Propolis extract and oregano essential oil as biofungicides for garlic seed‐cloves: in vitro assays and synergistic interaction against Penicillium allii

2021 ◽  
Author(s):  
Irene L. Cibanal ◽  
Leticia A. Fernández ◽  
Ana P. Murray ◽  
Cecilia N. Pellegrini ◽  
Liliana M. Gallez
Keyword(s):  
Essential Oil ◽  
Synergistic Interaction ◽  
In Vitro Assays ◽  
Propolis Extract ◽  
Oregano Essential Oil

scholarly journals Individual and Combined Coatings of Chitosan and Carnauba Wax with Oregano Essential Oil to Avoid Water Loss and Microbial Decay of Fresh Cucumber

Coatings ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 614
Author(s):  
María Melissa Gutiérrez-Pacheco ◽  
Luis Alberto Ortega-Ramírez ◽  
Brenda Adriana Silva-Espinoza ◽  
Manuel Reynaldo Cruz-Valenzuela ◽  
Gustavo Adolfo González-Aguilar ◽  
...  
Keyword(s):  
Weight Loss ◽  
Essential Oil ◽  
Transmission Rate ◽  
Infrared Analysis ◽  
Absorption Bands ◽  
Oregano Essential Oil ◽  
Carnauba Wax ◽  
Bacteria And Fungi ◽  
Combined Coatings

The objective of the present study is to evaluate the effect of individual and combined coatings of chitosan (0.008 g·mL−1) and carnauba wax (0.1 g·mL−1) with oregano essential oil (OEO, 0.08 g·mL−1) to reduce dehydration and microbial decay of fresh cucumbers stored at 10 °C. Chitosan-OEO-wax films showed the lowest water vapor transmission rate (0.141 g·m−2·h−1), compared to single chitosan films (0.257 g·m−2·h−1). While chitosan-OEO films completely inhibited the in vitro growth of Alternaria alternata and reduced the growth of Salmonella Typhimurium, Escherichia coli O157:H7, mesophilic bacteria, and fungi isolated from decayed cucumbers. Besides, the infrared analysis of chitosan-OEO-wax films showed shifts in O–H and N–H absorption bands, indicating possible hydrogen bonding between the components. Wax and wax-OEO were the most effective coatings to prevent weight loss in cucumbers during 15 days of storage at 10 °C, while the most effective antimicrobial treatments were chitosan and chitosan-OEO. Therefore, these results showed that carnauba wax and carnauba wax-OEO coatings were the most effective in weight loss, whereas chitosan and chitosan-OEO were the most effective to reduce the microbial load of the treated fresh cucumber.

scholarly journals Premna odorata: Seasonal Metabolic Variation in the Essential Oil Composition of Its Leaf and Verification of Its Anti-Ageing Potential via In Vitro Assays and Molecular Modelling

Biomolecules ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 879
Author(s):  
Ahmed E. Altyar ◽  
Mohamed L. Ashour ◽  
Fadia S. Youssef
Keyword(s):  
Gas Chromatography ◽  
Essential Oil ◽  
Molecular Modelling ◽  
Essential Oil Composition ◽  
In Vitro Assays ◽  
Oil Composition ◽  
Different Seasons ◽  
Metabolic Variation ◽  
Active Centres

