A Steady-State Filtration Model for Transluminal Water Movement in Small and Large Blood Vessels

1985 ◽  
Vol 107 (2) ◽  
pp. 123-130 ◽  
Author(s):  
G. Tzeghai ◽  
S. Weinbaum ◽  
R. Pfeffer
Keyword(s):  
Endothelial Cells ◽  
Steady State ◽  
Pressure Distribution ◽  
Hydraulic Resistance ◽  
Wall Thickness ◽  
Matrix Theory ◽  
Water Movement ◽  
Interaction Layer ◽  
Highly Nonlinear ◽  
The Media

It is now generally accepted that the intercellular cleft between adjacent endothelial cells is the primary pathway for the transluminal movement of water and small ions in the vasculature. A steady-state theoretical model has been developed to show quantitatively how the geometry of the intercellular cleft between adjacent endothelial cells is related to both the water movement and pressure distribution in the subendothelial space and to examine how the existence of a subendothelial interaction layer affects the hydraulic resistance of the media of vessels of varying wall thickness. The velocity and pressure fields in the media are described using porous matrix theory based on Darcy’s law and a lubrication-type analysis is used to describe the flow in a variable geometry intercellular cleft. These two equations are solved simultaneously to determine the unknown pressure distribution beneath the endothelium and the flow in the arterial media. Application of this model shows that, when the tight junction in the cleft is 26 Å or less, more than half of the total hydraulic resistance of the wall occurs across the endothelial cell monolayer, for a vessel whose wall thickness is less than 0.02 cm. This finding is in good agreement with the experimental findings of Vargas, et al. (1978) for rabbit aorta. Contrary to previous belief, the model shows that the filtration resistance of an arterial wall with intact endothelium does not scale linearly with wall thickness due to the highly nonlinear resistance of the endothelial interaction layer.

Fibrinolysis in Decidual Spiral Arteries in Late Pregnancy

1978 ◽  
Vol 39 (03) ◽  
pp. 751-758 ◽  
Author(s):  
B L Sheppard ◽  
J Bonnar
Keyword(s):  
Electron Microscopy ◽  
Endothelial Cells ◽  
Fibrinolytic Activity ◽  
Physiological Mechanism ◽  
Late Pregnancy ◽  
Full Term ◽  
Fibrin Deposition ◽  
Placental Villi ◽  
The Media ◽  
Slide Technique

SummaryThe fibrinolytic activity of the intimal cells of decidual spiral arteries and the syncytium of placental villi was studied by electron microscopy in ten normal full-term human pregnancies using a modification of the fibrin slide technique. Endothelial cells lining the intima of the decidual spiral arteries showed a considerably greater fibrinolytic activity than intimal cytotrophoblast and the syncytiotrophoblast showed no activity.The replacement of endothelial cells by an intimal lining of cytotrophoblast, and the presence of cytotrophoblast in the media, appears to play an important role in the reduction of the fibrinolytic activity of the vessel. This inhibition of fibrinolytic activity in the utero-placental arteries may be the physiological mechanism which controls fibrin deposition in these vessels and on the placental villi.

Localization of Fibrinolytic Activators and Inhibitors in Normal and Atherosclerotic Vessels

1996 ◽  
Vol 75 (06) ◽  
pp. 933-938 ◽  
Author(s):  
Marten Fålkenberg ◽  
Johan Tjärnstrom ◽  
Per Örtenwall ◽  
Michael Olausson ◽  
Bo Risberg
Keyword(s):  
Endothelial Cells ◽  
Plasminogen Activator ◽  
Plasminogen Activator Inhibitor ◽  
Vasa Vasorum ◽  
Type Plasminogen Activator ◽  
Urokinase Type Plasminogen Activator ◽  
Tissue Plasminogen ◽  
The Media ◽  
Activator Inhibitor ◽  
Pai 1

