L-ascorbic acid. The fat mass, percentage body fat, body circumference, and skinfold thickness in the Chito and Chito-vita groups decreased more than the Control group; however, these parameters were not significantly different between the three groups. Chitosan combined with L-ascorbic acid may be useful for controlling body weight.


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