The metabolic variation in the essential oil composition of Premna odorata leaves obtained from different seasons was quantitatively and qualitatively determined employing GC/MS (Gas Chromatography coupled with Mass Spectrometry) and GC/FID (Gas chromatography equipped with flame ionization detector) techniques. It displayed the existence of 97 constituents accounting for 94.19%, 92.27%, 91.95% and 92.63% for POS (spring), POM (summer), POA (autumn) and POW (winter) whole essential oils. β-Caryophyllene constituting the main metabolite in the oil in the different seasons. To better visualize the differences between them, GC data were exposed to chemometric analysis. A PCA (principal component analysis) score plot revealed the closeness of POS and POW. Molecular modelling on collagenase, elastase and hyaluronidase enzymes active centres shows that different compounds existing in the essential oil of Premna odorata leaves shows binding to the active sites with variable degrees that suggested its anti-ageing potential. Palmitic acid displayed the highest fitting for both the collagenase and elastase active centres in both pH-based and rule-based ionization methods with ∆G equals −78.27 and −44.77 kcal/mol, respectively; meanwhile, heptacosane showed the highest fitting score in the hyaluronidase centre with ∆G = −43.78 kcal/mol. In vitro assays consolidates the obtained modelling studies in which essential oil shows considerable anti-elastase and anti-hyaluronidase potential as evidenced by their IC50 values being 49.3 and 37.7 μg/mL, respectively; meanwhile, the essential oil of Premna odorata leaves displayed mild anti-collagenase potential. Thus, it can be concluded that Premna odorata could serve as a promising anti-ageing naturally occurring drug that could be effectively incorporated by pharmaceutical industries in cosmetics combating ageing and skin wrinkling.

scholarly journals Acantholippia salsoloides: Phytochemical Composition and Biological Potential of a Thujonic Population

2019 ◽  
Vol 14 (6) ◽  
pp. 1934578X1985854
Author(s):  
Liliana Celaya ◽  
Carmen Viturro ◽  
Luís R. Silva
Keyword(s):  
Essential Oil ◽  
High Performance ◽  
Micrococcus Luteus ◽  
Pharmaceutical Formulations ◽  
In Vitro Assays ◽  
Array Detector ◽  
Biological Potential ◽  
Polar Extracts ◽  
Hydroethanolic Extract

Acantholippia salsoloides (Verbenaceae) is an aromatic plant widespread in the Andean region. The infusion (leaves and flowers) is widely used as a digestive stimulant as well as for the treatment of various diseases in traditional medicine. A. salsoloides attributes its common name “rica-rica” to the fresh and sweet fragrance of the plant. In this work, 2 different polar extracts and the essential oil of a selected rica-rica population were studied. The phenolic composition was analyzed by high-performance liquid chromatography diode array detector; the essential oil profile was determined by gas-chromatography ion-trap mass spectrometry/flame ionization detection. For all extracts, the antibacterial potential was performed by in vitro assays; the antioxidant and α-glucosidase inhibition were determined in decoction and hydroethanolic extracts. The volatile profile allowed the identification of 26 volatile compounds, β-thujone (84%) being the major one in this rica-rica population. Eighteen phenolic compounds were identified; isoferulic acid (16%-18%) and cynaroside (45%-47%) were the larger ones. In a general way, the hydroethanolic extract was more active against Staphylococcus aureus and Micrococcus luteus (minimum inhibitory concentrations= 0.3- 1.3 mg/mL). Both polar extracts have strong antiradical activities although decoction extract proved to be more active against DPPH· (half-maximal inhibitory concentration [IC50] =36 µg/mL) and O2•− (IC50 =28 µg/mL) while hydroethanolic extract shows higher action over α-glucosidase (IC50 =217 µg/mL). The results suggest that A. salsoloides leaves and flowers may be an interesting source of natural antioxidants, antidiabetics, or antimicrobials, and could be used in dietary supplements, medicinal products and pharmaceutical formulations.

Reduced Susceptibility of Salmonella Typhimurium Strains to Oregano Essential Oil and Enrofloxacin: An In Vitro Assay

2020 ◽  
Vol 17 (1) ◽  
pp. 29-34
Author(s):  
Ana Lucía Solarte ◽  
Rafael Jesús Astorga ◽  
Fabiana C. de Aguiar ◽  
Carmen Tarradas ◽  
Inmaculada Luque ◽  
...  
Keyword(s):  
Essential Oil ◽  
Salmonella Typhimurium ◽  
In Vitro Assay ◽  
Vitro Assay ◽  
Oregano Essential Oil

Two-step method for encapsulation of oregano essential oil in chitosan nanoparticles: Preparation, characterization and in vitro release study