SummaryLocal fibrinolytic changes in atherosclerotic arteries have been suggested to influence plaque growth and promote mural thrombosis on ruptured or ulcerated plaques. Increased levels of plasminogen activator inhibitor (PAI-1) have been found in atherosclerotic arteries. In this study tissue plasminogen activator (t-PA), urokinase-type plasminogen activator (u-PA) and PAI-1 were localized in arterial biopsies of healthy and atherosclerotic vessels by immunohistochemis-try. The expression of fibrinolytic regulators was related to the distribution of endothelial cells (EC) and macrophages. Results: t-PA was expressed in vasa vasorum. PAI-1 was positive in endothelial cells, in the media and in the adventitia. Increased expression of t-PA, u-PA and PAI-1 was found in atherosclerotic vessels. t-PA, u-PA, PAI-1 and macrophages were co-localized in plaques. These results support the concept that macrophages can be important in the local regulation of fibrinolysis in atherosclerotic vessels.

scholarly journals Parameters Estimation and Prediction of Water Movement and Solute Transport in Layered, Variably Saturated Soils Using the Ensemble Kalman Filter

Water ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 1520
Author(s):  
Zheng Jiang ◽  
Quanzhong Huang ◽  
Gendong Li ◽  
Guangyong Li
Keyword(s):  
Kalman Filter ◽  
Solute Transport ◽  
Ensemble Kalman Filter ◽  
Water Movement ◽  
Parameters Estimation ◽  
Saturated Soils ◽  
Hydrological Models ◽  
State Variables ◽  
Ensemble Size ◽  
Highly Nonlinear

The parameters of water movement and solute transport models are essential for the accurate simulation of soil moisture and salinity, particularly for layered soils in field conditions. Parameter estimation can be achieved using the inverse modeling method. However, this type of method cannot fully consider the uncertainties of measurements, boundary conditions, and parameters, resulting in inaccurate estimations of parameters and predictions of state variables. The ensemble Kalman filter (EnKF) is well-suited to data assimilation and parameter prediction in Situations with large numbers of variables and uncertainties. Thus, in this study, the EnKF was used to estimate the parameters of water movement and solute transport in layered, variably saturated soils. Our results indicate that when used in conjunction with the HYDRUS-1D software (University of California Riverside, California, CA, USA) the EnKF effectively estimates parameters and predicts state variables for layered, variably saturated soils. The assimilation of factors such as the initial perturbation and ensemble size significantly affected in the simulated results. A proposed ensemble size range of 50–100 was used when applying the EnKF to the highly nonlinear hydrological models of the present study. Although the simulation results for moisture did not exhibit substantial improvement with the assimilation, the simulation of the salinity was significantly improved through the assimilation of the salinity and relative solutetransport parameters. Reducing the uncertainties in measured data can improve the goodness-of-fit in the application of the EnKF method. Sparse field condition observation data also benefited from the accurate measurement of state variables in the case of EnKF assimilation. However, the application of the EnKF algorithm for layered, variably saturated soils with hydrological models requires further study, because it is a challenging and highly nonlinear problem.

Ascorbate and glutathione homeostasis in vascular smooth muscle cells: cooperation with endothelial cells

1998 ◽  
Vol 275 (4) ◽  
pp. C1031-C1039 ◽  
Author(s):  
Ilia Voskoboinik ◽  
Karin Söderholm ◽  
Ian A. Cotgreave
Keyword(s):  
Endothelial Cells ◽  
Smooth Muscle ◽  
Steady State ◽  
Smooth Muscle Cells ◽  
Umbilical Vein ◽  
State Level ◽  
Muscle Cells ◽  
Free Access ◽  
Steady State Level ◽  
Human Umbilical Vein