2013 ◽  
Vol 95 (1) ◽  
pp. 50-56 ◽  
Author(s):  
Seyed Fakhreddin Hosseini ◽  
Mojgan Zandi ◽  
Masoud Rezaei ◽  
Farhid Farahmandghavi
Keyword(s):  
Essential Oil ◽  
Chitosan Nanoparticles ◽  
In Vitro Release ◽  
Step Method ◽  
Oregano Essential Oil ◽  
Release Study ◽  
Vitro Release

Activity of chitosan-montmorillonite bionanocomposites incorporated with rosemary essential oil: From in vitro assays to application in fresh poultry meat

2019 ◽  
Vol 89 ◽  
pp. 241-252 ◽  
Author(s):  
Victor Gomes Lauriano Souza ◽  
João R.A. Pires ◽  
Érica Torrico Vieira ◽  
Isabel M. Coelhoso ◽  
Maria Paula Duarte ◽  
...  
Keyword(s):  
Essential Oil ◽  
Poultry Meat ◽  
In Vitro Assays ◽  
Rosemary Essential Oil

scholarly journals Effect of the matrix system in the delivery and in vitro bioactivity of microencapsulated Oregano essential oil

2012 ◽  
Vol 110 (2) ◽  
pp. 190-199 ◽  
Author(s):  
Sara Beirão da Costa ◽  
Claudia Duarte ◽  
Ana I. Bourbon ◽  
Ana C. Pinheiro ◽  
Ana Teresa Serra ◽  
...  
Keyword(s):  
Essential Oil ◽  
Matrix System ◽  
In Vitro Bioactivity ◽  
Oregano Essential Oil ◽  
The Matrix

scholarly journals Polysaccharide-Based Multilayer Nano-Emulsions Loaded with Oregano Oil: Production, Characterization, and In Vitro Digestion Assessment

Nanomaterials ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 878
Author(s):  
Luz Espinosa-Sandoval ◽  
Claudia Ochoa-Martínez ◽  
Alfredo Ayala-Aponte ◽  
Lorenzo Pastrana ◽  
Catarina Gonçalves ◽  
...  
Keyword(s):  
Particle Size ◽  
Essential Oil ◽  
Zeta Potential ◽  
In Vitro Digestion ◽  
High Energy ◽  
Oregano Essential Oil ◽  
Emulsification Capacity ◽  
Octenyl Succinic Anhydride ◽  
Nano Emulsion

The food industry has increased its interest in using “consumer-friendly” and natural ingredients to produce food products. In the case of emulsifiers, one of the possibilities is to use biopolymers with emulsification capacity, such as octenyl succinic anhydride modified starch, which can be used in combination with other polysaccharides, such as chitosan and carboxymethylcellulose, in order to improve the capacity to protect bioactive compounds. In this work, multilayer nano-emulsion systems loaded with oregano essential oil were produced by high energy methods and characterized. The process optimization was carried out based on the evaluation of particle size, polydispersity index, and zeta potential. Optimal conditions were achieved for one-layer nano-emulsions resulting in particle size and zeta potential of 180 nm and −42 mV, two layers (after chitosan addition) at 226 nm and 35 mV, and three layers (after carboxymethylcellulose addition) of 265 nm and −1 mV, respectively. The encapsulation efficiency of oregano essential oil within nano-emulsions was 97.1%. Stability was evaluated up to 21 days at 4 and 20 °C. The three layers nano-emulsion demonstrated to be an efficient delivery system of oregano essential oil, making 40% of the initial oregano essential oil available versus 13% obtained for oregano essential oil in oil, after exposure to simulated digestive conditions.

scholarly journals Herbicide Potential of Selected Essential Oils From Plants of Lamiaceae and Asteraceae Families