Human umbilical vein smooth muscle cells (HUVSMCs) utilize extracellular cystine, glutathione (GSH), and N-acetylcysteine (NAC) to synthesize cellular GSH. Extracellular cystine was effective from 5 μM, whereas GSH and NAC were required at 100 μM for comparable effects. The efficacy of extracellular GSH was dependent on de novo GSH synthesis, indicating a dependence on cellular γ-glutamyltransferase (glutamyl transpeptidase). Coculture of syngenetic HUVSMCs and corresponding human umbilical vein endothelial cells (HUVECs) on porous supports restricted cystine- or GSH-stimulated synthesis of HUVSMC GSH when supplied on the “luminal” endothelial side. Thus HUVSMC GSH rapidly attained a steady-state level below that achieved in the absence of interposed HUVECs. HUVSMCs also readily utilize both reduced ascorbate (AA) and oxidized dehydroascorbate (DHAA) over the range 50–500 μM. Phloretin effectively blocked both AA- and DHAA-stimulated assimilation of intracellular AA, indicating a role for a glucose transporter in their transport. Uptake of extracellular AA was also sensitive to extracellular, but not intracellular, thiol depletion. When AA was applied to the endothelial side of the coculture model, assimilation of intracellular AA in HUVSMCs was restricted to a steady-state level below that achieved by free access.

scholarly journals Transmigration across a Steady-State Blood–Brain Barrie Induces Activation of Circulating Dendritic Cells Partly Mediated by Actin Cytoskeletal Reorganization

Membranes ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 700
Author(s):  
Megha Meena ◽  
Mats Van Delen ◽  
Maxime De Laere ◽  
Ann Sterkens ◽  
Coloma Costas Romero ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Dendritic Cells ◽  
Steady State ◽  
T Cell ◽  
Cytoskeletal Reorganization ◽  
Blood Brain ◽  
Stimulatory Capacity ◽  
The Central Nervous System ◽  
Cell Stimulatory Capacity

The central nervous system (CNS) is considered to be an immunologically unique site, in large part given its extensive protection by the blood–brain barrier (BBB). As our knowledge of the complex interaction between the peripheral immune system and the CNS expands, the mechanisms of immune privilege are being refined. Here, we studied the interaction of dendritic cells (DCs) with the BBB in steady–state conditions and observed that transmigrated DCs display an activated phenotype and stronger T cell-stimulatory capacity as compared to non-migrating DCs. Next, we aimed to gain further insights in the processes underlying activation of DCs following transmigration across the BBB. We investigated the interaction of DCs with endothelial cells as well as the involvement of actin cytoskeletal reorganization. Whereas we were not able to demonstrate that DCs engulf membrane fragments from fluorescently labelled endothelial cells during transmigration across the BBB, we found that blocking actin restructuring of DCs by latrunculin-A significantly impaired in vitro migration of DC across the BBB and subsequent T cell-stimulatory capacity, albeit no effect on migration-induced phenotypic activation could be demonstrated. These observations contribute to the current understanding of the interaction between DCs and the BBB, ultimately leading to the design of targeted therapies capable to inhibit autoimmune inflammation of the CNS.

Morphological Study of Carotid Artery of WHHL Rabbit after Balloon Dilatation

1998 ◽  
Vol 4 (1_suppl) ◽  
pp. 183-186
Author(s):  
T. Yokouchi ◽  
S. Iwabuchi ◽  
A. Tomiyama ◽  
H. Samejima ◽  
K. Takahashi ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Carotid Artery ◽  
Morphological Study ◽  
Balloon Dilatation ◽  
Carotid Bifurcation ◽  
Morphological Alteration ◽  
Elastic Fibers ◽  
Intimal Thickening ◽  
The Media ◽  
Over Time

We performed balloon dilatation in the carotid artery of WHHL rabbits and examined subsequent morphological alteration over time. The balloon was inserted as far as the carotid bifurcation and observations were made on the morphological alteration after dilatation in the atherosclerotic intimal thickening from immediately after balloon dilatation over a period of ten months. Immediately after balloon dilatation, endothelial cells came of circularly and stretching, fragmentation off elastic fibers and coming off of smooth muscle cells of the media were confirmed. No change in the degree of thickening after dilatation was seen in the atherosclerotic intimal thickening of the carotid bifurcation. Three weeks later, endothelium covering except some parts, circular neointima and fibrosis of the media were observed; foamy cells had accumulated in the upper layer of the atherosclerotic intimal thickening, and that region was not yet covered with endothelial cells. The progress of fibrous intimal thickening so as to keep the lumen smooth was seen up to six months later, but foamy cells were not found in the neointima.

scholarly journals Characterizing water fingering phenomena in soils using magnetic resonance imaging and multifractal theory

2009 ◽  
Vol 16 (1) ◽  
pp. 159-168 ◽  
Author(s):  
A. Posadas ◽  
R. Quiroz ◽  
A. Tannús ◽  
S. Crestana ◽  
C. M. Vaz
Keyword(s):  
Magnetic Resonance Imaging ◽  
Steady State ◽  
Magnetic Resonance ◽  
Preferential Flow ◽  
Water Movement ◽  
Three Dimensions ◽  
Water Infiltration ◽  
Resonance Imaging ◽  
Flow Process ◽  
Multifractal Theory

Abstract. The study of water movement in soils is of fundamental importance in hydrologic science. It is generally accepted that in most soils, water and solutes flow through unsaturated zones via preferential paths or fingers. This paper combines magnetic resonance imaging (MRI) with both fractal and multifractal theory to characterize preferential flow in three dimensions. A cubic double-layer column filled with fine and coarse textured sand was placed into a 500 gauss MRI system. Water infiltration through the column (0.15×0.15×0.15 m3) was recorded in steady state conditions. Twelve sections with a voxel volume of 0.1×0.1×10 mm3 each were obtained and characterized using fractal and multifractal theory. The MRI system provided a detailed description of the preferential flow under steady state conditions and was also useful in understanding the dynamics of the formation of the fingers. The f(α) multifractal spectrum was very sensitive to the variation encountered at each horizontally-oriented slice of the column and provided a suitable characterization of the dynamics of the process identifying four spatial domains. In conclusion, MRI and fractal and multifractal analysis were able to characterize and describe the preferential flow process in soils. Used together, the two methods provide a good alternative to study flow transport phenomena in soils and in porous media.

scholarly journals Application of Analytical Solution to Steady-State Temperature Field by Double-Row-Pipe Freezing and Verification with Field Measurement: A Case Study of Connected Aisle

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Zequn Hong ◽  
Xiangdong Hu
Keyword(s):  
Temperature Field ◽  
Steady State ◽  
Analytical Solution ◽  
Wall Thickness ◽  
Field Measurement ◽  
Field Analysis ◽  
Shield Tunnel ◽  
Double Row ◽  
Steady State Temperature ◽  
Frozen Wall

In order to solve the problem of sealing water and bearing capacity of a connected aisle in an underwater shield tunnel, a double-circle horizontal freezing method was adopted for ground reinforcement in the connected aisle of Maliuzhou Tunnel, which is China’s first shield tunnel with superlarge diameter built in a composite stratum. This paper proposed a new double-row-pipe freezing model for the calculation of frozen wall thickness based on analytical solution to steady-state temperature field. Besides, field measurement and transient numerical studies of the active freezing period were also carried out to study the freeze-sealing effect. The results show that frozen wall thickness obtained by analytical solutions agrees well with numerical simulation results, which verifies the applicability of the newly proposed calculation method. Field analysis indicates that soil temperature gradually approaches a stable value which is far below the freezing point, and a reliable water-sealing curtain can be formed around the designed connected aisle. Maximum impact of soil excavation on the frozen wall is about 10°C, and reducing exposure time of excavation surface can effectively alleviate the weakening of frozen wall. To obtain comprehensive analysis for freezing wall thickness, a more reasonable arrangement of temperature-measuring holes is expected in future freezing engineering.