2021 ◽  
Vol 74 ◽  
Author(s):  
Milena Nikolova ◽  
Boryanka Traykova ◽  
Elina Yankova-Tsvetkova ◽  
Tatyana Stefanova ◽  
Anatoli Dzhurmanski ◽  
...  
Keyword(s):  
Seed Germination ◽  
Essential Oil ◽  
Essential Oils ◽  
Trifolium Pratense ◽  
Herbicidal Activity ◽  
Field Conditions ◽  
In Vitro Assays ◽  
Gas Chromatography Mass Spectrometry ◽  
Monarda Fistulosa

Abstract Essential oils from Monarda fistulosa L., Satureja pilosa Vel., Origanum vulgare subsp. hirtum Ietswaart. (Greek oregano), Micromeria dalmatica Benth., Thymus longedentatus (Degen & Urum.) Ronniger, and Artemisa campestris L. were evaluated as inhibitors of seed germination in target plants Lolium perenne L. and Trifolium pratense L. using in vitro assays. The essential oils were applied on the seeds as aqueous solutions at concentrations ranging from 0.5–3.0 µL/mL. Complete inhibition was established at a concentration of 1.5 µL/mL with the most effective essential oils. Oregano oil was evaluated for its inhibitory activity on seed germination under field conditions; the essential oil was applied as an aqueous solution at concentrations of 3, 5, and 10 µL/mL on superabsorbent Terawet. The mass obtained was mixed with the seeds of the target plants and planted in a field. After 1 month, the results were evaluated based on the weight of the aerial parts of the plants from control and experimental areas. At the highest concentration studied, the weight of the plants was 77% lower in the experimental areas than in the controls. The chemical composition of the essential oils was analyzed using gas chromatography–mass spectrometry. The results showed that carvacrol-rich essential oils had a strong inhibitory effect on seed germination. The inclusion of the essential oil on a superabsorbent was a good way to preserve its herbicidal activity under field conditions as this prevented its rapid evaporation.

scholarly journals Evaluation of antimicrobial activities of plant aqueous extracts against Salmonella Typhimurium and their application to improve safety of pork meat

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Alkmini Gavriil ◽  
Evangelia Zilelidou ◽  
Angelis-Evangelos Papadopoulos ◽  
Danae Siderakou ◽  
Konstantinos M. Kasiotis ◽  
...  
Keyword(s):  
Essential Oil ◽  
Edible Coatings ◽  
Aqueous Extracts ◽  
Pork Meat ◽  
Promising Alternative ◽  
Oregano Essential Oil ◽  
Corn Silk ◽  
Strain Dependent ◽  
By Products

AbstractNine odorless laboratory-collected hydro-distilled aqueous extracts (basil, calendula, centrifuged oregano, corn silk, laurel, oregano, rosemary, spearmint, thyme) and one industrial steam-distilled oregano hydrolate acquired as by-products of essential oils purification were screened for their in vitro antimicrobial activity against three Salmonella Typhimurium strains (4/74, FS8, FS115) at 4 and 37 °C. Susceptibility to the extracts was mainly plant- and temperature-dependent, though strain dependent effects were also observed. Industrial oregano hydrolate eliminated strains immediately after inoculation, exhibiting the highest antimicrobial potential. Hydro-distilled extracts eliminated/reduced Salmonella levels during incubation at 4 °C. At 37 °C, oregano, centrifuged oregano, thyme, calendula and basil were bactericidal while spearmint, rosemary and corn silk bacteriostatic. A strain-dependent effect was observed for laurel. The individual or combined effect of marinades and edible coatings prepared of industrial hydrolate and hydro-distilled oregano extracts with or without oregano essential oil (OEO) was tested in pork meat at 4 °C inoculated with FS8 strain. Lower in situ activity was observed compared to in vitro assays. Marinades and edible coatings prepared of industrial oregano hydrolate + OEO were the most efficient in inhibiting pathogen. Marination in oregano extract and subsequent coating with either 50% oregano extract + OEO or water + OEO enhanced the performance of oregano extract. In conclusion, by-products of oregano essential oil purification may be promising alternative antimicrobials to pork meat stored under refrigeration when applied in the context of multiple hurdle approach.

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