Weak Instability of Chen–Ricles Explicit Method for Structural Dynamics

2018 ◽  
Vol 13 (5) ◽  
Author(s):  
Shuenn-Yih Chang
Keyword(s):  
Steady State ◽  
Nonlinear Systems ◽  
High Frequency ◽  
Explicit Method ◽  
Order Accuracy ◽  
Practical Applications ◽  
Correction Scheme ◽  
Highly Nonlinear ◽  
Steady State Responses ◽  
State Responses

Although the Chen–Ricles (CR) explicit method (CRM) (proposed by Chen and Ricles) has been claimed to have desired numerical properties, such as unconditional stability, explicit formulation, and second-order accuracy, it also shows some unusual properties, such as a less accuracy of solving highly nonlinear systems, a high-frequency overshoot in steady-state responses, and a weak instability. A correction scheme by adjusting the displacement difference equation with a loading term can be employed to extinguish the high-frequency overshoot in steady-state responses. However, there is still no way to get rid of the weak instability and to improve the less accuracy of solving highly nonlinear systems. It is recognized that a weak instability might result in inaccurate solutions or numerical explosions. Hence, the practical applications of CRM are strictly limited.

scholarly journals Expression of tissue factor pathway inhibitor by cultured endothelial cells in response to inflammatory mediators

Blood ◽  
1992 ◽  
Vol 79 (12) ◽  
pp. 3219-3226 ◽  
Author(s):  
A Ameri ◽  
MN Kuppuswamy ◽  
S Basu ◽  
SP Bajaj
Keyword(s):  
Endothelial Cells ◽  
Blood Serum ◽  
Tissue Factor ◽  
Inflammatory Mediators ◽  
Whole Blood ◽  
Tissue Factor Pathway Inhibitor ◽  
Binding Motif ◽  
Cultured Endothelial Cells ◽  
The Media ◽  
Tissue Factor Pathway

Abstract We recently proposed that endothelium may represent the primary physiologic site of synthesis of the tissue factor pathway inhibitor (TFPI). In support of this conclusion, we have now found that the poly(A)+ RNAs obtained from rabbit and bovine lung tissues contain abundant amounts of TFPI messenger RNAs (mRNAs), whereas the poly(A)+ RNAs obtained from the liver of these animals contain less than 5% of that found in the lung tissues. Because inflammatory mediators are known to upregulate tissue factor (TF) expression by the endothelium, we have examined the effect of these agents on the TFPI expression by the cultured endothelial cells. When cultured human umbilical vein endothelial cells were stimulated (in 10% fetal bovine serum) with phorbol myristate acetate (PMA), endotoxin, interleukin-1, or tumor necrosis factor-alpha, the TF mRNA increased approximately 7- to 10- fold within 2 to 4 hours. Unstimulated cells constitutively expressed TFPI mRNA and its levels either did not change or increased slightly (up to 1.5-fold) upon stimulation with these inflammatory agents. TF mRNA abruptly declined to a negligible level and the TFPI mRNA returned essentially to the basal level at approximately 24 hours. The membrane- bound TF clotting activity of induced cells peaked between 4 and 8 hours, and finally declined. The cumulative TFPI activity secreted into the media was either unchanged or slightly higher in the induced cell cultures as compared with that present in the noninduced cultures. Endothelial cells were also cultured in 10% heat-inactivated human serum derived from plasma or whole blood. TFPI secreted into the media containing whole blood serum was consistently higher (approximately 1.5- fold at 8 hours) than that secreted into the media supplemented with serum obtained from plasma lacking the formed elements; these cells also expressed similarly increased levels of TFPI mRNA. Moreover, PMA- stimulated cells cultured in whole blood serum expressed modestly increased levels of TFPI mRNA (approximately 1.5-fold); supernatants from these cells also contained similarly increased TFPI activity. Cumulatively, our data indicate that, unlike thrombomodulin and fibrinolytic enzymes synthesized by the endothelial cells, TFPI synthesis is not downregulated and may be slightly upregulated during an inflammatory response. Inspection of the 5′ flanking region of the TFPI gene showed a conserved GATA-binding motif located approximately 400 bp upstream of the proposed transcription initiation site(s). This motif by binding to the GATA-2 transcriptional factor may keep the endothelium in an ‘on’ state for constitutive expression of TFPI.(ABSTRACT TRUNCATED AT 400 WORDS)

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